BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00006696
         (1869 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011087622.1| mitotic spindle checkpoint protein MAD1 [Ses...   725   0.0  
ref|XP_012840251.1| PREDICTED: mitotic spindle checkpoint protei...   680   0.0  
gb|EYU35035.1| hypothetical protein MIMGU_mgv1a002021mg [Erythra...   664   0.0  
ref|XP_022861405.1| mitotic spindle checkpoint protein MAD1 isof...   599   0.0  
ref|XP_022861406.1| mitotic spindle checkpoint protein MAD1 isof...   594   0.0  
emb|CDO97655.1| unnamed protein product [Coffea canephora]            557   0.0  
ref|XP_019169507.1| PREDICTED: mitotic spindle checkpoint protei...   542   0.0  
ref|XP_019169506.1| PREDICTED: mitotic spindle checkpoint protei...   542   0.0  
ref|XP_016500353.1| PREDICTED: mitotic spindle checkpoint protei...   537   0.0  
ref|XP_016500351.1| PREDICTED: mitotic spindle checkpoint protei...   537   0.0  
ref|XP_009791242.1| PREDICTED: early endosome antigen 1 isoform ...   533   e-180
ref|XP_016500350.1| PREDICTED: mitotic spindle checkpoint protei...   537   e-180
ref|XP_009627035.1| PREDICTED: mitotic spindle checkpoint protei...   537   e-180
ref|XP_006349279.1| PREDICTED: mitotic spindle checkpoint protei...   536   e-180
ref|XP_015060942.1| PREDICTED: mitotic spindle checkpoint protei...   534   e-179
ref|XP_019230208.1| PREDICTED: mitotic spindle checkpoint protei...   530   e-179
ref|XP_009791238.1| PREDICTED: spindle assembly checkpoint compo...   533   e-179
ref|XP_019230207.1| PREDICTED: mitotic spindle checkpoint protei...   530   e-177
ref|XP_016538948.1| PREDICTED: mitotic spindle checkpoint protei...   530   e-177
ref|XP_004230415.1| PREDICTED: mitotic spindle checkpoint protei...   529   e-177

>ref|XP_011087622.1| mitotic spindle checkpoint protein MAD1 [Sesamum indicum]
          Length = 728

 Score =  725 bits (1872), Expect = 0.0
 Identities = 382/518 (73%), Positives = 421/518 (81%), Gaps = 1/518 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPED-GNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPP RKRRA+SQPPED  NNN  SPSS+++LLVYEDLP PEYSHDHS PITSDQMLCTY
Sbjct: 4    RTPPPRKRRADSQPPEDINNNNAQSPSSNRELLVYEDLPVPEYSHDHSIPITSDQMLCTY 63

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EFFDALSSAEKQ HDYQSKF ALNED S AEAERK FR+KFFNAEQELAAAK
Sbjct: 64   QCRQMVKSEFFDALSSAEKQVHDYQSKFGALNEDLSNAEAERKKFREKFFNAEQELAAAK 123

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GREQMLQEQLRKE+DFSQEQLRKQI S+SELEVKF+ EMDLR                  
Sbjct: 124  GREQMLQEQLRKEVDFSQEQLRKQIHSFSELEVKFQKEMDLRQKAESSAAKAEEKASLLE 183

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L TLSESIDREKNRLQN+L+QMRSESKLSVSRI+ADLE+MEC+A NA           
Sbjct: 184  EKLSTLSESIDREKNRLQNELLQMRSESKLSVSRISADLERMECKATNAEKESALLREQL 243

Query: 848  XXXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKAS 1027
                     CMQEK+E+EKK S +SPE PS+D++LL+KHLQEELRNYESEVREARK+KAS
Sbjct: 244  EELKKRLDKCMQEKYELEKKSSIRSPEVPSQDSNLLLKHLQEELRNYESEVREARKLKAS 303

Query: 1028 HEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADD 1207
            HEDI+LLKEKLYEEK+RRER E           NVKRLE ELSTWKSVV+EIP VSSADD
Sbjct: 304  HEDIELLKEKLYEEKSRRERTELDLLKLSDLQVNVKRLEGELSTWKSVVREIPGVSSADD 363

Query: 1208 IIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAE 1387
            I+PKFAALQKEVVDSMM+AS+AQ CMKEIQ SLD+A+LDKQNAETEA+MAK++AESYKAE
Sbjct: 364  ILPKFAALQKEVVDSMMRASDAQACMKEIQVSLDAAILDKQNAETEAAMAKERAESYKAE 423

