BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00013687
         (2684 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branchin...  1344   0.0  
ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum...  1333   0.0  
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...  1249   0.0  
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea...  1220   0.0  
ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea...  1218   0.0  
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]       1191   0.0  
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...  1182   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                            1105   0.0  
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1084   0.0  
ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [...  1083   0.0  
ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1082   0.0  
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] >gi...  1081   0.0  
ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Sola...  1080   0.0  
ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1078   0.0  
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1078   0.0  
ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1076   0.0  
gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]       1074   0.0  
ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Sola...  1074   0.0  
gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]         1071   0.0  
ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1071   0.0  

>gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
            [Handroanthus impetiginosus]
          Length = 853

 Score = 1344 bits (3478), Expect = 0.0
 Identities = 661/825 (80%), Positives = 727/825 (88%), Gaps = 1/825 (0%)
 Frame = -1

Query: 2675 RRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMME 2496
            +RVTS+S KL+LKQ+ V GQLMKP RRIS +HHVLKALATSDVSVV+ SENVATYRF ME
Sbjct: 33   KRVTSNSQKLDLKQRRVFGQLMKPFRRISCNHHVLKALATSDVSVVETSENVATYRFRME 92

Query: 2495 IGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSAD 2316
             GGQLKV +KKK+NNK+ L+VEVE L+   GQ ELF++WGLFR  +T FMPL F++SSAD
Sbjct: 93   TGGQLKVLVKKKNNNKHVLYVEVESLQLLGGQRELFLVWGLFRDHATTFMPLGFQKSSAD 152

Query: 2315 SKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIV 2136
               +TIETPF K+S    AVE+DFDE+L PFYVS LLKSQ GS+LK+STIRSHRKTNFIV
Sbjct: 153  D--NTIETPFMKDSSDRFAVEVDFDENLPPFYVSFLLKSQCGSDLKISTIRSHRKTNFIV 210

Query: 2135 PVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNRS 1956
            PVGFGSGRP PLGISF DDG  NFAFFS+N ESVVLCLY+DT  D+PALEIDLDPYVNR+
Sbjct: 211  PVGFGSGRPFPLGISFLDDGSTNFAFFSQNAESVVLCLYSDTTVDEPALEIDLDPYVNRT 270

Query: 1955 GDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEP 1776
            GDIWHA VDCS  FV YGYRCR+G +NKGH VLLDPYA+VIED GLDLP+++LGKL +EP
Sbjct: 271  GDIWHASVDCSPHFVSYGYRCRSGPDNKGHNVLLDPYAKVIEDIGLDLPQRYLGKLFKEP 330

Query: 1775 AFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNA 1596
             FDWSG+F PS+PME+LIIYRLNVTRFT   SSKLPGDIAG+F+G+SEKLHHFK+LGVNA
Sbjct: 331  PFDWSGEFCPSIPMEKLIIYRLNVTRFTRHNSSKLPGDIAGSFSGVSEKLHHFKDLGVNA 390

Query: 1595 VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVV 1416
            VLLEPIFPFDE+K PYFP HFFSPG+LYGPSG+P +VAKSMKEMVKKLHASGIEVLLEVV
Sbjct: 391  VLLEPIFPFDEKKRPYFPAHFFSPGNLYGPSGNPNSVAKSMKEMVKKLHASGIEVLLEVV 450

Query: 1415 FTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGF 1236
            FTHT E  SLREIDNS YY + GGEDLK  NALNCNYPIVQQMILESLRYWV EFHIDGF
Sbjct: 451  FTHTGETGSLREIDNSCYYHIKGGEDLKLGNALNCNYPIVQQMILESLRYWVTEFHIDGF 510

Query: 1235 CFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRW 1056
            CF+NASSLTRG HGEFLSRPPL+EAIAFDPLLSKVK+IADSWDPH +ETKE AFPHWKRW
Sbjct: 511  CFINASSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIIADSWDPHDMETKEIAFPHWKRW 570

Query: 1055 AEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLV 876
            AEMN +FCVDVRNFLRG+GPI+NLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLV
Sbjct: 571  AEMNARFCVDVRNFLRGKGPINNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLV 630

Query: 875  SFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQST 696
            SFS SDLE + SWNCGEEGPTNK+VVLE RLKQIRNFLF+LFISLGVPVLNMGDECGQS+
Sbjct: 631  SFSNSDLESELSWNCGEEGPTNKSVVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSS 690

Query: 695  GGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTE 516
            GGS  Y DRK LDWN LKSGFGIQVTQFISFLSSL++RRSDLLQ RSFLKE+NIEW GT+
Sbjct: 691  GGSTAYADRKLLDWNALKSGFGIQVTQFISFLSSLRIRRSDLLQKRSFLKEDNIEWHGTQ 750

Query: 515  QFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLA-VD 339
            Q PP WDD SCKFL+MTLK +A P  S +     GGDLFIAFNS DHS KV +PPLA VD
Sbjct: 751  QSPPTWDDPSCKFLAMTLKAEAPPLSSPN---VIGGDLFIAFNSADHSAKVAVPPLASVD 807

Query: 338  ATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARS 204
            ATWV LVDTALP PGFF+ DGVPL+AGL TYEMKSHSCILFEARS
Sbjct: 808  ATWVRLVDTALPLPGFFSKDGVPLDAGLVTYEMKSHSCILFEARS 852


>ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum]
 ref|XP_011092270.1| isoamylase 2, chloroplastic [Sesamum indicum]
          Length = 857

 Score = 1333 bits (3450), Expect = 0.0
 Identities = 651/827 (78%), Positives = 722/827 (87%), Gaps = 1/827 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNL-KQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            G+R+ S   +L++ KQ   +G+ +K  RRISY  HVLK++A SDVSVVK SEN+ATYRF 
Sbjct: 32   GKRLVSGPKRLDVAKQAMFNGRQVKYVRRISYGPHVLKSVANSDVSVVKASENMATYRFW 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
            MEIGGQL+  +K+KSNNKYGL+VEVE  +  EG  EL MIWGLFR+DST FMPLDF++SS
Sbjct: 92   MEIGGQLRALVKRKSNNKYGLYVEVESSQLYEGHSELVMIWGLFRADSTNFMPLDFQRSS 151

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            ADSK STIETPF K+S G LAVELDF+ESLAPFYVSILLKS++GSN K+STIRS RKTNF
Sbjct: 152  ADSKYSTIETPFVKDSNGRLAVELDFEESLAPFYVSILLKSKIGSNSKISTIRSLRKTNF 211

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            IVPVGFGSG PSPLGISF+D+G INFAFFSR+ E VVLCLY D   +KPALEIDLDPYVN
Sbjct: 212  IVPVGFGSGYPSPLGISFSDNGFINFAFFSRDAERVVLCLYMDNTLEKPALEIDLDPYVN 271

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCE 1782
            RSGDIWHA +D S+PFV YGYRCR+ T+NKGH VLLDPYA+VIED GL LPRK LGKL +
Sbjct: 272  RSGDIWHASIDSSMPFVSYGYRCRSATDNKGHHVLLDPYAKVIEDLGLGLPRKCLGKLYK 331

Query: 1781 EPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGV 1602
            EPA+DWSG+F PSLPME+LIIYRLN+T FT +KSSKLPGDIAGTF+G+SEKLHHFK+LGV
Sbjct: 332  EPAYDWSGEFHPSLPMEKLIIYRLNITGFTKNKSSKLPGDIAGTFSGLSEKLHHFKDLGV 391

Query: 1601 NAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLE 1422
            NA+LLEPIFPFDE+KGPYFPWHFFSP  L GP+GDP TVA+SMKEMVKKLH SGIEVLLE
Sbjct: 392  NAILLEPIFPFDEKKGPYFPWHFFSPERLCGPTGDPTTVAQSMKEMVKKLHGSGIEVLLE 451

Query: 1421 VVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHID 1242
            VVFTHT E  +LR+ID SSYY   GG+DL SRNALNCNYPIVQQM+LESLRYWVIEFHID
Sbjct: 452  VVFTHTGEGCALRDID-SSYYHTEGGDDLTSRNALNCNYPIVQQMVLESLRYWVIEFHID 510

Query: 1241 GFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWK 1062
            GFCF+NAS L RG HGEFLSRPPL+EAIAFDP+LSK K+IADSWDPH +ETKE  FPHWK
Sbjct: 511  GFCFINASHLMRGFHGEFLSRPPLVEAIAFDPVLSKAKIIADSWDPHEMETKEIVFPHWK 570

Query: 1061 RWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVD 882
            RWAEMN KFCVDVRNFLRGQGPISNLATRLCGSGDLFL GRGPAFS NFITRNVGLTLVD
Sbjct: 571  RWAEMNAKFCVDVRNFLRGQGPISNLATRLCGSGDLFLGGRGPAFSINFITRNVGLTLVD 630

Query: 881  LVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQ 702
            LVSFS S    + SWNCGEEGPTNKT+VLE RLKQIRNFLF+LFISLGVPVLNMGDECGQ
Sbjct: 631  LVSFSSSTFGSELSWNCGEEGPTNKTLVLERRLKQIRNFLFILFISLGVPVLNMGDECGQ 690

Query: 701  STGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRG 522
            STGGS  Y DR+PLDWN L+SGFG QVTQFISFLS L+ RRSD+LQ RSFLKE+NIEW G
Sbjct: 691  STGGSQEYADRRPLDWNALRSGFGTQVTQFISFLSLLRRRRSDILQRRSFLKEDNIEWHG 750

Query: 521  TEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAV 342
            T++ PP WDD SCKFL+MTLK DA PSQ TSGS NAGGDLF+AFNS +H +KV LP LAV
Sbjct: 751  TDRTPPMWDDPSCKFLAMTLKADALPSQVTSGSPNAGGDLFVAFNSANHLQKVALPQLAV 810

