BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00008322
         (822 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIN02757.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   362   e-120
gb|PIN00452.1| N-methylcoclaurine 3'-monooxygenase [Handroanthus...   349   e-118
gb|PIM98733.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   295   5e-94
gb|PIN05630.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   290   2e-92
gb|PIM98211.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   287   4e-91
ref|XP_012846401.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   286   1e-90
ref|XP_012837735.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   273   2e-85
ref|XP_011101194.2| probable (S)-N-methylcoclaurine 3'-hydroxyla...   251   1e-78
gb|EYU25066.1| hypothetical protein MIMGU_mgv1a021633mg, partial...   239   1e-72
ref|XP_012852332.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   239   2e-72
ref|XP_011083904.2| probable (S)-N-methylcoclaurine 3'-hydroxyla...   233   1e-70
gb|EYU45167.1| hypothetical protein MIMGU_mgv1a024523mg [Erythra...   228   2e-68
gb|EYU25065.1| hypothetical protein MIMGU_mgv1a019909mg, partial...   228   4e-68
ref|XP_012851911.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   228   7e-67
ref|XP_012846393.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   205   5e-60
ref|XP_011078628.1| probable (S)-N-methylcoclaurine 3'-hydroxyla...   201   1e-57
ref|XP_020549758.1| probable (S)-N-methylcoclaurine 3'-hydroxyla...   191   2e-55
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   188   8e-53
ref|XP_011084765.1| probable (S)-N-methylcoclaurine 3'-hydroxyla...   188   8e-53
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   188   8e-53

>gb|PIN02757.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 490

 Score =  362 bits (930), Expect = e-120
 Identities = 183/275 (66%), Positives = 222/275 (80%), Gaps = 3/275 (1%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGPN+  +   IS    +PH+AL++L+K+YGPL+SLRLGGQIVVVASTP IA+E+ KT
Sbjct: 27  LPPGPNLLIVLKNISQFSKSPHIALTNLSKMYGPLISLRLGGQIVVVASTPAIAEEVLKT 86

Query: 185 NNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIRT 364
           +NR+FSGRFLPSVYYNVPGT +ASLVMARECNDTWKFLRGIGQ  IFSS AIES + IRT
Sbjct: 87  HNRVFSGRFLPSVYYNVPGTKNASLVMARECNDTWKFLRGIGQKSIFSSMAIESTSPIRT 146

Query: 365 GQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFLN 544
            +V EM++FLR NK+GKVVNIE+++ ATFTNI +N++ASRNLF+ GGESE D KVM F N
Sbjct: 147 MKVMEMMKFLRNNKEGKVVNIENIISATFTNIATNVLASRNLFEPGGESEKDKKVMNFFN 206

Query: 545 EEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIK---ERSTAINDNST 715
            +II+KA+TFGL+D FP+L+ VDFW+KGRAM +YR IN  W DIIK   ER  +    ST
Sbjct: 207 -DIIEKATTFGLSDFFPVLERVDFWTKGRAMGIYRTINLIWDDIIKERRERGESAETKST 265

Query: 716 KDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           +D LDVL GH F  D I IL TEL++AG D+TTIT
Sbjct: 266 QDFLDVLIGHSFSNDEIGILLTELIVAGMDTTTIT 300


>gb|PIN00452.1| N-methylcoclaurine 3'-monooxygenase [Handroanthus impetiginosus]
          Length = 293

 Score =  349 bits (895), Expect = e-118
 Identities = 176/262 (67%), Positives = 211/262 (80%), Gaps = 3/262 (1%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGPN+  +   IS L  +PH+AL++L+K+YGPL+SLRLGGQIVVVASTP IA+E+ KT
Sbjct: 27  LPPGPNLLIVLKNISQLSKSPHIALTNLSKMYGPLISLRLGGQIVVVASTPAIAEEVLKT 86

Query: 185 NNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIRT 364
           +NR+FSGRFLPSVYYNVPGT HASLVMARECNDTWKFLRGIGQ  IFSS AIES + IRT
Sbjct: 87  HNRVFSGRFLPSVYYNVPGTKHASLVMARECNDTWKFLRGIGQKSIFSSMAIESTSPIRT 146

Query: 365 GQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFLN 544
            +V EM++FLR NK+GKVVNIE+++ ATFTNI +N++ASRNLF+ GGESE D KVM F N
Sbjct: 147 MKVMEMMKFLRNNKEGKVVNIENIISATFTNIATNVLASRNLFEPGGESEKDKKVMNFFN 206

