BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00036195
         (1121 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011100321.1| cytochrome P450 81D1-like [Sesamum indicum]       520   0.0  
ref|XP_012852360.1| PREDICTED: cytochrome P450 81D1-like [Erythr...   457   e-156
gb|PIN04635.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   446   e-151
ref|XP_022872978.1| isoflavone 2'-hydroxylase-like [Olea europae...   422   e-142
emb|CDP01236.1| unnamed protein product [Coffea canephora]            414   e-139
gb|PHU20730.1| hypothetical protein BC332_11881 [Capsicum chinense]   405   e-135
ref|XP_018631767.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   403   e-135
ref|XP_018631766.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   403   e-134
ref|XP_009620154.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   403   e-134
ref|XP_016568838.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   403   e-134
gb|PHT51403.1| hypothetical protein CQW23_11150 [Capsicum baccatum]   403   e-134
ref|XP_019262546.1| PREDICTED: isoflavone 2'-hydroxylase-like [N...   400   e-133
ref|XP_006362076.1| PREDICTED: cytochrome P450 81D1-like [Solanu...   400   e-133
ref|XP_009796228.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   393   e-131
gb|PHT84582.1| hypothetical protein T459_13025 [Capsicum annuum]      394   e-131
ref|XP_009796227.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   393   e-131
ref|XP_009796226.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   393   e-130
ref|XP_004238115.2| PREDICTED: cytochrome P450 81D1-like [Solanu...   400   e-130
ref|XP_015072812.1| PREDICTED: uncharacterized protein LOC107017...   401   e-128
dbj|GAV84666.1| p450 domain-containing protein [Cephalotus folli...   384   e-127

>ref|XP_011100321.1| cytochrome P450 81D1-like [Sesamum indicum]
          Length = 499

 Score =  520 bits (1340), Expect = 0.0
 Identities = 266/373 (71%), Positives = 305/373 (81%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRIS++ELLS HKLN+LS +  +
Sbjct: 102  KNDIIFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISALELLSSHKLNLLSSIPCN 161

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRLGIDDPEEAEMFREIVAEM 762
            EARTLI KLL ++N S K VDMR LFFE+ YN+V RMITG++G+++ +EAEMF++IVAEM
Sbjct: 162  EARTLILKLLENNNPS-KNVDMRKLFFEHTYNIVTRMITGKVGVENSKEAEMFQDIVAEM 220

Query: 761  AAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSGGXXX 582
            + VTLEANV DFLPF+ WFGFG+ +ER LS+VQEKR+RFMENVI+E   +EE D GG   
Sbjct: 221  SRVTLEANVADFLPFMRWFGFGN-VERKLSVVQEKRDRFMENVIEERRGEEESDGGGKQK 279

Query: 581  XXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDI 402
                   LIE L DLQR EPEYYTDETIRNL LVLVQGASHTSS TLEWAFSLLLQ+PDI
Sbjct: 280  S------LIEVLLDLQREEPEYYTDETIRNLFLVLVQGASHTSSNTLEWAFSLLLQHPDI 333

Query: 401  LTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGF 222
            LT+AR+EI         I +SD++KIPYL  IINETLRMHPAAPLLTPH SS  CIVGGF
Sbjct: 334  LTRARAEI---------IRDSDITKIPYLRGIINETLRMHPAAPLLTPHSSSRTCIVGGF 384

Query: 221  RVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENL 42
             VPRGT+LLVNAWDIQN+P+ W+DPEKF PERFEGLE GKN+FK         GCPGEN+
Sbjct: 385  HVPRGTILLVNAWDIQNDPRTWDDPEKFVPERFEGLEEGKNDFKYLPFGWGRRGCPGENM 444

Query: 41   AMHMVGLALGSLI 3
            A  MVGLALGSLI
Sbjct: 445  AKQMVGLALGSLI 457


>ref|XP_012852360.1| PREDICTED: cytochrome P450 81D1-like [Erythranthe guttata]
 gb|EYU24831.1| hypothetical protein MIMGU_mgv1a022968mg [Erythranthe guttata]
          Length = 515

 Score =  457 bits (1177), Expect = e-156
 Identities = 241/379 (63%), Positives = 290/379 (76%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +ND+VFA+RPNLLNGKHFGYNFTSLAWA YG HWRNLRRIS++ELLS  +++ L+G+RAD
Sbjct: 98   KNDVVFADRPNLLNGKHFGYNFTSLAWAPYGGHWRNLRRISTLELLSARRIHALAGVRAD 157

