BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00005049
         (1688 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN22311.1| putative E3 ubiquitin ligase [Handroanthus impeti...   632   0.0  
ref|XP_011072584.1| E3 ubiquitin-protein ligase SPL2 [Sesamum in...   596   0.0  
ref|XP_012856587.1| PREDICTED: mitochondrial ubiquitin ligase ac...   553   0.0  
emb|CDO97851.1| unnamed protein product [Coffea canephora]            529   0.0  
ref|XP_017254417.1| PREDICTED: mitochondrial ubiquitin ligase ac...   523   0.0  
ref|XP_002274016.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   517   e-178
ref|XP_022889535.1| E3 ubiquitin-protein ligase SPL2 [Olea europ...   514   e-177
ref|XP_023914006.1| E3 ubiquitin-protein ligase SPL2 [Quercus su...   500   e-171
ref|XP_010086805.1| E3 ubiquitin-protein ligase SPL2 [Morus nota...   499   e-171
ref|XP_010261888.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   497   e-170
dbj|GAV82709.1| GIDE domain-containing protein/zf-C3HC4_3 domain...   497   e-170
ref|XP_019227736.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   496   e-170
ref|XP_015170730.1| PREDICTED: mitochondrial ubiquitin ligase ac...   496   e-170
ref|XP_016457579.1| PREDICTED: mitochondrial ubiquitin ligase ac...   495   e-169
ref|XP_006361337.1| PREDICTED: mitochondrial ubiquitin ligase ac...   494   e-169
ref|XP_009594761.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   494   e-169
ref|XP_021628505.1| E3 ubiquitin-protein ligase SPL2 isoform X1 ...   494   e-169
ref|XP_009781223.1| PREDICTED: mitochondrial ubiquitin ligase ac...   493   e-168
ref|XP_018835064.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   490   e-167
ref|XP_003520919.1| PREDICTED: mitochondrial ubiquitin ligase ac...   490   e-167

>gb|PIN22311.1| putative E3 ubiquitin ligase [Handroanthus impetiginosus]
          Length = 402

 Score =  632 bits (1629), Expect = 0.0
 Identities = 314/402 (78%), Positives = 337/402 (83%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MSLH+RATAAV+AQL M ADGA FGLGVAYVAY SIRKYTATSSA+RKIR APSV+    
Sbjct: 1    MSLHDRATAAVIAQLAMAADGALFGLGVAYVAYRSIRKYTATSSALRKIRDAPSVQPSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                   +FEGGKL+IVRG VE KSAAEKGN KSLRG ++VVSQ
Sbjct: 61   RSIIPDDNDPDSSHPDDGNASNSSFEGGKLLIVRGCVEAKSAAEKGNLKSLRGDSVVVSQ 120

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            E+GEKGVILQR+QTCIYNEWRGVFGWTAD+RSML+RS +EQESSSIR VPFILVE GKWP
Sbjct: 121  ETGEKGVILQRTQTCIYNEWRGVFGWTADLRSMLSRSWKEQESSSIRTVPFILVEAGKWP 180

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SDY+VVNM+GS HPLPL TVYQHLQPI ASPY FLQALFGLEYPVGLLYEEKILPLGKD
Sbjct: 181  QSDYVVVNMDGSGHPLPLTTVYQHLQPINASPYIFLQALFGLEYPVGLLYEEKILPLGKD 240

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQM+ADLSFKTKVLMW GL+F S+AIGI+
Sbjct: 241  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMLADLSFKTKVLMWSGLIFSSIAIGII 300

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GYSI RNWV+WKEWR                P EVAA+EDSGDVP+GELCVICLMRRRRS
Sbjct: 301  GYSITRNWVRWKEWRRQRRDQQQNNSTNSQTPAEVAADEDSGDVPEGELCVICLMRRRRS 360

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AFIPCGHLVCCQRCAL+VEREVSPKCPVCRQ IRSSVRIYDS
Sbjct: 361  AFIPCGHLVCCQRCALSVEREVSPKCPVCRQPIRSSVRIYDS 402


>ref|XP_011072584.1| E3 ubiquitin-protein ligase SPL2 [Sesamum indicum]
          Length = 398

 Score =  596 bits (1537), Expect = 0.0
 Identities = 306/402 (76%), Positives = 327/402 (81%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H+RA AAVLA L M ADGA FGL VAYVA  +IRKYTATSSA+R+I QAPS +    
Sbjct: 1    MSVHDRAAAAVLAHLAMAADGALFGLAVAYVACRTIRKYTATSSALRRIHQAPSAQPSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                   + EGGKL+IVRG VEVK AAEKGNWKSLRGTNLVVSQ
Sbjct: 61   RSILSEENDSDSSRLDDGSASNLDSEGGKLVIVRGSVEVKPAAEKGNWKSLRGTNLVVSQ 120

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESGEKGVILQR+QTCIYNEWRGVFGWTAD+RS+L RS +EQESSSIR VPFILVE GKWP
Sbjct: 121  ESGEKGVILQRTQTCIYNEWRGVFGWTADLRSVLGRSWKEQESSSIRTVPFILVEEGKWP 180

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SDYLVVNMEGSRHPLPL TVYQHLQPI ASPYTFLQALFGLEYPVGLLYEEKILPLGKD
Sbjct: 181  QSDYLVVNMEGSRHPLPLMTVYQHLQPINASPYTFLQALFGLEYPVGLLYEEKILPLGKD 240

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGICN+KNGI EIKSCKDLPYFL+DLTKDQ++ADLSFKTKVLMW GLVFGSLAIGIL
Sbjct: 241  ITAVGICNIKNGILEIKSCKDLPYFLTDLTKDQLVADLSFKTKVLMWSGLVFGSLAIGIL 300

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GYSIARN     + R                  EVAA+E+SGDVPDGELCV+CLMRRRRS
Sbjct: 301  GYSIARNXXXXYQQR----AQQQNNSNSNEAQAEVAADEESGDVPDGELCVVCLMRRRRS 356