Query: 545 EEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIK---ERSTAINDNST 715
            +II+KA+TFG +D FP+L+ VDFW+KGRAM +YR IN  WGDIIK   ER  +    ST
Sbjct: 207 -DIIEKATTFGFSDFFPVLERVDFWTKGRAMGIYRTINLIWGDIIKERRERGDSAETKST 265

Query: 716 KDLLDVLNGHGFPVDHIAILFT 781
           +D LDVL GH F  D I IL T
Sbjct: 266 QDFLDVLIGHSFSNDEIGILLT 287


>gb|PIM98733.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  295 bits (754), Expect = 5e-94
 Identities = 149/276 (53%), Positives = 200/276 (72%), Gaps = 4/276 (1%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGPN+ ++   +  LI  PH+AL  LAKIYGPL+S +LG Q++VVAS+PD A+E  KT
Sbjct: 26  LPPGPNVLRVLGYLPQLIYKPHIALQKLAKIYGPLLSFQLGNQLMVVASSPDTAREFCKT 85

Query: 185 NNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIRT 364
           ++R+FSGR LP VY+N+PGT+ +S+V++ EC+ +WK LR IGQNCIFSSKA+E+ A IR 
Sbjct: 86  HDRVFSGRHLPKVYHNLPGTLDSSIVLSPECSKSWKLLRSIGQNCIFSSKAMETNAWIRK 145

Query: 365 GQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFLN 544
            +V +MV  L+A ++G+VV++ED++FAT  NIIS+ + SRNLF+  G+  +D KVM  L 
Sbjct: 146 EKVMDMVNHLQA-REGQVVHLEDIMFATLANIISSALTSRNLFNIEGQETNDYKVMMALA 204

Query: 545 EEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERSTAI---NDNST 715
            E+I+K +  GL D  P LK +DFWSKG+AMDM+R I   WGDII ER T+    N NST
Sbjct: 205 NELIEKTTNLGLVDFLPSLKVIDFWSKGKAMDMHRKIRLTWGDIINERRTSAKHNNPNST 264

Query: 716 KDLLDVLNGHG-FPVDHIAILFTELLIAGTDSTTIT 820
           KD LD++   G +  D I+    ELLI  TDSTTIT
Sbjct: 265 KDFLDIVIADGTYSEDQISNALMELLIGATDSTTIT 300


>gb|PIN05630.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  290 bits (743), Expect = 2e-92
 Identities = 147/277 (53%), Positives = 198/277 (71%), Gaps = 4/277 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           +LPPGPN+  +   +  LI  PH+AL  LAKIYGPL+S  LG Q++VVAS+PD A+E  K
Sbjct: 25  QLPPGPNVLHVLGYLPQLIYKPHIALQKLAKIYGPLLSFHLGIQLMVVASSPDTAREFCK 84

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T++R+FSGR LP+VY+N+PGT+ +S+V++ EC+ +WK LR IGQNC+FSSKA+E+ A IR
Sbjct: 85  THDRVFSGRHLPTVYHNLPGTLDSSIVLSPECSKSWKLLRSIGQNCVFSSKAMETNARIR 144

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V +M+  LRA ++G+VV++ED++FAT  NIIS+ + SRNLFD  G+  +D K M  L
Sbjct: 145 KAKVMDMLTLLRA-REGQVVHLEDVMFATLANIISSALTSRNLFDIEGQETNDYKAMMAL 203

Query: 542 NEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERSTAI---NDNS 712
             E+I+K +T GL D  P+LK +DFWSK +AMDM R I   WGDI+ ER T+    N +S
Sbjct: 204 VNEVIEKTTTLGLVDFLPILKVIDFWSKRKAMDMCRKIKLTWGDIVNERRTSAKHDNPSS 263

Query: 713 TKDLLDVLNGHG-FPVDHIAILFTELLIAGTDSTTIT 820
           TKD LD++   G F  D I     ELLI  TDSTTIT
Sbjct: 264 TKDFLDIVIADGAFSEDQIGNALMELLIGATDSTTIT 300


>gb|PIM98211.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 493

 Score =  287 bits (735), Expect = 4e-91
 Identities = 149/277 (53%), Positives = 196/277 (70%), Gaps = 4/277 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           +LPPGPN+ ++   +S LI  PH+AL  LAKIYGPL+S RLG QI+VVAS+PDIA+E  K
Sbjct: 25  QLPPGPNVLKVLWYLSRLIYEPHIALQKLAKIYGPLLSFRLGNQIMVVASSPDIAREFCK 84

