BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia30_contig00014567
         (2941 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branchin...  1375   0.0  
ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum...  1360   0.0  
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...  1284   0.0  
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea...  1239   0.0  
ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea...  1215   0.0  
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]       1206   0.0  
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...  1179   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                            1125   0.0  
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1097   0.0  
ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Sola...  1097   0.0  
ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [...  1097   0.0  
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] >gi...  1095   0.0  
ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1091   0.0  
ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1090   0.0  
ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1088   0.0  
ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Sola...  1088   0.0  
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1087   0.0  
gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]       1086   0.0  
gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]         1083   0.0  
ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1083   0.0  

>gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
            [Handroanthus impetiginosus]
          Length = 853

 Score = 1375 bits (3558), Expect = 0.0
 Identities = 678/857 (79%), Positives = 749/857 (87%), Gaps = 1/857 (0%)
 Frame = -1

Query: 2707 MLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPARRI 2528
            MLP+P+V QS CLS EA ++SK FA S G+  +RVTS+S KL+LKQ+ V GQLMKP RRI
Sbjct: 1    MLPMPHVTQSQCLSSEAFDASKFFATSHGKYRKRVTSNSQKLDLKQRRVFGQLMKPFRRI 60

Query: 2527 SYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFLEH 2348
            S +HHVLKALATSDVSVV+ SENVATYRF ME GGQLKV +KKK+NNK+ L+VEVE L+ 
Sbjct: 61   SCNHHVLKALATSDVSVVETSENVATYRFRMETGGQLKVLVKKKNNNKHVLYVEVESLQL 120

Query: 2347 CEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDESL 2168
              GQ ELF++WGLFR  +T FMPL F++SSAD   +TIETPF K+S    AVE+DFDE+L
Sbjct: 121  LGGQRELFLVWGLFRDHATTFMPLGFQKSSADD--NTIETPFMKDSSDRFAVEVDFDENL 178

Query: 2167 APFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAFFS 1988
             PFYVS LLKSQ GS+LK+STIRSHRKTNFIVPVGFGSGRP PLGISF DDG  NFAFFS
Sbjct: 179  PPFYVSFLLKSQCGSDLKISTIRSHRKTNFIVPVGFGSGRPFPLGISFLDDGSTNFAFFS 238

Query: 1987 RNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTENK 1808
            +N ESVVLCLY+DT  D+PALEIDLDPYVNR+GDIWHA VDCS  FV YGYRCR+G +NK
Sbjct: 239  QNAESVVLCLYSDTTVDEPALEIDLDPYVNRTGDIWHASVDCSPHFVSYGYRCRSGPDNK 298

Query: 1807 GHRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFT 1628
            GH VLLDPYA+VIED  LDLP+++LGKL +EP FDWSG+F PS+PME+LIIYRLNVTRFT
Sbjct: 299  GHNVLLDPYAKVIEDIGLDLPQRYLGKLFKEPPFDWSGEFCPSIPMEKLIIYRLNVTRFT 358

Query: 1627 IDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLY 1448
               SSKLPGDIAG+F+G+SEKLHHFK+LGVNAVLLEPIFPFDE+K PYFP HFFSPG+LY
Sbjct: 359  RHNSSKLPGDIAGSFSGVSEKLHHFKDLGVNAVLLEPIFPFDEKKRPYFPAHFFSPGNLY 418

Query: 1447 GPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLK 1268
            GPSG+P +VAKSMKEMVKKLHASGIEVLLEVVFTHT E  SLREIDNS YY + GGEDLK
Sbjct: 419  GPSGNPNSVAKSMKEMVKKLHASGIEVLLEVVFTHTGETGSLREIDNSCYYHIKGGEDLK 478

Query: 1267 SRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAF 1088
              NALNCNYPIVQQMILESLRYWV EFHIDGFCF+NASSLTRG HGEFLSRPPL+EAIAF
Sbjct: 479  LGNALNCNYPIVQQMILESLRYWVTEFHIDGFCFINASSLTRGFHGEFLSRPPLVEAIAF 538

Query: 1087 DPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRL 908
            DPLLSKVK+IADSWDPH +ETKE AFPHWKRWAEMN +FCVDVRNFLRG+GPI+NLATRL
Sbjct: 539  DPLLSKVKIIADSWDPHDMETKEIAFPHWKRWAEMNARFCVDVRNFLRGKGPINNLATRL 598

Query: 907  CGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLE 728
            CGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFS SDLE + SWNCGEEGPTNK+VVLE
Sbjct: 599  CGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSNSDLESELSWNCGEEGPTNKSVVLE 658

Query: 727  TRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQF 548
             RLKQIRNFLF+LFISLGVPVLNMGDECGQS+GGS  Y DRK LDWN LKSGFGIQVTQF
Sbjct: 659  RRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGSTAYADRKLLDWNALKSGFGIQVTQF 718

Query: 547  ISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQST 368
            ISFLSSL++RRSDLLQ RSFLKE+NIEW GT+Q PP WDD SCKFL+MTLK +A P  S 
Sbjct: 719  ISFLSSLRIRRSDLLQKRSFLKEDNIEWHGTQQSPPTWDDPSCKFLAMTLKAEAPPLSSP 778

Query: 367  SGSLNAGGDLFIAFNSGDHSEKVTLPPLA-VDATWVCLVDTALPFPGFFTVDGVPLEAGL 191
            +     GGDLFIAFNS DHS KV +PPLA VDATWV LVDTALP PGFF+ DGVPL+AGL
Sbjct: 779  N---VIGGDLFIAFNSADHSAKVAVPPLASVDATWVRLVDTALPLPGFFSKDGVPLDAGL 835

Query: 190  ATYEMKSHSCILFEARS 140
             TYEMKSHSCILFEARS
Sbjct: 836  VTYEMKSHSCILFEARS 852


>ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum]
 ref|XP_011092270.1| isoamylase 2, chloroplastic [Sesamum indicum]
          Length = 857

 Score = 1360 bits (3521), Expect = 0.0
 Identities = 666/858 (77%), Positives = 742/858 (86%), Gaps = 1/858 (0%)
 Frame = -1

Query: 2707 MLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNL-KQKTVHGQLMKPARR 2531
            M PVP +MQSHC S   +  +KLF+A+CG  G+R+ S   +L++ KQ   +G+ +K  RR
Sbjct: 1    MHPVPCLMQSHCPSSGIVGPTKLFSANCGNYGKRLVSGPKRLDVAKQAMFNGRQVKYVRR 60

Query: 2530 ISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFLE 2351
            ISY  HVLK++A SDVSVVK SEN+ATYRF MEIGGQL+  +K+KSNNKYGL+VEVE  +
Sbjct: 61   ISYGPHVLKSVANSDVSVVKASENMATYRFWMEIGGQLRALVKRKSNNKYGLYVEVESSQ 120

Query: 2350 HCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDES 2171
              EG  EL MIWGLFR+DST FMPLDF++SSADSK STIETPF K+S G LAVELDF+ES
Sbjct: 121  LYEGHSELVMIWGLFRADSTNFMPLDFQRSSADSKYSTIETPFVKDSNGRLAVELDFEES 180

Query: 2170 LAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAFF 1991
            LAPFYVSILLKS++GSN K+STIRS RKTNFIVPVGFGSG PSPLGISF+D+G INFAFF
Sbjct: 181  LAPFYVSILLKSKIGSNSKISTIRSLRKTNFIVPVGFGSGYPSPLGISFSDNGFINFAFF 240

Query: 1990 SRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTEN 1811
            SR+ E VVLCLY D   +KPALEIDLDPYVNRSGDIWHA +D S+PFV YGYRCR+ T+N
Sbjct: 241  SRDAERVVLCLYMDNTLEKPALEIDLDPYVNRSGDIWHASIDSSMPFVSYGYRCRSATDN 300

Query: 1810 KGHRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRF 1631
            KGH VLLDPYA+VIED  L LPRK LGKL +EPA+DWSG+F PSLPME+LIIYRLN+T F
Sbjct: 301  KGHHVLLDPYAKVIEDLGLGLPRKCLGKLYKEPAYDWSGEFHPSLPMEKLIIYRLNITGF 360

Query: 1630 TIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSL 1451
            T +KSSKLPGDIAGTF+G+SEKLHHFK+LGVNA+LLEPIFPFDE+KGPYFPWHFFSP  L
Sbjct: 361  TKNKSSKLPGDIAGTFSGLSEKLHHFKDLGVNAILLEPIFPFDEKKGPYFPWHFFSPERL 420

Query: 1450 YGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDL 1271
             GP+GDP TVA+SMKEMVKKLH SGIEVLLEVVFTHT E  +LR+ID SSYY   GG+DL
Sbjct: 421  CGPTGDPTTVAQSMKEMVKKLHGSGIEVLLEVVFTHTGEGCALRDID-SSYYHTEGGDDL 479

Query: 1270 KSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIA 1091
             SRNALNCNYPIVQQM+LESLRYWVIEFHIDGFCF+NAS L RG HGEFLSRPPL+EAIA
Sbjct: 480  TSRNALNCNYPIVQQMVLESLRYWVIEFHIDGFCFINASHLMRGFHGEFLSRPPLVEAIA 539

