BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00002567
         (3165 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum] >gi|11...  1427   0.0  
gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]      1376   0.0  
ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythra...  1209   0.0  
gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythra...  1209   0.0  
gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]   1194   0.0  
emb|CDP13767.1| unnamed protein product [Coffea canephora]           1162   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1141   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1129   0.0  
ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea...  1127   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1122   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1119   0.0  
dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]            1115   0.0  
dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. g...  1112   0.0  
dbj|BAG31374.1| topoisomerase I [Ophiorrhiza liukiuensis]            1096   0.0  
ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [N...  1064   0.0  
ref|XP_023553297.1| DNA topoisomerase 1 beta-like [Cucurbita pep...  1060   0.0  
ref|XP_023007686.1| DNA topoisomerase 1 beta-like [Cucurbita max...  1054   0.0  
ref|XP_022932254.1| DNA topoisomerase 1 beta-like [Cucurbita mos...  1051   0.0  
ref|XP_023748033.1| DNA topoisomerase 1 alpha-like [Lactuca sati...  1036   0.0  
gb|PON81509.1| DNA topoisomerase I [Trema orientalis]                1029   0.0  

>ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum]
 ref|XP_020553411.1| DNA topoisomerase 1 [Sesamum indicum]
          Length = 893

 Score = 1427 bits (3695), Expect = 0.0
 Identities = 719/893 (80%), Positives = 754/893 (84%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MA+E+C+ QKL         PLVFKR            E K+ LSQKHDR L RP+PDVR
Sbjct: 1    MAIESCNDQKLMDDMDEDDEPLVFKRSSSSAKQSQGNSETKRPLSQKHDRPLNRPSPDVR 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
             QNGQST+++KSKTVP S+TPP++ PL SPKSSN+S KALA++  EVD +PSTSVTD+SN
Sbjct: 61   PQNGQSTMIQKSKTVPSSRTPPLRSPLASPKSSNSSAKALAVRPREVDPRPSTSVTDESN 120

Query: 621  SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 800
            SVKHQIKDEK ST+ATG  NEDS+DSEDDKPLSARLPAGLSKVNSNHA K PN  T  QK
Sbjct: 121  SVKHQIKDEKPSTKATGGLNEDSEDSEDDKPLSARLPAGLSKVNSNHANKGPNALTSVQK 180

Query: 801  RRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTERKPSTN 980
             +IP PEDSDDEIPLSSKFQVKSNAGES NR S SGERKPLL+KYDQNGSAS ERKPST 
Sbjct: 181  TKIPKPEDSDDEIPLSSKFQVKSNAGESTNRPSISGERKPLLAKYDQNGSASAERKPSTI 240

Query: 981  LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMXXXXX 1160
            LKKRP DGVK+  ESYLKKPKLS+A+TP+K KE SIK EM++EDDEDHVPIS R+     
Sbjct: 241  LKKRPADGVKSAGESYLKKPKLSDASTPNKTKETSIKDEMKDEDDEDHVPISQRIKQQTS 300

Query: 1161 XXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVI 1340
                     N+TKPI                              GEGQKWTTLVHNGVI
Sbjct: 301  SDKKSSSAKNMTKPISASAKKTSKKPKKVMKNSKYSKSSKVPPSSGEGQKWTTLVHNGVI 360

Query: 1341 FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKILGK 1520
            FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVML+TDYMNKPKFKENFMADWRKILGK
Sbjct: 361  FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLDTDYMNKPKFKENFMADWRKILGK 420

Query: 1521 NHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKV 1700
            NH IQSLEHCDFTPIYEWH                            YMWAIVNGVKEKV
Sbjct: 421  NHVIQSLEHCDFTPIYEWHQKEKEKKKQMTAEEKKALKEEKLKQEEKYMWAIVNGVKEKV 480

Query: 1701 GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT 1880
            GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT
Sbjct: 481  GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT 540

Query: 1881 VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSK 2060
            VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYI GIRAAYTKDFT+K
Sbjct: 541  VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNK 600

Query: 2061 DPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDS 2240
            DP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDFLGKDS
Sbjct: 601  DPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDS 660

Query: 2241 IRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNA 2420
            IRYQNEVEVET VFKAIQQFRSGKKG DD+FDRLDTSKLNAHLKELMPGLTAKVFRTYNA
Sbjct: 661  IRYQNEVEVETPVFKAIQQFRSGKKGSDDMFDRLDTSKLNAHLKELMPGLTAKVFRTYNA 720

Query: 2421 SITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELK 2600
            SITLDD LSRET+GGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQM RLNEKIEELK
Sbjct: 721  SITLDDKLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMTRLNEKIEELK 780

Query: 2601 GVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKEDL 2780
            GV+EELQTDLARAKKGKPPLKSADGKPKRNLNPDALE+KIAQT AKIEKMERDKETKEDL
Sbjct: 781  GVLEELQTDLARAKKGKPPLKSADGKPKRNLNPDALEKKIAQTNAKIEKMERDKETKEDL 840

Query: 2781 KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 841  KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPSFRF 893


>gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]
          Length = 895

 Score = 1376 bits (3562), Expect = 0.0
 Identities = 696/895 (77%), Positives = 742/895 (82%), Gaps = 2/895 (0%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEACDKQKL         PLVFKR            E KKSLSQKHDRQLG+PAPD+R
Sbjct: 1    MAVEACDKQKLMDDMDDDDEPLVFKRSSSAAKQNQSVSETKKSLSQKHDRQLGKPAPDIR 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
            SQNGQST+V+KSK    SKTPPV+ PL SPKSSN+S K+ A+KS  V+SKPSTSV D+SN
Sbjct: 61   SQNGQSTMVEKSKMASSSKTPPVRSPLASPKSSNSSAKSFAVKSSGVNSKPSTSVADESN 120

Query: 621  SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 800
            SVK Q +D++  TEAT E NE+S+DS+DDKPL+ARL AG SK+NSNHA K PN S L QK
Sbjct: 121  SVKQQSRDKRPLTEATAELNEESEDSDDDKPLTARLSAGFSKINSNHANKGPNASILGQK 180

Query: 801  RRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTERKPSTN 980
             +IP  EDSDDEIPLSSKFQVKSNAG+S  +S  SG RKPL +K D NG  S ERKPS++
Sbjct: 181  PKIPKSEDSDDEIPLSSKFQVKSNAGDSSKKSPTSGGRKPLSAKCDHNGLGSPERKPSSS 240

Query: 981  --LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMXXX 1154
              LKKRP DGVK+  ES+LK+PKLS+A+  +KNKE SIKAE++EEDDEDHVPIS RM   
Sbjct: 241  TILKKRPADGVKSAGESHLKRPKLSDASASNKNKETSIKAELKEEDDEDHVPISARMKKS 300

Query: 1155 XXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQKWTTLVHNG 1334
                       NITKP+                              GEGQKWTTLVHNG
Sbjct: 301  TSSDKKPSSSKNITKPVSSLSKKINKKSKKVTKKSEYSKSSKVPPGSGEGQKWTTLVHNG 360

Query: 1335 VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKIL 1514
            VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATM+AVML+TDYMNKPKFKENFM DWRKIL
Sbjct: 361  VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMNKPKFKENFMDDWRKIL 420

Query: 1515 GKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVNGVKE 1694
            GKNH IQSLEHC+FTPIYEWH                            YMWAIV+GVKE
Sbjct: 421  GKNHVIQSLEHCNFTPIYEWHQKEKEKKKQMSADEKKALKEEKLKQEDKYMWAIVDGVKE 480

Query: 1695 KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHD 1874
            KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDI INIGKDAPIPECPIPGESWKE+RHD
Sbjct: 481  KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDIVINIGKDAPIPECPIPGESWKEVRHD 540

Query: 1875 NTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFT 2054
            NTVTWLAYWNDPINPKEFKYVFLAASS LKGQSDKEKYEKARLLKDYI  IRA YTK+FT
Sbjct: 541  NTVTWLAYWNDPINPKEFKYVFLAASSALKGQSDKEKYEKARLLKDYIQDIRATYTKNFT 600

Query: 2055 SKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGK 2234
            SKDP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDFLGK
Sbjct: 601  SKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGK 660

Query: 2235 DSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTY 2414
            DSIRYQNEVEVETAVF+AIQ+FR GKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVFRTY
Sbjct: 661  DSIRYQNEVEVETAVFRAIQEFRRGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTY 720

Query: 2415 NASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE 2594
            NASITLDDMLSRET+GGDVAEKVVVYQ ANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE
Sbjct: 721  NASITLDDMLSRETKGGDVAEKVVVYQLANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE 780

