BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00028437
         (1262 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011069903.1| 4-coumarate--CoA ligase 1 [Sesamum indicum]       590   0.0  
gb|AAP68990.1| 4-coumarate:coenzyme A ligase 1 [Salvia miltiorrh...   556   0.0  
ref|XP_012831310.1| PREDICTED: 4-coumarate--CoA ligase 1-like [E...   556   0.0  
ref|XP_022867626.1| 4-coumarate--CoA ligase 2-like [Olea europae...   514   e-176
gb|KZV27397.1| 4-coumarate--CoA ligase 2-like [Dorcoceras hygrom...   507   e-174
gb|ASU87411.1| 4-coumarate-CoA ligase [Camellia sinensis]             487   e-166
ref|XP_002533186.1| PREDICTED: 4-coumarate--CoA ligase 2 [Ricinu...   484   e-165
ref|XP_017982454.1| PREDICTED: 4-coumarate--CoA ligase 1 [Theobr...   481   e-164
ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Popu...   480   e-163
ref|XP_012074070.1| 4-coumarate--CoA ligase 2 [Jatropha curcas] ...   479   e-163
gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]   479   e-163
gb|APR63691.1| 4-coumarate--CoA ligase family protein 1 [Populus...   478   e-163
gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x P...   477   e-162
gb|OVA10231.1| AMP-dependent synthetase/ligase [Macleaya cordata]     475   e-162
ref|XP_021634708.1| 4-coumarate--CoA ligase 2-like [Manihot escu...   476   e-162
gb|PNT32136.1| hypothetical protein POPTR_006G169700v3 [Populus ...   476   e-162
gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]       475   e-161
gb|PKI52297.1| hypothetical protein CRG98_027223 [Punica granatum]    475   e-161
gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]        475   e-161
ref|XP_021276237.1| 4-coumarate--CoA ligase 1-like [Herrania umb...   474   e-161

>ref|XP_011069903.1| 4-coumarate--CoA ligase 1 [Sesamum indicum]
          Length = 534

 Score =  590 bits (1520), Expect = 0.0
 Identities = 309/422 (73%), Positives = 338/422 (80%), Gaps = 2/422 (0%)
 Frame = -3

Query: 1260 NLGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIIT 1081
            NLGIRK HVIMLLLHNSPE                        AEI LQA+ SKP LIIT
Sbjct: 67   NLGIRKNHVIMLLLHNSPEFAFAFLASSFVGAAATTANPLYTAAEITLQAKISKPKLIIT 126

Query: 1080 HACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            HACYV KV+ FA E GAKI+TIDPPPSP IIH SELT+SDEKLLP VEI A+D  AL FS
Sbjct: 127  HACYVGKVEHFAFEEGAKIVTIDPPPSPLIIHLSELTRSDEKLLPEVEIQAEDMPALPFS 186

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTG PKGVMLSHKN+VTCV+Q+VDGE P FH+  +DLTVCVLPLFH++SLI VLL ++
Sbjct: 187  SGTTGFPKGVMLSHKNLVTCVSQQVDGENPAFHLDVKDLTVCVLPLFHVYSLITVLLCSL 246

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXA-VFDLSSVRRVIC 547
            R GAAVLI+PK+EIN LME IQKYRVTIAPFV              A  FDLSSVRRV C
Sbjct: 247  RAGAAVLILPKYEINALMELIQKYRVTIAPFVPPILLAIAKIPAAAAGKFDLSSVRRVTC 306

Query: 546  GAAPMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            GAAPMDK+LEG+ K KLPNA IGQGYGMTEAGV++MCLGFA       PGSCGTVIRN R
Sbjct: 307  GAAPMDKQLEGAVKCKLPNAMIGQGYGMTEAGVLTMCLGFAKRPFRFKPGSCGTVIRNAR 366

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MK++DPSTG SL RNQTGEI +RGDAVMKGYYN+ EAT+RT+D +GWLHTGDIGYVDDDD
Sbjct: 367  MKVIDPSTGVSLPRNQTGEICIRGDAVMKGYYNEPEATKRTVDKEGWLHTGDIGYVDDDD 426

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            EVFIVDRLKELIKYKGFH+APAELEALLIAHPSISDAAVVPM DE AGEVPVAFVVPANG
Sbjct: 427  EVFIVDRLKELIKYKGFHIAPAELEALLIAHPSISDAAVVPMRDEGAGEVPVAFVVPANG 486

Query: 6    SN 1
            S+
Sbjct: 487  SS 488


>gb|AAP68990.1| 4-coumarate:coenzyme A ligase 1 [Salvia miltiorrhiza]
          Length = 535

 Score =  556 bits (1434), Expect = 0.0
 Identities = 287/420 (68%), Positives = 330/420 (78%), Gaps = 1/420 (0%)
 Frame = -3

Query: 1260 NLGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIIT 1081
            NLGIRK  V+ LLLHNSPE                        +EI LQA+ S+P LI+T
Sbjct: 70   NLGIRKSDVVTLLLHNSPEFAFAFLGASFIGAISTTANPLYTASEIALQARISRPKLIVT 129

Query: 1080 HACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            HAC+VEKVK +A E GAKI TIDPPPSP+IIHF+EL +SDEKLL  +EIHADDT AL FS
Sbjct: 130  HACHVEKVKHYAAEAGAKIATIDPPPSPEIIHFAELRRSDEKLLTPIEIHADDTVALPFS 189

