BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00002010
         (2689 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011094716.1| E3 ubiquitin-protein ligase UPL4 isoform X1 ...  1273   0.0  
gb|PIN18482.1| E3 ubiquitin protein ligase [Handroanthus impetig...  1261   0.0  
ref|XP_020553307.1| E3 ubiquitin-protein ligase UPL4 isoform X2 ...  1207   0.0  
ref|XP_012831928.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...  1108   0.0  
ref|XP_012831927.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...  1100   0.0  
gb|KZV58123.1| hypothetical protein F511_37571 [Dorcoceras hygro...  1077   0.0  
ref|XP_020553308.1| E3 ubiquitin-protein ligase UPL4 isoform X3 ...  1048   0.0  
ref|XP_019265055.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   949   0.0  
ref|XP_009622740.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   947   0.0  
ref|XP_019265058.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   944   0.0  
ref|XP_016503890.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-...   944   0.0  
ref|XP_009794383.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   944   0.0  
ref|XP_009622742.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   942   0.0  
ref|XP_019077234.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   942   0.0  
ref|XP_019077233.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   942   0.0  
ref|XP_010653449.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   942   0.0  
gb|PHU06662.1| E3 ubiquitin-protein ligase UPL4 [Capsicum chinense]   941   0.0  
ref|XP_019191349.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   939   0.0  
ref|XP_016503911.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-...   939   0.0  
ref|XP_009794443.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 ...   939   0.0  

>ref|XP_011094716.1| E3 ubiquitin-protein ligase UPL4 isoform X1 [Sesamum indicum]
 ref|XP_011094717.1| E3 ubiquitin-protein ligase UPL4 isoform X1 [Sesamum indicum]
 ref|XP_020553305.1| E3 ubiquitin-protein ligase UPL4 isoform X1 [Sesamum indicum]
 ref|XP_020553306.1| E3 ubiquitin-protein ligase UPL4 isoform X1 [Sesamum indicum]
          Length = 1551

 Score = 1273 bits (3293), Expect = 0.0
 Identities = 649/878 (73%), Positives = 713/878 (81%)
 Frame = -2

Query: 2688 HLVGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPI 2509
            H+VG+EDNNA N+L IMEKRFE+FGRLLLSC +  REEFPL+ LIRRLQSALSSVENFP+
Sbjct: 682  HIVGREDNNAVNNLCIMEKRFELFGRLLLSCDNTAREEFPLLILIRRLQSALSSVENFPV 741

Query: 2508 ISSHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGR 2329
            ISSHT + RNSYATVPYGRCTSYPCLKVQFVRE GEV LRD A+DVVNVDPFV L+EI  
Sbjct: 742  ISSHTARRRNSYATVPYGRCTSYPCLKVQFVREKGEVSLRDCADDVVNVDPFVALEEIEG 801

Query: 2328 YLWPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQE 2149
            YL PRV+N KTK L   SK SKE+D                  DV  S EMLVD  K Q+
Sbjct: 802  YLLPRVTNGKTKILRSESKGSKEKDSSSSHSPSDSSICQAKSIDVIKSTEMLVDFHKLQD 861

Query: 2148 AKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNED 1969
             + NLL SSPA  SS +Q  +DSA VAD QTD +E +EH+PL+EDGG N DHPG SD E+
Sbjct: 862  KESNLLLSSPADTSSSSQRIMDSADVADVQTDPLEPKEHDPLQEDGGTNFDHPGCSDCEE 921

Query: 1968 TSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNH 1789
            TS KLLFYLE QQLNC+LTLYQSIL  Q   + D I S S+W RI+K+TY+R + T+  H
Sbjct: 922  TSPKLLFYLESQQLNCKLTLYQSILNLQTETDHDNISSASLWNRIYKLTYRRPVTTRVRH 981

Query: 1788 AKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRLRF 1609
             K  HDE   SL  KRA FFQY P F P+F SEVD EK  P YD+LSLL+SLEGINRLRF
Sbjct: 982  PKPSHDEAQCSLSLKRALFFQYTPYFCPMFASEVDLEKLGPTYDILSLLKSLEGINRLRF 1041

Query: 1608 HLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPA 1429
            HLMS ERTY F EG T DLDKLNV V+EVPPNEFVNKKLT+KL+QQMRDP AVSVGAMPA
Sbjct: 1042 HLMSRERTYAFAEGRTDDLDKLNVVVSEVPPNEFVNKKLTEKLEQQMRDPMAVSVGAMPA 1101

Query: 1428 WCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKV 1249
            WCTQLM WCPFLFGFEARCKYFHLAALG+SP Q              RQQ+     R+K+
Sbjct: 1102 WCTQLMAWCPFLFGFEARCKYFHLAALGRSPVQTHSVSHGNAGGSGGRQQS-----RRKI 1156

Query: 1248 LVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1069
            LVHRNKILESAAQMMELH   KVL EVEY+EEVGTGLGPTLEFYTLVCHEFQRSGLGMWR
Sbjct: 1157 LVHRNKILESAAQMMELHTHQKVLFEVEYDEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1216

Query: 1068 DDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKS 889
            DD + L C+  LE EN+G +VS  GLFPRPW            S+VI+KF+LLG IVAK+
Sbjct: 1217 DDTVPLQCTAVLETENTGFLVSPFGLFPRPWSPSLSASSSSVYSDVIEKFSLLGYIVAKA 1276

Query: 888  LQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEK 709
            LQDGRVLDLPFSKA YKLILGK+L+LYDIQSFDPA G ALLEFQA+VE+++YLRS+C E+
Sbjct: 1277 LQDGRVLDLPFSKALYKLILGKELSLYDIQSFDPASGRALLEFQAVVERKEYLRSVCKEE 1336

Query: 708  SHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTK 529
            S DLDV LR+TKIEDLCLDFTLPGYPDY LV     +DSRMVNLYNLDEY+ L+VDATTK
Sbjct: 1337 SADLDVCLRNTKIEDLCLDFTLPGYPDYVLV---PETDSRMVNLYNLDEYITLIVDATTK 1393

Query: 528  SGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVS 349
            SGIARQVEAFKSGFDQVFPI+HLKVFTEEELERLLCGEHVLWNSE+LLDHIKFDHGYT+S
Sbjct: 1394 SGIARQVEAFKSGFDQVFPIKHLKVFTEEELERLLCGEHVLWNSEDLLDHIKFDHGYTIS 1453

Query: 348  SPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLP 169
            SPPIVNLLEIMQEFDL QQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCS  IDADLP
Sbjct: 1454 SPPIVNLLEIMQEFDLKQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSKGIDADLP 1513

Query: 168  SVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            SVMTCANYLKLPPYSSKEVMKEKLLYA+ EGQGSFHLS
Sbjct: 1514 SVMTCANYLKLPPYSSKEVMKEKLLYAVTEGQGSFHLS 1551


>gb|PIN18482.1| E3 ubiquitin protein ligase [Handroanthus impetiginosus]
          Length = 1545

 Score = 1261 bits (3262), Expect = 0.0
 Identities = 650/878 (74%), Positives = 713/878 (81%)
 Frame = -2

Query: 2688 HLVGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPI 2509
            HLV +ED+NAAN L +MEKRFE+FGRLLLSC D  +EEFPL+ LI RLQSALSSVENFP+
Sbjct: 682  HLVEQEDDNAANCLHVMEKRFEVFGRLLLSCADSGQEEFPLLMLIHRLQSALSSVENFPV 741

Query: 2508 ISSHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGR 2329
            ISS+T + RNSYA VPYG  TSYPCLKVQFVRENGE CLRDYAED VNVDPFVPL+EI  
Sbjct: 742  ISSNTIRRRNSYAIVPYGHYTSYPCLKVQFVRENGETCLRDYAEDFVNVDPFVPLEEIEE 801

Query: 2328 YLWPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQE 2149
            YL PRVSN KT+NLTL SKDS+E+D                 +DV  S EMLVD  KQQ 
Sbjct: 802  YLRPRVSNAKTENLTLRSKDSREKDSLYSYSPSDSSTSQGRSADVMVSTEMLVDGHKQQR 861

Query: 2148 AKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNED 1969
             KPN+L SSPA  SSFAQ  +D+A V D QTD VEQE+HNP+ EDG AN D  G SD E+
Sbjct: 862  VKPNMLLSSPAETSSFAQKVMDAAGVTDVQTDAVEQEKHNPIHEDGDANTDMLGCSDTEE 921

Query: 1968 TSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNH 1789
            T  KLL YLEG+Q+N ++TLYQSIL+Q   + RD IP+ S+WT +HKITYKRH  TKP++
Sbjct: 922  TKEKLLLYLEGRQMNSKMTLYQSILEQLAEIHRDDIPTASLWTCVHKITYKRHSTTKPSN 981

Query: 1788 AKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRLRF 1609
            A+  H E+L SLLSK+A   QY P FS + V  VDFEK  P  D+L+LL+SLEGINRLRF
Sbjct: 982  AQHNH-ESLCSLLSKKAPLVQYTPCFSHVLVPGVDFEKFGPTNDILTLLKSLEGINRLRF 1040

Query: 1608 HLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPA 1429
            HLMS ERT  F EG T DLDKLNV+V EVP  EFVNKKLT+KL+QQMRDP AVSVGAMPA
Sbjct: 1041 HLMSRERTNAFAEGRTDDLDKLNVSVYEVPQVEFVNKKLTEKLEQQMRDPMAVSVGAMPA 1100

Query: 1428 WCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKV 1249
            WCTQLMDWCPFLFGFEARCKYFHLAALG+ PSQ              RQQNH  +PRKK+
Sbjct: 1101 WCTQLMDWCPFLFGFEARCKYFHLAALGRVPSQTQSTSHSNPGGSSGRQQNHENIPRKKI 1160

Query: 1248 LVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1069
            LVHRNKILESAAQMMELH R KVL EVEYNEEVGTGLGPTLEFYTLVCHEFQRS LGMWR
Sbjct: 1161 LVHRNKILESAAQMMELHARQKVLLEVEYNEEVGTGLGPTLEFYTLVCHEFQRSSLGMWR 1220

Query: 1068 DDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKS 889
                      DLEAENSGL+VS   LFPRPW            SEV KKFALLGQIVAK+
Sbjct: 1221 ----------DLEAENSGLLVSPFALFPRPWSPLVSTSSSTVYSEVTKKFALLGQIVAKA 1270

