BLASTX nr result
ID: Rehmannia30_contig00019368
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia30_contig00019368
(1087 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|PIN26934.1| hypothetical protein CDL12_00291 [Handroanthus im... 240 8e-71
gb|PIN17098.1| hypothetical protein CDL12_10239 [Handroanthus im... 232 1e-67
ref|XP_011072452.1| protein CHUP1, chloroplastic [Sesamum indicum] 227 3e-65
gb|PIN24201.1| hypothetical protein CDL12_03071 [Handroanthus im... 209 3e-58
ref|XP_011080368.1| protein CHUP1, chloroplastic [Sesamum indicum] 199 1e-54
ref|XP_022885085.1| protein CHUP1, chloroplastic isoform X2 [Ole... 198 3e-54
ref|XP_022885084.1| protein CHUP1, chloroplastic isoform X1 [Ole... 198 3e-54
gb|EYU45839.1| hypothetical protein MIMGU_mgv1a018903mg, partial... 170 3e-45
ref|XP_012856299.1| PREDICTED: protein CHUP1, chloroplastic [Ery... 170 3e-44
gb|KZV58819.1| hypothetical protein F511_18656 [Dorcoceras hygro... 170 6e-44
ref|XP_009595893.1| PREDICTED: protein CHUP1, chloroplastic-like... 165 4e-42
emb|CDO97961.1| unnamed protein product [Coffea canephora] 164 1e-41
ref|XP_016483970.1| PREDICTED: protein CHUP1, chloroplastic-like... 161 1e-40
ref|XP_009790396.1| PREDICTED: protein CHUP1, chloroplastic-like... 161 1e-40
ref|XP_019262213.1| PREDICTED: protein CHUP1, chloroplastic-like... 160 2e-40
ref|XP_019243552.1| PREDICTED: protein CHUP1, chloroplastic-like... 160 3e-40
ref|XP_009590753.1| PREDICTED: protein CHUP1, chloroplastic-like... 157 3e-39
ref|XP_012836445.1| PREDICTED: protein CHUP1, chloroplastic-like... 152 3e-38
ref|XP_009790609.1| PREDICTED: protein CHUP1, chloroplastic-like... 152 2e-37
gb|EYU21441.1| hypothetical protein MIMGU_mgv1a004777mg [Erythra... 149 9e-37
>gb|PIN26934.1| hypothetical protein CDL12_00291 [Handroanthus impetiginosus]
Length = 566
Score = 240 bits (613), Expect = 8e-71
Identities = 147/303 (48%), Positives = 178/303 (58%), Gaps = 20/303 (6%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGEL 673
M VKEKR++P+ PVLFKFGV LAFSLGGIVY+ FR+ E+
Sbjct: 1 MVVKEKRESPVGPVLFKFGVALAFSLGGIVYSLFRSKIIKPSKSKPSLGSSGMASEADSR 60
Query: 672 GDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC----------- 526
GDD RA Q++S+++V S RCS DRD FL +E D L+ EC
Sbjct: 61 GDD-RASQEISLVKVSSEDSINDLSSSGRCSGDRDGFLVAEFDQLVKECDMAFEQSLVPA 119
Query: 525 ---------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYGLKEQETAVMELQNRL 373
AEHE+++ EI+SL++KV EYYGLKEQETAVMELQNRL
Sbjct: 120 VDLPLECKIAEHEKQEREIRSLQSKVKVLEERERMLEIQLLEYYGLKEQETAVMELQNRL 179
Query: 372 RIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXXXXXXXLMSEAEQNR 193
R++NME KLY+LKIESLQS+NRRL+AQVADY +VV ELE L SEAEQNR
Sbjct: 180 RVNNMETKLYSLKIESLQSENRRLQAQVADYAEVVAELEAAKAKIKLLRKKLKSEAEQNR 239
Query: 192 EQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXXXXXXXXXXENSELAQ 13
EQ+L L+ERVMKLQ+EE G EIDRD++MQ ENSELA+
Sbjct: 240 EQILKLKERVMKLQEEENKGGEIDRDLEMQLQKMKELEEELEEVKKSNHCLKQENSELAE 299
Query: 12 KLE 4
KLE
Sbjct: 300 KLE 302
>gb|PIN17098.1| hypothetical protein CDL12_10239 [Handroanthus impetiginosus]
Length = 566
Score = 232 bits (592), Expect = 1e-67
Identities = 142/303 (46%), Positives = 176/303 (58%), Gaps = 20/303 (6%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGEL 673
M +KEKR++P+ PVLFKFGV LAFSLGGIVY+ FR+ E+
Sbjct: 1 MGLKEKRESPVGPVLFKFGVALAFSLGGIVYSLFRSKIIKPSKSKPSLGSSGMASEADSR 60
Query: 672 GDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC----------- 526
GDD A Q+LS ++V S RCS DRD FL +E+D L+ EC
Sbjct: 61 GDDL-ASQELSPVKVSSEDSINDLSSSGRCSGDRDGFLVAELDQLVKECDMAFEQSLVPA 119
Query: 525 ---------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYGLKEQETAVMELQNRL 373
AEHEE++ EI++L++KV EYYGLKEQETA+MELQNRL
Sbjct: 120 VDLPLECKIAEHEEQEREIRNLQSKVKILEERERMLEIQLLEYYGLKEQETAIMELQNRL 179
Query: 372 RIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXXXXXXXLMSEAEQNR 193
R++NME KLY+LKIESLQS+NRR++AQVADY +VV ELE L SEAEQNR
Sbjct: 180 RVNNMETKLYSLKIESLQSENRRIQAQVADYAEVVAELEAAKAKIKLLRKKLKSEAEQNR 239