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T++R+FSGR LP VY N+PGT+ +S+V++ EC  +WK LR IGQNC+FSSKA+E+ A IR
Sbjct: 85  THDRVFSGRHLPIVYRNLPGTLDSSVVLSPECGKSWKLLRSIGQNCVFSSKAMETNAWIR 144

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V +MV  +RA + G+VV++ED++FAT  NIIS+ + SRNLFD   +   D K M  L
Sbjct: 145 KEKVMDMVNHIRA-RGGQVVHLEDIMFATLANIISSALTSRNLFDIERQEMDDYKQMMAL 203

Query: 542 NEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERSTA---INDNS 712
             E+I+K +T G  D  P+LKG+DFWSKG+AM+MYR I   WG II ER  +    N  S
Sbjct: 204 VNEVIEKTTTLGSVDFLPILKGIDFWSKGKAMNMYRKIKLTWGHIINERRISNGHDNPRS 263

Query: 713 TKDLLDVLNGHG-FPVDHIAILFTELLIAGTDSTTIT 820
           T+D LD++   G F  D I  +  ELLI  TDSTTIT
Sbjct: 264 TQDFLDIVLADGTFSEDQIGNVLMELLIGATDSTTIT 300


>ref|XP_012846401.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
 gb|EYU45168.1| hypothetical protein MIMGU_mgv1a023853mg [Erythranthe guttata]
          Length = 493

 Score =  286 bits (731), Expect = 1e-90
 Identities = 146/280 (52%), Positives = 196/280 (70%), Gaps = 7/280 (2%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           +LPPGPN F + + +S LI  PH+AL  LAK YGPLMS +LG Q++VVAS+P+ AKE  +
Sbjct: 25  QLPPGPNQFHVMSYLSQLIYKPHIALQTLAKAYGPLMSFQLGDQLMVVASSPETAKEFCR 84

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T++RIFSGR LPSVY+ +PGT+ +S+V++ EC   WK LR IGQ+C+FSS+A+E+   IR
Sbjct: 85  THDRIFSGRHLPSVYHILPGTIDSSIVLSPECGKAWKLLRTIGQSCVFSSRAMETNTWIR 144

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V + V  LR  + G+VVN+EDL+FAT  NIIS+ + ++NLF   GE + D K M  L
Sbjct: 145 KAKVMDTVNHLRTKQLGQVVNLEDLMFATLANIISSALTAKNLFHIEGEGKEDYKEMMSL 204

Query: 542 NEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERSTAINDN---- 709
             E+I+K +TFGL D  P+LKGVDFWS G+AMDMYR I   WG II+ER  +  ++    
Sbjct: 205 VNEVIEKTTTFGLVDFLPILKGVDFWSNGKAMDMYRKIKLTWGGIIQERRASTKNDQNPS 264

Query: 710 --STKDLLDVLNGHG-FPVDHIAILFTELLIAGTDSTTIT 820
             +++D LD++   G F  D IAI   ELLI  TDSTTIT
Sbjct: 265 SKTSQDFLDIVLSDGTFSEDQIAISLMELLIGATDSTTIT 304


>ref|XP_012837735.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
 gb|EYU37231.1| hypothetical protein MIMGU_mgv1a005037mg [Erythranthe guttata]
          Length = 499

 Score =  273 bits (697), Expect = 2e-85
 Identities = 141/278 (50%), Positives = 206/278 (74%), Gaps = 5/278 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN +Q+  K+S     PH+A S+LAK+YGP++SLRLGGQ ++VAS+   AKEI K
Sbjct: 27  KLPPGPNTWQVLRKVSEFRKQPHIAFSNLAKVYGPIISLRLGGQFLIVASSAATAKEILK 86

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T +R FSGRFLP VYY +P    +S+VM++ECN TWKFLRG+ QN IFS+K++ES AG+R
Sbjct: 87  TQDRNFSGRFLPLVYYKIPSAEQSSVVMSKECNATWKFLRGMSQNFIFSNKSVESTAGLR 146

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESE-SDAKVMPF 538
             +V EMV++LR +K G++VN+++L+ +T +NIIS+++ SRN+FD   E E +D + M  
Sbjct: 147 KSKVLEMVDYLR-SKRGEIVNLDNLMNSTVSNIISDVLVSRNVFDITKEDETNDHENMRA 205

Query: 539 LNEEIIDK-ASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKE-RSTAIND-- 706
           L  EI+D  +S+ GL+D+FP ++ +DF S+ +AM++++ +   WGD++KE RS  ++D  
Sbjct: 206 LVNEIVDMVSSSVGLSDLFPFMRILDFCSRKKAMEIHKKLMHMWGDMVKERRSDRVHDVN 265