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLLN-M 724
            SGTTGLPKGVMLSHKN+V CV+Q+VDGE P  H+ R D  +CVLPLFH++S+I V+L  +
Sbjct: 190  SGTTGLPKGVMLSHKNLVACVSQQVDGENPAVHIDREDRMLCVLPLFHVYSMISVMLCCL 249

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVGAAV+I+PKFEI+ LME I+KYRVTIAPFV                FD SSVRRV+CG
Sbjct: 250  RVGAAVVIMPKFEISELMELIEKYRVTIAPFVPPILLAIAKSPAAAK-FDFSSVRRVVCG 308

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            AAPMD++LE + KAKLPNA IGQGYGMTEAGV+SM LGFA        GSCGTVIRN RM
Sbjct: 309  AAPMDRELELALKAKLPNAVIGQGYGMTEAGVLSMSLGFAKRPLKFKAGSCGTVIRNARM 368

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIVDPS+ ASL RN+TGEI ++GDAVMKGYYND EATRRTID +GWLHTGD+G+VDDD+E
Sbjct: 369  KIVDPSSAASLPRNETGEICIKGDAVMKGYYNDPEATRRTIDEEGWLHTGDLGFVDDDEE 428

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            V+IVDRLKELIKYKGFH+APAELEALL+AHPSIS+AAVVPM DEAAGEVPVAFVV AN +
Sbjct: 429  VYIVDRLKELIKYKGFHIAPAELEALLVAHPSISEAAVVPMADEAAGEVPVAFVVRANAA 488


>ref|XP_012831310.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Erythranthe guttata]
 gb|EYU42437.1| hypothetical protein MIMGU_mgv1a004106mg [Erythranthe guttata]
          Length = 543

 Score =  556 bits (1433), Expect = 0.0
 Identities = 295/427 (69%), Positives = 328/427 (76%), Gaps = 8/427 (1%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGIRK  V+MLLLHNSP+                        AEI  QA+ SKP LI+TH
Sbjct: 71   LGIRKNDVVMLLLHNSPDFAFAFLAAFFAGAVATTANPLYTAAEITAQARVSKPKLIVTH 130

Query: 1077 ACYVEKVKRFADEL-----GAKII--TIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDT 919
            ACYVEKVKRFA E      GA +I  TIDPPPSP+I+ F++L  SDE+LLP VEI ADDT
Sbjct: 131  ACYVEKVKRFAVEEEERGGGAVVIIATIDPPPSPEIVRFADLKNSDERLLPEVEIDADDT 190

Query: 918  AALLFSSGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIM 739
            AAL FSSGTTGLPKGVMLSHKNIVTC++QKVDGE P +H+  RDL +C+LPLFH++ L+ 
Sbjct: 191  AALPFSSGTTGLPKGVMLSHKNIVTCISQKVDGENPAYHIDSRDLMLCLLPLFHVYCLVS 250

Query: 738  VLL-NMRVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSV 562
            VLL ++RVGAA+LIVPKFEIN LME IQK+RVTIAP V                FDLSSV
Sbjct: 251  VLLTSLRVGAAILIVPKFEINQLMELIQKHRVTIAPLVPPILLAISKSPAAAVRFDLSSV 310

Query: 561  RRVICGAAPMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTV 382
            RRV CGAAPMD +LE S KAKLPNATIGQGYGMTEAGV+SMCLGFA       PGSCG V
Sbjct: 311  RRVTCGAAPMDSQLECSVKAKLPNATIGQGYGMTEAGVLSMCLGFAKKPFKFKPGSCGNV 370

Query: 381  IRNYRMKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGY 202
            IRN  MKIV+P TGASL RNQTGEI +RGD+VMKGYYND EAT+RTID DGWLHTGDIGY
Sbjct: 371  IRNSEMKIVNPLTGASLPRNQTGEICIRGDSVMKGYYNDPEATKRTIDKDGWLHTGDIGY 430

Query: 201  VDDDDEVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFV 22
            VD DDEVFI+DRLKELIKYKG  +APAELEALLIAHPS+S+AAVVPM DE AGEVPVAFV
Sbjct: 431  VDSDDEVFILDRLKELIKYKGLQIAPAELEALLIAHPSVSEAAVVPMKDEVAGEVPVAFV 490

Query: 21   VPANGSN 1
            V  NGSN
Sbjct: 491  VRVNGSN 497


>ref|XP_022867626.1| 4-coumarate--CoA ligase 2-like [Olea europaea var. sylvestris]
 ref|XP_022867627.1| 4-coumarate--CoA ligase 2-like [Olea europaea var. sylvestris]
          Length = 558

 Score =  514 bits (1323), Expect = e-176
 Identities = 268/419 (63%), Positives = 309/419 (73%), Gaps = 1/419 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VIMLLLHNSPE                        AEI+ QA  SKP LIITH
Sbjct: 95   LGIQQGQVIMLLLHNSPEFVFAFLGASYIGAITTTANPLYTPAEIEKQATISKPYLIITH 154

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            A YV+KVK FA + G KI  IDPPP     HFSELT++DE  +P+V +H DD   L FSS
Sbjct: 155  AIYVQKVKNFAQQNGVKIFCIDPPPE-SCFHFSELTQTDENSIPSVNVHPDDMVVLPFSS 213

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTG PKGVML+HKN+VTCV+Q+VDGE P   V   DLT+CVLPLFH++SLI V+L  +R
Sbjct: 214  GTTGFPKGVMLTHKNLVTCVSQQVDGENPTLCVQPEDLTICVLPLFHVYSLITVMLCTLR 273