Query: 341  DATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
             +TWV LVDTALPFPGFFTVDGVPL+AGLATYEMKSHSCILFEAR L
Sbjct: 811  GSTWVRLVDTALPFPGFFTVDGVPLDAGLATYEMKSHSCILFEARKL 857


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842326.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842327.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842328.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
          Length = 840

 Score = 1249 bits (3232), Expect = 0.0
 Identities = 624/829 (75%), Positives = 698/829 (84%), Gaps = 4/829 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNLKQKTVHG-QLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            G+RVTSDS KL+ ++  +HG Q  KP  RIS  HHV KA+ATSDVS VK+ ENV TYRF 
Sbjct: 34   GKRVTSDSRKLDFRRINLHGGQFAKPIGRISCSHHVTKAIATSDVSAVKILENVLTYRFW 93

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
            MEIGGQLKV +KK +NNKYG++VEVE L+  E Q EL MIWG+FR D T FMP+  +QSS
Sbjct: 94   MEIGGQLKVMVKKNNNNKYGVYVEVESLQLDEEQRELIMIWGIFRDDLTKFMPV--QQSS 151

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
             D    T ETPF K+    L+VEL+F+ESLAPFYVSILLKS+LGSN K STIRSHRKTNF
Sbjct: 152  TDG---TTETPFVKDC-DRLSVELEFEESLAPFYVSILLKSKLGSNSKTSTIRSHRKTNF 207

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            IVPVGF SGRPSPLGIS  +DG INFAFFSRN  SVVLCLYADT  DKPALEIDLDPYVN
Sbjct: 208  IVPVGFRSGRPSPLGISLLNDGFINFAFFSRNAYSVVLCLYADTTVDKPALEIDLDPYVN 267

Query: 1961 RSGDIWHALVDCS-LPFVGYGYRCRNG-TENKGHRVLLDPYARVIEDFGLDLPRKFLGKL 1788
            RSGDIWHA +DCS LPFV YGYRCR+  T++KGHRVL DPYA+VIE+FG   PRK LG+L
Sbjct: 268  RSGDIWHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRL 324

Query: 1787 CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 1608
            C E AFDWSG+ RP+LPME+LI+YRLNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNL
Sbjct: 325  CNESAFDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNL 384

Query: 1607 GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 1428
            GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVL
Sbjct: 385  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVL 444

Query: 1427 LEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 1248
            LEVVFTHTSE+++LREIDNSSY+ V GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFH
Sbjct: 445  LEVVFTHTSEISALREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFH 504

Query: 1247 IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPH 1068
            IDGFCF+N+SSLTRG HGEFLSRPPL+EAIAFDPLLSKVK++ADSWDPH +E KE  FPH
Sbjct: 505  IDGFCFINSSSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPH 564

Query: 1067 WKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTL 888
            WKRWAEMN KFC ++RNF RG+G IS+LATRLCGSGDLFL GR PAFSFNF+TRNVG TL
Sbjct: 565  WKRWAEMNAKFCSEMRNFFRGKGSISSLATRLCGSGDLFLGGRSPAFSFNFVTRNVGPTL 624

Query: 887  VDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDEC 708
            VDLVS++      + SWNCGEEGPTNK +VLETRLKQIRNF+FVLF+SLGVPVLNMGDEC
Sbjct: 625  VDLVSYNSI----ESSWNCGEEGPTNKILVLETRLKQIRNFIFVLFVSLGVPVLNMGDEC 680

Query: 707  GQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEW 528
            GQST GSP Y+DR   DW+ LKSGFG QVT+FISFLSSL++RR+DLLQ RSFLKE+NIEW
Sbjct: 681  GQSTRGSPEYSDRISFDWSALKSGFGTQVTEFISFLSSLRIRRNDLLQKRSFLKEDNIEW 740

Query: 527  RGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPL 348
             G E  PP WDD SCKFL+MTLK D   S           DLF+AFN GD SEKV LPPL
Sbjct: 741  HGAEGGPPMWDDPSCKFLAMTLKADPPLS-----------DLFVAFNGGDCSEKVALPPL 789

Query: 347  -AVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARS 204
             AVD  WVCLVDT+LPFPGFFT +GVPLE GL TYEMKSHSC+LFEARS
Sbjct: 790  AAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLETYEMKSHSCVLFEARS 838


>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
            sylvestris]
          Length = 849

 Score = 1220 bits (3157), Expect = 0.0
 Identities = 599/823 (72%), Positives = 689/823 (83%)
 Frame = -1

Query: 2669 VTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMMEIG 2490
            VT    K++ K K V   L K  RRIS++ H  KALATSDVSV + +E VA YRF  E G
Sbjct: 31   VTCGFQKMDAKYKQVLSGLGKSVRRISHELHTWKALATSDVSV-QTAEKVAIYRFRTENG 89

Query: 2489 GQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSK 2310
            GQLK+ ++K +N KYG++VEV    H   +G+L M WGLFRS+S  FMP+DF+ SSADSK
Sbjct: 90   GQLKLLVRK-NNKKYGVYVEVLSPLHSGRKGDLLMSWGLFRSNSESFMPMDFENSSADSK 148

Query: 2309 QSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPV 2130
             STI+TPF K+S G L VELDFDESLAPFY+S+LLKS LGS+L+ S IRSHRKTNFIVPV
Sbjct: 149  YSTIDTPFVKDSSGRLVVELDFDESLAPFYISVLLKS-LGSDLESSEIRSHRKTNFIVPV 207

Query: 2129 GFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGD 1950
            GF SGRP+PLG+S   +G +NFAFFSRN ESVVLCLYADT A+KPALEIDLDPYVNRSGD
Sbjct: 208  GFRSGRPTPLGLSILANGSMNFAFFSRNAESVVLCLYADTTANKPALEIDLDPYVNRSGD 267

Query: 1949 IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 1770
            IWH L+D S+PFV YGYRC++  + K   VLLDPYA++IEDFG  LPRK LGKL +E  F
Sbjct: 268  IWHVLLDGSVPFVRYGYRCKSNADKKRQYVLLDPYAKLIEDFGSGLPRKSLGKLWKESGF 327

Query: 1769 DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 1590
            DWSGD RPSLPME+LIIYRLNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNA+L
Sbjct: 328  DWSGDMRPSLPMEKLIIYRLNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAIL 387

Query: 1589 LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 1410
            LEPIFPFDEQ GPYFPWHFFSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFT
Sbjct: 388  LEPIFPFDEQTGPYFPWHFFSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFT 447

Query: 1409 HTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 1230
            HT+E+A+L++ID S YY V GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF
Sbjct: 448  HTAEVAALKQIDRS-YYHVGGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCF 506

Query: 1229 VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAE 1050
            ++ASSL +G HGEFL+RPPL+EAIAFDPLLSKVK+IADSWDPH++ETK+ +FPHWKRWAE
Sbjct: 507  IDASSLMKGFHGEFLTRPPLVEAIAFDPLLSKVKIIADSWDPHNMETKDISFPHWKRWAE 566

Query: 1049 MNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSF 870
            +N KFC DVRNFLRGQG I NLATRLCGSG+LFL GRGPAFSFNFI+RN GL LVDLV F
Sbjct: 567  INSKFCNDVRNFLRGQGSIGNLATRLCGSGNLFLDGRGPAFSFNFISRNAGLPLVDLVCF 626

Query: 869  SGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGG 690
            S S+L  + SWNCGEEGPTNK  VLE RLKQIRN+LF+LF SLGVPVLNMGDECGQS+GG
Sbjct: 627  SSSELASELSWNCGEEGPTNKNAVLERRLKQIRNYLFILFFSLGVPVLNMGDECGQSSGG 686

Query: 689  SPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQF 510
            SP + DRKPLDWN LKSGF IQ T+FI+FLSSLK+RRSDLLQNR+F KEENIEW G++  
Sbjct: 687  SPAHADRKPLDWNALKSGFAIQTTKFITFLSSLKMRRSDLLQNRNFWKEENIEWHGSDGS 746

Query: 509  PPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATW 330
            PP+WD+ SC+FL+MTLK D++ SQ TSG     GDLFIAFNS   SE + LPPL  D  W
Sbjct: 747  PPRWDEPSCRFLAMTLKADSKESQPTSGLHEMIGDLFIAFNSAGRSENIVLPPLTEDIAW 806

Query: 329  VCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
              LVDT+LPFPGFFT DGVPL+ G  TYEMKSHSC+LFEARSL
Sbjct: 807  FRLVDTSLPFPGFFTTDGVPLKDGSVTYEMKSHSCVLFEARSL 849


>ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea europaea var.
            sylvestris]
          Length = 829

 Score = 1218 bits (3152), Expect = 0.0
 Identities = 597/817 (73%), Positives = 687/817 (84%)
 Frame = -1

Query: 2651 KLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMMEIGGQLKVF 2472
            K++ K K V   L K  RRIS++ H  KALATSDVSV + +E VA YRF  E GGQLK+ 
Sbjct: 17   KMDAKYKQVLSGLGKSVRRISHELHTWKALATSDVSV-QTAEKVAIYRFRTENGGQLKLL 75

Query: 2471 IKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSKQSTIET 2292
            ++K +N KYG++VEV    H   +G+L M WGLFRS+S  FMP+DF+ SSADSK STI+T
Sbjct: 76   VRK-NNKKYGVYVEVLSPLHSGRKGDLLMSWGLFRSNSESFMPMDFENSSADSKYSTIDT 134

Query: 2291 PFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPVGFGSGR 2112
            PF K+S G L VELDFDESLAPFY+S+LLKS LGS+L+ S IRSHRKTNFIVPVGF SGR
Sbjct: 135  PFVKDSSGRLVVELDFDESLAPFYISVLLKS-LGSDLESSEIRSHRKTNFIVPVGFRSGR 193