Query: 707 NSTKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
            S +D LDVL  +    D I ++FTELLIAGTDS++IT
Sbjct: 266 YSARDFLDVLIDNSLTDDQIYVVFTELLIAGTDSSSIT 303


>ref|XP_011101194.2| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 369

 Score =  251 bits (642), Expect = 1e-78
 Identities = 135/273 (49%), Positives = 184/273 (67%), Gaps = 1/273 (0%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGPN +Q+   +S   N  HVA   LA+ +GPL SLRLG Q++VVAS+P IA +I KT
Sbjct: 30  LPPGPNPWQLLWTMSQSRNKTHVAFQTLARAHGPLFSLRLGSQLIVVASSPAIAMQILKT 89

Query: 185 NNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIRT 364
           +++IFSGR+LPS  +   G + +SL M+R+CNDTWKFLR IG N IFSS+A+ +   +R 
Sbjct: 90  HDKIFSGRYLPSRCFQFVGMVQSSLTMSRQCNDTWKFLRSIGHNFIFSSQAVGAKEKMRK 149

Query: 365 GQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFLN 544
             V EM+E+L  N +GK V ++D+V  T +N+++N++AS +LFD  GE E++ K +  L 
Sbjct: 150 EVVVEMMEYLVEN-EGKAVKLDDIVKVTVSNVVANVLASTSLFDVRGEGENEEK-LTGLV 207

Query: 545 EEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKE-RSTAINDNSTKD 721
            EI++ A+  GL D+FP L+ VDFWS+   M M   I   W DI++E R     D S +D
Sbjct: 208 REIVESAANLGLADLFPALRRVDFWSRRNGMKMREKIMCVWADIVEERRKCGTKDVSCRD 267

Query: 722 LLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
            LDVL  +GF  D I+IL  ELLIAGTDST IT
Sbjct: 268 FLDVLLENGFLDDQISILIMELLIAGTDSTIIT 300


>gb|EYU25066.1| hypothetical protein MIMGU_mgv1a021633mg, partial [Erythranthe
           guttata]
          Length = 471

 Score =  239 bits (610), Expect = 1e-72
 Identities = 131/280 (46%), Positives = 185/280 (66%), Gaps = 7/280 (2%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN  Q+  K+S   N PH+A++ LAK+YGP+MS  LG Q+V++AS+   AK+I K
Sbjct: 1   KLPPGPNALQLLRKVSEFRNKPHIAITTLAKLYGPIMSFHLGTQVVIIASSSSAAKQIFK 60

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T+++ FSGR+L SV+  +P   H SL M+RECN TWK LR + QN IFSSK++ S A  R
Sbjct: 61  THDQNFSGRYLTSVFCKLPTIEHCSLAMSRECNATWKLLRDVTQNVIFSSKSVHSKACTR 120

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V EMVE++R +K G+ V++EDL+ +T  NIIS+++ SRNL D   E E D KV   +
Sbjct: 121 KAKVAEMVEYIR-SKAGETVDLEDLMNSTIYNIISDVLVSRNLVDITREDEGDGKVRAVV 179

Query: 542 NEEIIDKASTFGLTDVFPMLKGVDFWS-KGRAMDMYRNINSNWGDIIKERSTAIND---- 706
            E I   +S+ GL+D+FP+L+ ++  S K +AM+++  I   W DI+KER +   D    
Sbjct: 180 REIIDLVSSSVGLSDLFPVLRKLNLPSTKRKAMEIHGKIMYIWEDIVKERRSEKRDRGLV 239

Query: 707 --NSTKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
             NS  D LDV+  +GFP D I  +  ELL AG D++ IT
Sbjct: 240 DANSGGDFLDVMIENGFPDDQICNVTMELLAAGMDTSGIT 279


>ref|XP_012852332.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
          Length = 501

 Score =  239 bits (610), Expect = 2e-72
 Identities = 131/280 (46%), Positives = 185/280 (66%), Gaps = 7/280 (2%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN  Q+  K+S   N PH+A++ LAK+YGP+MS  LG Q+V++AS+   AK+I K
Sbjct: 31  KLPPGPNALQLLRKVSEFRNKPHIAITTLAKLYGPIMSFHLGTQVVIIASSSSAAKQIFK 90

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T+++ FSGR+L SV+  +P   H SL M+RECN TWK LR + QN IFSSK++ S A  R
Sbjct: 91  THDQNFSGRYLTSVFCKLPTIEHCSLAMSRECNATWKLLRDVTQNVIFSSKSVHSKACTR 150

