BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00034270
         (584 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN03953.1| hypothetical protein CDL12_23518 [Handroanthus im...   105   7e-24
gb|PIN25894.1| hypothetical protein CDL12_01363 [Handroanthus im...    96   3e-20
gb|PIN26205.1| hypothetical protein CDL12_01037 [Handroanthus im...    96   3e-20
ref|XP_011093817.1| growth-regulating factor 1 isoform X1 [Sesam...    94   4e-19
ref|XP_009596803.1| PREDICTED: growth-regulating factor 1-like [...    87   9e-17
ref|XP_011078072.1| growth-regulating factor 1 [Sesamum indicum]       84   3e-16
ref|XP_022883235.1| growth-regulating factor 6-like, partial [Ol...    84   3e-16
ref|XP_009767378.1| PREDICTED: growth-regulating factor 1-like [...    80   3e-14
ref|XP_016449439.1| PREDICTED: growth-regulating factor 1-like i...    80   3e-14
ref|XP_004243717.1| PREDICTED: growth-regulating factor 1-like [...    77   3e-13
ref|XP_006342364.1| PREDICTED: growth-regulating factor 1 [Solan...    76   8e-13
gb|PHT41787.1| hypothetical protein CQW23_20641 [Capsicum baccatum]    76   1e-12
ref|XP_015081067.1| PREDICTED: growth-regulating factor 5-like [...    74   4e-12
ref|XP_023744379.1| growth-regulating factor 1 isoform X2 [Lactu...    73   7e-12
ref|XP_023744378.1| growth-regulating factor 1 isoform X1 [Lactu...    73   8e-12
ref|XP_016539546.1| PREDICTED: LOW QUALITY PROTEIN: growth-regul...    73   8e-12
gb|PHU10440.1| Growth-regulating factor 5, partial [Capsicum chi...    73   9e-12
gb|PHT62641.1| hypothetical protein T459_33536, partial [Capsicu...    73   9e-12
ref|XP_019225282.1| PREDICTED: growth-regulating factor 1-like [...    72   3e-11
ref|XP_021996440.1| growth-regulating factor 1-like isoform X3 [...    69   2e-10

>gb|PIN03953.1| hypothetical protein CDL12_23518 [Handroanthus impetiginosus]
          Length = 336

 Score =  105 bits (263), Expect = 7e-24
 Identities = 67/108 (62%), Positives = 73/108 (67%), Gaps = 10/108 (9%)
 Frame = +2

Query: 5   WQLAPLTMGFPL---KDKAYNSSCLENG--LQXXXXXXXXYNSDINPLNIERDDDQ-PKK 166
           WQLAPLTMG  L   KDKAY+SS L +G   +        YN ++ PLNIERDD    KK
Sbjct: 231 WQLAPLTMGSSLSQMKDKAYSSSRLGSGHHQKINHEHQHYYNCEV-PLNIERDDHHHSKK 289

Query: 167 VMHHFLDEWPPKD----PISEDKLSISNRTQLSISITNSLHDFFMTPK 298
           VMHHFL+EW PKD    P SEDKLS S  TQLSISI NSLHDFFMT K
Sbjct: 290 VMHHFLEEWSPKDKDSWPNSEDKLS-SQSTQLSISIPNSLHDFFMTQK 336


>gb|PIN25894.1| hypothetical protein CDL12_01363 [Handroanthus impetiginosus]
          Length = 320

 Score = 95.9 bits (237), Expect = 3e-20
 Identities = 59/103 (57%), Positives = 67/103 (65%), Gaps = 4/103 (3%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKVMHHF 181
           SW+L   +    +K  AY+S  L++           Y SD+ P NIERD DQPKKV HHF
Sbjct: 221 SWKLTMGSSLSQIKPNAYSSD-LQSRQWNESLNGHYYPSDM-PSNIERDIDQPKKVTHHF 278

Query: 182 LDEWPPKD----PISEDKLSISNRTQLSISITNSLHDFFMTPK 298
           LDEW PKD    P SE+KLS SNRTQLSISI NSLHDFFMT K
Sbjct: 279 LDEWEPKDKDSWPSSENKLS-SNRTQLSISIPNSLHDFFMTQK 320


>gb|PIN26205.1| hypothetical protein CDL12_01037 [Handroanthus impetiginosus]
          Length = 321

 Score = 95.9 bits (237), Expect = 3e-20
 Identities = 59/103 (57%), Positives = 67/103 (65%), Gaps = 4/103 (3%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKVMHHF 181
           SW+L   +    +K  AY+S  L++           Y SD+ P NIERD DQPKKV HHF
Sbjct: 221 SWKLTMGSSLSQIKPNAYSSD-LQSRQWNESLNGHYYPSDM-PSNIERDIDQPKKVTHHF 278