Query: 2111 PSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGDIWHALV 1932
            P+PLG+S   +G +NFAFFSRN ESVVLCLYADT A+KPALEIDLDPYVNRSGDIWH L+
Sbjct: 194  PTPLGLSILANGSMNFAFFSRNAESVVLCLYADTTANKPALEIDLDPYVNRSGDIWHVLL 253

Query: 1931 DCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAFDWSGDF 1752
            D S+PFV YGYRC++  + K   VLLDPYA++IEDFG  LPRK LGKL +E  FDWSGD 
Sbjct: 254  DGSVPFVRYGYRCKSNADKKRQYVLLDPYAKLIEDFGSGLPRKSLGKLWKESGFDWSGDM 313

Query: 1751 RPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVLLEPIFP 1572
            RPSLPME+LIIYRLNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNA+LLEPIFP
Sbjct: 314  RPSLPMEKLIIYRLNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAILLEPIFP 373

Query: 1571 FDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELA 1392
            FDEQ GPYFPWHFFSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFTHT+E+A
Sbjct: 374  FDEQTGPYFPWHFFSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFTHTAEVA 433

Query: 1391 SLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSL 1212
            +L++ID S YY V GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF++ASSL
Sbjct: 434  ALKQIDRS-YYHVGGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCFIDASSL 492

Query: 1211 TRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAEMNGKFC 1032
             +G HGEFL+RPPL+EAIAFDPLLSKVK+IADSWDPH++ETK+ +FPHWKRWAE+N KFC
Sbjct: 493  MKGFHGEFLTRPPLVEAIAFDPLLSKVKIIADSWDPHNMETKDISFPHWKRWAEINSKFC 552

Query: 1031 VDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSFSGSDLE 852
             DVRNFLRGQG I NLATRLCGSG+LFL GRGPAFSFNFI+RN GL LVDLV FS S+L 
Sbjct: 553  NDVRNFLRGQGSIGNLATRLCGSGNLFLDGRGPAFSFNFISRNAGLPLVDLVCFSSSELA 612

Query: 851  PQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGGSPVYTD 672
             + SWNCGEEGPTNK  VLE RLKQIRN+LF+LF SLGVPVLNMGDECGQS+GGSP + D
Sbjct: 613  SELSWNCGEEGPTNKNAVLERRLKQIRNYLFILFFSLGVPVLNMGDECGQSSGGSPAHAD 672

Query: 671  RKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQFPPKWDD 492
            RKPLDWN LKSGF IQ T+FI+FLSSLK+RRSDLLQNR+F KEENIEW G++  PP+WD+
Sbjct: 673  RKPLDWNALKSGFAIQTTKFITFLSSLKMRRSDLLQNRNFWKEENIEWHGSDGSPPRWDE 732

Query: 491  ESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATWVCLVDT 312
             SC+FL+MTLK D++ SQ TSG     GDLFIAFNS   SE + LPPL  D  W  LVDT
Sbjct: 733  PSCRFLAMTLKADSKESQPTSGLHEMIGDLFIAFNSAGRSENIVLPPLTEDIAWFRLVDT 792

Query: 311  ALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            +LPFPGFFT DGVPL+ G  TYEMKSHSC+LFEARSL
Sbjct: 793  SLPFPGFFTTDGVPLKDGSVTYEMKSHSCVLFEARSL 829


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score = 1191 bits (3082), Expect = 0.0
 Identities = 590/823 (71%), Positives = 669/823 (81%)
 Frame = -1

Query: 2669 VTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMMEIG 2490
            VT D  K +LKQK    +L K   RIS +HHV+KALATSDVSVV+ +  +A+YRF  EIG
Sbjct: 31   VTRDLVKFDLKQKKDRVRLNKFGGRISLEHHVMKALATSDVSVVQTTGKLASYRFRTEIG 90

Query: 2489 GQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSADSK 2310
            GQLKV ++K  N+K+ +HVE   L   E +G L M WGLFRSDS+ FMPL+F+QS+ADSK
Sbjct: 91   GQLKVLVRK--NSKFEVHVEASNLPGEEDEG-LIMRWGLFRSDSSIFMPLNFQQSTADSK 147

Query: 2309 QSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFIVPV 2130
             + +ETPF K+SVG LAVELDFD SL PFYVS LLKS   SN K+ TIRSHR TNF+VPV
Sbjct: 148  DNVVETPFLKDSVGRLAVELDFDASLTPFYVSFLLKS---SNSKIPTIRSHRTTNFVVPV 204

Query: 2129 GFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNRSGD 1950
            GFGSGRP PLG+SF DD  INFAFFS N E VVLCLY+D  ADKPALEIDLDPYVNRSG 
Sbjct: 205  GFGSGRPFPLGLSFLDDRSINFAFFSSNAEGVVLCLYSDGTADKPALEIDLDPYVNRSGY 264

Query: 1949 IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 1770
            IWHAL+  SLPFV YGYRCR G  N+G  VLLDPYA VI+ FGL L RK+LGKLCEEPAF
Sbjct: 265  IWHALIQDSLPFVSYGYRCRGGIANRGQHVLLDPYATVIKSFGLGLNRKWLGKLCEEPAF 324

Query: 1769 DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 1590
            DWSG+ RPSLP+E+LI+YRLNVTRFT DKSSKLP   AGTF GI EKLHHFK+LGVNA+L
Sbjct: 325  DWSGESRPSLPLEKLIVYRLNVTRFTKDKSSKLPTSAAGTFYGIYEKLHHFKDLGVNAIL 384

Query: 1589 LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 1410
            LEPIFP DE+K PYFPWHFFSPGS YGPS D   + KSMKEMVKKLHASGIEVLLEVVFT
Sbjct: 385  LEPIFPSDERKEPYFPWHFFSPGSSYGPSDDLSYIVKSMKEMVKKLHASGIEVLLEVVFT 444

Query: 1409 HTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 1230
            HT E  +L+ ID S Y+S+   EDLKS+N LNCNYP+VQQ+ILESLR+WVIE+HIDGFCF
Sbjct: 445  HTGEDVTLKGIDESYYHSI---EDLKSKNMLNCNYPVVQQLILESLRHWVIEYHIDGFCF 501

Query: 1229 VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPHWKRWAE 1050
            +NASSL RG HGEFLSRPPL+EAIAFDPLLS++K+IADSWDPH +E+KE  FPHWKRWAE
Sbjct: 502  INASSLLRGKHGEFLSRPPLVEAIAFDPLLSQIKIIADSWDPHGMESKELVFPHWKRWAE 561

Query: 1049 MNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTLVDLVSF 870
            MNGKFC D RNFLRGQG IS+L TR CGSGD+F  GRGPAFSFN+ITRNVGLTL+DLVSF
Sbjct: 562  MNGKFCADARNFLRGQGSISSLTTRFCGSGDIFAGGRGPAFSFNYITRNVGLTLIDLVSF 621

Query: 869  SGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDECGQSTGG 690
               ++  Q SWNCGEEGPTNK  VLE RLKQIRNFLF+LF+SLGVPVLNMGDE GQS  G
Sbjct: 622  GSEEIASQLSWNCGEEGPTNKISVLERRLKQIRNFLFLLFLSLGVPVLNMGDEFGQSCAG 681

Query: 689  SPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEWRGTEQF 510
            S  YTDR PLDWN L+SGF IQ+TQFISFL SLK+RR D  Q +SFL+E+ IEW GT+  
Sbjct: 682  SLAYTDRTPLDWNSLRSGFSIQITQFISFLCSLKMRRGDFFQRKSFLEEDKIEWHGTDLS 741

Query: 509  PPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPLAVDATW 330
            PPKWDD S KF+++TLK   + SQSTS      GDLF+AFNS D SE+V LPPL  +  W
Sbjct: 742  PPKWDDPSTKFVAVTLKVGNKLSQSTSAFPEPSGDLFVAFNSDDQSEEVALPPLPDNTAW 801

Query: 329  VCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            V LVDTAL FPGFF+VDGVPLE GL TYEM+SHSC+LFEAR+L
Sbjct: 802  VRLVDTALTFPGFFSVDGVPLEDGLTTYEMQSHSCVLFEARNL 844


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score = 1182 bits (3058), Expect = 0.0
 Identities = 587/769 (76%), Positives = 654/769 (85%), Gaps = 3/769 (0%)
 Frame = -1

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
            MEIGGQLKV +KK +NNKYG++VEVE L+  E Q EL MIWG+FR D T FMP+  +QSS
Sbjct: 1    MEIGGQLKVMVKKNNNNKYGVYVEVESLQLDEEQRELIMIWGIFRDDLTKFMPV--QQSS 58

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
             D    T ETPF K+    L+VEL+F+ESLAPFYVSILLKS+LGSN K STIRSHRKTNF
Sbjct: 59   TDG---TTETPFVKDC-DRLSVELEFEESLAPFYVSILLKSKLGSNSKTSTIRSHRKTNF 114

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            IVPVGF SGRPSPLGIS  +DG INFAFFSRN  SVVLCLYADT  DKPALEIDLDPYVN
Sbjct: 115  IVPVGFRSGRPSPLGISLLNDGFINFAFFSRNAYSVVLCLYADTTVDKPALEIDLDPYVN 174

Query: 1961 RSGDIWHALVDCS-LPFVGYGYRCRNG-TENKGHRVLLDPYARVIEDFGLDLPRKFLGKL 1788
            RSGDIWHA +DCS LPFV YGYRCR+  T++KGHRVL DPYA+VIE+FG   PRK LG+L
Sbjct: 175  RSGDIWHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRL 231

Query: 1787 CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 1608
            C E AFDWSG+ RP+LPME+LI+YRLNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNL
Sbjct: 232  CNESAFDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNL 291