Query: 2595 LKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE 2774
            LKGV+EELQTDLARAKKGKPPLK+ADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE
Sbjct: 781  LKGVLEELQTDLARAKKGKPPLKNADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE 840

Query: 2775 DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 841  DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPSFRF 895


>ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythranthe guttata]
          Length = 894

 Score = 1209 bits (3127), Expect = 0.0
 Identities = 634/909 (69%), Positives = 699/909 (76%), Gaps = 16/909 (1%)
 Frame = +3

Query: 261  MAVEAC-DKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDV 437
            M VEA  DK  +         PLVFKR            E K S SQKH+ Q+GR + DV
Sbjct: 1    MTVEAREDKNMMEDMDDDDDAPLVFKRSGSSSKPKQSEPENKLS-SQKHNAQVGRQSSDV 59

Query: 438  RSQNGQSTIVKKSKTVPLSKT--PPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTD 611
            R QNG +   +K K+V  S +  PPV+  L SPKSSN+S KA  +KSP+V+SKPSTSV  
Sbjct: 60   RPQNGTNANAQKGKSVTSSSSMKPPVRPSLVSPKSSNSSVKASPIKSPDVNSKPSTSVVG 119

Query: 612  KSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTL 791
            +S SVK  IK+E            +S+DSEDDKPLSAR  AG SK N N   K PN   L
Sbjct: 120  ESKSVKSTIKEE------------NSEDSEDDKPLSARQVAGSSKNNPNQTNK-PNAPNL 166

Query: 792  SQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLS-KYDQNGSASTERK 968
             +K  +P PEDSDDEIPLS+KFQ KSNAG S +RSS+S E+KPL++ K D+NGSASTERK
Sbjct: 167  GKKSNVPKPEDSDDEIPLSAKFQGKSNAGASTSRSSHSEEKKPLVARKPDKNGSASTERK 226

Query: 969  PSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMX 1148
            PST L+KRP DGV +  +S LKKPKLS++    K+K+AS+K E++ EDDED VP+S R  
Sbjct: 227  PSTVLQKRPADGVASAGQSNLKKPKLSDSLAS-KDKKASVKPEVKAEDDEDLVPLSQRTK 285

Query: 1149 XXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------- 1295
                          +TK                                           
Sbjct: 286  KSTTPEKKPSPALKVTKSTASISKKTNNKTNSKTNSKTNKKSKQVSKDNNYSKSSKLPPS 345

Query: 1296 -GEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKP 1472
             G+GQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPV+LTPEQEEVATMYAVMLETDY+ KP
Sbjct: 346  SGDGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVNLTPEQEEVATMYAVMLETDYLKKP 405

Query: 1473 KFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXX 1652
            +FKENFM DW+KILGKNH IQSLEHCDFTPIYEWH                         
Sbjct: 406  QFKENFMTDWKKILGKNHVIQSLEHCDFTPIYEWHQREKEKKKQMSTDEKKALKEEKLKL 465

Query: 1653 XXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPE 1832
               YMWA+++GV+EKVGNFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIG+DAPIPE
Sbjct: 466  EEKYMWAVLDGVREKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGRDAPIPE 525

Query: 1833 CPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKD 2012
            CPI GE WKEIRHDNTVTWLAYW+DPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLKD
Sbjct: 526  CPIEGERWKEIRHDNTVTWLAYWSDPINGKEFKYVFLAASSTLKGQSDKEKYEKARLLKD 585

Query: 2013 YILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 2192
            YI GIR AYT+DF SKDPAK+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE
Sbjct: 586  YIQGIRTAYTRDFVSKDPAKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 645

Query: 2193 PVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLK 2372
            PVPPN LKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFD+LDTSKLNAHLK
Sbjct: 646  PVPPNILKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDKLDTSKLNAHLK 705

Query: 2373 ELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKS 2552
            ELMPGLTAKVFRTYNASITLDD LSRET+GGD+AEKVV Y HANKEVAIICNHQRTVSK+
Sbjct: 706  ELMPGLTAKVFRTYNASITLDDKLSRETKGGDLAEKVVTYNHANKEVAIICNHQRTVSKT 765

Query: 2553 HSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTT 2732
            HSAQMLRL+EKI+ELKGV+EEL+TDL RA+KGKP L  ADGK KRN+NP+ALERKIAQTT
Sbjct: 766  HSAQMLRLDEKIDELKGVLEELKTDLGRARKGKPLLAGADGKAKRNINPEALERKIAQTT 825

Query: 2733 AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWA 2912
            AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSLL KF+WA
Sbjct: 826  AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKMFNKSLLEKFSWA 885

Query: 2913 MDVDPGFRF 2939
            MDVDP FRF
Sbjct: 886  MDVDPSFRF 894


>gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythranthe guttata]
          Length = 883

 Score = 1209 bits (3127), Expect = 0.0
 Identities = 633/898 (70%), Positives = 697/898 (77%), Gaps = 5/898 (0%)
 Frame = +3

Query: 261  MAVEAC-DKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDV 437
            M VEA  DK  +         PLVFKR            E K S SQKH+ Q+GR + DV
Sbjct: 1    MTVEAREDKNMMEDMDDDDDAPLVFKRSGSSSKPKQSEPENKLS-SQKHNAQVGRQSSDV 59

Query: 438  RSQNGQSTIVKKSKTVPLSKT--PPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTD 611
            R QNG +   +K K+V  S +  PPV+  L SPKSSN+S KA  +KSP+V+SKPSTSV  
Sbjct: 60   RPQNGTNANAQKGKSVTSSSSMKPPVRPSLVSPKSSNSSVKASPIKSPDVNSKPSTSVVG 119

Query: 612  KSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTL 791
            +S SVK  IK+E            +S+DSEDDKPLSAR  AG SK N N   K PN   L
Sbjct: 120  ESKSVKSTIKEE------------NSEDSEDDKPLSARQVAGSSKNNPNQTNK-PNAPNL 166

Query: 792  SQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLS-KYDQNGSASTERK 968
             +K  +P PEDSDDEIPLS+KFQ KSNAG S +RSS+S E+KPL++ K D+NGSASTERK
Sbjct: 167  GKKSNVPKPEDSDDEIPLSAKFQGKSNAGASTSRSSHSEEKKPLVARKPDKNGSASTERK 226

Query: 969  PSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMX 1148
            PST L+KRP DGV +  +S LKKPKLS++    K+K+AS+K E++ EDDED VP+S R  
Sbjct: 227  PSTVLQKRPADGVASAGQSNLKKPKLSDSLAS-KDKKASVKPEVKAEDDEDLVPLSQRTK 285

Query: 1149 XXXXXXXXXXXXXNIT-KPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQKWTTLV 1325
                             K                                G+GQKWTTLV
Sbjct: 286  KSTTPEKKPSPALKTNNKTNSKTNSKTNKKSKQVSKDNNYSKSSKLPPSSGDGQKWTTLV 345

Query: 1326 HNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWR 1505
            HNGVIFPPPYKPHGVKMLYKGKPV+LTPEQEEVATMYAVMLETDY+ KP+FKENFM DW+
Sbjct: 346  HNGVIFPPPYKPHGVKMLYKGKPVNLTPEQEEVATMYAVMLETDYLKKPQFKENFMTDWK 405

Query: 1506 KILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVNG 1685
            KILGKNH IQSLEHCDFTPIYEWH                            YMWA+++G
Sbjct: 406  KILGKNHVIQSLEHCDFTPIYEWHQREKEKKKQMSTDEKKALKEEKLKLEEKYMWAVLDG 465

Query: 1686 VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEI 1865
            V+EKVGNFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIG+DAPIPECPI GE WKEI
Sbjct: 466  VREKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGRDAPIPECPIEGERWKEI 525

Query: 1866 RHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTK 2045
            RHDNTVTWLAYW+DPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLKDYI GIR AYT+
Sbjct: 526  RHDNTVTWLAYWSDPINGKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIQGIRTAYTR 585

Query: 2046 DFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDF 2225
            DF SKDPAK+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDF
Sbjct: 586  DFVSKDPAKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDF 645

Query: 2226 LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVF 2405
            LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVF
Sbjct: 646  LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVF 705

Query: 2406 RTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEK 2585
            RTYNASITLDD LSRET+GGD+AEKVV Y HANKEVAIICNHQRTVSK+HSAQMLRL+EK
Sbjct: 706  RTYNASITLDDKLSRETKGGDLAEKVVTYNHANKEVAIICNHQRTVSKTHSAQMLRLDEK 765

