BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00027167
         (2025 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|OVA19386.1| DNA topoisomerase II [Macleaya cordata]                941   0.0  
ref|XP_010241214.1| PREDICTED: DNA topoisomerase 2-like isoform ...   924   0.0  
ref|XP_010241213.1| PREDICTED: DNA topoisomerase 2-like isoform ...   924   0.0  
emb|CDP07797.1| unnamed protein product [Coffea canephora]            915   0.0  
ref|XP_002274267.1| PREDICTED: DNA topoisomerase 2 [Vitis vinifera]   915   0.0  
ref|XP_020703085.1| DNA topoisomerase 2-like [Dendrobium catenat...   906   0.0  
ref|XP_016470682.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...   907   0.0  
emb|CBI24922.3| unnamed protein product, partial [Vitis vinifera]     908   0.0  
ref|XP_008779357.2| PREDICTED: DNA topoisomerase 2 [Phoenix dact...   905   0.0  
ref|XP_009800897.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...   906   0.0  
ref|XP_019258358.1| PREDICTED: DNA topoisomerase 2 isoform X2 [N...   904   0.0  
ref|XP_019258357.1| PREDICTED: DNA topoisomerase 2 isoform X1 [N...   904   0.0  
ref|XP_018621925.1| PREDICTED: DNA topoisomerase 2 isoform X2 [N...   904   0.0  
ref|XP_016508302.1| PREDICTED: DNA topoisomerase 2-like isoform ...   904   0.0  
ref|XP_018621924.1| PREDICTED: DNA topoisomerase 2 isoform X1 [N...   904   0.0  
ref|XP_016508301.1| PREDICTED: DNA topoisomerase 2-like isoform ...   904   0.0  
ref|XP_020693654.1| DNA topoisomerase 2 [Dendrobium catenatum] >...   903   0.0  
gb|OAY66581.1| DNA topoisomerase 2 [Ananas comosus]                   902   0.0  
ref|XP_010920068.1| PREDICTED: DNA topoisomerase 2 [Elaeis guine...   900   0.0  
ref|XP_006345427.1| PREDICTED: DNA topoisomerase 2 isoform X3 [S...   899   0.0  

>gb|OVA19386.1| DNA topoisomerase II [Macleaya cordata]
          Length = 1515

 Score =  941 bits (2432), Expect = 0.0
 Identities = 456/607 (75%), Positives = 528/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQSI +IL L++GK Y+SV+SLR
Sbjct: 496  MAGISVVGRNYYGVFPLRGKLLNVREASHKQIMENAEIQSIKQILGLQHGKEYDSVKSLR 555

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT + G+V+SFY
Sbjct: 556  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLIEFITPIVKATHKNGKVMSFY 615

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            S+PEYE WK+SL GN SGWSIKYYKGLGTSTSKEGKEYF+DLEKH+KDF+W+D++D DAI
Sbjct: 616  SMPEYEQWKESLGGNASGWSIKYYKGLGTSTSKEGKEYFKDLEKHRKDFMWVDEQDGDAI 675

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            +LAFSK KI+ARK WL+++EPGTYLDQKEK IKYSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 676  DLAFSKKKIEARKNWLRQFEPGTYLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG 735

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKVAQ SGYVSEHSAYHHGEQSLA TI+GMAQDY+GSNNI
Sbjct: 736  LKPGQRKILFCSFKRNFVKEAKVAQFSGYVSEHSAYHHGEQSLAGTIIGMAQDYVGSNNI 795

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTRHQGGKDHASARYIYT LSPITRFLF K+DD LL YLNEDGQS+EPTWY
Sbjct: 796  NLLQPNGQFGTRHQGGKDHASARYIYTRLSPITRFLFPKDDDILLDYLNEDGQSIEPTWY 855

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VPVIP VLVNG+EGIGTGWS YIPNYNPRDIV NV+  LN EPMEPM PWYKGFKG IE 
Sbjct: 856  VPVIPTVLVNGSEGIGTGWSCYIPNYNPRDIVENVKRLLNGEPMEPMDPWYKGFKGRIEK 915

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+ G SY V GIIEEVN+TT+RI+ELPVR+ T++YK+FL+ +++   + KE FIKD+
Sbjct: 916  TATKEGGVSYTVSGIIEEVNETTLRISELPVRRWTQDYKEFLESIMTGNDKIKEPFIKDY 975

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            RE NDD TVHFE+ +SE+NL MAK+EGL++KFKL+TT+STSNMHLF+PKGV+ KY +PE+
Sbjct: 976  REHNDDTTVHFEVILSEENLVMAKQEGLLKKFKLTTTVSTSNMHLFDPKGVIKKYDNPEQ 1035

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LRLEFYEKRK  +L           NKVRFI  V++G+II+ NRKR+DL  EL
Sbjct: 1036 ILEEFFHLRLEFYEKRKRVLLENLELELLKLDNKVRFILGVVKGEIIVSNRKRADLFLEL 1095

Query: 2005 KQKNFTP 2025
            +QK FTP
Sbjct: 1096 QQKGFTP 1102



 Score = 66.6 bits (161), Expect = 2e-07
 Identities = 38/84 (45%), Positives = 47/84 (55%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVE+L+ W  +                ITGI+KLEDAN+AG   +  CTLILTEG
Sbjct: 429 VAKSGVVESLLSWADFKQSKDLKKTDGAKRQRITGITKLEDANDAGGRNSDKCTLILTEG 488

Query: 182 DSAKALAVRWLGYLLWGETVMGCF 253
           DSAKALA+   G  + G    G F
Sbjct: 489 DSAKALAM--AGISVVGRNYYGVF 510


>ref|XP_010241214.1| PREDICTED: DNA topoisomerase 2-like isoform X2 [Nelumbo nucifera]
          Length = 1503

 Score =  924 bits (2389), Expect = 0.0
 Identities = 452/607 (74%), Positives = 520/607 (85%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA + QI  N EIQ+I +IL L++GK Y+SV+SLR
Sbjct: 469  MAGISVVGRNNYGVFPLRGKLLNVREATHKQIMDNAEIQNIKQILGLQHGKEYDSVKSLR 528

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT + G+VLSFY
Sbjct: 529  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATHKNGKVLSFY 588

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            S+PEYE+WK  L GN SGWSIKYYKGLGTSTSKEGKEYF+DL KHKKDFLW+D +D DAI
Sbjct: 589  SMPEYESWKX-LGGNASGWSIKYYKGLGTSTSKEGKEYFKDLHKHKKDFLWVDDRDGDAI 647