Query: 192 EQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXXXXXXXXXXENSELAQ 13
EQ+L L+ERVMKLQ+EE G EI +D++MQ ENSELA+
Sbjct: 240 EQILKLKERVMKLQEEENKGGEIGQDLEMQLQKMKELEEELEEVKKSNHSLKQENSELAE 299
Query: 12 KLE 4
KLE
Sbjct: 300 KLE 302
>ref|XP_011072452.1| protein CHUP1, chloroplastic [Sesamum indicum]
Length = 630
Score = 227 bits (579), Expect = 3e-65
Identities = 148/318 (46%), Positives = 173/318 (54%), Gaps = 35/318 (11%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDN------QVDSR 691
M VKEKR++PI+PVL K G LAFSLGGIVYTFFR+ N QV SR
Sbjct: 1 MGVKEKRESPISPVLLKLGAALAFSLGGIVYTFFRSKRIKPSVPKPALNSPGKGSQVCSR 60
Query: 690 GESGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC----- 526
E G + D RALQD S+ ++ S RC+ DRD FL E L+ EC
Sbjct: 61 REDGGVAD--RALQDSSLPKIASQNSISDLSSSGRCNGDRDGFLLPEFSQLVKECNMVPT 118
Query: 525 ------------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYG 418
AE EE+ +I+SLRNKV EYYG
Sbjct: 119 DDISPGMSGQSLAPDVASPLGNKYAEDEEQTRDIRSLRNKVKILEERERYLEVQLLEYYG 178
Query: 417 LKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXX 238
LKEQ+ A+MELQNRLR++NME KLYNLKIESLQ+DNRRL+AQVADY KVV ELE
Sbjct: 179 LKEQQNALMELQNRLRLNNMETKLYNLKIESLQADNRRLQAQVADYAKVVAELEAAKAKI 238
Query: 237 XXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXX 58
L S+AEQNRE +L LQERVMKL+D E VE+D+DV+MQ
Sbjct: 239 KLLRKKLRSDAEQNREHILSLQERVMKLRDPEMKAVEVDQDVEMQLKKRKELEEELEEMK 298
Query: 57 XXXXXXXXENSELAQKLE 4
ENSELAQKLE
Sbjct: 299 RSNDSLKLENSELAQKLE 316
>gb|PIN24201.1| hypothetical protein CDL12_03071 [Handroanthus impetiginosus]
Length = 650
Score = 209 bits (532), Expect = 3e-58
Identities = 139/313 (44%), Positives = 165/313 (52%), Gaps = 30/313 (9%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGEL 673
MAVK+KRD PI PV+ KFGV +A SLGG++YTF N ESGEL
Sbjct: 52 MAVKQKRDIPIGPVVLKFGVAIAISLGGVLYTFLSGKRMKPPKSKPSGN------ESGEL 105
Query: 672 GDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINECA---------- 523
DD ALQD S ++ S R S DR+ L E + L+ EC
Sbjct: 106 RDDDGALQDSSFRKISSQNSFSGRSSSVRHSGDREGLLLPEFNDLVKECNISVTIDDISP 165
Query: 522 --------------------EHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYGLKEQE 403
E +E + EIK L+ V EYYG+KEQE
Sbjct: 166 RKTIESLVSDVESPKEYKCNEPDEHEREIKRLQTMVKFLEERERNLEIQLFEYYGIKEQE 225
Query: 402 TAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXXXXXX 223
TAVMELQNRLR++NMEAKLYNLKIESL SDNRRLE QVADY KVVTELE
Sbjct: 226 TAVMELQNRLRLNNMEAKLYNLKIESLLSDNRRLEEQVADYAKVVTELEAAKAKIKLLRK 285
Query: 222 XLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXXXXXXX 43
L EAEQNREQ+L LQ+RVMKLQD+E+ VE+++D++MQ
Sbjct: 286 KLKLEAEQNREQILNLQDRVMKLQDQEQKSVEMNQDLEMQLKIKKELEEELEEMKKLNQS 345
Query: 42 XXXENSELAQKLE 4
ENS+LAQKLE
Sbjct: 346 LKFENSDLAQKLE 358
>ref|XP_011080368.1| protein CHUP1, chloroplastic [Sesamum indicum]
Length = 632
Score = 199 bits (506), Expect = 1e-54
Identities = 133/317 (41%), Positives = 165/317 (52%), Gaps = 32/317 (10%)
Frame = -2
Query: 858 LKMAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFR------NXXXXXXXXXXKDNQVD 697
L+M V E+R+ PI PV KFGV LA SLGG++YTF R + +Q+D
Sbjct: 6 LQMVVTERRELPIRPVFLKFGVALAISLGGVIYTFSRTKRIKTSKSKSLKLSSGNGSQID 65
Query: 696 SRGESGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC--- 526
G L DD ALQ+ + +V S R S ++D FL E + L+ EC
Sbjct: 66 L---IGALRDDDLALQNTFLQKVASKNSIEDVSSSGRHSGEKDGFLLPEFNELVKECNIS 122
Query: 525 -----------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYGL 415
AEH+E D+E + LR+K+ EYYG+
Sbjct: 123 LTTDGISPMKNEGCLVLDAEKKSAEHDEHDIETRKLRSKIRLLEERERKLEIQLIEYYGI 182
Query: 414 KEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXX 235
KEQE AV+ELQNRLR++NMEAKLYNLKIESL SDNRRLEAQ+AD+ KVV ELE
Sbjct: 183 KEQEAAVIELQNRLRLNNMEAKLYNLKIESLLSDNRRLEAQLADHAKVVMELETAKAKIK 242
Query: 234 XXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXXX 55
SEAEQNREQL L+ERVMKLQ+ EK E+DRDV++
Sbjct: 243 LLRKKFRSEAEQNREQLSTLKERVMKLQELEKKADELDRDVEVYQQEKEELKEELEEMKK 302
Query: 54 XXXXXXXENSELAQKLE 4
ENS+L QKLE
Sbjct: 303 SNEGLKQENSDLTQKLE 319
>ref|XP_022885085.1| protein CHUP1, chloroplastic isoform X2 [Olea europaea var.