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AFIPCGHLVCCQRCAL+VEREV PKCPVCRQ IRSSVRIYDS
Sbjct: 357  AFIPCGHLVCCQRCALSVEREVLPKCPVCRQPIRSSVRIYDS 398


>ref|XP_012856587.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1
            [Erythranthe guttata]
 gb|EYU21325.1| hypothetical protein MIMGU_mgv1a007519mg [Erythranthe guttata]
          Length = 404

 Score =  553 bits (1424), Expect = 0.0
 Identities = 280/406 (68%), Positives = 313/406 (77%), Gaps = 4/406 (0%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MSLH+RA  AVL QL M ADGAF G+GVAYVA+   RKY ATSSA+RKI Q+ SV+    
Sbjct: 1    MSLHDRALTAVLTQLAMAADGAFIGVGVAYVAFSCFRKYAATSSALRKISQSASVQPSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXX--NFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVV 479
                                     NF+ GKL+ V G VEVKSA +K NWKSLRGT+LVV
Sbjct: 61   RSILSDSDDSRPSSSRRDDGDASSLNFDCGKLVFVSGSVEVKSALDKANWKSLRGTDLVV 120

Query: 480  SQESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLT--RSTREQESSSIRMVPFILVEG 653
            S ESGEKGVILQR QTCIYNEWRG+F WTAD+ SML   R T+E  SSS+ MVPFILVE 
Sbjct: 121  SHESGEKGVILQRIQTCIYNEWRGLFRWTADLGSMLMPRRITKENASSSMNMVPFILVEE 180

Query: 654  GKWPPSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILP 833
            GKWPPSD++VVNMEGS HPLPL TVYQHLQP++AS YT  QAL GLEYPVGLLYEEKILP
Sbjct: 181  GKWPPSDHVVVNMEGSTHPLPLTTVYQHLQPVKASSYTVFQALVGLEYPVGLLYEEKILP 240

Query: 834  LGKDITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLA 1013
            LGKDITAVGICNLKNGIPEIKSC+DLPYFLS LTKDQMIADLSFK+KVL+W GL+ G++A
Sbjct: 241  LGKDITAVGICNLKNGIPEIKSCEDLPYFLSILTKDQMIADLSFKSKVLLWSGLILGTVA 300

Query: 1014 IGILGYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMR 1193
            IG+LGYSI+RNW KWKEW+                  +   EE+SGDVPDGELCV+CLMR
Sbjct: 301  IGVLGYSISRNWSKWKEWK--ARRAQQQQNASRIENLDETEEEESGDVPDGELCVVCLMR 358

Query: 1194 RRRSAFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            RRRSAF+PCGHLVCC RCA +VEREVSPKCPVCRQ IR+SVRIYDS
Sbjct: 359  RRRSAFVPCGHLVCCHRCAFSVEREVSPKCPVCRQPIRNSVRIYDS 404


>emb|CDO97851.1| unnamed protein product [Coffea canephora]
          Length = 396

 Score =  529 bits (1363), Expect = 0.0
 Identities = 263/404 (65%), Positives = 311/404 (76%), Gaps = 2/404 (0%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AAVL+QL + ADGA  GLG+AYVA  SI K+T+ SSA+RKI QAPS+     
Sbjct: 1    MSVHDQAAAAVLSQLALAADGAVLGLGLAYVAVRSILKFTSNSSALRKIEQAPSIRVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRG--TNLVV 479
                                     E GKL+IVRG VE KSA +  NWKSLR   + ++V
Sbjct: 61   RSVLTLPSDNDDSANVQS-------ESGKLVIVRGCVEAKSALDT-NWKSLRPAPSGVLV 112

Query: 480  SQESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGK 659
            SQESG+KGVILQR+QTCIYNEWRG FGWT+D+RS+  RS +EQESS++RMVPFILVE G+
Sbjct: 113  SQESGDKGVILQRTQTCIYNEWRGFFGWTSDLRSLFVRSWKEQESSTVRMVPFILVETGR 172

Query: 660  WPPSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLG 839
            WP SDY++VNMEGSRHPLPL TVY  LQPI ASPYTFLQALFG EYPVGLL EEK+LP G
Sbjct: 173  WPQSDYVIVNMEGSRHPLPLTTVYHRLQPIHASPYTFLQALFGHEYPVGLLDEEKLLPTG 232

Query: 840  KDITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIG 1019
            K+ITAVGIC+LK+G P IKSCKDLP+FLSDLTKDQ++ DL FKTKVL W G+V GSLA+G
Sbjct: 233  KEITAVGICSLKDGSPVIKSCKDLPFFLSDLTKDQILVDLDFKTKVLFWSGVVLGSLAVG 292

Query: 1020 ILGYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRR 1199
            ILGY++ RNW +WKEWR                  + A++E+ GDVPDGELCVICLMRRR
Sbjct: 293  ILGYAVVRNWNRWKEWRQQRRDQQQSSARSNQANDQGASDEEHGDVPDGELCVICLMRRR 352

Query: 1200 RSAFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            RSAF+PCGHLVCCQ CAL+VER++SPKCPVCRQ+IR+SVRIYDS
Sbjct: 353  RSAFVPCGHLVCCQSCALSVERDLSPKCPVCRQTIRNSVRIYDS 396


>ref|XP_017254417.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1 [Daucus
            carota subsp. sativus]
 gb|KZM89944.1| hypothetical protein DCAR_022693 [Daucus carota subsp. sativus]
          Length = 386

 Score =  523 bits (1348), Expect = 0.0
 Identities = 256/398 (64%), Positives = 305/398 (76%)
 Frame = +3