Query: 2586 IEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKE 2765
            I+ELKGV+EEL+TDL RA+KGKP L  ADGK KRN+NP+ALERKIAQTTAKIEKMERDKE
Sbjct: 766  IDELKGVLEELKTDLGRARKGKPLLAGADGKAKRNINPEALERKIAQTTAKIEKMERDKE 825

Query: 2766 TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSLL KF+WAMDVDP FRF
Sbjct: 826  TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKMFNKSLLEKFSWAMDVDPSFRF 883


>gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]
          Length = 870

 Score = 1194 bits (3089), Expect = 0.0
 Identities = 628/898 (69%), Positives = 696/898 (77%), Gaps = 5/898 (0%)
 Frame = +3

Query: 261  MAVEACDKQK-LXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDV 437
            MAV+A D++K L         PLVFKR                S S KH+ + G+     
Sbjct: 1    MAVDAFDEKKSLDDVDDDDDQPLVFKR---------------SSSSSKHNLKNGQ----- 40

Query: 438  RSQNGQSTIVKKSKTVP-LSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDK 614
            +S+NG+S+ V+K   VP  SK PP+K PLTSPKSS+++ K   +KS   +SK   S TDK
Sbjct: 41   QSRNGESSSVQKGTLVPPSSKAPPIKSPLTSPKSSSSAAKESPVKSSAANSKRPMSETDK 100

Query: 615  SNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 794
             NSVK Q+K+E+   +A  E + D +DS DD PLSARL A  SK NSNHAK+ PN   L 
Sbjct: 101  PNSVKQQVKEEQPFAQAADETH-DLEDSGDDTPLSARLLASSSKGNSNHAKQRPNALALV 159

Query: 795  QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE---R 965
            QK  IP  EDSDDE+PLSSKFQVKS+   S +RS+NS ERKP+L+K DQNGS+ST+   +
Sbjct: 160  QKNSIPKKEDSDDEMPLSSKFQVKSDVRVSTSRSNNSDERKPILNKADQNGSSSTDHSQK 219

Query: 966  KPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRM 1145
            KPS  +K RP + VK    S  KKPKLS A++      A  +A+ + EDD+DHVPIS RM
Sbjct: 220  KPSIAMK-RPPNPVKVECRSPEKKPKLSNASS------AIPRAKPKAEDDDDHVPISQRM 272

Query: 1146 XXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQKWTTLV 1325
                           +TK +                              GEGQKWTTLV
Sbjct: 273  KNQSSSEKKSLSVKKVTKSVSAVATKTNKISKKIVKNTKYSISSKVLPNSGEGQKWTTLV 332

Query: 1326 HNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWR 1505
            HNGVIFPPPYKPHGVKMLYKGKPV LTPEQEEVATM+AVML+TDYMNKPKFKENFM DWR
Sbjct: 333  HNGVIFPPPYKPHGVKMLYKGKPVVLTPEQEEVATMFAVMLDTDYMNKPKFKENFMTDWR 392

Query: 1506 KILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVNG 1685
            KILGKNHTIQ LE CDFTPIYEWH                            YMWAI++G
Sbjct: 393  KILGKNHTIQKLEDCDFTPIYEWHQKEKEKKKQMSTEEKKALKEEKLKQEEKYMWAILDG 452

Query: 1686 VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEI 1865
            VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI+PSDI INIGKDA IPECPIPGESWKEI
Sbjct: 453  VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIQPSDIIINIGKDAAIPECPIPGESWKEI 512

Query: 1866 RHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTK 2045
            RHDNTVTWLAYWNDPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLK+YI GIRAAYTK
Sbjct: 513  RHDNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKNYIQGIRAAYTK 572

Query: 2046 DFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDF 2225
            DF+SKDP ++QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDF
Sbjct: 573  DFSSKDPTRKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDF 632

Query: 2226 LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVF 2405
            LGKDSIRYQNEV VETAVFKAIQQFR+GKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVF
Sbjct: 633  LGKDSIRYQNEVVVETAVFKAIQQFRNGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVF 692

Query: 2406 RTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEK 2585
            RTYNASITLDDML+RET+GG+VAEK+VVYQHANKEVAIICNHQRTVSKSHSAQMLRL+EK
Sbjct: 693  RTYNASITLDDMLNRETKGGEVAEKIVVYQHANKEVAIICNHQRTVSKSHSAQMLRLDEK 752

Query: 2586 IEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKE 2765
            I+ELKG +EEL+ DL+RAKKGKPPLKSADGKPKRN+NP+ALE+KIAQT AKIEKMERDKE
Sbjct: 753  IDELKGAMEELREDLSRAKKGKPPLKSADGKPKRNMNPEALEKKIAQTAAKIEKMERDKE 812

Query: 2766 TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            TKEDLKT+ALGTS+INYLDPRITVAWCKR EVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 813  TKEDLKTIALGTSRINYLDPRITVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPEFRF 870


>emb|CDP13767.1| unnamed protein product [Coffea canephora]
          Length = 926

 Score = 1162 bits (3006), Expect = 0.0
 Identities = 609/927 (65%), Positives = 678/927 (73%), Gaps = 34/927 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         PLVFKR            E KK  SQK D Q  R + DV 
Sbjct: 1    MAVEACSTPSLLEDSDEENAPLVFKRTSSSSKQNLSNSETKKLSSQKRDGQSSRNSSDVC 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NGQS+  +  + V  +KTPP K PL+SPK++N S K   + SP  +SKPS+S +    
Sbjct: 61   PVNGQSSSAQTGRVVSSAKTPPAKSPLSSPKAANISAKTSLITSPGANSKPSSSTSATVK 120

Query: 621  SVKHQ-----IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTS 785
             +++      +K  K S  A  + +   +DS+DDKPL A+LP+G S+   NH  K  + S
Sbjct: 121  QLENHKNSPVVKLPKRSVSAA-KLSSTPEDSDDDKPLMAKLPSGSSRAIVNHGNKDGDGS 179

Query: 786  TLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER 965
            +L QK  +   EDSDDE PLSS++  KSN  ESI++S N GE+K L +K  QNGSA  + 
Sbjct: 180  SLVQKPGVEEDEDSDDEKPLSSRYLSKSNLVESISKSRNPGEKKSLPAKVQQNGSALRDG 239

Query: 966  ---KPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDED----- 1121
               K S+   KRP   VK++DES +KKPKLS        K+A++KAE + EDD+D     
Sbjct: 240  PVGKSSSLSNKRPPGEVKSSDESAVKKPKLSGPTAAINKKQATLKAEPKVEDDDDDILIS 299

Query: 1122 ---------------------HVPISHRMXXXXXXXXXXXXXXNITKPIXXXXXXXXXXX 1238
                                 HVPI+ RM               + K +           
Sbjct: 300  QTAKKMFASASKPKSNDDDDDHVPITQRMKKSTTSVSQSSSVKKVAKVVSSSLKKTNKKS 359

Query: 1239 XXXXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQE 1418
                               GEGQKWTTLVHNGVIFPPPYKPHGVKMLYKG+PV LTPEQE
Sbjct: 360  KKIIKKSKYSKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGQPVTLTPEQE 419

Query: 1419 EVATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXX 1598
            EVATM+AVML+TDYM+KP+FKENF +DW++ILGKNHTIQ+LE CDF PIYEWH       
Sbjct: 420  EVATMFAVMLDTDYMSKPQFKENFFSDWKRILGKNHTIQNLEDCDFRPIYEWHQKEKEKK 479

Query: 1599 XXXXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 1778
                                 YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR
Sbjct: 480  KQMTTEEKKSLKEEKLKQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 539

Query: 1779 IRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASST 1958
            IRP DITINIGKDAPIPECPIPGE WKEIRHDNTVTWLA+WNDPINPKEFKYVFLAASST
Sbjct: 540  IRPRDITINIGKDAPIPECPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASST 599

Query: 1959 LKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKD 2138
            LKGQSDKEKYEKARLLKDYI GIR AYTKDF SKDP K+QIAVATYLIDKLALRAGNEKD
Sbjct: 600  LKGQSDKEKYEKARLLKDYIHGIRTAYTKDFASKDPMKKQIAVATYLIDKLALRAGNEKD 659

Query: 2139 DDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKG 2318
            DDEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEVEVE+ VFKAIQQFRSGK+G
Sbjct: 660  DDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVESGVFKAIQQFRSGKEG 719

Query: 2319 GDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQH 2498
             +DLFD+LDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSR+T GGDV EKVVVYQ 
Sbjct: 720  SEDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRKTNGGDVLEKVVVYQQ 779