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VG+A+LI+ KFEIN LME +QKY+VTIAPFV                FDLSSVRRV+CGA
Sbjct: 274  VGSAILIMQKFEINALMENVQKYKVTIAPFVPPILLAIAKSPVADR-FDLSSVRRVVCGA 332

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTVIRNYRMK 361
            APMDK LE + + KLPNA IGQGYGMTE GV+SMCLGFA        GSCGTVIRN +MK
Sbjct: 333  APMDKNLEDAVRRKLPNAKIGQGYGMTEGGVVSMCLGFAKEAFQFKSGSCGTVIRNAQMK 392

Query: 360  IVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDEV 181
            I DP TG SL  N+ GEI +RGD +MKGY ND EAT+RTID +GW+HTGDIGYVD+++E+
Sbjct: 393  INDPQTGLSLPYNRAGEIWIRGDQIMKGYLNDPEATKRTIDREGWMHTGDIGYVDENEEL 452

Query: 180  FIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            FIVDRLKELIKYKGFH+APAELEALLI HPSISDAAVVPM D+AAGEVPVAFVV  NGS
Sbjct: 453  FIVDRLKELIKYKGFHIAPAELEALLIVHPSISDAAVVPMNDDAAGEVPVAFVVRENGS 511


>gb|KZV27397.1| 4-coumarate--CoA ligase 2-like [Dorcoceras hygrometricum]
          Length = 536

 Score =  507 bits (1305), Expect = e-174
 Identities = 265/419 (63%), Positives = 312/419 (74%), Gaps = 1/419 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            +GIR+ HVIMLLLHNSPE                        AEI  QA+ SKP LI+TH
Sbjct: 72   IGIRQGHVIMLLLHNSPEFVFAFLAASYIGATTTTANPLYSAAEIDFQAKISKPKLIVTH 131

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            AC++ KV+ +A   GA I+ +D PP P + H SEL KSDE L+P V+IH+DDTA L FSS
Sbjct: 132  ACHLHKVEDYALNNGAVIVCVDSPPLPHL-HLSELKKSDETLMPEVKIHSDDTAVLPFSS 190

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPK VMLSHKNIVTCV+ +VDGE P  HV   DLT+CVLPLFH++S+I ++L ++R
Sbjct: 191  GTTGLPKAVMLSHKNIVTCVSVQVDGENPTLHVHAGDLTLCVLPLFHVYSMITIMLCSLR 250

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VG+ +LI+ K+EIN LM  IQKY+V+IAPFV              A FDLSSVRRV+CGA
Sbjct: 251  VGSGILIMQKYEINELMGLIQKYKVSIAPFVPPILLAMAKSPEAGAKFDLSSVRRVMCGA 310

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTVIRNYRMK 361
            A MD+KL+ S + KLPNAT+GQGYGMTEAGV+SMCLGFA        GSCG+VIRN RMK
Sbjct: 311  AAMDRKLQESVREKLPNATLGQGYGMTEAGVLSMCLGFAKKPFKFKYGSCGSVIRNARMK 370

Query: 360  IVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDEV 181
            IVDP T ASL RNQ GEI + GD VM GYYND EAT++TID +GWLHTGDIGY+D+DDE+
Sbjct: 371  IVDPLTRASLGRNQPGEICITGDQVMLGYYNDPEATKKTIDEEGWLHTGDIGYIDEDDEL 430

Query: 180  FIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            FIVDRLKELIK KGFHVAPAE+EALL+AHP+ISDAAVV   DE  GEVPVAFVV A GS
Sbjct: 431  FIVDRLKELIKCKGFHVAPAEIEALLLAHPAISDAAVVARKDEVTGEVPVAFVVRAKGS 489


>gb|ASU87411.1| 4-coumarate-CoA ligase [Camellia sinensis]
          Length = 543

 Score =  487 bits (1253), Expect = e-166
 Identities = 260/421 (61%), Positives = 303/421 (71%), Gaps = 2/421 (0%)
 Frame = -3

Query: 1260 NLGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIIT 1081
            NLGIR+  VIMLLLHNSPE                        AEI+ QA+ SK  LIIT
Sbjct: 71   NLGIRQGDVIMLLLHNSPEFVFSFLGASYSGITITTANPFYTPAEIEKQAKASKAKLIIT 130

Query: 1080 HACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
             +CYVEKV  F  E   KI++ D  P    +HFSEL+++DE   PAV+I+ DD  AL +S
Sbjct: 131  QSCYVEKVTEFGKENDIKIVSTDSTPGNSCLHFSELSQADETNAPAVKINPDDVVALPYS 190

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +    D+ +CVLPLFHI+SL  VLL  +
Sbjct: 191  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSEDVIMCVLPLFHIYSLNSVLLCAL 250

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVGAA+LI+ KFEI LLME + KY+VTIAPF                 +DLSS+R V+ G
Sbjct: 251  RVGAAILIMQKFEIKLLMELVHKYKVTIAPFAPPIVLAIANSPVFDD-YDLSSIRTVMSG 309

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
             APM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        GSCGTV+RN  
Sbjct: 310  GAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGSCGTVVRNAE 369

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIVDP TGASL +NQ GEI +RGD +MKGY ND EAT RTID +GWLHTGDIGY+DD+D
Sbjct: 370  MKIVDPDTGASLPQNQAGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGYIDDND 429

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LIAHP ISDAAVVPM DEAAGEVPVAFV+ ANG
Sbjct: 430  ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDAAVVPMKDEAAGEVPVAFVIRANG 489