Query: 1090 FDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATR 911
            FDP+LSK K+IADSWDPH +ETKE  FPHWKRWAEMN KFCVDVRNFLRGQGPISNLATR
Sbjct: 540  FDPVLSKAKIIADSWDPHEMETKEIVFPHWKRWAEMNAKFCVDVRNFLRGQGPISNLATR 599

Query: 910  LCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVL 731
            LCGSGDLFL GRGPAFS NFITRNVGLTLVDLVSFS S    + SWNCGEEGPTNKT+VL
Sbjct: 600  LCGSGDLFLGGRGPAFSINFITRNVGLTLVDLVSFSSSTFGSELSWNCGEEGPTNKTLVL 659

Query: 730  ETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQ 551
            E RLKQIRNFLF+LFISLGVPVLNMGDECGQSTGGS  Y DR+PLDWN L+SGFG QVTQ
Sbjct: 660  ERRLKQIRNFLFILFISLGVPVLNMGDECGQSTGGSQEYADRRPLDWNALRSGFGTQVTQ 719

Query: 550  FISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQS 371
            FISFLS L+ RRSD+LQ RSFLKE+NIEW GT++ PP WDD SCKFL+MTLK DA PSQ 
Sbjct: 720  FISFLSLLRRRRSDILQRRSFLKEDNIEWHGTDRTPPMWDDPSCKFLAMTLKADALPSQV 779

Query: 370  TSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGL 191
            TSGS NAGGDLF+AFNS +H +KV LP LAV +TWV LVDTALPFPGFFTVDGVPL+AGL
Sbjct: 780  TSGSPNAGGDLFVAFNSANHLQKVALPQLAVGSTWVRLVDTALPFPGFFTVDGVPLDAGL 839

Query: 190  ATYEMKSHSCILFEARSL 137
            ATYEMKSHSCILFEAR L
Sbjct: 840  ATYEMKSHSCILFEARKL 857


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842326.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842327.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842328.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
          Length = 840

 Score = 1284 bits (3323), Expect = 0.0
 Identities = 643/862 (74%), Positives = 720/862 (83%), Gaps = 4/862 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHG-QLMKPA 2537
            MA+LPVPYVMQ HC+  E+ ESSK F  S G  G+RVTSDS KL+ ++  +HG Q  KP 
Sbjct: 1    MAVLPVPYVMQPHCVGNESFESSKSFPTSHGRYGKRVTSDSRKLDFRRINLHGGQFAKPI 60

Query: 2536 RRISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEF 2357
             RIS  HHV KA+ATSDVS VK+ ENV TYRF MEIGGQLKV +KK +NNKYG++VEVE 
Sbjct: 61   GRISCSHHVTKAIATSDVSAVKILENVLTYRFWMEIGGQLKVMVKKNNNNKYGVYVEVES 120

Query: 2356 LEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFD 2177
            L+  E Q EL MIWG+FR D T FMP+  +QSS D    T ETPF K+    L+VEL+F+
Sbjct: 121  LQLDEEQRELIMIWGIFRDDLTKFMPV--QQSSTDG---TTETPFVKDC-DRLSVELEFE 174

Query: 2176 ESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFA 1997
            ESLAPFYVSILLKS+LGSN K STIRSHRKTNFIVPVGF SGRPSPLGIS  +DG INFA
Sbjct: 175  ESLAPFYVSILLKSKLGSNSKTSTIRSHRKTNFIVPVGFRSGRPSPLGISLLNDGFINFA 234

Query: 1996 FFSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCS-LPFVGYGYRCRNG 1820
            FFSRN  SVVLCLYADT  DKPALEIDLDPYVNRSGDIWHA +DCS LPFV YGYRCR+ 
Sbjct: 235  FFSRNAYSVVLCLYADTTVDKPALEIDLDPYVNRSGDIWHASIDCSSLPFVSYGYRCRSS 294

Query: 1819 -TENKGHRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYRLN 1643
             T++KGHRVL DPYA+VIE+F    PRK LG+LC E AFDWSG+ RP+LPME+LI+YRLN
Sbjct: 295  STDSKGHRVLPDPYAKVIEEFG---PRKCLGRLCNESAFDWSGESRPNLPMEKLIVYRLN 351

Query: 1642 VTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFS 1463
            V RFT DKSSKLP DIAG+F+GIS+KLH+FKNLGVNAVLLEPIFPFDEQKGPYFPWHFFS
Sbjct: 352  VARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFS 411

Query: 1462 PGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSVNG 1283
            PGSLYGPSGDP  VA S+KEMVKKLHA+G+EVLLEVVFTHTSE+++LREIDNSSY+ V G
Sbjct: 412  PGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVVFTHTSEISALREIDNSSYHHVEG 471

Query: 1282 GEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPPLI 1103
            GE+LKSRN+LNCNYPIVQQMILE LRYWV EFHIDGFCF+N+SSLTRG HGEFLSRPPL+
Sbjct: 472  GEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLTRGFHGEFLSRPPLV 531

Query: 1102 EAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPISN 923
            EAIAFDPLLSKVK++ADSWDPH +E KE  FPHWKRWAEMN KFC ++RNF RG+G IS+
Sbjct: 532  EAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPHWKRWAEMNAKFCSEMRNFFRGKGSISS 591

Query: 922  LATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNK 743
            LATRLCGSGDLFL GR PAFSFNF+TRNVG TLVDLVS++      + SWNCGEEGPTNK
Sbjct: 592  LATRLCGSGDLFLGGRSPAFSFNFVTRNVGPTLVDLVSYNSI----ESSWNCGEEGPTNK 647

Query: 742  TVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGFGI 563
             +VLETRLKQIRNF+FVLF+SLGVPVLNMGDECGQST GSP Y+DR   DW+ LKSGFG 
Sbjct: 648  ILVLETRLKQIRNFIFVLFVSLGVPVLNMGDECGQSTRGSPEYSDRISFDWSALKSGFGT 707

Query: 562  QVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTDAQ 383
            QVT+FISFLSSL++RR+DLLQ RSFLKE+NIEW G E  PP WDD SCKFL+MTLK D  
Sbjct: 708  QVTEFISFLSSLRIRRNDLLQKRSFLKEDNIEWHGAEGGPPMWDDPSCKFLAMTLKADPP 767

Query: 382  PSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPL-AVDATWVCLVDTALPFPGFFTVDGVP 206
             S           DLF+AFN GD SEKV LPPL AVD  WVCLVDT+LPFPGFFT +GVP
Sbjct: 768  LS-----------DLFVAFNGGDCSEKVALPPLAAVDTNWVCLVDTSLPFPGFFTEEGVP 816

Query: 205  LEAGLATYEMKSHSCILFEARS 140
            LE GL TYEMKSHSC+LFEARS
Sbjct: 817  LEDGLETYEMKSHSCVLFEARS 838


>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
            sylvestris]
          Length = 849

 Score = 1239 bits (3207), Expect = 0.0
 Identities = 613/850 (72%), Positives = 707/850 (83%)
 Frame = -1

Query: 2686 MQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVL 2507
            MQS CLS+ ALE SK F+ASC +  + VT    K++ K K V   L K  RRIS++ H  
Sbjct: 5    MQSQCLSLGALELSK-FSASCCKYDKGVTCGFQKMDAKYKQVLSGLGKSVRRISHELHTW 63

Query: 2506 KALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGEL 2327
            KALATSDVSV + +E VA YRF  E GGQLK+ ++K +N KYG++VEV    H   +G+L
Sbjct: 64   KALATSDVSV-QTAEKVAIYRFRTENGGQLKLLVRK-NNKKYGVYVEVLSPLHSGRKGDL 121

Query: 2326 FMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSI 2147
             M WGLFRS+S  FMP+DF+ SSADSK STI+TPF K+S G L VELDFDESLAPFY+S+
Sbjct: 122  LMSWGLFRSNSESFMPMDFENSSADSKYSTIDTPFVKDSSGRLVVELDFDESLAPFYISV 181

Query: 2146 LLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVV 1967
            LLKS LGS+L+ S IRSHRKTNFIVPVGF SGRP+PLG+S   +G +NFAFFSRN ESVV
Sbjct: 182  LLKS-LGSDLESSEIRSHRKTNFIVPVGFRSGRPTPLGLSILANGSMNFAFFSRNAESVV 240

Query: 1966 LCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLD 1787
            LCLYADT A+KPALEIDLDPYVNRSGDIWH L+D S+PFV YGYRC++  + K   VLLD
Sbjct: 241  LCLYADTTANKPALEIDLDPYVNRSGDIWHVLLDGSVPFVRYGYRCKSNADKKRQYVLLD 300

Query: 1786 PYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKL 1607
            PYA++IEDF   LPRK LGKL +E  FDWSGD RPSLPME+LIIYRLNVTRFT D+SSKL
Sbjct: 301  PYAKLIEDFGSGLPRKSLGKLWKESGFDWSGDMRPSLPMEKLIIYRLNVTRFTKDESSKL 360

Query: 1606 PGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPK 1427
            PG+I GTFAGISEK+HHFK+LGVNA+LLEPIFPFDEQ GPYFPWHFFSPGS YGPSGD +
Sbjct: 361  PGEIVGTFAGISEKVHHFKDLGVNAILLEPIFPFDEQTGPYFPWHFFSPGSPYGPSGDSE 420