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRLGIDDPEEA--EMFREIVA 768
            E+R+L+QKL+   +  +  VD+R LFFE+ YN V+RMITG    ++ EE   E+F  IVA
Sbjct: 158  ESRSLVQKLIAIGD--DDDVDLRKLFFEHVYNAVMRMITGEEAAEEEEEEGWEVFGGIVA 215

Query: 767  EMAAVTLEANVVDFLPFVG-WFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSGG 591
            EM  VTLEANVVDF+P VG  FG GD +ER +S VQEKRERFMENVI++H RK++ D  G
Sbjct: 216  EMTEVTLEANVVDFVPLVGRLFGIGDCVERKMSFVQEKRERFMENVIEKHRRKKKEDEDG 275

Query: 590  XXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQN 411
                      LI+ L DLQR EPEYYTD+TIRNLLLVL+QGA+H +S TLEWAFS LLQN
Sbjct: 276  MEKS------LIQFLLDLQREEPEYYTDQTIRNLLLVLLQGATHATSATLEWAFSHLLQN 329

Query: 410  PDILTKARSEIDS-LVGEHR-LITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDEC 237
            P ILT+AR  ID+ L+G+ R LITES+V++IPYL  IINETLRMHP APLLTPHLSS EC
Sbjct: 330  PHILTRARFHIDNHLIGKERHLITESEVAQIPYLHLIINETLRMHPPAPLLTPHLSSQEC 389

Query: 236  IVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGL-EGGKNEFKXXXXXXXXXG 60
             +GGFRVPRGTMLLVNAWDI NNP+ W +P +F PERFEG+ +  KN F          G
Sbjct: 390  TIGGFRVPRGTMLLVNAWDIHNNPETWENPNEFLPERFEGVGKNNKNGFDFLPFGWGRRG 449

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGE LA+ M+G  LG LI
Sbjct: 450  CPGEKLAVAMIGAVLGCLI 468


>gb|PIN04635.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 485

 Score =  446 bits (1146), Expect = e-151
 Identities = 235/378 (62%), Positives = 282/378 (74%), Gaps = 5/378 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            QNDI+FANRP LLNGKHFGYNFTSLAW+SYGDHWRNLRRIS++ LLS HKL +LS +R +
Sbjct: 96   QNDIIFANRPPLLNGKHFGYNFTSLAWSSYGDHWRNLRRISAIGLLSSHKLQMLSSIRVN 155

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL-GIDDPEEAEMFREIVAE 765
            E+R +I ++++D+   N  +DMRA FF Y YNVV+ MITG L G++D   +EMF+EIV E
Sbjct: 156  ESRAMITRMIKDN--KNDPIDMRATFFGYTYNVVMGMITGELQGVED--SSEMFQEIVEE 211

Query: 764  MAAVTLEANVVDFLPFVG-WFGFGDYIERNLSLVQEKRERFMENVIKEH--YRKEEGDSG 594
            M+ VTLEAN+VDFLPFVG WFGF   IER  S +QEKR++FMENVI++H    +E GD+G
Sbjct: 212  MSRVTLEANIVDFLPFVGYWFGFKADIERKFSSIQEKRDKFMENVIEKHRGILEEIGDNG 271

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K LI+ L DL+R +PEYYTDETIRNLLLVLVQGASH++S TLEWAFSLLL+
Sbjct: 272  ------HDQKSLIKVLLDLERGQPEYYTDETIRNLLLVLVQGASHSTSNTLEWAFSLLLE 325

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NPD +T+AR+EID+LVG+                    +TLRMHPAAPLLTPH SS++CI
Sbjct: 326  NPDTMTRARAEIDNLVGK-------------------EQTLRMHPAAPLLTPHYSSEKCI 366

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKN-EFKXXXXXXXXXGC 57
            VGGF VP GTMLLVN WD QNNPQ W DPEKF P+RF G    K+  FK          C
Sbjct: 367  VGGFSVPPGTMLLVNVWDFQNNPQTWKDPEKFMPDRFTGKNSYKDYNFKFVPFGRGRRAC 426

Query: 56   PGENLAMHMVGLALGSLI 3
            PGENLAM+MVGLALG+LI
Sbjct: 427  PGENLAMNMVGLALGTLI 444


>ref|XP_022872978.1| isoflavone 2'-hydroxylase-like [Olea europaea var. sylvestris]
          Length = 500