Query: 1388 IKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXX 1567
             K+LE +LGLITEERDRLKNV++ L+E T+ E GTE  SGTIVQELEASLAKKENYV   
Sbjct: 424  NKQLESVLGLITEERDRLKNVLKLLKERTSLEGGTESVSGTIVQELEASLAKKENYVTEL 483

Query: 1568 XXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                     INSRQ NEIMLLNERL NEARRVKMLERE
Sbjct: 484  ENSLLEKKEINSRQQNEIMLLNERLANEARRVKMLERE 521



 Score =  117 bits (294), Expect = 8e-24
 Identities = 59/64 (92%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSS NTKVLRMVNTLAVDNEAKQTI+ALQNELQK KEKLQAVEELKKQS+DA
Sbjct: 533  ESKLGHGDFSSTNTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEKLQAVEELKKQSSDA 592

Query: 1857 GTHV 1868
            GTHV
Sbjct: 593  GTHV 596


>ref|XP_012840251.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Erythranthe
            guttata]
          Length = 731

 Score =  680 bits (1754), Expect = 0.0
 Identities = 367/521 (70%), Positives = 408/521 (78%), Gaps = 4/521 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPED---GNNNTHSPSSSKQ-LLVYEDLPAPEYSHDHSTPITSDQML 298
            RTP  RKRRAES+PPED    NNNT +P+S++Q LL+YEDL APEYSH HSTP TSDQML
Sbjct: 4    RTPAPRKRRAESEPPEDDNNNNNNTQTPNSNRQQLLIYEDLQAPEYSHGHSTPTTSDQML 63

Query: 299  CTYQCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELA 478
            CTYQCRQMVK+EFFDALSSAEKQAHDYQSKF+ALNEDFSK+E+ERK FRDK FNAEQEL+
Sbjct: 64   CTYQCRQMVKSEFFDALSSAEKQAHDYQSKFKALNEDFSKSESERKKFRDKCFNAEQELS 123

Query: 479  AAKGREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXX 658
            AAKGREQ LQEQLRKEIDFSQE LRKQIQS+SELEVKF+ EMDLR               
Sbjct: 124  AAKGREQNLQEQLRKEIDFSQEHLRKQIQSFSELEVKFQKEMDLRKKAELTAAKAEDKAS 183

Query: 659  XXXXNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXX 838
                 L T+SESI REK+RLQN+LVQMRSESKLS+SRI+ADLEKME RAKNA        
Sbjct: 184  VLEEKLSTVSESIVREKSRLQNELVQMRSESKLSLSRISADLEKMEFRAKNAEKESALLK 243

Query: 839  XXXXXXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKI 1018
                         M+EK+E++K +S KSPE  SKD +LLVKHLQEELRNYESEVREARKI
Sbjct: 244  EQLEENKRRMDEFMKEKYEMDKNVSVKSPEASSKDANLLVKHLQEELRNYESEVREARKI 303

Query: 1019 KASHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSS 1198
            KA HEDI+LLKEKLYEEK RRERAE           NV  L DELS WKSVVKEIP V S
Sbjct: 304  KAYHEDIELLKEKLYEEKRRRERAELDLVKLSDLQLNVNTLNDELSIWKSVVKEIPGVLS 363

Query: 1199 ADDIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESY 1378
            ADDI  KFAALQ+EV DSM +A EAQ CMKEIQ +LD+A+LDKQ AETEA++AK+KAES 
Sbjct: 364  ADDIPSKFAALQREVADSMRRAGEAQACMKEIQVALDTAMLDKQTAETEAALAKEKAESS 423

Query: 1379 KAEIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYV 1558
            +AEIKRLELM GLITEERDRL+N+V+EL+E  N E GTEL  GTIVQELE+SLAKKENYV
Sbjct: 424  EAEIKRLELMFGLITEERDRLQNLVKELKERKNVEGGTELAGGTIVQELESSLAKKENYV 483

Query: 1559 XXXXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                        INSRQHNEI LLNERLTNEARR+KMLER+
Sbjct: 484  RELENSLSEQKEINSRQHNEIKLLNERLTNEARRLKMLERD 524



 Score =  114 bits (286), Expect = 8e-23
 Identities = 57/64 (89%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSSANTKVLRMVNTLAVDNEAKQTI+ALQNELQK KE+LQAV+ELKKQS+DA
Sbjct: 536  ESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEQLQAVDELKKQSSDA 595

Query: 1857 GTHV 1868
            G HV
Sbjct: 596  GAHV 599


>gb|EYU35035.1| hypothetical protein MIMGU_mgv1a002021mg [Erythranthe guttata]
          Length = 725

