BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia30_contig00005932
         (3494 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum] >gi|11...  1427   0.0  
gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]      1376   0.0  
ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythra...  1209   0.0  
gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythra...  1209   0.0  
gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]   1194   0.0  
emb|CDP13767.1| unnamed protein product [Coffea canephora]           1162   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1141   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1129   0.0  
ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea...  1127   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1122   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1119   0.0  
dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]            1115   0.0  
dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. g...  1112   0.0  
dbj|BAG31374.1| topoisomerase I [Ophiorrhiza liukiuensis]            1096   0.0  
ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [N...  1064   0.0  
ref|XP_023553297.1| DNA topoisomerase 1 beta-like [Cucurbita pep...  1060   0.0  
ref|XP_023007686.1| DNA topoisomerase 1 beta-like [Cucurbita max...  1054   0.0  
ref|XP_022932254.1| DNA topoisomerase 1 beta-like [Cucurbita mos...  1051   0.0  
ref|XP_023748033.1| DNA topoisomerase 1 alpha-like [Lactuca sati...  1036   0.0  
gb|PON81509.1| DNA topoisomerase I [Trema orientalis]                1029   0.0  

>ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum]
 ref|XP_020553411.1| DNA topoisomerase 1 [Sesamum indicum]
          Length = 893

 Score = 1427 bits (3695), Expect = 0.0
 Identities = 724/893 (81%), Positives = 759/893 (84%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MA+E+C+ QKL        EPLVFKR           SE K+ LSQKHDR L RP+PDVR
Sbjct: 1    MAIESCNDQKLMDDMDEDDEPLVFKRSSSSAKQSQGNSETKRPLSQKHDRPLNRPSPDVR 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
             QNGQST+++KSKTVP S+TPP++ PL SPKSSN+S KALA++  EVD +PSTSVTD+SN
Sbjct: 61   PQNGQSTMIQKSKTVPSSRTPPLRSPLASPKSSNSSAKALAVRPREVDPRPSTSVTDESN 120

Query: 2801 SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 2622
            SVKHQIKDEK ST+ATG  NEDS+DSEDDKPLSARLPAGLSKVNSNHA K PN  T  QK
Sbjct: 121  SVKHQIKDEKPSTKATGGLNEDSEDSEDDKPLSARLPAGLSKVNSNHANKGPNALTSVQK 180

Query: 2621 RRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTERKPSTN 2442
             +IP PEDSDDEIPLSSKFQVKSNAGES NR S SGERKPLL+KYDQNGSAS ERKPST 
Sbjct: 181  TKIPKPEDSDDEIPLSSKFQVKSNAGESTNRPSISGERKPLLAKYDQNGSASAERKPSTI 240

Query: 2441 LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMXXXXX 2262
            LKKRP DGVK+  ESYLKKPKLS+A+TP+K KE SIK EM++EDDEDHVPIS R+     
Sbjct: 241  LKKRPADGVKSAGESYLKKPKLSDASTPNKTKETSIKDEMKDEDDEDHVPISQRIKQQTS 300

Query: 2261 XXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVI 2082
                    KN+TKPI                             SGEGQKWTTLVHNGVI
Sbjct: 301  SDKKSSSAKNMTKPISASAKKTSKKPKKVMKNSKYSKSSKVPPSSGEGQKWTTLVHNGVI 360

Query: 2081 FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKILGK 1902
            FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVML+TDYMNKPKFKENFMADWRKILGK
Sbjct: 361  FPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLDTDYMNKPKFKENFMADWRKILGK 420

Query: 1901 NHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKV 1722
            NH IQSLEHCDFTPIYEWH                           KYMWAIVNGVKEKV
Sbjct: 421  NHVIQSLEHCDFTPIYEWHQKEKEKKKQMTAEEKKALKEEKLKQEEKYMWAIVNGVKEKV 480

Query: 1721 GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT 1542
            GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT
Sbjct: 481  GNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNT 540

Query: 1541 VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSK 1362
            VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYI GIRAAYTKDFT+K
Sbjct: 541  VTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNK 600

Query: 1361 DPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDS 1182
            DP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDFLGKDS
Sbjct: 601  DPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDS 660

Query: 1181 IRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNA 1002
            IRYQNEVEVET VFKAIQQFRSGKKG DD+FDRLDTSKLNAHLKELMPGLTAKVFRTYNA
Sbjct: 661  IRYQNEVEVETPVFKAIQQFRSGKKGSDDMFDRLDTSKLNAHLKELMPGLTAKVFRTYNA 720

Query: 1001 SITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELK 822
            SITLDD LSRET+GGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQM RLNEKIEELK
Sbjct: 721  SITLDDKLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMTRLNEKIEELK 780

Query: 821  GVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKEDL 642
            GV+EELQTDLARAKKGKPPLKSADGKPKRNLNPDALE+KIAQT AKIEKMERDKETKEDL
Sbjct: 781  GVLEELQTDLARAKKGKPPLKSADGKPKRNLNPDALEKKIAQTNAKIEKMERDKETKEDL 840

Query: 641  KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 841  KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPSFRF 893


>gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]
          Length = 895

 Score = 1376 bits (3562), Expect = 0.0
 Identities = 701/895 (78%), Positives = 747/895 (83%), Gaps = 2/895 (0%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEACDKQKL        EPLVFKR           SE KKSLSQKHDRQLG+PAPD+R
Sbjct: 1    MAVEACDKQKLMDDMDDDDEPLVFKRSSSAAKQNQSVSETKKSLSQKHDRQLGKPAPDIR 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
            SQNGQST+V+KSK    SKTPPV+ PL SPKSSN+S K+ A+KS  V+SKPSTSV D+SN
Sbjct: 61   SQNGQSTMVEKSKMASSSKTPPVRSPLASPKSSNSSAKSFAVKSSGVNSKPSTSVADESN 120

Query: 2801 SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 2622
            SVK Q +D++  TEAT E NE+S+DS+DDKPL+ARL AG SK+NSNHA K PN S L QK
Sbjct: 121  SVKQQSRDKRPLTEATAELNEESEDSDDDKPLTARLSAGFSKINSNHANKGPNASILGQK 180

Query: 2621 RRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTERKPSTN 2442
             +IP  EDSDDEIPLSSKFQVKSNAG+S  +S  SG RKPL +K D NG  S ERKPS++
Sbjct: 181  PKIPKSEDSDDEIPLSSKFQVKSNAGDSSKKSPTSGGRKPLSAKCDHNGLGSPERKPSSS 240

Query: 2441 --LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMXXX 2268
              LKKRP DGVK+  ES+LK+PKLS+A+  +KNKE SIKAE++EEDDEDHVPIS RM   
Sbjct: 241  TILKKRPADGVKSAGESHLKRPKLSDASASNKNKETSIKAELKEEDDEDHVPISARMKKS 300

Query: 2267 XXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQKWTTLVHNG 2088
                      KNITKP+                             SGEGQKWTTLVHNG
Sbjct: 301  TSSDKKPSSSKNITKPVSSLSKKINKKSKKVTKKSEYSKSSKVPPGSGEGQKWTTLVHNG 360

Query: 2087 VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKIL 1908
            VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATM+AVML+TDYMNKPKFKENFM DWRKIL
Sbjct: 361  VIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMNKPKFKENFMDDWRKIL 420

Query: 1907 GKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVNGVKE 1728
            GKNH IQSLEHC+FTPIYEWH                           KYMWAIV+GVKE
Sbjct: 421  GKNHVIQSLEHCNFTPIYEWHQKEKEKKKQMSADEKKALKEEKLKQEDKYMWAIVDGVKE 480

Query: 1727 KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHD 1548
            KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDI INIGKDAPIPECPIPGESWKE+RHD
Sbjct: 481  KVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDIVINIGKDAPIPECPIPGESWKEVRHD 540

Query: 1547 NTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFT 1368
            NTVTWLAYWNDPINPKEFKYVFLAASS LKGQSDKEKYEKARLLKDYI  IRA YTK+FT
Sbjct: 541  NTVTWLAYWNDPINPKEFKYVFLAASSALKGQSDKEKYEKARLLKDYIQDIRATYTKNFT 600

Query: 1367 SKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGK 1188
            SKDP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDFLGK
Sbjct: 601  SKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGK 660

Query: 1187 DSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTY 1008
            DSIRYQNEVEVETAVF+AIQ+FR GKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVFRTY
Sbjct: 661  DSIRYQNEVEVETAVFRAIQEFRRGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTY 720

Query: 1007 NASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE 828
            NASITLDDMLSRET+GGDVAEKVVVYQ ANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE
Sbjct: 721  NASITLDDMLSRETKGGDVAEKVVVYQLANKEVAIICNHQRTVSKSHSAQMLRLNEKIEE 780

Query: 827  LKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE 648
            LKGV+EELQTDLARAKKGKPPLK+ADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE
Sbjct: 781  LKGVLEELQTDLARAKKGKPPLKNADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKE 840

Query: 647  DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 841  DLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPSFRF 895


>ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythranthe guttata]
          Length = 894

 Score = 1209 bits (3127), Expect = 0.0
 Identities = 635/909 (69%), Positives = 701/909 (77%), Gaps = 16/909 (1%)
 Frame = -1