Query: 6    S 4
            S
Sbjct: 490  S 490


>ref|XP_002533186.1| PREDICTED: 4-coumarate--CoA ligase 2 [Ricinus communis]
 gb|EEF29200.1| AMP dependent CoA ligase, putative [Ricinus communis]
          Length = 557

 Score =  484 bits (1246), Expect = e-165
 Identities = 260/425 (61%), Positives = 305/425 (71%), Gaps = 7/425 (1%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VIM+LL NSPE                        AEIK QA  S   LIIT 
Sbjct: 81   LGIKQNEVIMILLQNSPEFVFAFLGASVLGAISTTANPFYTPAEIKKQASASNAKLIITQ 140

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSP-----KIIHFSELTKSDEKLLPAVEIHADDTAA 913
            A YVEKVK FA+E G KI+TID PP         +HFSELTK+DE  +PAV+I+ DD  A
Sbjct: 141  AAYVEKVKDFANENGIKIMTIDSPPPALDDCRDCLHFSELTKADENDIPAVKINPDDVVA 200

Query: 912  LLFSSGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVL 733
            L +SSGTTGLPKGVML+HK++VT VAQ+VDGE PN +   +D+ +C+LPLFHI+SL  VL
Sbjct: 201  LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSLNSVL 260

Query: 732  L-NMRVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRR 556
            L  +RVGAA+LI+ KFEI  LME +QKY+VTIAPFV                +DLSS+R 
Sbjct: 261  LCGLRVGAAILIMQKFEITALMELVQKYKVTIAPFVPPIVLSIAKSPAVDK-YDLSSIRT 319

Query: 555  VICGAAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVI 379
            V+ GAAPM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+
Sbjct: 320  VMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVV 379

Query: 378  RNYRMKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYV 199
            RN  MKIVDP TG SL RNQ GEI +RG  +MKGY ND EAT RTID +GWLHTGD+GY+
Sbjct: 380  RNAEMKIVDPDTGKSLQRNQAGEICIRGSQIMKGYLNDPEATERTIDKEGWLHTGDVGYI 439

Query: 198  DDDDEVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVV 19
            D DDE+FIVDRLKELIKY GF VAPAELEA+LIAHPSISDAAVVPM DEAAGE+P AFVV
Sbjct: 440  DGDDELFIVDRLKELIKYNGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGEIPAAFVV 499

Query: 18   PANGS 4
             +NGS
Sbjct: 500  RSNGS 504


>ref|XP_017982454.1| PREDICTED: 4-coumarate--CoA ligase 1 [Theobroma cacao]
 gb|EOY31810.1| 4-coumarate:CoA ligase 2 [Theobroma cacao]
          Length = 542

 Score =  481 bits (1237), Expect = e-164
 Identities = 262/421 (62%), Positives = 307/421 (72%), Gaps = 2/421 (0%)
 Frame = -3

Query: 1260 NLGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIIT 1081
            NLGI++  VIMLLL NSPE                        AEI  QA  SK  L+IT
Sbjct: 71   NLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIVKQATASKTRLLIT 130

Query: 1080 HACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
             A Y EKVK FA E   KIITID PP    +HFSELT++DE  +PAV+I+ DD  AL +S
Sbjct: 131  QAVYAEKVKNFAKENDIKIITIDTPPEG-CMHFSELTQADESEIPAVKINPDDVVALPYS 189

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK++VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL ++
Sbjct: 190  SGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSL 249

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVGAA+LI+ KFEI  LME +Q Y+VTIAPFV                +DLSS+R V+ G
Sbjct: 250  RVGAAILIMQKFEIVTLMELVQNYKVTIAPFVPPIVLAIAKTPDVDK-YDLSSIRTVMSG 308

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +A+LPNA +GQGYGMTEAG V++M L FA        GSCGTV+RN  
Sbjct: 309  AAPMGKELEDAVRARLPNAKLGQGYGMTEAGPVLAMNLAFAKEACETKSGSCGTVVRNAE 368

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIV+P TGASL RNQ+GEI +RG  +MKGY ND EAT+RTID DGWLHTGDIGY+DDDD
Sbjct: 369  MKIVNPDTGASLPRNQSGEICIRGSQIMKGYLNDPEATQRTIDKDGWLHTGDIGYIDDDD 428

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            EVFIVDRLKELIKYKGF VAPAELEA+LI HP+ISDAAVVPM DEAAGEVPVAFVV +NG
Sbjct: 429  EVFIVDRLKELIKYKGFQVAPAELEAMLIGHPNISDAAVVPMKDEAAGEVPVAFVVRSNG 488

Query: 6    S 4
            S
Sbjct: 489  S 489


>ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Populus trichocarpa]
 gb|PNT32135.1| hypothetical protein POPTR_006G169600v3 [Populus trichocarpa]
          Length = 543

 Score =  480 bits (1236), Expect = e-163
 Identities = 254/419 (60%), Positives = 299/419 (71%), Gaps = 1/419 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VI+LLL NSPE                        AE+  QA  SK  LIIT 
Sbjct: 73   LGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQATASKAKLIITQ 132

Query: 1077 ACYVEKVKRFADE-LGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            A Y EKV+ F  E +  KI+T+D PP    +HFSELT SDE  +PAVEI+ DD  AL +S
Sbjct: 133  AVYAEKVQEFVKENVHVKIVTVDSPPE-NYLHFSELTNSDEDDIPAVEINPDDVVALPYS 191