Query: 1426 TVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNC 1247
             V K MKEMVKKLH +GIEVL+EVVFTHT+E+A+L++ID S YY V GGEDLKSRNALNC
Sbjct: 421  FVVKLMKEMVKKLHLNGIEVLVEVVFTHTAEVAALKQIDRS-YYHVGGGEDLKSRNALNC 479

Query: 1246 NYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKV 1067
            N+PIVQQ+IL+SLR+WVIE+HIDGFCF++ASSL +G HGEFL+RPPL+EAIAFDPLLSKV
Sbjct: 480  NHPIVQQLILDSLRHWVIEYHIDGFCFIDASSLMKGFHGEFLTRPPLVEAIAFDPLLSKV 539

Query: 1066 KLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLF 887
            K+IADSWDPH++ETK+ +FPHWKRWAE+N KFC DVRNFLRGQG I NLATRLCGSG+LF
Sbjct: 540  KIIADSWDPHNMETKDISFPHWKRWAEINSKFCNDVRNFLRGQGSIGNLATRLCGSGNLF 599

Query: 886  LSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIR 707
            L GRGPAFSFNFI+RN GL LVDLV FS S+L  + SWNCGEEGPTNK  VLE RLKQIR
Sbjct: 600  LDGRGPAFSFNFISRNAGLPLVDLVCFSSSELASELSWNCGEEGPTNKNAVLERRLKQIR 659

Query: 706  NFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSL 527
            N+LF+LF SLGVPVLNMGDECGQS+GGSP + DRKPLDWN LKSGF IQ T+FI+FLSSL
Sbjct: 660  NYLFILFFSLGVPVLNMGDECGQSSGGSPAHADRKPLDWNALKSGFAIQTTKFITFLSSL 719

Query: 526  KVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAG 347
            K+RRSDLLQNR+F KEENIEW G++  PP+WD+ SC+FL+MTLK D++ SQ TSG     
Sbjct: 720  KMRRSDLLQNRNFWKEENIEWHGSDGSPPRWDEPSCRFLAMTLKADSKESQPTSGLHEMI 779

Query: 346  GDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSH 167
            GDLFIAFNS   SE + LPPL  D  W  LVDT+LPFPGFFT DGVPL+ G  TYEMKSH
Sbjct: 780  GDLFIAFNSAGRSENIVLPPLTEDIAWFRLVDTSLPFPGFFTTDGVPLKDGSVTYEMKSH 839

Query: 166  SCILFEARSL 137
            SC+LFEARSL
Sbjct: 840  SCVLFEARSL 849


>ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea europaea var.
            sylvestris]
          Length = 829

 Score = 1215 bits (3144), Expect = 0.0
 Identities = 596/817 (72%), Positives = 686/817 (83%)
 Frame = -1

Query: 2587 KLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVF 2408
            K++ K K V   L K  RRIS++ H  KALATSDVSV + +E VA YRF  E GGQLK+ 
Sbjct: 17   KMDAKYKQVLSGLGKSVRRISHELHTWKALATSDVSV-QTAEKVAIYRFRTENGGQLKLL 75

Query: 2407 IKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIET 2228
            ++K +N KYG++VEV    H   +G+L M WGLFRS+S  FMP+DF+ SSADSK STI+T
Sbjct: 76   VRK-NNKKYGVYVEVLSPLHSGRKGDLLMSWGLFRSNSESFMPMDFENSSADSKYSTIDT 134

Query: 2227 PFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGR 2048
            PF K+S G L VELDFDESLAPFY+S+LLKS LGS+L+ S IRSHRKTNFIVPVGF SGR
Sbjct: 135  PFVKDSSGRLVVELDFDESLAPFYISVLLKS-LGSDLESSEIRSHRKTNFIVPVGFRSGR 193

Query: 2047 PSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALV 1868
            P+PLG+S   +G +NFAFFSRN ESVVLCLYADT A+KPALEIDLDPYVNRSGDIWH L+
Sbjct: 194  PTPLGLSILANGSMNFAFFSRNAESVVLCLYADTTANKPALEIDLDPYVNRSGDIWHVLL 253

Query: 1867 DCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDF 1688
            D S+PFV YGYRC++  + K   VLLDPYA++IEDF   LPRK LGKL +E  FDWSGD 
Sbjct: 254  DGSVPFVRYGYRCKSNADKKRQYVLLDPYAKLIEDFGSGLPRKSLGKLWKESGFDWSGDM 313

Query: 1687 RPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFP 1508
            RPSLPME+LIIYRLNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNA+LLEPIFP
Sbjct: 314  RPSLPMEKLIIYRLNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAILLEPIFP 373

Query: 1507 FDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELA 1328
            FDEQ GPYFPWHFFSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFTHT+E+A
Sbjct: 374  FDEQTGPYFPWHFFSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFTHTAEVA 433

Query: 1327 SLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSL 1148
            +L++ID S YY V GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF++ASSL
Sbjct: 434  ALKQIDRS-YYHVGGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCFIDASSL 492

Query: 1147 TRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFC 968
             +G HGEFL+RPPL+EAIAFDPLLSKVK+IADSWDPH++ETK+ +FPHWKRWAE+N KFC
Sbjct: 493  MKGFHGEFLTRPPLVEAIAFDPLLSKVKIIADSWDPHNMETKDISFPHWKRWAEINSKFC 552

Query: 967  VDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLE 788
             DVRNFLRGQG I NLATRLCGSG+LFL GRGPAFSFNFI+RN GL LVDLV FS S+L 
Sbjct: 553  NDVRNFLRGQGSIGNLATRLCGSGNLFLDGRGPAFSFNFISRNAGLPLVDLVCFSSSELA 612

Query: 787  PQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTD 608
             + SWNCGEEGPTNK  VLE RLKQIRN+LF+LF SLGVPVLNMGDECGQS+GGSP + D
Sbjct: 613  SELSWNCGEEGPTNKNAVLERRLKQIRNYLFILFFSLGVPVLNMGDECGQSSGGSPAHAD 672

Query: 607  RKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDD 428
            RKPLDWN LKSGF IQ T+FI+FLSSLK+RRSDLLQNR+F KEENIEW G++  PP+WD+
Sbjct: 673  RKPLDWNALKSGFAIQTTKFITFLSSLKMRRSDLLQNRNFWKEENIEWHGSDGSPPRWDE 732

Query: 427  ESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDT 248
             SC+FL+MTLK D++ SQ TSG     GDLFIAFNS   SE + LPPL  D  W  LVDT
Sbjct: 733  PSCRFLAMTLKADSKESQPTSGLHEMIGDLFIAFNSAGRSENIVLPPLTEDIAWFRLVDT 792

Query: 247  ALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 137
            +LPFPGFFT DGVPL+ G  TYEMKSHSC+LFEARSL
Sbjct: 793  SLPFPGFFTTDGVPLKDGSVTYEMKSHSCVLFEARSL 829


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score = 1206 bits (3119), Expect = 0.0
 Identities = 605/857 (70%), Positives = 685/857 (79%)
 Frame = -1

Query: 2707 MLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPARRI 2528
            MLPV Y     C S  A ESS LFAA  G+    VT D  K +LKQK    +L K   RI
Sbjct: 1    MLPVHYA----CPSCGASESSYLFAAKFGKYVEWVTRDLVKFDLKQKKDRVRLNKFGGRI 56

Query: 2527 SYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFLEH 2348
            S +HHV+KALATSDVSVV+ +  +A+YRF  EIGGQLKV ++K  N+K+ +HVE   L  
Sbjct: 57   SLEHHVMKALATSDVSVVQTTGKLASYRFRTEIGGQLKVLVRK--NSKFEVHVEASNLPG 114

Query: 2347 CEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDESL 2168
             E +G L M WGLFRSDS+ FMPL+F+QS+ADSK + +ETPF K+SVG LAVELDFD SL
Sbjct: 115  EEDEG-LIMRWGLFRSDSSIFMPLNFQQSTADSKDNVVETPFLKDSVGRLAVELDFDASL 173

Query: 2167 APFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAFFS 1988
             PFYVS LLKS   SN K+ TIRSHR TNF+VPVGFGSGRP PLG+SF DD  INFAFFS
Sbjct: 174  TPFYVSFLLKS---SNSKIPTIRSHRTTNFVVPVGFGSGRPFPLGLSFLDDRSINFAFFS 230

Query: 1987 RNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTENK 1808
             N E VVLCLY+D  ADKPALEIDLDPYVNRSG IWHAL+  SLPFV YGYRCR G  N+
Sbjct: 231  SNAEGVVLCLYSDGTADKPALEIDLDPYVNRSGYIWHALIQDSLPFVSYGYRCRGGIANR 290

Query: 1807 GHRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFT 1628
            G  VLLDPYA VI+ F L L RK+LGKLCEEPAFDWSG+ RPSLP+E+LI+YRLNVTRFT
Sbjct: 291  GQHVLLDPYATVIKSFGLGLNRKWLGKLCEEPAFDWSGESRPSLPLEKLIVYRLNVTRFT 350

Query: 1627 IDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLY 1448
             DKSSKLP   AGTF GI EKLHHFK+LGVNA+LLEPIFP DE+K PYFPWHFFSPGS Y
Sbjct: 351  KDKSSKLPTSAAGTFYGIYEKLHHFKDLGVNAILLEPIFPSDERKEPYFPWHFFSPGSSY 410