 Score =  664 bits (1713), Expect = 0.0
 Identities = 362/521 (69%), Positives = 403/521 (77%), Gaps = 4/521 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPED---GNNNTHSPSSSKQ-LLVYEDLPAPEYSHDHSTPITSDQML 298
            RTP  RKRRAES+PPED    NNNT +P+S++Q LL+YEDL APEYSH HSTP TSDQML
Sbjct: 4    RTPAPRKRRAESEPPEDDNNNNNNTQTPNSNRQQLLIYEDLQAPEYSHGHSTPTTSDQML 63

Query: 299  CTYQCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELA 478
            CTYQCRQMVK+EFFDALSSAEKQAHDYQSKF+ALNEDFSK+E+ERK FRDK FNAEQEL+
Sbjct: 64   CTYQCRQMVKSEFFDALSSAEKQAHDYQSKFKALNEDFSKSESERKKFRDKCFNAEQELS 123

Query: 479  AAKGREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXX 658
            AAKGREQ LQEQLRKEIDFSQE LRKQIQS+SELEVKF+ EMDLR               
Sbjct: 124  AAKGREQNLQEQLRKEIDFSQEHLRKQIQSFSELEVKFQKEMDLRKKAELTAAKAEDKAS 183

Query: 659  XXXXNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXX 838
                 L T+SESI REK+RLQN+LVQMRSESKLS+SRI+ADLEKME RAKNA        
Sbjct: 184  VLEEKLSTVSESIVREKSRLQNELVQMRSESKLSLSRISADLEKMEFRAKNAEKESALLK 243

Query: 839  XXXXXXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKI 1018
                         M+EK+E++K +S KSPE  SKD +LLVKHLQEELRNYESEVREARKI
Sbjct: 244  EQLEENKRRMDEFMKEKYEMDKNVSVKSPEASSKDANLLVKHLQEELRNYESEVREARKI 303

Query: 1019 KASHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSS 1198
            KA HEDI+LLKEKLYEEK RRERAE           NV  L DELS WKSVVKEIP V S
Sbjct: 304  KAYHEDIELLKEKLYEEKRRRERAELDLVKLSDLQLNVNTLNDELSIWKSVVKEIPGVLS 363

Query: 1199 ADDIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESY 1378
            ADDI  KFAALQ+EV DSM +A EAQ CMKEIQ +LD+A+LDKQ AETEA++AK+KAES 
Sbjct: 364  ADDIPSKFAALQREVADSMRRAGEAQACMKEIQVALDTAMLDKQTAETEAALAKEKAESS 423

Query: 1379 KAEIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYV 1558
            +AEIKRLELM      ERDRL+N+V+EL+E  N E GTEL  GTIVQELE+SLAKKENYV
Sbjct: 424  EAEIKRLELM------ERDRLQNLVKELKERKNVEGGTELAGGTIVQELESSLAKKENYV 477

Query: 1559 XXXXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                        INSRQHNEI LLNERLTNEARR+KMLER+
Sbjct: 478  RELENSLSEQKEINSRQHNEIKLLNERLTNEARRLKMLERD 518



 Score =  114 bits (286), Expect = 8e-23
 Identities = 57/64 (89%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSSANTKVLRMVNTLAVDNEAKQTI+ALQNELQK KE+LQAV+ELKKQS+DA
Sbjct: 530  ESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEQLQAVDELKKQSSDA 589

Query: 1857 GTHV 1868
            G HV
Sbjct: 590  GAHV 593


>ref|XP_022861405.1| mitotic spindle checkpoint protein MAD1 isoform X1 [Olea europaea
            var. sylvestris]
          Length = 727

 Score =  599 bits (1545), Expect = 0.0
 Identities = 322/520 (61%), Positives = 386/520 (74%), Gaps = 2/520 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQ-LLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPPARKRRAES+PPED  N+T+ PSS++Q L++YED P  E SHDHSTPIT+DQMLCTY
Sbjct: 4    RTPPARKRRAESRPPED--NDTNIPSSNRQQLVIYEDHPVVETSHDHSTPITTDQMLCTY 61

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EF DALSSAEKQAHDYQSK EAL+ DF KAEAERK FRD+FFNAEQELAAAK
Sbjct: 62   QCRQMVKSEFLDALSSAEKQAHDYQSKLEALSGDFYKAEAERKKFRDQFFNAEQELAAAK 121