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLLNMR 721
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL + L  +R
Sbjct: 192  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNIYFHEKDVILCVLPLFHIYSLNVFLSGLR 251

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VG+A+L++ KFEI  LME +QKY+VTIAPFV                +DLSS+R V+ GA
Sbjct: 252  VGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPVVLAVAKCPVVDK-YDLSSIRTVMSGA 310

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAGVISMCLGFAXXXXXXXPGSCGTVIRNYRMK 361
            APM K+LE + +AKLPNA +GQGYGMTEAG +SMCL FA        G+CGTV+RN  MK
Sbjct: 311  APMGKELEDTVRAKLPNAKLGQGYGMTEAGPLSMCLAFAKEPFEIKSGACGTVVRNAEMK 370

Query: 360  IVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDEV 181
            IVDP TG SL RNQ GEI +RG  +MKGY ND EAT RT+DNDGWLHTGDIGY+D DDE+
Sbjct: 371  IVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPEATERTVDNDGWLHTGDIGYIDGDDEL 430

Query: 180  FIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            FIVDRLKELIKYKGF VAPAELEA+LIAHP ISD AVVPM DEAAGEVP+AFVV ANGS
Sbjct: 431  FIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGS 489


>ref|XP_012074070.1| 4-coumarate--CoA ligase 2 [Jatropha curcas]
 gb|KDP36410.1| hypothetical protein JCGZ_08679 [Jatropha curcas]
          Length = 543

 Score =  479 bits (1234), Expect = e-163
 Identities = 256/420 (60%), Positives = 302/420 (71%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VIMLLL NSPE                        AEIK Q   SK  L+IT 
Sbjct: 73   LGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIKKQLTASKAQLVITQ 132

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            A Y EKVK+FA +   K++TID PP    +HFSELT  DE  +P V+I++DD  AL +SS
Sbjct: 133  AVYAEKVKQFAKDSDIKVMTIDSPPED-CLHFSELTSCDENDIPTVKINSDDVVALPYSS 191

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTG PKGVML+HK +VT VAQ+VDGE PN +   +D+ +C+LPLFHI+SL  VLL  +R
Sbjct: 192  GTTGFPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSLNSVLLCGLR 251

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VGAA+LI+ KFEIN LME + KY+VTIAPFV                +DLSS+R V+ GA
Sbjct: 252  VGAAILIMQKFEINSLMELVTKYKVTIAPFVPPIVLAIAKSPVVDK-YDLSSIRTVMSGA 310

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  M
Sbjct: 311  APMGKELEDAVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEM 370

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIVDP TG SL RNQ GEI +RG  +MKGY ND EAT RTID DGWLHTGD+GY+DDDDE
Sbjct: 371  KIVDPDTGNSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTGDVGYIDDDDE 430

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+LIAHP+ISDAAVVPM DEAAGE+PVAFVV +NGS
Sbjct: 431  LFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVAFVVRSNGS 490


>gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]
          Length = 545

 Score =  479 bits (1234), Expect = e-163
 Identities = 260/421 (61%), Positives = 302/421 (71%), Gaps = 3/421 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VI+LLL NSPE                        AE+  QA  SK  LIIT 
Sbjct: 73   LGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQATASKAKLIITQ 132

Query: 1077 ACYVEKVKRFADELG-AKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            A Y EKV++F  E    KI+T+D PP    +HFSELT SDE  +PAVEI+ DD  AL +S
Sbjct: 133  AVYAEKVQQFVKENDHVKIVTVDSPPE-NYLHFSELTNSDEDDIPAVEINPDDVVALPYS 191

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL  +
Sbjct: 192  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGL 251

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVG+A+L++ KFEI  LME +QKY+VTIAPFV                +DLSS+R V+ G
Sbjct: 252  RVGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPVVLAVAKCPVVDK-YDLSSIRTVMSG 310

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  
Sbjct: 311  AAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAREPFEIKSGACGTVVRNAE 370

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIVDP TG SL RNQ GEI +RG  +MKGY ND EAT RTIDNDGWLHTGDIGY+DDDD
Sbjct: 371  MKIVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDNDGWLHTGDIGYIDDDD 430

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LIAHP ISD AVVPM DEAAGEVPVAFVV ANG
Sbjct: 431  ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPVAFVVRANG 490

Query: 6    S 4
            S
Sbjct: 491  S 491


>gb|APR63691.1| 4-coumarate--CoA ligase family protein 1 [Populus tomentosa]
          Length = 545

 Score =  478 bits (1231), Expect = e-163
 Identities = 258/421 (61%), Positives = 302/421 (71%), Gaps = 3/421 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VI+LLL NSPE                        +E+  QA  SK  LIIT 
Sbjct: 73   LGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPSEVAKQATASKAKLIITQ 132

Query: 1077 ACYVEKVKRFADELG-AKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            A Y EKV++F  E    K++T+D PP    +HFSELT SDE  +PAVEI+ DD  AL +S
Sbjct: 133  AVYAEKVQQFVKENDHVKLVTVDSPPE-NYLHFSELTNSDEDDVPAVEINPDDVVALPYS 191

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL  +
Sbjct: 192  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGL 251

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVG+A+L++ KFEI  LME +QKY+VTIAPFV                +DLSS+R V+ G
Sbjct: 252  RVGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPIVLAVAKCPVVDK-YDLSSIRTVMSG 310

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  
Sbjct: 311  AAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAE 370