Query: 138  NRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXXXXXX 317
            ++A AAVL+Q+ + ADGA  GL +AYVA  SI KY+++SSA+RKI+ AP+V         
Sbjct: 4    DQAAAAVLSQIALAADGAVLGLTLAYVAVRSILKYSSSSSALRKIQDAPAVRVSDLRSVR 63

Query: 318  XXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQESGE 497
                                 + G++++VRG VE KS  E GNWKSLR  + +VS ESGE
Sbjct: 64   SSSDHGS--------------DEGRIIVVRGSVEAKSVVE-GNWKSLRPNHALVSPESGE 108

Query: 498  KGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWPPSDY 677
            KGV+L R+QTCIYNEWRG FGWT+DIRS+  RS +EQESSS+R VPFILV+GG WP SDY
Sbjct: 109  KGVVLLRTQTCIYNEWRGFFGWTSDIRSLFARSWKEQESSSLRTVPFILVDGGVWPYSDY 168

Query: 678  LVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKDITAV 857
            +VVNM+GSRHP+PL TVY HLQPI ASPYTFLQALFG EYPVGLL EEKILPLGKDITAV
Sbjct: 169  VVVNMDGSRHPIPLVTVYHHLQPITASPYTFLQALFGHEYPVGLLDEEKILPLGKDITAV 228

Query: 858  GICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGILGYSI 1037
            G C+LKNG PEIKSCKDLPYF+SD+TK+QM+ DL+F+TKVL WGG+V  SLAIG+L Y+ 
Sbjct: 229  GTCSLKNGTPEIKSCKDLPYFMSDMTKEQMLVDLAFRTKVLFWGGVVLSSLAIGVLSYAA 288

Query: 1038 ARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRSAFIP 1217
             RNW +WKEWR                  +V A+E++GD+PDGELCVICLM RRRSAFIP
Sbjct: 289  VRNWNRWKEWRQVRRIQQDNSAASNEPDAQVIADEETGDIPDGELCVICLMMRRRSAFIP 348

Query: 1218 CGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            CGHLVCCQRCAL++ERE+SPKCPVCRQSIR+SVRIYDS
Sbjct: 349  CGHLVCCQRCALSIEREISPKCPVCRQSIRTSVRIYDS 386


>ref|XP_002274016.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Vitis vinifera]
 emb|CBI35868.3| unnamed protein product, partial [Vitis vinifera]
          Length = 391

 Score =  517 bits (1332), Expect = e-178
 Identities = 254/402 (63%), Positives = 309/402 (76%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS H++A AA+LAQ+ + ADGA  GL +AYVA  S  + ++TSSA+R+I Q+PS      
Sbjct: 1    MSAHDQAAAAILAQVALAADGAILGLALAYVAVRSFIRLSSTSSALRQIDQSPSFRVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                       G L++VRG VE KSA + GNWKSLR  N++VS 
Sbjct: 61   RSLLSPSSDDESDQSTS--------SDGLLVVVRGTVEAKSAID-GNWKSLR-PNVLVSH 110

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESGEKGVILQR+QTCIYNEWRG FGWT+DIR++  RS +E+ES+S+R VPFILVEGG+WP
Sbjct: 111  ESGEKGVILQRTQTCIYNEWRGFFGWTSDIRTLFARSWKEKESTSLRTVPFILVEGGRWP 170

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SDY++VNM+GSRHPLPL TVY  LQP+ ASPYTFLQALFG +YPVGLL EEK+LPLGK+
Sbjct: 171  QSDYVIVNMDGSRHPLPLTTVYHQLQPVNASPYTFLQALFGHDYPVGLLDEEKLLPLGKE 230

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGIC+LKNGIPEIKSCKDLPYFLS+++KDQM+ DL FK+KVL W G++ GS++IG+L
Sbjct: 231  ITAVGICSLKNGIPEIKSCKDLPYFLSEMSKDQMVVDLVFKSKVLFWSGVILGSVSIGVL 290

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WKEWR                 T++ AE+D+GDVPDGELCVICLMRR+RS
Sbjct: 291  GYAVVRNWNRWKEWRQQRGVRPPSQAVTDDTDTQI-AEDDAGDVPDGELCVICLMRRKRS 349

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VERE+SPKCPVCRQ IRSSVRIY S
Sbjct: 350  AFVPCGHLVCCQRCALSVERELSPKCPVCRQIIRSSVRIYGS 391


>ref|XP_022889535.1| E3 ubiquitin-protein ligase SPL2 [Olea europaea var. sylvestris]
          Length = 402

 Score =  514 bits (1325), Expect = e-177
 Identities = 265/405 (65%), Positives = 315/405 (77%), Gaps = 3/405 (0%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVE-XXX 302
            MS+H++A AAVL+QL + ADGA  G+G+AYVA  SI K+TAT+SA+RKI  APSV+    
Sbjct: 1    MSVHDQAVAAVLSQLALAADGAVLGIGLAYVALRSILKFTATTSALRKIDNAPSVQPSDL 60

Query: 303  XXXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRG--TNLV 476
                                    N +GGKL+IVRG VE KSA E G    LRG  +N++
Sbjct: 61   RSLLSSCNTEGKGNTCTGGSTNQSNGDGGKLVIVRGCVEAKSAVE-GQGMILRGNTSNIL 119

Query: 477  VSQESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGG 656
            VSQES EKGVILQR+QT IYNEWRG FGWTAD+RS+  RS ++QE+S+IR VPF+LVE G
Sbjct: 120  VSQESREKGVILQRTQTYIYNEWRGFFGWTADLRSIFARSWKDQETSTIRTVPFVLVEAG 179

Query: 657  KWPPSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPL 836
            + P S Y++VNMEGS HPLPL TVY HL+PI ASPYTFLQALFG EYPVGLL  EKIL L
Sbjct: 180  R-PQSGYVIVNMEGSSHPLPLTTVYHHLKPISASPYTFLQALFGHEYPVGLLDVEKILLL 238