Query: 2499 ANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGK 2678
            ANKEVAIICNHQRTVSK+HSAQM RLNEKIEELKGV+EEL+TDL+RAKKGKPPLK+ADGK
Sbjct: 780  ANKEVAIICNHQRTVSKTHSAQMSRLNEKIEELKGVLEELKTDLSRAKKGKPPLKNADGK 839

Query: 2679 PKRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE 2858
            PKRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE
Sbjct: 840  PKRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE 899

Query: 2859 VPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            VPIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  VPIEKIFNKSLLAKFAWSMDVDPSFRF 926


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1141 bits (2952), Expect = 0.0
 Identities = 600/903 (66%), Positives = 675/903 (74%), Gaps = 10/903 (1%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAV+AC K+K+         PLVFKR            + KKS SQ++D Q GR   DV 
Sbjct: 1    MAVQACVKEKMMDEIDEDDEPLVFKRSNATSKHNQLNSDTKKS-SQRYDGQSGRQVSDVP 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
            S NGQS+   K KT  LS    +K P+ SPK+S +  KA  + +P+V S  S+    K +
Sbjct: 60   STNGQSSSASKGKTTKLSS---MKSPIASPKASTSFAKASPVANPKVSS--SSDDRSKHS 114

Query: 621  SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 800
            S ++ I   K   E      E   DSED+KPLSARL  GL+K +SN+A K    S+ +  
Sbjct: 115  SKQNTINVVKEEKELVNPAAEPYGDSEDEKPLSARLFTGLTKGSSNNANKGLINSSPAS- 173

Query: 801  RRIPIP--------EDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSAS 956
              +P+P        +DSDDEIPLSSKF++K+NAG S  +S  S + K L+S + QNGS +
Sbjct: 174  --LPVPKPEINRYSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSIN 231

Query: 957  TERKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREED-DEDHVPI 1133
               K S  + KRP   VK++ +S +KKPKLS+A+TP  NK+AS KAE + +D D+DHVPI
Sbjct: 232  RGSKSSIKVNKRPLGEVKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDDHVPI 291

Query: 1134 SHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQK 1310
            S RM                +TK +                              GEG+K
Sbjct: 292  SQRMKKSATSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSGEGKK 351

Query: 1311 WTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENF 1490
            W TLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATM+AVML+TDYM K KFKENF
Sbjct: 352  WNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKFKENF 411

Query: 1491 MADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMW 1670
            M DWRKILGKNH IQ+LE CDFTPIYEWH                            YMW
Sbjct: 412  MDDWRKILGKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEEKYMW 471

Query: 1671 AIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGE 1850
            AIVNGVKEKVGNFRVEPP LFRGRGEHPKMGKLKK IRPSDITINIGKDAPIPECPIPGE
Sbjct: 472  AIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECPIPGE 531

Query: 1851 SWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIR 2030
            SWKEIRHDNTVTWLA+WNDPI P+EFKYVFLAASS+LKGQSDKEKYEKARLLKD+I GIR
Sbjct: 532  SWKEIRHDNTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFIQGIR 591

Query: 2031 AAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNT 2210
            AAYTKDF SKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PP+ 
Sbjct: 592  AAYTKDFASKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPSI 651

Query: 2211 LKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGL 2390
            LKFDFLGKDSIRYQNEVEVE  V+KAIQQFR+GK+GGDDLFD+LDTSKLNAHLK LMPGL
Sbjct: 652  LKFDFLGKDSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGLMPGL 711

Query: 2391 TAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQML 2570
            TAKVFRTYNASITLD+MLSRET+GG+VAEK+VVYQHANKEVAIICNHQRTVSKSH AQM 
Sbjct: 712  TAKVFRTYNASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHPAQMT 771

Query: 2571 RLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKM 2750
            RLN KI+ELKG+++ LQTDLARAKKGKPPL+ ADGKPKRNL P+ALERKI QT AKIEKM
Sbjct: 772  RLNGKIDELKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAKIEKM 831

Query: 2751 ERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPG 2930
            ERDKETKE LKT+ALGTSKI+YLDPRITVAWCKRHEVPIEK+FNKSLLAKFAW+MDVDP 
Sbjct: 832  ERDKETKEGLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMDVDPS 891

Query: 2931 FRF 2939
            FRF
Sbjct: 892  FRF 894


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1129 bits (2921), Expect = 0.0
 Identities = 600/919 (65%), Positives = 662/919 (72%), Gaps = 26/919 (2%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P+VFKR            E +K  SQK D Q  R   D R
Sbjct: 1    MAVEACPTPNLRDDMDEDDEPIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSR 60

Query: 441  SQNGQSTIVKKSKTVPLSK---------TPP-----VKYPLTSPKSSNTSGKALALKSPE 578
              NG+S+  +K + VP SK         +PP     VK PL+SPK S +S K   + S  
Sbjct: 61   PANGESSSGQKGRMVPPSKPSQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLP 120

Query: 579  VDSKPSTSVTDKSNSVKHQ-----IKDEKLST-EATGEPNEDSQDSEDDKPLSARLPAGL 740
             +SKPSTS + K            IK+EK S          DS+DS+D KPLS R  A  
Sbjct: 121  ANSKPSTSGSFKVKPFNQHKSATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLRHSASS 180

Query: 741  SKVNSNHAKKSPNTSTLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKP 920
            SK N+N   K  +          P  EDSDDE PLSS+F +KS  GES +++ +S + KP
Sbjct: 181  SKGNTNQVSKEGSK---------PENEDSDDEKPLSSRFPLKSGVGESTSKAYDSDDDKP 231

Query: 921  LLSKYDQNGSASTERKP---STNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIK 1091
            L  K   NGSA  + +P   S    KRP   +K++++  +KKPKLS    P   K+AS+K
Sbjct: 232  LAKKLQHNGSAMRDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPTNKKQASVK 291

Query: 1092 AEMR-EEDDEDHVPISHRMXXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXX 1268
             E + E+DD+DH+PIS R+                TK +                     
Sbjct: 292  PEPKAEDDDDDHLPISQRIKKSPTSESKSSSIKKTTKVVSSSVKKVNKKSKKVMKNSKYS 351

Query: 1269 XXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVML 1448
                     GEG+KW  L HNGVIFPPPYKPHG+KMLYKGKPVDLTPEQEEVATM+AVML
Sbjct: 352  KSSKVPPSSGEGKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEEVATMFAVML 411

Query: 1449 ETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXX 1628
            +TDYMNKP+FKENF  DW+KILGKNH IQ+LE+CDF+PIYEWH                 
Sbjct: 412  DTDYMNKPRFKENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKKQMTTEEKKA 471

Query: 1629 XXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINI 1808
                       YMWA V+G KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINI
Sbjct: 472  LKEEKLKQEEKYMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINI 531

Query: 1809 GKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKY 1988
            GKDAPIPECP+PGE WKE+RHDNTVTWLA+WNDPINPKEFKYVFLAASSTLKG SDKEKY
Sbjct: 532  GKDAPIPECPVPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTLKGLSDKEKY 591

Query: 1989 EKARLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCC 2168
            EKARLLKDYI GIRAAYTKDF SKDP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCC
Sbjct: 592  EKARLLKDYIQGIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCC 651

Query: 2169 TLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDT 2348
            TLKVENVEPVPPN LKFDFLGKDSIRYQNEVEVE AVFKAIQQFRSGK+G  DLFD LDT
Sbjct: 652  TLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGSSDLFDLLDT 711

Query: 2349 SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICN 2528
            SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRET+GGDVAEKVVVYQHANKEVAIICN
Sbjct: 712  SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHANKEVAIICN 771

Query: 2529 HQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKS--ADGKPKRNLNPD 2702
            HQRTVSKSHSAQMLRLNEKIEELK VVEEL++DL+R KKGKPPLKS  ADGKPKRNLNP+
Sbjct: 772  HQRTVSKSHSAQMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADGKPKRNLNPE 831

Query: 2703 ALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN 2882
            +LE+KIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN
Sbjct: 832  SLEKKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN 891

Query: 2883 KSLLAKFAWAMDVDPGFRF 2939
            KSLLAKF WAMDVDP FRF
Sbjct: 892  KSLLAKFTWAMDVDPSFRF 910


>ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea var. sylvestris]
          Length = 771

 Score = 1127 bits (2915), Expect = 0.0
 Identities = 573/772 (74%), Positives = 621/772 (80%), Gaps = 4/772 (0%)
 Frame = +3