Query: 1607 GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 1428
            GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVL
Sbjct: 292  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVL 351

Query: 1427 LEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 1248
            LEVVFTHTSE+++LREIDNSSY+ V GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFH
Sbjct: 352  LEVVFTHTSEISALREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFH 411

Query: 1247 IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAFPH 1068
            IDGFCF+N+SSLTRG HGEFLSRPPL+EAIAFDPLLSKVK++ADSWDPH +E KE  FPH
Sbjct: 412  IDGFCFINSSSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPH 471

Query: 1067 WKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGLTL 888
            WKRWAEMN KFC ++RNF RG+G IS+LATRLCGSGDLFL GR PAFSFNF+TRNVG TL
Sbjct: 472  WKRWAEMNAKFCSEMRNFFRGKGSISSLATRLCGSGDLFLGGRSPAFSFNFVTRNVGPTL 531

Query: 887  VDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGDEC 708
            VDLVS++      + SWNCGEEGPTNK +VLETRLKQIRNF+FVLF+SLGVPVLNMGDEC
Sbjct: 532  VDLVSYNSI----ESSWNCGEEGPTNKILVLETRLKQIRNFIFVLFVSLGVPVLNMGDEC 587

Query: 707  GQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENIEW 528
            GQST GSP Y+DR   DW+ LKSGFG QVT+FISFLSSL++RR+DLLQ RSFLKE+NIEW
Sbjct: 588  GQSTRGSPEYSDRISFDWSALKSGFGTQVTEFISFLSSLRIRRNDLLQKRSFLKEDNIEW 647

Query: 527  RGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLPPL 348
             G E  PP WDD SCKFL+MTLK D   S           DLF+AFN GD SEKV LPPL
Sbjct: 648  HGAEGGPPMWDDPSCKFLAMTLKADPPLS-----------DLFVAFNGGDCSEKVALPPL 696

Query: 347  -AVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARS 204
             AVD  WVCLVDT+LPFPGFFT +GVPLE GL TYEMKSHSC+LFEARS
Sbjct: 697  AAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLETYEMKSHSCVLFEARS 745


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score = 1105 bits (2858), Expect = 0.0
 Identities = 537/835 (64%), Positives = 650/835 (77%), Gaps = 8/835 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N R+  + + + + K+  VHG++ K A + S+ H  LK LATS +SV +  E  + Y F 
Sbjct: 32   NYRKRVAQNLQQSYKRNFVHGEVKKYATKDSFQHRNLKVLATSRISVGQKEEKSSAYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             EIGG +KV +++K N KY ++VEV  L+   G+ +L M WG+FRSDS+ F+PLD + S+
Sbjct: 92   TEIGGHVKVLVRQK-NLKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDSSQFIPLDLQSST 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
             D + ST+ETPF +NS+G L +EL+F+ SL PFY+S LLK     + K S +RSHRKT+F
Sbjct: 151  PDDRSSTVETPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
             VPVG  SG P+PLG+SF+ DG INFA FS++ + VVLCLY DT+A+KP LEIDLDPYVN
Sbjct: 211  CVPVGLASGYPAPLGLSFSADGSINFALFSQSADGVVLCLYDDTKAEKPTLEIDLDPYVN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKG-----HRVLLDPYARVIEDFGLDLPRKFL 1797
            RSGDIWH  +D +LPFV YGYRC+ G   KG       VLLDPYA+VI +       K L
Sbjct: 271  RSGDIWHVSMDSALPFVSYGYRCKGGIHGKGVKDHSEHVLLDPYAKVIANAHSGSVLKTL 330

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            GKL +EP FDWSGD RP +PME+L++YR+NV  FT  KSS LP D+AG+F GI+EKL H 
Sbjct: 331  GKLIKEPTFDWSGDIRPCIPMEKLVVYRVNVAHFTKHKSSGLPNDVAGSFTGITEKLQHC 390

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEP+FPFDE+KGPYFP+HFFSP  LYGPS DP     SMKEMVK LHA+GI
Sbjct: 391  KDLGVNAILLEPVFPFDEKKGPYFPFHFFSPMKLYGPSDDPSATVNSMKEMVKILHANGI 450

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            EVLLE+VFTHT+E  SLREID SSYY + G EDL ++NALNCNYPIVQQMIL+SLRYW I
Sbjct: 451  EVLLEIVFTHTAESCSLREIDKSSYY-ITGDEDLGAKNALNCNYPIVQQMILDSLRYWAI 509

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFH+DGFCF+NASSL +G HGEFLSRPPL+EAIAFDPLLSK KLIADSWDPH + + E  
Sbjct: 510  EFHVDGFCFINASSLLKGFHGEFLSRPPLVEAIAFDPLLSKTKLIADSWDPHDMASDEIL 569

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHWKRWAE+N  FC DVRNFLRG+G +SNLATRLCGSGD+FL+GRGP FSFN+ITRN G
Sbjct: 570  FPHWKRWAEVNTNFCGDVRNFLRGEGLLSNLATRLCGSGDMFLNGRGPGFSFNYITRNSG 629

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            L LVDLVSFS S+L  + SWNCGEEGPTN T VLE RLKQIRN+LF+LF+S GVPVLNMG
Sbjct: 630  LPLVDLVSFSSSNLASELSWNCGEEGPTNNTAVLERRLKQIRNYLFILFVSFGVPVLNMG 689

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GGSP Y DRKP DWN L++GFGIQ TQFISFLSSL+VRRSDLLQ RSFLKEEN
Sbjct: 690  DECGQSSGGSPAYGDRKPYDWNALRTGFGIQTTQFISFLSSLRVRRSDLLQKRSFLKEEN 749

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q  P WDD S +FL+MTLK + + ++S+S   +  GDLF+AFNS D SE+V L
Sbjct: 750  IDWYGSDQSLPTWDDRSSRFLAMTLKVEREENESSSEFSSLNGDLFVAFNSADQSERVIL 809

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPL---EAGLATYEMKSHSCILFEARSL 201
            PP   +  W+ LVDTA+PFPGFF+ DG P+    AGL TYEMKSHSC+LFEA+ L
Sbjct: 810  PPPPPEMVWLRLVDTAVPFPGFFSRDGEPVLEQMAGLVTYEMKSHSCVLFEAKKL 864


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score = 1084 bits (2804), Expect = 0.0
 Identities = 533/832 (64%), Positives = 646/832 (77%), Gaps = 5/832 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            +G+RV     KL L++    G+  K  + +S   H LKAL+TS +S+++ ++ V TY F 
Sbjct: 33   HGKRVLCRLRKLELEEINFSGRSRKTDQEVSKRTHRLKALSTSGISLIQTAKTVPTYLFR 92

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             + GGQ+KV ++K +N KY + VE    E  +   EL M+WGLFRSD++CFMPLD  +  
Sbjct: 93   TDTGGQVKVLVEK-TNGKYKVLVEALPSELSDAHAELVMVWGLFRSDASCFMPLDLNRRG 151

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K STIETPF +     + VELDF+ SLAPFY+S  +KSQL S++K S IRSHR T+F
Sbjct: 152  ADGKSSTIETPFVQGPSNKVTVELDFEASLAPFYISFYMKSQLVSDMKSSEIRSHRNTSF 211

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVG  SG P+PLG+S+  DG +NFA FSR+ +SVVLCLY D   +KP+LEIDLDPYVN
Sbjct: 212  VVPVGLTSGHPAPLGLSYQPDGTMNFALFSRSAKSVVLCLYDDISVEKPSLEIDLDPYVN 271

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFL 1797
            RSGDIWHA +DCSLPF  YGYRC+  T  K   VLLDPY++VI        G  L  K+L
Sbjct: 272  RSGDIWHAALDCSLPFKTYGYRCKVATSGKEEMVLLDPYSKVIRSVIPRQGGSKLRPKYL 331

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            G+LC+EP +DWSGD  PSLP+E LIIYRLNVT FT D SSKLP D+AGTF+GI+EKL HF
Sbjct: 332  GELCKEPGYDWSGDVPPSLPLENLIIYRLNVTEFTKDGSSKLPDDLAGTFSGITEKLSHF 391

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEPIFPF+EQKGPYFP HFFSPG+++G S DP +V  SMK+MVKKLHA+GI
Sbjct: 392  KHLGVNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVINSMKDMVKKLHANGI 451

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            E+ LEVVFTH +E A L  +DNSS+YS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVI
Sbjct: 452  EIFLEVVFTHIAEDAPLMHVDNSSHYSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVI 510

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFHIDGF FVNASSL++G HGE LSRPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  
Sbjct: 511  EFHIDGFVFVNASSLSKGFHGEILSRPPLVEAIAFDPILSKVKIIADYWDALANDSKEIL 570

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHW++WAE+N +FC D+R+FLRG+G +S+LATRLCGSGD+F  GRGPAFSFN+I RN G
Sbjct: 571  FPHWRKWAEINTRFCDDIRDFLRGKGLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFG 630

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            LTLVDLVSFS S++  + SWNCGEEGP    +VLETRLKQ+RNFLF+LFISLGVPVLNMG
Sbjct: 631  LTLVDLVSFSSSEVASELSWNCGEEGPMTNNIVLETRLKQVRNFLFILFISLGVPVLNMG 690

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GG PV+  RK  DW+ LK+GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEEN
Sbjct: 691  DECGQSSGGFPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEEN 750

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q PPKWDD S +FL+MTLK DA+ SQ TS S N GGDLF+AFNS   SE V L
Sbjct: 751  IQWHGSDQSPPKWDDPSSRFLAMTLKADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVIL 808

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            PP   D  W  LVDT LPFPGFF   G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 809  PPPPTDMVWHRLVDTGLPFPGFFAEKGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
 ref|XP_015168040.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
          Length = 878