Query: 3161 MAVEAC-DKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDV 2985
            M VEA  DK  +         PLVFKR            E K S SQKH+ Q+GR + DV
Sbjct: 1    MTVEAREDKNMMEDMDDDDDAPLVFKRSGSSSKPKQSEPENKLS-SQKHNAQVGRQSSDV 59

Query: 2984 RSQNGQSTIVKKSKTVPLSKT--PPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTD 2811
            R QNG +   +K K+V  S +  PPV+  L SPKSSN+S KA  +KSP+V+SKPSTSV  
Sbjct: 60   RPQNGTNANAQKGKSVTSSSSMKPPVRPSLVSPKSSNSSVKASPIKSPDVNSKPSTSVVG 119

Query: 2810 KSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTL 2631
            +S SVK  IK+E            +S+DSEDDKPLSAR  AG SK N N   K PN   L
Sbjct: 120  ESKSVKSTIKEE------------NSEDSEDDKPLSARQVAGSSKNNPNQTNK-PNAPNL 166

Query: 2630 SQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLS-KYDQNGSASTERK 2454
             +K  +P PEDSDDEIPLS+KFQ KSNAG S +RSS+S E+KPL++ K D+NGSASTERK
Sbjct: 167  GKKSNVPKPEDSDDEIPLSAKFQGKSNAGASTSRSSHSEEKKPLVARKPDKNGSASTERK 226

Query: 2453 PSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMX 2274
            PST L+KRP DGV +  +S LKKPKLS++    K+K+AS+K E++ EDDED VP+S R  
Sbjct: 227  PSTVLQKRPADGVASAGQSNLKKPKLSDSLAS-KDKKASVKPEVKAEDDEDLVPLSQRTK 285

Query: 2273 XXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXS----------- 2127
                          +TK                               +           
Sbjct: 286  KSTTPEKKPSPALKVTKSTASISKKTNNKTNSKTNSKTNKKSKQVSKDNNYSKSSKLPPS 345

Query: 2126 -GEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKP 1950
             G+GQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPV+LTPEQEEVATMYAVMLETDY+ KP
Sbjct: 346  SGDGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVNLTPEQEEVATMYAVMLETDYLKKP 405

Query: 1949 KFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXX 1770
            +FKENFM DW+KILGKNH IQSLEHCDFTPIYEWH                         
Sbjct: 406  QFKENFMTDWKKILGKNHVIQSLEHCDFTPIYEWHQREKEKKKQMSTDEKKALKEEKLKL 465

Query: 1769 XXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPE 1590
              KYMWA+++GV+EKVGNFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIG+DAPIPE
Sbjct: 466  EEKYMWAVLDGVREKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGRDAPIPE 525

Query: 1589 CPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKD 1410
            CPI GE WKEIRHDNTVTWLAYW+DPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLKD
Sbjct: 526  CPIEGERWKEIRHDNTVTWLAYWSDPINGKEFKYVFLAASSTLKGQSDKEKYEKARLLKD 585

Query: 1409 YILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 1230
            YI GIR AYT+DF SKDPAK+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE
Sbjct: 586  YIQGIRTAYTRDFVSKDPAKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 645

Query: 1229 PVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLK 1050
            PVPPN LKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFD+LDTSKLNAHLK
Sbjct: 646  PVPPNILKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDKLDTSKLNAHLK 705

Query: 1049 ELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKS 870
            ELMPGLTAKVFRTYNASITLDD LSRET+GGD+AEKVV Y HANKEVAIICNHQRTVSK+
Sbjct: 706  ELMPGLTAKVFRTYNASITLDDKLSRETKGGDLAEKVVTYNHANKEVAIICNHQRTVSKT 765

Query: 869  HSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTT 690
            HSAQMLRL+EKI+ELKGV+EEL+TDL RA+KGKP L  ADGK KRN+NP+ALERKIAQTT
Sbjct: 766  HSAQMLRLDEKIDELKGVLEELKTDLGRARKGKPLLAGADGKAKRNINPEALERKIAQTT 825

Query: 689  AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWA 510
            AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSLL KF+WA
Sbjct: 826  AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKMFNKSLLEKFSWA 885

Query: 509  MDVDPGFRF 483
            MDVDP FRF
Sbjct: 886  MDVDPSFRF 894


>gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythranthe guttata]
          Length = 883

 Score = 1209 bits (3127), Expect = 0.0
 Identities = 635/898 (70%), Positives = 699/898 (77%), Gaps = 5/898 (0%)
 Frame = -1

Query: 3161 MAVEAC-DKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDV 2985
            M VEA  DK  +         PLVFKR            E K S SQKH+ Q+GR + DV
Sbjct: 1    MTVEAREDKNMMEDMDDDDDAPLVFKRSGSSSKPKQSEPENKLS-SQKHNAQVGRQSSDV 59

Query: 2984 RSQNGQSTIVKKSKTVPLSKT--PPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTD 2811
            R QNG +   +K K+V  S +  PPV+  L SPKSSN+S KA  +KSP+V+SKPSTSV  
Sbjct: 60   RPQNGTNANAQKGKSVTSSSSMKPPVRPSLVSPKSSNSSVKASPIKSPDVNSKPSTSVVG 119

Query: 2810 KSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTL 2631
            +S SVK  IK+E            +S+DSEDDKPLSAR  AG SK N N   K PN   L
Sbjct: 120  ESKSVKSTIKEE------------NSEDSEDDKPLSARQVAGSSKNNPNQTNK-PNAPNL 166

Query: 2630 SQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLS-KYDQNGSASTERK 2454
             +K  +P PEDSDDEIPLS+KFQ KSNAG S +RSS+S E+KPL++ K D+NGSASTERK
Sbjct: 167  GKKSNVPKPEDSDDEIPLSAKFQGKSNAGASTSRSSHSEEKKPLVARKPDKNGSASTERK 226

Query: 2453 PSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRMX 2274
            PST L+KRP DGV +  +S LKKPKLS++    K+K+AS+K E++ EDDED VP+S R  
Sbjct: 227  PSTVLQKRPADGVASAGQSNLKKPKLSDSLAS-KDKKASVKPEVKAEDDEDLVPLSQRTK 285

Query: 2273 XXXXXXXXXXXXKNIT-KPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQKWTTLV 2097
                             K                               SG+GQKWTTLV
Sbjct: 286  KSTTPEKKPSPALKTNNKTNSKTNSKTNKKSKQVSKDNNYSKSSKLPPSSGDGQKWTTLV 345

Query: 2096 HNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWR 1917
            HNGVIFPPPYKPHGVKMLYKGKPV+LTPEQEEVATMYAVMLETDY+ KP+FKENFM DW+
Sbjct: 346  HNGVIFPPPYKPHGVKMLYKGKPVNLTPEQEEVATMYAVMLETDYLKKPQFKENFMTDWK 405

Query: 1916 KILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVNG 1737
            KILGKNH IQSLEHCDFTPIYEWH                           KYMWA+++G
Sbjct: 406  KILGKNHVIQSLEHCDFTPIYEWHQREKEKKKQMSTDEKKALKEEKLKLEEKYMWAVLDG 465

Query: 1736 VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEI 1557
            V+EKVGNFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIG+DAPIPECPI GE WKEI
Sbjct: 466  VREKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGRDAPIPECPIEGERWKEI 525

Query: 1556 RHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTK 1377
            RHDNTVTWLAYW+DPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLKDYI GIR AYT+
Sbjct: 526  RHDNTVTWLAYWSDPINGKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIQGIRTAYTR 585

Query: 1376 DFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDF 1197
            DF SKDPAK+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDF
Sbjct: 586  DFVSKDPAKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDF 645

Query: 1196 LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVF 1017
            LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVF
Sbjct: 646  LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVF 705

Query: 1016 RTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEK 837
            RTYNASITLDD LSRET+GGD+AEKVV Y HANKEVAIICNHQRTVSK+HSAQMLRL+EK
Sbjct: 706  RTYNASITLDDKLSRETKGGDLAEKVVTYNHANKEVAIICNHQRTVSKTHSAQMLRLDEK 765

Query: 836  IEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKE 657
            I+ELKGV+EEL+TDL RA+KGKP L  ADGK KRN+NP+ALERKIAQTTAKIEKMERDKE
Sbjct: 766  IDELKGVLEELKTDLGRARKGKPLLAGADGKAKRNINPEALERKIAQTTAKIEKMERDKE 825

Query: 656  TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSLL KF+WAMDVDP FRF
Sbjct: 826  TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKMFNKSLLEKFSWAMDVDPSFRF 883


>gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]
          Length = 870

 Score = 1194 bits (3089), Expect = 0.0
 Identities = 631/898 (70%), Positives = 700/898 (77%), Gaps = 5/898 (0%)
 Frame = -1

Query: 3161 MAVEACDKQK-LXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDV 2985
            MAV+A D++K L        +PLVFKR                S S KH+ + G+     
Sbjct: 1    MAVDAFDEKKSLDDVDDDDDQPLVFKR---------------SSSSSKHNLKNGQ----- 40

Query: 2984 RSQNGQSTIVKKSKTVP-LSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDK 2808
            +S+NG+S+ V+K   VP  SK PP+K PLTSPKSS+++ K   +KS   +SK   S TDK
Sbjct: 41   QSRNGESSSVQKGTLVPPSSKAPPIKSPLTSPKSSSSAAKESPVKSSAANSKRPMSETDK 100