Query: 636  IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQKRRIPI 815
            +K EK ST    EPN DS+DSEDDKPLSARL   LSK NSN+A K  N S L QK  IP 
Sbjct: 1    MKVEKPSTGPAAEPN-DSEDSEDDKPLSARLSTSLSKNNSNNASKGSNASRLGQKPSIPK 59

Query: 816  PEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSA---STERKPSTNLK 986
             EDSDDE PLS K Q+KSN G S  +S +S E+  L +K +QNGS    S  +KPST L 
Sbjct: 60   QEDSDDEKPLSHKVQLKSNVGSSTRKSYDSDEKMHLAAKNNQNGSPLQDSGSKKPSTILS 119

Query: 987  KRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMR-EEDDEDHVPISHRMXXXXXX 1163
            KRP   V +  +S +KK K+S A+    NK+AS+KAE + E+DD+DH+PIS RM      
Sbjct: 120  KRPASEVNSAGQSSIKKHKISNASVLINNKQASVKAEPKVEDDDDDHIPISQRMKKPAAS 179

Query: 1164 XXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIF 1343
                     +TK +                              GEGQKWTTL+HNGVIF
Sbjct: 180  DKKISAIKKVTKVVSSSLNKTNKNSKKVVKKSQYSKSSKVPPGSGEGQKWTTLIHNGVIF 239

Query: 1344 PPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKILGKN 1523
            PPPY+ HGVKMLYKGKPV+LTPEQEEVATM+AVML+T+YMNKPKFKENFM+DWRKILGKN
Sbjct: 240  PPPYQSHGVKMLYKGKPVNLTPEQEEVATMFAVMLDTEYMNKPKFKENFMSDWRKILGKN 299

Query: 1524 HTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVG 1703
            H IQ+LE CDFTPIYEWH                            YMWAIV+GVKEKVG
Sbjct: 300  HIIQNLEDCDFTPIYEWHQKEKEKKKQMTTEEKKVIKEEKMKQEEKYMWAIVDGVKEKVG 359

Query: 1704 NFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV 1883
            NFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV
Sbjct: 360  NFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV 419

Query: 1884 TWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSKD 2063
            TWLA+WNDPINPKE KYVFLAASSTLKGQSDKEKYEK+RLLKDYI GIRA+YTKDF SKD
Sbjct: 420  TWLAFWNDPINPKEIKYVFLAASSTLKGQSDKEKYEKSRLLKDYIQGIRASYTKDFASKD 479

Query: 2064 PAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSI 2243
            P K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP+PPN LKFDFLGKDSI
Sbjct: 480  PTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPMPPNILKFDFLGKDSI 539

Query: 2244 RYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNAS 2423
            RYQNEVEVE+AVFKAIQQFRSGKK  DDLFD+LDTSKLNAHLKELMPGLTAKVFRTYNAS
Sbjct: 540  RYQNEVEVESAVFKAIQQFRSGKKAADDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNAS 599

Query: 2424 ITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKG 2603
            ITLD+MLS++T+ GDVAE +VVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELK 
Sbjct: 600  ITLDEMLSKDTKAGDVAENIVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKV 659

Query: 2604 VVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKEDLK 2783
            V+EEL TDL RAKKGKPPLKSADGK KRNLNP+ALE+KIAQT AKIEKMERDKETKEDLK
Sbjct: 660  VLEELTTDLVRAKKGKPPLKSADGKTKRNLNPEALEKKIAQTNAKIEKMERDKETKEDLK 719

Query: 2784 TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKF WAMDVDP FRF
Sbjct: 720  TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFTWAMDVDPSFRF 771


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1122 bits (2901), Expect = 0.0
 Identities = 595/926 (64%), Positives = 664/926 (71%), Gaps = 33/926 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P++FKR            E KK   QK D Q      DVR
Sbjct: 1    MAVEACTTN-LMEDMEDDGGPVIFKRSKPAPKQNQANSEKKKLSLQKQDGQSAVHTSDVR 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NG+S+  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 621  SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 788
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 179

Query: 789  LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 962
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGSAS +  
Sbjct: 180  SIRRPVIEESEDSEDEKPLSCRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSASRDGP 239

Query: 963  -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 1118
              K ++   KRP    K+ ++S +KKPKLS   T   NK+A++K E + EDD+       
Sbjct: 240  MNKSASLSNKRPPGEAKSLNQSSVKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 299

Query: 1119 -----------------DHVPISHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXX 1244
                             D+VPIS R+                 +  +             
Sbjct: 300  RGKKSQSATSKPKDDDDDNVPISQRIKKSPASVSKSSVPVKKASTVVSSSLKKVNKNSKK 359

Query: 1245 XXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1424
                             GEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 360  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 419

Query: 1425 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1604
            ATM+AVML+TDYMNKP FKENF +DW+KILGKNH IQ+LE CDF PIYEWH         
Sbjct: 420  ATMFAVMLDTDYMNKPCFKENFFSDWKKILGKNHMIQNLEDCDFGPIYEWHQQEKEKKKQ 479

Query: 1605 XXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1784
                               YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 480  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 539

Query: 1785 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1964
            P DITINIGKDAPIPECPIPGE WKE+R+ NTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 540  PRDITINIGKDAPIPECPIPGERWKEVRNGNTVTWLAYWNDPINQKEFKYVFLAASSTLK 599

Query: 1965 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 2141
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 600  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 659

Query: 2142 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 2321
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 660  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGS 719

Query: 2322 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 2501
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG+VAE VVVYQHA
Sbjct: 720  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAENVVVYQHA 779

Query: 2502 NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 2681
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKPP K ADGK 
Sbjct: 780  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPPTKGADGKA 839

Query: 2682 KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 2861
            KRNLNP+ALERKI QT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 840  KRNLNPEALERKITQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 899

Query: 2862 PIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            PIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  PIEKIFNKSLLAKFAWSMDVDPSFRF 925


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1119 bits (2895), Expect = 0.0
 Identities = 591/928 (63%), Positives = 667/928 (71%), Gaps = 35/928 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P++FKR            E KK   QKH  Q      DVR
Sbjct: 1    MAVEACTPN-LMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NG+S+  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 621  SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 788
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 179

Query: 789  LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 962
              ++R I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGS S +  
Sbjct: 180  SIRRRVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSVSRDGP 239

Query: 963  -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMR------------ 1103
              K ++   KRP   VK+ ++S +KKPKLS   T   NK+A++K E              
Sbjct: 240  MNKSASLSNKRPPGEVKSLNQSSVKKPKLSSPITSISNKQAAVKPEPEPKAEDDDDNIPL 299

Query: 1104 --------------EEDDEDHVPISHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXX 1238
                          +++D+D+VPI+ R+                 +  +           
Sbjct: 300  SQRGKKSQAATSKPKDEDDDNVPIAQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKNS 359

Query: 1239 XXXXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQE 1418
                               GEGQKWTTLVHNGVIFPPPY PHGVKMLYK +P+ LTPEQE
Sbjct: 360  KKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYNPHGVKMLYKRQPITLTPEQE 419

Query: 1419 EVATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXX 1598
            EVATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH       
Sbjct: 420  EVATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKK 479

Query: 1599 XXXXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 1778
                                 YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR
Sbjct: 480  KQMTTEEKKALKEDKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 539

Query: 1779 IRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASST 1958
            IRP DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLA+W DPIN KEFKYVFLAASST
Sbjct: 540  IRPRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWIDPINQKEFKYVFLAASST 599

Query: 1959 LKGQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEK 2135
            LKGQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEK
Sbjct: 600  LKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEK 659

Query: 2136 DDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKK 2315
            DDDEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+
Sbjct: 660  DDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKE 719

Query: 2316 GGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQ 2495
            G +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG+VAEKVVVYQ
Sbjct: 720  GSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAEKVVVYQ 779

Query: 2496 HANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADG 2675
            HANKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+R KKGKPP K ADG
Sbjct: 780  HANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTAMEELKTDLSRVKKGKPPSKGADG 839

Query: 2676 KPKRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 2855
            KPKRNLNP+ALERK+AQ  AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH
Sbjct: 840  KPKRNLNPEALERKMAQINAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 899

Query: 2856 EVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            EVPIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  EVPIEKIFNKSLLAKFAWSMDVDPSFRF 927


>dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]
          Length = 920

 Score = 1115 bits (2883), Expect = 0.0
 Identities = 590/919 (64%), Positives = 661/919 (71%), Gaps = 33/919 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P++FKR            E KK   QKH  Q      DVR
Sbjct: 1    MAVEACTTTNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NG+++  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 61   PANGENSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 120

Query: 621  SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 788
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 180

Query: 789  LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 962
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K   NGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSP 240