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V EMVE++R +K G+ V++EDL+ +T  NIIS+++ SRNL D   E E D KV   +
Sbjct: 151 KAKVAEMVEYIR-SKAGETVDLEDLMNSTIYNIISDVLVSRNLVDITREDEGDGKVRAVV 209

Query: 542 NEEIIDKASTFGLTDVFPMLKGVDFWS-KGRAMDMYRNINSNWGDIIKERSTAIND---- 706
            E I   +S+ GL+D+FP+L+ ++  S K +AM+++  I   W DI+KER +   D    
Sbjct: 210 REIIDLVSSSVGLSDLFPVLRKLNLPSTKRKAMEIHGKIMYIWEDIVKERRSEKRDRGLV 269

Query: 707 --NSTKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
             NS  D LDV+  +GFP D I  +  ELL AG D++ IT
Sbjct: 270 DANSGGDFLDVMIENGFPDDQICNVTMELLAAGMDTSGIT 309


>ref|XP_011083904.2| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 423

 Score =  233 bits (593), Expect = 1e-70
 Identities = 124/234 (52%), Positives = 164/234 (70%), Gaps = 6/234 (2%)
 Frame = +2

Query: 137 VVVASTPDIAKEIHKTNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQN 316
           +VVAS+PD AKE  KT++R+FSGR LPSVY+N+P TM +S+V++ EC  +WK LR IGQN
Sbjct: 1   MVVASSPDTAKEFCKTHDRMFSGRHLPSVYHNLPRTMDSSIVLSPECGRSWKLLRSIGQN 60

Query: 317 CIFSSKAIESAAGIRTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFD 496
           C+FSSKA+E+ A IR  +V EMV  + ++K+G+VVN+EDL+FAT  NIIS+++ SR+LFD
Sbjct: 61  CVFSSKAMETNAWIRKAKVMEMVSHI-SSKEGEVVNLEDLIFATLANIISSVLTSRDLFD 119

Query: 497 DGGESESDAKVMPFLNEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDI 676
              E++   +VM  L  E+I+K +T GL D  P+L+ VDFWSKG+AMDMYR I   WG I
Sbjct: 120 VKNEAKDKYRVMMGLVNEVIEKTTTLGLVDFLPVLRWVDFWSKGKAMDMYRKIKLTWGSI 179

Query: 677 IKER-----STAINDNSTKDLLD-VLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           I+ER     S   + +  +D LD VL    F  D I     ELLI  TDSTTIT
Sbjct: 180 IQERRSSKGSDHKSSSDGQDFLDIVLADSSFSEDQIGNALMELLIGATDSTTIT 233


>gb|EYU45167.1| hypothetical protein MIMGU_mgv1a024523mg [Erythranthe guttata]
          Length = 482

 Score =  228 bits (582), Expect = 2e-68
 Identities = 126/278 (45%), Positives = 185/278 (66%), Gaps = 5/278 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           +LPPGPN +Q+ + +S L++ PH                +LG Q++VVAS+PD AKE  +
Sbjct: 27  QLPPGPNQWQVLSYLSELMHKPH---------------FKLGNQLMVVASSPDYAKEFCR 71

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           T++ IFSGR LP+VY+ + GT+ +S+V++ +C  +WK LR I QNC+FSS+ +ES+A +R
Sbjct: 72  THDGIFSGRHLPAVYHYLQGTIGSSIVLSPDCGKSWKLLRSICQNCVFSSRVVESSAWMR 131

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLF-DDGGESESD-AKVMP 535
             +  E+++ LR  KDG+VVN+EDL+FAT  N+IS+++ SR+LF  +GG ++ D  +VM 
Sbjct: 132 KTKAKEILDHLR-GKDGEVVNLEDLMFATLANMISSVLTSRDLFVINGGAAKGDNYEVMM 190

Query: 536 FLNEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERST--AINDN 709
            +  E+I+  +  GL D  P+LKGVDFWSKG+A D+ R I   WGDII+ER +    N +
Sbjct: 191 DIVNEVIEGTTAPGLVDFLPILKGVDFWSKGKAKDICRKIKLTWGDIIQERKSFGRENPS 250

Query: 710 STKDLLD-VLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           S +D LD VL+   F  D I     E+LI  TD+TTIT
Sbjct: 251 SNRDFLDTVLSDTTFSDDQIGNALMEILIGATDTTTIT 288


>gb|EYU25065.1| hypothetical protein MIMGU_mgv1a019909mg, partial [Erythranthe
           guttata]
          Length = 482