Query: 2807 SNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 2628
             NSVK Q+K+E+   +A  E + D +DS DD PLSARL A  SK NSNHAK+ PN   L 
Sbjct: 101  PNSVKQQVKEEQPFAQAADETH-DLEDSGDDTPLSARLLASSSKGNSNHAKQRPNALALV 159

Query: 2627 QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE---R 2457
            QK  IP  EDSDDE+PLSSKFQVKS+   S +RS+NS ERKP+L+K DQNGS+ST+   +
Sbjct: 160  QKNSIPKKEDSDDEMPLSSKFQVKSDVRVSTSRSNNSDERKPILNKADQNGSSSTDHSQK 219

Query: 2456 KPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRM 2277
            KPS  +K RP + VK    S  KKPKLS A++      A  +A+ + EDD+DHVPIS RM
Sbjct: 220  KPSIAMK-RPPNPVKVECRSPEKKPKLSNASS------AIPRAKPKAEDDDDHVPISQRM 272

Query: 2276 XXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQKWTTLV 2097
                         K +TK +                             SGEGQKWTTLV
Sbjct: 273  KNQSSSEKKSLSVKKVTKSVSAVATKTNKISKKIVKNTKYSISSKVLPNSGEGQKWTTLV 332

Query: 2096 HNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWR 1917
            HNGVIFPPPYKPHGVKMLYKGKPV LTPEQEEVATM+AVML+TDYMNKPKFKENFM DWR
Sbjct: 333  HNGVIFPPPYKPHGVKMLYKGKPVVLTPEQEEVATMFAVMLDTDYMNKPKFKENFMTDWR 392

Query: 1916 KILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVNG 1737
            KILGKNHTIQ LE CDFTPIYEWH                           KYMWAI++G
Sbjct: 393  KILGKNHTIQKLEDCDFTPIYEWHQKEKEKKKQMSTEEKKALKEEKLKQEEKYMWAILDG 452

Query: 1736 VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEI 1557
            VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI+PSDI INIGKDA IPECPIPGESWKEI
Sbjct: 453  VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIQPSDIIINIGKDAAIPECPIPGESWKEI 512

Query: 1556 RHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTK 1377
            RHDNTVTWLAYWNDPIN KEFKYVFLAASSTLKGQSDKEKYEKARLLK+YI GIRAAYTK
Sbjct: 513  RHDNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKNYIQGIRAAYTK 572

Query: 1376 DFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDF 1197
            DF+SKDP ++QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPN LKFDF
Sbjct: 573  DFSSKDPTRKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDF 632

Query: 1196 LGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVF 1017
            LGKDSIRYQNEV VETAVFKAIQQFR+GKKGGDDLFD+LDTSKLNAHLKELMPGLTAKVF
Sbjct: 633  LGKDSIRYQNEVVVETAVFKAIQQFRNGKKGGDDLFDKLDTSKLNAHLKELMPGLTAKVF 692

Query: 1016 RTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEK 837
            RTYNASITLDDML+RET+GG+VAEK+VVYQHANKEVAIICNHQRTVSKSHSAQMLRL+EK
Sbjct: 693  RTYNASITLDDMLNRETKGGEVAEKIVVYQHANKEVAIICNHQRTVSKSHSAQMLRLDEK 752

Query: 836  IEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKE 657
            I+ELKG +EEL+ DL+RAKKGKPPLKSADGKPKRN+NP+ALE+KIAQT AKIEKMERDKE
Sbjct: 753  IDELKGAMEELREDLSRAKKGKPPLKSADGKPKRNMNPEALEKKIAQTAAKIEKMERDKE 812

Query: 656  TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            TKEDLKT+ALGTS+INYLDPRITVAWCKR EVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 813  TKEDLKTIALGTSRINYLDPRITVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPEFRF 870


>emb|CDP13767.1| unnamed protein product [Coffea canephora]
          Length = 926

 Score = 1162 bits (3006), Expect = 0.0
 Identities = 613/927 (66%), Positives = 682/927 (73%), Gaps = 34/927 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         PLVFKR           SE KK  SQK D Q  R + DV 
Sbjct: 1    MAVEACSTPSLLEDSDEENAPLVFKRTSSSSKQNLSNSETKKLSSQKRDGQSSRNSSDVC 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NGQS+  +  + V  +KTPP K PL+SPK++N S K   + SP  +SKPS+S +    
Sbjct: 61   PVNGQSSSAQTGRVVSSAKTPPAKSPLSSPKAANISAKTSLITSPGANSKPSSSTSATVK 120

Query: 2801 SVKHQ-----IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTS 2637
             +++      +K  K S  A  + +   +DS+DDKPL A+LP+G S+   NH  K  + S
Sbjct: 121  QLENHKNSPVVKLPKRSVSAA-KLSSTPEDSDDDKPLMAKLPSGSSRAIVNHGNKDGDGS 179

Query: 2636 TLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER 2457
            +L QK  +   EDSDDE PLSS++  KSN  ESI++S N GE+K L +K  QNGSA  + 
Sbjct: 180  SLVQKPGVEEDEDSDDEKPLSSRYLSKSNLVESISKSRNPGEKKSLPAKVQQNGSALRDG 239

Query: 2456 ---KPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDED----- 2301
               K S+   KRP   VK++DES +KKPKLS        K+A++KAE + EDD+D     
Sbjct: 240  PVGKSSSLSNKRPPGEVKSSDESAVKKPKLSGPTAAINKKQATLKAEPKVEDDDDDILIS 299

Query: 2300 ---------------------HVPISHRMXXXXXXXXXXXXXKNITKPIXXXXXXXXXXX 2184
                                 HVPI+ RM             K + K +           
Sbjct: 300  QTAKKMFASASKPKSNDDDDDHVPITQRMKKSTTSVSQSSSVKKVAKVVSSSLKKTNKKS 359

Query: 2183 XXXXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQE 2004
                              SGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKG+PV LTPEQE
Sbjct: 360  KKIIKKSKYSKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGQPVTLTPEQE 419

Query: 2003 EVATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXX 1824
            EVATM+AVML+TDYM+KP+FKENF +DW++ILGKNHTIQ+LE CDF PIYEWH       
Sbjct: 420  EVATMFAVMLDTDYMSKPQFKENFFSDWKRILGKNHTIQNLEDCDFRPIYEWHQKEKEKK 479

Query: 1823 XXXXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 1644
                                KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR
Sbjct: 480  KQMTTEEKKSLKEEKLKQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 539

Query: 1643 IRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASST 1464
            IRP DITINIGKDAPIPECPIPGE WKEIRHDNTVTWLA+WNDPINPKEFKYVFLAASST
Sbjct: 540  IRPRDITINIGKDAPIPECPIPGERWKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASST 599

Query: 1463 LKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKD 1284
            LKGQSDKEKYEKARLLKDYI GIR AYTKDF SKDP K+QIAVATYLIDKLALRAGNEKD
Sbjct: 600  LKGQSDKEKYEKARLLKDYIHGIRTAYTKDFASKDPMKKQIAVATYLIDKLALRAGNEKD 659

Query: 1283 DDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKG 1104
            DDEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEVEVE+ VFKAIQQFRSGK+G
Sbjct: 660  DDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVESGVFKAIQQFRSGKEG 719

Query: 1103 GDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQH 924
             +DLFD+LDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSR+T GGDV EKVVVYQ 
Sbjct: 720  SEDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRKTNGGDVLEKVVVYQQ 779

Query: 923  ANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGK 744
            ANKEVAIICNHQRTVSK+HSAQM RLNEKIEELKGV+EEL+TDL+RAKKGKPPLK+ADGK
Sbjct: 780  ANKEVAIICNHQRTVSKTHSAQMSRLNEKIEELKGVLEELKTDLSRAKKGKPPLKNADGK 839

Query: 743  PKRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE 564
            PKRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE
Sbjct: 840  PKRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHE 899

Query: 563  VPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            VPIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  VPIEKIFNKSLLAKFAWSMDVDPSFRF 926


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1141 bits (2952), Expect = 0.0
 Identities = 605/903 (66%), Positives = 680/903 (75%), Gaps = 10/903 (1%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAV+AC K+K+        EPLVFKR           S+ KKS SQ++D Q GR   DV 
Sbjct: 1    MAVQACVKEKMMDEIDEDDEPLVFKRSNATSKHNQLNSDTKKS-SQRYDGQSGRQVSDVP 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
            S NGQS+   K KT  LS    +K P+ SPK+S +  KA  + +P+V S  S+    K +
Sbjct: 60   STNGQSSSASKGKTTKLSS---MKSPIASPKASTSFAKASPVANPKVSS--SSDDRSKHS 114

Query: 2801 SVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQK 2622
            S ++ I   K   E      E   DSED+KPLSARL  GL+K +SN+A K    S+ +  
Sbjct: 115  SKQNTINVVKEEKELVNPAAEPYGDSEDEKPLSARLFTGLTKGSSNNANKGLINSSPAS- 173

Query: 2621 RRIPIP--------EDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSAS 2466
              +P+P        +DSDDEIPLSSKF++K+NAG S  +S  S + K L+S + QNGS +
Sbjct: 174  --LPVPKPEINRYSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSIN 231