Query: 963  -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 1118
              K ++   KRP   VK+ ++S LKKPKLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLSNKRPPGEVKSLNQSSLKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 1119 -----------------DHVPISHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXX 1244
                             D+VPIS R+                 +  +             
Sbjct: 301  RGKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 1245 XXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1424
                             GEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 420

Query: 1425 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1604
            ATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQ 480

Query: 1605 XXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1784
                               YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1785 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1964
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLK 600

Query: 1965 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 2141
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 2142 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 2321
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAI QFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIHQFRSGKEGS 720

Query: 2322 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 2501
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS++T GG+VAEKVVVYQHA
Sbjct: 721  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHA 780

Query: 2502 NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 2681
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKP  K ADGKP
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKP 840

Query: 2682 KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 2861
            KRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 900

Query: 2862 PIEKIFNKSLLAKFAWAMD 2918
            PIEKIFNKSLLAKFAWAM+
Sbjct: 901  PIEKIFNKSLLAKFAWAMN 919


>dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. glabra]
          Length = 920

 Score = 1112 bits (2877), Expect = 0.0
 Identities = 589/919 (64%), Positives = 661/919 (71%), Gaps = 33/919 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P++FKR            E KK   Q+H  Q      DVR
Sbjct: 1    MAVEACTTTNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQRHVGQSAVHTSDVR 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NG+++  +K +  P +KTPPVK PL+SPK+SN+S KA  + S   +SKPS S T  + 
Sbjct: 61   PANGENSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSAGANSKPSASATSTAK 120

Query: 621  SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 788
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 180

Query: 789  LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 962
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K   NGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSP 240

Query: 963  -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 1118
              K ++   KRP   VK+ ++S LKKPKLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLSNKRPPGEVKSLNQSSLKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 1119 -----------------DHVPISHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXX 1244
                             D+VPIS R+                 +  +             
Sbjct: 301  RGKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 1245 XXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1424
                             GEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 420

Query: 1425 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1604
            ATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQ 480

Query: 1605 XXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1784
                               YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1785 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1964
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLK 600

Query: 1965 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 2141
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 2142 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 2321
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGS 720

Query: 2322 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 2501
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS++T GG+VAEKVVVYQHA
Sbjct: 721  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHA 780

Query: 2502 NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 2681
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKP  K ADGKP
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKP 840

Query: 2682 KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 2861
            KRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 900

Query: 2862 PIEKIFNKSLLAKFAWAMD 2918
            PIEKIFNKSLLAKFAWAM+
Sbjct: 901  PIEKIFNKSLLAKFAWAMN 919


>dbj|BAG31374.1| topoisomerase I [Ophiorrhiza liukiuensis]
          Length = 926

 Score = 1096 bits (2834), Expect = 0.0
 Identities = 581/926 (62%), Positives = 658/926 (71%), Gaps = 33/926 (3%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVEAC    L         P++FKR            E KK   QKH  Q      DVR
Sbjct: 1    MAVEACTTPNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 60

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
              NG+S+  +K +  P ++TPPVK PL+SPK+SN+S KA  + SP  +SK S S T  + 
Sbjct: 61   PANGESSSAQKGRIQPSAETPPVKSPLSSPKASNSSAKASPITSPGANSKLSASATSTAK 120

Query: 621  SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 788
               H        E   +    + N +S+ S+D+KPL AR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLIARTSGCSSKGKSNHANKEGGDSC 180

Query: 789  LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 962
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFPSKSSLGESTSKSYDSDEKKPLATKVQQNGSASRDGP 240

Query: 963  -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 1118
              K ++ L KR    VK+ ++S LKK KLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLLNKRAPGEVKSLNQSSLKKAKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 1119 -----------------DHVPISHRMXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXX 1244
                             D+ PIS R+                 +  +             
Sbjct: 301  RGKKSQTAASKPKDEDDDNAPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 1245 XXXXXXXXXXXXXXXXXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1424
                             GEGQKWTTLVHNGV+F PPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVMFLPPYKPHGVKMLYKXQPLTLTPEQEEV 420

Query: 1425 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1604
            ATM+AVML+TDYM KP+FKENF +DW+K+LGKNHT+Q+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMTKPRFKENFFSDWKKMLGKNHTVQNLEDCDFGPIYEWHQQEKEKRKQ 480

Query: 1605 XXXXXXXXXXXXXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1784
                               YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEERLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1785 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1964
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLA+WNDPIN KE KYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWNDPINQKECKYVFLAASSTLK 600

Query: 1965 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 2141
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 2142 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 2321
            DEADTVGCCTLKVENVEPVPPN LK DF+GKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKIDFIGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGC 720

Query: 2322 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 2501
             DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG VAEKV VYQHA
Sbjct: 721  ADLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGKVAEKVGVYQHA 780

Query: 2502 NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 2681
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKPP K ADG+P
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPPAKGADGEP 840

Query: 2682 KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 2861
            KRNLNP++LERKIAQT AKIEKMERDK+TKEDLK VAL TSKI+YLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPESLERKIAQTNAKIEKMERDKKTKEDLKAVALSTSKISYLDPRITVAWCKRHEV 900

Query: 2862 PIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            PIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 901  PIEKIFNKSLLAKFAWSMDVDPSFRF 926


>ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [Nelumbo nucifera]
          Length = 890

 Score = 1064 bits (2751), Expect = 0.0
 Identities = 569/910 (62%), Positives = 646/910 (70%), Gaps = 16/910 (1%)
 Frame = +3

Query: 258  LMAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDV 437
            +MAV+AC K K          P++FKR            E KK  S+K D   G+   D 
Sbjct: 1    MMAVDACIKPKFFDDGDDDEGPIIFKRNNASSKKNQLNKELKKVSSRKRDGSSGKCVSDS 60

Query: 438  RSQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDK- 614
             S NGQ++ ++K K V                    S KA   KS E  SK STS+TDK 
Sbjct: 61   FSPNGQNSSLQKGKAVQ-------------------SSKASTGKSTETSSKASTSITDKL 101

Query: 615  -SNSVKHQIKD--EKLSTEATGEPNEDSQDSEDDKPLSARLP--AGLSKVNSNHAKKSPN 779
              +  K+   D  EK   +   EP  DS DS+DDKPLS RLP  A L K  S+   K  +
Sbjct: 102  KCSPQKNSSIDNMEKQLKKPCNEPYTDSVDSDDDKPLSIRLPTSASLLKCKSSPVSKGLS 161

Query: 780  T----STLSQKRRIP---IPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYD 938
            +    S+ S+K  +      EDS+DE+PLS K ++K + G S  +  +SGE KPL+SK  
Sbjct: 162  SPVASSSQSEKTNVTNKKSSEDSEDELPLSLKLKLKPSVGLSGKKFYDSGEEKPLVSKLQ 221

Query: 939  QNGSASTERKP---STNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREE 1109
            QNGS+  + +P     NL KRP  G  ++ +S +KK K+SE + P K K+ SIK+E +EE
Sbjct: 222  QNGSSKRDNEPLKSPKNLNKRPLSGANSSGQSLVKKLKVSEVSVPTKVKQTSIKSEPKEE 281

Query: 1110 DDEDHVPISHRMXXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1289
            D++D +PIS R+                TK +                            
Sbjct: 282  DEDD-IPISQRLKKSASSDDKSLSLKKTTKVVSSSFKKTTNKLKKAAKNSKYSKSTKMPP 340

Query: 1290 XXGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNK 1469
              GEGQKWTTL HNGVIFPP YKPHGVKMLY GKPVDLTPEQEEVATM+AVM +TDY  K
Sbjct: 341  SSGEGQKWTTLEHNGVIFPPLYKPHGVKMLYNGKPVDLTPEQEEVATMFAVMKDTDYATK 400

Query: 1470 PKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXX 1649
             +F ENFM DWR ILGKNH I+  E CDFTPIYEWH                        
Sbjct: 401  SRFIENFMNDWRTILGKNHVIKKFELCDFTPIYEWHQREKEKKKQMSAEEKKALKEEKLK 460

Query: 1650 XXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIP 1829
                YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI PSDITINIGK APIP
Sbjct: 461  QEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIHPSDITINIGKGAPIP 520

Query: 1830 ECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLK 2009
            ECPIPGE WKE+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKARLLK
Sbjct: 521  ECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK 580

Query: 2010 DYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV 2189
            DYI  IRA YT+DF SKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV
Sbjct: 581  DYIKDIRANYTRDFVSKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV 640