Query: 837  GKDITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAI 1016
            GK+ITAVGIC+L+NG PEIKSCKDLPYFLSDLTKDQ++ADL+FKTKVL+  G+VFGSLAI
Sbjct: 239  GKNITAVGICSLRNGSPEIKSCKDLPYFLSDLTKDQIMADLAFKTKVLLLSGVVFGSLAI 298

Query: 1017 GILGYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRR 1196
            GILGY+I RNW +WK+WR                P ++AA+E++GD+PDGELCVICLMRR
Sbjct: 299  GILGYAILRNWNRWKDWRQQRRAQQNNSASNDVSP-QIAADEETGDIPDGELCVICLMRR 357

Query: 1197 RRSAFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            RRSAFIPCGH+VCCQRCAL+VERE+SPKCPVCRQ+IR+SVRIYDS
Sbjct: 358  RRSAFIPCGHMVCCQRCALSVEREISPKCPVCRQTIRNSVRIYDS 402


>ref|XP_023914006.1| E3 ubiquitin-protein ligase SPL2 [Quercus suber]
          Length = 397

 Score =  500 bits (1288), Expect = e-171
 Identities = 241/402 (59%), Positives = 307/402 (76%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS   +A A++L+QL ++ DGA  G+ +AY A  S+ K++ATSS++ KI +APSV     
Sbjct: 1    MSSPEQALASLLSQLALSFDGAVLGIALAYAAVRSLLKFSATSSSLNKILKAPSV---VH 57

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                   +    KL+++RG VE KSA +  +WK+ +  N+++SQ
Sbjct: 58   VSDLRSILAVHDDQDSSSSSQPSDHHHQKLVVLRGTVEAKSAVDS-SWKTFK-PNVLLSQ 115

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESG+K V++QR+QTCIYNEW+G FGW +D+R++  RS REQES+SIR VPF+LV+GG+WP
Sbjct: 116  ESGDKAVVVQRTQTCIYNEWKGFFGWPSDLRAIFARSRREQESTSIRAVPFVLVDGGRWP 175

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SD++VVNM+GS HPLPL TV+  LQPI ASPYTFLQALFG EYPVGLL EEKILPLGKD
Sbjct: 176  HSDFVVVNMDGSNHPLPLTTVFHQLQPINASPYTFLQALFGHEYPVGLLDEEKILPLGKD 235

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ KNG+PEIKSCKDLPYFLSDLTKDQMI DL+F++K+L+WGG+ FGSL+IGIL
Sbjct: 236  ITAVGMCSFKNGVPEIKSCKDLPYFLSDLTKDQMIVDLAFRSKILLWGGVAFGSLSIGIL 295

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WKEWR                 T++A+EE++GDVPDGELCVICLMRRRRS
Sbjct: 296  GYAVMRNWNRWKEWRQNRQLQRQSQAASPDADTQIASEEETGDVPDGELCVICLMRRRRS 355

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AFIPCGHLVCCQ CA++VERE +PKCPVCRQ IR+S+RIYD+
Sbjct: 356  AFIPCGHLVCCQLCAISVERESTPKCPVCRQEIRNSLRIYDT 397


>ref|XP_010086805.1| E3 ubiquitin-protein ligase SPL2 [Morus notabilis]
 gb|EXB23815.1| Mitochondrial ubiquitin ligase activator of nfkb 1 [Morus notabilis]
          Length = 393

 Score =  499 bits (1285), Expect = e-171
 Identities = 245/402 (60%), Positives = 303/402 (75%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS + +A  ++L+QL ++ DGA  G+ +AY A  S+ KYT+TSSA+ K+R+APSV+    
Sbjct: 1    MSSNEQAVVSLLSQLALSFDGAVLGVALAYAAVRSVLKYTSTSSALCKLRKAPSVQVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                       GKL+IVRG VE KSA + G+WKSLR  N++VSQ
Sbjct: 61   RSILAADASSEGSNESRP-------SDGKLVIVRGTVEAKSAID-GSWKSLR-PNVLVSQ 111

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESG+K V++QR+QTCIYNEW+G FGWT+D+R++  RS REQES+S+R V FILVE GKWP
Sbjct: 112  ESGDKAVVIQRTQTCIYNEWKGFFGWTSDLRTIFARSWREQESTSLRTVSFILVEAGKWP 171

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              D++VVNM+GSRHPLPL TVY  LQP+  SPYTFLQALFG EYPVGLL EEKILPLGKD
Sbjct: 172  QMDFVVVNMDGSRHPLPLTTVYHQLQPVNPSPYTFLQALFGHEYPVGLLDEEKILPLGKD 231

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGIC+ KNGI EIKSCKD+PYFL+++TKDQM+ DL+F TKVL+  G+V GSL+IGIL
Sbjct: 232  ITAVGICSFKNGIAEIKSCKDIPYFLTEMTKDQMVVDLAFSTKVLLVSGIVLGSLSIGIL 291

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WKEWRH                +++ +EED+ DVPDG+LCVICL RRRRS
Sbjct: 292  GYALVRNWNRWKEWRHNRQQQRANPPAIDDSESQIVSEEDTTDVPDGQLCVICLTRRRRS 351

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AFIPCGHLVCC  CA++VEREV+PKCPVCRQ IR+SVRIYDS
Sbjct: 352  AFIPCGHLVCCHICAISVEREVTPKCPVCRQEIRTSVRIYDS 393


>ref|XP_010261888.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Nelumbo nucifera]
          Length = 391

 Score =  497 bits (1279), Expect = e-170
 Identities = 242/402 (60%), Positives = 303/402 (75%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS H+RA AA LA + + ADGA  GL +AYVA  S  K+  TSSA++KIR AP+V     
Sbjct: 1    MSSHDRAAAAALANVALAADGAVIGLVLAYVAVQSWLKFNNTSSALQKIRGAPTVRVADL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                       GKL+IVRG++E KSA + GNW+SLR  N ++S 
Sbjct: 61   RSLLSSSDDNSIRNIANESGPTE----GKLVIVRGLIEAKSAVD-GNWRSLR-PNALISH 114