Query: 888  LQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEK 709
            LQDGRVLDLPFSKAFYKLILGK+LTLYDIQSFDPA G ALLEFQA+VE++  LRSL  EK
Sbjct: 1271 LQDGRVLDLPFSKAFYKLILGKELTLYDIQSFDPALGRALLEFQAVVERKKCLRSLYDEK 1330

Query: 708  SHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTK 529
            S +L+V LRDTKIEDLCLDFTLPGYPDYALVS   +SDS  VNL+NL+EYV L+VDAT K
Sbjct: 1331 SQELNVCLRDTKIEDLCLDFTLPGYPDYALVS---TSDSITVNLHNLEEYVTLVVDATIK 1387

Query: 528  SGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVS 349
            SGIARQVEAFKSGFDQVFPIRHLKVFTEEE+ERLLCGEHVLWNS+ELLDHIKFDHGYT+S
Sbjct: 1388 SGIARQVEAFKSGFDQVFPIRHLKVFTEEEMERLLCGEHVLWNSDELLDHIKFDHGYTIS 1447

Query: 348  SPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLP 169
            SPPI+NLLEIMQEF+L+QQRAFLQFVTG+PRLPTGGLASLNPKLTIVRKHCS  IDADLP
Sbjct: 1448 SPPIINLLEIMQEFNLEQQRAFLQFVTGSPRLPTGGLASLNPKLTIVRKHCSKGIDADLP 1507

Query: 168  SVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            SVMTCANYLKLPPYSSKEVMKEKLLYAI EGQGSFHLS
Sbjct: 1508 SVMTCANYLKLPPYSSKEVMKEKLLYAITEGQGSFHLS 1545


>ref|XP_020553307.1| E3 ubiquitin-protein ligase UPL4 isoform X2 [Sesamum indicum]
          Length = 1517

 Score = 1207 bits (3122), Expect = 0.0
 Identities = 617/844 (73%), Positives = 680/844 (80%)
 Frame = -2

Query: 2688 HLVGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPI 2509
            H+VG+EDNNA N+L IMEKRFE+FGRLLLSC +  REEFPL+ LIRRLQSALSSVENFP+
Sbjct: 682  HIVGREDNNAVNNLCIMEKRFELFGRLLLSCDNTAREEFPLLILIRRLQSALSSVENFPV 741

Query: 2508 ISSHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGR 2329
            ISSHT + RNSYATVPYGRCTSYPCLKVQFVRE GEV LRD A+DVVNVDPFV L+EI  
Sbjct: 742  ISSHTARRRNSYATVPYGRCTSYPCLKVQFVREKGEVSLRDCADDVVNVDPFVALEEIEG 801

Query: 2328 YLWPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQE 2149
            YL PRV+N KTK L   SK SKE+D                  DV  S EMLVD  K Q+
Sbjct: 802  YLLPRVTNGKTKILRSESKGSKEKDSSSSHSPSDSSICQAKSIDVIKSTEMLVDFHKLQD 861

Query: 2148 AKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNED 1969
             + NLL SSPA  SS +Q  +DSA VAD QTD +E +EH+PL+EDGG N DHPG SD E+
Sbjct: 862  KESNLLLSSPADTSSSSQRIMDSADVADVQTDPLEPKEHDPLQEDGGTNFDHPGCSDCEE 921

Query: 1968 TSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNH 1789
            TS KLLFYLE QQLNC+LTLYQSIL  Q   + D I S S+W RI+K+TY+R + T+  H
Sbjct: 922  TSPKLLFYLESQQLNCKLTLYQSILNLQTETDHDNISSASLWNRIYKLTYRRPVTTRVRH 981

Query: 1788 AKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRLRF 1609
             K  HDE   SL  KRA FFQY P F P+F SEVD EK  P YD+LSLL+SLEGINRLRF
Sbjct: 982  PKPSHDEAQCSLSLKRALFFQYTPYFCPMFASEVDLEKLGPTYDILSLLKSLEGINRLRF 1041

Query: 1608 HLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPA 1429
            HLMS ERTY F EG T DLDKLNV V+EVPPNEFVNKKLT+KL+QQMRDP AVSVGAMPA
Sbjct: 1042 HLMSRERTYAFAEGRTDDLDKLNVVVSEVPPNEFVNKKLTEKLEQQMRDPMAVSVGAMPA 1101

Query: 1428 WCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKV 1249
            WCTQLM WCPFLFGFEARCKYFHLAALG+SP Q              RQQ+     R+K+
Sbjct: 1102 WCTQLMAWCPFLFGFEARCKYFHLAALGRSPVQTHSVSHGNAGGSGGRQQS-----RRKI 1156

Query: 1248 LVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1069
            LVHRNKILESAAQMMELH   KVL EVEY+EEVGTGLGPTLEFYTLVCHEFQRSGLGMWR
Sbjct: 1157 LVHRNKILESAAQMMELHTHQKVLFEVEYDEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1216

Query: 1068 DDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKS 889
            DD + L C+  LE EN+G +VS  GLFPRPW            S+VI+KF+LLG IVAK+
Sbjct: 1217 DDTVPLQCTAVLETENTGFLVSPFGLFPRPWSPSLSASSSSVYSDVIEKFSLLGYIVAKA 1276

Query: 888  LQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEK 709
            LQDGRVLDLPFSKA YKLILGK+L+LYDIQSFDPA G ALLEFQA+VE+++YLRS+C E+
Sbjct: 1277 LQDGRVLDLPFSKALYKLILGKELSLYDIQSFDPASGRALLEFQAVVERKEYLRSVCKEE 1336

Query: 708  SHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTK 529
            S DLDV LR+TKIEDLCLDFTLPGYPDY LV     +DSRMVNLYNLDEY+ L+VDATTK
Sbjct: 1337 SADLDVCLRNTKIEDLCLDFTLPGYPDYVLV---PETDSRMVNLYNLDEYITLIVDATTK 1393

Query: 528  SGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVS 349
            SGIARQVEAFKSGFDQVFPI+HLKVFTEEELERLLCGEHVLWNSE+LLDHIKFDHGYT+S
Sbjct: 1394 SGIARQVEAFKSGFDQVFPIKHLKVFTEEELERLLCGEHVLWNSEDLLDHIKFDHGYTIS 1453

Query: 348  SPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLP 169
            SPPIVNLLEIMQEFDL QQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCS  IDADLP
Sbjct: 1454 SPPIVNLLEIMQEFDLKQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSKGIDADLP 1513

Query: 168  SVMT 157
            SVMT
Sbjct: 1514 SVMT 1517


>ref|XP_012831928.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X2 [Erythranthe
            guttata]
 gb|EYU41635.1| hypothetical protein MIMGU_mgv1a000163mg [Erythranthe guttata]
          Length = 1517

 Score = 1108 bits (2866), Expect = 0.0
 Identities = 577/877 (65%), Positives = 668/877 (76%), Gaps = 1/877 (0%)
 Frame = -2

Query: 2682 VGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIIS 2503
            +G+E  +AA+H   M KRFE+FG+LL+SC DP  E+  ++ LI+RLQ+ALSSVENFP+I 
Sbjct: 684  IGQEVTHAADHFCTMGKRFEVFGQLLMSCTDPASEKSLILALIQRLQNALSSVENFPVIP 743

Query: 2502 SHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYL 2323
            SH Y++RN YATVP G CT YPCLKVQFVRE  E CLRDYA+D+VNVDPFVPL+EI  YL
Sbjct: 744  SHAYRTRNYYATVPSGHCTPYPCLKVQFVREKEENCLRDYADDIVNVDPFVPLEEIEGYL 803

Query: 2322 WPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
             PRV NDKT N TL S+DSK +D                 +D  A    +VDV + QE K
Sbjct: 804  LPRVRNDKTVNSTLRSEDSKLKDSTCSPSPSDSSTPRAKSADDIAP---MVDVDELQEVK 860

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
            PN+L SSP   SS AQ  +D+        D V+QE HNPL+++   +      +D+EDT 
Sbjct: 861  PNVL-SSPTNISSSAQKVMDAV------EDSVDQEGHNPLQQEASTS------TDSEDTP 907

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAK 1783
              L  YLEGQ+LNC+LTLYQSILKQQ G E D +   ++W+R++KITY RH  T+  H K
Sbjct: 908  ASLQLYLEGQELNCKLTLYQSILKQQTGTEHDSMSIATLWSRVYKITYGRHATTERIHCK 967

Query: 1782 RIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRLRFHL 1603
            R HDE   SLL ++ +F QY P F  +F+S  + E+  P+YD++SLL+SLEGINRLRFHL
Sbjct: 968  RSHDEFTLSLLCEK-TFSQYTPYFCRMFLSNAEAEELGPSYDVISLLKSLEGINRLRFHL 1026

Query: 1602 MSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPAWC 1423
            MS E T  F EG T DL+KLN A+ EV  NEFVNKKLT+KL+QQMR+P A SVGAMPAWC
Sbjct: 1027 MSRETTRVFAEGRTDDLNKLNSAICEVHQNEFVNKKLTEKLEQQMRNPIAASVGAMPAWC 1086

Query: 1422 TQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXA-RQQNHGYLPRKKVL 1246
            T LM+WCPFLFGFEARCKYFH+AALG+ P+              + R QN    PRKK+L
Sbjct: 1087 TLLMNWCPFLFGFEARCKYFHIAALGRLPNHTQSTSHGNNGGGSSGRHQN----PRKKIL 1142

Query: 1245 VHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRD 1066
            VHRNKILESAA MMELH R KV+ EVEY+EEVGTGLGPTLEFYTLVCHEFQRSGLGMWRD
Sbjct: 1143 VHRNKILESAAHMMELHSRQKVVLEVEYSEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRD 1202

Query: 1065 DCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKSL 886
            DC+                VS  GLFPRPW             EVIKKF LLG IVAK++
Sbjct: 1203 DCL----------------VSLFGLFPRPWSPSSSSTVHS---EVIKKFTLLGHIVAKAI 1243

Query: 885  QDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEKS 706
            QDGR+LDLPF+KAFYKLILGK+L+LYDIQSFDPA G ALLEFQA+VE++ YL+S C + S
Sbjct: 1244 QDGRLLDLPFAKAFYKLILGKELSLYDIQSFDPALGRALLEFQAVVERKQYLKSHCEDSS 1303

Query: 705  HDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTKS 526
             D+DV LR+TKIED+CLDF+LPGYPDY L SG    DS+MVNL+NL+EYV LMVDATTKS
Sbjct: 1304 RDVDVLLRNTKIEDMCLDFSLPGYPDYVLASGL---DSKMVNLHNLEEYVALMVDATTKS 1360