Query: 2190 EPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHL 2369
            E VPPN L+FDFLGKDSIRY N VEVE AV+KAI QF++GK+ G+DLFD LDTSKLNAHL
Sbjct: 641  ELVPPNKLQFDFLGKDSIRYFNTVEVELAVYKAIGQFKNGKEDGEDLFDSLDTSKLNAHL 700

Query: 2370 KELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSK 2549
            KELMPGLTAKVFRTYNASITLD+ML+RE++GGDVAEK+ VYQHANKEVAIICNHQR+VSK
Sbjct: 701  KELMPGLTAKVFRTYNASITLDEMLNRESKGGDVAEKIAVYQHANKEVAIICNHQRSVSK 760

Query: 2550 SHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQT 2729
            SHSAQM RL++KI+ELK  ++EL+TDLARAKKGKPPLK ADGKPKRNL P+ALE+KIAQ 
Sbjct: 761  SHSAQMHRLDDKIKELKDALDELKTDLARAKKGKPPLKGADGKPKRNLTPEALEKKIAQA 820

Query: 2730 TAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAW 2909
             AKIEKMERDK+ KEDLKTVALGTSKINYLDPRI+VAWCKRHEVPIEKIFNKSLLAKFAW
Sbjct: 821  NAKIEKMERDKQIKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAW 880

Query: 2910 AMDVDPGFRF 2939
            AMDVDP FRF
Sbjct: 881  AMDVDPDFRF 890


>ref|XP_023553297.1| DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo]
          Length = 887

 Score = 1060 bits (2742), Expect = 0.0
 Identities = 561/900 (62%), Positives = 647/900 (71%), Gaps = 7/900 (0%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPST-SVTDKS 617
            S NGQS+  +K  T  L KT P++  + S K+S +S     ++ P V S  +T  V+D  
Sbjct: 60   SANGQSSNAQKRMT-SLPKTSPMRPEIGSQKASKSS-----IQEPLVKSLGTTLKVSDSM 113

Query: 618  NSVKHQIKDEKLSTEATG-EPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 794
            +S   Q+ +  +  E     P +D+ DSEDDKPLS RL     K NSNH  K P   T+ 
Sbjct: 114  DSRPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQ 167

Query: 795  QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--K 968
            + +     EDSDDE+PLSSKFQ+KS +G    R     E+KPL SK  QNGS S ++  K
Sbjct: 168  KNKIKKSSEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQK 227

Query: 969  PSTNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 1142
            P+    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS R
Sbjct: 228  PAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQR 287

Query: 1143 MXXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQK-WTT 1319
            M               ++K                                G+GQK WTT
Sbjct: 288  MKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTT 347

Query: 1320 LVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMAD 1499
            LVHNGVIFPPPY+ HGVK+L+KGKPVDLTPEQEEVATM+AVM +TDYM KPKFKENF  D
Sbjct: 348  LVHNGVIFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWND 407

Query: 1500 WRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIV 1679
            WRK+LGKNH IQ+L+ CDFTPIY+W+                            YMWAI+
Sbjct: 408  WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAIL 467

Query: 1680 NGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWK 1859
            +GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WK
Sbjct: 468  DGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWK 527

Query: 1860 EIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAY 2039
            E+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +Y
Sbjct: 528  EVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSY 587

Query: 2040 TKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKF 2219
            TKDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F
Sbjct: 588  TKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEF 647

Query: 2220 DFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAK 2399
            +FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAK
Sbjct: 648  NFLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAK 707

Query: 2400 VFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLN 2579
            VFRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLN
Sbjct: 708  VFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLN 767

Query: 2580 EKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERD 2759
            EKI EL+ +V+EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD
Sbjct: 768  EKISELRDIVKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERD 827

Query: 2760 KETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
             +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 828  MKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_023007686.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007687.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007688.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007689.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
          Length = 887

 Score = 1054 bits (2726), Expect = 0.0
 Identities = 557/899 (61%), Positives = 646/899 (71%), Gaps = 6/899 (0%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
            S NGQS+  +K  T  L KT P++  + S K+S +S +   +KS     K S S+  +  
Sbjct: 60   STNGQSSNAQKRMT-SLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPK 118

Query: 621  S-VKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQ 797
              V   +K+E  S      P +++ DSEDDKPLS RL     K NSNH  K P   T+ +
Sbjct: 119  QLVNAAVKEENDSIH----PAQENSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQK 168

Query: 798  KRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--KP 971
             +     EDSDDE+PLSSKF++KS +G    +     E+KPL SK  QNGS S+++  KP
Sbjct: 169  NKIKKSSEDSDDEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKP 228

Query: 972  STNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRM 1145
            +    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS RM
Sbjct: 229  AVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM 288

Query: 1146 XXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQK-WTTL 1322
                           ++K                                G+GQK WTTL
Sbjct: 289  KMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTL 348

Query: 1323 VHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADW 1502
            VHNGVIFPPPY+ HGVK+L+KGKPVDLTPEQEEVATM+AVM +TDYM KPKFKENF  DW
Sbjct: 349  VHNGVIFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDW 408

Query: 1503 RKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIVN 1682
            RK+LGKNH IQ+L+ CDFTPIY+W+                            YMWAI++
Sbjct: 409  RKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILD 468

Query: 1683 GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKE 1862
            GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WKE
Sbjct: 469  GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKE 528

Query: 1863 IRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYT 2042
            +RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +YT
Sbjct: 529  VRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYT 588

Query: 2043 KDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFD 2222
            KDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F+
Sbjct: 589  KDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFN 648

Query: 2223 FLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKV 2402
            FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKV
Sbjct: 649  FLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKV 708

Query: 2403 FRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNE 2582
            FRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLNE
Sbjct: 709  FRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNE 768

Query: 2583 KIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDK 2762
            KI EL+ +++EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD 
Sbjct: 769  KISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDM 828

Query: 2763 ETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
            +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 829  KTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_022932254.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932255.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932256.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932257.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
          Length = 887

 Score = 1051 bits (2719), Expect = 0.0
 Identities = 556/900 (61%), Positives = 646/900 (71%), Gaps = 7/900 (0%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPST-SVTDKS 617
            S NGQS+  +K  T  L KT P++  + S K++ +S     ++ P V S  +T  V+D  
Sbjct: 60   SANGQSSNAQKRMT-SLPKTSPMRPEIGSQKAAKSS-----IQEPLVKSLGTTLKVSDSM 113

Query: 618  NSVKHQIKDEKLSTEATG-EPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 794
            +S   Q+ +  +  E     P +++ DSEDDKPLS RL     K NSNH  K P   T+ 
Sbjct: 114  DSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQ 167

Query: 795  QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--K 968
            + +     EDSDDE+PLSSKFQ+KS +G    +     E+KPL SK  QNGS S ++  K
Sbjct: 168  KNKIKKSSEDSDDEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQK 227

Query: 969  PSTNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 1142
            P+    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS R
Sbjct: 228  PAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQR 287

Query: 1143 MXXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQK-WTT 1319
            M               ++K                                G+GQK WTT
Sbjct: 288  MKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTT 347

Query: 1320 LVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMAD 1499
            LVHNGVIFPPPY+ HGVK+L+KGKPVDLT EQEEVATM+AVM +TDYM KPKFKENF  D
Sbjct: 348  LVHNGVIFPPPYQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWND 407

Query: 1500 WRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAIV 1679
            WRK+LGKNH IQ+L+ CDFTPIY+W+                            YMWAI+
Sbjct: 408  WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAIL 467

Query: 1680 NGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWK 1859
            +GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WK
Sbjct: 468  DGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWK 527

Query: 1860 EIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAY 2039
            E+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +Y
Sbjct: 528  EVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSY 587

Query: 2040 TKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKF 2219
            TKDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F
Sbjct: 588  TKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEF 647

Query: 2220 DFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAK 2399
            +FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAK
Sbjct: 648  NFLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAK 707

Query: 2400 VFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLN 2579
            VFRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLN
Sbjct: 708  VFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLN 767

Query: 2580 EKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERD 2759
            EKI EL+ +++EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD
Sbjct: 768  EKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERD 827

Query: 2760 KETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 2939
             +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 828  MKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_023748033.1| DNA topoisomerase 1 alpha-like [Lactuca sativa]
 gb|PLY62974.1| hypothetical protein LSAT_1X128740 [Lactuca sativa]
          Length = 788

 Score = 1036 bits (2680), Expect = 0.0
 Identities = 537/796 (67%), Positives = 599/796 (75%), Gaps = 10/796 (1%)
 Frame = +3

Query: 582  DSKPSTSVTDKSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNH 761
            D KP   +  + N+    +K     ++ + +P+ D  DSEDDKPLS R+   +SK ++NH
Sbjct: 7    DDKP---LIFRRNARPDTVKSSSQPSKPSPKPSNDEVDSEDDKPLSFRIST-VSKGDTNH 62