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT LDQKEK IKYSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 648  ELAFSKKKIEARKNWLRQFEPGTCLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG 707

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRN IKEAKVAQ SGYVSEHSAYHHGEQSL +TI+GMAQDY+GSNNI
Sbjct: 708  LKPGQRKILFCAFKRNLIKEAKVAQFSGYVSEHSAYHHGEQSLMSTIIGMAQDYVGSNNI 767

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NL QPNGQFGTRHQGGKDHASARYIYT LSPITRFLF K DD LL YLNEDGQS+EPTWY
Sbjct: 768  NLFQPNGQFGTRHQGGKDHASARYIYTRLSPITRFLFPKGDDILLDYLNEDGQSIEPTWY 827

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            +P+IP VLVNG+EGIGTGWS+YIPNYNPRDIVAN++  LN EPMEPM PWYKGFKG IE 
Sbjct: 828  MPIIPTVLVNGSEGIGTGWSSYIPNYNPRDIVANIKRLLNGEPMEPMDPWYKGFKGLIEK 887

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG SY V GIIEE+N+TT+RITELP+R+ T++YK+FL+ +++   + KE FIKD+
Sbjct: 888  TATKEAGSSYTVSGIIEEINETTLRITELPIRRWTQDYKEFLESIMTGNDKIKEPFIKDY 947

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            +E NDD TVHFEI +SE+NL MAK+EGL++KFKL+TTISTSNMHLF+PKG + KY +PE+
Sbjct: 948  KEHNDDTTVHFEIILSEENLMMAKQEGLMKKFKLTTTISTSNMHLFDPKGTIKKYDNPEQ 1007

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LRLEFYEKRK   L           NKVRFI  V++G+II+ NRKR DL  EL
Sbjct: 1008 ILEEFFHLRLEFYEKRKKTQLDNLELDLLKLDNKVRFILGVVKGEIIVSNRKRVDLFLEL 1067

Query: 2005 KQKNFTP 2025
            +QK FTP
Sbjct: 1068 QQKGFTP 1074



 Score = 68.6 bits (166), Expect = 4e-08
 Identities = 35/68 (51%), Positives = 43/68 (63%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVENL+ W  +                ITGI+KLEDAN+AG   ++ CTLILTEG
Sbjct: 402 VAKSGVVENLLSWADFKQSKDLKKTDGAKRQRITGITKLEDANDAGGRNSEKCTLILTEG 461

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 462 DSAKALAM 469


>ref|XP_010241213.1| PREDICTED: DNA topoisomerase 2-like isoform X1 [Nelumbo nucifera]
          Length = 1504

 Score =  924 bits (2389), Expect = 0.0
 Identities = 452/607 (74%), Positives = 520/607 (85%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA + QI  N EIQ+I +IL L++GK Y+SV+SLR
Sbjct: 469  MAGISVVGRNNYGVFPLRGKLLNVREATHKQIMDNAEIQNIKQILGLQHGKEYDSVKSLR 528

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT + G+VLSFY
Sbjct: 529  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATHKNGKVLSFY 588

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            S+PEYE+WK  L GN SGWSIKYYKGLGTSTSKEGKEYF+DL KHKKDFLW+D +D DAI
Sbjct: 589  SMPEYESWKX-LGGNASGWSIKYYKGLGTSTSKEGKEYFKDLHKHKKDFLWVDDRDGDAI 647

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT LDQKEK IKYSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 648  ELAFSKKKIEARKNWLRQFEPGTCLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG 707

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRN IKEAKVAQ SGYVSEHSAYHHGEQSL +TI+GMAQDY+GSNNI
Sbjct: 708  LKPGQRKILFCAFKRNLIKEAKVAQFSGYVSEHSAYHHGEQSLMSTIIGMAQDYVGSNNI 767

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NL QPNGQFGTRHQGGKDHASARYIYT LSPITRFLF K DD LL YLNEDGQS+EPTWY
Sbjct: 768  NLFQPNGQFGTRHQGGKDHASARYIYTRLSPITRFLFPKGDDILLDYLNEDGQSIEPTWY 827

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            +P+IP VLVNG+EGIGTGWS+YIPNYNPRDIVAN++  LN EPMEPM PWYKGFKG IE 
Sbjct: 828  MPIIPTVLVNGSEGIGTGWSSYIPNYNPRDIVANIKRLLNGEPMEPMDPWYKGFKGLIEK 887

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG SY V GIIEE+N+TT+RITELP+R+ T++YK+FL+ +++   + KE FIKD+
Sbjct: 888  TATKEAGSSYTVSGIIEEINETTLRITELPIRRWTQDYKEFLESIMTGNDKIKEPFIKDY 947

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            +E NDD TVHFEI +SE+NL MAK+EGL++KFKL+TTISTSNMHLF+PKG + KY +PE+
Sbjct: 948  KEHNDDTTVHFEIILSEENLMMAKQEGLMKKFKLTTTISTSNMHLFDPKGTIKKYDNPEQ 1007

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LRLEFYEKRK   L           NKVRFI  V++G+II+ NRKR DL  EL
Sbjct: 1008 ILEEFFHLRLEFYEKRKKTQLDNLELDLLKLDNKVRFILGVVKGEIIVSNRKRVDLFLEL 1067

Query: 2005 KQKNFTP 2025
            +QK FTP
Sbjct: 1068 QQKGFTP 1074



 Score = 68.6 bits (166), Expect = 4e-08
 Identities = 35/68 (51%), Positives = 43/68 (63%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVENL+ W  +                ITGI+KLEDAN+AG   ++ CTLILTEG
Sbjct: 402 VAKSGVVENLLSWADFKQSKDLKKTDGAKRQRITGITKLEDANDAGGRNSEKCTLILTEG 461

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 462 DSAKALAM 469


>emb|CDP07797.1| unnamed protein product [Coffea canephora]
          Length = 1486

 Score =  915 bits (2365), Expect = 0.0
 Identities = 439/607 (72%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQ I +IL L++GK Y+SV+SLR
Sbjct: 466  MAGISVVGRNFYGVFPLRGKLLNVREASHKQIMDNAEIQYIKQILGLQHGKVYDSVKSLR 525

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT + G+VLSFY
Sbjct: 526  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATHKNGRVLSFY 585