Query: 525  GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVSS 346
            GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHV+WNS+ELLDHIKFDHGYT+SS
Sbjct: 1361 GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVIWNSDELLDHIKFDHGYTISS 1420

Query: 345  PPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLPS 166
            PPI NLLEIM+EFDL QQRAFLQFVTGAPRLPTGGLASL+P LTIVRKHCS  ID DLPS
Sbjct: 1421 PPIANLLEIMKEFDLKQQRAFLQFVTGAPRLPTGGLASLSPNLTIVRKHCSKGIDDDLPS 1480

Query: 165  VMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            VMTCANYLKLPPYSSKEVM+EKLLYAI EGQGSFHLS
Sbjct: 1481 VMTCANYLKLPPYSSKEVMREKLLYAITEGQGSFHLS 1517


>ref|XP_012831927.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Erythranthe
            guttata]
          Length = 1527

 Score = 1100 bits (2845), Expect = 0.0
 Identities = 577/887 (65%), Positives = 668/887 (75%), Gaps = 11/887 (1%)
 Frame = -2

Query: 2682 VGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIIS 2503
            +G+E  +AA+H   M KRFE+FG+LL+SC DP  E+  ++ LI+RLQ+ALSSVENFP+I 
Sbjct: 684  IGQEVTHAADHFCTMGKRFEVFGQLLMSCTDPASEKSLILALIQRLQNALSSVENFPVIP 743

Query: 2502 SHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYL 2323
            SH Y++RN YATVP G CT YPCLKVQFVRE  E CLRDYA+D+VNVDPFVPL+EI  YL
Sbjct: 744  SHAYRTRNYYATVPSGHCTPYPCLKVQFVREKEENCLRDYADDIVNVDPFVPLEEIEGYL 803

Query: 2322 WPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQ--- 2152
             PRV NDKT N TL S+DSK +D                 +D  A    +VDV + Q   
Sbjct: 804  LPRVRNDKTVNSTLRSEDSKLKDSTCSPSPSDSSTPRAKSADDIAP---MVDVDELQVYT 860

Query: 2151 -------EAKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDH 1993
                   E KPN+L SSP   SS AQ  +D+        D V+QE HNPL+++   +   
Sbjct: 861  RLRQFKLEVKPNVL-SSPTNISSSAQKVMDAV------EDSVDQEGHNPLQQEASTS--- 910

Query: 1992 PGFSDNEDTSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKR 1813
               +D+EDT   L  YLEGQ+LNC+LTLYQSILKQQ G E D +   ++W+R++KITY R
Sbjct: 911  ---TDSEDTPASLQLYLEGQELNCKLTLYQSILKQQTGTEHDSMSIATLWSRVYKITYGR 967

Query: 1812 HMATKPNHAKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSL 1633
            H  T+  H KR HDE   SLL ++ +F QY P F  +F+S  + E+  P+YD++SLL+SL
Sbjct: 968  HATTERIHCKRSHDEFTLSLLCEK-TFSQYTPYFCRMFLSNAEAEELGPSYDVISLLKSL 1026

Query: 1632 EGINRLRFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTA 1453
            EGINRLRFHLMS E T  F EG T DL+KLN A+ EV  NEFVNKKLT+KL+QQMR+P A
Sbjct: 1027 EGINRLRFHLMSRETTRVFAEGRTDDLNKLNSAICEVHQNEFVNKKLTEKLEQQMRNPIA 1086

Query: 1452 VSVGAMPAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXA-RQQN 1276
             SVGAMPAWCT LM+WCPFLFGFEARCKYFH+AALG+ P+              + R QN
Sbjct: 1087 ASVGAMPAWCTLLMNWCPFLFGFEARCKYFHIAALGRLPNHTQSTSHGNNGGGSSGRHQN 1146

Query: 1275 HGYLPRKKVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEF 1096
                PRKK+LVHRNKILESAA MMELH R KV+ EVEY+EEVGTGLGPTLEFYTLVCHEF
Sbjct: 1147 ----PRKKILVHRNKILESAAHMMELHSRQKVVLEVEYSEEVGTGLGPTLEFYTLVCHEF 1202

Query: 1095 QRSGLGMWRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFA 916
            QRSGLGMWRDDC+                VS  GLFPRPW             EVIKKF 
Sbjct: 1203 QRSGLGMWRDDCL----------------VSLFGLFPRPWSPSSSSTVHS---EVIKKFT 1243

Query: 915  LLGQIVAKSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRD 736
            LLG IVAK++QDGR+LDLPF+KAFYKLILGK+L+LYDIQSFDPA G ALLEFQA+VE++ 
Sbjct: 1244 LLGHIVAKAIQDGRLLDLPFAKAFYKLILGKELSLYDIQSFDPALGRALLEFQAVVERKQ 1303

Query: 735  YLRSLCGEKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYV 556
            YL+S C + S D+DV LR+TKIED+CLDF+LPGYPDY L SG    DS+MVNL+NL+EYV
Sbjct: 1304 YLKSHCEDSSRDVDVLLRNTKIEDMCLDFSLPGYPDYVLASGL---DSKMVNLHNLEEYV 1360

Query: 555  MLMVDATTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHI 376
             LMVDATTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHV+WNS+ELLDHI
Sbjct: 1361 ALMVDATTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVIWNSDELLDHI 1420

Query: 375  KFDHGYTVSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHC 196
            KFDHGYT+SSPPI NLLEIM+EFDL QQRAFLQFVTGAPRLPTGGLASL+P LTIVRKHC
Sbjct: 1421 KFDHGYTISSPPIANLLEIMKEFDLKQQRAFLQFVTGAPRLPTGGLASLSPNLTIVRKHC 1480

Query: 195  SNAIDADLPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            S  ID DLPSVMTCANYLKLPPYSSKEVM+EKLLYAI EGQGSFHLS
Sbjct: 1481 SKGIDDDLPSVMTCANYLKLPPYSSKEVMREKLLYAITEGQGSFHLS 1527


>gb|KZV58123.1| hypothetical protein F511_37571 [Dorcoceras hygrometricum]
          Length = 1527

 Score = 1077 bits (2786), Expect = 0.0
 Identities = 553/878 (62%), Positives = 651/878 (74%)
 Frame = -2

Query: 2688 HLVGKEDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPI 2509
            H VG+E N+  N     EK+ E FGRLL SC DPT E+F L+ L+RRLQSALSSVENFP+
Sbjct: 679  HHVGQETNDTTNQYNTTEKKIEEFGRLLFSCTDPTLEKFSLLILVRRLQSALSSVENFPV 738

Query: 2508 ISSHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGR 2329
            I +   K RNSYATVPYGRCTSYPCLKVQF RE GE+ LRDY+ED+VNVDPF+PLD I  
Sbjct: 739  ILNQACKLRNSYATVPYGRCTSYPCLKVQFEREKGEISLRDYSEDIVNVDPFLPLDAIEG 798

Query: 2328 YLWPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQE 2149
            YLWPRVS     N TL SKD KE +                 +D   SKEM+VDV KQQ+
Sbjct: 799  YLWPRVSKIDPNNSTLWSKDLKENNTSCSQSPAYSSDSVGKIADAIVSKEMVVDVHKQQD 858

Query: 2148 AKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNED 1969
                +L SSP   +S  +   DSA +    T   EQEE N L++D    ++ PG S++ED
Sbjct: 859  GTQEILLSSPRDTASSKREIPDSAKLTVVHTGTSEQEEENTLQKDDAMKIEQPGHSNSED 918

Query: 1968 TSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNH 1789
            +S KLLFYLEG+QL+C+LT+YQSIL+QQ  +E DIIPS+S+WTRI+KITYKR        
Sbjct: 919  SSQKLLFYLEGKQLDCKLTMYQSILEQQTELEHDIIPSSSLWTRIYKITYKR-------- 970

Query: 1788 AKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRLRF 1609
                             SF Q + L S     EVD EK   A+D+L LLRSLE INR++ 
Sbjct: 971  ----------------TSFCQSSSLNSQTAFPEVDVEKLGSAHDVLFLLRSLERINRMKL 1014

Query: 1608 HLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPA 1429
            HLMS ERT+   EG T D+DK +V  ++V  +EFVN KL++KL+QQMRDP AVSVG MPA
Sbjct: 1015 HLMSRERTHACAEGRTDDMDKFDVTFSDVSQSEFVNSKLSEKLEQQMRDPLAVSVGGMPA 1074

Query: 1428 WCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKV 1249
            WC+ LM W PFLF FEA+CKYFHLAALG+  +Q              RQQNHG   RKK 
Sbjct: 1075 WCSHLMAWYPFLFAFEAKCKYFHLAALGRLSAQILSSSQTYVGSSSGRQQNHGNFQRKKF 1134

Query: 1248 LVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWR 1069
            LV+RN +LESAA MMELH   KVL EVEY EEVGTGLGPTLEFYTLVCHEFQR+GLG+WR
Sbjct: 1135 LVNRNTVLESAAHMMELHAHQKVLLEVEYTEEVGTGLGPTLEFYTLVCHEFQRNGLGLWR 1194

Query: 1068 DDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKS 889
            +D ++  C   +EAEN G ++S +GLFPRPW            SEVIKKF LLGQIVAK+
Sbjct: 1195 EDPVN--CINAVEAENPGFLMSPVGLFPRPWSPSLSIESSTPFSEVIKKFRLLGQIVAKA 1252

Query: 888  LQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEK 709
            LQDGRVLDLPFS AFYKL+LGK+LT++DI+S DP+ G ALLEFQA VE++ YL+SL  E+
Sbjct: 1253 LQDGRVLDLPFSHAFYKLVLGKELTVHDIRSIDPSLGKALLEFQATVERKRYLKSLSEEE 1312

Query: 708  SHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTK 529
            S D  + L+ TKIEDLCLDFTLPGYPDY LV    + DS+MVNL N++EY+ L+VDATTK
Sbjct: 1313 SQDPGLCLQSTKIEDLCLDFTLPGYPDYDLVL---APDSKMVNLGNIEEYITLIVDATTK 1369

Query: 528  SGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVS 349
            SGI+RQVEAFKSGFDQV  ++HLK+FTEEELERLLCGEHVLWNS+ELLDHIKFDHGYT+S
Sbjct: 1370 SGISRQVEAFKSGFDQVLNVQHLKIFTEEELERLLCGEHVLWNSDELLDHIKFDHGYTIS 1429