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESGE+ V+LQR+QTCIYNEWRG+FGWT+D+R++  R  +EQ+S+S+RMVPFILVEGG+WP
Sbjct: 115  ESGERAVVLQRTQTCIYNEWRGLFGWTSDLRTLFARPLKEQKSTSLRMVPFILVEGGQWP 174

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SDY+VVN++GSRHPLPL TVY  LQP++ASPYTFLQALFG  YPVGLL EEKILPLGK+
Sbjct: 175  HSDYVVVNLDGSRHPLPLVTVYHQLQPVQASPYTFLQALFGHHYPVGLLDEEKILPLGKE 234

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGIC  ++G PEIKSCKDLPYFLSD+TKDQ++ DL F+TKVL+WGG++  SL+ GIL
Sbjct: 235  ITAVGICRFQDGNPEIKSCKDLPYFLSDMTKDQIVVDLGFRTKVLLWGGILLVSLSFGIL 294

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY+I RNW +WKEW+                  E+  +E++GDVPDG+LCVICLMRRRRS
Sbjct: 295  GYAIMRNWNRWKEWKQ-----QRQVQQSRAAAPEIEGDEEAGDVPDGQLCVICLMRRRRS 349

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQ CAL+VER+++PKCPVCRQ+IR+S+R+Y S
Sbjct: 350  AFVPCGHLVCCQGCALSVERDLAPKCPVCRQTIRNSIRVYSS 391


>dbj|GAV82709.1| GIDE domain-containing protein/zf-C3HC4_3 domain-containing protein
            [Cephalotus follicularis]
          Length = 397

 Score =  497 bits (1279), Expect = e-170
 Identities = 241/403 (59%), Positives = 307/403 (76%), Gaps = 2/403 (0%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            M  H +A A++++QL ++ DGA  G+ +AY A  S+ K+ A+SSA+RKIR+AP +     
Sbjct: 1    MPTHEQALASLVSQLALSFDGAVLGVALAYAAVRSLLKFRASSSALRKIRRAPQLSVPDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     +  +++IV GIV+ KSA +  NWKS R  +++V+Q
Sbjct: 61   RALLNYNQSQTSHPNHHEQQ-----QQEEIVIVHGIVDAKSAVDS-NWKSFR-PHVLVAQ 113

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESG+K VILQR+QTCIYNEW+G FGW++D+R++  RS REQES+S+R VPFILVEGG+WP
Sbjct: 114  ESGDKAVILQRTQTCIYNEWKGFFGWSSDLRAIFGRSLREQESTSLRTVPFILVEGGQWP 173

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             SDY+VVNM+GSRHPLPL TVY  LQPI+ASPYTFLQALFG EYPVGLL EEKILPLGK+
Sbjct: 174  QSDYVVVNMDGSRHPLPLTTVYHQLQPIDASPYTFLQALFGHEYPVGLLDEEKILPLGKE 233

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            I AVG C+++NGIPEIKSCKDLPYFL D+TKDQ++ DL+FKTK+L+WGG+VFGSL+IGIL
Sbjct: 234  INAVGFCSIRNGIPEIKSCKDLPYFLFDVTKDQLVVDLAFKTKILLWGGIVFGSLSIGIL 293

Query: 1026 GYSIARNWVKWKEW--RHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRR 1199
            GY++ RNW +WK+W  R                 +++ A+E++GDV DGELCVICLMRRR
Sbjct: 294  GYAVVRNWNRWKDWRQRRQLQQPSHSTNDEVNSQSQIPADEEAGDVADGELCVICLMRRR 353

Query: 1200 RSAFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYD 1328
             SAFIPCGHLVCCQRCAL++ERE SPKCPVCRQ++RSS+RIYD
Sbjct: 354  LSAFIPCGHLVCCQRCALSIEREGSPKCPVCRQTVRSSIRIYD 396


>ref|XP_019227736.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Nicotiana attenuata]
 gb|OIT31193.1| e3 ubiquitin-protein ligase spl2 [Nicotiana attenuata]
          Length = 391

 Score =  496 bits (1277), Expect = e-170
 Identities = 245/402 (60%), Positives = 301/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AAV  +L M ADGA  GL +AY+A  SI  + A SSA+ KI  APS+     
Sbjct: 1    MSVHDQAAAAV--KLAMAADGALVGLALAYIAVRSIFMFKANSSALHKINDAPSLRVSDL 58

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     +GGKL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 59   RSVISSSSESNVNSDQSD-------DGGKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 109

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ES E+GVILQR+QTCIYNEWRG FGWT D+RS+  R  +EQ+SSS+RMVPF++VE G+WP
Sbjct: 110  ESAEQGVILQRTQTCIYNEWRGFFGWTGDLRSIFPRYWKEQDSSSLRMVPFVVVEAGRWP 169

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+GSRHPLPL TVY HLQP+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 170  QPEYVNVNMDGSRHPLPLVTVYHHLQPVNATPLTFLQALFGHPYPVGVLDEEKILPLGKD 229

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ K+GI EIKSCKDLPYFLSD+TKDQM+ DL+FKTKVL+W G+V GS A+G+L
Sbjct: 230  ITAVGVCSSKDGIFEIKSCKDLPYFLSDMTKDQMLVDLAFKTKVLLWSGVVLGSFAVGVL 289

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY+  RNW +WKE RH                ++V+A+E++GDVPDG+LCVICLMRRRRS
Sbjct: 290  GYAAVRNWNRWKERRHQRQVQQQNASASNEADSQVSADEETGDVPDGQLCVICLMRRRRS 349