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+WK+SL GN +GWSIKYYKGLGTSTSKEGKEYF+DL KHKKDF+W+D  D +AI
Sbjct: 586  TMPEYESWKESLSGNATGWSIKYYKGLGTSTSKEGKEYFKDLGKHKKDFIWVDDNDGEAI 645

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGTYLDQKEK IKYSDF++KELI FS+ADL+RSIPSMVDG
Sbjct: 646  ELAFSKKKIEARKNWLRQFEPGTYLDQKEKLIKYSDFINKELILFSLADLQRSIPSMVDG 705

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKVAQ SGYVSEHSAYHHGEQSLA+ I+GMAQD++GSNNI
Sbjct: 706  LKPGQRKILFCSFKRNFVKEAKVAQFSGYVSEHSAYHHGEQSLASAIIGMAQDFVGSNNI 765

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+QGGKDHASARY+YT LSPITR LF K+DD LL YLNEDGQS+EPTWY
Sbjct: 766  NLLQPNGQFGTRNQGGKDHASARYVYTRLSPITRSLFPKDDDILLDYLNEDGQSIEPTWY 825

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            +P+IPMVLVNG+EGIGTGWS+YIPNYNPRDI+ N+R  LN+EPMEPM PWYKGF+GTIE 
Sbjct: 826  MPIIPMVLVNGSEGIGTGWSSYIPNYNPRDIIGNIRRLLNNEPMEPMEPWYKGFRGTIEK 885

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG SY V GI+EEV++TT+R+TELPVRK T++YK+FL+ ++    + K+ FIK+F
Sbjct: 886  TATKEAGASYTVSGIVEEVDETTIRVTELPVRKWTQDYKEFLESIMMGNEKVKDPFIKEF 945

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R+  DD TV+ +I MSE+NL MAK+EGL++KFKL+TTISTSNMHLF+ +G++ K+ +PE+
Sbjct: 946  RDHTDDTTVYLDIIMSEENLLMAKQEGLLKKFKLTTTISTSNMHLFDSQGIIKKFDNPEQ 1005

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LRLEFYEKRK  +L           NKVRFI  V++G II+ NRKR+DL  EL
Sbjct: 1006 ILEEFFHLRLEFYEKRKKVLLDNLQLELLKLDNKVRFILAVVEGSIIVSNRKRADLFLEL 1065

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1066 KEKGFTP 1072



 Score = 68.2 bits (165), Expect = 6e-08
 Identities = 39/84 (46%), Positives = 47/84 (55%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVENL+ W  +                ITGI+KLEDAN+AG   +  CTLILTEG
Sbjct: 399 VAKSGVVENLLSWADFKQSKDLKKTDGTKRQRITGITKLEDANDAGGKNSDRCTLILTEG 458

Query: 182 DSAKALAVRWLGYLLWGETVMGCF 253
           DSAKALA+   G  + G    G F
Sbjct: 459 DSAKALAM--AGISVVGRNFYGVF 480


>ref|XP_002274267.1| PREDICTED: DNA topoisomerase 2 [Vitis vinifera]
          Length = 1489

 Score =  915 bits (2364), Expect = 0.0
 Identities = 447/608 (73%), Positives = 519/608 (85%), Gaps = 2/608 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVR+A   QI  N EIQ+I +IL L+ GK Y+S++SLR
Sbjct: 465  MAGISVVGRNHYGVFPLRGKLLNVRDAQKKQILENAEIQNIKQILGLQQGKEYDSIKSLR 524

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT R G+VLSFY
Sbjct: 525  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATHRNGRVLSFY 584

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            S+PEYE+WK+SL GN SGWSIKYYKGLGTSTSKEGKEYF+DL KHKKDF+W+D+KD DAI
Sbjct: 585  SMPEYESWKESLGGNASGWSIKYYKGLGTSTSKEGKEYFKDLGKHKKDFMWVDEKDGDAI 644

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ RK WL++++PGTYLDQKEK IKYSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 645  ELAFSKKKIEERKNWLRQFQPGTYLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG 704

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNFIKEAK+AQ SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNNI
Sbjct: 705  LKPGQRKILFCSFKRNFIKEAKIAQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNI 764

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLL PNGQFGTRHQGGKDHASARYIYT LSPITRFLF K+DD LL YLNEDGQS+EPTWY
Sbjct: 765  NLLLPNGQFGTRHQGGKDHASARYIYTRLSPITRFLFPKDDDILLDYLNEDGQSIEPTWY 824

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP IPMVLVNG+EGIGTGWS+ IPNYNPRDI+ANVR  LN E MEPM PWY+GF+GTIE 
Sbjct: 825  VPTIPMVLVNGSEGIGTGWSSSIPNYNPRDIIANVRRLLNGEMMEPMDPWYRGFRGTIEK 884

Query: 1465 TN-TKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKD 1641
            T+ TK     Y VKGIIEEVN+TT+RI+ELPVRK T++YK+FL+ +++   + K+ FIKD
Sbjct: 885  TDPTKQESVGYTVKGIIEEVNETTLRISELPVRKWTQDYKEFLESIMTGNDKIKDPFIKD 944

Query: 1642 FREFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPE 1821
            ++E NDD TVHFE+ MSE+NL MAK+EGL++KFKL+T ISTSNMHLF+  G + KY +PE
Sbjct: 945  YKEHNDDTTVHFEVIMSEENLLMAKQEGLLQKFKLTTKISTSNMHLFDSNGTIKKYETPE 1004

Query: 1822 KIIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDE 2001
            +I+EEF  LRLEFYEKRK  +L           NKVRFI  V++G+II+ NRKR+DL  E
Sbjct: 1005 QILEEFFHLRLEFYEKRKRVLLDNLELELLKLENKVRFILGVVRGEIIVSNRKRADLFHE 1064

Query: 2002 LKQKNFTP 2025
            L QK FTP
Sbjct: 1065 LHQKGFTP 1072



 Score = 67.0 bits (162), Expect = 1e-07
 Identities = 34/68 (50%), Positives = 43/68 (63%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVE+L+ W  Y                +TGI+KLEDAN+AG   ++ CTLILTEG
Sbjct: 398 VSKSGVVESLLSWADYKQNKDLKKTDGTKRQRLTGITKLEDANDAGGRNSEKCTLILTEG 457

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 458 DSAKALAM 465


>ref|XP_020703085.1| DNA topoisomerase 2-like [Dendrobium catenatum]
 gb|PKU64218.1| DNA topoisomerase 2 [Dendrobium catenatum]
          Length = 1268