Query: 1447 GPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLK 1268
            GPS D   + KSMKEMVKKLHASGIEVLLEVVFTHT E  +L+ ID S Y+S+   EDLK
Sbjct: 411  GPSDDLSYIVKSMKEMVKKLHASGIEVLLEVVFTHTGEDVTLKGIDESYYHSI---EDLK 467

Query: 1267 SRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAF 1088
            S+N LNCNYP+VQQ+ILESLR+WVIE+HIDGFCF+NASSL RG HGEFLSRPPL+EAIAF
Sbjct: 468  SKNMLNCNYPVVQQLILESLRHWVIEYHIDGFCFINASSLLRGKHGEFLSRPPLVEAIAF 527

Query: 1087 DPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRL 908
            DPLLS++K+IADSWDPH +E+KE  FPHWKRWAEMNGKFC D RNFLRGQG IS+L TR 
Sbjct: 528  DPLLSQIKIIADSWDPHGMESKELVFPHWKRWAEMNGKFCADARNFLRGQGSISSLTTRF 587

Query: 907  CGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLE 728
            CGSGD+F  GRGPAFSFN+ITRNVGLTL+DLVSF   ++  Q SWNCGEEGPTNK  VLE
Sbjct: 588  CGSGDIFAGGRGPAFSFNYITRNVGLTLIDLVSFGSEEIASQLSWNCGEEGPTNKISVLE 647

Query: 727  TRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQF 548
             RLKQIRNFLF+LF+SLGVPVLNMGDE GQS  GS  YTDR PLDWN L+SGF IQ+TQF
Sbjct: 648  RRLKQIRNFLFLLFLSLGVPVLNMGDEFGQSCAGSLAYTDRTPLDWNSLRSGFSIQITQF 707

Query: 547  ISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQST 368
            ISFL SLK+RR D  Q +SFL+E+ IEW GT+  PPKWDD S KF+++TLK   + SQST
Sbjct: 708  ISFLCSLKMRRGDFFQRKSFLEEDKIEWHGTDLSPPKWDDPSTKFVAVTLKVGNKLSQST 767

Query: 367  SGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLA 188
            S      GDLF+AFNS D SE+V LPPL  +  WV LVDTAL FPGFF+VDGVPLE GL 
Sbjct: 768  SAFPEPSGDLFVAFNSDDQSEEVALPPLPDNTAWVRLVDTALTFPGFFSVDGVPLEDGLT 827

Query: 187  TYEMKSHSCILFEARSL 137
            TYEM+SHSC+LFEAR+L
Sbjct: 828  TYEMQSHSCVLFEARNL 844


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score = 1179 bits (3050), Expect = 0.0
 Identities = 586/769 (76%), Positives = 653/769 (84%), Gaps = 3/769 (0%)
 Frame = -1

Query: 2437 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2258
            MEIGGQLKV +KK +NNKYG++VEVE L+  E Q EL MIWG+FR D T FMP+  +QSS
Sbjct: 1    MEIGGQLKVMVKKNNNNKYGVYVEVESLQLDEEQRELIMIWGIFRDDLTKFMPV--QQSS 58

Query: 2257 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2078
             D    T ETPF K+    L+VEL+F+ESLAPFYVSILLKS+LGSN K STIRSHRKTNF
Sbjct: 59   TDG---TTETPFVKDC-DRLSVELEFEESLAPFYVSILLKSKLGSNSKTSTIRSHRKTNF 114

Query: 2077 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1898
            IVPVGF SGRPSPLGIS  +DG INFAFFSRN  SVVLCLYADT  DKPALEIDLDPYVN
Sbjct: 115  IVPVGFRSGRPSPLGISLLNDGFINFAFFSRNAYSVVLCLYADTTVDKPALEIDLDPYVN 174

Query: 1897 RSGDIWHALVDCS-LPFVGYGYRCRNG-TENKGHRVLLDPYARVIEDFWLDLPRKFLGKL 1724
            RSGDIWHA +DCS LPFV YGYRCR+  T++KGHRVL DPYA+VIE+F    PRK LG+L
Sbjct: 175  RSGDIWHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRL 231

Query: 1723 CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 1544
            C E AFDWSG+ RP+LPME+LI+YRLNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNL
Sbjct: 232  CNESAFDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNL 291

Query: 1543 GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 1364
            GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVL
Sbjct: 292  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVL 351

Query: 1363 LEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 1184
            LEVVFTHTSE+++LREIDNSSY+ V GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFH
Sbjct: 352  LEVVFTHTSEISALREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFH 411

Query: 1183 IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPH 1004
            IDGFCF+N+SSLTRG HGEFLSRPPL+EAIAFDPLLSKVK++ADSWDPH +E KE  FPH
Sbjct: 412  IDGFCFINSSSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPH 471

Query: 1003 WKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTL 824
            WKRWAEMN KFC ++RNF RG+G IS+LATRLCGSGDLFL GR PAFSFNF+TRNVG TL
Sbjct: 472  WKRWAEMNAKFCSEMRNFFRGKGSISSLATRLCGSGDLFLGGRSPAFSFNFVTRNVGPTL 531

Query: 823  VDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDEC 644
            VDLVS++      + SWNCGEEGPTNK +VLETRLKQIRNF+FVLF+SLGVPVLNMGDEC
Sbjct: 532  VDLVSYNSI----ESSWNCGEEGPTNKILVLETRLKQIRNFIFVLFVSLGVPVLNMGDEC 587

Query: 643  GQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEW 464
            GQST GSP Y+DR   DW+ LKSGFG QVT+FISFLSSL++RR+DLLQ RSFLKE+NIEW
Sbjct: 588  GQSTRGSPEYSDRISFDWSALKSGFGTQVTEFISFLSSLRIRRNDLLQKRSFLKEDNIEW 647

Query: 463  RGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPL 284
             G E  PP WDD SCKFL+MTLK D   S           DLF+AFN GD SEKV LPPL
Sbjct: 648  HGAEGGPPMWDDPSCKFLAMTLKADPPLS-----------DLFVAFNGGDCSEKVALPPL 696

Query: 283  -AVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARS 140
             AVD  WVCLVDT+LPFPGFFT +GVPLE GL TYEMKSHSC+LFEARS
Sbjct: 697  AAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLETYEMKSHSCVLFEARS 745


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score = 1125 bits (2910), Expect = 0.0
 Identities = 551/867 (63%), Positives = 668/867 (77%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA LP+ + +QSHC+   A ESSK   A+     +RV  +  + + K+  VHG++ K A 
Sbjct: 1    MATLPLSFTIQSHCVYCGATESSKSVVATHFNYRKRVAQNLQQ-SYKRNFVHGEVKKYAT 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
            + S+ H  LK LATS +SV +  E  + Y F  EIGG +KV +++K N KY ++VEV  L
Sbjct: 60   KDSFQHRNLKVLATSRISVGQKEEKSSAYLFRTEIGGHVKVLVRQK-NLKYSVYVEVSSL 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            +   G+ +L M WG+FRSDS+ F+PLD + S+ D + ST+ETPF +NS+G L +EL+F+ 
Sbjct: 119  KLKSGEDKLVMNWGIFRSDSSQFIPLDLQSSTPDDRSSTVETPFVQNSLGKLVIELNFEG 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SL PFY+S LLK     + K S +RSHRKT+F VPVG  SG P+PLG+SF+ DG INFA 
Sbjct: 179  SLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSFCVPVGLASGYPAPLGLSFSADGSINFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FS++ + VVLCLY DT+A+KP LEIDLDPYVNRSGDIWH  +D +LPFV YGYRC+ G  
Sbjct: 239  FSQSADGVVLCLYDDTKAEKPTLEIDLDPYVNRSGDIWHVSMDSALPFVSYGYRCKGGIH 298

Query: 1813 NKG-----HRVLLDPYARVIEDFWLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIYR 1649
             KG       VLLDPYA+VI +       K LGKL +EP FDWSGD RP +PME+L++YR
Sbjct: 299  GKGVKDHSEHVLLDPYAKVIANAHSGSVLKTLGKLIKEPTFDWSGDIRPCIPMEKLVVYR 358

Query: 1648 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWHF 1469
            +NV  FT  KSS LP D+AG+F GI+EKL H K+LGVNA+LLEP+FPFDE+KGPYFP+HF
Sbjct: 359  VNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLGVNAILLEPVFPFDEKKGPYFPFHF 418

Query: 1468 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 1289
            FSP  LYGPS DP     SMKEMVK LHA+GIEVLLE+VFTHT+E  SLREID SSYY +
Sbjct: 419  FSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLLEIVFTHTAESCSLREIDKSSYY-I 477

Query: 1288 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRPP 1109
             G EDL ++NALNCNYPIVQQMIL+SLRYW IEFH+DGFCF+NASSL +G HGEFLSRPP
Sbjct: 478  TGDEDLGAKNALNCNYPIVQQMILDSLRYWAIEFHVDGFCFINASSLLKGFHGEFLSRPP 537

Query: 1108 LIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGPI 929
            L+EAIAFDPLLSK KLIADSWDPH + + E  FPHWKRWAE+N  FC DVRNFLRG+G +
Sbjct: 538  LVEAIAFDPLLSKTKLIADSWDPHDMASDEILFPHWKRWAEVNTNFCGDVRNFLRGEGLL 597