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCPVCRQ+IR SV IYDS
Sbjct: 350  AFVPCGHLVCCQRCALSVERDLAPKCPVCRQTIRCSVMIYDS 391


>ref|XP_015170730.1| PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1-like
            isoform X2 [Solanum tuberosum]
          Length = 393

 Score =  496 bits (1277), Expect = e-170
 Identities = 241/402 (59%), Positives = 299/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AAVL+Q+ M ADGA  GL +AYVA  SI K+ A SSA+ KI  AP +     
Sbjct: 1    MSIHDQAAAAVLSQIAMAADGALIGLALAYVAVRSILKFKANSSALHKINDAPLL----- 55

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     +  KL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 56   --GVSDLRSLLSSSDSNNTESSNQSDDVKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 111

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
             S EKGVILQ +QTCIYNEWRG F W  D+ S+  R  ++Q+SSS+RMVPF+LVE GKWP
Sbjct: 112  NSAEKGVILQHTQTCIYNEWRGFFSWAGDLYSIFPRVRKDQQSSSLRMVPFVLVETGKWP 171

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+GSRHPLPL TVY HL+P+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 172  QPEYVNVNMDGSRHPLPLVTVYHHLRPLHATPLTFLQALFGHHYPVGVLDEEKILPLGKD 231

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ K+GI EIKSC+DLPYFLSD+TKDQM+ DL+FKTKVLMW G+VFGS+A+G+L
Sbjct: 232  ITAVGVCSSKDGILEIKSCEDLPYFLSDMTKDQMLVDLAFKTKVLMWSGVVFGSVAVGVL 291

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WK+WRH                 +V A+E++GDVPDG+LCVICLMRR+RS
Sbjct: 292  GYAVVRNWNRWKQWRHQRQAQQQRGSASNDDDLQVTADEETGDVPDGQLCVICLMRRKRS 351

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCP+CRQ+I SSVRIYDS
Sbjct: 352  AFVPCGHLVCCQRCALSVERDLAPKCPLCRQTIHSSVRIYDS 393


>ref|XP_016457579.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            [Nicotiana tabacum]
          Length = 392

 Score =  495 bits (1274), Expect = e-169
 Identities = 245/402 (60%), Positives = 300/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AA + +L M ADGA  GL +AY+A  SI  + A SSA+ KI  APS+     
Sbjct: 1    MSVHDQAAAAAV-KLAMAADGALVGLALAYIAVRSIFMFKAKSSALHKINDAPSLRVSDL 59

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     + GKL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 60   RSLISLSSESNVNSDQSD-------DDGKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 110

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ES EKGVILQR+QTCIYNEWRG FGWT D+RS+  R  +EQ+SSS+RMVPF++VE G+WP
Sbjct: 111  ESAEKGVILQRTQTCIYNEWRGFFGWTGDLRSIFPRYWKEQDSSSLRMVPFVVVEAGRWP 170

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+GSRHPLPL TVY HLQP+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 171  QPEYVNVNMDGSRHPLPLVTVYHHLQPVNATPLTFLQALFGHPYPVGVLDEEKILPLGKD 230

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ K+GI EIKSCKDLPYFLSD+TKDQM+ DL+FKTKVL+W G+V GS A+G+L
Sbjct: 231  ITAVGVCSSKDGIFEIKSCKDLPYFLSDMTKDQMLVDLAFKTKVLLWSGVVLGSFAVGVL 290

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY+  RNW +WKE RH                ++V+A+E++GDVPDG+LCVICLMRRRRS
Sbjct: 291  GYAAVRNWNRWKERRHHRQVQQQNASASNEADSQVSADEETGDVPDGQLCVICLMRRRRS 350

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCPVCRQSI SSV IYDS
Sbjct: 351  AFVPCGHLVCCQRCALSVERDLAPKCPVCRQSIHSSVMIYDS 392


>ref|XP_006361337.1| PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1-like
            isoform X1 [Solanum tuberosum]
          Length = 393

 Score =  494 bits (1273), Expect = e-169
 Identities = 240/402 (59%), Positives = 298/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AAVL+Q+ M ADGA  GL +AYVA  SI K+ A SSA+ KI  AP +     
Sbjct: 1    MSIHDQAAAAVLSQIAMAADGALIGLALAYVAVRSILKFKANSSALHKINDAPLL----- 55

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     +  KL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 56   --GVSDLRSLLSSSDSNNTESSNQSDDVKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 111

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
             S EKGVILQ +QTCIYNEWRG F W  D+ S+  R  ++Q+SSS+RMVPF+ VE GKWP
Sbjct: 112  NSAEKGVILQHTQTCIYNEWRGFFSWAGDLYSIFPRVRKDQQSSSLRMVPFVFVETGKWP 171

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+GSRHPLPL TVY HL+P+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 172  QPEYVNVNMDGSRHPLPLVTVYHHLRPLHATPLTFLQALFGHHYPVGVLDEEKILPLGKD 231

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ K+GI EIKSC+DLPYFLSD+TKDQM+ DL+FKTKVLMW G+VFGS+A+G+L
Sbjct: 232  ITAVGVCSSKDGILEIKSCEDLPYFLSDMTKDQMLVDLAFKTKVLMWSGVVFGSVAVGVL 291

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WK+WRH                 +V A+E++GDVPDG+LCVICLMRR+RS
Sbjct: 292  GYAVVRNWNRWKQWRHQRQAQQQRGSASNDDDLQVTADEETGDVPDGQLCVICLMRRKRS 351

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCP+CRQ+I SSVRIYDS
Sbjct: 352  AFVPCGHLVCCQRCALSVERDLAPKCPLCRQTIHSSVRIYDS 393


>ref|XP_009594761.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Nicotiana
            tomentosiformis]
          Length = 392

 Score =  494 bits (1272), Expect = e-169
 Identities = 245/402 (60%), Positives = 300/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AA + +L M ADGA  GL +AY+A  SI  + A SSA+ KI  APS+     
Sbjct: 1    MSVHDQAAAAAV-KLAMAADGALVGLALAYIAVRSIFMFKAKSSALHKINDAPSLRVSDL 59