 Score = 1083 bits (2800), Expect = 0.0
 Identities = 532/832 (63%), Positives = 638/832 (76%), Gaps = 5/832 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     KL L+     G      +      H  KAL+ S +S+V  ++ V TY F 
Sbjct: 32   NRGKIVCSLRKLELEDMNFSGIGRNNDQEAPRRAHRRKALSASRISLVPSAKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IGGQ+KV +++ +N KY + VEV  LE      EL M+WGLFRSD++CFMPLD  +  
Sbjct: 92   TDIGGQVKVLVER-TNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K ST+ETPF +   G + VELDF+ SLAPFY+S  +KSQL S+++ S IRSHR TNF
Sbjct: 151  ADGKSSTVETPFVQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVG  SG P+PLGISF  DG +NFA FSR+  SVVLCLY D   +KP+LEIDLDPY+N
Sbjct: 211  VVPVGLSSGHPAPLGISFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYIN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFL 1797
            RSGDIWHA +DCSLPF  YGYRC+  T  KG  VLLDPYA+VI        G ++  K+L
Sbjct: 271  RSGDIWHAALDCSLPFKTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYL 330

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            G+LC EP +DWSGD  PSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GISEK HHF
Sbjct: 331  GELCLEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHF 390

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEPIFPFDEQKGPYFPWHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GI
Sbjct: 391  KDLGVNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGI 450

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            EV LEVVFTHT+E A L  +DN SY  + GG+ L  +NALNCNYPIVQQMIL+ LR+WVI
Sbjct: 451  EVFLEVVFTHTAEDAPLMNVDNFSY-CIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVI 509

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFHIDGF FVNASSL RG +GE LSRPPL+EAIAFDP+LSKVK+IAD+W+P + ++KE  
Sbjct: 510  EFHIDGFVFVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENL 569

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I RN G
Sbjct: 570  FPHWRRWAEINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSG 629

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            LTLVDLVSFS +++  + SWNCG+EG T   +VLE RLKQ+RNFLF+LFISLGVPVLNMG
Sbjct: 630  LTLVDLVSFSSNEVASELSWNCGQEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMG 689

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GGSP Y  RK L WN LK+GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEEN
Sbjct: 690  DECGQSSGGSPAYDARKSLGWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEEN 749

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q PPKWD  S KFL+MTLK DA+ SQ+    +   GDLF+AFN    SE V L
Sbjct: 750  IQWHGSDQSPPKWDGPSSKFLAMTLKADAEVSQTLVSDI--VGDLFVAFNGAGDSEIVIL 807

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            PP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 808  PPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score = 1082 bits (2799), Expect = 0.0
 Identities = 535/831 (64%), Positives = 644/831 (77%), Gaps = 5/831 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMM 2499
            G+RV     KL L++    G+  K  + +S   H LKAL+ S +S+++ ++ V TY F  
Sbjct: 34   GKRVVCRLRKLELEEMNFSGRSRKIDQEVSKRSHRLKALSASGISLIQTAKRVPTYLFRT 93

Query: 2498 EIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSA 2319
            +IGGQ+KV ++K +N KY + VEV   E  +   EL M+WGLFRSD++CFMPLD  +  A
Sbjct: 94   DIGGQVKVLVEK-TNGKYKVLVEVLPSELSDAHAELVMVWGLFRSDASCFMPLDLNRHGA 152

Query: 2318 DSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFI 2139
            D K STIETPF +     + VELDF+ SLAPFY+S  +KSQL S++K S IRSHR T F+
Sbjct: 153  DGKSSTIETPFVQGPSDKVTVELDFEASLAPFYISFYMKSQLVSDMKSSEIRSHRNTTFV 212

Query: 2138 VPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNR 1959
            VPVG  SG P+PLG+S   DG +NFA FSR+ +SVVLCLY D   +KP+LEIDLDPYVNR
Sbjct: 213  VPVGLTSGHPAPLGLSCQPDGTMNFALFSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNR 272

Query: 1958 SGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLG 1794
            SGDIWHA +DCSLP   YGYRC+  T  K   VLLDPY++VI        G  L  K+LG
Sbjct: 273  SGDIWHAALDCSLPIKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSKLRPKYLG 332

Query: 1793 KLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFK 1614
            +LC+EP +DWSGD  PSL ME+LIIYRLNVT FT D SSKLP D+AGTF+GI+EK  HFK
Sbjct: 333  ELCKEPGYDWSGDVPPSLHMEKLIIYRLNVTEFTNDGSSKLPDDLAGTFSGITEKWSHFK 392

Query: 1613 NLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIE 1434
            +LGVNA+LLEPIFPF+EQKGPYFP HFFSPG+++G SGDP +V KSMK+MVKKLHA+GIE
Sbjct: 393  HLGVNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGHSGDPLSVIKSMKDMVKKLHANGIE 452

Query: 1433 VLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIE 1254
            + LEVVFTHT+E A L  +DNSSYYS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIE
Sbjct: 453  IFLEVVFTHTAEDAPLMHVDNSSYYSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIE 511

Query: 1253 FHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAF 1074
            FHIDGF F+NASSL +G HGE LSRPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  F
Sbjct: 512  FHIDGFVFINASSLLKGFHGEILSRPPLVEAIAFDPILSKVKIIADYWDALANDSKEILF 571

Query: 1073 PHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGL 894
            PHW++WAE+N +FC D+R+FLRG+G +S LATRLCGSGD+F  GRGPAFSFN+I RN GL
Sbjct: 572  PHWRKWAEINKRFCDDIRDFLRGKGLLSGLATRLCGSGDIFSGGRGPAFSFNYIARNFGL 631

Query: 893  TLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGD 714
            TLVDLVSFS S++  + SWNCGEEGPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGD
Sbjct: 632  TLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGD 691

Query: 713  ECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENI 534
            ECGQS+ GSPV+  RK  DW+ LK+GFGIQ TQFISFL+SL++RRSDLLQ R+FL+EENI
Sbjct: 692  ECGQSSRGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRSDLLQKRTFLEEENI 751

Query: 533  EWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLP 354
            +W G++Q PPKWDD S +FL+MTLK DA+ SQ TS S N GGDLF+AFNS   SE V LP
Sbjct: 752  QWHGSDQSPPKWDDPSSRFLAMTLKADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVILP 809

Query: 353  PLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            P   D  W  LVDTALPFP FF   G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 810  PPPTDMVWHRLVDTALPFPEFFAEKGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum]
 gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score = 1081 bits (2795), Expect = 0.0
 Identities = 531/832 (63%), Positives = 637/832 (76%), Gaps = 5/832 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     KL L+     G      +      H  KAL+ S +S+V  ++ V TY F 
Sbjct: 32   NRGKIVCSLRKLELEDMNFSGIGRNNDQEAPRRAHRRKALSASRISLVPSAKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IGGQ+KV +++ +N KY + VEV  LE      EL M+WGLFRSD++CFMPLD  +  
Sbjct: 92   TDIGGQVKVLVER-TNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K ST+ETPF +   G + VELDF+ SLAPFY+S  +KSQL S+++ S IRSHR TNF
Sbjct: 151  ADGKSSTVETPFVQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVG  SG P+PLGISF  DG +NFA FSR+  SVVLCLY D   +KP+LEIDLDPY+N
Sbjct: 211  VVPVGLSSGHPAPLGISFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYIN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFL 1797
            RSGDIWHA +DCSLPF  YGYRC+  T  KG  VLLDPYA+VI        G ++  K+L
Sbjct: 271  RSGDIWHAALDCSLPFKTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYL 330

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            G+LC EP +DWSGD  PSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GISEK HHF
Sbjct: 331  GELCLEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHF 390

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEPIFPFDEQKGPYFPWHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GI
Sbjct: 391  KDLGVNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGI 450

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            EV LEVVFTHT+E A L  +DN SY  + GG+ L  +NALNCNYPIVQQMIL+ LR+WVI
Sbjct: 451  EVFLEVVFTHTAEDAPLMNVDNFSY-CIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVI 509

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFHIDGF FVNASSL RG +GE LSRPPL+EAIAFDP+LSKVK+IAD+W+P + ++KE  
Sbjct: 510  EFHIDGFVFVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENL 569

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I RN G
Sbjct: 570  FPHWRRWAEINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSG 629

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            LTLVDLVSFS +++  + SWNCG+EG T   +VLE RLKQ+RNFLF+LFISLGVPVLNMG
Sbjct: 630  LTLVDLVSFSSNEVASELSWNCGQEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMG 689

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GG P Y  RK L WN LK+GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEEN
Sbjct: 690  DECGQSSGGPPAYDARKSLGWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEEN 749

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q PPKWD  S KFL+MTLK DA+ SQ+    +   GDLF+AFN    SE V L
Sbjct: 750  IQWHGSDQSPPKWDGPSSKFLAMTLKADAEVSQTLVSDI--VGDLFVAFNGAGDSEIVIL 807

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            PP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 808  PPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Solanum pennellii]
          Length = 866

 Score = 1080 bits (2794), Expect = 0.0
 Identities = 528/832 (63%), Positives = 638/832 (76%), Gaps = 5/832 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     KL L+     G      +      H LKAL+ S +S+V  ++ V TY F 
Sbjct: 32   NRGKIVCSPRKLELEDMNFSGIGRNNDQEAPRRAHRLKALSGSRISLVPSAKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
              IGGQ+KVF++K +N KY + VEV  LE  +   EL M+WGLFRSD++CFMPLD  +  
Sbjct: 92   TAIGGQVKVFVEK-TNGKYKVLVEVLPLELSDAHSELVMVWGLFRSDASCFMPLDLNRRG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K ST+ETPF +   G + VELDF+ SL PFY+S  +KSQL S+++ S IRSHR TNF
Sbjct: 151  ADGKSSTVETPFVQGPSGKVTVELDFEVSLVPFYISFYMKSQLVSDMENSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVG  SG P+PLGISF  DG +NFA +SR+ + VVLCLY D   +KP+LEIDLDPY+N
Sbjct: 211  VVPVGLSSGHPAPLGISFQPDGYVNFALYSRSAKGVVLCLYDDISVEKPSLEIDLDPYIN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFL 1797
            RSGDIWHA +DCSLPF  YGYR +  T  KG  VLLDPYA+VI        G ++  K+L
Sbjct: 271  RSGDIWHAALDCSLPFKTYGYRFKAATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYL 330