 Score =  422 bits (1085), Expect = e-142
 Identities = 219/377 (58%), Positives = 277/377 (73%), Gaps = 4/377 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRPNLLNGK FGYN+TSLAW+ YGDHWRNLRRISS+ LLS HK+ + S ++AD
Sbjct: 92   KNDIIFANRPNLLNGKVFGYNYTSLAWSPYGDHWRNLRRISSLHLLSSHKVKMNSNIQAD 151

Query: 941  EARTLIQKLLRDHNVS-NKGVDMRALFFEYAYNVVVRMITGR---LGIDDPEEAEMFREI 774
            EAR LI+K +R  N + NK V+ + + FE+ +NV+ RMITG+   LG      AE+FREI
Sbjct: 152  EARILIRKFVRFANDNPNKAVEFKWILFEFMFNVITRMITGKNYKLG------AEIFREI 205

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
             +E++ VT+EANV DFLPF+ WFGF +  E+ + L+Q KR++FM+N+I+EH      D  
Sbjct: 206  TSEISRVTMEANVEDFLPFMKWFGFKN-TEKKMKLIQMKRDKFMQNIIEEHRSIVLEDDN 264

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K  ++ L DLQR EPEYY+DETIRNLLLVLV GA+HT+S T+EWAFS LL 
Sbjct: 265  ------VKEKSTLQILLDLQREEPEYYSDETIRNLLLVLVHGATHTTSVTMEWAFSHLLD 318

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NP IL +A+ +ID+LVG+ RLI ESD++++PYL  I+NETLR+HP  PLL PH+SS EC 
Sbjct: 319  NPYILRRAQDDIDTLVGKERLIDESDIAELPYLRCIVNETLRLHPPLPLLLPHVSSGECT 378

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCP 54
            VGGFRVP GT+L VN W +QN+ + W D EKFKPERFEG   GK+E K          CP
Sbjct: 379  VGGFRVPLGTLLFVNVWAVQNDDEKWVDAEKFKPERFEGFR-GKDELKFFPFGWGRRACP 437

Query: 53   GENLAMHMVGLALGSLI 3
            GENLAM +V LALGSLI
Sbjct: 438  GENLAMRVVELALGSLI 454


>emb|CDP01236.1| unnamed protein product [Coffea canephora]
          Length = 511

 Score =  414 bits (1065), Expect = e-139
 Identities = 209/378 (55%), Positives = 276/378 (73%), Gaps = 5/378 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRPN LNGK+FGYN+TSL W+SYG+HWRNLR+ISS+E+LS HKL +LS +RAD
Sbjct: 89   KNDIIFANRPNFLNGKYFGYNYTSLPWSSYGEHWRNLRKISSLEVLSSHKLQLLSDIRAD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E  +LI+KL R      ++  DMR+  F+  +NV+ RMITG       I++ EEA +F E
Sbjct: 149  EVLSLIRKLFRVSVEDPDQFADMRSACFDLTFNVMTRMITGNRYYGEDIENSEEARIFHE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDS 597
            I +E A V  +ANV+DFLPF+ WFG  D +E+ +S +QE+R++FM+NVI EH R   G S
Sbjct: 209  ITSEAAKVNPKANVLDFLPFMRWFGLRDVVEK-MSELQERRDKFMQNVIDEH-RARAGAS 266

Query: 596  GGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLL 417
            G         + +I AL DL   +PEYY++ETIRNL+LVL+Q  S T+++T+EWA + LL
Sbjct: 267  G-------EKRTMIGALLDLHEKDPEYYSEETIRNLMLVLLQAGSDTTASTMEWAMAYLL 319

Query: 416  QNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDEC 237
             NP++L KA++EID  VG  R I E D+ ++PYL  IINETLR+ P  PLL PH +S +C
Sbjct: 320  DNPEVLKKAQAEIDQEVGHGRFIGECDLPQLPYLHCIINETLRLQPVTPLLMPHKASQDC 379

Query: 236  IVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGC 57
            IVGG+RVPRGT+L VNAWDIQ+N +YW+DP+KF P+RFE L GGK EFK         GC
Sbjct: 380  IVGGYRVPRGTVLFVNAWDIQHNSRYWDDPDKFMPQRFEALVGGKEEFKFIPFGSGRRGC 439