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GREQMLQ+QLRKE+DFSQEQ++KQ+QSYSELE+K R EMDLR                  
Sbjct: 122  GREQMLQQQLRKEVDFSQEQIKKQMQSYSELEIKLRTEMDLRKKAESSAAIAEEKASGLE 181

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L TLSES++REK+R +N+L QMRS SKLS++RI+ADLE+MEC+AKNA           
Sbjct: 182  GKLNTLSESMEREKSRFENELAQMRSGSKLSITRISADLERMECKAKNAEKESALLKQQL 241

Query: 848  XXXXXXXXXCMQEKFEVEKKLSA-KSPETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CM EKFEVEK+ S   S ETPSKD  LLVKHLQEELR+YE EVREARKI +
Sbjct: 242  EELKKRLHECMGEKFEVEKRFSRFTSLETPSKDADLLVKHLQEELRSYECEVREARKIIS 301

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE I LLKE+L EEK RRER E            VK LED+LSTWKS++K+IP VS AD
Sbjct: 302  SHEKIDLLKEQLLEEKGRRERTESDLHNLADIQLTVKNLEDDLSTWKSMIKDIPGVSHAD 361

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            DI  KFAALQKE+ DSMMK  EAQ  +K ++ +LD+ +LDK++AETEA++AK++ E  K+
Sbjct: 362  DIPLKFAALQKELYDSMMKVGEAQARLKRVEVALDTTLLDKESAETEAALAKERMELSKS 421

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKRL+LM+  +TEERD LK+ V E++   + +   EL SGTIVQ+LE+SLAKKE+ +  
Sbjct: 422  EIKRLKLMIASLTEERDSLKHAVDEMKGCNSLDDRKELVSGTIVQQLESSLAKKESVIRE 481

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLEREE 1684
                       N  Q +EI LLNERL NEARR+KMLERE+
Sbjct: 482  LENNLSEQRETNRCQLDEIKLLNERLNNEARRIKMLERED 521



 Score =  112 bits (280), Expect = 4e-22
 Identities = 57/64 (89%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSSANTKVLRMVNTLAV+NEAKQTI+ALQNELQK KEKLQAVE+LKKQS+DA
Sbjct: 532  ESKLGHGDFSSANTKVLRMVNTLAVENEAKQTIEALQNELQKTKEKLQAVEDLKKQSSDA 591

Query: 1857 GTHV 1868
            GT V
Sbjct: 592  GTLV 595


>ref|XP_022861406.1| mitotic spindle checkpoint protein MAD1 isoform X2 [Olea europaea
            var. sylvestris]
          Length = 726

 Score =  594 bits (1531), Expect = 0.0
 Identities = 322/520 (61%), Positives = 385/520 (74%), Gaps = 2/520 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQ-LLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPPARKRRAES+PPED  N+T+ PSS++Q L++YED P  E SHDHSTPIT+DQMLCTY
Sbjct: 4    RTPPARKRRAESRPPED--NDTNIPSSNRQQLVIYEDHPVVETSHDHSTPITTDQMLCTY 61

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EF DALSSAEKQAHDYQSK EAL+ DF KAEAERK FRD+FFNAEQELAAAK
Sbjct: 62   QCRQMVKSEFLDALSSAEKQAHDYQSKLEALSGDFYKAEAERKKFRDQFFNAEQELAAAK 121

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GREQMLQ+QLRKE+DFSQEQ++KQ+QSYSELE+K R EMDLR                  
Sbjct: 122  GREQMLQQQLRKEVDFSQEQIKKQMQSYSELEIKLRTEMDLRKKAESSAAIAEEKASGLE 181

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L TLSES++REK+R +N+L QMRS SKLS++RI+ADLE+MEC+AKNA           
Sbjct: 182  GKLNTLSESMEREKSRFENELAQMRSGSKLSITRISADLERMECKAKNAEKESALLKQQL 241

Query: 848  XXXXXXXXXCMQEKFEVEKKLSA-KSPETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CM EKFEVEK+ S   S ETPSKD  LLVKHLQEELR+YE EVREARKI +
Sbjct: 242  EELKKRLHECMGEKFEVEKRFSRFTSLETPSKDADLLVKHLQEELRSYECEVREARKIIS 301

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE I LLKE+L EEK RRER E            VK LED+LSTWKS++K+IP VS AD
Sbjct: 302  SHEKIDLLKEQLLEEKGRRERTESDLHNLADIQLTVKNLEDDLSTWKSMIKDIPGVSHAD 361