 Score =  906 bits (2341), Expect = 0.0
 Identities = 437/607 (71%), Positives = 518/607 (85%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQ+I +IL L+ GK Y SV+ LR
Sbjct: 273  MAGISVVGRNNYGVFPLRGKLLNVREASHKQIMENAEIQNIKQILGLQQGKQYESVKGLR 332

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT R G+VLSFY
Sbjct: 333  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATYRTGKVLSFY 392

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+WK+SL GN SGWSIKYYKGLGTSTSKEGKEYFQDL+KHKKDF+W D++D +AI
Sbjct: 393  TMPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGKEYFQDLQKHKKDFVWFDEQDGEAI 452

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGTYLDQ EK I YSDFV+KELI FS+ADL+RSIPSM+DG
Sbjct: 453  ELAFSKKKIEARKNWLRQFEPGTYLDQNEKLISYSDFVNKELILFSMADLQRSIPSMLDG 512

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAK++Q SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNNI
Sbjct: 513  LKPGQRKILFCSFKRNFVKEAKISQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNI 572

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTRHQGGKDHASARYIYT LSP+TRFLF K DD +L Y++EDGQS+EP WY
Sbjct: 573  NLLQPNGQFGTRHQGGKDHASARYIYTKLSPMTRFLFPKEDDLILDYMSEDGQSIEPAWY 632

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP+IPMVLVNG+EGIGTGWS+YIPNYNPRDIV NVR  LNDEPM PM PWY+GFKG IE 
Sbjct: 633  VPIIPMVLVNGSEGIGTGWSSYIPNYNPRDIVKNVRRLLNDEPMLPMDPWYRGFKGLIEK 692

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T  K+AG +Y V GIIEE +  T+ ITELPVR+ T++YK+FL+ L++   + K+ FIKD+
Sbjct: 693  TAVKEAGATYTVTGIIEESDSNTLTITELPVRRWTQDYKEFLESLVTGTDKVKDPFIKDY 752

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            RE+NDD TVHFE+ +SE+NL MAK+EGL +KFKLSTTISTSNMHLF+ KGV+ KY +PE+
Sbjct: 753  REYNDDTTVHFEVTLSEQNLIMAKQEGLEKKFKLSTTISTSNMHLFDHKGVIKKYDTPEQ 812

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LR+ +YEKRK  +L           NKVRFI  V++G+II+ NRKR++L  EL
Sbjct: 813  ILEEFFHLRITYYEKRKKALLDNLELDLLKLDNKVRFILGVVKGQIIVNNRKRAELFLEL 872

Query: 2005 KQKNFTP 2025
            ++K FTP
Sbjct: 873  QEKGFTP 879



 Score = 62.4 bits (150), Expect = 3e-06
 Identities = 31/68 (45%), Positives = 42/68 (61%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +G+VE+L+ W  +                I+GI KL+DAN+AG   ++ CTLILTEG
Sbjct: 206 VAKSGIVESLLSWADFKQNKELKKTDGTKRQKISGIVKLDDANDAGGKNSEKCTLILTEG 265

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 266 DSAKALAM 273


>ref|XP_016470682.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Nicotiana
            tabacum]
          Length = 1438

 Score =  907 bits (2345), Expect = 0.0
 Identities = 427/607 (70%), Positives = 527/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y+SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYDSVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQS+EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQSIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEEVN+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEEVNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ +SE+NL +A++EGL++KFKL+TTISTSNMHLF+ KG + KY +PE 
Sbjct: 941  RAYGDENSVCFEVILSEENLILAQQEGLLKKFKLATTISTSNMHLFDSKGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKRALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>emb|CBI24922.3| unnamed protein product, partial [Vitis vinifera]
          Length = 1452

 Score =  908 bits (2346), Expect = 0.0
 Identities = 444/604 (73%), Positives = 516/604 (85%), Gaps = 2/604 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVR+A   QI  N EIQ+I +IL L+ GK Y+S++SLR
Sbjct: 465  MAGISVVGRNHYGVFPLRGKLLNVRDAQKKQILENAEIQNIKQILGLQQGKEYDSIKSLR 524

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT R G+VLSFY
Sbjct: 525  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATHRNGRVLSFY 584

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            S+PEYE+WK+SL GN SGWSIKYYKGLGTSTSKEGKEYF+DL KHKKDF+W+D+KD DAI
Sbjct: 585  SMPEYESWKESLGGNASGWSIKYYKGLGTSTSKEGKEYFKDLGKHKKDFMWVDEKDGDAI 644

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ RK WL++++PGTYLDQKEK IKYSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 645  ELAFSKKKIEERKNWLRQFQPGTYLDQKEKLIKYSDFVNKELILFSMADLQRSIPSMVDG 704

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNFIKEAK+AQ SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNNI
Sbjct: 705  LKPGQRKILFCSFKRNFIKEAKIAQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNI 764

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLL PNGQFGTRHQGGKDHASARYIYT LSPITRFLF K+DD LL YLNEDGQS+EPTWY
Sbjct: 765  NLLLPNGQFGTRHQGGKDHASARYIYTRLSPITRFLFPKDDDILLDYLNEDGQSIEPTWY 824

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP IPMVLVNG+EGIGTGWS+ IPNYNPRDI+ANVR  LN E MEPM PWY+GF+GTIE 
Sbjct: 825  VPTIPMVLVNGSEGIGTGWSSSIPNYNPRDIIANVRRLLNGEMMEPMDPWYRGFRGTIEK 884

Query: 1465 TN-TKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKD 1641
            T+ TK     Y VKGIIEEVN+TT+RI+ELPVRK T++YK+FL+ +++   + K+ FIKD
Sbjct: 885  TDPTKQESVGYTVKGIIEEVNETTLRISELPVRKWTQDYKEFLESIMTGNDKIKDPFIKD 944

Query: 1642 FREFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPE 1821
            ++E NDD TVHFE+ MSE+NL MAK+EGL++KFKL+T ISTSNMHLF+  G + KY +PE
Sbjct: 945  YKEHNDDTTVHFEVIMSEENLLMAKQEGLLQKFKLTTKISTSNMHLFDSNGTIKKYETPE 1004

Query: 1822 KIIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDE 2001
            +I+EEF  LRLEFYEKRK  +L           NKVRFI  V++G+II+ NRKR+DL  E
Sbjct: 1005 QILEEFFHLRLEFYEKRKRVLLDNLELELLKLENKVRFILGVVRGEIIVSNRKRADLFHE 1064