Query: 56   PGENLAMHMVGLALGSLI 3
            PG NLA+H++GLALGSL+
Sbjct: 440  PGNNLAIHVLGLALGSLL 457


>gb|PHU20730.1| hypothetical protein BC332_11881 [Capsicum chinense]
          Length = 506

 Score =  405 bits (1042), Expect = e-135
 Identities = 204/377 (54%), Positives = 274/377 (72%), Gaps = 4/377 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+ S +++  LSG+R+D
Sbjct: 88   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVFSSYRIQTLSGIRSD 147

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFREI 774
            E   LI++L  +   S K V+M++  F + +NV+ RMI G+      +++ +EA++F++I
Sbjct: 148  EINYLIRRLSMES--SEKFVEMKSSLFNFTFNVISRMIAGKRYYGEKVENSKEAKLFQDI 205

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
                     +AN++DFLPF+ WFG    +E  +  +QEKR++FM+N I EH R +   SG
Sbjct: 206  SKATTTTIPKANILDFLPFMRWFGLHS-VEEKMRELQEKRDKFMQNEIDEHRRLKT--SG 262

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL 
Sbjct: 263  SAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLD 322

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NP+IL KA++EID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC 
Sbjct: 323  NPEILKKAQTEIDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECK 382

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCP 54
            V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCP
Sbjct: 383  VAGYRVPRGTVLLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCP 442

Query: 53   GENLAMHMVGLALGSLI 3
            GENLA+H++GLALGSL+
Sbjct: 443  GENLAIHVIGLALGSLL 459


>ref|XP_018631767.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X3 [Nicotiana
            tomentosiformis]
          Length = 494

 Score =  403 bits (1036), Expect = e-135
 Identities = 205/379 (54%), Positives = 272/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLYRVSMESSEKIVEMKSSLFNFTFNVISRMIAGKRFYGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ WFG  + +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTIPKANILDFLPFMRWFGLHN-VEEKMMKVQEKRDNFMQKEIQEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNLLLVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLLLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSKE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWEDPEKFNPDRFIGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_018631766.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
            tomentosiformis]
          Length = 508

 Score =  403 bits (1036), Expect = e-134
 Identities = 205/379 (54%), Positives = 272/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLYRVSMESSEKIVEMKSSLFNFTFNVISRMIAGKRFYGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ WFG  + +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTIPKANILDFLPFMRWFGLHN-VEEKMMKVQEKRDNFMQKEIQEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNLLLVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLLLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSKE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWEDPEKFNPDRFIGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_009620154.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
            tomentosiformis]
 ref|XP_016477062.1| PREDICTED: isoflavone 2'-hydroxylase-like [Nicotiana tabacum]
          Length = 513

 Score =  403 bits (1036), Expect = e-134
 Identities = 205/379 (54%), Positives = 272/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLYRVSMESSEKIVEMKSSLFNFTFNVISRMIAGKRFYGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ WFG  + +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTIPKANILDFLPFMRWFGLHN-VEEKMMKVQEKRDNFMQKEIQEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNLLLVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLLLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSKE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWEDPEKFNPDRFIGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_016568838.1| PREDICTED: isoflavone 2'-hydroxylase-like [Capsicum annuum]
          Length = 506

 Score =  403 bits (1035), Expect = e-134
 Identities = 203/377 (53%), Positives = 273/377 (72%), Gaps = 4/377 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+ S +++  LSG+R+D
Sbjct: 88   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVFSSYRIQTLSGIRSD 147

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFREI 774
            E   LI++L  +   S K V+M++  F + +NV+ RMI G+      +++ +EA++F++I
Sbjct: 148  EINYLIRRLSMES--SEKIVEMKSSLFNFTFNVISRMIAGKRYYGEKVENSKEAKLFQDI 205

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
                     +AN++DFLPF+ WFG    +E  +  +QEKR++FM+  I EH R +   SG
Sbjct: 206  SKATTTTIPKANILDFLPFMRWFGLHS-VEEKMRELQEKRDKFMQKEIDEHRRLKT--SG 262

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL 
Sbjct: 263  SAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLD 322

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NP+IL KA++EID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC 
Sbjct: 323  NPEILKKAQTEIDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECK 382

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCP 54
            V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCP
Sbjct: 383  VAGYRVPRGTVLLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCP 442