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            DI  KFAALQKE+ DSMMK  EAQ  +K ++ +LD+ +LDK++AETEA++AK++ E  K+
Sbjct: 362  DIPLKFAALQKELYDSMMKVGEAQARLKRVEVALDTTLLDKESAETEAALAKERMELSKS 421

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKRL+LM+  +TEERD LK+ V E++   + +   EL SGTIVQ LE+SLAKKE+ +  
Sbjct: 422  EIKRLKLMIASLTEERDSLKHAVDEMKGCNSLDDRKELVSGTIVQ-LESSLAKKESVIRE 480

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLEREE 1684
                       N  Q +EI LLNERL NEARR+KMLERE+
Sbjct: 481  LENNLSEQRETNRCQLDEIKLLNERLNNEARRIKMLERED 520



 Score =  112 bits (280), Expect = 4e-22
 Identities = 57/64 (89%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSSANTKVLRMVNTLAV+NEAKQTI+ALQNELQK KEKLQAVE+LKKQS+DA
Sbjct: 531  ESKLGHGDFSSANTKVLRMVNTLAVENEAKQTIEALQNELQKTKEKLQAVEDLKKQSSDA 590

Query: 1857 GTHV 1868
            GT V
Sbjct: 591  GTLV 594


>emb|CDO97655.1| unnamed protein product [Coffea canephora]
          Length = 728

 Score =  557 bits (1436), Expect = 0.0
 Identities = 302/522 (57%), Positives = 381/522 (72%), Gaps = 5/522 (0%)
 Frame = +2

Query: 131  RTPPARKRRAES-QPPEDGNNNTHSPSSSKQLLVYEDLPA---PEYSHDHSTPITSDQML 298
            RTP  RKRRAES + P +  NN  +  S+KQL++YED P+   PE SHDH  PITSD ML
Sbjct: 4    RTPAPRKRRAESARSPNNTKNNVSN--SNKQLVIYEDSPSAPVPESSHDH--PITSDHML 59

Query: 299  CTYQCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELA 478
            CTYQCRQMVKAEFFDALS+AEKQA + QSK +ALNEDF KAE ERK FRDK  +AEQELA
Sbjct: 60   CTYQCRQMVKAEFFDALSNAEKQARECQSKLDALNEDFCKAETERKRFRDKLLSAEQELA 119

Query: 479  AAKGREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXX 658
            AAK REQ LQEQL K ++ S+E+LR+Q+Q ++ELE+KF+NE++LR               
Sbjct: 120  AAKRREQDLQEQLMKGVNSSEERLRRQLQLFNELEIKFQNEVNLREKAQSLAALAEEKAS 179

Query: 659  XXXXNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXX 838
                 L+ +S+S ++EK RLQN+L  ++S+SKLSVSRI+ADLE+MECRAK+A        
Sbjct: 180  ALEAKLQKVSQSTEKEKIRLQNELAHLKSDSKLSVSRISADLERMECRAKHAEEESKLLK 239

Query: 839  XXXXXXXXXXXXCMQEKFEVEKKLSAKSP-ETPSKDTSLLVKHLQEELRNYESEVREARK 1015
                        CMQ+K E+EK+L   +P E+PS D+S+L+KHLQEELRNYESEVREARK
Sbjct: 240  DQMEQLGKRLHECMQQKGEMEKRLLHATPQESPSTDSSILLKHLQEELRNYESEVREARK 299

Query: 1016 IKASHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVS 1195
            +K+SHE+++LLKEKL EEK RRERAE            +++L+DELS WKS++K+IP VS
Sbjct: 300  LKSSHENVELLKEKLQEEKRRRERAELDLLKLPEIQLRIEKLQDELSAWKSLMKDIPGVS 359

Query: 1196 SADDIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAES 1375
             ADD+  KFAALQKEV+DSMMK  EAQV +KEI+ +LD+A LDKQNA TEA++AK+KAES
Sbjct: 360  CADDVPMKFAALQKEVIDSMMKVGEAQVRLKEIEVALDAAELDKQNAITEATLAKEKAES 419

Query: 1376 YKAEIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENY 1555
             K+E+KR+ELML    EE+DRLKN+  EL+   + + G E+ SG +VQELEAS+AKKENY
Sbjct: 420  SKSEVKRIELMLASALEEKDRLKNMFNELKREKHAQGGPEVASGALVQELEASIAKKENY 479

Query: 1556 VXXXXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
            +            IN  Q NEI LL+ERLT+EARR+K LERE
Sbjct: 480  IKELESNLHEQNLINIHQQNEIKLLSERLTDEARRIKSLERE 521