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            G+LC EP +DWSGD  PSLPME+LIIYR+NVT FT DKSSKLP ++AGTF+GISEK HH 
Sbjct: 331  GELCLEPGYDWSGDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHL 390

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEPIFPFDEQKGPY+PWHFFSPG++YGPSGDP +V K+MK+MVKKLHA+GI
Sbjct: 391  KDLGVNAMLLEPIFPFDEQKGPYYPWHFFSPGNMYGPSGDPLSVIKTMKDMVKKLHANGI 450

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            EV LEVVFTHT+E A L  +DN SY  + GG+DL  +NALNCN PIVQQMIL+ LR+WVI
Sbjct: 451  EVFLEVVFTHTAEDAPLMNVDNFSY-CIKGGQDLNIQNALNCNCPIVQQMILDCLRHWVI 509

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFHIDGF FVNASSL RG +GE LSRPPL+EAIAFD +LSKVK+IAD+W+P + ++ E  
Sbjct: 510  EFHIDGFVFVNASSLLRGFNGEILSRPPLVEAIAFDLILSKVKMIADNWNPLTNDSTENL 569

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I RN G
Sbjct: 570  FPHWRRWAEINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSG 629

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            LTLVDLVSFS S++  + SWNCG+EG T  ++VLE RLKQ+RNFLF+LFISLGVPVLNMG
Sbjct: 630  LTLVDLVSFSNSEVASELSWNCGQEGATTNSIVLERRLKQVRNFLFILFISLGVPVLNMG 689

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GGSP Y  RK LDWN LK+GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEEN
Sbjct: 690  DECGQSSGGSPAYDARKSLDWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEEN 749

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q PPKWDD S KFL+MTLK DA+ S +    +  GGDLF+AFN    SE V L
Sbjct: 750  IQWHGSDQSPPKWDDPSSKFLAMTLKADAEVSHTLLSDI--GGDLFVAFNGAGDSESVIL 807

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            PP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 808  PPPPTDMVWYRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCVLFEAQRL 859


>ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
 ref|XP_009761455.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
          Length = 863

 Score = 1078 bits (2788), Expect = 0.0
 Identities = 531/831 (63%), Positives = 643/831 (77%), Gaps = 5/831 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMM 2499
            G+RV    +KL L +    G+  K  + +S   H LKAL+ S +S+++ ++ V TY F  
Sbjct: 34   GKRVACRLSKLELGEMNFSGRSRKTDQEVSKRTHRLKALSASGISLIQTAKRVPTYLFRT 93

Query: 2498 EIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSA 2319
            +IGG +KV ++K +N KY + VEV   EH +   EL M+WGLFRSD++CFMPLD  +  A
Sbjct: 94   DIGGHVKVLVEK-TNGKYIVLVEVLPSEHSDAHAELVMLWGLFRSDASCFMPLDLNRRGA 152

Query: 2318 DSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFI 2139
            D + ST+ETPF +     + VELDF+ SLAPFY+S  +KSQL S++K S IRSHR TNF+
Sbjct: 153  DGQSSTLETPFVQGPSDKVTVELDFEASLAPFYISFYMKSQLVSDMKRSEIRSHRNTNFV 212

Query: 2138 VPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNR 1959
            VPVG  SG PSPLG+S   DG +NFA FSR+ +SVVLCLY D   + P+LEIDLDPYVNR
Sbjct: 213  VPVGLTSGHPSPLGLSCQPDGTMNFALFSRSAKSVVLCLYDDISVEMPSLEIDLDPYVNR 272

Query: 1958 SGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLG 1794
            SGDIWHA +DCSL F  YGYRC+  T  K   VLLDPY++VI        G  L  K+LG
Sbjct: 273  SGDIWHAALDCSLSFKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSKLHPKYLG 332

Query: 1793 KLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFK 1614
            +LC+EP +DWS D  PSLPME+LI+YRLNVT FT D SSKLP D+AGTF+GI+EK  HFK
Sbjct: 333  ELCKEPGYDWSDDVPPSLPMEKLIVYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFK 392

Query: 1613 NLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIE 1434
            +LGVNA+LLEPIFPF+EQKGPYFP HFFSPG+++G S DP +V KSMK+MVKKLHA+GIE
Sbjct: 393  HLGVNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIE 452

Query: 1433 VLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIE 1254
            + LEV+FTHT+E A L  IDNS YYS+ GG D+  + ALNCNYPIVQQMIL+ LRYWVIE
Sbjct: 453  IFLEVIFTHTAEDAPLMHIDNSCYYSIKGG-DMNIQFALNCNYPIVQQMILDCLRYWVIE 511

Query: 1253 FHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAF 1074
            FHIDGF FVNASSL +G HGE LSRP L+EAIAFDP+LSKVK+IAD WD  + ++KE  F
Sbjct: 512  FHIDGFVFVNASSLLKGFHGEILSRPQLVEAIAFDPILSKVKIIADYWDALANDSKEILF 571

Query: 1073 PHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGL 894
            PHW++WAE+N +FC D+R+FLRG+G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GL
Sbjct: 572  PHWRKWAEINTRFCDDIRDFLRGKGLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGL 631

Query: 893  TLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGD 714
            TLVDLVSFS S++  + SWNCGEEGPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGD
Sbjct: 632  TLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGD 691

Query: 713  ECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENI 534
            ECGQS+GGSPV+  RK  DW+ LK+GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI
Sbjct: 692  ECGQSSGGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENI 751

Query: 533  EWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLP 354
            +W G++Q PPKWDD S +FL+M+LK DA+ SQ TS S N GGDLF+AFNS   SE V LP
Sbjct: 752  QWHGSDQSPPKWDDPSSRFLAMSLKADAEGSQ-TSVSDN-GGDLFVAFNSAGESESVILP 809

Query: 353  PLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            P   D  W  LVDTALPFPGFF   G P+E GL  YEMK HSC+LFEA+ L
Sbjct: 810  PPPTDVVWHRLVDTALPFPGFFAEKGTPVEDGLVAYEMKPHSCLLFEAQKL 860


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
 ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score = 1078 bits (2788), Expect = 0.0
 Identities = 534/831 (64%), Positives = 643/831 (77%), Gaps = 5/831 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMM 2499
            G+RV     KL L++    G+  K  + +S   H LKAL+ S +S+++ ++ V TY F  
Sbjct: 34   GKRVVCRLRKLELEEMNFSGRSRKIDQEVSKRTHRLKALSASGISLIQTAKRVPTYLFRT 93

Query: 2498 EIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSA 2319
            +IGGQ+KV ++K +N KY + VEV   E  +   EL M+WGLFRSD++CFMPLD  +  A
Sbjct: 94   DIGGQVKVLVEK-TNGKYKVLVEVLPSELSDAHAELVMVWGLFRSDASCFMPLDLNRHGA 152

Query: 2318 DSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFI 2139
            D K STIETPF +     + VELDF+ SLAPFY+S  +KSQL S++K S IRSHR T F+
Sbjct: 153  DGKSSTIETPFVQGPSDKVTVELDFEASLAPFYISFYMKSQLVSDMKSSEIRSHRNTTFV 212

Query: 2138 VPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNR 1959
            VPVG  SG P+PLG+S   DG +NFA FSR+ +SVVLCLY D   +KP+LEIDLDPYVNR
Sbjct: 213  VPVGLTSGHPAPLGLSCQPDGTMNFALFSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNR 272

Query: 1958 SGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLG 1794
            SGDIWHA +DCSLP   YGYRC+  T  K   VLLDPY++VI        G  L  K+LG
Sbjct: 273  SGDIWHAALDCSLPIKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSKLRPKYLG 332

Query: 1793 KLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFK 1614
            +LC+EP +DWS D  PSL ME+LIIYRLNVT FT D SSKLP D+AGTF+GI+EK  HFK
Sbjct: 333  ELCKEPGYDWSVDVPPSLHMEKLIIYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFK 392

Query: 1613 NLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIE 1434
            +LGVNA+LLEPIFPF+EQKGPYFP HFFSPG+++G SGDP +V KSMK+MVKKLHA+GIE
Sbjct: 393  HLGVNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGRSGDPLSVIKSMKDMVKKLHANGIE 452

Query: 1433 VLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIE 1254
            + LEVVFTHT+E A L  +DNSSYYS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIE
Sbjct: 453  IFLEVVFTHTAEDAPLMHVDNSSYYSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIE 511

Query: 1253 FHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAF 1074
            FHIDGF F+NASSL +G HGE  SRPPL+EAIAFDP+LSKVK+IAD WD  + ++KE  F
Sbjct: 512  FHIDGFVFINASSLLKGFHGEIQSRPPLVEAIAFDPILSKVKIIADYWDALANDSKEILF 571

Query: 1073 PHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGL 894
            PHW++WAE+N +FC D+R+FLRG+G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GL
Sbjct: 572  PHWRKWAEINTRFCDDIRDFLRGKGLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGL 631

Query: 893  TLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGD 714
            TLVDLVSFS S++  + SWNCGEEGPT   +VLETRLKQ+RNFLF+LFISLGVPVLNMGD
Sbjct: 632  TLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGD 691