sylvestris]
Length = 637
Score = 198 bits (503), Expect = 3e-54
Identities = 139/321 (43%), Positives = 167/321 (52%), Gaps = 40/321 (12%)
Frame = -2
Query: 846 VKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGD 667
VKEKRD I P L KFGV A SLGGI+YT F+N D GES EL +
Sbjct: 2 VKEKRD--IRPELLKFGVAFALSLGGILYTLFKNKKIKPDKSQPPQPSQDVGGESRELEN 59
Query: 666 D---------ARALQDLSVIR-VXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC--- 526
D +R ++S+ + V + R DRD +L E L+ EC
Sbjct: 60 DDEFLHKSPNSRNSPEISLGKYVTSGNSTKDLSPNARYIGDRDGYLLPEFVDLVKECDST 119
Query: 525 ---------------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE 427
EHEE D EI+SL++KV E
Sbjct: 120 VKATGISPRKNMETQALDVESPRGCKYIEHEEPDREIRSLKSKVKMLQERERSLEIQLLE 179
Query: 426 YYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXX 247
YYGLKEQETAVMELQNRL+++NMEAKLYNLKIESLQ++NRRLEA+VADY +VV+ELE
Sbjct: 180 YYGLKEQETAVMELQNRLKLNNMEAKLYNLKIESLQANNRRLEAEVADYAQVVSELEAAK 239
Query: 246 XXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXX 67
L SEAEQNREQ+L LQERVMKLQD+E NGV+ D D+K +
Sbjct: 240 AKIKLLRKKLRSEAEQNREQILTLQERVMKLQDQEMNGVKNDPDMKYKLQKLKELEVEIE 299
Query: 66 XXXXXXXXXXXENSELAQKLE 4
ENSELA+KLE
Sbjct: 300 DLRESNHGLKQENSELAKKLE 320
>ref|XP_022885084.1| protein CHUP1, chloroplastic isoform X1 [Olea europaea var.
sylvestris]
Length = 638
Score = 198 bits (503), Expect = 3e-54
Identities = 139/321 (43%), Positives = 167/321 (52%), Gaps = 40/321 (12%)
Frame = -2
Query: 846 VKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGD 667
VKEKRD I P L KFGV A SLGGI+YT F+N D GES EL +
Sbjct: 2 VKEKRD--IRPELLKFGVAFALSLGGILYTLFKNKKIKPDKSQPPQPSQDVGGESRELEN 59
Query: 666 D---------ARALQDLSVIR-VXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC--- 526
D +R ++S+ + V + R DRD +L E L+ EC
Sbjct: 60 DDEFLHKSPNSRNSPEISLGKYVTSGNSTKDLSPNARYIGDRDGYLLPEFVDLVKECDST 119
Query: 525 ---------------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE 427
EHEE D EI+SL++KV E
Sbjct: 120 VKATGISPRKNMETQALDVESPRGCKYIEHEEPDREIRSLKSKVKMLQERERSLEIQLLE 179
Query: 426 YYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXX 247
YYGLKEQETAVMELQNRL+++NMEAKLYNLKIESLQ++NRRLEA+VADY +VV+ELE
Sbjct: 180 YYGLKEQETAVMELQNRLKLNNMEAKLYNLKIESLQANNRRLEAEVADYAQVVSELEAAK 239
Query: 246 XXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXX 67
L SEAEQNREQ+L LQERVMKLQD+E NGV+ D D+K +
Sbjct: 240 AKIKLLRKKLRSEAEQNREQILTLQERVMKLQDQEMNGVKNDPDMKYKLQKLKELEVEIE 299
Query: 66 XXXXXXXXXXXENSELAQKLE 4
ENSELA+KLE
Sbjct: 300 DLRESNHGLKQENSELAKKLE 320
>gb|EYU45839.1| hypothetical protein MIMGU_mgv1a018903mg, partial [Erythranthe
guttata]
Length = 430
Score = 170 bits (430), Expect = 3e-45
Identities = 114/285 (40%), Positives = 145/285 (50%), Gaps = 14/285 (4%)
Frame = -2
Query: 816 PVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGDDARALQDLSV 637
PV+ K G+ +A SLGGI+YT+ R + E+ L+DL +
Sbjct: 3 PVIMKVGLAIAISLGGILYTYSRTRRIKPSKSKPSQRYFGNGREANSRRQCGNDLKDLPL 62
Query: 636 IRVXXXXXXXXXXXSE-RCSRD-RDNFLFSEVDYLINECAEHEERD------------LE 499
+V + R S D RD FL E D L+ EC + D ++
Sbjct: 63 RKVSSRNSMSEPSSTSGRHSGDHRDGFLLPEFDELLKECITTVKTDDVSQSNKTGDHEID 122