Query: 2465 TERKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREED-DEDHVPI 2289
               K S  + KRP   VK++ +S +KKPKLS+A+TP  NK+AS KAE + +D D+DHVPI
Sbjct: 232  RGSKSSIKVNKRPLGEVKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDDHVPI 291

Query: 2288 SHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQK 2112
            S RM             K  +TK +                             SGEG+K
Sbjct: 292  SQRMKKSATSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSGEGKK 351

Query: 2111 WTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENF 1932
            W TLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATM+AVML+TDYM K KFKENF
Sbjct: 352  WNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKFKENF 411

Query: 1931 MADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMW 1752
            M DWRKILGKNH IQ+LE CDFTPIYEWH                           KYMW
Sbjct: 412  MDDWRKILGKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEEKYMW 471

Query: 1751 AIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGE 1572
            AIVNGVKEKVGNFRVEPP LFRGRGEHPKMGKLKK IRPSDITINIGKDAPIPECPIPGE
Sbjct: 472  AIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECPIPGE 531

Query: 1571 SWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIR 1392
            SWKEIRHDNTVTWLA+WNDPI P+EFKYVFLAASS+LKGQSDKEKYEKARLLKD+I GIR
Sbjct: 532  SWKEIRHDNTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFIQGIR 591

Query: 1391 AAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNT 1212
            AAYTKDF SKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PP+ 
Sbjct: 592  AAYTKDFASKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPSI 651

Query: 1211 LKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGL 1032
            LKFDFLGKDSIRYQNEVEVE  V+KAIQQFR+GK+GGDDLFD+LDTSKLNAHLK LMPGL
Sbjct: 652  LKFDFLGKDSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGLMPGL 711

Query: 1031 TAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQML 852
            TAKVFRTYNASITLD+MLSRET+GG+VAEK+VVYQHANKEVAIICNHQRTVSKSH AQM 
Sbjct: 712  TAKVFRTYNASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHPAQMT 771

Query: 851  RLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKM 672
            RLN KI+ELKG+++ LQTDLARAKKGKPPL+ ADGKPKRNL P+ALERKI QT AKIEKM
Sbjct: 772  RLNGKIDELKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAKIEKM 831

Query: 671  ERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPG 492
            ERDKETKE LKT+ALGTSKI+YLDPRITVAWCKRHEVPIEK+FNKSLLAKFAW+MDVDP 
Sbjct: 832  ERDKETKEGLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMDVDPS 891

Query: 491  FRF 483
            FRF
Sbjct: 892  FRF 894


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1129 bits (2921), Expect = 0.0
 Identities = 605/919 (65%), Positives = 667/919 (72%), Gaps = 26/919 (2%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L        EP+VFKR           SE +K  SQK D Q  R   D R
Sbjct: 1    MAVEACPTPNLRDDMDEDDEPIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSR 60

Query: 2981 SQNGQSTIVKKSKTVPLSK---------TPP-----VKYPLTSPKSSNTSGKALALKSPE 2844
              NG+S+  +K + VP SK         +PP     VK PL+SPK S +S K   + S  
Sbjct: 61   PANGESSSGQKGRMVPPSKPSQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLP 120

Query: 2843 VDSKPSTSVTDKSNSVKHQ-----IKDEKLST-EATGEPNEDSQDSEDDKPLSARLPAGL 2682
             +SKPSTS + K            IK+EK S          DS+DS+D KPLS R  A  
Sbjct: 121  ANSKPSTSGSFKVKPFNQHKSATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLRHSASS 180

Query: 2681 SKVNSNHAKKSPNTSTLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKP 2502
            SK N+N   K  +          P  EDSDDE PLSS+F +KS  GES +++ +S + KP
Sbjct: 181  SKGNTNQVSKEGSK---------PENEDSDDEKPLSSRFPLKSGVGESTSKAYDSDDDKP 231

Query: 2501 LLSKYDQNGSASTERKP---STNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIK 2331
            L  K   NGSA  + +P   S    KRP   +K++++  +KKPKLS    P   K+AS+K
Sbjct: 232  LAKKLQHNGSAMRDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPTNKKQASVK 291

Query: 2330 AEMR-EEDDEDHVPISHRMXXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXX 2154
             E + E+DD+DH+PIS R+             K  TK +                     
Sbjct: 292  PEPKAEDDDDDHLPISQRIKKSPTSESKSSSIKKTTKVVSSSVKKVNKKSKKVMKNSKYS 351

Query: 2153 XXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVML 1974
                    SGEG+KW  L HNGVIFPPPYKPHG+KMLYKGKPVDLTPEQEEVATM+AVML
Sbjct: 352  KSSKVPPSSGEGKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEEVATMFAVML 411

Query: 1973 ETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXX 1794
            +TDYMNKP+FKENF  DW+KILGKNH IQ+LE+CDF+PIYEWH                 
Sbjct: 412  DTDYMNKPRFKENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKKQMTTEEKKA 471

Query: 1793 XXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINI 1614
                      KYMWA V+G KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRP DITINI
Sbjct: 472  LKEEKLKQEEKYMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINI 531

Query: 1613 GKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKY 1434
            GKDAPIPECP+PGE WKE+RHDNTVTWLA+WNDPINPKEFKYVFLAASSTLKG SDKEKY
Sbjct: 532  GKDAPIPECPVPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTLKGLSDKEKY 591

Query: 1433 EKARLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCC 1254
            EKARLLKDYI GIRAAYTKDF SKDP K+QIAVATYLIDKLALRAGNEKDDDEADTVGCC
Sbjct: 592  EKARLLKDYIQGIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCC 651

Query: 1253 TLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDT 1074
            TLKVENVEPVPPN LKFDFLGKDSIRYQNEVEVE AVFKAIQQFRSGK+G  DLFD LDT
Sbjct: 652  TLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGSSDLFDLLDT 711

Query: 1073 SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICN 894
            SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRET+GGDVAEKVVVYQHANKEVAIICN
Sbjct: 712  SKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHANKEVAIICN 771

Query: 893  HQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKS--ADGKPKRNLNPD 720
            HQRTVSKSHSAQMLRLNEKIEELK VVEEL++DL+R KKGKPPLKS  ADGKPKRNLNP+
Sbjct: 772  HQRTVSKSHSAQMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADGKPKRNLNPE 831

Query: 719  ALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN 540
            +LE+KIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN
Sbjct: 832  SLEKKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFN 891

Query: 539  KSLLAKFAWAMDVDPGFRF 483
            KSLLAKF WAMDVDP FRF
Sbjct: 892  KSLLAKFTWAMDVDPSFRF 910


>ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea var. sylvestris]
          Length = 771

 Score = 1127 bits (2915), Expect = 0.0
 Identities = 576/772 (74%), Positives = 624/772 (80%), Gaps = 4/772 (0%)
 Frame = -1

Query: 2786 IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQKRRIPI 2607
            +K EK ST    EPN DS+DSEDDKPLSARL   LSK NSN+A K  N S L QK  IP 
Sbjct: 1    MKVEKPSTGPAAEPN-DSEDSEDDKPLSARLSTSLSKNNSNNASKGSNASRLGQKPSIPK 59

Query: 2606 PEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSA---STERKPSTNLK 2436
             EDSDDE PLS K Q+KSN G S  +S +S E+  L +K +QNGS    S  +KPST L 
Sbjct: 60   QEDSDDEKPLSHKVQLKSNVGSSTRKSYDSDEKMHLAAKNNQNGSPLQDSGSKKPSTILS 119

Query: 2435 KRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMR-EEDDEDHVPISHRMXXXXXX 2259
            KRP   V +  +S +KK K+S A+    NK+AS+KAE + E+DD+DH+PIS RM      
Sbjct: 120  KRPASEVNSAGQSSIKKHKISNASVLINNKQASVKAEPKVEDDDDDHIPISQRMKKPAAS 179

Query: 2258 XXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIF 2079
                   K +TK +                             SGEGQKWTTL+HNGVIF
Sbjct: 180  DKKISAIKKVTKVVSSSLNKTNKNSKKVVKKSQYSKSSKVPPGSGEGQKWTTLIHNGVIF 239

Query: 2078 PPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADWRKILGKN 1899
            PPPY+ HGVKMLYKGKPV+LTPEQEEVATM+AVML+T+YMNKPKFKENFM+DWRKILGKN
Sbjct: 240  PPPYQSHGVKMLYKGKPVNLTPEQEEVATMFAVMLDTEYMNKPKFKENFMSDWRKILGKN 299

Query: 1898 HTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVG 1719
            H IQ+LE CDFTPIYEWH                           KYMWAIV+GVKEKVG
Sbjct: 300  HIIQNLEDCDFTPIYEWHQKEKEKKKQMTTEEKKVIKEEKMKQEEKYMWAIVDGVKEKVG 359

Query: 1718 NFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV 1539
            NFRVEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV
Sbjct: 360  NFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKDAPIPECPIPGESWKEIRHDNTV 419

Query: 1538 TWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYTKDFTSKD 1359
            TWLA+WNDPINPKE KYVFLAASSTLKGQSDKEKYEK+RLLKDYI GIRA+YTKDF SKD
Sbjct: 420  TWLAFWNDPINPKEIKYVFLAASSTLKGQSDKEKYEKSRLLKDYIQGIRASYTKDFASKD 479