Query: 53   GENLAMHMVGLALGSLI 3
            GENLA+H++GLALGSL+
Sbjct: 443  GENLAIHVIGLALGSLL 459


>gb|PHT51403.1| hypothetical protein CQW23_11150 [Capsicum baccatum]
          Length = 507

 Score =  403 bits (1035), Expect = e-134
 Identities = 203/377 (53%), Positives = 273/377 (72%), Gaps = 4/377 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+ S +++  LSG+R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVFSSYRIQTLSGIRSD 148

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFREI 774
            E   LI++L  +   S K V+M++  F + +NV+ RMI G+      +++ +EA++F++I
Sbjct: 149  EINYLIRRLSMES--SEKIVEMKSSLFNFTFNVISRMIAGKRYYGEKVENSKEAKLFQDI 206

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
                     +AN++DFLPF+ WFG    +E  +  +QEKR++FM+  I EH R +   SG
Sbjct: 207  SKATTTTIPKANILDFLPFMRWFGLHS-VEEKMRELQEKRDKFMQKEIDEHRRLKT--SG 263

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL 
Sbjct: 264  SAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLD 323

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NP+IL KA++EID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC 
Sbjct: 324  NPEILRKAQTEIDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECK 383

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCP 54
            V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCP
Sbjct: 384  VAGYRVPRGTVLLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCP 443

Query: 53   GENLAMHMVGLALGSLI 3
            GENLA+H++GLALGSL+
Sbjct: 444  GENLAIHIIGLALGSLL 460


>ref|XP_019262546.1| PREDICTED: isoflavone 2'-hydroxylase-like [Nicotiana attenuata]
 gb|OIT37725.1| cytochrome p450 81d1 [Nicotiana attenuata]
          Length = 513

 Score =  400 bits (1027), Expect = e-133
 Identities = 202/379 (53%), Positives = 272/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLRDHNVSNKG-VDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R    S++  V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLFRVSIESSENIVEMKSSLFNFTFNVISRMIAGKRYYGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ WFG    +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTAPKANILDFLPFMRWFGLHS-VEEKMMEVQEKRDNFMKKEIEEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +P YYTDETIRNL+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPGYYTDETIRNLMLVLLQAGSETSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS+E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+R+PRGT+LLVNAW I ++P+ W DPEKF PERF G EG K   K         G
Sbjct: 388  CKVAGYRIPRGTVLLVNAWGIHHDPKVWEDPEKFNPERFIGFEGIKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_006362076.1| PREDICTED: cytochrome P450 81D1-like [Solanum tuberosum]
          Length = 513

 Score =  400 bits (1027), Expect = e-133
 Identities = 203/379 (53%), Positives = 272/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GKHFGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKHFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V+M++  F + +NV+ RMI G+      +++ +EA++F++
Sbjct: 149  EINYLIRRLFRVSIESSEKIVEMKSSLFNFTFNVLSRMIAGKRYYGEKVENSKEAKLFQD 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I         +AN++DFLPF+ WFG  D  E+ + L Q+KR+ FM+  I EH R K  G 
Sbjct: 209  ISKATIITIPKANILDFLPFMRWFGLHDVEEKMMEL-QKKRDNFMQKEIDEHRRLKTNGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIR+L+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRSLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L NP+IL  A++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNPEILKTAQTEIDNQVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W +P+KF P RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWEEPQKFNPGRFLGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA+H++GLALGSL+
Sbjct: 448  CPGENLAIHVIGLALGSLL 466


>ref|XP_009796228.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X3 [Nicotiana
            sylvestris]
 ref|XP_016503272.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X3 [Nicotiana
            tabacum]
          Length = 494

 Score =  393 bits (1010), Expect = e-131
 Identities = 201/379 (53%), Positives = 271/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLRDHNVSNKG-VDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R    S++  V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLFRVSIESSENIVEMKSSIFNFTFNVISRMIAGKRYHGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ W G    +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTVPKANILDFLPFMRWCGLHS-VEEKMMEVQEKRDNFMKKEIEEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++ Y+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLHYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V  +RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CNVASYRVPRGTVLLVNAWGIHHDPKVWEDPEKFYPDRFIGFEGIKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>gb|PHT84582.1| hypothetical protein T459_13025 [Capsicum annuum]
          Length = 515

 Score =  394 bits (1011), Expect = e-131
 Identities = 202/386 (52%), Positives = 272/386 (70%), Gaps = 13/386 (3%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+ S +++  LSG+R+D
Sbjct: 88   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVFSSYRIQTLSGIRSD 147

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFREI 774
            E   LI++L  +   S K V+M++  F + +NV+ RMI G+      +++ +EA++F++I
Sbjct: 148  EINYLIRRLSMES--SEKIVEMKSSLFNFTFNVISRMIAGKRYYGEKVENSKEAKLFQDI 205

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
                     +AN++DFLPF+ WFG    +E  +  +QEKR++FM+  I EH R +   SG
Sbjct: 206  SKATTTTIPKANILDFLPFMRWFGLHS-VEEKMRELQEKRDKFMQKEIDEHRRLKT--SG 262

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLL---------VLVQGASHTSSTTL 441
                     K ++E L DLQ+ +PEYYTDETIRNL+          VL+Q  S TS+ TL
Sbjct: 263  SAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLIFIHTIFLCMQVLLQAGSDTSAVTL 322

Query: 440  EWAFSLLLQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLT 261
            EWAFS LL NP+IL KA++EID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL 
Sbjct: 323  EWAFSHLLDNPEILKKAQTEIDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLV 382

Query: 260  PHLSSDECIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXX 81
            PHLSS+EC V G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G E  K   K   
Sbjct: 383  PHLSSEECKVAGYRVPRGTVLLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIP 442

Query: 80   XXXXXXGCPGENLAMHMVGLALGSLI 3
                  GCPGENLA+H++GLALGSL+
Sbjct: 443  FGSGRRGCPGENLAIHVIGLALGSLL 468


>ref|XP_009796227.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
            sylvestris]
 ref|XP_016503271.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
            tabacum]
          Length = 508

 Score =  393 bits (1010), Expect = e-131
 Identities = 201/379 (53%), Positives = 271/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLRDHNVSNKG-VDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R    S++  V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLFRVSIESSENIVEMKSSIFNFTFNVISRMIAGKRYHGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ W G    +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTVPKANILDFLPFMRWCGLHS-VEEKMMEVQEKRDNFMKKEIEEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++ Y+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLHYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V  +RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CNVASYRVPRGTVLLVNAWGIHHDPKVWEDPEKFYPDRFIGFEGIKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_009796226.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
            sylvestris]
 ref|XP_016503270.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
            tabacum]
          Length = 513

 Score =  393 bits (1010), Expect = e-130
 Identities = 201/379 (53%), Positives = 271/379 (71%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISSLEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLRDHNVSNKG-VDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R    S++  V+M++  F + +NV+ RMI G+      +++ +EA++F+E
Sbjct: 149  EINYLIRRLFRVSIESSENIVEMKSSIFNFTFNVISRMIAGKRYHGEKVENSKEAKLFQE 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I  +      +AN++DFLPF+ W G    +E  +  VQEKR+ FM+  I+EH + K  G 
Sbjct: 209  ISKDTLTTVPKANILDFLPFMRWCGLHS-VEEKMMEVQEKRDNFMKKEIEEHRQLKTSGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L N +IL KA++EID+ VG+ RLI ESD++++ Y+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNLEILKKAQAEIDNHVGQDRLIDESDLAQLHYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V  +RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G EG K   K         G
Sbjct: 388  CNVASYRVPRGTVLLVNAWGIHHDPKVWEDPEKFYPDRFIGFEGIKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA H++GLALGSL+
Sbjct: 448  CPGENLAFHVIGLALGSLL 466


>ref|XP_004238115.2| PREDICTED: cytochrome P450 81D1-like [Solanum lycopersicum]
          Length = 755

 Score =  400 bits (1029), Expect = e-130
 Identities = 202/379 (53%), Positives = 273/379 (72%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GKHFGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKHFGYNFTSLAWSSYGEHWRNLRRISSIEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V++++  F + +NV+ RMI G+      +++ +EA++F++
Sbjct: 149  EINYLIRRLFRVSIEGSEKIVEIKSSLFNFTFNVLSRMIAGKRYYGEKVENSKEAKLFQD 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I         +AN++DFLPF+ WFG  D  E+ + L Q+KR+ FM+  I EH R K  G 
Sbjct: 209  ISKATITTIPKANILDFLPFMRWFGLHDVEEKMMEL-QKKRDNFMQKEIDEHRRLKTNGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIR+L+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKIIMEVLLDLQKTDPEYYTDETIRSLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L NP+IL  A++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNPEILKTAQTEIDNKVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W +P+KF P+RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWKEPQKFNPDRFLGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA+H++GLALGSL+
Sbjct: 448  CPGENLAIHVIGLALGSLL 466