Query: 498 IKSLRNKVXXXXXXXXXXXXXXXEYYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQ 319
I L K+ EYYG+KEQE+AV ELQNRLR++NMEAK+Y+LKIESL
Sbjct: 123 INKLSKKIEILEERERKLEIQLLEYYGIKEQESAVFELQNRLRLNNMEAKIYSLKIESLL 182
Query: 318 SDNRRLEAQVADYEKVVTELEXXXXXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEK 139
SDNRRLE +VADY +VV ELE L SE EQNREQ+L LQ+RVMKLQD+EK
Sbjct: 183 SDNRRLEMKVADYAQVVNELEIAKSKIKMLRKKLKSEGEQNREQILSLQDRVMKLQDQEK 242
Query: 138 NGVEIDRDVKMQXXXXXXXXXXXXXXXXXXXXXXXENSELAQKLE 4
+ DR+V+MQ ENS+LAQKLE
Sbjct: 243 KAADFDREVEMQLKEMNELKAELDEMKKSNGGLKLENSDLAQKLE 287
>ref|XP_012856299.1| PREDICTED: protein CHUP1, chloroplastic [Erythranthe guttata]
Length = 571
Score = 170 bits (430), Expect = 3e-44
Identities = 122/307 (39%), Positives = 162/307 (52%), Gaps = 24/307 (7%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXK------DNQVDSR 691
MAV++KRD I PV+ KFG+ LA S+GGIVYTFFR+ +++VDSR
Sbjct: 1 MAVRDKRDISINPVIIKFGLALALSIGGIVYTFFRSKRIKPSEPKPSLPNPGKNSRVDSR 60
Query: 690 GESGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDR--DNFLFSEVDYLINE---- 529
D RALQD+S+++V S R S + +FL E++ L+ E
Sbjct: 61 E------DIDRALQDISLLKVSPRNSISDNLPSSRRSNEDRGGDFLSHELNQLVREYETA 114
Query: 528 -----------CAEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE-YYGLKEQETAVMEL 385
EE ++EI++LR+KV +Y L+ +E+AV EL
Sbjct: 115 VTTDDVSPGTNMQSLEEHEIEIRNLRSKVEILEEREILLENQLHMCHYDLRAEESAVAEL 174
Query: 384 QNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXXXXXXXLMSEA 205
QNRLR+HNME K YNLKIE+LQ D +LE +VADY VV ELE L +A
Sbjct: 175 QNRLRLHNMEVKQYNLKIETLQLDKIKLEEKVADYANVVKELESAKAKIKMLRKKLTFDA 234
Query: 204 EQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXXXXXXXXXXENS 25
EQNREQ+L L+ERVMK+Q++EK G EI RD+K++ EN
Sbjct: 235 EQNREQILNLKERVMKIQNQEKKGFEISRDLKLE--------EDFEEMKKCNYRLKMENF 286
Query: 24 ELAQKLE 4
ELAQKLE
Sbjct: 287 ELAQKLE 293
>gb|KZV58819.1| hypothetical protein F511_18656 [Dorcoceras hygrometricum]
Length = 610
Score = 170 bits (430), Expect = 6e-44
Identities = 127/321 (39%), Positives = 154/321 (47%), Gaps = 37/321 (11%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXK------DNQVDSR 691
M EKR+ + PVL K GV LA SLGGI+YTF R+ DSR
Sbjct: 1 MVASEKREISLRPVLVKVGVALAVSLGGILYTFLRSRGIKPSKIKPSLPSPDGVTLSDSR 60
Query: 690 GESGELG-DDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC---- 526
ES DD + + S+ + S DR F E L+ EC
Sbjct: 61 RESDWFSVDDCTSKMETSLPEINFLTNQSPSVEY---SGDRYGFFSPEFTKLVKECDLDV 117
Query: 525 --------------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEY 424
++ +E EI SLRN+V EY
Sbjct: 118 TAHSIPPEKNLGTQVLDVELPREFRCSDPDEHSREINSLRNQVEILEERERYLEIQLLEY 177
Query: 423 YGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXX 244
YGLKEQETAV+ELQ++LR+ NMEAKL NLKIESL SDNRRLEAQVADY +VVTELE
Sbjct: 178 YGLKEQETAVVELQDQLRLSNMEAKLGNLKIESLLSDNRRLEAQVADYAEVVTELESAKA 237
Query: 243 XXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXX 64
L EAEQNREQ+L L+ERVMKLQDEEK +EI +D +++
Sbjct: 238 KIKILRKKLRFEAEQNREQILSLRERVMKLQDEEKRAIEIGQDTEIR-RRKENGSEELEE 296
Query: 63 XXXXXXXXXXENSELAQKLEC 1
NSEL++KLEC
Sbjct: 297 VKKSNKDLKLANSELSRKLEC 317
>ref|XP_009595893.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana
tomentosiformis]