 Score =  116 bits (290), Expect = 3e-23
 Identities = 60/64 (93%), Positives = 61/64 (95%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGDFSSANTKVLRMVNTLAVDNEAKQTI+ALQNELQK KEKLQAVEELKKQSADA
Sbjct: 533  ESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEKLQAVEELKKQSADA 592

Query: 1857 GTHV 1868
            GT V
Sbjct: 593  GTLV 596


>ref|XP_019169507.1| PREDICTED: mitotic spindle checkpoint protein MAD1 isoform X2
            [Ipomoea nil]
          Length = 622

 Score =  542 bits (1397), Expect = 0.0
 Identities = 297/520 (57%), Positives = 373/520 (71%), Gaps = 3/520 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQ-LLVYEDLP-APEYSHDHSTPITSDQMLCT 304
            RTPP RKRRAES+      N   SP S++Q L++YED P APE SHDH  P TS+QMLCT
Sbjct: 4    RTPPLRKRRAESEAKPVEEN---SPISNRQQLVIYEDDPSAPESSHDH--PFTSEQMLCT 58

Query: 305  YQCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAA 484
            YQCRQMVK+EF DALSSAEKQA DYQSKFEALN++  KA+AERK FRD+F  AEQELAAA
Sbjct: 59   YQCRQMVKSEFLDALSSAEKQAQDYQSKFEALNDELRKADAERKKFRDQFLYAEQELAAA 118

Query: 485  KGREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXX 664
            K RE+ LQEQL KE+D S+E+LRKQ+Q Y+ELEVKF++E +LR                 
Sbjct: 119  KRREESLQEQLLKEVDSSEERLRKQLQLYNELEVKFQHETNLRAKAESAASAAEEKASRC 178

Query: 665  XXNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXX 844
                 +LSESI+REK+RL N+L Q++SESK SV+RI+ + E+MECRA NA          
Sbjct: 179  EREFSSLSESIEREKSRLHNELEQLKSESKTSVTRISKNFERMECRAVNAEKESVLLKEQ 238

Query: 845  XXXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIK 1021
                      CM++K E+EKKLS+ + PE  S D ++LVKHLQEELRNYE+EVREARK+K
Sbjct: 239  MEELRVRLKECMEQKSEMEKKLSSITFPEGSSADNNILVKHLQEELRNYEAEVREARKLK 298

Query: 1022 ASHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSA 1201
            +SHE+I+LLKEKL EEK RR+RAE            VK+LEDELSTWKS+V EIP VS A
Sbjct: 299  SSHENIELLKEKLLEEKGRRDRAEAELLKLADIQLTVKKLEDELSTWKSMVLEIPGVSCA 358

Query: 1202 DDIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYK 1381
            DDI  KFA+LQKEV+DSM+K  EAQ  +K+++ +LD+A LDK N E E ++AK+KAE  K
Sbjct: 359  DDIPRKFASLQKEVLDSMIKMGEAQASLKQMEVALDNAKLDKSNTEAEVNLAKEKAEILK 418

Query: 1382 AEIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVX 1561
            +E+KR ELML  ++EERDRLK +V+E R   + ++G E+  G +VQELE SLAKKEN++ 
Sbjct: 419  SELKRTELMLASVSEERDRLKYIVEEPRTQKSMDTGIEVSGGKVVQELELSLAKKENFIK 478

Query: 1562 XXXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                       +N RQ NE+ LLN+RL++EARR+K LERE
Sbjct: 479  ELESSLSEQKEVNGRQLNELKLLNDRLSSEARRIKSLERE 518



 Score =  102 bits (254), Expect = 5e-19
 Identities = 55/67 (82%), Positives = 57/67 (85%), Gaps = 3/67 (4%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQS--- 1847
            E KLGHGD+SSA TKVLRMVNTL VDNE KQTI+ALQNELQK KEKL AVEELKKQS   
Sbjct: 530  ESKLGHGDYSSATTKVLRMVNTLGVDNETKQTIEALQNELQKTKEKLHAVEELKKQSDYP 589

Query: 1848 ADAGTHV 1868
            ADAGT V
Sbjct: 590  ADAGTLV 596


>ref|XP_019169506.1| PREDICTED: mitotic spindle checkpoint protein MAD1 isoform X1
            [Ipomoea nil]
          Length = 728