Query: 762  AKKSPNTSTLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQ 941
            AKK P TS     +     EDSDDE PLSSK+  KS  G S N        KP++ K  Q
Sbjct: 63   AKKGPGTSVSPLPKPSVKDEDSDDEKPLSSKYNSKSKVGSSNNH-------KPVVPKVHQ 115

Query: 942  NGSASTERKPSTN--LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDD 1115
            NGSAS +     N  L KRP D  ++     +KKPKLS++ TP  +K  ++KAE++ +DD
Sbjct: 116  NGSASKDNTQLKNIGLNKRPPDEERSAS---VKKPKLSDSVTPVNHKPVALKAEIKSDDD 172

Query: 1116 EDHVPISHRMXXXXXXXXXXXXXXNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1295
            +D   +                  ++ + +                              
Sbjct: 173  DDDDDVPISQRIKKQAPSSSNKSSSVKQKVMKKSSSSLNQTNKKSKKMMKQKQSKYSKPS 232

Query: 1296 ------GEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETD 1457
                  GEGQKWT+LVH+GVIFPP YKPHGVKMLYKGKPVDLTPEQEEVATM++VML+TD
Sbjct: 233  KIPPGSGEGQKWTSLVHSGVIFPPEYKPHGVKMLYKGKPVDLTPEQEEVATMFSVMLDTD 292

Query: 1458 YMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXX 1637
            YM KP+FKENF  DWRKILGKNHTIQ+LE+CDF PIYEWH                    
Sbjct: 293  YMAKPQFKENFWGDWRKILGKNHTIQNLENCDFKPIYEWHQAEKEKKKLMTTEEKKAIKE 352

Query: 1638 XXXXXXXXYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD 1817
                    YMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD
Sbjct: 353  EKMKIEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD 412

Query: 1818 APIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKA 1997
            APIPECPIPGE WKEIRHDNTVTWLAYWNDPIN KEFKYVFLAASS+LKGQSDKEKYEKA
Sbjct: 413  APIPECPIPGERWKEIRHDNTVTWLAYWNDPINSKEFKYVFLAASSSLKGQSDKEKYEKA 472

Query: 1998 RLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 2177
            R LK YI GIR AYTKDF + D  KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLK
Sbjct: 473  RKLKGYIEGIRKAYTKDFGNSDVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 532

Query: 2178 VENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKL 2357
            VENVEP PPN LKFDFLGKDSIRYQNEVEVE  VFKAIQQFR+GK G DDLFD+LDTSKL
Sbjct: 533  VENVEPEPPNILKFDFLGKDSIRYQNEVEVELPVFKAIQQFRTGKAGNDDLFDKLDTSKL 592

Query: 2358 NAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQR 2537
            NAHLKELMPGLTAKVFRTYNASITLDDML+RET+GGDVA+ +VVYQHANKEVAIICNHQR
Sbjct: 593  NAHLKELMPGLTAKVFRTYNASITLDDMLTRETKGGDVAQNLVVYQHANKEVAIICNHQR 652

Query: 2538 TVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPP--LKSADGKPKRNLNPDALE 2711
            TVSKSHSAQM RLNEKI+ELKGV++EL++DLARAKKGKPP      DGKPKRNLNP+ALE
Sbjct: 653  TVSKSHSAQMTRLNEKIDELKGVIKELESDLARAKKGKPPSNKNGPDGKPKRNLNPEALE 712

Query: 2712 RKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSL 2891
            +KIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSL
Sbjct: 713  KKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKLFNKSL 772

Query: 2892 LAKFAWAMDVDPGFRF 2939
            LAKFAW+MDVDP FRF
Sbjct: 773  LAKFAWSMDVDPSFRF 788


>gb|PON81509.1| DNA topoisomerase I [Trema orientalis]
          Length = 897

 Score = 1029 bits (2660), Expect = 0.0
 Identities = 548/901 (60%), Positives = 637/901 (70%), Gaps = 8/901 (0%)
 Frame = +3

Query: 261  MAVEACDKQKLXXXXXXXXXPLVFKRXXXXXXXXXXXXEAKKSLSQKHDRQLGRPAPDVR 440
            MAV+A  K  L         P+VFKR            E KK LSQK D Q GR   D  
Sbjct: 1    MAVQAPAKPNLVDDYDEDE-PIVFKRTNPISKQNQLNSEVKKPLSQKPDGQSGRQISDRV 59

Query: 441  SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 620
            S N  ++  +K K V  ++   VK  + SPK+S +S K   LKSP  +SK STS+  +S 
Sbjct: 60   SLNDLNSSSQKGKVVQSNRALVVKSSVASPKASASSAKTSPLKSPITNSKTSTSLDGQSK 119

Query: 621  SVKHQ-----IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTS 785
                +     +K+EK S +     +  S+DSEDD PLSARL    S+  S    K    S
Sbjct: 120  FSLEKNKSSIVKEEKSSIKQPIRSDSHSEDSEDDIPLSARLKGSTSQ-GSKGLVKPTTAS 178

Query: 786  TLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE- 962
             L    +  + ED  D  PLSS+FQ+K N G S ++ ++  ++KPLLS   QNGS  T+ 
Sbjct: 179  KLPPVTKSVVKEDDSDNEPLSSRFQMKPNVGTSGSKPNDFNDKKPLLSINKQNGSTVTDV 238

Query: 963  RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 1142
            +KP     KRP D  K+ D+S +K+PKLS++    KNK+ S+KAE + +DD DHVPIS R
Sbjct: 239  KKPLNTNTKRPLDREKS-DQSSVKRPKLSDSPALLKNKQVSVKAESKVDDD-DHVPISQR 296

Query: 1143 MXXXXXXXXXXXXXXN-ITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEGQK-WT 1316
            +                +TKP+                              G+GQK WT
Sbjct: 297  IKKSPASNNKSSTTKQKVTKPVSSSFKKTTKKFKKGAKNSKYSKSTKLSPSSGDGQKKWT 356

Query: 1317 TLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMA 1496
            TL+HNGVIFPPPY+PHGVK+ Y GKPVDLTP QEEVATM+AVM +TDY  K  F+ENF  
Sbjct: 357  TLIHNGVIFPPPYQPHGVKLFYNGKPVDLTPVQEEVATMFAVMKDTDYAEKKTFRENFWN 416

Query: 1497 DWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXXYMWAI 1676
            DWRK+LGKNH IQ L+ CDFTPIY+W+                            YMWAI
Sbjct: 417  DWRKLLGKNHVIQKLDLCDFTPIYDWYQNEKEKKKQMSADEKKALKEEKLKQEEKYMWAI 476

Query: 1677 VNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESW 1856
            V+GVKEKVGNFRVEPPGLFRGRGEHPK GKLK+RIRPSD+TINIGKDAP+PECPIPGE W
Sbjct: 477  VDGVKEKVGNFRVEPPGLFRGRGEHPKSGKLKRRIRPSDVTINIGKDAPVPECPIPGERW 536

Query: 1857 KEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAA 2036
            KE+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IRAA
Sbjct: 537  KEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIHNIRAA 596

Query: 2037 YTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLK 2216
            YTKDFTSKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ + PN L+
Sbjct: 597  YTKDFTSKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAIAPNKLE 656

Query: 2217 FDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTA 2396
            F+FLGKDSIRY+N VEVE  V+KAI QF+SGKKG DDLFD LDTSKLNAHLKELMPGLTA
Sbjct: 657  FNFLGKDSIRYENTVEVELPVYKAIIQFQSGKKGNDDLFDLLDTSKLNAHLKELMPGLTA 716

Query: 2397 KVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRL 2576
            KVFRTYNASITLDDML++ET+ GD+ EK  VY  ANK+VAIICNHQRT+SKSH AQM +L
Sbjct: 717  KVFRTYNASITLDDMLNQETKDGDLEEKKTVYNTANKQVAIICNHQRTISKSHEAQMSKL 776

Query: 2577 NEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMER 2756
             EKI EL+ VV+EL+ DL RA+KGKPPLK ADGK KRNL+P+AL +KI QT  KIEK++R
Sbjct: 777  MEKIRELEDVVKELEVDLERARKGKPPLKDADGKTKRNLSPEALGKKINQTNTKIEKIKR 836

Query: 2757 DKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFR 2936
            D + KED KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAW+MDVDP FR
Sbjct: 837  DMQNKEDTKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVDPDFR 896

Query: 2937 F 2939
            F
Sbjct: 897  F 897