Length = 614
Score = 165 bits (417), Expect = 4e-42
Identities = 123/321 (38%), Positives = 154/321 (47%), Gaps = 44/321 (13%)
Frame = -2
Query: 834 RDNP-ITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGDDAR 658
RDN I PVL K GVVL SLGG++YT FR + GE+GEL +D R
Sbjct: 3 RDNRNIRPVLLKIGVVLVLSLGGVIYTIFRTKRIKPSNSFPP--PCSAGGENGELTNDDR 60
Query: 657 AL--------------------QDLSVIRVXXXXXXXXXXXSERCSRDRDN-FLFSEVDY 541
A +DL + ++ S S DRD FL E +
Sbjct: 61 ASHATPRSPSSRKSVSTVSDKHEDLHIAKLIIENSSGVSSSSSIFSNDRDRLFLLPEFNE 120
Query: 540 LINE----------------------CAEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE 427
L+ E E + EIKSL+N V E
Sbjct: 121 LVKELRLSTSKSDIETLMQDSPREYRIVEMVNHEQEIKSLKNIVKTLEERERTLEIQLLE 180
Query: 426 YYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXX 247
YYGLKEQETA+MELQN+L+I+NMEAKL+ LKIESL +D RLEAQVADY KVV+EL+
Sbjct: 181 YYGLKEQETAIMELQNQLKINNMEAKLFGLKIESLSADKMRLEAQVADYAKVVSELDAAK 240
Query: 246 XXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXX 67
L SEA+ ++EQ+L LQE+VMKL DEEK VE + DV+++
Sbjct: 241 VKIKQLKKKLRSEADHSKEQILTLQEKVMKLHDEEKKAVEAESDVQLKLRKLKDLENQAD 300
Query: 66 XXXXXXXXXXXENSELAQKLE 4
ENSELA +LE
Sbjct: 301 ELKKSNHSLRKENSELAHRLE 321
>emb|CDO97961.1| unnamed protein product [Coffea canephora]
Length = 639
Score = 164 bits (414), Expect = 1e-41
Identities = 124/328 (37%), Positives = 156/328 (47%), Gaps = 48/328 (14%)
Frame = -2
Query: 843 KEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKD-------NQVDSRGE 685
+EKRD I PVLFK GV LAFS+G I+++ R NQ DS E
Sbjct: 3 REKRD--IRPVLFKLGVALAFSVGSILFSILRTKKVKPSGSPPSQPSHPEHPNQADSVEE 60
Query: 684 SGELGDDA------RALQDLS-------------VIRVXXXXXXXXXXXSERCSRDRDNF 562
EL +D RA + + + S+R + D+D +
Sbjct: 61 RIELKNDEDDHVLLRATSSCNFALASSERDEPSLLSKTSSGCLSGNCSPSKRSNGDKDVY 120
Query: 561 LFSE----------------------VDYLINECAEHEERDLEIKSLRNKVXXXXXXXXX 448
L E V+ L N C + + + E+ SLRN V
Sbjct: 121 LLPEFNDLVKEFDLAAMKATFSPHKDVEMLTNSCVQKLDHEQELNSLRNMVKTLKERERS 180
Query: 447 XXXXXXEYYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVV 268
EYYGLKEQETAVMEL NRL+I+NMEAKL+NLKIESLQ+D RRLE QVADY +VV
Sbjct: 181 LEIQLLEYYGLKEQETAVMELHNRLKINNMEAKLFNLKIESLQADKRRLEEQVADYARVV 240
Query: 267 TELEXXXXXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXX 88
+ELE L SEAE NRE +L LQERV+KLQD+EK + D D++++
Sbjct: 241 SELEAAKTKIKLLKKKLRSEAEHNREHILALQERVVKLQDQEKKALCTDSDLQLKLQQLK 300
Query: 87 XXXXXXXXXXXXXXXXXXENSELAQKLE 4
ENS LAQKLE
Sbjct: 301 DYEEEAEELRKSNHILRQENSALAQKLE 328
>ref|XP_016483970.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana tabacum]
Length = 614
Score = 161 bits (407), Expect = 1e-40
Identities = 121/321 (37%), Positives = 153/321 (47%), Gaps = 44/321 (13%)
Frame = -2
Query: 834 RDNP-ITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGDDAR 658
RDN + PVL K GVVL SLGG++YT FR + GE+GEL +D R
Sbjct: 3 RDNRNVRPVLLKIGVVLVLSLGGVIYTIFRTKRIKPSNSFPPP--CSAGGENGELTNDDR 60
Query: 657 AL--------------------QDLSVIRVXXXXXXXXXXXSERCSRDRDN-FLFSEVDY 541
A + L + ++ S S DRD FL E +
Sbjct: 61 ASHATPRSPSSRKSVSTVSDKHEGLHIAKLIIENSSGVSSSSGIFSNDRDRLFLLPEFNE 120
Query: 540 LINE----------------------CAEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE 427