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     + GKL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 60   RSLISLSSESNVNSDQSD-------DDGKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 110

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ES EKGVILQR+QTCIYNEWRG FGWT D+RS+  R  +EQ+SSS+RMVPF++VE G+WP
Sbjct: 111  ESAEKGVILQRTQTCIYNEWRGFFGWTGDLRSIFPRYWKEQDSSSLRMVPFVVVEAGRWP 170

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+GSRHPLPL TVY HLQP+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 171  QPEYVNVNMDGSRHPLPLVTVYHHLQPVNATPLTFLQALFGHPYPVGVLDEEKILPLGKD 230

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVGIC+ K+GI EIKSCKDLPYFLSD+TKDQM+ DL++KTKVL+W G+V GS A+G+L
Sbjct: 231  ITAVGICSSKDGIFEIKSCKDLPYFLSDMTKDQMLVDLAYKTKVLLWSGVVLGSFAVGVL 290

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY+  RNW +WKE RH                ++V+A+E++GDVPDG+LCVICLMRRRRS
Sbjct: 291  GYAAVRNWNRWKERRHQRQVQQQNASASNEADSQVSADEETGDVPDGQLCVICLMRRRRS 350

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCPVCRQSI SSV IYDS
Sbjct: 351  AFVPCGHLVCCQRCALSVERDLAPKCPVCRQSIHSSVMIYDS 392


>ref|XP_021628505.1| E3 ubiquitin-protein ligase SPL2 isoform X1 [Manihot esculenta]
 gb|OAY36412.1| hypothetical protein MANES_11G019800 [Manihot esculenta]
          Length = 396

 Score =  494 bits (1272), Expect = e-169
 Identities = 242/404 (59%), Positives = 305/404 (75%), Gaps = 3/404 (0%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS H +  A++++QL ++ DGA  G+ +AY A  S  K+T+ S A+RKI +AP++     
Sbjct: 1    MSSHEQVLASLVSQLALSFDGAILGVALAYAAVRSFLKFTSNSKALRKISKAPTLRVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGG---KLMIVRGIVEVKSAAEKGNWKSLRGTNLV 476
                                   N + G   KL+IVRG VE KSA + GNWKSLR +N +
Sbjct: 61   RSVLDLPQPSDENQNQ-------NVDQGQTQKLVIVRGQVEPKSAVD-GNWKSLR-SNAL 111

Query: 477  VSQESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGG 656
            VS E G+K VI+QR+QTCIYNEW+G FGWT+DIR++  RS REQES+S+RMVPF+LVEGG
Sbjct: 112  VSHECGDKAVIIQRTQTCIYNEWKGFFGWTSDIRAIFGRSWREQESTSLRMVPFVLVEGG 171

Query: 657  KWPPSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPL 836
            +WP SDY++VN++GSRH LPL TVY  LQPI+ASPYTFLQALFG EYPVGLL EEKILPL
Sbjct: 172  RWPQSDYVIVNIDGSRHALPLTTVYHQLQPIDASPYTFLQALFGHEYPVGLLDEEKILPL 231

Query: 837  GKDITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAI 1016
            GK+I AVGIC+ +NG+ EIKSCKDLPYFLSD+TKDQM+ DL+FKTKVL+W G+V GSL+I
Sbjct: 232  GKEINAVGICSSRNGVLEIKSCKDLPYFLSDMTKDQMVVDLAFKTKVLLWSGVVLGSLSI 291

Query: 1017 GILGYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRR 1196
            GILGY+  RNW +WKEWR                  ++ A+E++GDVPDG+LCVICLMRR
Sbjct: 292  GILGYAAVRNWNRWKEWRQHRQSQQQNHAASTDDDPQIDADEEAGDVPDGQLCVICLMRR 351

Query: 1197 RRSAFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYD 1328
            RRSAFIPCGHLVCCQ CA++VEREVSPKCP+CRQ++R+S+RI++
Sbjct: 352  RRSAFIPCGHLVCCQLCAISVEREVSPKCPLCRQAVRNSMRIFE 395


>ref|XP_009781223.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1
            [Nicotiana sylvestris]
 ref|XP_016455630.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            [Nicotiana tabacum]
          Length = 391

 Score =  493 bits (1268), Expect = e-168
 Identities = 243/402 (60%), Positives = 299/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS+H++A AAV  +L M ADGA  GL +AY+A  SI  + A SSA+ KI  APS+     
Sbjct: 1    MSVHDQAAAAV--KLAMAADGALVGLALAYIAVRSIFIFKANSSALHKINDAPSLRVSDL 58

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                     +GGKL+I+RG VE KSA E GNWKSLR  N++ + 
Sbjct: 59   RSVISSSSESNVNSDQSD-------DGGKLVIIRGTVEAKSAVE-GNWKSLRA-NILSAH 109

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ES EKGVILQR+QTCIYNEWRG FGWT D+RS+  R  +EQ+SSS+RMVPF++VE G+WP
Sbjct: 110  ESAEKGVILQRTQTCIYNEWRGFFGWTGDLRSIFPRYWKEQDSSSLRMVPFVIVEAGRWP 169

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
              +Y+ VNM+ SRHPLPL TVY HLQP+ A+P TFLQALFG  YPVG+L EEKILPLGKD
Sbjct: 170  QPEYVNVNMDSSRHPLPLVTVYHHLQPVNATPLTFLQALFGHPYPVGVLDEEKILPLGKD 229

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+ K+GI EIKSCKDLPYFLSD+TKDQM+ DL+FKTKVL+W G+V G  A+G+L
Sbjct: 230  ITAVGVCSSKDGILEIKSCKDLPYFLSDMTKDQMLVDLAFKTKVLLWSGVVLGLFAVGVL 289