Query: 2002 LKQK 2013
            L QK
Sbjct: 1065 LHQK 1068



 Score = 67.0 bits (162), Expect = 1e-07
 Identities = 34/68 (50%), Positives = 43/68 (63%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVVE+L+ W  Y                +TGI+KLEDAN+AG   ++ CTLILTEG
Sbjct: 398 VSKSGVVESLLSWADYKQNKDLKKTDGTKRQRLTGITKLEDANDAGGRNSEKCTLILTEG 457

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 458 DSAKALAM 465


>ref|XP_008779357.2| PREDICTED: DNA topoisomerase 2 [Phoenix dactylifera]
          Length = 1378

 Score =  905 bits (2338), Expect = 0.0
 Identities = 433/608 (71%), Positives = 518/608 (85%), Gaps = 2/608 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQ+I +IL L++GK Y+S + LR
Sbjct: 369  MAGISVVGRNYYGVFPLRGKLLNVREASHKQIMENAEIQNIKQILGLQHGKEYDSTKGLR 428

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKATRAGQ--VLSF 561
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+F++EFITPI+KAT +    VL F
Sbjct: 429  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFMVEFITPIVKATHSKNKTVLPF 488

Query: 562  YSIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDA 741
            YS+PEYEAWK+ L GN SGWSIKYYKGLGTSTSKEGKEYF D++KHKKDF+W+D +D DA
Sbjct: 489  YSMPEYEAWKERLGGNASGWSIKYYKGLGTSTSKEGKEYFHDIDKHKKDFIWVDDQDGDA 548

Query: 742  IELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVD 921
            IELAFSK KI+ARK WL+ +E GTYLDQ++KHIKYSDFV+KELI FS+ADL+RSIPS VD
Sbjct: 549  IELAFSKKKIEARKNWLRHFETGTYLDQRQKHIKYSDFVNKELILFSMADLQRSIPSTVD 608

Query: 922  GFKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNN 1101
            G KPGQRKILF  FKRNF+KEAKVAQ SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNN
Sbjct: 609  GLKPGQRKILFCSFKRNFVKEAKVAQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNN 668

Query: 1102 INLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTW 1281
            INLLQPNGQFGTRHQGGKDHASARYIYT LSP+TRFLF K+DD LL YLNEDGQS+EPTW
Sbjct: 669  INLLQPNGQFGTRHQGGKDHASARYIYTRLSPLTRFLFPKDDDMLLDYLNEDGQSIEPTW 728

Query: 1282 YVPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIE 1461
            Y+P+IP+VLVNG+EGIGTGWS+++PNYNPRDIVANVR  LNDEPM+PM PWY+GFKG IE
Sbjct: 729  YMPIIPLVLVNGSEGIGTGWSSFVPNYNPRDIVANVRRLLNDEPMQPMDPWYRGFKGLIE 788

Query: 1462 STNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKD 1641
             + TK+AG +Y + G+IEEV+ TT+RITELP+R+ T++YK+FL+ +++   + KE FIKD
Sbjct: 789  KSATKEAGVTYTITGLIEEVDSTTLRITELPIRRWTQDYKEFLESMMTGNDKIKEPFIKD 848

Query: 1642 FREFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPE 1821
            +RE NDD  VHFEI ++E+ L MAKEEGL  KFKL+TTIST+NMHLF+ +GV+ KY +PE
Sbjct: 849  YREHNDDINVHFEIILTEEKLKMAKEEGLERKFKLTTTISTTNMHLFDSEGVIKKYDTPE 908

Query: 1822 KIIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDE 2001
            +I+E+F  LRLE+Y KRK  +L           NKVRFI  V++G+II+ NRKR+DL  E
Sbjct: 909  QILEDFFHLRLEYYAKRKKVLLDNLEMDLLKLDNKVRFILGVVRGEIIVSNRKRADLFLE 968

Query: 2002 LKQKNFTP 2025
            L+QK FTP
Sbjct: 969  LQQKGFTP 976



 Score = 64.7 bits (156), Expect = 7e-07
 Identities = 37/84 (44%), Positives = 46/84 (54%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVV +L+ W  +                ITGI KLEDAN+AG   ++ CTLILTEG
Sbjct: 302 VAKSGVVNSLLSWADFKQSKELKKTDGTKRQRITGIPKLEDANDAGGRNSEKCTLILTEG 361

Query: 182 DSAKALAVRWLGYLLWGETVMGCF 253
           DSAKALA+   G  + G    G F
Sbjct: 362 DSAKALAM--AGISVVGRNYYGVF 383


>ref|XP_009800897.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Nicotiana
            sylvestris]
          Length = 1474

 Score =  906 bits (2342), Expect = 0.0
 Identities = 426/607 (70%), Positives = 527/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y+SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYDSVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQS+EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQSIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEE+N+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEELNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ +SE+NL +A++EGL++KFKL+TTISTSNMHLF+ KG + KY +PE 
Sbjct: 941  RAYGDENSVCFEVILSEENLILAQQEGLLKKFKLATTISTSNMHLFDSKGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKRALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_019258358.1| PREDICTED: DNA topoisomerase 2 isoform X2 [Nicotiana attenuata]
 gb|OIT40573.1| dna topoisomerase 2 [Nicotiana attenuata]
          Length = 1468

 Score =  904 bits (2336), Expect = 0.0
 Identities = 425/607 (70%), Positives = 526/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y+SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYDSVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL   +SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGATSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQS+EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQSIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPM+PM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMKPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEE+N+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEELNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+ KG + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSKGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKRALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLFEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_019258357.1| PREDICTED: DNA topoisomerase 2 isoform X1 [Nicotiana attenuata]
          Length = 1469

 Score =  904 bits (2336), Expect = 0.0
 Identities = 425/607 (70%), Positives = 526/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y+SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYDSVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL   +SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGATSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQS+EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQSIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPM+PM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMKPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEE+N+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEELNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+ KG + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSKGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKRALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLFEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_018621925.1| PREDICTED: DNA topoisomerase 2 isoform X2 [Nicotiana tomentosiformis]
          Length = 1481

 Score =  904 bits (2336), Expect = 0.0
 Identities = 426/607 (70%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYESVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQ +EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQYIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEEVN+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEEVNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+  G + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSNGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKKALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_016508302.1| PREDICTED: DNA topoisomerase 2-like isoform X2 [Nicotiana tabacum]
          Length = 1481