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIVDP TG SL RNQ GEI +RG  +MKGY ND EAT RTIDNDGWLHTGDIGY+DDDD
Sbjct: 371  MKIVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDNDGWLHTGDIGYIDDDD 430

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LIAHP ISD AVVPM DEAAGEVPVAFVV ANG
Sbjct: 431  ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPVAFVVRANG 490

Query: 6    S 4
            S
Sbjct: 491  S 491


>gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x Populus deltoides]
          Length = 557

 Score =  477 bits (1228), Expect = e-162
 Identities = 257/421 (61%), Positives = 302/421 (71%), Gaps = 3/421 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VI+LLL NSPE                        AE+  QA  SK  LIIT 
Sbjct: 73   LGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQATASKAKLIITQ 132

Query: 1077 ACYVEKVKRFADE-LGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            A Y EKV+ F  E +  KI+T+D PP    +HFSELT SDE  +PAVEI+ DD  AL +S
Sbjct: 133  AVYAEKVQEFVKENVHVKIVTVDSPPE-NYLHFSELTNSDEDDIPAVEINPDDVVALPYS 191

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL  +
Sbjct: 192  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGL 251

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVG+A+L++ KFEI  LME +QKY+VTIAPFV                +DLSS+R V+ G
Sbjct: 252  RVGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPVVLAVAKCPVVDK-YDLSSIRTVMSG 310

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  
Sbjct: 311  AAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAE 370

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIVDP TG SL RNQ+GEI +RG  +MKGY ND EAT RT+DNDGWLHTGDIGY+D DD
Sbjct: 371  MKIVDPDTGRSLPRNQSGEICIRGSQIMKGYLNDPEATERTVDNDGWLHTGDIGYIDGDD 430

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LIAHP ISD AVVPM DEAAGEVP+AFVV ANG
Sbjct: 431  ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANG 490

Query: 6    S 4
            S
Sbjct: 491  S 491


>gb|OVA10231.1| AMP-dependent synthetase/ligase [Macleaya cordata]
          Length = 509

 Score =  475 bits (1223), Expect = e-162
 Identities = 256/420 (60%), Positives = 303/420 (72%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            +GI++  VIM+LL NSPE                        AEI  QA+ S   LIIT 
Sbjct: 38   IGIQQGEVIMILLPNSPEFVFAFLGASYRGAITTTANPFYTSAEISKQAKASNARLIITQ 97

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            ACYVEK+     E G KI+ ID PP    +HFSELT+SDE  LP V+I++DD  AL +SS
Sbjct: 98   ACYVEKLNELTHENGVKIMCIDSPPEG-CLHFSELTQSDETELPHVDINSDDVVALPYSS 156

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPKGVML+HK ++T VAQ+VDGE PN +    D+ +CVLPLFHI+SL  VLL  +R
Sbjct: 157  GTTGLPKGVMLTHKGLITSVAQQVDGENPNLYFNSDDVLLCVLPLFHIYSLNSVLLCGLR 216

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VG+A+LI+ KFEI  LME +QKY+VTIAPFV                +DLSS+R ++ GA
Sbjct: 217  VGSAILIMQKFEIISLMELVQKYKVTIAPFVPPIVLAIAKSPVVDK-YDLSSIRTIMSGA 275

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        GSCGTV+RN  M
Sbjct: 276  APMGKELEDTLRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFDIKSGSCGTVVRNAEM 335

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIVDP TGASL RNQ+GEI +RG  +MKGY ND EAT+ TID DGWLHTGDIGY+DDDDE
Sbjct: 336  KIVDPETGASLPRNQSGEICIRGHQIMKGYLNDPEATKNTIDKDGWLHTGDIGYIDDDDE 395

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+LIA+P+ISDAAVV M DEAAGEVPVAFVV +NGS
Sbjct: 396  IFIVDRLKELIKYKGFQVAPAELEAMLIANPNISDAAVVSMKDEAAGEVPVAFVVRSNGS 455


>ref|XP_021634708.1| 4-coumarate--CoA ligase 2-like [Manihot esculenta]
          Length = 542

 Score =  476 bits (1226), Expect = e-162
 Identities = 256/420 (60%), Positives = 304/420 (72%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++ +VIMLLL NSPE                         EI  QA  SK  LI+T 
Sbjct: 72   LGIQQGYVIMLLLQNSPEFVFVFLGASYRGAISTTANPFYTPTEIAKQATASKAKLIVTQ 131

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            A Y EKVK+FA E   KIITID PP    +HFSELT++DE  +P V+I+ DD  AL +SS
Sbjct: 132  AVYAEKVKQFAKENDIKIITIDSPPDG-CLHFSELTEADENDIPGVKINPDDVVALPYSS 190

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPKGVML+HK ++T VAQ+VDG+ PN +  ++D+ +CVLPLFHI+SL  VLL  +R
Sbjct: 191  GTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHKKDVILCVLPLFHIYSLNSVLLCGLR 250

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VG+A+LI+ KFEI  LME +QKY+V+IAPFV                +DLSS+R V+ GA
Sbjct: 251  VGSAILIMQKFEIISLMELVQKYKVSIAPFVPPIVLAIAKSPVVDQ-YDLSSIRTVMSGA 309

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  M
Sbjct: 310  APMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKDPFEIKSGACGTVVRNAEM 369

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIVDP TG SL RNQ GEI +RG  +MKGY ND EAT  TID +GWLHTGDIGY+DDDDE
Sbjct: 370  KIVDPETGNSLPRNQAGEICIRGSQIMKGYLNDPEATISTIDKEGWLHTGDIGYIDDDDE 429