L+ E E + EIKSL+N V E
Sbjct: 121 LVKEHRLSTSKSDIETLMQDSPREYRIVEMVNHEQEIKSLKNIVKTLEERERTLEIQLLE 180
Query: 426 YYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXX 247
YYGLKEQETA+MELQN+L+I+NMEAKL+ LKIESL +D RLEAQVADY KVV+EL+
Sbjct: 181 YYGLKEQETAIMELQNQLKINNMEAKLFGLKIESLSADKMRLEAQVADYAKVVSELDAAK 240
Query: 246 XXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXX 67
L SEA+ ++EQ+L LQE+VMKL DEEK VE + DV+++
Sbjct: 241 VKIKQLKKKLRSEADHSKEQILTLQEKVMKLHDEEKKAVEAESDVQLKLRKLKDLENQAD 300
Query: 66 XXXXXXXXXXXENSELAQKLE 4
ENSELA +LE
Sbjct: 301 ELKKSNHSLRKENSELAHRLE 321
>ref|XP_009790396.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana sylvestris]
ref|XP_016500400.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana tabacum]
Length = 621
Score = 161 bits (407), Expect = 1e-40
Identities = 117/320 (36%), Positives = 160/320 (50%), Gaps = 39/320 (12%)
Frame = -2
Query: 846 VKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGE------ 685
+++KRD + PV+ KFGV LA SLGGI++TFFRN + ++GE
Sbjct: 2 MRDKRD--LRPVILKFGVALALSLGGIIFTFFRNKRIKPSNSPPSPSSGGTKGELKNDDQ 59
Query: 684 -----SGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINECA- 523
S D +D S+ + S + DRD FL E + L+ E +
Sbjct: 60 KTPASSNSFFDVPERDEDFSIPKRIIESSISVPSTSGKSDGDRDGFLLPEFNELVKEFSL 119
Query: 522 -----------------------EH----EERDLEIKSLRNKVXXXXXXXXXXXXXXXEY 424
EH ++ + EIK+L+N V EY
Sbjct: 120 SSKANFSPKKDVETPHHDGDSPREHKIVADDHEQEIKNLKNIVKTLKERERTLEIQLLEY 179
Query: 423 YGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXX 244
YGLKEQE+AVMELQNRL+I+NMEAKL LK+ESL+++ RLEA+VADY KVV+ELE
Sbjct: 180 YGLKEQESAVMELQNRLKINNMEAKLLGLKVESLKAEKMRLEAEVADYAKVVSELEAAKL 239
Query: 243 XXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXX 64
L EA+QN+EQ+L L+ERV+KL+D EKN VE + DV+++
Sbjct: 240 KIKQLKKKLRLEADQNKEQILSLKERVLKLRD-EKNPVEAESDVQLKPEKLKDLESEADE 298
Query: 63 XXXXXXXXXXENSELAQKLE 4
ENS LA++LE
Sbjct: 299 LRKFNHSLRVENSTLAERLE 318
>ref|XP_019262213.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana attenuata]
ref|XP_019262214.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana attenuata]
gb|OIT37966.1| protein chup1, chloroplastic [Nicotiana attenuata]
Length = 621
Score = 160 bits (406), Expect = 2e-40
Identities = 118/320 (36%), Positives = 159/320 (49%), Gaps = 39/320 (12%)
Frame = -2
Query: 846 VKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGE------ 685
+++KRD I PV+ KFGV LA SLGGI++TFFRN + ++GE
Sbjct: 2 MRDKRD--IRPVILKFGVALALSLGGIIFTFFRNKRIKPSNSSPSPSSGGTKGELKNDDQ 59
Query: 684 -----SGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINECA- 523
S D +D S+ + S + DRD FL E + ++ E +
Sbjct: 60 KTPASSNSFFDVPERDEDFSIPKRIIESSISVPSTSCKSDGDRDGFLLPEFNEIVKEFSL 119
Query: 522 -----------------------EH----EERDLEIKSLRNKVXXXXXXXXXXXXXXXEY 424
EH + + EIK+L+N V EY
Sbjct: 120 SSKANFSPKKDVETPQQDADSPREHKIVADNHEQEIKNLKNIVKTLKERERTLEIQLLEY 179
Query: 423 YGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXX 244
YGLKEQE+AVMELQNRL+I+NMEAKL LK+ESL+++ RLEAQVADY KVV+ELE
Sbjct: 180 YGLKEQESAVMELQNRLKINNMEAKLLGLKVESLKAEKTRLEAQVADYAKVVSELEAAKL 239
Query: 243 XXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXX 64