Query: 1358 PAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSI 1179
            P K+QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP+PPN LKFDFLGKDSI
Sbjct: 480  PTKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPMPPNILKFDFLGKDSI 539

Query: 1178 RYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNAS 999
            RYQNEVEVE+AVFKAIQQFRSGKK  DDLFD+LDTSKLNAHLKELMPGLTAKVFRTYNAS
Sbjct: 540  RYQNEVEVESAVFKAIQQFRSGKKAADDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNAS 599

Query: 998  ITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKG 819
            ITLD+MLS++T+ GDVAE +VVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELK 
Sbjct: 600  ITLDEMLSKDTKAGDVAENIVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKV 659

Query: 818  VVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDKETKEDLK 639
            V+EEL TDL RAKKGKPPLKSADGK KRNLNP+ALE+KIAQT AKIEKMERDKETKEDLK
Sbjct: 660  VLEELTTDLVRAKKGKPPLKSADGKTKRNLNPEALEKKIAQTNAKIEKMERDKETKEDLK 719

Query: 638  TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKF WAMDVDP FRF
Sbjct: 720  TVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFTWAMDVDPSFRF 771


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1122 bits (2901), Expect = 0.0
 Identities = 598/926 (64%), Positives = 667/926 (72%), Gaps = 33/926 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         P++FKR           SE KK   QK D Q      DVR
Sbjct: 1    MAVEACTTN-LMEDMEDDGGPVIFKRSKPAPKQNQANSEKKKLSLQKQDGQSAVHTSDVR 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NG+S+  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2801 SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 2634
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 179

Query: 2633 LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 2460
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGSAS +  
Sbjct: 180  SIRRPVIEESEDSEDEKPLSCRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSASRDGP 239

Query: 2459 -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 2304
              K ++   KRP    K+ ++S +KKPKLS   T   NK+A++K E + EDD+       
Sbjct: 240  MNKSASLSNKRPPGEAKSLNQSSVKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 299

Query: 2303 -----------------DHVPISHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXX 2178
                             D+VPIS R+                 +  +             
Sbjct: 300  RGKKSQSATSKPKDDDDDNVPISQRIKKSPASVSKSSVPVKKASTVVSSSLKKVNKNSKK 359

Query: 2177 XXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1998
                            SGEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 360  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 419

Query: 1997 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1818
            ATM+AVML+TDYMNKP FKENF +DW+KILGKNH IQ+LE CDF PIYEWH         
Sbjct: 420  ATMFAVMLDTDYMNKPCFKENFFSDWKKILGKNHMIQNLEDCDFGPIYEWHQQEKEKKKQ 479

Query: 1817 XXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1638
                              KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 480  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 539

Query: 1637 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1458
            P DITINIGKDAPIPECPIPGE WKE+R+ NTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 540  PRDITINIGKDAPIPECPIPGERWKEVRNGNTVTWLAYWNDPINQKEFKYVFLAASSTLK 599

Query: 1457 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 1281
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 600  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 659

Query: 1280 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 1101
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 660  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGS 719

Query: 1100 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 921
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG+VAE VVVYQHA
Sbjct: 720  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAENVVVYQHA 779

Query: 920  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 741
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKPP K ADGK 
Sbjct: 780  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPPTKGADGKA 839

Query: 740  KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 561
            KRNLNP+ALERKI QT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 840  KRNLNPEALERKITQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 899

Query: 560  PIEKIFNKSLLAKFAWAMDVDPGFRF 483
            PIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  PIEKIFNKSLLAKFAWSMDVDPSFRF 925


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1119 bits (2895), Expect = 0.0
 Identities = 594/928 (64%), Positives = 670/928 (72%), Gaps = 35/928 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         P++FKR           SE KK   QKH  Q      DVR
Sbjct: 1    MAVEACTPN-LMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NG+S+  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2801 SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 2634
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 179

Query: 2633 LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 2460
              ++R I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGS S +  
Sbjct: 180  SIRRRVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQQNGSVSRDGP 239

Query: 2459 -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMR------------ 2319
              K ++   KRP   VK+ ++S +KKPKLS   T   NK+A++K E              
Sbjct: 240  MNKSASLSNKRPPGEVKSLNQSSVKKPKLSSPITSISNKQAAVKPEPEPKAEDDDDNIPL 299

Query: 2318 --------------EEDDEDHVPISHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXX 2184
                          +++D+D+VPI+ R+                 +  +           
Sbjct: 300  SQRGKKSQAATSKPKDEDDDNVPIAQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKNS 359

Query: 2183 XXXXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQE 2004
                              SGEGQKWTTLVHNGVIFPPPY PHGVKMLYK +P+ LTPEQE
Sbjct: 360  KKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYNPHGVKMLYKRQPITLTPEQE 419

Query: 2003 EVATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXX 1824
            EVATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH       
Sbjct: 420  EVATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKK 479

Query: 1823 XXXXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 1644
                                KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR
Sbjct: 480  KQMTTEEKKALKEDKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKR 539

Query: 1643 IRPSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASST 1464
            IRP DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLA+W DPIN KEFKYVFLAASST
Sbjct: 540  IRPRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWIDPINQKEFKYVFLAASST 599

Query: 1463 LKGQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEK 1287
            LKGQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEK
Sbjct: 600  LKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEK 659

Query: 1286 DDDEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKK 1107
            DDDEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+
Sbjct: 660  DDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKE 719

Query: 1106 GGDDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQ 927
            G +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG+VAEKVVVYQ
Sbjct: 720  GSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAEKVVVYQ 779

Query: 926  HANKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADG 747
            HANKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+R KKGKPP K ADG
Sbjct: 780  HANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTAMEELKTDLSRVKKGKPPSKGADG 839

Query: 746  KPKRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 567
            KPKRNLNP+ALERK+AQ  AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH
Sbjct: 840  KPKRNLNPEALERKMAQINAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 899

Query: 566  EVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            EVPIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 900  EVPIEKIFNKSLLAKFAWSMDVDPSFRF 927


>dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]
          Length = 920

 Score = 1115 bits (2883), Expect = 0.0
 Identities = 593/919 (64%), Positives = 664/919 (72%), Gaps = 33/919 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         P++FKR           SE KK   QKH  Q      DVR
Sbjct: 1    MAVEACTTTNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NG+++  +K +  P +KTPPVK PL+SPK+SN+S KA  + SP  +SKPS S T  + 
Sbjct: 61   PANGENSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 120

Query: 2801 SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 2634
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 180

Query: 2633 LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 2460
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K   NGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSP 240

Query: 2459 -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 2304
              K ++   KRP   VK+ ++S LKKPKLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLSNKRPPGEVKSLNQSSLKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 2303 -----------------DHVPISHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXX 2178
                             D+VPIS R+                 +  +             
Sbjct: 301  RGKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 2177 XXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1998
                            SGEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 420

Query: 1997 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1818
            ATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQ 480

Query: 1817 XXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1638
                              KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1637 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1458
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLK 600

Query: 1457 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 1281
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 1280 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 1101
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAI QFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIHQFRSGKEGS 720

Query: 1100 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 921
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS++T GG+VAEKVVVYQHA
Sbjct: 721  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHA 780

Query: 920  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 741
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKP  K ADGKP
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKP 840

Query: 740  KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 561
            KRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 900

Query: 560  PIEKIFNKSLLAKFAWAMD 504
            PIEKIFNKSLLAKFAWAM+
Sbjct: 901  PIEKIFNKSLLAKFAWAMN 919


>dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. glabra]
          Length = 920

 Score = 1112 bits (2877), Expect = 0.0
 Identities = 592/919 (64%), Positives = 664/919 (72%), Gaps = 33/919 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         P++FKR           SE KK   Q+H  Q      DVR
Sbjct: 1    MAVEACTTTNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQRHVGQSAVHTSDVR 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NG+++  +K +  P +KTPPVK PL+SPK+SN+S KA  + S   +SKPS S T  + 
Sbjct: 61   PANGENSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSAGANSKPSASATSTAK 120

Query: 2801 SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 2634
               H        E   +    + N +S+ S+D+KPLSAR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSC 180

Query: 2633 LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 2460
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K   NGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSP 240

Query: 2459 -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 2304
              K ++   KRP   VK+ ++S LKKPKLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLSNKRPPGEVKSLNQSSLKKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 2303 -----------------DHVPISHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXX 2178
                             D+VPIS R+                 +  +             
Sbjct: 301  RGKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 2177 XXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1998
                            SGEGQKWTTLVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEV 420

Query: 1997 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1818
            ATM+AVML+TDYMNKP+FKENF +DW+K+LGKNHTIQ+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQ 480

Query: 1817 XXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1638
                              KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1637 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1458
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLAYWNDPIN KEFKYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLK 600

Query: 1457 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 1281
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 1280 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 1101
            DEADTVGCCTLKVENVEPVPPN LKFDFLGKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGS 720

Query: 1100 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 921
            +DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS++T GG+VAEKVVVYQHA
Sbjct: 721  EDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHA 780

Query: 920  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 741
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKP  K ADGKP
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKP 840

Query: 740  KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 561
            KRNLNP+ALERKIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPEALERKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 900

Query: 560  PIEKIFNKSLLAKFAWAMD 504
            PIEKIFNKSLLAKFAWAM+
Sbjct: 901  PIEKIFNKSLLAKFAWAMN 919


>dbj|BAG31374.1| topoisomerase I [Ophiorrhiza liukiuensis]
          Length = 926