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+LI+HPSISDAAVVPM DEAAGEVPVAFVV +NGS
Sbjct: 430  LFIVDRLKELIKYKGFQVAPAELEAMLISHPSISDAAVVPMKDEAAGEVPVAFVVRSNGS 489


>gb|PNT32136.1| hypothetical protein POPTR_006G169700v3 [Populus trichocarpa]
          Length = 545

 Score =  476 bits (1225), Expect = e-162
 Identities = 257/421 (61%), Positives = 301/421 (71%), Gaps = 3/421 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI++  VI+LLL NSPE                        AE+  QA  SK  LIIT 
Sbjct: 73   LGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQATASKAKLIITQ 132

Query: 1077 ACYVEKVKRFADELG-AKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
            A Y EKV++F  E    KI+T+D PP    +HFSELT SDE  +PAVEI+ DD  AL +S
Sbjct: 133  AVYAEKVQQFVKENDHVKIVTVDSPPE-NYLHFSELTNSDEDDIPAVEINPDDVVALPYS 191

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK +VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL  +
Sbjct: 192  SGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGL 251

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVG+A+L++ KFEI  LME +QKY+VTIAPFV                +DLSS+R V+ G
Sbjct: 252  RVGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPVVLAVAKCPVVDK-YDLSSIRTVMSG 310

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +AKLPNA +GQGYGMTEAG V+SMCL FA        G+CGTV+RN  
Sbjct: 311  AAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAE 370

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIVDP TG SL RNQ GEI +RG  +MKGY ND EAT RT+DNDGWLHTGDIGY+D DD
Sbjct: 371  MKIVDPDTGRSLPRNQAGEICIRGSQIMKGYLNDPEATERTVDNDGWLHTGDIGYIDGDD 430

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LIAHP ISD AVVPM DEAAGEVP+AFVV ANG
Sbjct: 431  ELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANG 490

Query: 6    S 4
            S
Sbjct: 491  S 491


>gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]
          Length = 543

 Score =  475 bits (1222), Expect = e-161
 Identities = 253/420 (60%), Positives = 303/420 (72%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            +GI++  VIMLLL NSPE                        AEI+ QA+ SK  LIIT 
Sbjct: 72   IGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQARASKAKLIITQ 131

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            +CYVEKV  FA E   K++  D PP    +HFSEL+ +DEK++PAV+I+ +D  AL +SS
Sbjct: 132  SCYVEKVMDFAKENNVKVMCTDAPPEG-CLHFSELSSADEKVIPAVKINPNDAVALPYSS 190

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPKGVML+HK +VT VAQ+VDGE PN +  + D+ +CVLPLFHI+SL  VLL  +R
Sbjct: 191  GTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLCGLR 250

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VGAA+LI+ KFEIN LME +QKY+VTIAPFV                +DLSS+R V+ GA
Sbjct: 251  VGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDK-YDLSSIRMVMSGA 309

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE + +AKLP A +GQGYGMTEAG ++SMCL FA        G+CGTV+RN  M
Sbjct: 310  APMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKSGACGTVVRNAEM 369

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIVDP T  SL RNQ GEI +RGD +MKGY ND EAT  TID +GWLHTGDIGY+DDDDE
Sbjct: 370  KIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLHTGDIGYIDDDDE 429

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+L++HP ISDAAVV M DEAAGEVPVAFVV A+GS
Sbjct: 430  IFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGEVPVAFVVRASGS 489


>gb|PKI52297.1| hypothetical protein CRG98_027223 [Punica granatum]
          Length = 544

 Score =  475 bits (1222), Expect = e-161
 Identities = 255/420 (60%), Positives = 303/420 (72%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI +  VIMLLLHNSPE                         EI  QA  SK  LIIT 
Sbjct: 74   LGIEQGQVIMLLLHNSPEFVHAFLGASFCGAMSTTANPFCTPPEIAKQAAASKARLIITQ 133

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            A +  KVK FA+E G K++ ID  P    +HFSELT++DE  +P+V+I  +D  AL +SS
Sbjct: 134  AEFAPKVKDFAEENGVKVMCIDSAPEG-CLHFSELTQADEAEMPSVKIDPNDVVALPYSS 192

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPKGVML+HK +VT VAQ+VDGE PN +  + D+ +CVLPLFHI+SL  ++L  +R
Sbjct: 193  GTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRKEDVILCVLPLFHIYSLNSIMLCGLR 252

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VGAA+LI+ KFEI  LME +Q+Y+VTIAPFV                +DLSS+R V+ GA
Sbjct: 253  VGAAILIMQKFEIVKLMELVQRYKVTIAPFVPPIVLAMSKYADMDK-YDLSSIRTVMSGA 311

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE S +AKLPNA +GQGYGMTEAG V++MCL FA        G+CGTV+RN  M
Sbjct: 312  APMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEM 371

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIV+P TGASL RNQ GEI +RG+ +MKGY ND EAT RTID DGWLHTGDIGY+DDDDE
Sbjct: 372  KIVNPDTGASLPRNQPGEICIRGNQIMKGYLNDPEATERTIDKDGWLHTGDIGYIDDDDE 431

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+LIAHPSISDAAVVPM DEAAGEVPVAFV+ +NGS
Sbjct: 432  LFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGEVPVAFVIRSNGS 491


>gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]
          Length = 544

 Score =  475 bits (1222), Expect = e-161
 Identities = 255/420 (60%), Positives = 303/420 (72%), Gaps = 2/420 (0%)
 Frame = -3