Query: 348  SPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLP 169
            SPP+VNL+E+M+EFD +QQRAFLQFVTGAPRLP+GG ASLNPKLTIVRKHC   IDADLP
Sbjct: 1430 SPPVVNLIEVMREFDSEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHCGKWIDADLP 1489

Query: 168  SVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            SVMTCANYLKLPPYS+KE+MKEKLLYAI EGQGSFHLS
Sbjct: 1490 SVMTCANYLKLPPYSTKEIMKEKLLYAITEGQGSFHLS 1527


>ref|XP_020553308.1| E3 ubiquitin-protein ligase UPL4 isoform X3 [Sesamum indicum]
          Length = 1127

 Score = 1048 bits (2709), Expect = 0.0
 Identities = 528/700 (75%), Positives = 580/700 (82%)
 Frame = -2

Query: 2154 QEAKPNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDN 1975
            Q+ + NLL SSPA  SS +Q  +DSA VAD QTD +E +EH+PL+EDGG N DHPG SD 
Sbjct: 436  QDKESNLLLSSPADTSSSSQRIMDSADVADVQTDPLEPKEHDPLQEDGGTNFDHPGCSDC 495

Query: 1974 EDTSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKP 1795
            E+TS KLLFYLE QQLNC+LTLYQSIL  Q   + D I S S+W RI+K+TY+R + T+ 
Sbjct: 496  EETSPKLLFYLESQQLNCKLTLYQSILNLQTETDHDNISSASLWNRIYKLTYRRPVTTRV 555

Query: 1794 NHAKRIHDENLPSLLSKRASFFQYAPLFSPLFVSEVDFEKSDPAYDLLSLLRSLEGINRL 1615
             H K  HDE   SL  KRA FFQY P F P+F SEVD EK  P YD+LSLL+SLEGINRL
Sbjct: 556  RHPKPSHDEAQCSLSLKRALFFQYTPYFCPMFASEVDLEKLGPTYDILSLLKSLEGINRL 615

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
            RFHLMS ERTY F EG T DLDKLNV V+EVPPNEFVNKKLT+KL+QQMRDP AVSVGAM
Sbjct: 616  RFHLMSRERTYAFAEGRTDDLDKLNVVVSEVPPNEFVNKKLTEKLEQQMRDPMAVSVGAM 675

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            PAWCTQLM WCPFLFGFEARCKYFHLAALG+SP Q              RQQ+     R+
Sbjct: 676  PAWCTQLMAWCPFLFGFEARCKYFHLAALGRSPVQTHSVSHGNAGGSGGRQQS-----RR 730

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRNKILESAAQMMELH   KVL EVEY+EEVGTGLGPTLEFYTLVCHEFQRSGLGM
Sbjct: 731  KILVHRNKILESAAQMMELHTHQKVLFEVEYDEEVGTGLGPTLEFYTLVCHEFQRSGLGM 790

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WRDD + L C+  LE EN+G +VS  GLFPRPW            S+VI+KF+LLG IVA
Sbjct: 791  WRDDTVPLQCTAVLETENTGFLVSPFGLFPRPWSPSLSASSSSVYSDVIEKFSLLGYIVA 850

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLPFSKA YKLILGK+L+LYDIQSFDPA G ALLEFQA+VE+++YLRS+C 
Sbjct: 851  KALQDGRVLDLPFSKALYKLILGKELSLYDIQSFDPASGRALLEFQAVVERKEYLRSVCK 910

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            E+S DLDV LR+TKIEDLCLDFTLPGYPDY LV     +DSRMVNLYNLDEY+ L+VDAT
Sbjct: 911  EESADLDVCLRNTKIEDLCLDFTLPGYPDYVLV---PETDSRMVNLYNLDEYITLIVDAT 967

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
            TKSGIARQVEAFKSGFDQVFPI+HLKVFTEEELERLLCGEHVLWNSE+LLDHIKFDHGYT
Sbjct: 968  TKSGIARQVEAFKSGFDQVFPIKHLKVFTEEELERLLCGEHVLWNSEDLLDHIKFDHGYT 1027

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
            +SSPPIVNLLEIMQEFDL QQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCS  IDAD
Sbjct: 1028 ISSPPIVNLLEIMQEFDLKQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSKGIDAD 1087

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKEVMKEKLLYA+ EGQGSFHLS
Sbjct: 1088 LPSVMTCANYLKLPPYSSKEVMKEKLLYAVTEGQGSFHLS 1127


>ref|XP_019265055.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            attenuata]
 ref|XP_019265056.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            attenuata]
 gb|OIT35986.1| e3 ubiquitin-protein ligase upl4 [Nicotiana attenuata]
          Length = 1539

 Score =  949 bits (2452), Expect = 0.0
 Identities = 502/880 (57%), Positives = 623/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL    P  E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFALFGRLLLYNSVPPLEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTESVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T +     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQDASTSQGKSPGPMELDTTPT-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++   + +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAESLEKGKLCSSEDDSSTSLECTGCSDDEDVA 908

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+++H++TY+R +  KP  A 
Sbjct: 909  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSQVHRVTYRRFVRHKPGCAQ 968

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 969  SGKHVVDST-PS--EKPITWWQYTPYFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1025

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   +    +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1026 SFHLMSRMKIYAFAEGKTTDFGDIKFTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1085

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA G+ P Q              R QN+G L RK
Sbjct: 1086 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGKPPIQPEPSSHNTAGGMSGRHQNNGGLRRK 1145

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1146 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1205

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1206 WRGDRMASGTMS--VEEESGMLFSSFGLFPRPWSPLSHSSSGLEFSEVLKKFVLLGQIVA 1263

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1264 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGVALLEFQALIERKRHLESE-G 1322

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1323 KSSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1379

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL+VFTE+ELERLLCGE  LWNS ELLDHIKFDHGYT
Sbjct: 1380 LNSGISRQIGAFKSGFDQVFPIKHLQVFTEDELERLLCGECGLWNSNELLDHIKFDHGYT 1439

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1440 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSVWVDAD 1499

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1500 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1539


>ref|XP_009622740.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            tomentosiformis]
 ref|XP_009622741.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            tomentosiformis]
 ref|XP_016461945.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X1
            [Nicotiana tabacum]
 ref|XP_016461946.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X1
            [Nicotiana tabacum]
 ref|XP_018632466.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            tomentosiformis]
          Length = 1541

 Score =  947 bits (2448), Expect = 0.0
 Identities = 501/881 (56%), Positives = 626/881 (71%), Gaps = 8/881 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRFE+FGRLLL    P  E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFELFGRLLLYNSVPPLEDSTFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKVQFV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVQFVKGDGESSLGDYTECVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL ++D                       D T++     +  + QE K
Sbjct: 794  VSRRKSEKLKPPTLATEDESSSRSSQDVSTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S  A +    +   DS  +++   + +E+ +    ++D   ++   G SD+ED +
Sbjct: 849  SNLQLSVEAESMDIEKTKSDSMDISNINAESLEKGKLCSSEDDSSTSLGCTGCSDDEDVA 908

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAK 1783
             KL+FYLEGQQLN +LTLYQ++L QQ     DII ++S+W+++H++TY+R +  KP  A+
Sbjct: 909  PKLIFYLEGQQLNQKLTLYQTVLHQQIKAGNDIITNSSMWSQVHRVTYRRCVRHKPGCAQ 968

Query: 1782 RIHD--ENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRLR 1612
                  ++ PS   K   ++QY P FS +F  E VD EKS P Y++L LL+SLEG+NR  
Sbjct: 969  SCKHVVDSTPS--GKPIMWWQYTPFFSSMFSCEMVDLEKSSPTYEILFLLKSLEGMNRFS 1026

Query: 1611 FHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMP 1432
            FHLMSH + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VS+G MP
Sbjct: 1027 FHLMSHIKIYAFAEGKTTDFSDIKVTNSDLPQNEFANSKLTEKLELQMRNPFSVSIGGMP 1086

Query: 1431 AWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKK 1252
             WC QL++ CPFLFGFEARCKYF LAA GQ P                R QN+G L RKK
Sbjct: 1087 PWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIHPEPSSHNTAGGMSGRHQNNGGLRRKK 1146

Query: 1251 VLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMW 1072
            +LVHRN+IL+SA QMM+LH   KV+ EVEY++EVGTGLGPTLEF+TLV HEFQ+ GLGMW
Sbjct: 1147 ILVHRNRILDSATQMMDLHADQKVVIEVEYSDEVGTGLGPTLEFFTLVGHEFQKIGLGMW 1206

Query: 1071 RDDCISLHCSEDLEAEN-SGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            R D ++   S  +  E  SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1207 RGDSMA---SGTMSVEQESGMLFSSFGLFPRPWSPLSRSLSGLEFSEVLKKFGLLGQIVA 1263

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLC- 718
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSF+P  G ALLEFQA+VE++ +L SL  
Sbjct: 1264 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFEPELGRALLEFQALVERKRHLESLSE 1323

Query: 717  GEKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDA 538
            G+ S DL++   +TKI+DL LD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA
Sbjct: 1324 GKPSLDLELNFGNTKIDDLYLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDA 1380

Query: 537  TTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGY 358
            +  SGI+RQ+ AFKSGFDQVFPI+HL+VFTE+ELERLLCGE   WNS ELLDHIKFDHGY
Sbjct: 1381 SLNSGISRQIGAFKSGFDQVFPIKHLQVFTEDELERLLCGECGFWNSNELLDHIKFDHGY 1440

Query: 357  TVSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDA 178
            T SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DA
Sbjct: 1441 TASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDA 1500

Query: 177  DLPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            DLPSVMTCANYLKLPPYSSK  MKEKLLYAI EGQGSF+LS
Sbjct: 1501 DLPSVMTCANYLKLPPYSSKGKMKEKLLYAITEGQGSFYLS 1541


>ref|XP_019265058.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X2 [Nicotiana
            attenuata]
          Length = 1538

 Score =  944 bits (2440), Expect = 0.0
 Identities = 502/880 (57%), Positives = 622/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL    P  E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFALFGRLLLYNSVPPLEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTESVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T +     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQDASTSQGKSPGPMELDTTPT-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++     +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAS-LEKGKLCSSEDDSSTSLECTGCSDDEDVA 907

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+++H++TY+R +  KP  A 
Sbjct: 908  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSQVHRVTYRRFVRHKPGCAQ 967

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 968  SGKHVVDST-PS--EKPITWWQYTPYFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1024

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   +    +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1025 SFHLMSRMKIYAFAEGKTTDFGDIKFTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1084