 Score =  228 bits (580), Expect = 4e-68
 Identities = 129/281 (45%), Positives = 185/281 (65%), Gaps = 8/281 (2%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN +Q+  K+S   N PH+A++ LAK+YGP+MS  LG Q+VV+AS+   AK+I K
Sbjct: 11  KLPPGPNAWQLLRKVSEFRNKPHIAITSLAKLYGPIMSFHLGTQVVVIASSSSAAKQIFK 70

Query: 182 TNNRIFSGRFL-PSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGI 358
           T+++ FSGR+L  SV+  +P   H +L M+REC  TWK LR + Q+ IFSSK++ S A  
Sbjct: 71  THDQNFSGRYLMTSVFCKLPTIDHCTLAMSRECGATWKLLRDVTQSVIFSSKSVHSKACT 130

Query: 359 RTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPF 538
           R  +V EMVE +R +K G+ VN+E L+ +T +NIIS+++ SRNL D   ESESDA+V   
Sbjct: 131 RKAKVAEMVECIR-SKHGETVNLEVLMNSTISNIISDVLVSRNLVDITRESESDAEVRGV 189

Query: 539 LNEEIIDKASTFGLTDVFPMLKGVDFWS-KGRAMDMYRNINSNWGDIIKER------STA 697
           + E I   +S+ GL+D+FP+L+ ++  S K +AM+++  I   W DI+KER         
Sbjct: 190 VREIIELVSSSVGLSDLFPVLRRLNLPSTKRKAMEIHGKIMYIWEDIVKERRSEKRDGVL 249

Query: 698 INDNSTKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
            N NS  D LD +  +GFP D I  +  ELL AG D++ +T
Sbjct: 250 ANANSGGDFLDFMIENGFPDDQICNVTMELLAAGMDTSGMT 290


>ref|XP_012851911.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Erythranthe guttata]
          Length = 604

 Score =  228 bits (580), Expect = 7e-67
 Identities = 129/281 (45%), Positives = 185/281 (65%), Gaps = 8/281 (2%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN +Q+  K+S   N PH+A++ LAK+YGP+MS  LG Q+VV+AS+   AK+I K
Sbjct: 133 KLPPGPNAWQLLRKVSEFRNKPHIAITSLAKLYGPIMSFHLGTQVVVIASSSSAAKQIFK 192

Query: 182 TNNRIFSGRFL-PSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGI 358
           T+++ FSGR+L  SV+  +P   H +L M+REC  TWK LR + Q+ IFSSK++ S A  
Sbjct: 193 THDQNFSGRYLMTSVFCKLPTIDHCTLAMSRECGATWKLLRDVTQSVIFSSKSVHSKACT 252

Query: 359 RTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPF 538
           R  +V EMVE +R +K G+ VN+E L+ +T +NIIS+++ SRNL D   ESESDA+V   
Sbjct: 253 RKAKVAEMVECIR-SKHGETVNLEVLMNSTISNIISDVLVSRNLVDITRESESDAEVRGV 311

Query: 539 LNEEIIDKASTFGLTDVFPMLKGVDFWS-KGRAMDMYRNINSNWGDIIKER------STA 697
           + E I   +S+ GL+D+FP+L+ ++  S K +AM+++  I   W DI+KER         
Sbjct: 312 VREIIELVSSSVGLSDLFPVLRRLNLPSTKRKAMEIHGKIMYIWEDIVKERRSEKRDGVL 371

Query: 698 INDNSTKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
            N NS  D LD +  +GFP D I  +  ELL AG D++ +T
Sbjct: 372 ANANSGGDFLDFMIENGFPDDQICNVTMELLAAGMDTSGMT 412


>ref|XP_012846393.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Erythranthe guttata]
          Length = 426

 Score =  205 bits (522), Expect = 5e-60
 Identities = 112/233 (48%), Positives = 162/233 (69%), Gaps = 5/233 (2%)
 Frame = +2

Query: 137 VVVASTPDIAKEIHKTNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQN 316
           +VVAS+PD AKE  +T++ IFSGR LP+VY+ + GT+ +S+V++ +C  +WK LR I QN
Sbjct: 1   MVVASSPDYAKEFCRTHDGIFSGRHLPAVYHYLQGTIGSSIVLSPDCGKSWKLLRSICQN 60

Query: 317 CIFSSKAIESAAGIRTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLF- 493
           C+FSS+ +ES+A +R  +  E+++ LR  KDG+VVN+EDL+FAT  N+IS+++ SR+LF 
Sbjct: 61  CVFSSRVVESSAWMRKTKAKEILDHLR-GKDGEVVNLEDLMFATLANMISSVLTSRDLFV 119