 Score =  904 bits (2336), Expect = 0.0
 Identities = 426/607 (70%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYESVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQ +EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQYIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEEVN+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEEVNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+  G + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSNGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKKALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_018621924.1| PREDICTED: DNA topoisomerase 2 isoform X1 [Nicotiana tomentosiformis]
          Length = 1482

 Score =  904 bits (2336), Expect = 0.0
 Identities = 426/607 (70%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYESVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQ +EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQYIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEEVN+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEEVNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+  G + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSNGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKKALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_016508301.1| PREDICTED: DNA topoisomerase 2-like isoform X1 [Nicotiana tabacum]
          Length = 1482

 Score =  904 bits (2336), Expect = 0.0
 Identities = 426/607 (70%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI++I KIL L+ GK Y SV+SLR
Sbjct: 461  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIEAIKKILGLQTGKEYESVKSLR 520

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++LSFY
Sbjct: 521  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLIEFITPIVKATHKSGKILSFY 580

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+W++SL  N+SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 581  TMPEYESWRKSLGANSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 640

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 641  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 700

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 701  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 760

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K DD +L YLNEDGQ +EPTWY
Sbjct: 761  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKEDDTILDYLNEDGQYIEPTWY 820

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP++PMVL+NG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDEPMEPM PWYKGFKGTIE 
Sbjct: 821  VPIVPMVLINGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDEPMEPMDPWYKGFKGTIEK 880

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+AG +Y V GIIEEVN+TT+RI+ELPVR+ TE+YKQFL+ +     + K+ FIK+ 
Sbjct: 881  TATKEAGATYTVTGIIEEVNETTLRISELPVRRWTEDYKQFLESMTVSNDKAKDPFIKEV 940

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+  G + KY +PE 
Sbjct: 941  RAYGDENSVCFEVIMSEENLILAQQEGLLKKFKLATTISTSNMHLFDSNGKIKKYDNPED 1000

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKV+FI  V++G+II+ NRKR+DLL EL
Sbjct: 1001 ILEEFYHVRLEYYEKRKKALLEILELELLRIENKVKFILGVVKGEIIVNNRKRADLLLEL 1060

Query: 2005 KQKNFTP 2025
            K+K FTP
Sbjct: 1061 KEKGFTP 1067


>ref|XP_020693654.1| DNA topoisomerase 2 [Dendrobium catenatum]
 gb|PKU72193.1| DNA topoisomerase 2 [Dendrobium catenatum]
          Length = 1466

 Score =  903 bits (2333), Expect = 0.0
 Identities = 436/607 (71%), Positives = 517/607 (85%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQ+I +IL L+ GK Y SV+ LR
Sbjct: 471  MAGISVVGRNNYGVFPLRGKLLNVREASHKQIMENAEIQNIKQILGLQQGKEYESVKGLR 530

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+FL+EFITPI+KAT R G+VLSFY
Sbjct: 531  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFLVEFITPIVKATYRTGKVLSFY 590

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYE+WK+SL GN SGWSIKYYKGLGTSTSKEGKEYFQDL+KHKKDF+W D++D +AI
Sbjct: 591  TMPEYESWKESLGGNASGWSIKYYKGLGTSTSKEGKEYFQDLQKHKKDFVWFDEQDGEAI 650

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGTYLDQ EK I YSDFV+KELI FS+ADL+RSIPSMVDG
Sbjct: 651  ELAFSKKKIEARKNWLRQFEPGTYLDQNEKLISYSDFVNKELILFSMADLQRSIPSMVDG 710

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAK++Q SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNNI
Sbjct: 711  LKPGQRKILFCSFKRNFVKEAKISQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNI 770

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTRHQGGKDHASARYIYT LSP+TRFLF K DD +L Y++EDGQS+EP WY
Sbjct: 771  NLLQPNGQFGTRHQGGKDHASARYIYTKLSPMTRFLFPKEDDLILDYMSEDGQSIEPAWY 830

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            VP+IPMVLVNG+EGIGTGWS+YIPNYNPRDIV N+R  LNDEPM PM PWY+GFKG IE 
Sbjct: 831  VPIIPMVLVNGSEGIGTGWSSYIPNYNPRDIVKNLRRLLNDEPMLPMDPWYRGFKGLIEK 890

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T  K+A  +Y V GIIEE +  T+ ITELPVR+ T++YK+FL+ L++   + KE FIKD+
Sbjct: 891  TAVKEACATYTVTGIIEESDSNTLTITELPVRRWTQDYKEFLESLVTGTDKVKEPFIKDY 950

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            RE+NDD TVHFE+ +S++NL MAK+EGL +KFKLSTTISTSNMHLF+ KGV+ KY +PE+
Sbjct: 951  REYNDDTTVHFEVTLSDQNLIMAKQEGLEKKFKLSTTISTSNMHLFDHKGVIKKYDTPEQ 1010

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  LR+ +YEKRK  +L           NKVRFI  V++G+II+ NRKR++L  EL
Sbjct: 1011 ILEEFFHLRITYYEKRKKALLDNLELDLLKLDNKVRFILGVVKGQIIVNNRKRAELFLEL 1070

Query: 2005 KQKNFTP 2025
            ++K FTP
Sbjct: 1071 QEKGFTP 1077



 Score = 62.4 bits (150), Expect = 3e-06
 Identities = 31/68 (45%), Positives = 42/68 (61%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +G+VE+L+ W  +                I+GI KL+DAN+AG   ++ CTLILTEG
Sbjct: 404 VAKSGIVESLLSWADFKQNKELKKTDGTKRQKISGIVKLDDANDAGGKNSEKCTLILTEG 463

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 464 DSAKALAM 471


>gb|OAY66581.1| DNA topoisomerase 2 [Ananas comosus]
          Length = 1476

 Score =  902 bits (2332), Expect = 0.0
 Identities = 432/608 (71%), Positives = 520/608 (85%), Gaps = 2/608 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVR+AN+ QI  N EIQ+I  IL L++GK Y+S + LR
Sbjct: 469  MAGISVVGRNYYGVFPLRGKLLNVRDANHKQIMDNAEIQNIKLILGLQHGKQYDSTKGLR 528

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT--RAGQVLSF 561
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+F++EFITPI+KAT  R   VLSF
Sbjct: 529  YGHLMIMTDQDHDGSHIKGLLINFVHSFWPSLLKIPSFMVEFITPIVKATHNRNKTVLSF 588