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA G+ P Q              R QN+G L RK
Sbjct: 1085 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGKPPIQPEPSSHNTAGGMSGRHQNNGGLRRK 1144

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1145 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1204

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1205 WRGDRMASGTMS--VEEESGMLFSSFGLFPRPWSPLSHSSSGLEFSEVLKKFVLLGQIVA 1262

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1263 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGVALLEFQALIERKRHLESE-G 1321

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1322 KSSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1378

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL+VFTE+ELERLLCGE  LWNS ELLDHIKFDHGYT
Sbjct: 1379 LNSGISRQIGAFKSGFDQVFPIKHLQVFTEDELERLLCGECGLWNSNELLDHIKFDHGYT 1438

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1439 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSVWVDAD 1498

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1499 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1538


>ref|XP_016503890.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X1
            [Nicotiana tabacum]
 ref|XP_016503897.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X1
            [Nicotiana tabacum]
 ref|XP_016503904.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X1
            [Nicotiana tabacum]
          Length = 1539

 Score =  944 bits (2440), Expect = 0.0
 Identities = 499/880 (56%), Positives = 620/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL       E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFALFGRLLLYNSVCPPEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTEGVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T++     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQDASTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++   + +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAESLEKGKLCSSEDDSSTSLECTGCSDDEDVA 908

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+ +H++ Y+R +  KP  A 
Sbjct: 909  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSHVHRVIYRRFVRHKPGCAQ 968

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 969  SGKHVVDST-PS--EKPITWWQYTPFFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1025

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1026 SFHLMSRRKIYAFAEGKTTDFGDIKVTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1085

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA GQ P Q              R QN+  L RK
Sbjct: 1086 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIQPEPSSHNMAGGMSGRHQNNSGLRRK 1145

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1146 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1205

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1206 WRGDRMASGTVS--VEEESGMLFSSFGLFPRPWSPLSRSSSGLEFSEVLKKFVLLGQIVA 1263

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1264 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGGALLEFQALIERKRHLESE-G 1322

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1323 KPSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1379

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL++FTE+ELERLLCGE   WNS ELLDHIKFDHGYT
Sbjct: 1380 LNSGISRQIGAFKSGFDQVFPIKHLQIFTEDELERLLCGECGFWNSNELLDHIKFDHGYT 1439

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1440 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDAD 1499

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1500 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1539


>ref|XP_009794383.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Nicotiana
            sylvestris]
          Length = 1539

 Score =  944 bits (2439), Expect = 0.0
 Identities = 499/880 (56%), Positives = 620/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL       E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFVLFGRLLLYNSVCPPEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTEGVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T++     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQGASTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++   + +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAESLEKGKLCSSEDDSSTSLECTGCSDDEDVA 908

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+ +H++ Y+R +  KP  A 
Sbjct: 909  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSHVHRVIYRRFVRHKPGCAQ 968

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 969  SGKHVVDST-PS--EKPITWWQYTPFFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1025

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1026 SFHLMSRRKIYAFAEGKTTDFGDIKVTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1085

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA GQ P Q              R QN+  L RK
Sbjct: 1086 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIQPEPSSHNTAGGMSGRHQNNSGLRRK 1145

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1146 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1205

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1206 WRGDRMASGTVS--VEEESGMLFSSFGLFPRPWSPLSRSSSGLEFSEVLKKFVLLGQIVA 1263

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1264 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGGALLEFQALIERKRHLESE-G 1322

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1323 KPSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1379

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL++FTE+ELERLLCGE   WNS ELLDHIKFDHGYT
Sbjct: 1380 LNSGISRQIGAFKSGFDQVFPIKHLQIFTEDELERLLCGECGFWNSNELLDHIKFDHGYT 1439

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1440 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDAD 1499

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1500 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1539


>ref|XP_009622742.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X2 [Nicotiana
            tomentosiformis]
 ref|XP_016461947.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X2
            [Nicotiana tabacum]
          Length = 1540

 Score =  942 bits (2436), Expect = 0.0
 Identities = 501/881 (56%), Positives = 625/881 (70%), Gaps = 8/881 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRFE+FGRLLL    P  E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFELFGRLLLYNSVPPLEDSTFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKVQFV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVQFVKGDGESSLGDYTECVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL ++D                       D T++     +  + QE K
Sbjct: 794  VSRRKSEKLKPPTLATEDESSSRSSQDVSTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S  A +    +   DS  +++     +E+ +    ++D   ++   G SD+ED +
Sbjct: 849  SNLQLSVEAESMDIEKTKSDSMDISNINAS-LEKGKLCSSEDDSSTSLGCTGCSDDEDVA 907

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAK 1783
             KL+FYLEGQQLN +LTLYQ++L QQ     DII ++S+W+++H++TY+R +  KP  A+
Sbjct: 908  PKLIFYLEGQQLNQKLTLYQTVLHQQIKAGNDIITNSSMWSQVHRVTYRRCVRHKPGCAQ 967

Query: 1782 RIHD--ENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRLR 1612
                  ++ PS   K   ++QY P FS +F  E VD EKS P Y++L LL+SLEG+NR  
Sbjct: 968  SCKHVVDSTPS--GKPIMWWQYTPFFSSMFSCEMVDLEKSSPTYEILFLLKSLEGMNRFS 1025

Query: 1611 FHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMP 1432
            FHLMSH + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VS+G MP
Sbjct: 1026 FHLMSHIKIYAFAEGKTTDFSDIKVTNSDLPQNEFANSKLTEKLELQMRNPFSVSIGGMP 1085

Query: 1431 AWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKK 1252
             WC QL++ CPFLFGFEARCKYF LAA GQ P                R QN+G L RKK
Sbjct: 1086 PWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIHPEPSSHNTAGGMSGRHQNNGGLRRKK 1145

Query: 1251 VLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMW 1072
            +LVHRN+IL+SA QMM+LH   KV+ EVEY++EVGTGLGPTLEF+TLV HEFQ+ GLGMW
Sbjct: 1146 ILVHRNRILDSATQMMDLHADQKVVIEVEYSDEVGTGLGPTLEFFTLVGHEFQKIGLGMW 1205

Query: 1071 RDDCISLHCSEDLEAEN-SGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            R D ++   S  +  E  SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1206 RGDSMA---SGTMSVEQESGMLFSSFGLFPRPWSPLSRSLSGLEFSEVLKKFGLLGQIVA 1262

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLC- 718
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSF+P  G ALLEFQA+VE++ +L SL  
Sbjct: 1263 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFEPELGRALLEFQALVERKRHLESLSE 1322

Query: 717  GEKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDA 538
            G+ S DL++   +TKI+DL LD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA
Sbjct: 1323 GKPSLDLELNFGNTKIDDLYLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDA 1379

Query: 537  TTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGY 358
            +  SGI+RQ+ AFKSGFDQVFPI+HL+VFTE+ELERLLCGE   WNS ELLDHIKFDHGY
Sbjct: 1380 SLNSGISRQIGAFKSGFDQVFPIKHLQVFTEDELERLLCGECGFWNSNELLDHIKFDHGY 1439

Query: 357  TVSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDA 178
            T SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DA
Sbjct: 1440 TASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDA 1499

Query: 177  DLPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            DLPSVMTCANYLKLPPYSSK  MKEKLLYAI EGQGSF+LS
Sbjct: 1500 DLPSVMTCANYLKLPPYSSKGKMKEKLLYAITEGQGSFYLS 1540


>ref|XP_019077234.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X4 [Vitis
            vinifera]
          Length = 1334

 Score =  942 bits (2434), Expect = 0.0
 Identities = 505/877 (57%), Positives = 617/877 (70%), Gaps = 9/877 (1%)
 Frame = -2

Query: 2658 ANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHTYKSRN 2479
            ++H   +EKRFE+FG LLLS  +P  E+ PL  LI++LQ ALSSVENFP+I SH  K RN
Sbjct: 470  SSHYDNVEKRFEVFGGLLLSLSEPLSEDLPLSVLIQKLQHALSSVENFPVILSHASKQRN 529

Query: 2478 SYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPRVS--- 2308
            S+ATVP GRC S+PCLKV+F +E  E  L DY+EDV+ VDPF  LD I  +LW +VS   
Sbjct: 530  SFATVPNGRCVSHPCLKVRFTKEEVETSLYDYSEDVLTVDPFSSLDAIEGFLWRKVSIKR 589

Query: 2307 NDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAKPNLLF 2128
             + T ++   S D K                     D+  S+ M  +  + QE K +   
Sbjct: 590  TEPTNSVFQASHDMK----GPIFQGPLDAGSQGKSPDLMESESMSSEFPEVQEDKDSSQ- 644

Query: 2127 SSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDH--PGFSDNEDTSTKL 1954
            S+P  AS+  ++T   A  + G+T  V  E+   +  + G  M    P     ED S KL
Sbjct: 645  STPESASNLREMTPGEAT-SSGETQTVSAEQEQHVSSEAGVKMKTQCPESCSGEDASVKL 703

Query: 1953 LFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAKRIH 1774
            LFYLEGQQLN ELT+YQ+I++QQ   E +IIPS  +W ++H +TY+  +  K  H +   
Sbjct: 704  LFYLEGQQLNRELTMYQAIIQQQIEAEHEIIPSGKLWGQVHTLTYRAAVEPKQTHPQECL 763

Query: 1773 DENLPSLLSKRASFFQYAPLFSPLFVSEV--DFEKSDPAYDLLSLLRSLEGINRLRFHLM 1600
             +N P + +K  +  Q AP FS +FV E+  + +KS P YD+L LL+SLEG+N+ +FHLM
Sbjct: 764  -QNSP-VSAKVGTHLQQAPFFSNIFVPELVAELDKSGPTYDILFLLKSLEGMNKFKFHLM 821

Query: 1599 SHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPAWCT 1420
            S ERT  F EG   +LD L VAV  +P NEFVN KLT+KL+QQMRDP AVS+G MP WC 
Sbjct: 822  SRERTKAFAEGRIDNLDNLKVAVPVIPENEFVNSKLTEKLEQQMRDPLAVSIGGMPLWCN 881

Query: 1419 QLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKVLVH 1240
            QLM   PFLFGFEARCKYF LAA G   +Q              R+ N G LPRKK LV 
Sbjct: 882  QLMALYPFLFGFEARCKYFRLAAFGPLQAQPHSSFHNTSGAPSDRRHNAGSLPRKKFLVC 941