Query: 494 DDGGESESD-AKVMPFLNEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWG 670
            +GG ++ D  +VM  +  E+I+  +  GL D  P+LKGVDFWSKG+A D+ R I   WG
Sbjct: 120 INGGAAKGDNYEVMMDIVNEVIEGTTAPGLVDFLPILKGVDFWSKGKAKDICRKIKLTWG 179

Query: 671 DIIKERST--AINDNSTKDLLD-VLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           DII+ER +    N +S +D LD VL+   F  D I     E+LI  TD+TTIT
Sbjct: 180 DIIQERKSFGRENPSSNRDFLDTVLSDTTFSDDQIGNALMEILIGATDTTTIT 232


>ref|XP_011078628.1| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 493

 Score =  201 bits (510), Expect = 1e-57
 Identities = 108/274 (39%), Positives = 172/274 (62%), Gaps = 1/274 (0%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           +LPPGP+ +Q+   + ++  +P  +L++LA+IYGP+ SLR+G  ++VVAS+P+ A EI K
Sbjct: 38  RLPPGPSAWQL---LRNMFMSPLASLANLAQIYGPIFSLRVGALLIVVASSPETAMEILK 94

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
            ++ IF GR++PS+YY +P    +S++ A+ECND+WK +RG   N +FS+KAI+    +R
Sbjct: 95  AHDHIFCGRYIPSLYYKIPHMERSSVLGAKECNDSWKHIRGTCHNLVFSTKAIKLRNEVR 154

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVMPFL 541
             +V EM ++LRA K+GKVV + D+VFAT  N + ++M S ++ D   E +   K +   
Sbjct: 155 ARKVMEMADYLRA-KEGKVVRLRDIVFATLANSMCHMMVSTDIIDLERECKDGTKSLMGF 213

Query: 542 NEEIIDKASTFGLTDVFPMLKG-VDFWSKGRAMDMYRNINSNWGDIIKERSTAINDNSTK 718
            + ++    T  L D+FP L G + FW + +A  +++   S WG++I+ER  A      +
Sbjct: 214 IKGMLTILGTPSLVDLFPALDGLLGFWERRKATKLHQEFKSLWGEVIEERRQA--KCGRE 271

Query: 719 DLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           DLLD L   GF  D I  L  E+L AGT ++  T
Sbjct: 272 DLLDALIDDGFCNDDICALLMEMLAAGTFTSCAT 305


>ref|XP_020549758.1| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 326

 Score =  191 bits (484), Expect = 2e-55
 Identities = 107/270 (39%), Positives = 169/270 (62%), Gaps = 5/270 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           + PP    +Q+   +S    +P  +L++LA+IYGP+ SLR+G  ++VVAS+P+ AKEI K
Sbjct: 30  RFPPTAYAWQLVCNMSM---SPLASLANLAQIYGPIFSLRVGALLIVVASSPETAKEILK 86

Query: 182 TNNRIFSGRFLPSVYYN-VPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGI 358
             + IFSGR++PS+YY  +P    +S++ +RECND WK++RG+  N +FS+KAI+    I
Sbjct: 87  ARDHIFSGRYIPSLYYKIIPHMEQSSVLGSRECNDDWKYIRGMCHNWLFSNKAIKLRNEI 146

Query: 359 RTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESES-DAKVMP 535
           R  ++ EM ++LRA K+GK++ + ++VFAT  N +SN+M SR+L D G E E  + ++M 
Sbjct: 147 RERKMMEMADYLRA-KEGKIMTLRNIVFATLANSMSNMMVSRDLIDLGRECEGRNQRLMG 205

Query: 536 FLNEEIIDKASTFGLTDVFPMLKG-VDFWSKGRAMDMYRNINSNWGDIIKER--STAIND 706
           F+   ++D   T  + D+FP L+G + FW + +A  +++   S WG +I+ER        
Sbjct: 206 FIEAIMLDVLGTPSVVDLFPTLEGLLGFWERRKATKLHQEFKSLWGKVIEERREEKCAGK 265

Query: 707 NSTKDLLDVLNGHGFPVDHIAILFTELLIA 796
               DLLD L  +GF  D I  L    LI+
Sbjct: 266 APYHDLLDALIDNGFCNDKICALLMVNLIS 295


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  188 bits (478), Expect = 8e-53
 Identities = 118/277 (42%), Positives = 166/277 (59%), Gaps = 5/277 (1%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGP  + I   +  +   PH  L++LAK++GPLMSLRLG Q++VVAS+P  A E+ KT
Sbjct: 38  LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKT 97