 Score =  542 bits (1397), Expect = 0.0
 Identities = 297/520 (57%), Positives = 373/520 (71%), Gaps = 3/520 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQ-LLVYEDLP-APEYSHDHSTPITSDQMLCT 304
            RTPP RKRRAES+      N   SP S++Q L++YED P APE SHDH  P TS+QMLCT
Sbjct: 4    RTPPLRKRRAESEAKPVEEN---SPISNRQQLVIYEDDPSAPESSHDH--PFTSEQMLCT 58

Query: 305  YQCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAA 484
            YQCRQMVK+EF DALSSAEKQA DYQSKFEALN++  KA+AERK FRD+F  AEQELAAA
Sbjct: 59   YQCRQMVKSEFLDALSSAEKQAQDYQSKFEALNDELRKADAERKKFRDQFLYAEQELAAA 118

Query: 485  KGREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXX 664
            K RE+ LQEQL KE+D S+E+LRKQ+Q Y+ELEVKF++E +LR                 
Sbjct: 119  KRREESLQEQLLKEVDSSEERLRKQLQLYNELEVKFQHETNLRAKAESAASAAEEKASRC 178

Query: 665  XXNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXX 844
                 +LSESI+REK+RL N+L Q++SESK SV+RI+ + E+MECRA NA          
Sbjct: 179  EREFSSLSESIEREKSRLHNELEQLKSESKTSVTRISKNFERMECRAVNAEKESVLLKEQ 238

Query: 845  XXXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIK 1021
                      CM++K E+EKKLS+ + PE  S D ++LVKHLQEELRNYE+EVREARK+K
Sbjct: 239  MEELRVRLKECMEQKSEMEKKLSSITFPEGSSADNNILVKHLQEELRNYEAEVREARKLK 298

Query: 1022 ASHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSA 1201
            +SHE+I+LLKEKL EEK RR+RAE            VK+LEDELSTWKS+V EIP VS A
Sbjct: 299  SSHENIELLKEKLLEEKGRRDRAEAELLKLADIQLTVKKLEDELSTWKSMVLEIPGVSCA 358

Query: 1202 DDIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYK 1381
            DDI  KFA+LQKEV+DSM+K  EAQ  +K+++ +LD+A LDK N E E ++AK+KAE  K
Sbjct: 359  DDIPRKFASLQKEVLDSMIKMGEAQASLKQMEVALDNAKLDKSNTEAEVNLAKEKAEILK 418

Query: 1382 AEIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVX 1561
            +E+KR ELML  ++EERDRLK +V+E R   + ++G E+  G +VQELE SLAKKEN++ 
Sbjct: 419  SELKRTELMLASVSEERDRLKYIVEEPRTQKSMDTGIEVSGGKVVQELELSLAKKENFIK 478

Query: 1562 XXXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                       +N RQ NE+ LLN+RL++EARR+K LERE
Sbjct: 479  ELESSLSEQKEVNGRQLNELKLLNDRLSSEARRIKSLERE 518



 Score =  102 bits (254), Expect = 7e-19
 Identities = 55/67 (82%), Positives = 57/67 (85%), Gaps = 3/67 (4%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQS--- 1847
            E KLGHGD+SSA TKVLRMVNTL VDNE KQTI+ALQNELQK KEKL AVEELKKQS   
Sbjct: 530  ESKLGHGDYSSATTKVLRMVNTLGVDNETKQTIEALQNELQKTKEKLHAVEELKKQSDYP 589

Query: 1848 ADAGTHV 1868
            ADAGT V
Sbjct: 590  ADAGTLV 596


>ref|XP_016500353.1| PREDICTED: mitotic spindle checkpoint protein MAD1-like isoform X3
            [Nicotiana tabacum]
          Length = 602

 Score =  537 bits (1383), Expect = 0.0
 Identities = 289/517 (55%), Positives = 374/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN+D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNDDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF+++M+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHDMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQEELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNVLVKHLQEELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RRERAE           ++K+LEDEL TWKS++K+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRERAESELLKFADLQGDMKKLEDELITWKSMIKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM KA E Q  +K+++ +LD+A L+K+ AE+EA++AK+ AES K+EI
Sbjct: 357  PPKFAALQREVLDSMTKAGEVQARLKQMEVALDTAELEKRKAESEAALAKESAESSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR++L L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIKLKLAAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score = 95.5 bits (236), Expect = 8e-17
 Identities = 47/59 (79%), Positives = 54/59 (91%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSAD 1853
            E KLGHGD+SSANTKVLRMVNTL V++EAKQTI+ALQNELQK KEKL AV+ELK+QS +
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVESEAKQTIEALQNELQKTKEKLLAVQELKEQSVE 583