Query: 1239 RNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRDDC 1060
            R++IL+SAAQMM LH   KV+ EVEYNEEVGTGLGPTLEFYTLVCHEFQ++GLGMWR+D 
Sbjct: 942  RDRILDSAAQMMNLHACQKVVLEVEYNEEVGTGLGPTLEFYTLVCHEFQKTGLGMWREDY 1001

Query: 1059 ISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKSLQD 880
             S    + L+A  SG++VS  GLFPRPW            S+V K+F LLGQ+VAK+LQD
Sbjct: 1002 TSSTSCKSLQA-GSGMVVSPSGLFPRPWSSTLSTSNGIEFSDVTKQFVLLGQVVAKALQD 1060

Query: 879  GRVLDLPFSKAFYKL-ILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEKS- 706
            GRVLDLPFSKAFYKL ILG++L++YDIQSFDP  G  LLEFQA+++++ YL ++CGEKS 
Sbjct: 1061 GRVLDLPFSKAFYKLAILGQELSVYDIQSFDPELGRVLLEFQALIDRKRYLETVCGEKST 1120

Query: 705  HDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTKS 526
             D+D+  R+TKIEDL LDFTLPGYP+Y L SGS   D +MV + NL+EYV L+VD T  +
Sbjct: 1121 FDVDMCFRNTKIEDLYLDFTLPGYPEYVLTSGS---DHKMVTMTNLEEYVSLLVDTTINA 1177

Query: 525  GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVSS 346
            GI+RQVEAF+SGF+QVFPI+HL++FTEEELE+LLCGE   W    LLDHIKFDHGYT SS
Sbjct: 1178 GISRQVEAFRSGFNQVFPIKHLQIFTEEELEKLLCGERDSWACNGLLDHIKFDHGYTASS 1237

Query: 345  PPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLPS 166
            PPI+NLLEI+QEFD +Q+RAFLQFVTGAPRLP GGLASLNPKLTIVRKHCS   DADLPS
Sbjct: 1238 PPIINLLEIVQEFDHEQRRAFLQFVTGAPRLPPGGLASLNPKLTIVRKHCSKWADADLPS 1297

Query: 165  VMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            VMTCANYLKLPPYSSKE MKEKLLYAI EGQGSFHLS
Sbjct: 1298 VMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFHLS 1334


>ref|XP_019077233.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X3 [Vitis
            vinifera]
          Length = 1526

 Score =  942 bits (2434), Expect = 0.0
 Identities = 505/877 (57%), Positives = 617/877 (70%), Gaps = 9/877 (1%)
 Frame = -2

Query: 2658 ANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHTYKSRN 2479
            ++H   +EKRFE+FG LLLS  +P  E+ PL  LI++LQ ALSSVENFP+I SH  K RN
Sbjct: 662  SSHYDNVEKRFEVFGGLLLSLSEPLSEDLPLSVLIQKLQHALSSVENFPVILSHASKQRN 721

Query: 2478 SYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPRVS--- 2308
            S+ATVP GRC S+PCLKV+F +E  E  L DY+EDV+ VDPF  LD I  +LW +VS   
Sbjct: 722  SFATVPNGRCVSHPCLKVRFTKEEVETSLYDYSEDVLTVDPFSSLDAIEGFLWRKVSIKR 781

Query: 2307 NDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAKPNLLF 2128
             + T ++   S D K                     D+  S+ M  +  + QE K +   
Sbjct: 782  TEPTNSVFQASHDMK----GPIFQGPLDAGSQGKSPDLMESESMSSEFPEVQEDKDSSQ- 836

Query: 2127 SSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDH--PGFSDNEDTSTKL 1954
            S+P  AS+  ++T   A  + G+T  V  E+   +  + G  M    P     ED S KL
Sbjct: 837  STPESASNLREMTPGEAT-SSGETQTVSAEQEQHVSSEAGVKMKTQCPESCSGEDASVKL 895

Query: 1953 LFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAKRIH 1774
            LFYLEGQQLN ELT+YQ+I++QQ   E +IIPS  +W ++H +TY+  +  K  H +   
Sbjct: 896  LFYLEGQQLNRELTMYQAIIQQQIEAEHEIIPSGKLWGQVHTLTYRAAVEPKQTHPQECL 955

Query: 1773 DENLPSLLSKRASFFQYAPLFSPLFVSEV--DFEKSDPAYDLLSLLRSLEGINRLRFHLM 1600
             +N P + +K  +  Q AP FS +FV E+  + +KS P YD+L LL+SLEG+N+ +FHLM
Sbjct: 956  -QNSP-VSAKVGTHLQQAPFFSNIFVPELVAELDKSGPTYDILFLLKSLEGMNKFKFHLM 1013

Query: 1599 SHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPAWCT 1420
            S ERT  F EG   +LD L VAV  +P NEFVN KLT+KL+QQMRDP AVS+G MP WC 
Sbjct: 1014 SRERTKAFAEGRIDNLDNLKVAVPVIPENEFVNSKLTEKLEQQMRDPLAVSIGGMPLWCN 1073

Query: 1419 QLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKVLVH 1240
            QLM   PFLFGFEARCKYF LAA G   +Q              R+ N G LPRKK LV 
Sbjct: 1074 QLMALYPFLFGFEARCKYFRLAAFGPLQAQPHSSFHNTSGAPSDRRHNAGSLPRKKFLVC 1133

Query: 1239 RNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRDDC 1060
            R++IL+SAAQMM LH   KV+ EVEYNEEVGTGLGPTLEFYTLVCHEFQ++GLGMWR+D 
Sbjct: 1134 RDRILDSAAQMMNLHACQKVVLEVEYNEEVGTGLGPTLEFYTLVCHEFQKTGLGMWREDY 1193

Query: 1059 ISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKSLQD 880
             S    + L+A  SG++VS  GLFPRPW            S+V K+F LLGQ+VAK+LQD
Sbjct: 1194 TSSTSCKSLQA-GSGMVVSPSGLFPRPWSSTLSTSNGIEFSDVTKQFVLLGQVVAKALQD 1252

Query: 879  GRVLDLPFSKAFYKL-ILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEKS- 706
            GRVLDLPFSKAFYKL ILG++L++YDIQSFDP  G  LLEFQA+++++ YL ++CGEKS 
Sbjct: 1253 GRVLDLPFSKAFYKLAILGQELSVYDIQSFDPELGRVLLEFQALIDRKRYLETVCGEKST 1312

Query: 705  HDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTKS 526
             D+D+  R+TKIEDL LDFTLPGYP+Y L SGS   D +MV + NL+EYV L+VD T  +
Sbjct: 1313 FDVDMCFRNTKIEDLYLDFTLPGYPEYVLTSGS---DHKMVTMTNLEEYVSLLVDTTINA 1369

Query: 525  GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVSS 346
            GI+RQVEAF+SGF+QVFPI+HL++FTEEELE+LLCGE   W    LLDHIKFDHGYT SS
Sbjct: 1370 GISRQVEAFRSGFNQVFPIKHLQIFTEEELEKLLCGERDSWACNGLLDHIKFDHGYTASS 1429

Query: 345  PPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLPS 166
            PPI+NLLEI+QEFD +Q+RAFLQFVTGAPRLP GGLASLNPKLTIVRKHCS   DADLPS
Sbjct: 1430 PPIINLLEIVQEFDHEQRRAFLQFVTGAPRLPPGGLASLNPKLTIVRKHCSKWADADLPS 1489

Query: 165  VMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            VMTCANYLKLPPYSSKE MKEKLLYAI EGQGSFHLS
Sbjct: 1490 VMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFHLS 1526


>ref|XP_010653449.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Vitis
            vinifera]
          Length = 1579

 Score =  942 bits (2434), Expect = 0.0
 Identities = 505/877 (57%), Positives = 617/877 (70%), Gaps = 9/877 (1%)
 Frame = -2

Query: 2658 ANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHTYKSRN 2479
            ++H   +EKRFE+FG LLLS  +P  E+ PL  LI++LQ ALSSVENFP+I SH  K RN
Sbjct: 715  SSHYDNVEKRFEVFGGLLLSLSEPLSEDLPLSVLIQKLQHALSSVENFPVILSHASKQRN 774

Query: 2478 SYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPRVS--- 2308
            S+ATVP GRC S+PCLKV+F +E  E  L DY+EDV+ VDPF  LD I  +LW +VS   
Sbjct: 775  SFATVPNGRCVSHPCLKVRFTKEEVETSLYDYSEDVLTVDPFSSLDAIEGFLWRKVSIKR 834

Query: 2307 NDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAKPNLLF 2128
             + T ++   S D K                     D+  S+ M  +  + QE K +   
Sbjct: 835  TEPTNSVFQASHDMK----GPIFQGPLDAGSQGKSPDLMESESMSSEFPEVQEDKDSSQ- 889

Query: 2127 SSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDH--PGFSDNEDTSTKL 1954
            S+P  AS+  ++T   A  + G+T  V  E+   +  + G  M    P     ED S KL
Sbjct: 890  STPESASNLREMTPGEAT-SSGETQTVSAEQEQHVSSEAGVKMKTQCPESCSGEDASVKL 948

Query: 1953 LFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHAKRIH 1774
            LFYLEGQQLN ELT+YQ+I++QQ   E +IIPS  +W ++H +TY+  +  K  H +   
Sbjct: 949  LFYLEGQQLNRELTMYQAIIQQQIEAEHEIIPSGKLWGQVHTLTYRAAVEPKQTHPQECL 1008

Query: 1773 DENLPSLLSKRASFFQYAPLFSPLFVSEV--DFEKSDPAYDLLSLLRSLEGINRLRFHLM 1600
             +N P + +K  +  Q AP FS +FV E+  + +KS P YD+L LL+SLEG+N+ +FHLM
Sbjct: 1009 -QNSP-VSAKVGTHLQQAPFFSNIFVPELVAELDKSGPTYDILFLLKSLEGMNKFKFHLM 1066

Query: 1599 SHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPAWCT 1420
            S ERT  F EG   +LD L VAV  +P NEFVN KLT+KL+QQMRDP AVS+G MP WC 
Sbjct: 1067 SRERTKAFAEGRIDNLDNLKVAVPVIPENEFVNSKLTEKLEQQMRDPLAVSIGGMPLWCN 1126

Query: 1419 QLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKVLVH 1240
            QLM   PFLFGFEARCKYF LAA G   +Q              R+ N G LPRKK LV 
Sbjct: 1127 QLMALYPFLFGFEARCKYFRLAAFGPLQAQPHSSFHNTSGAPSDRRHNAGSLPRKKFLVC 1186