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY+  RNW +WKE RH                ++++A+E++GDVPDG+LCVICLMRRRRS
Sbjct: 290  GYAAVRNWNRWKERRHQRQVQQQNASASNEADSQISADEETGDVPDGQLCVICLMRRRRS 349

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF+PCGHLVCCQRCAL+VER+++PKCPVCRQ+IR SV IYDS
Sbjct: 350  AFVPCGHLVCCQRCALSVERDLAPKCPVCRQTIRCSVMIYDS 391


>ref|XP_018835064.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Juglans regia]
          Length = 391

 Score =  490 bits (1262), Expect = e-167
 Identities = 240/402 (59%), Positives = 301/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS H +A  ++L+QL ++ DGA  G+ +AY A  S+ K+TATSS++RKIR+APS++    
Sbjct: 1    MSSHEQALVSLLSQLALSLDGAVLGVALAYAAVRSLLKFTATSSSLRKIRKAPSLKVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                         +++VRG VE KSA +  NWK+ +  N++VSQ
Sbjct: 61   RSILTVDDDSSEGQSHPSDQ--------SIVVVRGTVEAKSAVDP-NWKNFK-PNVLVSQ 110

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESG+K V++QR+QTCIYNEW+GVFGWT+D+R++  RS REQES+S+R VPF+LVEG  W 
Sbjct: 111  ESGDKAVVIQRTQTCIYNEWKGVFGWTSDLRALFARSWREQESTSLRTVPFVLVEGYGWS 170

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
            P D++VVN++GSRH LPL TVY  LQPI ASPYTFLQALFG EYPVG+L E KILPLGKD
Sbjct: 171  P-DFVVVNVDGSRHCLPLTTVYHQLQPINASPYTFLQALFGHEYPVGMLDEVKILPLGKD 229

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C++KNGIPEIKSCKDLPYFLSD+TKDQMI DL+F++KVL+W G    SL+IGIL
Sbjct: 230  ITAVGLCSVKNGIPEIKSCKDLPYFLSDMTKDQMIVDLAFRSKVLLWSGFALSSLSIGIL 289

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW +WKEWR                 +++AAEE++GDVPDGELCVICLMRRRRS
Sbjct: 290  GYAVVRNWSRWKEWRQHRQLQQHRQATSNDAASQIAAEEETGDVPDGELCVICLMRRRRS 349

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
            AF PCGHLVCCQ CA++VERE +PKCPVCRQ IR S+RIYDS
Sbjct: 350  AFDPCGHLVCCQSCAISVERESTPKCPVCRQEIRKSLRIYDS 391


>ref|XP_003520919.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            isoform X2 [Glycine max]
 gb|KRH66057.1| hypothetical protein GLYMA_03G080300 [Glycine max]
          Length = 388

 Score =  490 bits (1261), Expect = e-167
 Identities = 237/402 (58%), Positives = 301/402 (74%)
 Frame = +3

Query: 126  MSLHNRATAAVLAQLMMTADGAFFGLGVAYVAYCSIRKYTATSSAVRKIRQAPSVEXXXX 305
            MS   +A  ++L+QL ++ DGA  GL +AY A  ++ K+TATS+A+RK+ +APSV     
Sbjct: 1    MSHQEQAFVSLLSQLALSFDGAVLGLALAYAAVRTLFKFTATSAALRKLHRAPSVSVSDL 60

Query: 306  XXXXXXXXXXXXXXXXXXXXXXXNFEGGKLMIVRGIVEVKSAAEKGNWKSLRGTNLVVSQ 485
                                   N +GG ++IVRG V+ KS  E G WK+LR   ++VS+
Sbjct: 61   RSLLAEIPSDADG----------NSDGGNIVIVRGTVDAKSTVEGGTWKTLR-PGVLVSR 109

Query: 486  ESGEKGVILQRSQTCIYNEWRGVFGWTADIRSMLTRSTREQESSSIRMVPFILVEGGKWP 665
            ESG+K VILQR+QTCIYNEW+G+FGWT+D+R++  RS R+QES+S+R VPF+L++ G+WP
Sbjct: 110  ESGDKSVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQESTSLRKVPFVLIDVGRWP 169

Query: 666  PSDYLVVNMEGSRHPLPLATVYQHLQPIEASPYTFLQALFGLEYPVGLLYEEKILPLGKD 845
             ++Y+VVNM+GSRHPLPL+TVY  LQPI ASPYTFLQALFG EYPVGLL EEKILPLGK+
Sbjct: 170  NAEYVVVNMDGSRHPLPLSTVYHKLQPITASPYTFLQALFGHEYPVGLLDEEKILPLGKN 229

Query: 846  ITAVGICNLKNGIPEIKSCKDLPYFLSDLTKDQMIADLSFKTKVLMWGGLVFGSLAIGIL 1025
            ITAVG+C+LKNGI EIKSCKDLPYFLSDL+KDQMI DLS KTK+L WGG+  GS+++G+L
Sbjct: 230  ITAVGLCSLKNGIAEIKSCKDLPYFLSDLSKDQMIVDLSSKTKILFWGGIALGSMSVGVL 289

Query: 1026 GYSIARNWVKWKEWRHXXXXXXXXXXXXXXXPTEVAAEEDSGDVPDGELCVICLMRRRRS 1205
            GY++ RNW KWK+W+                P     +++  DVPDG+LCVICLMRRRRS
Sbjct: 290  GYAVVRNWNKWKQWKQQRQLQQQRQAVSDVEP---QMDDEIEDVPDGQLCVICLMRRRRS 346

Query: 1206 AFIPCGHLVCCQRCALAVEREVSPKCPVCRQSIRSSVRIYDS 1331
             FIPCGHLVCCQ CA++VEREV+PKCPVCRQ IR SVRIY+S
Sbjct: 347  VFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVRIYES 388