Query: 182 LDEWPPKD----PISEDKLSISNRTQLSISITNSLHDFFMTPK 298
           LDEW PKD    P SE+KLS SNRTQLSISI NSLHDFFMT K
Sbjct: 279 LDEWEPKDKDSWPSSENKLS-SNRTQLSISIPNSLHDFFMTQK 320


>ref|XP_011093817.1| growth-regulating factor 1 isoform X1 [Sesamum indicum]
 ref|XP_020553366.1| growth-regulating factor 1 isoform X2 [Sesamum indicum]
          Length = 357

 Score = 93.6 bits (231), Expect = 4e-19
 Identities = 58/114 (50%), Positives = 71/114 (62%), Gaps = 15/114 (13%)
 Frame = +2

Query: 2   SWQLAPLTMGFP----LKDKAYNSSCLENGL-------QXXXXXXXXYNSDINPLNIERD 148
           SW+LAPLT+G      +K  A  SS  +NG        Q        Y++    LN+ERD
Sbjct: 246 SWKLAPLTVGSSSLPQMKHNANYSSDFQNGQRIQSLNSQQKHHDHYYYSTCDMALNLERD 305

Query: 149 DDQPKKVMHHFLDEWPPKDP----ISEDKLSISNRTQLSISITNSLHDFFMTPK 298
           D QPKKVMHHFLDEWPP+D      SEDK + +++TQLSIS  NS+HDFFMTPK
Sbjct: 306 D-QPKKVMHHFLDEWPPRDKDSWLTSEDK-TPTHKTQLSISTPNSMHDFFMTPK 357


>ref|XP_009596803.1| PREDICTED: growth-regulating factor 1-like [Nicotiana
           tomentosiformis]
 ref|XP_016497921.1| PREDICTED: growth-regulating factor 1-like [Nicotiana tabacum]
          Length = 361

 Score = 87.0 bits (214), Expect = 9e-17
 Identities = 50/111 (45%), Positives = 62/111 (55%), Gaps = 14/111 (12%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYN---------SSCLE-NGLQXXXXXXXXYNSDINPLNIERDD 151
           +WQL+PLTMG P+K + Y+         SS L+   L         Y  D+  + IE   
Sbjct: 247 TWQLSPLTMGSPMKQRTYSLSQNTGHSVSSYLQLQSLNESSKDHQQYFHDVKSMKIEDQQ 306

Query: 152 DQPKKVMHHFLDEWPPKDP----ISEDKLSISNRTQLSISITNSLHDFFMT 292
            QPKKVMHHF DEWP  +      SEDK S  ++TQLSISI N  HDFF+T
Sbjct: 307 QQPKKVMHHFFDEWPKDNKDSWLDSEDKFSGLSKTQLSISIPNPSHDFFIT 357


>ref|XP_011078072.1| growth-regulating factor 1 [Sesamum indicum]
          Length = 273

 Score = 84.3 bits (207), Expect = 3e-16
 Identities = 53/107 (49%), Positives = 61/107 (57%), Gaps = 8/107 (7%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSCLENGL---QXXXXXXXXYNSDINPLNIERDDDQPKKVM 172
           SWQL PLTMG        N    +  +   Q        YN+D+     +RD DQPKKVM
Sbjct: 167 SWQLVPLTMGSTYSSGLENRQQQQLQILTDQHNHDQNHYYNTDMLLNPDQRDHDQPKKVM 226

Query: 173 HHFLDEWPPKD----PISEDKLSISNRTQLSISI-TNSLHDFFMTPK 298
           HHFLDE PPK     P  ++ LS S+RTQLSIS   NSLHDFFMT K
Sbjct: 227 HHFLDELPPKHKDSWPNPDENLSSSHRTQLSISAPPNSLHDFFMTQK 273


>ref|XP_022883235.1| growth-regulating factor 6-like, partial [Olea europaea var.
           sylvestris]
          Length = 275

 Score = 84.3 bits (207), Expect = 3e-16
 Identities = 47/97 (48%), Positives = 56/97 (57%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKVMHHF 181
           SWQ+A LT+G  +K   Y                  Y SDIN   I  +D QP K+MHHF
Sbjct: 195 SWQMAQLTLG-DVKQNPY------------------YTSDINNREIV-EDPQPNKIMHHF 234

Query: 182 LDEWPPKDPISEDKLSISNRTQLSISITNSLHDFFMT 292
           LDEWPPKD    +   +S+RTQLSIS+ N LHDFFMT
Sbjct: 235 LDEWPPKDNKDSEDKFLSHRTQLSISVPNPLHDFFMT 271