 Score = 1096 bits (2834), Expect = 0.0
 Identities = 584/926 (63%), Positives = 661/926 (71%), Gaps = 33/926 (3%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVEAC    L         P++FKR           SE KK   QKH  Q      DVR
Sbjct: 1    MAVEACTTPNLMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 60

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
              NG+S+  +K +  P ++TPPVK PL+SPK+SN+S KA  + SP  +SK S S T  + 
Sbjct: 61   PANGESSSAQKGRIQPSAETPPVKSPLSSPKASNSSAKASPITSPGANSKLSASATSTAK 120

Query: 2801 SVKHQIKD----EKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTST 2634
               H        E   +    + N +S+ S+D+KPL AR     SK  SNHA K    S 
Sbjct: 121  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLIARTSGCSSKGKSNHANKEGGDSC 180

Query: 2633 LSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE-- 2460
              ++  I   EDS+DE PLS +F  KS+ GES ++S +S E+KPL +K  QNGSAS +  
Sbjct: 181  SIRRPVIEESEDSEDEKPLSRRFPSKSSLGESTSKSYDSDEKKPLATKVQQNGSASRDGP 240

Query: 2459 -RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDE------- 2304
              K ++ L KR    VK+ ++S LKK KLS   T   NK+A++K E + EDD+       
Sbjct: 241  MNKSASLLNKRAPGEVKSLNQSSLKKAKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQ 300

Query: 2303 -----------------DHVPISHRMXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXX 2178
                             D+ PIS R+                 +  +             
Sbjct: 301  RGKKSQTAASKPKDEDDDNAPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKK 360

Query: 2177 XXXXXXXXXXXXXXXXSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEV 1998
                            SGEGQKWTTLVHNGV+F PPYKPHGVKMLYK +P+ LTPEQEEV
Sbjct: 361  VIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVMFLPPYKPHGVKMLYKXQPLTLTPEQEEV 420

Query: 1997 ATMYAVMLETDYMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXX 1818
            ATM+AVML+TDYM KP+FKENF +DW+K+LGKNHT+Q+LE CDF PIYEWH         
Sbjct: 421  ATMFAVMLDTDYMTKPRFKENFFSDWKKMLGKNHTVQNLEDCDFGPIYEWHQQEKEKRKQ 480

Query: 1817 XXXXXXXXXXXXXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 1638
                              KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR
Sbjct: 481  MTTEEKKALKEERLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIR 540

Query: 1637 PSDITINIGKDAPIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLK 1458
            P DITINIGKDAPIPECPIPGE WKE+R+DNTVTWLA+WNDPIN KE KYVFLAASSTLK
Sbjct: 541  PRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWNDPINQKECKYVFLAASSTLK 600

Query: 1457 GQSDKEKYEKARLLKDYILGIRAAYTKDFTS-KDPAKRQIAVATYLIDKLALRAGNEKDD 1281
            GQSDKEKYEKARLLKDYI GIRAAYTKDFT+ KDP K+QIAVATYLIDKLALRAGNEKDD
Sbjct: 601  GQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDD 660

Query: 1280 DEADTVGCCTLKVENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGG 1101
            DEADTVGCCTLKVENVEPVPPN LK DF+GKDSIRYQNEV+VE AVFKAIQQFRSGK+G 
Sbjct: 661  DEADTVGCCTLKVENVEPVPPNILKIDFIGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGC 720

Query: 1100 DDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHA 921
             DLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLS+ET+GG VAEKV VYQHA
Sbjct: 721  ADLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGKVAEKVGVYQHA 780

Query: 920  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKP 741
            NKEVAIICNHQRTVSKSHSAQM RLNEKI+ELK  +EEL+TDL+RAKKGKPP K ADG+P
Sbjct: 781  NKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPPAKGADGEP 840

Query: 740  KRNLNPDALERKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEV 561
            KRNLNP++LERKIAQT AKIEKMERDK+TKEDLK VAL TSKI+YLDPRITVAWCKRHEV
Sbjct: 841  KRNLNPESLERKIAQTNAKIEKMERDKKTKEDLKAVALSTSKISYLDPRITVAWCKRHEV 900

Query: 560  PIEKIFNKSLLAKFAWAMDVDPGFRF 483
            PIEKIFNKSLLAKFAW+MDVDP FRF
Sbjct: 901  PIEKIFNKSLLAKFAWSMDVDPSFRF 926


>ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [Nelumbo nucifera]
          Length = 890

 Score = 1064 bits (2751), Expect = 0.0
 Identities = 572/910 (62%), Positives = 649/910 (71%), Gaps = 16/910 (1%)
 Frame = -1

Query: 3164 LMAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDV 2985
            +MAV+AC K K          P++FKR            E KK  S+K D   G+   D 
Sbjct: 1    MMAVDACIKPKFFDDGDDDEGPIIFKRNNASSKKNQLNKELKKVSSRKRDGSSGKCVSDS 60

Query: 2984 RSQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDK- 2808
             S NGQ++ ++K K V                    S KA   KS E  SK STS+TDK 
Sbjct: 61   FSPNGQNSSLQKGKAVQ-------------------SSKASTGKSTETSSKASTSITDKL 101

Query: 2807 -SNSVKHQIKD--EKLSTEATGEPNEDSQDSEDDKPLSARLP--AGLSKVNSNHAKKSPN 2643
              +  K+   D  EK   +   EP  DS DS+DDKPLS RLP  A L K  S+   K  +
Sbjct: 102  KCSPQKNSSIDNMEKQLKKPCNEPYTDSVDSDDDKPLSIRLPTSASLLKCKSSPVSKGLS 161

Query: 2642 T----STLSQKRRIP---IPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYD 2484
            +    S+ S+K  +      EDS+DE+PLS K ++K + G S  +  +SGE KPL+SK  
Sbjct: 162  SPVASSSQSEKTNVTNKKSSEDSEDELPLSLKLKLKPSVGLSGKKFYDSGEEKPLVSKLQ 221

Query: 2483 QNGSASTERKP---STNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREE 2313
            QNGS+  + +P     NL KRP  G  ++ +S +KK K+SE + P K K+ SIK+E +EE
Sbjct: 222  QNGSSKRDNEPLKSPKNLNKRPLSGANSSGQSLVKKLKVSEVSVPTKVKQTSIKSEPKEE 281

Query: 2312 DDEDHVPISHRMXXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2133
            D++D +PIS R+             K  TK +                            
Sbjct: 282  DEDD-IPISQRLKKSASSDDKSLSLKKTTKVVSSSFKKTTNKLKKAAKNSKYSKSTKMPP 340

Query: 2132 XSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNK 1953
             SGEGQKWTTL HNGVIFPP YKPHGVKMLY GKPVDLTPEQEEVATM+AVM +TDY  K
Sbjct: 341  SSGEGQKWTTLEHNGVIFPPLYKPHGVKMLYNGKPVDLTPEQEEVATMFAVMKDTDYATK 400

Query: 1952 PKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXX 1773
             +F ENFM DWR ILGKNH I+  E CDFTPIYEWH                        
Sbjct: 401  SRFIENFMNDWRTILGKNHVIKKFELCDFTPIYEWHQREKEKKKQMSAEEKKALKEEKLK 460

Query: 1772 XXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIP 1593
               KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI PSDITINIGK APIP
Sbjct: 461  QEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIHPSDITINIGKGAPIP 520

Query: 1592 ECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLK 1413
            ECPIPGE WKE+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKARLLK
Sbjct: 521  ECPIPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK 580

Query: 1412 DYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV 1233
            DYI  IRA YT+DF SKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV
Sbjct: 581  DYIKDIRANYTRDFVSKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV 640

Query: 1232 EPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHL 1053
            E VPPN L+FDFLGKDSIRY N VEVE AV+KAI QF++GK+ G+DLFD LDTSKLNAHL
Sbjct: 641  ELVPPNKLQFDFLGKDSIRYFNTVEVELAVYKAIGQFKNGKEDGEDLFDSLDTSKLNAHL 700

Query: 1052 KELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSK 873
            KELMPGLTAKVFRTYNASITLD+ML+RE++GGDVAEK+ VYQHANKEVAIICNHQR+VSK
Sbjct: 701  KELMPGLTAKVFRTYNASITLDEMLNRESKGGDVAEKIAVYQHANKEVAIICNHQRSVSK 760

Query: 872  SHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQT 693
            SHSAQM RL++KI+ELK  ++EL+TDLARAKKGKPPLK ADGKPKRNL P+ALE+KIAQ 
Sbjct: 761  SHSAQMHRLDDKIKELKDALDELKTDLARAKKGKPPLKGADGKPKRNLTPEALEKKIAQA 820

Query: 692  TAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAW 513
             AKIEKMERDK+ KEDLKTVALGTSKINYLDPRI+VAWCKRHEVPIEKIFNKSLLAKFAW
Sbjct: 821  NAKIEKMERDKQIKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAW 880

Query: 512  AMDVDPGFRF 483
            AMDVDP FRF
Sbjct: 881  AMDVDPDFRF 890


>ref|XP_023553297.1| DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo]
          Length = 887