L EA+QN+EQ+L L+ERV+KL+D EKN VE + DV+++
Sbjct: 240 KIKQLKKKLRLEADQNKEQILSLKERVLKLRD-EKNPVEAESDVQLKPEKLKDWESEADE 298
Query: 63 XXXXXXXXXXENSELAQKLE 4
ENS LA++LE
Sbjct: 299 LRKFNHSLIVENSTLAERLE 318
>ref|XP_019243552.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana attenuata]
gb|OIT04792.1| protein chup1, chloroplastic [Nicotiana attenuata]
Length = 616
Score = 160 bits (404), Expect = 3e-40
Identities = 119/322 (36%), Positives = 151/322 (46%), Gaps = 45/322 (13%)
Frame = -2
Query: 834 RDNP-ITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGDDAR 658
RDN I PVL K G VL SLGG++YT FR + GE+GEL +D
Sbjct: 3 RDNRNIRPVLLKIGAVLVLSLGGVIYTIFRT--KRIKPSNLFSPPCSTGGENGELTNDDH 60
Query: 657 AL--------------------QDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYL 538
A +DL + + S + +RD FL E + L
Sbjct: 61 ASHATPISPSSRKSVSTVSDKHEDLHICKHIIENSTALPSSSGMFNSNRDGFLLPEFNEL 120
Query: 537 INE------------------------CAEHEERDLEIKSLRNKVXXXXXXXXXXXXXXX 430
+ E E + EIKSL+N V
Sbjct: 121 VKELRLSTSKRDIETLLQYEDSPREYRIVEMVNHEQEIKSLKNIVKTLEERERTLEIQLL 180
Query: 429 EYYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXX 250
EYYGLKEQETA+MELQN+L+I+NMEAKL+ LKIESL +D RLEAQVADY KVV+EL+
Sbjct: 181 EYYGLKEQETAIMELQNQLKINNMEAKLFGLKIESLTADKMRLEAQVADYAKVVSELDAA 240
Query: 249 XXXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXX 70
L SEA+ ++EQ+L LQE+VMKL DEEK VE + DV+++
Sbjct: 241 KVKIKQLKKKLRSEADHSKEQILTLQEKVMKLHDEEKKAVEAESDVQLKLRKLKDLENQA 300
Query: 69 XXXXXXXXXXXXENSELAQKLE 4
ENSELA +LE
Sbjct: 301 DELKKSNHSLRKENSELAHRLE 322
>ref|XP_009590753.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana
tomentosiformis]
ref|XP_016443826.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana tabacum]
Length = 621
Score = 157 bits (397), Expect = 3e-39
Identities = 119/323 (36%), Positives = 160/323 (49%), Gaps = 42/323 (13%)
Frame = -2
Query: 846 VKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXKDNQVDSRGESGELGD 667
+++KRD + PV+ KFGV LA SLGGI++ FFR N S G+ G+L +
Sbjct: 2 MRDKRD--LRPVILKFGVALALSLGGILFAFFRTKRIKPSNSSPSPN---SGGQKGKLKN 56
Query: 666 DARAL--------------QDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINE 529
D + +D S+ + S R DRD FL E + L+ E
Sbjct: 57 DDQKTPASCNSVFDVPERDEDFSLPKRIIESSISGPSTSGRSDGDRDGFLLPEFNELVEE 116
Query: 528 CA------------------------EH----EERDLEIKSLRNKVXXXXXXXXXXXXXX 433
+ EH ++ + EIK+L+N V
Sbjct: 117 FSLFSKANFSPKKDVETPQHDGDSPREHKIVADDHEQEIKNLKNIVKTLKERERTLEIQL 176
Query: 432 XEYYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEX 253
EYYGLKEQE+AVMELQNRL+I+NMEAKL LK ESL+++ RLEAQVADY KVV+ELE
Sbjct: 177 LEYYGLKEQESAVMELQNRLKINNMEAKLLGLKAESLKAEKTRLEAQVADYAKVVSELEA 236
Query: 252 XXXXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXX 73
L EA+QN+EQ+L L+ERV+KL+D EKN VE + DV+++
Sbjct: 237 AKLKIKQLKKKLRLEADQNKEQILSLKERVLKLRD-EKNPVEAESDVQLKPEKLKDLESE 295
Query: 72 XXXXXXXXXXXXXENSELAQKLE 4
ENS LA++LE
Sbjct: 296 ADELRKFNHSLRVENSTLAERLE 318
>ref|XP_012836445.1| PREDICTED: protein CHUP1, chloroplastic-like [Erythranthe guttata]
Length = 500
Score = 152 bits (385), Expect = 3e-38
Identities = 93/202 (46%), Positives = 113/202 (55%), Gaps = 12/202 (5%)