Query: 928  SNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPT 749
            SNLATRLCGSGD+FL+GRGP FSFN+ITRN GL LVDLVSFS S+L  + SWNCGEEGPT
Sbjct: 598  SNLATRLCGSGDMFLNGRGPGFSFNYITRNSGLPLVDLVSFSSSNLASELSWNCGEEGPT 657

Query: 748  NKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSGF 569
            N T VLE RLKQIRN+LF+LF+S GVPVLNMGDECGQS+GGSP Y DRKP DWN L++GF
Sbjct: 658  NNTAVLERRLKQIRNYLFILFVSFGVPVLNMGDECGQSSGGSPAYGDRKPYDWNALRTGF 717

Query: 568  GIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKTD 389
            GIQ TQFISFLSSL+VRRSDLLQ RSFLKEENI+W G++Q  P WDD S +FL+MTLK +
Sbjct: 718  GIQTTQFISFLSSLRVRRSDLLQKRSFLKEENIDWYGSDQSLPTWDDRSSRFLAMTLKVE 777

Query: 388  AQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDGV 209
             + ++S+S   +  GDLF+AFNS D SE+V LPP   +  W+ LVDTA+PFPGFF+ DG 
Sbjct: 778  REENESSSEFSSLNGDLFVAFNSADQSERVILPPPPPEMVWLRLVDTAVPFPGFFSRDGE 837

Query: 208  PL---EAGLATYEMKSHSCILFEARSL 137
            P+    AGL TYEMKSHSC+LFEA+ L
Sbjct: 838  PVLEQMAGLVTYEMKSHSCVLFEAKKL 864


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score = 1097 bits (2837), Expect = 0.0
 Identities = 546/867 (62%), Positives = 662/867 (76%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA L V   +QS  LS    ES+KL  A    +G+RV     KL L++    G+  K  +
Sbjct: 1    MATLTVQLAVQSRLLSYGIGESTKLVPAPPANHGKRVLCRLRKLELEEINFSGRSRKTDQ 60

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
             +S   H LKAL+TS +S+++ ++ V TY F  + GGQ+KV ++K +N KY + VE    
Sbjct: 61   EVSKRTHRLKALSTSGISLIQTAKTVPTYLFRTDTGGQVKVLVEK-TNGKYKVLVEALPS 119

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL M+WGLFRSD++CFMPLD  +  AD K STIETPF +     + VELDF+ 
Sbjct: 120  ELSDAHAELVMVWGLFRSDASCFMPLDLNRRGADGKSSTIETPFVQGPSNKVTVELDFEA 179

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S++K S IRSHR T+F+VPVG  SG P+PLG+S+  DG +NFA 
Sbjct: 180  SLAPFYISFYMKSQLVSDMKSSEIRSHRNTSFVVPVGLTSGHPAPLGLSYQPDGTMNFAL 239

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+ +SVVLCLY D   +KP+LEIDLDPYVNRSGDIWHA +DCSLPF  YGYRC+  T 
Sbjct: 240  FSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNRSGDIWHAALDCSLPFKTYGYRCKVATS 299

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             K   VLLDPY++VI      +PR        K+LG+LC+EP +DWSGD  PSLP+E LI
Sbjct: 300  GKEEMVLLDPYSKVIRSV---IPRQGGSKLRPKYLGELCKEPGYDWSGDVPPSLPLENLI 356

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYRLNVT FT D SSKLP D+AGTF+GI+EKL HFK+LGVNA+LLEPIFPF+EQKGPYFP
Sbjct: 357  IYRLNVTEFTKDGSSKLPDDLAGTFSGITEKLSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
             HFFSPG+++G S DP +V  SMK+MVKKLHA+GIE+ LEVVFTH +E A L  +DNSS+
Sbjct: 417  SHFFSPGNMHGRSSDPLSVINSMKDMVKKLHANGIEIFLEVVFTHIAEDAPLMHVDNSSH 476

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
            YS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF FVNASSL++G HGE LS
Sbjct: 477  YSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLSKGFHGEILS 535

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  FPHW++WAE+N +FC D+R+FLRG+
Sbjct: 536  RPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            GP    +VLETRLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GG PV+  RK  DW+ LK
Sbjct: 656  GPMTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGFPVHGARKSFDWSTLK 715

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI+W G++Q PPKWDD S +FL+MTL
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMTL 775

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ TS S N GGDLF+AFNS   SE V LPP   D  W  LVDT LPFPGFF  
Sbjct: 776  KADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVILPPPPTDMVWHRLVDTGLPFPGFFAE 833

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Solanum pennellii]
          Length = 866

 Score = 1097 bits (2837), Expect = 0.0
 Identities = 544/867 (62%), Positives = 656/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            M   P+   + SH LS  + ES+KL  AS G  G+ V S   KL L+     G      +
Sbjct: 1    METSPIQLAVHSHLLSYGSTESTKLVPASSGNRGKIVCSP-RKLELEDMNFSGIGRNNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
                  H LKAL+ S +S+V  ++ V TY F   IGGQ+KVF++K +N KY + VEV  L
Sbjct: 60   EAPRRAHRLKALSGSRISLVPSAKRVPTYLFRTAIGGQVKVFVEK-TNGKYKVLVEVLPL 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL M+WGLFRSD++CFMPLD  +  AD K ST+ETPF +   G + VELDF+ 
Sbjct: 119  ELSDAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEV 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SL PFY+S  +KSQL S+++ S IRSHR TNF+VPVG  SG P+PLGISF  DG +NFA 
Sbjct: 179  SLVPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGYVNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            +SR+ + VVLCLY D   +KP+LEIDLDPY+NRSGDIWHA +DCSLPF  YGYR +  T 
Sbjct: 239  YSRSAKGVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRFKAATS 298

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             KG  VLLDPYA+VI      +PR        K+LG+LC EP +DWSGD  PSLPME+LI
Sbjct: 299  GKGELVLLDPYAKVIRSV---IPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYR+NVT FT DKSSKLP ++AGTF+GISEK HH K+LGVNA+LLEPIFPFDEQKGPY+P
Sbjct: 356  IYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYP 415

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
            WHFFSPG++YGPSGDP +V K+MK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSVIKTMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
              + GG+DL  +NALNCN PIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE LS
Sbjct: 476  -CIKGGQDLNIQNALNCNCPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILS 534

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFD +LSKVK+IAD+W+P + ++ E  FPHW+RWAE+N +FC D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDLILSKVKMIADNWNPLTNDSTENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +SNLATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSNSEVASELSWNCGQE 654

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            G T  ++VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK LDWN LK
Sbjct: 655  GATTNSIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYDARKSLDWNTLK 714

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEENI+W G++Q PPKWDD S KFL+MTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEENIQWHGSDQSPPKWDDPSSKFLAMTL 774

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ S +    +  GGDLF+AFN    SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 775  KADAEVSHTLLSDI--GGDLFVAFNGAGDSESVILPPPPTDMVWYRLVDTALPFPGFFDE 832

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCVLFEAQRL 859


>ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
 ref|XP_015168040.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
          Length = 878

 Score = 1097 bits (2837), Expect = 0.0
 Identities = 547/867 (63%), Positives = 656/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA  P+   + S  LS  + ES+KL  +S G  G+ V S   KL L+     G      +
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVCS-LRKLELEDMNFSGIGRNNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
                  H  KAL+ S +S+V  ++ V TY F  +IGGQ+KV +++ +N KY + VEV  L
Sbjct: 60   EAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVER-TNGKYKVLVEVLPL 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E      EL M+WGLFRSD++CFMPLD  +  AD K ST+ETPF +   G + VELDF+ 
Sbjct: 119  ELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEA 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S+++ S IRSHR TNF+VPVG  SG P+PLGISF  DG +NFA 
Sbjct: 179  SLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+  SVVLCLY D   +KP+LEIDLDPY+NRSGDIWHA +DCSLPF  YGYRC+  T 
Sbjct: 239  FSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKATTS 298

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             KG  VLLDPYA+VI      +PR        K+LG+LC EP +DWSGD  PSLPME+LI
Sbjct: 299  GKGELVLLDPYAKVIRRV---IPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYRLNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 356  IYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFP 415

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
            WHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
              + GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE LS
Sbjct: 476  -CIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILS 534

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSKVK+IAD+W+P + ++KE  FPHW+RWAE+N +FC D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +SNLATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS +++  + SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQE 654

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            G T   +VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK L WN LK
Sbjct: 655  GATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYDARKSLGWNTLK 714

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEENI+W G++Q PPKWD  S KFL+MTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTL 774

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ+    +   GDLF+AFN    SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 775  KADAEVSQTLVSDI--VGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDE 832

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum]
 gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score = 1095 bits (2832), Expect = 0.0
 Identities = 546/867 (62%), Positives = 655/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA  P+   + S  LS  + ES+KL  +S G  G+ V S   KL L+     G      +
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVCS-LRKLELEDMNFSGIGRNNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
                  H  KAL+ S +S+V  ++ V TY F  +IGGQ+KV +++ +N KY + VEV  L
Sbjct: 60   EAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVER-TNGKYKVLVEVLPL 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E      EL M+WGLFRSD++CFMPLD  +  AD K ST+ETPF +   G + VELDF+ 
Sbjct: 119  ELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEA 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S+++ S IRSHR TNF+VPVG  SG P+PLGISF  DG +NFA 
Sbjct: 179  SLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+  SVVLCLY D   +KP+LEIDLDPY+NRSGDIWHA +DCSLPF  YGYRC+  T 
Sbjct: 239  FSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKATTS 298