Query: 1239 RNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRDDC 1060
            R++IL+SAAQMM LH   KV+ EVEYNEEVGTGLGPTLEFYTLVCHEFQ++GLGMWR+D 
Sbjct: 1187 RDRILDSAAQMMNLHACQKVVLEVEYNEEVGTGLGPTLEFYTLVCHEFQKTGLGMWREDY 1246

Query: 1059 ISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKSLQD 880
             S    + L+A  SG++VS  GLFPRPW            S+V K+F LLGQ+VAK+LQD
Sbjct: 1247 TSSTSCKSLQA-GSGMVVSPSGLFPRPWSSTLSTSNGIEFSDVTKQFVLLGQVVAKALQD 1305

Query: 879  GRVLDLPFSKAFYKL-ILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCGEKS- 706
            GRVLDLPFSKAFYKL ILG++L++YDIQSFDP  G  LLEFQA+++++ YL ++CGEKS 
Sbjct: 1306 GRVLDLPFSKAFYKLAILGQELSVYDIQSFDPELGRVLLEFQALIDRKRYLETVCGEKST 1365

Query: 705  HDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTKS 526
             D+D+  R+TKIEDL LDFTLPGYP+Y L SGS   D +MV + NL+EYV L+VD T  +
Sbjct: 1366 FDVDMCFRNTKIEDLYLDFTLPGYPEYVLTSGS---DHKMVTMTNLEEYVSLLVDTTINA 1422

Query: 525  GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVSS 346
            GI+RQVEAF+SGF+QVFPI+HL++FTEEELE+LLCGE   W    LLDHIKFDHGYT SS
Sbjct: 1423 GISRQVEAFRSGFNQVFPIKHLQIFTEEELEKLLCGERDSWACNGLLDHIKFDHGYTASS 1482

Query: 345  PPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLPS 166
            PPI+NLLEI+QEFD +Q+RAFLQFVTGAPRLP GGLASLNPKLTIVRKHCS   DADLPS
Sbjct: 1483 PPIINLLEIVQEFDHEQRRAFLQFVTGAPRLPPGGLASLNPKLTIVRKHCSKWADADLPS 1542

Query: 165  VMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            VMTCANYLKLPPYSSKE MKEKLLYAI EGQGSFHLS
Sbjct: 1543 VMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFHLS 1579


>gb|PHU06662.1| E3 ubiquitin-protein ligase UPL4 [Capsicum chinense]
          Length = 1555

 Score =  941 bits (2433), Expect = 0.0
 Identities = 496/877 (56%), Positives = 620/877 (70%), Gaps = 4/877 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + N L I+EKRFE+FGRLLL    P+ E+   + L+RRL SAL SVENFP+I SH 
Sbjct: 688  DGDGSLNQLYIVEKRFELFGRLLLYNSGPSLEDSSFLALVRRLHSALCSVENFPVILSHA 747

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYAT+PYG+CTSYPCLKVQFV+ +GE  L DY E+VVNVDPF PL+ I RYLWP+
Sbjct: 748  SKLRNSYATIPYGQCTSYPCLKVQFVKGDGESLLGDYTENVVNVDPFSPLETIERYLWPK 807

Query: 2313 VSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAKPNL 2134
            VS  K++ L   + D +E                        S     D  + QE K NL
Sbjct: 808  VSRKKSEKLNPSTVDLEEESPSRVSQDVSTSQGKNPVP--MESDTTSTDSHEIQEVKNNL 865

Query: 2133 LFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTSTKL 1954
               +        Q   D   ++D   +F+E+ + N  + D   ++   G  D+E+ + KL
Sbjct: 866  QLFAEVEIVDIEQRKSDPMDISDVNAEFLEKGKLNSSEVDSSTSLVCTGCCDDENIAPKL 925

Query: 1953 LFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPN--HAKR 1780
            +FYL+GQ+LN +L+LYQ++L+QQ   E DII ++S+W+++H++T++R +  KP   H+ +
Sbjct: 926  IFYLDGQKLNHKLSLYQAVLQQQIKAENDIITNSSMWSQVHRVTFRRFVRHKPGCPHSCK 985

Query: 1779 IHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRLRFHL 1603
                  PS   K  +++QY P FS LF SE VD EKS P YD+L LLRSLEG+NR  FHL
Sbjct: 986  HAVHYTPS--EKPTAWWQYTPSFSSLFGSEMVDLEKSSPTYDILFLLRSLEGLNRFSFHL 1043

Query: 1602 MSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAMPAWC 1423
             S  +   F EG T++L  + V  +++P NEF + KLT+KL+ QMR+P +VS+G MPAWC
Sbjct: 1044 GSRTKINAFAEGKTTNLGDIKVTNSDLPQNEFASTKLTEKLELQMRNPFSVSIGGMPAWC 1103

Query: 1422 TQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRKKVLV 1243
             QL++ CPFLFGF++RCKYF L A G+   Q              R QN   + RKK LV
Sbjct: 1104 GQLVNSCPFLFGFDSRCKYFRLVAFGRQSIQPESSSHNTAAGTNGRHQNSSGIRRKKFLV 1163

Query: 1242 HRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGMWRDD 1063
            HR+ IL+SA QMM+LH   KV+ EVEYN+EVGTGLGPTLEF+TLV HEFQ+ GLGMWR D
Sbjct: 1164 HRSSILDSARQMMDLHANQKVVIEVEYNDEVGTGLGPTLEFFTLVSHEFQKIGLGMWRGD 1223

Query: 1062 CISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVAKSLQ 883
             ++ H S  +E + SG+  S LGLFPRPW            SEV+K+F LLGQIVAKSLQ
Sbjct: 1224 HMA-HGSVSVE-DKSGITFSPLGLFPRPWSPSAHSLSGLEFSEVLKRFVLLGQIVAKSLQ 1281

Query: 882  DGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLC-GEKS 706
            DGRVLDL  SKAFYKLILGK+LT+YDIQSFDP  G  LLEFQA+VE++ ++ S C G+ S
Sbjct: 1282 DGRVLDLRLSKAFYKLILGKELTVYDIQSFDPELGGVLLEFQALVERKRHMESFCDGKSS 1341

Query: 705  HDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDATTKS 526
             DL++  R+TKI DLCLD+TLPGYPDY L S   +SD++MV+  NL+EYV+L+VDAT  S
Sbjct: 1342 LDLELNFRNTKISDLCLDYTLPGYPDYVLNS---ASDAKMVDSSNLEEYVLLIVDATLNS 1398

Query: 525  GIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYTVSS 346
            GI+RQ+ AFKSGFDQVFPIRHL+VFTE+ELERLLCGE   WNS ELLDHIKFDHGYT +S
Sbjct: 1399 GISRQIGAFKSGFDQVFPIRHLQVFTEDELERLLCGECGFWNSNELLDHIKFDHGYTANS 1458

Query: 345  PPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDADLPS 166
            PP++NLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASLNPKLTIVRK CS  +DA+LPS
Sbjct: 1459 PPVINLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLNPKLTIVRKSCSVWVDAELPS 1518

Query: 165  VMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            VMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1519 VMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFYLS 1555


>ref|XP_019191349.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Ipomoea nil]
 ref|XP_019191350.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Ipomoea nil]
 ref|XP_019191351.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X1 [Ipomoea nil]
          Length = 1558

 Score =  939 bits (2428), Expect = 0.0
 Identities = 508/885 (57%), Positives = 621/885 (70%), Gaps = 7/885 (0%)
 Frame = -2

Query: 2688 HLVGKEDNNAANHLRI-MEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFP 2512
            +L+GK +N  A   +  +EKRFE+FGRLLLS  DP+ E+ P ++LI +LQSALSSVENFP
Sbjct: 680  YLIGKTENVGALFWQYSIEKRFEVFGRLLLSSSDPSFEDLPFLSLIHQLQSALSSVENFP 739

Query: 2511 IISSHTYKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIG 2332
            +ISSHT + RNSYATVPYGRCTSYPCLKVQFV+  GE  L DY +DVVNVDPF  LD I 
Sbjct: 740  VISSHTSRLRNSYATVPYGRCTSYPCLKVQFVKGEGETDLCDYLKDVVNVDPFSSLDTIE 799

Query: 2331 RYLWPRVSNDKTKNLTLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQ 2152
             YL P+V N KT+     S+ + E +                  D      +  DV + Q
Sbjct: 800  GYLSPKVRNKKTQRSKSPSQ-AMEEEGSASQVSPDLSPSQVQTPDAIELDSIRFDVNQVQ 858

Query: 2151 EAKP---NLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFS 1981
              K    ++  S+P   ++  Q   +   + D   + + Q +   L E+     + P  S
Sbjct: 859  LPKREGHDIASSAPVEIANTEQANTEPMDITDVHMESLGQRQLR-LSEENSIITECPQCS 917

Query: 1980 DNEDTSTKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMAT 1801
            + E +S KLLFYLEG++LN  LTLYQ+IL+QQ   E D I +  VW ++H+ITYK  +  
Sbjct: 918  NEEGSSMKLLFYLEGKKLNNTLTLYQAILQQQIEAENDAITNAKVWNQVHRITYKSSVRG 977

Query: 1800 KPNHAKRI-HDENLPSLLSKRASFFQYAPLFSPLFVSEV-DFEKSDPAYDLLSLLRSLEG 1627
            K + A++  H+ +  +L  K   + QY P FS  F S V D ++S+P YD+LSLLRSLE 
Sbjct: 978  KQSCAQKCNHETHHSALHDKHTPWQQYTPFFSNTFASAVVDIDRSNPTYDILSLLRSLEQ 1037

Query: 1626 INRLRFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVS 1447
            +NR RFHL+S ER Y F E  T+DLD L      VP  EFVN KLTDKL+QQMRDP AVS
Sbjct: 1038 MNRFRFHLISRERVYAFAEERTNDLDSLKFDYFFVPQAEFVNNKLTDKLEQQMRDPFAVS 1097

Query: 1446 VGAMPAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGY 1267
            VG MP WCTQLMD CPFLFGFEARCKYF LA LG  P Q              RQ  +G 
Sbjct: 1098 VGGMPYWCTQLMDSCPFLFGFEARCKYFRLAILGNQPVQQPQLPSNNSGGLNRRQPPNGG 1157

Query: 1266 LPRKKVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRS 1087
             PRK+ LVHR+KIL+SA+QMM+L+   KV+ EVEY+EEVGTGLGPTLEFYTLV HEFQ+S
Sbjct: 1158 FPRKRFLVHRDKILDSASQMMDLYASQKVVIEVEYSEEVGTGLGPTLEFYTLVSHEFQKS 1217