Query: 1257 LGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIITH 1078
            LGI +  VIMLLLHNSPE                         EI  QA  SK  LIIT 
Sbjct: 74   LGIEQGQVIMLLLHNSPEFVHAFLGASFCGAMSTTANPFCTPPEIAKQAAASKARLIITQ 133

Query: 1077 ACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFSS 898
            A +  KVK FA+E G K++ ID  P    +HFSELT++DE  +P+V+I  +D  AL +SS
Sbjct: 134  AEFAPKVKDFAEENGVKVMCIDSAPEG-CLHFSELTQADEAEMPSVKIDPNDVVALPYSS 192

Query: 897  GTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NMR 721
            GTTGLPKGVML+HK +VT VAQ+VDGE PN +  + D+ +CVLPLFHI+SL  ++L  +R
Sbjct: 193  GTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRKEDVILCVLPLFHIYSLNSIMLCGLR 252

Query: 720  VGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICGA 541
            VGAA+LI+ KFEI  LME +Q+Y+VTIAPFV                +DLSS+R V+ GA
Sbjct: 253  VGAAILIMQKFEIVKLMELVQRYKVTIAPFVPPIVLAMSKYADMDK-YDLSSIRTVMSGA 311

Query: 540  APMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYRM 364
            APM K+LE S +AKLPNA +GQGYGMTEAG V++MCL FA        G+CGTV+RN  M
Sbjct: 312  APMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEM 371

Query: 363  KIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDDE 184
            KIV+P TGASL RNQ GEI +RG+ +MKGY ND EAT RTID DGWLHTGDIGY+DDDDE
Sbjct: 372  KIVNPDTGASLPRNQPGEICIRGNQIMKGYLNDPEATERTIDKDGWLHTGDIGYIDDDDE 431

Query: 183  VFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANGS 4
            +FIVDRLKELIKYKGF VAPAELEA+LIAHPSISDAAVVPM DEAAGEVPVAFV+ +NGS
Sbjct: 432  LFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGEVPVAFVIRSNGS 491


>ref|XP_021276237.1| 4-coumarate--CoA ligase 1-like [Herrania umbratica]
          Length = 542

 Score =  474 bits (1221), Expect = e-161
 Identities = 258/421 (61%), Positives = 305/421 (72%), Gaps = 2/421 (0%)
 Frame = -3

Query: 1260 NLGIRKRHVIMLLLHNSPEXXXXXXXXXXXXXXXXXXXXXXXXAEIKLQAQKSKPTLIIT 1081
            NLGI++  VIMLLL NSPE                        AEI  QA  SK  L+IT
Sbjct: 71   NLGIQQGDVIMLLLQNSPEFVFAFLGASFLGAISTTANPFYTPAEIVKQATASKTRLLIT 130

Query: 1080 HACYVEKVKRFADELGAKIITIDPPPSPKIIHFSELTKSDEKLLPAVEIHADDTAALLFS 901
             A Y EKVK FA E   KIITID PP    +HFSELT++DE  +P V+I+ DD  AL +S
Sbjct: 131  QAVYAEKVKNFAKENDIKIITIDTPPEG-CMHFSELTQADESEIPPVKINPDDVVALPYS 189

Query: 900  SGTTGLPKGVMLSHKNIVTCVAQKVDGEIPNFHVGRRDLTVCVLPLFHIFSLIMVLL-NM 724
            SGTTGLPKGVML+HK++VT VAQ+VDGE PN +   +D+ +CVLPLFHI+SL  VLL ++
Sbjct: 190  SGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSL 249

Query: 723  RVGAAVLIVPKFEINLLMEQIQKYRVTIAPFVXXXXXXXXXXXXXXAVFDLSSVRRVICG 544
            RVGAA+LI+ KFEI  LME +QKY+VTIAP V                +DLSS+R V+ G
Sbjct: 250  RVGAAILIMQKFEIVTLMELVQKYKVTIAPLVPPIVLAIAKTPDVDK-YDLSSIRTVMSG 308

Query: 543  AAPMDKKLEGSAKAKLPNATIGQGYGMTEAG-VISMCLGFAXXXXXXXPGSCGTVIRNYR 367
            AAPM K+LE + +A+LPNA +GQGYGMTEAG V++M L FA        GSCGTV+RN  
Sbjct: 309  AAPMGKELEDAVRARLPNAKLGQGYGMTEAGPVLAMNLAFAKQACETKSGSCGTVVRNAE 368

Query: 366  MKIVDPSTGASLSRNQTGEIRVRGDAVMKGYYNDREATRRTIDNDGWLHTGDIGYVDDDD 187
            MKIV+P TGASL RNQ+GEI +RG  +MKGY ND EAT+RTID DGWLHTGD+GY+DDDD
Sbjct: 369  MKIVNPDTGASLPRNQSGEICIRGSQIMKGYLNDPEATQRTIDKDGWLHTGDVGYIDDDD 428

Query: 186  EVFIVDRLKELIKYKGFHVAPAELEALLIAHPSISDAAVVPMLDEAAGEVPVAFVVPANG 7
            E+FIVDRLKELIKYKGF VAPAELEA+LI HP+I DAAVVPM DEAAGEVPVAFVV +NG
Sbjct: 429  ELFIVDRLKELIKYKGFQVAPAELEAMLIGHPNIFDAAVVPMKDEAAGEVPVAFVVRSNG 488

Query: 6    S 4
            S
Sbjct: 489  S 489