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             KG  VLLDPYA+VI      +PR        K+LG+LC EP +DWSGD  PSLPME+LI
Sbjct: 299  GKGELVLLDPYAKVIRRV---IPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYRLNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 356  IYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFP 415

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
            WHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
              + GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE LS
Sbjct: 476  -CIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILS 534

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSKVK+IAD+W+P + ++KE  FPHW+RWAE+N +FC D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +SNLATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS +++  + SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQE 654

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            G T   +VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GG P Y  RK L WN LK
Sbjct: 655  GATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNTLK 714

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEENI+W G++Q PPKWD  S KFL+MTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTL 774

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ+    +   GDLF+AFN    SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 775  KADAEVSQTLVSDI--VGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDE 832

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score = 1091 bits (2822), Expect = 0.0
 Identities = 546/867 (62%), Positives = 659/867 (76%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA   V   +QS  LS    ES++L  A     G+RV     KL L++    G+  K  +
Sbjct: 1    MATSTVQLAVQSRLLSYGIGESTELVPAPPANRGKRVVCRLRKLELEEMNFSGRSRKIDQ 60

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
             +S   H LKAL+ S +S+++ ++ V TY F  +IGGQ+KV ++K +N KY + VEV   
Sbjct: 61   EVSKRSHRLKALSASGISLIQTAKRVPTYLFRTDIGGQVKVLVEK-TNGKYKVLVEVLPS 119

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL M+WGLFRSD++CFMPLD  +  AD K STIETPF +     + VELDF+ 
Sbjct: 120  ELSDAHAELVMVWGLFRSDASCFMPLDLNRHGADGKSSTIETPFVQGPSDKVTVELDFEA 179

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S++K S IRSHR T F+VPVG  SG P+PLG+S   DG +NFA 
Sbjct: 180  SLAPFYISFYMKSQLVSDMKSSEIRSHRNTTFVVPVGLTSGHPAPLGLSCQPDGTMNFAL 239

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+ +SVVLCLY D   +KP+LEIDLDPYVNRSGDIWHA +DCSLP   YGYRC+  T 
Sbjct: 240  FSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNRSGDIWHAALDCSLPIKTYGYRCKVATS 299

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             K   VLLDPY++VI      +PR        K+LG+LC+EP +DWSGD  PSL ME+LI
Sbjct: 300  GKEELVLLDPYSKVIRSV---IPRQGGSKLRPKYLGELCKEPGYDWSGDVPPSLHMEKLI 356

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYRLNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNA+LLEPIFPF+EQKGPYFP
Sbjct: 357  IYRLNVTEFTNDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
             HFFSPG+++G SGDP +V KSMK+MVKKLHA+GIE+ LEVVFTHT+E A L  +DNSSY
Sbjct: 417  SHFFSPGNMHGHSGDPLSVIKSMKDMVKKLHANGIEIFLEVVFTHTAEDAPLMHVDNSSY 476

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
            YS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF F+NASSL +G HGE LS
Sbjct: 477  YSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFINASSLLKGFHGEILS 535

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  FPHW++WAE+N +FC D+R+FLRG+
Sbjct: 536  RPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINKRFCDDIRDFLRGK 595

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +S LATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEE
Sbjct: 596  GLLSGLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            GPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGDECGQS+ GSPV+  RK  DW+ LK
Sbjct: 656  GPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSRGSPVHGARKSFDWSTLK 715

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFGIQ TQFISFL+SL++RRSDLLQ R+FL+EENI+W G++Q PPKWDD S +FL+MTL
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLEEENIQWHGSDQSPPKWDDPSSRFLAMTL 775

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ TS S N GGDLF+AFNS   SE V LPP   D  W  LVDTALPFP FF  
Sbjct: 776  KADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVILPPPPTDMVWHRLVDTALPFPEFFAE 833

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
 ref|XP_009761455.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
          Length = 863

 Score = 1090 bits (2818), Expect = 0.0
 Identities = 543/867 (62%), Positives = 658/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA   V   +QS  LS    ES+KL  A     G+RV    +KL L +    G+  K  +
Sbjct: 1    MATSTVQLALQSRLLSYGIGESTKLVPAPTANRGKRVACRLSKLELGEMNFSGRSRKTDQ 60

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
             +S   H LKAL+ S +S+++ ++ V TY F  +IGG +KV ++K +N KY + VEV   
Sbjct: 61   EVSKRTHRLKALSASGISLIQTAKRVPTYLFRTDIGGHVKVLVEK-TNGKYIVLVEVLPS 119

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            EH +   EL M+WGLFRSD++CFMPLD  +  AD + ST+ETPF +     + VELDF+ 
Sbjct: 120  EHSDAHAELVMLWGLFRSDASCFMPLDLNRRGADGQSSTLETPFVQGPSDKVTVELDFEA 179

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S++K S IRSHR TNF+VPVG  SG PSPLG+S   DG +NFA 
Sbjct: 180  SLAPFYISFYMKSQLVSDMKRSEIRSHRNTNFVVPVGLTSGHPSPLGLSCQPDGTMNFAL 239

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+ +SVVLCLY D   + P+LEIDLDPYVNRSGDIWHA +DCSL F  YGYRC+  T 
Sbjct: 240  FSRSAKSVVLCLYDDISVEMPSLEIDLDPYVNRSGDIWHAALDCSLSFKTYGYRCKVATS 299

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             K   VLLDPY++VI      +PR        K+LG+LC+EP +DWS D  PSLPME+LI
Sbjct: 300  GKEELVLLDPYSKVIRSV---IPRQGGSKLHPKYLGELCKEPGYDWSDDVPPSLPMEKLI 356

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            +YRLNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNA+LLEPIFPF+EQKGPYFP
Sbjct: 357  VYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
             HFFSPG+++G S DP +V KSMK+MVKKLHA+GIE+ LEV+FTHT+E A L  IDNS Y
Sbjct: 417  SHFFSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIEIFLEVIFTHTAEDAPLMHIDNSCY 476

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
            YS+ GG D+  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF FVNASSL +G HGE LS
Sbjct: 477  YSIKGG-DMNIQFALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLLKGFHGEILS 535

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RP L+EAIAFDP+LSKVK+IAD WD  + ++KE  FPHW++WAE+N +FC D+R+FLRG+
Sbjct: 536  RPQLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            GPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSPV+  RK  DW+ LK
Sbjct: 656  GPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPVHGARKSFDWSTLK 715

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI+W G++Q PPKWDD S +FL+M+L
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMSL 775

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ TS S N GGDLF+AFNS   SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 776  KADAEGSQ-TSVSDN-GGDLFVAFNSAGESESVILPPPPTDVVWHRLVDTALPFPGFFAE 833

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E GL  YEMK HSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKPHSCLLFEAQKL 860


>ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
 ref|XP_016475508.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score = 1088 bits (2813), Expect = 0.0
 Identities = 542/867 (62%), Positives = 657/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA   V   +QS  LS    ES+KL  A     G+RV    +KL L +    G+  K  +
Sbjct: 1    MATSTVQLALQSRLLSYGIGESTKLVPAPTANRGKRVACRLSKLELGEMNFSGRSRKTDQ 60

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
             +S   H LKAL  S +S+++ ++ V TY F  +IGG +KV ++K +N KY + VEV   
Sbjct: 61   EVSKRTHRLKALPASGISLIQTAKRVPTYLFRTDIGGHVKVLVEK-TNGKYIVLVEVLPS 119

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            EH +   EL M+WGLFRSD++CFMPLD  +  AD + ST+ETPF +     + VELDF+ 
Sbjct: 120  EHSDAHAELVMLWGLFRSDASCFMPLDLNRRGADGQSSTLETPFVQGPSDKVTVELDFEA 179

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S++K S IRSHR TNF+VPVG  SG PSPLG+S   DG +NFA 
Sbjct: 180  SLAPFYISFYMKSQLVSDMKRSEIRSHRNTNFVVPVGLTSGHPSPLGLSCQPDGTMNFAL 239

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+ +SVVLCLY D   + P+LEIDLDPYVNRSGDIWHA +DCSL F  YGYRC+  T 
Sbjct: 240  FSRSAKSVVLCLYDDISVEMPSLEIDLDPYVNRSGDIWHAALDCSLSFKTYGYRCKVATS 299

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             K   VLLDPY++VI      +PR        K+LG+LC+EP +DWS D  PSLPME+LI
Sbjct: 300  GKEELVLLDPYSKVIRSV---IPRQGGSKLHPKYLGELCKEPGYDWSDDVPPSLPMEKLI 356

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            +YRLNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNA+LLEPIFPF+EQKGPYFP
Sbjct: 357  VYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
             HFFSPG+++G S DP +V KSMK+MVKKLHA+GIE+ LEV+FTHT+E A L  IDNS Y
Sbjct: 417  SHFFSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIEIFLEVIFTHTAEDAPLMHIDNSCY 476

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
            YS+ GG D+  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF FVNASSL +G HGE LS
Sbjct: 477  YSIKGG-DMNIQFALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLLKGFHGEILS 535

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RP L+EAIAFDP+LSKVK+IAD WD  + ++KE  FPHW++WAE+N +FC D+R+FLRG+
Sbjct: 536  RPQLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            GPT   +VLETR+KQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSPV+  RK  DW+ LK
Sbjct: 656  GPTTNNIVLETRVKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPVHGARKSFDWSTLK 715

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI+W G++Q PPKWDD S +FL+M+L
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMSL 775

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ TS S N GGDLF+AFNS   SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 776  KADAEGSQ-TSVSDN-GGDLFVAFNSAGESESVILPPPPTDVVWHRLVDTALPFPGFFAE 833

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E GL  YEMK HSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKPHSCLLFEAQKL 860


>ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Solanum lycopersicum]
          Length = 866

 Score = 1088 bits (2813), Expect = 0.0
 Identities = 538/867 (62%), Positives = 654/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            M   P+   + S  LS  + ES+KL  AS G  G+ V S   KL L+     G      +
Sbjct: 1    METSPIQLAVHSRLLSYGSTESTKLVPASSGNRGKIVCS-LRKLELEDMNFSGISRNNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
                  H LKAL+ S +S+V  ++ V TY F  +IG Q+KVF++K +N KY + VEV  L
Sbjct: 60   EAPRRAHRLKALSGSRISLVPSAKRVPTYLFRTDIGCQVKVFVEK-TNGKYKVLVEVLPL 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL M+WGLFRSD++CFMPLD  +  AD K ST+ETPF +   G + VELDF+ 
Sbjct: 119  ELSDAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEV 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SL PFY+S  +KSQL S+++ S IRSHR TNF+VPVG  SG P+PLGISF  DG +NFA 
Sbjct: 179  SLVPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGYVNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            +SR+ + VVLCLY D   +KP+LEIDLDPY+N+SGDIWHA +DCSLPF  YGYR +  T 
Sbjct: 239  YSRSAKGVVLCLYDDISVEKPSLEIDLDPYINQSGDIWHAALDCSLPFKTYGYRFKAATS 298

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             KG  VLLDPYA+VI      +PR        K+LG+LC EP +DWSGD  PSLPME+LI
Sbjct: 299  GKGELVLLDPYAKVIRSV---IPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYR+NVT FT DKSSKLP ++AGTF+GISEK HH K+LGVNA+LLEPIFPFDEQKGPY+P
Sbjct: 356  IYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYP 415

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
            WHFFSPG++YGPSGDP +V KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
              + G +D+  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FV+ASSL RG +GE LS
Sbjct: 476  -CIKGSQDMNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVDASSLLRGFNGEILS 534

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSK K+IAD+W+P + ++ E  FPHW+RWAE+N +FC D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKAKMIADNWNPLTNDSTENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +SNLATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSNSEVASELSWNCGQE 654

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            G T  ++VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK L+WN LK
Sbjct: 655  GATTNSIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYDARKSLNWNTLK 714

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEENI+W G++Q PP WDD S KFL+MTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEENIQWHGSDQSPPVWDDPSSKFLAMTL 774

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ S +    +  GGDLF+AFN    SE V LPP   D  W  LVDTALPFPGFF  
Sbjct: 775  KADAEVSHTLLSDI--GGDLFVAFNGAGDSESVILPPPPTDMVWYRLVDTALPFPGFFDE 832

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCVLFEAQRL 859


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
 ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score = 1087 bits (2811), Expect = 0.0
 Identities = 545/867 (62%), Positives = 658/867 (75%), Gaps = 8/867 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA   V   +QS  LS    ES++L  A     G+RV     KL L++    G+  K  +
Sbjct: 1    MATSTVQLAVQSRLLSYGIGESTELVPAPPANRGKRVVCRLRKLELEEMNFSGRSRKIDQ 60

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
             +S   H LKAL+ S +S+++ ++ V TY F  +IGGQ+KV ++K +N KY + VEV   
Sbjct: 61   EVSKRTHRLKALSASGISLIQTAKRVPTYLFRTDIGGQVKVLVEK-TNGKYKVLVEVLPS 119

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL M+WGLFRSD++CFMPLD  +  AD K STIETPF +     + VELDF+ 
Sbjct: 120  ELSDAHAELVMVWGLFRSDASCFMPLDLNRHGADGKSSTIETPFVQGPSDKVTVELDFEA 179

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAPFY+S  +KSQL S++K S IRSHR T F+VPVG  SG P+PLG+S   DG +NFA 
Sbjct: 180  SLAPFYISFYMKSQLVSDMKSSEIRSHRNTTFVVPVGLTSGHPAPLGLSCQPDGTMNFAL 239

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FSR+ +SVVLCLY D   +KP+LEIDLDPYVNRSGDIWHA +DCSLP   YGYRC+  T 
Sbjct: 240  FSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNRSGDIWHAALDCSLPIKTYGYRCKVATS 299

Query: 1813 NKGHRVLLDPYARVIEDFWLDLPR--------KFLGKLCEEPAFDWSGDFRPSLPMERLI 1658
             K   VLLDPY++VI      +PR        K+LG+LC+EP +DWS D  PSL ME+LI
Sbjct: 300  GKEELVLLDPYSKVIRSV---IPRQGGSKLRPKYLGELCKEPGYDWSVDVPPSLHMEKLI 356

Query: 1657 IYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFP 1478
            IYRLNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNA+LLEPIFPF+EQKGPYFP
Sbjct: 357  IYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1477 WHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSY 1298
             HFFSPG+++G SGDP +V KSMK+MVKKLHA+GIE+ LEVVFTHT+E A L  +DNSSY
Sbjct: 417  SHFFSPGNMHGRSGDPLSVIKSMKDMVKKLHANGIEIFLEVVFTHTAEDAPLMHVDNSSY 476

Query: 1297 YSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLS 1118
            YS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF F+NASSL +G HGE  S
Sbjct: 477  YSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFINASSLLKGFHGEIQS 535

Query: 1117 RPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQ 938
            RPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  FPHW++WAE+N +FC D+R+FLRG+
Sbjct: 536  RPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 937  GPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEE 758
            G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 757  GPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLK 578
            GPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGDECGQS+ GSPV+  RK  DW+ LK
Sbjct: 656  GPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSRGSPVHGARKSFDWSTLK 715

Query: 577  SGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTL 398
            +GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI+W G++Q PPKWDD S +FL+MTL
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMTL 775

Query: 397  KTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTV 218
            K DA+ SQ TS S N GGDLF+AFNS   SE V LPP   D  W  LVDTALPFP FF  
Sbjct: 776  KADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVILPPPPTDMVWHRLVDTALPFPEFFAE 833

Query: 217  DGVPLEAGLATYEMKSHSCILFEARSL 137
             G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]
          Length = 862

 Score = 1086 bits (2809), Expect = 0.0
 Identities = 544/865 (62%), Positives = 660/865 (76%), Gaps = 6/865 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA  P+   + S  LS  + ES+KL  A  G  G+ V S   KL L++    G   K  +
Sbjct: 1    MATSPIQLAVHSRLLSYGSSESTKLVPAPPGNRGKIVCS-VKKLELEEMNFSGIGRKNYQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
            ++S   H LKAL+ + +S+V   + V TY F  +IGGQ+KV+++K +N KY + VEV  +
Sbjct: 60   QVSGRFHQLKALSATGISLVPTVKKVPTYLFRTDIGGQVKVYVEK-TNGKYKVLVEVLPV 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL ++ G+FRSD +CFMPLD  +  AD K STIETPF +   G + VELDF+ 
Sbjct: 119  ELSDAHAELVIVCGVFRSDVSCFMPLDLNRCGADGKSSTIETPFVQGPSGKVTVELDFEA 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAP Y+S  +KSQL ++++ S IRSHR TNF+VPVGF SG PSPLG+SF  DG +NFA 
Sbjct: 179  SLAPCYISFYIKSQLVTDMESSEIRSHRNTNFVVPVGFSSGHPSPLGLSFLPDGSLNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FS + +SVVLCLY DT A+KP LEIDLDPYVNRSGDIWHA +DCSL F  YGYRC+  T 
Sbjct: 239  FSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVNRSGDIWHAALDCSLLFKTYGYRCKVATS 298

Query: 1813 NK-GHRVLLDPYARVIEDF-----WLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIY 1652
             K G  VLLDPYA+VI          +   K+LG+LC+EP +DWSGD RPSLPME+LIIY
Sbjct: 299  GKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGELCQEPGYDWSGDVRPSLPMEKLIIY 358

Query: 1651 RLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWH 1472
            RLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+LGVNA+LL+PIFPF+EQKGPY+P H
Sbjct: 359  RLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDLGVNAMLLQPIFPFEEQKGPYYPCH 418

Query: 1471 FFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYS 1292
            FFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV LEVVFTHT++ A L  +DN+SYY 
Sbjct: 419  FFSPGNMYGRSGDALSVIKSVKDMVKKLHANGIEVFLEVVFTHTAQDAPLMHVDNTSYY- 477

Query: 1291 VNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRP 1112
            + GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF IDGF F+NASSL RG HGE LSRP
Sbjct: 478  IKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFRIDGFVFINASSLLRGFHGEILSRP 536

Query: 1111 PLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGP 932
            PL+EAIAFDP+LS VK+IAD+W+P + ++KE  FPHW+RWAE+N +FC D+R+FLRG+G 
Sbjct: 537  PLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPHWRRWAEINLRFCADIRDFLRGEGL 596