Query: 562  YSIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDA 741
            YS+PEYEAWK+SL G+ +GWSIKYYKGLGTSTSKEGKEYFQD++KHKKDF+W+D++D DA
Sbjct: 589  YSMPEYEAWKESLGGSANGWSIKYYKGLGTSTSKEGKEYFQDIDKHKKDFVWVDEQDGDA 648

Query: 742  IELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVD 921
            I+LAFSK KI+ARK WL+++EPGTYLDQ+ K IKYSDFV+KELI FS+ADL+RSIPSMVD
Sbjct: 649  IDLAFSKKKIEARKNWLRQFEPGTYLDQRMKKIKYSDFVNKELILFSMADLQRSIPSMVD 708

Query: 922  GFKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNN 1101
            G KPGQRKILF  FKRNF+KEAKVAQ SGYVSEHSAYHHGEQSLA+TI+GMAQD++GSNN
Sbjct: 709  GLKPGQRKILFCSFKRNFVKEAKVAQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNN 768

Query: 1102 INLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTW 1281
            INLLQPNGQFGTR+QGGKDHASARYIYT LSP+TRFLF K+DD LL YLNEDGQS+EPTW
Sbjct: 769  INLLQPNGQFGTRNQGGKDHASARYIYTSLSPLTRFLFPKDDDILLDYLNEDGQSIEPTW 828

Query: 1282 YVPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIE 1461
            Y+P+IPMVL+NG+EGIGTGWS+++PNYNPRDI+ANVR  LNDEPM+PM PWY+GFKG IE
Sbjct: 829  YIPIIPMVLINGSEGIGTGWSSFVPNYNPRDIIANVRRLLNDEPMQPMDPWYRGFKGLIE 888

Query: 1462 STNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKD 1641
             + TK+AG +Y + GIIEEV+  T+RI ELPVR+ T++YK+FL+ L++   + KE FIKD
Sbjct: 889  KSATKEAGVTYTITGIIEEVDNATLRIMELPVRRWTQDYKEFLEALMTGNDKVKEPFIKD 948

Query: 1642 FREFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPE 1821
            +RE NDD TVHFE+ ++E+NL +A +EGL +KFKL+TTI T+NMHLF+  GV+ KY +PE
Sbjct: 949  YREHNDDTTVHFEVTLTEENLNIALQEGLEKKFKLTTTIGTTNMHLFDSNGVIKKYDTPE 1008

Query: 1822 KIIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDE 2001
            +I+E+F  LRLEFYEKRK  +L           NKVRFI  V++G+II+ NRKR++L  E
Sbjct: 1009 QILEDFFHLRLEFYEKRKKVLLDNLEMDLLKLDNKVRFILGVVRGEIIVNNRKRAELFIE 1068

Query: 2002 LKQKNFTP 2025
            L QK FTP
Sbjct: 1069 LHQKGFTP 1076



 Score = 66.6 bits (161), Expect = 2e-07
 Identities = 38/84 (45%), Positives = 46/84 (54%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVV+NL+ W  +                ITGI KLEDAN+AG   +  CTLILTEG
Sbjct: 402 VAKSGVVDNLLSWADFKQSKELKKTDGTKRQRITGIPKLEDANDAGGKNSDKCTLILTEG 461

Query: 182 DSAKALAVRWLGYLLWGETVMGCF 253
           DSAKALA+   G  + G    G F
Sbjct: 462 DSAKALAM--AGISVVGRNYYGVF 483


>ref|XP_010920068.1| PREDICTED: DNA topoisomerase 2 [Elaeis guineensis]
          Length = 1486

 Score =  900 bits (2327), Expect = 0.0
 Identities = 435/608 (71%), Positives = 516/608 (84%), Gaps = 2/608 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGR+ YGVFPLRGKLLNVREA++ QI  N EIQSI +IL L+ GK Y S + LR
Sbjct: 471  MAGISVVGRNHYGVFPLRGKLLNVREASHKQIMDNAEIQSIKQILGLQQGKEYESTKGLR 530

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT--RAGQVLSF 561
            YGH+MIMTDQDHDGSHIKGLLIN  HS WPSLLKIP+F++EFITPI+KAT  R   VL F
Sbjct: 531  YGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLKIPSFMVEFITPIVKATHNRNKNVLPF 590

Query: 562  YSIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDA 741
            YS+PEYEAWK+SL GN SGWSIKYYKGLGTSTSKEGKEYFQD+ KHKKDF+W+D +D DA
Sbjct: 591  YSMPEYEAWKESLGGNASGWSIKYYKGLGTSTSKEGKEYFQDIVKHKKDFMWVDNQDGDA 650

Query: 742  IELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVD 921
            IELAFSK KI+ARK WL+++EPGTYLDQ+EK IKYSDFV+KELI FS+ADL+RSIPSMVD
Sbjct: 651  IELAFSKKKIEARKNWLRQFEPGTYLDQREKLIKYSDFVNKELILFSMADLQRSIPSMVD 710

Query: 922  GFKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNN 1101
            G KPGQRKILF  FKRNF+KEAKVAQ +GYVSEHSAYHHGEQSLA+TI+GMAQD++GSNN
Sbjct: 711  GLKPGQRKILFCSFKRNFVKEAKVAQFAGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNN 770

Query: 1102 INLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTW 1281
            INLLQPNGQFGTRHQGGKDHASARYIYT LSP+TRFLF K+DD LL YLNEDGQ +EPTW
Sbjct: 771  INLLQPNGQFGTRHQGGKDHASARYIYTRLSPLTRFLFPKDDDILLDYLNEDGQLIEPTW 830

Query: 1282 YVPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIE 1461
            Y+P+IPMVLVNG+EGIGTGWST++PNYNPRDIVANVR  LN+EPM+PM PWY+ FKG IE
Sbjct: 831  YMPIIPMVLVNGSEGIGTGWSTFVPNYNPRDIVANVRRLLNNEPMQPMDPWYRSFKGLIE 890

Query: 1462 STNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKD 1641
             + +K+AG +Y + G+ EEV+ TT++ITELP+R+ T++YK+FL+ L++   + KE FIKD
Sbjct: 891  KSASKEAGVTYTITGLAEEVDSTTLKITELPIRRWTQDYKEFLESLMTGNDKIKEPFIKD 950

Query: 1642 FREFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPE 1821
            +RE NDD  VHFEI ++E+ L MA+EEGL +KFKL+TTIST+NMHLF+ KGV+ KY +PE
Sbjct: 951  YREQNDDVNVHFEIILTEEKLNMAREEGLEKKFKLTTTISTTNMHLFDSKGVIKKYDTPE 1010