Query: 713  ECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENI 534
            ECGQS+ GSPV+  RK  DW+ LK+GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI
Sbjct: 692  ECGQSSRGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENI 751

Query: 533  EWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLP 354
            +W G++Q PPKWDD S +FL+MTLK DA+ SQ TS S N GGDLF+AFNS   SE V LP
Sbjct: 752  QWHGSDQSPPKWDDPSSRFLAMTLKADAEDSQ-TSVSDN-GGDLFVAFNSAGESESVILP 809

Query: 353  PLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            P   D  W  LVDTALPFP FF   G P+E GL  YEMKSHSC+LFEA+ L
Sbjct: 810  PPPTDMVWHRLVDTALPFPEFFAEKGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
 ref|XP_016475508.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score = 1076 bits (2783), Expect = 0.0
 Identities = 530/831 (63%), Positives = 642/831 (77%), Gaps = 5/831 (0%)
 Frame = -1

Query: 2678 GRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFMM 2499
            G+RV    +KL L +    G+  K  + +S   H LKAL  S +S+++ ++ V TY F  
Sbjct: 34   GKRVACRLSKLELGEMNFSGRSRKTDQEVSKRTHRLKALPASGISLIQTAKRVPTYLFRT 93

Query: 2498 EIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSSA 2319
            +IGG +KV ++K +N KY + VEV   EH +   EL M+WGLFRSD++CFMPLD  +  A
Sbjct: 94   DIGGHVKVLVEK-TNGKYIVLVEVLPSEHSDAHAELVMLWGLFRSDASCFMPLDLNRRGA 152

Query: 2318 DSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNFI 2139
            D + ST+ETPF +     + VELDF+ SLAPFY+S  +KSQL S++K S IRSHR TNF+
Sbjct: 153  DGQSSTLETPFVQGPSDKVTVELDFEASLAPFYISFYMKSQLVSDMKRSEIRSHRNTNFV 212

Query: 2138 VPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVNR 1959
            VPVG  SG PSPLG+S   DG +NFA FSR+ +SVVLCLY D   + P+LEIDLDPYVNR
Sbjct: 213  VPVGLTSGHPSPLGLSCQPDGTMNFALFSRSAKSVVLCLYDDISVEMPSLEIDLDPYVNR 272

Query: 1958 SGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLG 1794
            SGDIWHA +DCSL F  YGYRC+  T  K   VLLDPY++VI        G  L  K+LG
Sbjct: 273  SGDIWHAALDCSLSFKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSKLHPKYLG 332

Query: 1793 KLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFK 1614
            +LC+EP +DWS D  PSLPME+LI+YRLNVT FT D SSKLP D+AGTF+GI+EK  HFK
Sbjct: 333  ELCKEPGYDWSDDVPPSLPMEKLIVYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFK 392

Query: 1613 NLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIE 1434
            +LGVNA+LLEPIFPF+EQKGPYFP HFFSPG+++G S DP +V KSMK+MVKKLHA+GIE
Sbjct: 393  HLGVNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIE 452

Query: 1433 VLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIE 1254
            + LEV+FTHT+E A L  IDNS YYS+ GG D+  + ALNCNYPIVQQMIL+ LRYWVIE
Sbjct: 453  IFLEVIFTHTAEDAPLMHIDNSCYYSIKGG-DMNIQFALNCNYPIVQQMILDCLRYWVIE 511

Query: 1253 FHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETAF 1074
            FHIDGF FVNASSL +G HGE LSRP L+EAIAFDP+LSKVK+IAD WD  + ++KE  F
Sbjct: 512  FHIDGFVFVNASSLLKGFHGEILSRPQLVEAIAFDPILSKVKIIADYWDALANDSKEILF 571

Query: 1073 PHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVGL 894
            PHW++WAE+N +FC D+R+FLRG+G +S+LATRLCGSGD+F  GRGPAFSFN+I RN GL
Sbjct: 572  PHWRKWAEINTRFCDDIRDFLRGKGLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGL 631

Query: 893  TLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMGD 714
            TLVDLVSFS S++  + SWNCGEEGPT   +VLETR+KQ+RNFLF+LFISLGVPVLNMGD
Sbjct: 632  TLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRVKQVRNFLFILFISLGVPVLNMGD 691

Query: 713  ECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEENI 534
            ECGQS+GGSPV+  RK  DW+ LK+GFGIQ TQFISFL+SL++RRSDLLQ R+FLKEENI
Sbjct: 692  ECGQSSGGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENI 751

Query: 533  EWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTLP 354
            +W G++Q PPKWDD S +FL+M+LK DA+ SQ TS S N GGDLF+AFNS   SE V LP
Sbjct: 752  QWHGSDQSPPKWDDPSSRFLAMSLKADAEGSQ-TSVSDN-GGDLFVAFNSAGESESVILP 809

Query: 353  PLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            P   D  W  LVDTALPFPGFF   G P+E GL  YEMK HSC+LFEA+ L
Sbjct: 810  PPPTDVVWHRLVDTALPFPGFFAEKGTPVEDGLVAYEMKPHSCLLFEAQKL 860


>gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]
          Length = 862

 Score = 1074 bits (2778), Expect = 0.0
 Identities = 531/833 (63%), Positives = 644/833 (77%), Gaps = 6/833 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     KL L++    G   K  +++S   H LKAL+ + +S+V   + V TY F 
Sbjct: 32   NRGKIVCSVKKLELEEMNFSGIGRKNYQQVSGRFHQLKALSATGISLVPTVKKVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IGGQ+KV+++K +N KY + VEV  +E  +   EL ++ G+FRSD +CFMPLD  +  
Sbjct: 92   TDIGGQVKVYVEK-TNGKYKVLVEVLPVELSDAHAELVIVCGVFRSDVSCFMPLDLNRCG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K STIETPF +   G + VELDF+ SLAP Y+S  +KSQL ++++ S IRSHR TNF
Sbjct: 151  ADGKSSTIETPFVQGPSGKVTVELDFEASLAPCYISFYIKSQLVTDMESSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVGF SG PSPLG+SF  DG +NFA FS + +SVVLCLY DT A+KP LEIDLDPYVN
Sbjct: 211  VVPVGFSSGHPSPLGLSFLPDGSLNFALFSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKF 1800
            RSGDIWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+
Sbjct: 271  RSGDIWHAALDCSLLFKTYGYRCKVATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKY 330

Query: 1799 LGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHH 1620
            LG+LC+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HH
Sbjct: 331  LGELCQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHH 390

Query: 1619 FKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASG 1440
            FK+LGVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+G
Sbjct: 391  FKDLGVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSVKDMVKKLHANG 450

Query: 1439 IEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWV 1260
            IEV LEVVFTHT++ A L  +DN+SYY + GG DL  +NALNCNYP+VQQMIL+ LR+WV
Sbjct: 451  IEVFLEVVFTHTAQDAPLMHVDNTSYY-IKGG-DLNIQNALNCNYPVVQQMILDCLRHWV 508

Query: 1259 IEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKET 1080
            IEF IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VK+IAD+W+P + ++KE 
Sbjct: 509  IEFRIDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKEN 568

Query: 1079 AFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNV 900
             FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I+RN 
Sbjct: 569  LFPHWRRWAEINLRFCADIRDFLRGEGLLSNLATRLCGSGDIFSGGRGPAFSFNYISRNF 628

Query: 899  GLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNM 720
            GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLF+LFISLGVPVLNM
Sbjct: 629  GLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFILFISLGVPVLNM 688

Query: 719  GDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEE 540
            GDECGQS+GGSP Y  RK  DWN LK+GFGIQ TQFISFLS+L++RRSDLLQ  +FLKEE
Sbjct: 689  GDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFISFLSNLRMRRSDLLQKSTFLKEE 748

Query: 539  NIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVT 360
            NI W G +Q PPKWDD S KFL+MTLK DA+ SQ+    +  GGDLF+AFNS   SE V 
Sbjct: 749  NIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSEI--GGDLFVAFNSAGDSECVI 806

Query: 359  LPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            LPP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA  L
Sbjct: 807  LPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAHRL 859


>ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Solanum lycopersicum]
          Length = 866

 Score = 1074 bits (2778), Expect = 0.0
 Identities = 523/832 (62%), Positives = 637/832 (76%), Gaps = 5/832 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     KL L+     G      +      H LKAL+ S +S+V  ++ V TY F 
Sbjct: 32   NRGKIVCSLRKLELEDMNFSGISRNNDQEAPRRAHRLKALSGSRISLVPSAKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IG Q+KVF++K +N KY + VEV  LE  +   EL M+WGLFRSD++CFMPLD  +  
Sbjct: 92   TDIGCQVKVFVEK-TNGKYKVLVEVLPLELSDAHSELVMVWGLFRSDASCFMPLDLNRRG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K ST+ETPF +   G + VELDF+ SL PFY+S  +KSQL S+++ S IRSHR TNF
Sbjct: 151  ADGKSSTVETPFVQGPSGKVTVELDFEVSLVPFYISFYMKSQLVSDMENSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVG  SG P+PLGISF  DG +NFA +SR+ + VVLCLY D   +KP+LEIDLDPY+N
Sbjct: 211  VVPVGLSSGHPAPLGISFQPDGYVNFALYSRSAKGVVLCLYDDISVEKPSLEIDLDPYIN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFL 1797
            +SGDIWHA +DCSLPF  YGYR +  T  KG  VLLDPYA+VI        G ++  K+L
Sbjct: 271  QSGDIWHAALDCSLPFKTYGYRFKAATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYL 330

Query: 1796 GKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHF 1617
            G+LC EP +DWSGD  PSLPME+LIIYR+NVT FT DKSSKLP ++AGTF+GISEK HH 
Sbjct: 331  GELCLEPGYDWSGDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHL 390