Query: 931  ISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGP 752
            +SNLATRLCGSGD+F  GRGPAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG 
Sbjct: 597  LSNLATRLCGSGDIFSGGRGPAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGA 656

Query: 751  TNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSG 572
            T  T+VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK  DWN LK+G
Sbjct: 657  TTNTIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTG 716

Query: 571  FGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKT 392
            FGIQ TQFISFLS+L++RRSDLLQ  +FLKEENI W G +Q PPKWDD S KFL+MTLK 
Sbjct: 717  FGIQTTQFISFLSNLRMRRSDLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKA 776

Query: 391  DAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDG 212
            DA+ SQ+    +  GGDLF+AFNS   SE V LPP   D  W  LVDTALPFPGFF   G
Sbjct: 777  DAEVSQTPVSEI--GGDLFVAFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKG 834

Query: 211  VPLEAGLATYEMKSHSCILFEARSL 137
             P+E  L  YEMKSHSC+LFEA  L
Sbjct: 835  TPVEDELVAYEMKSHSCLLFEAHRL 859


>gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]
          Length = 862

 Score = 1083 bits (2801), Expect = 0.0
 Identities = 543/865 (62%), Positives = 660/865 (76%), Gaps = 6/865 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA  P+   + S  LS  + ES+KL  A  G  G+ V S   K  L++    G   K  +
Sbjct: 1    MATSPIQLAVHSRLLSYGSSESTKLVPAPPGNRGKIVCS-VKKRELEEMNFSGIGRKNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
            ++S   H LKAL+ S +S+V   + V TY F  +IGGQ+KV+++K +N KY + VEV  +
Sbjct: 60   QVSGRFHQLKALSASGISLVPTVKRVPTYLFRTDIGGQVKVYVEK-TNGKYKVLVEVLPV 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL ++ G+FRSD++CFMPLD  +  AD K STIETPF +   G + VELDF+ 
Sbjct: 119  ELSDAHAELVIVCGVFRSDASCFMPLDLNRCGADGKSSTIETPFVQGPSGKVTVELDFEA 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAP Y+S  +KSQL ++++ S IRSHR TNF+VPVGF SG PSPLG+SF  DG +NFA 
Sbjct: 179  SLAPCYISFYIKSQLVTDMESSEIRSHRNTNFVVPVGFSSGHPSPLGLSFLPDGSLNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FS + +SVVLCLY DT A+KP LEIDLDPYVNRSGDIWHA +DCSL F  YGYRC+  T 
Sbjct: 239  FSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVNRSGDIWHAALDCSLLFKTYGYRCKAATS 298

Query: 1813 NK-GHRVLLDPYARVIEDF-----WLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIY 1652
             K G  VLLDPYA+VI          +   K+LG+LC+EP +DWSGD RPSLPME+LIIY
Sbjct: 299  GKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGELCQEPGYDWSGDVRPSLPMEKLIIY 358

Query: 1651 RLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWH 1472
            RLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+LGVNA+LL+PIFPF+EQKGPY+P H
Sbjct: 359  RLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDLGVNAMLLQPIFPFEEQKGPYYPCH 418

Query: 1471 FFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYS 1292
            FFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV LEVVFTHT++ A L  +DN+SY  
Sbjct: 419  FFSPGNMYGRSGDALSVIKSVKDMVKKLHANGIEVFLEVVFTHTAQDAPLMHVDNTSY-C 477

Query: 1291 VNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRP 1112
            V GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF IDGF F+NASSL RG HGE LSRP
Sbjct: 478  VKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFRIDGFVFINASSLLRGFHGEILSRP 536

Query: 1111 PLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGP 932
            PL+EAIAFDP+LS VK+IAD+W+P + ++KE  FPHW+RWAE+N +FC D+R+FLRG+G 
Sbjct: 537  PLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPHWRRWAEINLRFCADIRDFLRGEGL 596

Query: 931  ISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGP 752
            +SNLATRLCGSGD+F  GRGPAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG 
Sbjct: 597  LSNLATRLCGSGDIFSGGRGPAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGA 656

Query: 751  TNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSG 572
            T  T+VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK  DWN LK+G
Sbjct: 657  TTNTIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTG 716

Query: 571  FGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKT 392
            FGIQ TQFI+FLS+L++RRSDLLQ  +FLKEENI W G +Q PPKWDD S KFL+MTLK 
Sbjct: 717  FGIQTTQFIAFLSNLRMRRSDLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKA 776

Query: 391  DAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDG 212
            DA+ SQ+    +  GGDLF+AFNS   SE V LPP   D  W  LVDTALPFPGFF   G
Sbjct: 777  DAEVSQTPVSDI--GGDLFVAFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKG 834

Query: 211  VPLEAGLATYEMKSHSCILFEARSL 137
             P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 835  TPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559779.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559780.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
          Length = 862

 Score = 1083 bits (2801), Expect = 0.0
 Identities = 543/865 (62%), Positives = 660/865 (76%), Gaps = 6/865 (0%)
 Frame = -1

Query: 2713 MAMLPVPYVMQSHCLSIEALESSKLFAASCGENGRRVTSDSNKLNLKQKTVHGQLMKPAR 2534
            MA  P+   + S  LS  + ES+KL  A  G  G+ V S   K  L++    G   K  +
Sbjct: 1    MATSPIQLAVHSRLLSYGSSESTKLVPAPPGNRGKIVCS-VKKRELEEMNFSGIGRKNDQ 59

Query: 2533 RISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVFIKKKSNNKYGLHVEVEFL 2354
            ++S   H LKAL+ S +S+V   + V TY F  +IGGQ+KV+++K +N KY + VEV  +
Sbjct: 60   QVSGRFHQLKALSASGISLVPTVKRVPTYLFRTDIGGQVKVYVEK-TNGKYKVLVEVLPV 118

Query: 2353 EHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIETPFTKNSVGTLAVELDFDE 2174
            E  +   EL ++ G+FRSD++CFMPLD  +  AD K STIETPF +   G + VELDF+ 
Sbjct: 119  ELSDAHAELVIVCGVFRSDASCFMPLDLNRCGADGKSSTIETPFVQGPSGKVTVELDFEA 178

Query: 2173 SLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGRPSPLGISFTDDGLINFAF 1994
            SLAP Y+S  +KSQL ++++ S IRSHR TNF+VPVGF SG PSPLG+SF  DG +NFA 
Sbjct: 179  SLAPCYISFYIKSQLVTDMESSEIRSHRNTNFVVPVGFSSGHPSPLGLSFLPDGSLNFAL 238

Query: 1993 FSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALVDCSLPFVGYGYRCRNGTE 1814
            FS + +SVVLCLY DT A+KP LEIDLDPYVNRSGDIWHA +DCSL F  YGYRC+  T 
Sbjct: 239  FSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVNRSGDIWHAALDCSLLFKTYGYRCKAATS 298

Query: 1813 NK-GHRVLLDPYARVIEDF-----WLDLPRKFLGKLCEEPAFDWSGDFRPSLPMERLIIY 1652
             K G  VLLDPYA+VI          +   K+LG+LC+EP +DWSGD RPSLPME+LIIY
Sbjct: 299  GKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGELCQEPGYDWSGDVRPSLPMEKLIIY 358

Query: 1651 RLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFPFDEQKGPYFPWH 1472
            RLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+LGVNA+LL+PIFPF+EQKGPY+P H
Sbjct: 359  RLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDLGVNAMLLQPIFPFEEQKGPYYPCH 418

Query: 1471 FFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYS 1292
            FFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV LEVVFTHT++ A L  +DN+SY  
Sbjct: 419  FFSPGNMYGRSGDALSVIKSIKDMVKKLHANGIEVFLEVVFTHTAQDAPLMHVDNTSY-C 477

Query: 1291 VNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFLSRP 1112
            V GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF IDGF F+NASSL RG HGE LSRP
Sbjct: 478  VKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFRIDGFVFINASSLLRGFHGEILSRP 536

Query: 1111 PLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFCVDVRNFLRGQGP 932
            PL+EAIAFDP+LS VK+IAD+W+P + ++KE  FPHW+RWAE+N +FC D+R+FLRG+G 
Sbjct: 537  PLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPHWRRWAEINLRFCADIRDFLRGEGL 596

Query: 931  ISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGP 752
            +SNLATRLCGSGD+F  GRGPAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG 
Sbjct: 597  LSNLATRLCGSGDIFSGGRGPAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGA 656

Query: 751  TNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTDRKPLDWNGLKSG 572
            T  T+VLE RLKQ+RNFLF+LFISLGVPVLNMGDECGQS+GGSP Y  RK  DWN LK+G
Sbjct: 657  TTNTIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTG 716

Query: 571  FGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDDESCKFLSMTLKT 392
            FGIQ TQFI+FLS+L++RRSDLLQ  +FLKEENI W G +Q PPKWDD S KFL+MTLK 
Sbjct: 717  FGIQTTQFIAFLSNLRMRRSDLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKA 776

Query: 391  DAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDTALPFPGFFTVDG 212
            DA+ SQ+    +  GGDLF+AFNS   SE V LPP   D  W  LVDTALPFPGFF   G
Sbjct: 777  DAEVSQTPVSDI--GGDLFVAFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKG 834

Query: 211  VPLEAGLATYEMKSHSCILFEARSL 137
             P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 835  TPVEDELVAYEMKSHSCLLFEAQRL 859