Query: 1822 KIIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDE 2001
            +I+E+F  LRLEFY KRK  +L           NKVRFI  V++G+II+ NRKR+DL  E
Sbjct: 1011 QILEDFFHLRLEFYAKRKKALLDNLELDLLKLDNKVRFILGVVRGEIIVNNRKRADLFLE 1070

Query: 2002 LKQKNFTP 2025
            L+QK F P
Sbjct: 1071 LQQKGFAP 1078



 Score = 62.0 bits (149), Expect = 5e-06
 Identities = 32/68 (47%), Positives = 40/68 (58%)
 Frame = +2

Query: 2   VCNAGVVENLIDWVKYXXXXXXXXXXXXXXXXITGISKLEDANEAGCTRAKDCTLILTEG 181
           V  +GVV +L+ W  +                I GI KLEDAN+AG   ++ CTLILTEG
Sbjct: 404 VAKSGVVNSLLSWADFKQSKELKKTDGTKRQRIVGIPKLEDANDAGGRNSEKCTLILTEG 463

Query: 182 DSAKALAV 205
           DSAKALA+
Sbjct: 464 DSAKALAM 471


>ref|XP_006345427.1| PREDICTED: DNA topoisomerase 2 isoform X3 [Solanum tuberosum]
          Length = 1485

 Score =  899 bits (2324), Expect = 0.0
 Identities = 426/607 (70%), Positives = 524/607 (86%), Gaps = 1/607 (0%)
 Frame = +1

Query: 208  MAGISAVGRDCYGVFPLRGKLLNVREANNYQISGNEEIQSIMKILRLEYGKNYNSVESLR 387
            MAGIS VGRD YGVFPLRGKLLNVREA++ Q+S N+EI+SI KIL L+ GK Y SV+SLR
Sbjct: 462  MAGISVVGRDHYGVFPLRGKLLNVREASHKQVSENKEIESIKKILGLQTGKEYESVKSLR 521

Query: 388  YGHVMIMTDQDHDGSHIKGLLINLFHSLWPSLLKIPTFLLEFITPIIKAT-RAGQVLSFY 564
            YGH+MIMTDQDHDGSHIKGLLIN  H+ WPSLLK+P+FL+EFITPI+KAT ++G++L+FY
Sbjct: 522  YGHLMIMTDQDHDGSHIKGLLINFIHTFWPSLLKVPSFLVEFITPIVKATHKSGRILAFY 581

Query: 565  SIPEYEAWKQSLDGNNSGWSIKYYKGLGTSTSKEGKEYFQDLEKHKKDFLWLDKKDNDAI 744
            ++PEYEAW++SL   +SGWSIKYYKGLGTSTSKEGKEYFQDL+KH+KDF+W D +D ++I
Sbjct: 582  TMPEYEAWRRSLGATSSGWSIKYYKGLGTSTSKEGKEYFQDLQKHRKDFIWADNQDGESI 641

Query: 745  ELAFSKHKIQARKKWLQKYEPGTYLDQKEKHIKYSDFVDKELIQFSVADLKRSIPSMVDG 924
            ELAFSK KI+ARK WL+++EPGT+LDQKEK+I Y++FV+KELI FS+ADL+RSIPSM+DG
Sbjct: 642  ELAFSKKKIEARKNWLRQFEPGTHLDQKEKYISYTEFVNKELILFSMADLQRSIPSMLDG 701

Query: 925  FKPGQRKILFSCFKRNFIKEAKVAQLSGYVSEHSAYHHGEQSLANTIVGMAQDYMGSNNI 1104
             KPGQRKILF  FKRNF+KEAKV+Q SGYVSEHSAYHHGEQSL++TI+GMAQDY+GSNN+
Sbjct: 702  LKPGQRKILFCAFKRNFVKEAKVSQFSGYVSEHSAYHHGEQSLSSTIIGMAQDYVGSNNV 761

Query: 1105 NLLQPNGQFGTRHQGGKDHASARYIYTCLSPITRFLFNKNDDALLHYLNEDGQSVEPTWY 1284
            NLLQPNGQFGTR+ GGKDHAS+RYIYT LSPI RFLF K+DD +L YLNEDGQS+EPTWY
Sbjct: 762  NLLQPNGQFGTRNMGGKDHASSRYIYTRLSPIARFLFPKDDDTVLDYLNEDGQSIEPTWY 821

Query: 1285 VPVIPMVLVNGTEGIGTGWSTYIPNYNPRDIVANVRHFLNDEPMEPMIPWYKGFKGTIES 1464
            +P+IPMVLVNG+EGIGTGWS+Y+PNYNPRD+VANVR  LNDE MEPM PWYKGFKGTIE 
Sbjct: 822  IPIIPMVLVNGSEGIGTGWSSYVPNYNPRDLVANVRRLLNDELMEPMDPWYKGFKGTIEK 881

Query: 1465 TNTKDAGKSYVVKGIIEEVNKTTVRITELPVRKSTEEYKQFLDKLISKGGRNKEQFIKDF 1644
            T TK+ G +Y V GIIEE ++TT+RI+ELPVR+ TE+YKQFL+ +     ++K+ FIK+ 
Sbjct: 882  TATKETGATYTVTGIIEEFSETTLRISELPVRRWTEDYKQFLESMTVSTDKSKDAFIKEV 941

Query: 1645 REFNDDKTVHFEIYMSEKNLAMAKEEGLIEKFKLSTTISTSNMHLFNPKGVLAKYSSPEK 1824
            R + D+ +V FE+ MSE+NL +A++EGL++KFKL+TTISTSNMHLF+ KG + KY +PE+
Sbjct: 942  RAYGDENSVCFEVIMSEENLLLAQQEGLLKKFKLTTTISTSNMHLFDSKGKIKKYDAPEE 1001

Query: 1825 IIEEFCDLRLEFYEKRKIFMLSMXXXXXXXXXNKVRFIDEVIQGKIILMNRKRSDLLDEL 2004
            I+EEF  +RLE+YEKRK  +L +         NKVRFI  V++G+II+ NRKR+DLL EL
Sbjct: 1002 ILEEFYHVRLEYYEKRKKTLLEILELELLRIENKVRFILGVVKGEIIVNNRKRADLLLEL 1061

Query: 2005 KQKNFTP 2025
            K K FTP
Sbjct: 1062 KNKGFTP 1068