>ref|XP_009767378.1| PREDICTED: growth-regulating factor 1-like [Nicotiana sylvestris]
 ref|XP_016449440.1| PREDICTED: growth-regulating factor 1-like isoform X2 [Nicotiana
           tabacum]
          Length = 364

 Score = 80.1 bits (196), Expect = 3e-14
 Identities = 47/114 (41%), Positives = 63/114 (55%), Gaps = 17/114 (14%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSC-----------LENGLQXXXXXXXXYNSDINPLNIERD 148
           +WQL+PLTM  P+K + Y+ S            L++  +        Y +D   + +E +
Sbjct: 247 TWQLSPLTMASPMKQRTYSLSQNTGHSASSYLQLQSLNESSKDHHHQYYNDAKSIKLETE 306

Query: 149 DDQ--PKKVMHHFLDEWPPKDP----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           D Q  PKKVMHHF DEWP  +      SEDK S  ++TQLSISI N  HDFF+T
Sbjct: 307 DQQHQPKKVMHHFFDEWPKDNKDSWLDSEDKFSGLSKTQLSISIPNPSHDFFIT 360


>ref|XP_016449439.1| PREDICTED: growth-regulating factor 1-like isoform X1 [Nicotiana
           tabacum]
          Length = 385

 Score = 80.1 bits (196), Expect = 3e-14
 Identities = 47/114 (41%), Positives = 63/114 (55%), Gaps = 17/114 (14%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSC-----------LENGLQXXXXXXXXYNSDINPLNIERD 148
           +WQL+PLTM  P+K + Y+ S            L++  +        Y +D   + +E +
Sbjct: 247 TWQLSPLTMASPMKQRTYSLSQNTGHSASSYLQLQSLNESSKDHHHQYYNDAKSIKLETE 306

Query: 149 DDQ--PKKVMHHFLDEWPPKDP----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           D Q  PKKVMHHF DEWP  +      SEDK S  ++TQLSISI N  HDFF+T
Sbjct: 307 DQQHQPKKVMHHFFDEWPKDNKDSWLDSEDKFSGLSKTQLSISIPNPSHDFFIT 360


>ref|XP_004243717.1| PREDICTED: growth-regulating factor 1-like [Solanum lycopersicum]
          Length = 344

 Score = 77.0 bits (188), Expect = 3e-13
 Identities = 47/106 (44%), Positives = 60/106 (56%), Gaps = 9/106 (8%)
 Frame = +2

Query: 2   SWQLAPLTMGF-PLKDKAYNS---SCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKV 169
           +WQL+PLTM   P+K +   S   S   + LQ          +     ++E++D QPKKV
Sbjct: 235 TWQLSPLTMSSSPMKQRTTYSPSQSTAHSYLQLQSLNESSRQNYYYHNDVEKEDHQPKKV 294

Query: 170 MHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           MHHF DEWPPKD       S+DK S  +RTQLSISI N   D F+T
Sbjct: 295 MHHFFDEWPPKDNKHSWLDSDDKFSGLSRTQLSISIPNPSQDLFIT 340


>ref|XP_006342364.1| PREDICTED: growth-regulating factor 1 [Solanum tuberosum]
          Length = 340

 Score = 75.9 bits (185), Expect = 8e-13
 Identities = 46/106 (43%), Positives = 60/106 (56%), Gaps = 9/106 (8%)
 Frame = +2

Query: 2   SWQLAPLTMGF-PLKDKAYNS---SCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKV 169
           +WQL+PLTM   P+K +   S   S   + LQ          +     ++E++D QPKKV
Sbjct: 231 TWQLSPLTMSSSPMKQRTTYSPSQSTAHSYLQLQNLNESSRQNYYYHNDVEKEDHQPKKV 290

Query: 170 MHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           MHHF DEWPPKD       S+DK S  ++TQLSISI N   D F+T
Sbjct: 291 MHHFFDEWPPKDNKHSWLDSDDKFSGLSKTQLSISIPNPSQDLFIT 336


>gb|PHT41787.1| hypothetical protein CQW23_20641 [Capsicum baccatum]
          Length = 469

 Score = 76.3 bits (186), Expect = 1e-12
 Identities = 54/136 (39%), Positives = 69/136 (50%), Gaps = 23/136 (16%)
 Frame = +2

Query: 2   SWQLAPLTMGFP----LKDKAYNSSCLENGLQXXXXXXXX-YNSDIN------------- 127
           +WQL+ LTM       LK + Y+ S    G           +N+D +             
Sbjct: 259 TWQLSALTMSSSPMCHLKQRTYSPSQSTTGHSAASYLQLQSFNNDSSILQQQQRQYYHDL 318

Query: 128 PLNIERDDDQPKKVMHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           P+ IE +D QPKKVMHHF DEWPPKD       S+DK    ++TQLSISI N   DFF+T
Sbjct: 319 PMKIETEDHQPKKVMHHFFDEWPPKDNKDSWLDSDDKFLGLSKTQLSISIPNPSQDFFIT 378

Query: 293 PK*KSMKLRSGIFLGS 340
               ++ LRS  FL S
Sbjct: 379 T--NALLLRSDQFLRS 392


>ref|XP_015081067.1| PREDICTED: growth-regulating factor 5-like [Solanum pennellii]
          Length = 339

 Score = 73.9 bits (180), Expect = 4e-12
 Identities = 45/105 (42%), Positives = 59/105 (56%), Gaps = 9/105 (8%)
 Frame = +2

Query: 2   SWQLAPLTMGF-PLKDKAYNS---SCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKV 169
           +WQL+PLTM   P+K +   S   S   + LQ          +     ++E++D QPKKV
Sbjct: 230 TWQLSPLTMSSSPMKQRTTYSPSQSTAHSYLQLQSLNESSRQNYYYHNDVEKEDHQPKKV 289

Query: 170 MHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFM 289
           MHHF DEWPPKD       S+DK S  ++TQLSISI N   D F+
Sbjct: 290 MHHFFDEWPPKDNKHSWLDSDDKFSGLSKTQLSISIPNPSQDLFI 334


>ref|XP_023744379.1| growth-regulating factor 1 isoform X2 [Lactuca sativa]
 gb|PLY65699.1| hypothetical protein LSAT_5X141200 [Lactuca sativa]
          Length = 329

 Score = 73.2 bits (178), Expect = 7e-12
 Identities = 48/108 (44%), Positives = 58/108 (53%), Gaps = 11/108 (10%)
 Frame = +2

Query: 2   SWQLAPLTMGFP------LKDKAYNSSCLENGL-----QXXXXXXXXYNSDINPLNIERD 148
           SWQL PL M  P      LK +   S   +NG      +        YN  I  L I R 
Sbjct: 224 SWQLEPLAMNNPNSSLAHLKQQTSFSDYHQNGYLQYPHEQHQDQGSYYNHQIG-LKIARK 282

Query: 149 DDQPKKVMHHFLDEWPPKDPISEDKLSISNRTQLSISITNSLHDFFMT 292
           D+ P+KVMHHF DEWPP D  ++D    S+ TQLSISI +S HDFF+T
Sbjct: 283 DE-PQKVMHHFFDEWPPNDENNQD----SSTTQLSISIPSSAHDFFLT 325


>ref|XP_023744378.1| growth-regulating factor 1 isoform X1 [Lactuca sativa]
          Length = 340

 Score = 73.2 bits (178), Expect = 8e-12
 Identities = 48/108 (44%), Positives = 58/108 (53%), Gaps = 11/108 (10%)
 Frame = +2

Query: 2   SWQLAPLTMGFP------LKDKAYNSSCLENGL-----QXXXXXXXXYNSDINPLNIERD 148
           SWQL PL M  P      LK +   S   +NG      +        YN  I  L I R 
Sbjct: 224 SWQLEPLAMNNPNSSLAHLKQQTSFSDYHQNGYLQYPHEQHQDQGSYYNHQIG-LKIARK 282

Query: 149 DDQPKKVMHHFLDEWPPKDPISEDKLSISNRTQLSISITNSLHDFFMT 292
           D+ P+KVMHHF DEWPP D  ++D    S+ TQLSISI +S HDFF+T
Sbjct: 283 DE-PQKVMHHFFDEWPPNDENNQD----SSTTQLSISIPSSAHDFFLT 325


>ref|XP_016539546.1| PREDICTED: LOW QUALITY PROTEIN: growth-regulating factor 1-like
           [Capsicum annuum]
          Length = 346

 Score = 73.2 bits (178), Expect = 8e-12
 Identities = 48/120 (40%), Positives = 61/120 (50%), Gaps = 23/120 (19%)
 Frame = +2

Query: 2   SWQLAPLTMGFP----LKDKAYNSSCLENGLQXXXXXXXX-YNSDIN------------- 127
           +WQL+ LTM       LK + Y+ S    G           +N+D +             
Sbjct: 223 TWQLSALTMSSSPMCHLKQRTYSPSQSTTGHSAASYLQLQSFNNDSSILQQQQRQYYHDL 282

Query: 128 PLNIERDDDQPKKVMHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           P+ IE +D QPKKVMHHF DEWPPKD       S+DK    ++TQLSISI N   DFF+T
Sbjct: 283 PMKIETEDHQPKKVMHHFFDEWPPKDNKDSWLDSDDKFLGLSKTQLSISIPNPSQDFFIT 342


>gb|PHU10440.1| Growth-regulating factor 5, partial [Capsicum chinense]
          Length = 365

 Score = 73.2 bits (178), Expect = 9e-12
 Identities = 48/120 (40%), Positives = 61/120 (50%), Gaps = 23/120 (19%)
 Frame = +2

Query: 2   SWQLAPLTMGFP----LKDKAYNSSCLENGLQXXXXXXXX-YNSDIN------------- 127
           +WQL+ LTM       LK + Y+ S    G           +N+D +             
Sbjct: 242 TWQLSALTMSSSPMCHLKQRTYSPSQSTTGHSAASYLQLQSFNNDSSILQQQQRQYYHDL 301

Query: 128 PLNIERDDDQPKKVMHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           P+ IE +D QPKKVMHHF DEWPPKD       S+DK    ++TQLSISI N   DFF+T
Sbjct: 302 PMKIETEDHQPKKVMHHFFDEWPPKDNKDSWLDSDDKFLGLSKTQLSISIPNPSQDFFIT 361


>gb|PHT62641.1| hypothetical protein T459_33536, partial [Capsicum annuum]
          Length = 365

 Score = 73.2 bits (178), Expect = 9e-12
 Identities = 48/120 (40%), Positives = 61/120 (50%), Gaps = 23/120 (19%)
 Frame = +2

Query: 2   SWQLAPLTMGFP----LKDKAYNSSCLENGLQXXXXXXXX-YNSDIN------------- 127
           +WQL+ LTM       LK + Y+ S    G           +N+D +             
Sbjct: 242 TWQLSALTMSSSPMCHLKQRTYSPSQSTTGHSAASYLQLQSFNNDSSILQQQQRQYYHDL 301

Query: 128 PLNIERDDDQPKKVMHHFLDEWPPKDP-----ISEDKLSISNRTQLSISITNSLHDFFMT 292
           P+ IE +D QPKKVMHHF DEWPPKD       S+DK    ++TQLSISI N   DFF+T
Sbjct: 302 PMKIETEDHQPKKVMHHFFDEWPPKDNKDSWLDSDDKFLGLSKTQLSISIPNPSQDFFIT 361


>ref|XP_019225282.1| PREDICTED: growth-regulating factor 1-like [Nicotiana attenuata]
 gb|OIT32768.1| growth-regulating factor 5 [Nicotiana attenuata]
          Length = 369

 Score = 71.6 bits (174), Expect = 3e-11
 Identities = 48/118 (40%), Positives = 63/118 (53%), Gaps = 21/118 (17%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYN---------SSCLE----NGLQXXXXXXXXYNSDINPLNIE 142
           +WQL+PLTMG  +K +  +         SS L+    N           YN D+  + +E
Sbjct: 249 TWQLSPLTMGSSMKQRTNSLSQNTGHSASSYLQLQSLNESSTKDHHHQYYN-DVKGMKLE 307

Query: 143 RDD----DQPKKVMHHFLDEWPPKDP----ISEDKLSISNRTQLSISITNSLHDFFMT 292
            +D     QPKKVMHHF DEWP  +      SEDK S  ++TQLSISI N  +DFF+T
Sbjct: 308 TEDHQQQQQPKKVMHHFFDEWPKDNKDSWLDSEDKYSGLSKTQLSISIPNPSYDFFIT 365


>ref|XP_021996440.1| growth-regulating factor 1-like isoform X3 [Helianthus annuus]
          Length = 333

 Score = 69.3 bits (168), Expect = 2e-10
 Identities = 40/97 (41%), Positives = 52/97 (53%)
 Frame = +2

Query: 2   SWQLAPLTMGFPLKDKAYNSSCLENGLQXXXXXXXXYNSDINPLNIERDDDQPKKVMHHF 181
           SWQL PL M      +   S   +N           Y      L I+R+D+ P+KVMHHF
Sbjct: 236 SWQLEPLAMNNSSSKQTTFSDYHQNRYSYQQQQDPGYYDQQLALKIDRNDE-PQKVMHHF 294

Query: 182 LDEWPPKDPISEDKLSISNRTQLSISITNSLHDFFMT 292
            DEWPP D  ++D    S+ TQLSISI +S  DFF++
Sbjct: 295 FDEWPPNDDNNKDS---SSTTQLSISIPSSARDFFLS 328