 Score = 1060 bits (2742), Expect = 0.0
 Identities = 564/900 (62%), Positives = 650/900 (72%), Gaps = 7/900 (0%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPST-SVTDKS 2805
            S NGQS+  +K  T  L KT P++  + S K+S +S     ++ P V S  +T  V+D  
Sbjct: 60   SANGQSSNAQKRMT-SLPKTSPMRPEIGSQKASKSS-----IQEPLVKSLGTTLKVSDSM 113

Query: 2804 NSVKHQIKDEKLSTEATG-EPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 2628
            +S   Q+ +  +  E     P +D+ DSEDDKPLS RL     K NSNH  K P   T+ 
Sbjct: 114  DSRPKQLANAAVKEENDSIHPTQDNSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQ 167

Query: 2627 QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--K 2454
            + +     EDSDDE+PLSSKFQ+KS +G    R     E+KPL SK  QNGS S ++  K
Sbjct: 168  KNKIKKSSEDSDDEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQK 227

Query: 2453 PSTNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 2280
            P+    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS R
Sbjct: 228  PAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQR 287

Query: 2279 MXXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQK-WTT 2103
            M             K ++K                               SG+GQK WTT
Sbjct: 288  MKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTT 347

Query: 2102 LVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMAD 1923
            LVHNGVIFPPPY+ HGVK+L+KGKPVDLTPEQEEVATM+AVM +TDYM KPKFKENF  D
Sbjct: 348  LVHNGVIFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWND 407

Query: 1922 WRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIV 1743
            WRK+LGKNH IQ+L+ CDFTPIY+W+                           KYMWAI+
Sbjct: 408  WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAIL 467

Query: 1742 NGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWK 1563
            +GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WK
Sbjct: 468  DGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWK 527

Query: 1562 EIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAY 1383
            E+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +Y
Sbjct: 528  EVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSY 587

Query: 1382 TKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKF 1203
            TKDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F
Sbjct: 588  TKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEF 647

Query: 1202 DFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAK 1023
            +FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAK
Sbjct: 648  NFLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAK 707

Query: 1022 VFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLN 843
            VFRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLN
Sbjct: 708  VFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLN 767

Query: 842  EKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERD 663
            EKI EL+ +V+EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD
Sbjct: 768  EKISELRDIVKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERD 827

Query: 662  KETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
             +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 828  MKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_023007686.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007687.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007688.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
 ref|XP_023007689.1| DNA topoisomerase 1 beta-like [Cucurbita maxima]
          Length = 887

 Score = 1054 bits (2726), Expect = 0.0
 Identities = 560/899 (62%), Positives = 649/899 (72%), Gaps = 6/899 (0%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
            S NGQS+  +K  T  L KT P++  + S K+S +S +   +KS     K S S+  +  
Sbjct: 60   STNGQSSNAQKRMT-SLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPK 118

Query: 2801 S-VKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLSQ 2625
              V   +K+E  S      P +++ DSEDDKPLS RL     K NSNH  K P   T+ +
Sbjct: 119  QLVNAAVKEENDSIH----PAQENSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQK 168

Query: 2624 KRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--KP 2451
             +     EDSDDE+PLSSKF++KS +G    +     E+KPL SK  QNGS S+++  KP
Sbjct: 169  NKIKKSSEDSDDEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKP 228

Query: 2450 STNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHRM 2277
            +    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS RM
Sbjct: 229  AVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM 288

Query: 2276 XXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQK-WTTL 2100
                         K ++K                               SG+GQK WTTL
Sbjct: 289  KMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTL 348

Query: 2099 VHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMADW 1920
            VHNGVIFPPPY+ HGVK+L+KGKPVDLTPEQEEVATM+AVM +TDYM KPKFKENF  DW
Sbjct: 349  VHNGVIFPPPYQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDW 408

Query: 1919 RKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIVN 1740
            RK+LGKNH IQ+L+ CDFTPIY+W+                           KYMWAI++
Sbjct: 409  RKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILD 468

Query: 1739 GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWKE 1560
            GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WKE
Sbjct: 469  GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKE 528

Query: 1559 IRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAYT 1380
            +RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +YT
Sbjct: 529  VRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYT 588

Query: 1379 KDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKFD 1200
            KDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F+
Sbjct: 589  KDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFN 648

Query: 1199 FLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAKV 1020
            FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKV
Sbjct: 649  FLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKV 708

Query: 1019 FRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLNE 840
            FRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLNE
Sbjct: 709  FRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNE 768

Query: 839  KIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERDK 660
            KI EL+ +++EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD 
Sbjct: 769  KISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDM 828

Query: 659  ETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
            +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 829  KTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_022932254.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932255.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932256.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
 ref|XP_022932257.1| DNA topoisomerase 1 beta-like [Cucurbita moschata]
          Length = 887

 Score = 1051 bits (2719), Expect = 0.0
 Identities = 559/900 (62%), Positives = 649/900 (72%), Gaps = 7/900 (0%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAVE   K  +         P+VFKR            E K S SQ+ ++  GRP  D  
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNS-EVKHSQSQRLNQLSGRPGSDAC 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPST-SVTDKS 2805
            S NGQS+  +K  T  L KT P++  + S K++ +S     ++ P V S  +T  V+D  
Sbjct: 60   SANGQSSNAQKRMT-SLPKTSPMRPEIGSQKAAKSS-----IQEPLVKSLGTTLKVSDSM 113

Query: 2804 NSVKHQIKDEKLSTEATG-EPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTSTLS 2628
            +S   Q+ +  +  E     P +++ DSEDDKPLS RL     K NSNH  K P   T+ 
Sbjct: 114  DSRPKQLANAAVKEENDSIHPTQENSDSEDDKPLSIRL-----KGNSNHPSKGP-IPTVQ 167

Query: 2627 QKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTER--K 2454
            + +     EDSDDE+PLSSKFQ+KS +G    +     E+KPL SK  QNGS S ++  K
Sbjct: 168  KNKIKKSSEDSDDEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQK 227

Query: 2453 PSTNLKKRPGDGVKTTDESYL--KKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 2280
            P+    KRP     +   S +  KKPKLS+++TP      S+KA+    DD+DH+PIS R
Sbjct: 228  PAVVSSKRPLPNADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQR 287

Query: 2279 MXXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQK-WTT 2103
            M             K ++K                               SG+GQK WTT
Sbjct: 288  MKMSAAVNKSSGSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTT 347

Query: 2102 LVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMAD 1923
            LVHNGVIFPPPY+ HGVK+L+KGKPVDLT EQEEVATM+AVM +TDYM KPKFKENF  D
Sbjct: 348  LVHNGVIFPPPYQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWND 407

Query: 1922 WRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAIV 1743
            WRK+LGKNH IQ+L+ CDFTPIY+W+                           KYMWAI+
Sbjct: 408  WRKLLGKNHVIQNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAIL 467

Query: 1742 NGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESWK 1563
            +GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPG+ WK
Sbjct: 468  DGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWK 527

Query: 1562 EIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAAY 1383
            E+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IR +Y
Sbjct: 528  EVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSY 587

Query: 1382 TKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLKF 1203
            TKDFTSKDP +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+F
Sbjct: 588  TKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEF 647

Query: 1202 DFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTAK 1023
            +FLGKDSI+Y+N VEVE  VFKAI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAK
Sbjct: 648  NFLGKDSIKYENTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAK 707

Query: 1022 VFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRLN 843
            VFRTYNASITLDDML+R T+ G+V+EK+V YQHANKEVAIICNHQRTVSKSH AQM RLN
Sbjct: 708  VFRTYNASITLDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLN 767

Query: 842  EKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMERD 663
            EKI EL+ +++EL+ DL RAKKGKPPLK +DGK KRNL P+ALE+KI+QT AKIEK+ERD
Sbjct: 768  EKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERD 827

Query: 662  KETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFRF 483
             +TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDP FRF
Sbjct: 828  MKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887


>ref|XP_023748033.1| DNA topoisomerase 1 alpha-like [Lactuca sativa]
 gb|PLY62974.1| hypothetical protein LSAT_1X128740 [Lactuca sativa]
          Length = 788

 Score = 1036 bits (2680), Expect = 0.0
 Identities = 539/796 (67%), Positives = 601/796 (75%), Gaps = 10/796 (1%)
 Frame = -1

Query: 2840 DSKPSTSVTDKSNSVKHQIKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNH 2661
            D KP   +  + N+    +K     ++ + +P+ D  DSEDDKPLS R+   +SK ++NH
Sbjct: 7    DDKP---LIFRRNARPDTVKSSSQPSKPSPKPSNDEVDSEDDKPLSFRIST-VSKGDTNH 62

Query: 2660 AKKSPNTSTLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQ 2481
            AKK P TS     +     EDSDDE PLSSK+  KS  G S N        KP++ K  Q
Sbjct: 63   AKKGPGTSVSPLPKPSVKDEDSDDEKPLSSKYNSKSKVGSSNNH-------KPVVPKVHQ 115

Query: 2480 NGSASTERKPSTN--LKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDD 2307
            NGSAS +     N  L KRP D  ++     +KKPKLS++ TP  +K  ++KAE++ +DD
Sbjct: 116  NGSASKDNTQLKNIGLNKRPPDEERSAS---VKKPKLSDSVTPVNHKPVALKAEIKSDDD 172