Query: 185 NNRIFSGRFL-PSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           ++R  SGR+L  SV    P   H S+V A++CN  WK LR I +  +FS KA+ES   +R
Sbjct: 98  HDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELR 157

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNL--FDDGGESESDAKVMP 535
             +VTE+VEFL A K+G+VV + DLVF T  NI+SN   S +L  F+D G      K + 
Sbjct: 158 ERKVTELVEFL-ATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLI 216

Query: 536 FLNEEIIDKASTFGLTDVFPMLKGVDFWSKGR-AMDMYRNINSNWGDIIKERST-AINDN 709
             N E     +T  L+D +P+L G+D     R A +M+  I + W DI+KER T   N +
Sbjct: 217 HKNAEF---GATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRS 273

Query: 710 STKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           S +D L+ L   GF  D I  +  EL  AG +++++T
Sbjct: 274 SHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310


>ref|XP_011084765.1| probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Sesamum
           indicum]
          Length = 498

 Score =  188 bits (478), Expect = 8e-53
 Identities = 113/277 (40%), Positives = 169/277 (61%), Gaps = 4/277 (1%)
 Frame = +2

Query: 2   KLPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHK 181
           KLPPGPN +Q+   IS L N PHVA  +LAKIYGPL+S+RLG Q++VVAS+P  AK I K
Sbjct: 30  KLPPGPNAWQVLLNISQLRNKPHVAFHNLAKIYGPLISMRLGAQLLVVASSPATAKAILK 89

Query: 182 TNNRIFSGRFLPSVYYNVPGTMHASLV--MARECNDTWKFLRGIGQNCIFSSKAIES-AA 352
           T++R+FSGR+ PS+ Y  P T  +  +   ++EC+  +++      +   + + +ES   
Sbjct: 90  THDRVFSGRYSPSISYAFPTTSQSYTMRSTSKECSGMFEYASCSIMDLFSTLREVESKGK 149

Query: 353 GIRTGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNLFDDGGESESDAKVM 532
           G +   + EM+++L   K+G+VV ++D++ ATF NI +N++ASRNL   G E+    ++ 
Sbjct: 150 GRKEKVLMEMMDYL-VGKEGEVVKLDDVLKATFFNISANVLASRNLL--GYENWGKGEMA 206

Query: 533 PFLNEEIIDKASTFGLTDVFPMLKGVDFWSKGRAMDMYRNINSNWGDIIKERSTAIND-N 709
             L  EI +   T GL D+FP+L+ V FW K     + R +   W DI+K+R +     +
Sbjct: 207 KDLVNEIEEVFLTPGLLDLFPVLRRVYFWRKRDGKILRRKVMCLWEDILKQRRSRNGSID 266

Query: 710 STKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
             +D LDVL  + FP D I  +  ELLIAG DS  +T
Sbjct: 267 KGRDFLDVLLENAFPDDLICFVLMELLIAGGDSGVVT 303


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  188 bits (478), Expect = 8e-53
 Identities = 118/277 (42%), Positives = 166/277 (59%), Gaps = 5/277 (1%)
 Frame = +2

Query: 5   LPPGPNIFQISAKISSLINTPHVALSDLAKIYGPLMSLRLGGQIVVVASTPDIAKEIHKT 184
           LPPGP  + I   +  +   PH  L++LAK++GPLMSLRLG Q++VVAS+P  A E+ KT
Sbjct: 38  LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKT 97

Query: 185 NNRIFSGRFL-PSVYYNVPGTMHASLVMARECNDTWKFLRGIGQNCIFSSKAIESAAGIR 361
           ++R  SGR+L  SV    P   H S+V A++CN  WK LR I +  +FS KA+ES   +R
Sbjct: 98  HDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELR 157

Query: 362 TGQVTEMVEFLRANKDGKVVNIEDLVFATFTNIISNIMASRNL--FDDGGESESDAKVMP 535
             +VTE+VEFL A K+G+VV + DLVF T  NI+SN   S +L  F+D G      K + 
Sbjct: 158 ERKVTELVEFL-ATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLI 216

Query: 536 FLNEEIIDKASTFGLTDVFPMLKGVDFWSKGR-AMDMYRNINSNWGDIIKERST-AINDN 709
             N E     +T  L+D +P+L G+D     R A +M+  I + W DI+KER T   N +
Sbjct: 217 HKNAEF---GATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRS 273

Query: 710 STKDLLDVLNGHGFPVDHIAILFTELLIAGTDSTTIT 820
           S +D L+ L   GF  D I  +  EL  AG +++++T
Sbjct: 274 SHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLT 310