Query: 1616 KNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGI 1437
            K+LGVNA+LLEPIFPFDEQKGPY+PWHFFSPG++YGPSGDP +V KSMK+MVKKLHA+GI
Sbjct: 391  KDLGVNAMLLEPIFPFDEQKGPYYPWHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGI 450

Query: 1436 EVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVI 1257
            EV LEVVFTHT+E A L  +DN SY  + G +D+  +NALNCNYPIVQQMIL+ LR+WVI
Sbjct: 451  EVFLEVVFTHTAEDAPLMNVDNFSY-CIKGSQDMNIQNALNCNYPIVQQMILDCLRHWVI 509

Query: 1256 EFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKETA 1077
            EFHIDGF FV+ASSL RG +GE LSRPPL+EAIAFDP+LSK K+IAD+W+P + ++ E  
Sbjct: 510  EFHIDGFVFVDASSLLRGFNGEILSRPPLVEAIAFDPILSKAKMIADNWNPLTNDSTENL 569

Query: 1076 FPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNVG 897
            FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I RN G
Sbjct: 570  FPHWRRWAEINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSG 629

Query: 896  LTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNMG 717
            LTLVDLVSFS S++  + SWNCG+EG T  ++VLE RLKQ+RNFLF+LFISLGVPVLNMG
Sbjct: 630  LTLVDLVSFSNSEVASELSWNCGQEGATTNSIVLERRLKQVRNFLFILFISLGVPVLNMG 689

Query: 716  DECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEEN 537
            DECGQS+GGSP Y  RK L+WN LK+GFG Q+ QFISFLS+L++RRSDLLQ R+FLKEEN
Sbjct: 690  DECGQSSGGSPAYDARKSLNWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEEN 749

Query: 536  IEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVTL 357
            I+W G++Q PP WDD S KFL+MTLK DA+ S +    +  GGDLF+AFN    SE V L
Sbjct: 750  IQWHGSDQSPPVWDDPSSKFLAMTLKADAEVSHTLLSDI--GGDLFVAFNGAGDSESVIL 807

Query: 356  PPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            PP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 808  PPPPTDMVWYRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCVLFEAQRL 859


>gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]
          Length = 862

 Score = 1071 bits (2770), Expect = 0.0
 Identities = 530/833 (63%), Positives = 644/833 (77%), Gaps = 6/833 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     K  L++    G   K  +++S   H LKAL+ S +S+V   + V TY F 
Sbjct: 32   NRGKIVCSVKKRELEEMNFSGIGRKNDQQVSGRFHQLKALSASGISLVPTVKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IGGQ+KV+++K +N KY + VEV  +E  +   EL ++ G+FRSD++CFMPLD  +  
Sbjct: 92   TDIGGQVKVYVEK-TNGKYKVLVEVLPVELSDAHAELVIVCGVFRSDASCFMPLDLNRCG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K STIETPF +   G + VELDF+ SLAP Y+S  +KSQL ++++ S IRSHR TNF
Sbjct: 151  ADGKSSTIETPFVQGPSGKVTVELDFEASLAPCYISFYIKSQLVTDMESSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVGF SG PSPLG+SF  DG +NFA FS + +SVVLCLY DT A+KP LEIDLDPYVN
Sbjct: 211  VVPVGFSSGHPSPLGLSFLPDGSLNFALFSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKF 1800
            RSGDIWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+
Sbjct: 271  RSGDIWHAALDCSLLFKTYGYRCKAATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKY 330

Query: 1799 LGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHH 1620
            LG+LC+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HH
Sbjct: 331  LGELCQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHH 390

Query: 1619 FKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASG 1440
            FK+LGVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+G
Sbjct: 391  FKDLGVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSVKDMVKKLHANG 450

Query: 1439 IEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWV 1260
            IEV LEVVFTHT++ A L  +DN+SY  V GG DL  +NALNCNYP+VQQMIL+ LR+WV
Sbjct: 451  IEVFLEVVFTHTAQDAPLMHVDNTSY-CVKGG-DLNIQNALNCNYPVVQQMILDCLRHWV 508

Query: 1259 IEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKET 1080
            IEF IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VK+IAD+W+P + ++KE 
Sbjct: 509  IEFRIDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKEN 568

Query: 1079 AFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNV 900
             FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I+RN 
Sbjct: 569  LFPHWRRWAEINLRFCADIRDFLRGEGLLSNLATRLCGSGDIFSGGRGPAFSFNYISRNF 628

Query: 899  GLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNM 720
            GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLF+LFISLGVPVLNM
Sbjct: 629  GLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFILFISLGVPVLNM 688

Query: 719  GDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEE 540
            GDECGQS+GGSP Y  RK  DWN LK+GFGIQ TQFI+FLS+L++RRSDLLQ  +FLKEE
Sbjct: 689  GDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFIAFLSNLRMRRSDLLQKSTFLKEE 748

Query: 539  NIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVT 360
            NI W G +Q PPKWDD S KFL+MTLK DA+ SQ+    +  GGDLF+AFNS   SE V 
Sbjct: 749  NIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSDI--GGDLFVAFNSAGDSECVI 806

Query: 359  LPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            LPP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 807  LPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559779.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559780.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
          Length = 862

 Score = 1071 bits (2770), Expect = 0.0
 Identities = 530/833 (63%), Positives = 644/833 (77%), Gaps = 6/833 (0%)
 Frame = -1

Query: 2681 NGRRVTSDSNKLNLKQKTVHGQLMKPARRISYDHHVLKALATSDVSVVKMSENVATYRFM 2502
            N  ++     K  L++    G   K  +++S   H LKAL+ S +S+V   + V TY F 
Sbjct: 32   NRGKIVCSVKKRELEEMNFSGIGRKNDQQVSGRFHQLKALSASGISLVPTVKRVPTYLFR 91

Query: 2501 MEIGGQLKVFIKKKSNNKYGLHVEVEFLEHCEGQGELFMIWGLFRSDSTCFMPLDFKQSS 2322
             +IGGQ+KV+++K +N KY + VEV  +E  +   EL ++ G+FRSD++CFMPLD  +  
Sbjct: 92   TDIGGQVKVYVEK-TNGKYKVLVEVLPVELSDAHAELVIVCGVFRSDASCFMPLDLNRCG 150

Query: 2321 ADSKQSTIETPFTKNSVGTLAVELDFDESLAPFYVSILLKSQLGSNLKLSTIRSHRKTNF 2142
            AD K STIETPF +   G + VELDF+ SLAP Y+S  +KSQL ++++ S IRSHR TNF
Sbjct: 151  ADGKSSTIETPFVQGPSGKVTVELDFEASLAPCYISFYIKSQLVTDMESSEIRSHRNTNF 210

Query: 2141 IVPVGFGSGRPSPLGISFTDDGLINFAFFSRNVESVVLCLYADTRADKPALEIDLDPYVN 1962
            +VPVGF SG PSPLG+SF  DG +NFA FS + +SVVLCLY DT A+KP LEIDLDPYVN
Sbjct: 211  VVPVGFSSGHPSPLGLSFLPDGSLNFALFSHSAKSVVLCLYDDTSAEKPTLEIDLDPYVN 270

Query: 1961 RSGDIWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKF 1800
            RSGDIWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+
Sbjct: 271  RSGDIWHAALDCSLLFKTYGYRCKAATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKY 330

Query: 1799 LGKLCEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHH 1620
            LG+LC+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HH
Sbjct: 331  LGELCQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHH 390

Query: 1619 FKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASG 1440
            FK+LGVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+G
Sbjct: 391  FKDLGVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSIKDMVKKLHANG 450

Query: 1439 IEVLLEVVFTHTSELASLREIDNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWV 1260
            IEV LEVVFTHT++ A L  +DN+SY  V GG DL  +NALNCNYP+VQQMIL+ LR+WV
Sbjct: 451  IEVFLEVVFTHTAQDAPLMHVDNTSY-CVKGG-DLNIQNALNCNYPVVQQMILDCLRHWV 508

Query: 1259 IEFHIDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKLIADSWDPHSVETKET 1080
            IEF IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VK+IAD+W+P + ++KE 
Sbjct: 509  IEFRIDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKEN 568

Query: 1079 AFPHWKRWAEMNGKFCVDVRNFLRGQGPISNLATRLCGSGDLFLSGRGPAFSFNFITRNV 900
             FPHW+RWAE+N +FC D+R+FLRG+G +SNLATRLCGSGD+F  GRGPAFSFN+I+RN 
Sbjct: 569  LFPHWRRWAEINLRFCADIRDFLRGEGLLSNLATRLCGSGDIFSGGRGPAFSFNYISRNF 628

Query: 899  GLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFVLFISLGVPVLNM 720
            GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLF+LFISLGVPVLNM
Sbjct: 629  GLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFILFISLGVPVLNM 688

Query: 719  GDECGQSTGGSPVYTDRKPLDWNGLKSGFGIQVTQFISFLSSLKVRRSDLLQNRSFLKEE 540
            GDECGQS+GGSP Y  RK  DWN LK+GFGIQ TQFI+FLS+L++RRSDLLQ  +FLKEE
Sbjct: 689  GDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFIAFLSNLRMRRSDLLQKSTFLKEE 748

Query: 539  NIEWRGTEQFPPKWDDESCKFLSMTLKTDAQPSQSTSGSLNAGGDLFIAFNSGDHSEKVT 360
            NI W G +Q PPKWDD S KFL+MTLK DA+ SQ+    +  GGDLF+AFNS   SE V 
Sbjct: 749  NIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSDI--GGDLFVAFNSAGDSECVI 806

Query: 359  LPPLAVDATWVCLVDTALPFPGFFTVDGVPLEAGLATYEMKSHSCILFEARSL 201
            LPP   D  W  LVDTALPFPGFF   G P+E  L  YEMKSHSC+LFEA+ L
Sbjct: 807  LPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAQRL 859