 Score =  235 bits (600), Expect = 3e-67
 Identities = 113/180 (62%), Positives = 141/180 (78%)
 Frame = -1

Query: 542  DLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSEIDSLVG 363
            DLQ+ +P+YYTD+ IRNLLL+L+Q  S TS+ TLEWAFS LL NP+IL  A++EID+ VG
Sbjct: 540  DLQKTDPKYYTDKMIRNLLLILLQAGSDTSAITLEWAFSRLLDNPEILKTAQTEIDNQVG 599

Query: 362  EHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFRVPRGTMLLVNAW 183
            + RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+EC V G+RVPRGT+LLVNAW
Sbjct: 600  QDRLIDESDLAQLPYIRCIINETLRMHPAAPLLLPHLSSEECKVAGYRVPRGTILLVNAW 659

Query: 182  DIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGLALGSLI 3
             I ++P+ W DPEKF P+RF G E  K   K         GCPGENLA+H++GLALGSL+
Sbjct: 660  GIHHDPKVWEDPEKFNPDRFIGFESVKEGCKFIPFGSGRRGCPGENLAIHVIGLALGSLL 719


>ref|XP_015072812.1| PREDICTED: uncharacterized protein LOC107017045 [Solanum pennellii]
          Length = 1013

 Score =  401 bits (1030), Expect = e-128
 Identities = 202/379 (53%), Positives = 273/379 (72%), Gaps = 6/379 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GKHFGYNFTSLAW+SYG+HWRNLRRISS+E+LS +++  LS +R+D
Sbjct: 89   KNDIIFANRPDFLSGKHFGYNFTSLAWSSYGEHWRNLRRISSIEVLSSYRIQTLSSIRSD 148

Query: 941  EARTLIQKLLR-DHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFRE 777
            E   LI++L R     S K V++++  F + +NV+ RMI G+      +++ +EA++F++
Sbjct: 149  EINYLIRRLFRVSIESSEKIVEIKSSLFNFTFNVLSRMIAGKRYYGEKVENSKEAKLFQD 208

Query: 776  IVAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGD 600
            I         +AN++DFLPF+ WFG  D  E+ + L Q+KR+ FM+  I EH R K  G 
Sbjct: 209  ISKATITTIPKANILDFLPFMRWFGLHDVEEKMMEL-QKKRDNFMQKEIDEHRRLKTNGS 267

Query: 599  SGGXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLL 420
                       K ++E L DLQ+ +PEYYTDETIR+L+LVL+Q  S TS+ TLEWAFS L
Sbjct: 268  FPSAEVVAGKKKTIMEVLLDLQKTDPEYYTDETIRSLMLVLLQAGSDTSAVTLEWAFSHL 327

Query: 419  LQNPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDE 240
            L NP+IL  A++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+E
Sbjct: 328  LDNPEILKTAQTEIDNKVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHLSSEE 387

Query: 239  CIVGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXG 60
            C V G+RVPRGT+LLVNAW I ++P+ W +P+KF P+RF G EG K   K         G
Sbjct: 388  CKVAGYRVPRGTVLLVNAWGIHHDPKVWKEPQKFNPDRFLGFEGVKEGCKFIPFGSGRRG 447

Query: 59   CPGENLAMHMVGLALGSLI 3
            CPGENLA+H++GLALGSL+
Sbjct: 448  CPGENLAIHVIGLALGSLL 466



 Score =  382 bits (981), Expect = e-121
 Identities = 197/376 (52%), Positives = 267/376 (71%), Gaps = 3/376 (0%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +NDI+FANRP+ L+GK+FGYNFTSLAW+SYG+HWRNLRRIS++E+LS +++  LS +R+D
Sbjct: 592  KNDIIFANRPDFLSGKYFGYNFTSLAWSSYGEHWRNLRRISTLEVLSSYRIQTLSIIRSD 651

Query: 941  EARTLIQKLLRDHNVSNKG-VDMRALFFEYAYNVVVRMITG-RLGIDDPEEAEMFREIVA 768
            E   LI++L R    S++  V+M++  F + +NV+ RMI G R    + +EA +F++I  
Sbjct: 652  EINYLIRRLFRVSIESSEIIVEMKSSLFNFTFNVISRMIAGKRYYGGNSKEAMLFQDISK 711