Query: 1086 GLGMWRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLG 907
            GLGMWR DC+S   +  L  +  G +    GLFPRPW            SEV+KKF LLG
Sbjct: 1218 GLGMWRGDCMSSG-NISLVDDAHGSLSFAFGLFPRPWSQSVSALGKIEFSEVLKKFVLLG 1276

Query: 906  QIVAKSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLR 727
            QIVAKSLQDGRVLDLPFSKAFYKLILGK+L++YDIQSFDP  G  LLEF A+VE++ ++ 
Sbjct: 1277 QIVAKSLQDGRVLDLPFSKAFYKLILGKELSIYDIQSFDPELGRTLLEFHALVERKKHVE 1336

Query: 726  SLCGEKSHDL-DVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVML 550
            SL   KS  + ++  R+TKIEDLCLDF+LPGYPDY L S   + DS  V+L  L+EYV L
Sbjct: 1337 SLRERKSTLMPELYFRNTKIEDLCLDFSLPGYPDYILTS---TPDSETVDLSYLEEYVSL 1393

Query: 549  MVDATTKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKF 370
            +V+A+  SG++RQVE+FKSGFDQVFPI+HL++F+EEELERLLCGE  +WNS EL+DHIKF
Sbjct: 1394 IVNASIGSGVSRQVESFKSGFDQVFPIKHLQIFSEEELERLLCGEREIWNSNELVDHIKF 1453

Query: 369  DHGYTVSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSN 190
            DHGYT SS P +NLLEI+QEF++++QRAFLQFVTGAPRLP GGLASLNPKLTIVRK CS 
Sbjct: 1454 DHGYTASSSPAMNLLEIIQEFNIEKQRAFLQFVTGAPRLPPGGLASLNPKLTIVRKKCSK 1513

Query: 189  AIDADLPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
              D DLPSVMTCANYLKLPPY++KE+MKEKLLYAI EGQGSF+LS
Sbjct: 1514 WADDDLPSVMTCANYLKLPPYTTKEIMKEKLLYAITEGQGSFYLS 1558


>ref|XP_016503911.1| PREDICTED: E3 ubiquitin-protein ligase UPL4-like isoform X2
            [Nicotiana tabacum]
          Length = 1538

 Score =  939 bits (2428), Expect = 0.0
 Identities = 499/880 (56%), Positives = 619/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL       E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFALFGRLLLYNSVCPPEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTEGVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T++     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQDASTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++     +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAS-LEKGKLCSSEDDSSTSLECTGCSDDEDVA 907

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+ +H++ Y+R +  KP  A 
Sbjct: 908  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSHVHRVIYRRFVRHKPGCAQ 967

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 968  SGKHVVDST-PS--EKPITWWQYTPFFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1024

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1025 SFHLMSRRKIYAFAEGKTTDFGDIKVTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1084

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA GQ P Q              R QN+  L RK
Sbjct: 1085 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIQPEPSSHNMAGGMSGRHQNNSGLRRK 1144

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1145 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1204

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1205 WRGDRMASGTVS--VEEESGMLFSSFGLFPRPWSPLSRSSSGLEFSEVLKKFVLLGQIVA 1262

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1263 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGGALLEFQALIERKRHLESE-G 1321

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1322 KPSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1378

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL++FTE+ELERLLCGE   WNS ELLDHIKFDHGYT
Sbjct: 1379 LNSGISRQIGAFKSGFDQVFPIKHLQIFTEDELERLLCGECGFWNSNELLDHIKFDHGYT 1438

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1439 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDAD 1498

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1499 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1538


>ref|XP_009794443.1| PREDICTED: E3 ubiquitin-protein ligase UPL4 isoform X2 [Nicotiana
            sylvestris]
          Length = 1538

 Score =  939 bits (2427), Expect = 0.0
 Identities = 499/880 (56%), Positives = 619/880 (70%), Gaps = 7/880 (0%)
 Frame = -2

Query: 2673 EDNNAANHLRIMEKRFEMFGRLLLSCVDPTREEFPLVTLIRRLQSALSSVENFPIISSHT 2494
            + + + + L I+EKRF +FGRLLL       E+   + LI+RL SALSSVENFP+I SH 
Sbjct: 674  DGDGSVDQLYIVEKRFVLFGRLLLYNSVCPPEDSAFLALIKRLHSALSSVENFPVILSHA 733

Query: 2493 YKSRNSYATVPYGRCTSYPCLKVQFVRENGEVCLRDYAEDVVNVDPFVPLDEIGRYLWPR 2314
             K RNSYATVPYGRCTSYPCLKV FV+ +GE  L DY E VVNVDPF PL+ I  YLWP+
Sbjct: 734  SKLRNSYATVPYGRCTSYPCLKVHFVKGDGESSLGDYTEGVVNVDPFSPLETIEGYLWPK 793

Query: 2313 VSNDKTKNL---TLGSKDSKERDXXXXXXXXXXXXXXXXXSDVTASKEMLVDVLKQQEAK 2143
            VS  K++ L   TL  ++                       D T++     +  + QE K
Sbjct: 794  VSKRKSEKLKPPTLAIEEESSSRSSQGASTSQGKSPGPMELDTTST-----NAHETQEVK 848

Query: 2142 PNLLFSSPAGASSFAQITLDSAVVADGQTDFVEQEEHNPLKEDGGANMDHPGFSDNEDTS 1963
             NL  S         +   DS  +++     +E+ +    ++D   +++  G SD+ED +
Sbjct: 849  GNLQLSVEVETMDIEKTKSDSMDISNINAS-LEKGKLCSSEDDSSTSLECTGCSDDEDVA 907

Query: 1962 TKLLFYLEGQQLNCELTLYQSILKQQNGVERDIIPSTSVWTRIHKITYKRHMATKPNHA- 1786
             KL+FYLEGQQLN +LTLYQ++L+QQ     DII ++S+W+ +H++ Y+R +  KP  A 
Sbjct: 908  PKLIFYLEGQQLNQKLTLYQTVLQQQIKAGNDIITNSSMWSHVHRVIYRRFVRHKPGCAQ 967

Query: 1785 --KRIHDENLPSLLSKRASFFQYAPLFSPLFVSE-VDFEKSDPAYDLLSLLRSLEGINRL 1615
              K + D   PS   K  +++QY P FS +F SE VD EKS P YD+L LL+SLEG+NR 
Sbjct: 968  SGKHVVDST-PS--EKPITWWQYTPFFSSMFGSEMVDLEKSSPTYDILFLLKSLEGLNRF 1024

Query: 1614 RFHLMSHERTYGFVEGSTSDLDKLNVAVNEVPPNEFVNKKLTDKLQQQMRDPTAVSVGAM 1435
             FHLMS  + Y F EG T+D   + V  +++P NEF N KLT+KL+ QMR+P +VSVG M
Sbjct: 1025 SFHLMSRRKIYAFAEGKTTDFGDIKVTNSDLPQNEFANTKLTEKLELQMRNPFSVSVGGM 1084

Query: 1434 PAWCTQLMDWCPFLFGFEARCKYFHLAALGQSPSQXXXXXXXXXXXXXARQQNHGYLPRK 1255
            P WC QL++ CPFLFGFEARCKYF LAA GQ P Q              R QN+  L RK
Sbjct: 1085 PPWCGQLVNSCPFLFGFEARCKYFRLAAFGQPPIQPEPSSHNTAGGMSGRHQNNSGLRRK 1144

Query: 1254 KVLVHRNKILESAAQMMELHVRHKVLPEVEYNEEVGTGLGPTLEFYTLVCHEFQRSGLGM 1075
            K+LVHRN+IL+SA QMM+LH   KV+ EVEY +EVGTGLGPTLEF+TLV HEFQ+ GLGM
Sbjct: 1145 KILVHRNRILDSATQMMDLHADQKVVIEVEYTDEVGTGLGPTLEFFTLVSHEFQKIGLGM 1204

Query: 1074 WRDDCISLHCSEDLEAENSGLIVSTLGLFPRPWXXXXXXXXXXXXSEVIKKFALLGQIVA 895
            WR D ++         E SG++ S+ GLFPRPW            SEV+KKF LLGQIVA
Sbjct: 1205 WRGDRMASGTVS--VEEESGMLFSSFGLFPRPWSPLSRSSSGLEFSEVLKKFVLLGQIVA 1262

Query: 894  KSLQDGRVLDLPFSKAFYKLILGKDLTLYDIQSFDPAFGSALLEFQAIVEKRDYLRSLCG 715
            K+LQDGRVLDLP SKAFYKL+LG++LT+YDIQSFDP  G ALLEFQA++E++ +L S  G
Sbjct: 1263 KALQDGRVLDLPLSKAFYKLVLGRELTVYDIQSFDPELGGALLEFQALIERKRHLESE-G 1321

Query: 714  EKSHDLDVRLRDTKIEDLCLDFTLPGYPDYALVSGSESSDSRMVNLYNLDEYVMLMVDAT 535
            + S DL++  R+TKI+DLCLD+TLPGYPDY   S   +SD++MV++ NL+EYV L+VDA+
Sbjct: 1322 KPSLDLELNFRNTKIDDLCLDYTLPGYPDYVFNS---ASDAKMVDMSNLEEYVSLIVDAS 1378

Query: 534  TKSGIARQVEAFKSGFDQVFPIRHLKVFTEEELERLLCGEHVLWNSEELLDHIKFDHGYT 355
              SGI+RQ+ AFKSGFDQVFPI+HL++FTE+ELERLLCGE   WNS ELLDHIKFDHGYT
Sbjct: 1379 LNSGISRQIGAFKSGFDQVFPIKHLQIFTEDELERLLCGECGFWNSNELLDHIKFDHGYT 1438

Query: 354  VSSPPIVNLLEIMQEFDLDQQRAFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNAIDAD 175
             SSPP+VNLLEIM+EFD  QQRAFLQFVTGAPRLP GGLASL+PKLTIVRK CS  +DAD
Sbjct: 1439 ASSPPVVNLLEIMREFDSKQQRAFLQFVTGAPRLPPGGLASLSPKLTIVRKTCSGWVDAD 1498

Query: 174  LPSVMTCANYLKLPPYSSKEVMKEKLLYAIIEGQGSFHLS 55
            LPSVMTCANYLKLPPYSSKE MKEKLLYAI EGQGSF+LS
Sbjct: 1499 LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFYLS 1538