Query: 2306 EDHVPISHRMXXXXXXXXXXXXXKNITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXS 2127
            +D   +                  ++ + +                             S
Sbjct: 173  DDDDDVPISQRIKKQAPSSSNKSSSVKQKVMKKSSSSLNQTNKKSKKMMKQKQSKYSKPS 232

Query: 2126 ------GEGQKWTTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETD 1965
                  GEGQKWT+LVH+GVIFPP YKPHGVKMLYKGKPVDLTPEQEEVATM++VML+TD
Sbjct: 233  KIPPGSGEGQKWTSLVHSGVIFPPEYKPHGVKMLYKGKPVDLTPEQEEVATMFSVMLDTD 292

Query: 1964 YMNKPKFKENFMADWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXX 1785
            YM KP+FKENF  DWRKILGKNHTIQ+LE+CDF PIYEWH                    
Sbjct: 293  YMAKPQFKENFWGDWRKILGKNHTIQNLENCDFKPIYEWHQAEKEKKKLMTTEEKKAIKE 352

Query: 1784 XXXXXXXKYMWAIVNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD 1605
                   KYMWAIV+GVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD
Sbjct: 353  EKMKIEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKD 412

Query: 1604 APIPECPIPGESWKEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKA 1425
            APIPECPIPGE WKEIRHDNTVTWLAYWNDPIN KEFKYVFLAASS+LKGQSDKEKYEKA
Sbjct: 413  APIPECPIPGERWKEIRHDNTVTWLAYWNDPINSKEFKYVFLAASSSLKGQSDKEKYEKA 472

Query: 1424 RLLKDYILGIRAAYTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 1245
            R LK YI GIR AYTKDF + D  KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLK
Sbjct: 473  RKLKGYIEGIRKAYTKDFGNSDVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLK 532

Query: 1244 VENVEPVPPNTLKFDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKL 1065
            VENVEP PPN LKFDFLGKDSIRYQNEVEVE  VFKAIQQFR+GK G DDLFD+LDTSKL
Sbjct: 533  VENVEPEPPNILKFDFLGKDSIRYQNEVEVELPVFKAIQQFRTGKAGNDDLFDKLDTSKL 592

Query: 1064 NAHLKELMPGLTAKVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQR 885
            NAHLKELMPGLTAKVFRTYNASITLDDML+RET+GGDVA+ +VVYQHANKEVAIICNHQR
Sbjct: 593  NAHLKELMPGLTAKVFRTYNASITLDDMLTRETKGGDVAQNLVVYQHANKEVAIICNHQR 652

Query: 884  TVSKSHSAQMLRLNEKIEELKGVVEELQTDLARAKKGKPP--LKSADGKPKRNLNPDALE 711
            TVSKSHSAQM RLNEKI+ELKGV++EL++DLARAKKGKPP      DGKPKRNLNP+ALE
Sbjct: 653  TVSKSHSAQMTRLNEKIDELKGVIKELESDLARAKKGKPPSNKNGPDGKPKRNLNPEALE 712

Query: 710  RKIAQTTAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSL 531
            +KIAQT AKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+FNKSL
Sbjct: 713  KKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKLFNKSL 772

Query: 530  LAKFAWAMDVDPGFRF 483
            LAKFAW+MDVDP FRF
Sbjct: 773  LAKFAWSMDVDPSFRF 788


>gb|PON81509.1| DNA topoisomerase I [Trema orientalis]
          Length = 897

 Score = 1029 bits (2660), Expect = 0.0
 Identities = 552/901 (61%), Positives = 641/901 (71%), Gaps = 8/901 (0%)
 Frame = -1

Query: 3161 MAVEACDKQKLXXXXXXXXEPLVFKRXXXXXXXXXXXSEAKKSLSQKHDRQLGRPAPDVR 2982
            MAV+A  K  L         P+VFKR           SE KK LSQK D Q GR   D  
Sbjct: 1    MAVQAPAKPNLVDDYDEDE-PIVFKRTNPISKQNQLNSEVKKPLSQKPDGQSGRQISDRV 59

Query: 2981 SQNGQSTIVKKSKTVPLSKTPPVKYPLTSPKSSNTSGKALALKSPEVDSKPSTSVTDKSN 2802
            S N  ++  +K K V  ++   VK  + SPK+S +S K   LKSP  +SK STS+  +S 
Sbjct: 60   SLNDLNSSSQKGKVVQSNRALVVKSSVASPKASASSAKTSPLKSPITNSKTSTSLDGQSK 119

Query: 2801 SVKHQ-----IKDEKLSTEATGEPNEDSQDSEDDKPLSARLPAGLSKVNSNHAKKSPNTS 2637
                +     +K+EK S +     +  S+DSEDD PLSARL    S+  S    K    S
Sbjct: 120  FSLEKNKSSIVKEEKSSIKQPIRSDSHSEDSEDDIPLSARLKGSTSQ-GSKGLVKPTTAS 178

Query: 2636 TLSQKRRIPIPEDSDDEIPLSSKFQVKSNAGESINRSSNSGERKPLLSKYDQNGSASTE- 2460
             L    +  + ED  D  PLSS+FQ+K N G S ++ ++  ++KPLLS   QNGS  T+ 
Sbjct: 179  KLPPVTKSVVKEDDSDNEPLSSRFQMKPNVGTSGSKPNDFNDKKPLLSINKQNGSTVTDV 238

Query: 2459 RKPSTNLKKRPGDGVKTTDESYLKKPKLSEAATPHKNKEASIKAEMREEDDEDHVPISHR 2280
            +KP     KRP D  K+ D+S +K+PKLS++    KNK+ S+KAE + +DD DHVPIS R
Sbjct: 239  KKPLNTNTKRPLDREKS-DQSSVKRPKLSDSPALLKNKQVSVKAESKVDDD-DHVPISQR 296

Query: 2279 MXXXXXXXXXXXXXKN-ITKPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGEGQK-WT 2106
            +             K  +TKP+                             SG+GQK WT
Sbjct: 297  IKKSPASNNKSSTTKQKVTKPVSSSFKKTTKKFKKGAKNSKYSKSTKLSPSSGDGQKKWT 356

Query: 2105 TLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMYAVMLETDYMNKPKFKENFMA 1926
            TL+HNGVIFPPPY+PHGVK+ Y GKPVDLTP QEEVATM+AVM +TDY  K  F+ENF  
Sbjct: 357  TLIHNGVIFPPPYQPHGVKLFYNGKPVDLTPVQEEVATMFAVMKDTDYAEKKTFRENFWN 416

Query: 1925 DWRKILGKNHTIQSLEHCDFTPIYEWHXXXXXXXXXXXXXXXXXXXXXXXXXXXKYMWAI 1746
            DWRK+LGKNH IQ L+ CDFTPIY+W+                           KYMWAI
Sbjct: 417  DWRKLLGKNHVIQKLDLCDFTPIYDWYQNEKEKKKQMSADEKKALKEEKLKQEEKYMWAI 476

Query: 1745 VNGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGESW 1566
            V+GVKEKVGNFRVEPPGLFRGRGEHPK GKLK+RIRPSD+TINIGKDAP+PECPIPGE W
Sbjct: 477  VDGVKEKVGNFRVEPPGLFRGRGEHPKSGKLKRRIRPSDVTINIGKDAPVPECPIPGERW 536

Query: 1565 KEIRHDNTVTWLAYWNDPINPKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYILGIRAA 1386
            KE+RHDNTVTWLA+WNDPINPKEFKYVFLAASS+LKGQSDKEKYEKAR+LKDYI  IRAA
Sbjct: 537  KEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIHNIRAA 596

Query: 1385 YTKDFTSKDPAKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNTLK 1206
            YTKDFTSKD  KRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ + PN L+
Sbjct: 597  YTKDFTSKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAIAPNKLE 656

Query: 1205 FDFLGKDSIRYQNEVEVETAVFKAIQQFRSGKKGGDDLFDRLDTSKLNAHLKELMPGLTA 1026
            F+FLGKDSIRY+N VEVE  V+KAI QF+SGKKG DDLFD LDTSKLNAHLKELMPGLTA
Sbjct: 657  FNFLGKDSIRYENTVEVELPVYKAIIQFQSGKKGNDDLFDLLDTSKLNAHLKELMPGLTA 716

Query: 1025 KVFRTYNASITLDDMLSRETRGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMLRL 846
            KVFRTYNASITLDDML++ET+ GD+ EK  VY  ANK+VAIICNHQRT+SKSH AQM +L
Sbjct: 717  KVFRTYNASITLDDMLNQETKDGDLEEKKTVYNTANKQVAIICNHQRTISKSHEAQMSKL 776

Query: 845  NEKIEELKGVVEELQTDLARAKKGKPPLKSADGKPKRNLNPDALERKIAQTTAKIEKMER 666
             EKI EL+ VV+EL+ DL RA+KGKPPLK ADGK KRNL+P+AL +KI QT  KIEK++R
Sbjct: 777  MEKIRELEDVVKELEVDLERARKGKPPLKDADGKTKRNLSPEALGKKINQTNTKIEKIKR 836

Query: 665  DKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPGFR 486
            D + KED KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAW+MDVDP FR
Sbjct: 837  DMQNKEDTKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVDPDFR 896

Query: 485  F 483
            F
Sbjct: 897  F 897