Query: 767  EMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYR-KEEGDSGG 591
            +      +AN++DFLPF+ WFG     E+ + L Q+KR+ FM   I EH R K  G    
Sbjct: 712  DTINTAPKANILDFLPFMRWFGLHSVEEKMMEL-QKKRDNFMRKEIDEHSRLKSSGSFPS 770

Query: 590  XXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQN 411
                    K ++E L DLQ+ +P+YYTD+ IRNLLL+L+Q  S TS+ TLEWAFS LL N
Sbjct: 771  AEVVAGKKKTIMEVLLDLQKTDPKYYTDKMIRNLLLILLQAGSDTSAITLEWAFSRLLDN 830

Query: 410  PDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIV 231
            P+IL  A++EID+ VG+ RLI ESD++++PY+  IINETLRMHPAA LL PHLSS+EC V
Sbjct: 831  PEILKTAQTEIDNQVGQDRLIDESDLAQLPYIRCIINETLRMHPAARLLLPHLSSEECKV 890

Query: 230  GGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPG 51
             G+RVPRGT+LLVNAW I ++P+ W DPEKF P+RF G +  K   K         GCPG
Sbjct: 891  SGYRVPRGTILLVNAWGIHHDPKAWEDPEKFNPDRFIGFDSVKEGCKFIPFGSGRRGCPG 950

Query: 50   ENLAMHMVGLALGSLI 3
            ENLA+H++GLALGSL+
Sbjct: 951  ENLAIHVIGLALGSLL 966


>dbj|GAV84666.1| p450 domain-containing protein [Cephalotus follicularis]
          Length = 503

 Score =  384 bits (986), Expect = e-127
 Identities = 200/377 (53%), Positives = 259/377 (68%), Gaps = 4/377 (1%)
 Frame = -1

Query: 1121 QNDIVFANRPNLLNGKHFGYNFTSLAWASYGDHWRNLRRISSVELLSFHKLNILSGLRAD 942
            +ND VFANRP LL GK+ G N+T+L+WA YGD+WRNLRRIS +ELLS  +L +LS LRAD
Sbjct: 85   KNDTVFANRPRLLAGKYIGNNYTTLSWAPYGDNWRNLRRISLLELLSTSRLQMLSSLRAD 144

Query: 941  EARTLIQKLLRDHNVSNKGVDMRALFFEYAYNVVVRMITGRL----GIDDPEEAEMFREI 774
            E R++I +L    N +   VDM++ FFE   NV++RMI G+      ++D EEA+ FRE 
Sbjct: 145  EVRSMIHQLFITRNQT---VDMKSTFFELTLNVMMRMIAGKRYYGDNVEDVEEAKKFRER 201

Query: 773  VAEMAAVTLEANVVDFLPFVGWFGFGDYIERNLSLVQEKRERFMENVIKEHYRKEEGDSG 594
            V E   V    N+VDFLP + WFG G  +E+ L L+++KR++FM+++I+++ R    D  
Sbjct: 202  VEETFLVAGVTNMVDFLPILRWFGIGG-MEKKLMLLKQKRDKFMQDLIEDNRRVMGSDDD 260

Query: 593  GXXXXXXXXKCLIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQ 414
                     K +I+ L  LQ  EP+YY DETI NL+ VL+   + TS+ T+EWA SLLL 
Sbjct: 261  SSLLGGGRKKTMIQVLLSLQETEPQYYKDETIGNLMGVLLGAGTDTSAGTMEWAMSLLLN 320

Query: 413  NPDILTKARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECI 234
            NP +L K+++EID+ VG  RLI E D++K+PYL  IINETLRM+PA PLL PH SS EC+
Sbjct: 321  NPRVLKKSQTEIDTHVGHDRLINEGDLAKLPYLHCIINETLRMYPAGPLLVPHESSKECV 380

Query: 233  VGGFRVPRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCP 54
            VG FRVPRGTMLLVN W I N+P+ W +P +FKPERFEGLEG K+ FK          CP
Sbjct: 381  VGNFRVPRGTMLLVNMWAIHNDPKIWEEPREFKPERFEGLEGWKDGFKLMAFGTGRRSCP 440

Query: 53   GENLAMHMVGLALGSLI 3
            GE LA  MVGLAL SLI
Sbjct: 441  GEGLATRMVGLALASLI 457