Frame = -2
Query: 573 RDNFLFSEVDYLINECAEHEERD------------LEIKSLRNKVXXXXXXXXXXXXXXX 430
RD FL E D L+ EC + D ++I L K+
Sbjct: 16 RDGFLLPEFDELLKECITTVKTDDVSQSNKTGDHEIDINKLSKKIEILEERERKLEIQLL 75
Query: 429 EYYGLKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXX 250
EYYG+KEQE+AV ELQNRLR++NMEAK+Y+LKIESL SDNRRLE +VADY +VV ELE
Sbjct: 76 EYYGIKEQESAVFELQNRLRLNNMEAKIYSLKIESLLSDNRRLEMKVADYAQVVNELEIA 135
Query: 249 XXXXXXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXX 70
L SE EQNREQ+L LQ+RVMKLQD+EK + DR+V+MQ
Sbjct: 136 KSKIKMLRKKLKSEGEQNREQILSLQDRVMKLQDQEKKAADFDREVEMQLKEMNELKAEL 195
Query: 69 XXXXXXXXXXXXENSELAQKLE 4
ENS+LAQKLE
Sbjct: 196 DEMKKSNGGLKLENSDLAQKLE 217
>ref|XP_009790609.1| PREDICTED: protein CHUP1, chloroplastic-like isoform X1 [Nicotiana
sylvestris]
ref|XP_016446681.1| PREDICTED: protein CHUP1, chloroplastic-like [Nicotiana tabacum]
Length = 626
Score = 152 bits (384), Expect = 2e-37
Identities = 118/318 (37%), Positives = 150/318 (47%), Gaps = 41/318 (12%)
Frame = -2
Query: 834 RDNP-ITPV-LFKFGVVLAFSLGGIVYTFFR-------NXXXXXXXXXXKDNQVDSRGES 682
RDN I PV L K GVVL SLGGI+YT FR N + D +
Sbjct: 3 RDNRNIRPVVLLKIGVVLVVSLGGIIYTIFRTKRIKPSNSSPPPCSTGVELTNDDHASHA 62
Query: 681 GELGDDARAL--------QDLSVIRVXXXXXXXXXXXSERCSRDRDNFLFSEVDYLINEC 526
+ +R +DL + + S + +RD FL E + L+ E
Sbjct: 63 APISPSSRKSVSTVPDKNEDLHICKHIIENSTPLPSSSGIFNSNRDGFLLPEFNELVKEL 122
Query: 525 ------------------------AEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXEYYG 418
E D EIKSL+N V EYYG
Sbjct: 123 RLSTSKREIEMLLQYEDSPREYRIVEMVNHDQEIKSLKNIVKTLEEREKNLEIQLLEYYG 182
Query: 417 LKEQETAVMELQNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXX 238
LKEQETA+MELQN+L+I+NMEAKL++LKIESL +D RLEAQVADY KVV+EL+
Sbjct: 183 LKEQETAIMELQNQLKINNMEAKLFDLKIESLSADKLRLEAQVADYAKVVSELDAAKVKI 242
Query: 237 XXXXXXLMSEAEQNREQLLYLQERVMKLQDEEKNGVEIDRDVKMQXXXXXXXXXXXXXXX 58
L SEA+ ++EQ+L LQE+VMKL DEEK VE + DV+++
Sbjct: 243 KQLKKKLRSEADHSKEQILTLQEKVMKLHDEEKKAVEAESDVQLKLRKLKDLENQADELK 302
Query: 57 XXXXXXXXENSELAQKLE 4
ENSELA +LE
Sbjct: 303 KSNHSLRKENSELAHRLE 320
>gb|EYU21441.1| hypothetical protein MIMGU_mgv1a004777mg [Erythranthe guttata]
Length = 511
Score = 149 bits (375), Expect = 9e-37
Identities = 103/262 (39%), Positives = 141/262 (53%), Gaps = 24/262 (9%)
Frame = -2
Query: 852 MAVKEKRDNPITPVLFKFGVVLAFSLGGIVYTFFRNXXXXXXXXXXK------DNQVDSR 691
MAV++KRD I PV+ KFG+ LA S+GGIVYTFFR+ +++VDSR
Sbjct: 1 MAVRDKRDISINPVIIKFGLALALSIGGIVYTFFRSKRIKPSEPKPSLPNPGKNSRVDSR 60
Query: 690 GESGELGDDARALQDLSVIRVXXXXXXXXXXXSERCSRDR--DNFLFSEVDYLINE---- 529
D RALQD+S+++V S R S + +FL E++ L+ E
Sbjct: 61 E------DIDRALQDISLLKVSPRNSISDNLPSSRRSNEDRGGDFLSHELNQLVREYETA 114
Query: 528 -----------CAEHEERDLEIKSLRNKVXXXXXXXXXXXXXXXE-YYGLKEQETAVMEL 385
EE ++EI++LR+KV +Y L+ +E+AV EL
Sbjct: 115 VTTDDVSPGTNMQSLEEHEIEIRNLRSKVEILEEREILLENQLHMCHYDLRAEESAVAEL 174
Query: 384 QNRLRIHNMEAKLYNLKIESLQSDNRRLEAQVADYEKVVTELEXXXXXXXXXXXXLMSEA 205
QNRLR+HNME K YNLKIE+LQ D +LE +VADY VV ELE L +A
Sbjct: 175 QNRLRLHNMEVKQYNLKIETLQLDKIKLEEKVADYANVVKELESAKAKIKMLRKKLTFDA 234
Query: 204 EQNREQLLYLQERVMKLQDEEK 139
EQNREQ+L L+ER+ L++E +
Sbjct: 235 EQNREQILNLKERIQALKEENQ 256