>ref|XP_016500351.1| PREDICTED: mitotic spindle checkpoint protein MAD1-like isoform X2
            [Nicotiana tabacum]
 ref|XP_016500352.1| PREDICTED: mitotic spindle checkpoint protein MAD1-like isoform X2
            [Nicotiana tabacum]
          Length = 605

 Score =  537 bits (1383), Expect = 0.0
 Identities = 289/517 (55%), Positives = 374/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN+D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNDDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF+++M+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHDMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQEELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNVLVKHLQEELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RRERAE           ++K+LEDEL TWKS++K+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRERAESELLKFADLQGDMKKLEDELITWKSMIKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM KA E Q  +K+++ +LD+A L+K+ AE+EA++AK+ AES K+EI
Sbjct: 357  PPKFAALQREVLDSMTKAGEVQARLKQMEVALDTAELEKRKAESEAALAKESAESSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR++L L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIKLKLAAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score = 94.7 bits (234), Expect = 1e-16
 Identities = 47/57 (82%), Positives = 53/57 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQS 1847
            E KLGHGD+SSANTKVLRMVNTL V++EAKQTI+ALQNELQK KEKL AV+ELK+QS
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVESEAKQTIEALQNELQKTKEKLLAVQELKEQS 581


>ref|XP_009791242.1| PREDICTED: early endosome antigen 1 isoform X2 [Nicotiana sylvestris]
          Length = 605

 Score =  533 bits (1373), Expect = e-180
 Identities = 287/517 (55%), Positives = 372/517 (71%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PDTSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQAHD QSK + LN D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQAHDSQSKLQTLNNDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF++EM+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHEMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQ+ELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNILVKHLQDELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RR+RAE           ++K+LEDEL+TWKS+VK+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRDRAESELLKLADLQGDMKKLEDELTTWKSMVKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE+EA++AK+ A   K+EI
Sbjct: 357  PPKFAALQREVLDSMTKVGEVQAQLKQMEVALDTAELEKRKAESEAAVAKESANFSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR+EL L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIELKLVAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score = 96.7 bits (239), Expect = 4e-17
 Identities = 48/57 (84%), Positives = 53/57 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQS 1847
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KEKL AV+ELK+QS
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEKLLAVQELKEQS 581


>ref|XP_016500350.1| PREDICTED: mitotic spindle checkpoint protein MAD1-like isoform X1
            [Nicotiana tabacum]
          Length = 720

 Score =  537 bits (1383), Expect = e-180
 Identities = 289/517 (55%), Positives = 374/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN+D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNDDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF+++M+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHDMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQEELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNVLVKHLQEELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RRERAE           ++K+LEDEL TWKS++K+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRERAESELLKFADLQGDMKKLEDELITWKSMIKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM KA E Q  +K+++ +LD+A L+K+ AE+EA++AK+ AES K+EI
Sbjct: 357  PPKFAALQREVLDSMTKAGEVQARLKQMEVALDTAELEKRKAESEAALAKESAESSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR++L L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIKLKLAAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score =  103 bits (256), Expect = 4e-19
 Identities = 52/64 (81%), Positives = 58/64 (90%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V++EAKQTI+ALQNELQK KEKL AV+ELK+QSAD 
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVESEAKQTIEALQNELQKTKEKLLAVQELKEQSADV 584

Query: 1857 GTHV 1868
            GT V
Sbjct: 585  GTLV 588


>ref|XP_009627035.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Nicotiana
            tomentosiformis]
          Length = 720

 Score =  537 bits (1383), Expect = e-180
 Identities = 289/517 (55%), Positives = 374/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN+D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNDDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF++EM+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHEMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQEELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNVLVKHLQEELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RR+RAE           ++K+LEDEL TWKS++K+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRDRAESELLKFADLQGDMKKLEDELITWKSMIKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM KA E Q  +K+++ +LD+A L+K+ AE+EA++AK+ AES K+EI
Sbjct: 357  PPKFAALQREVLDSMTKAGEVQARLKQMEVALDTAELEKRKAESEAALAKESAESSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR++L L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIKLKLAAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score =  104 bits (260), Expect = 1e-19
 Identities = 53/64 (82%), Positives = 59/64 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V++EAKQTI+ALQNELQK KEKL AV+ELK+QSADA
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVESEAKQTIEALQNELQKTKEKLLAVQELKEQSADA 584

Query: 1857 GTHV 1868
            GT V
Sbjct: 585  GTLV 588


>ref|XP_006349279.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Solanum
            tuberosum]
          Length = 721

 Score =  536 bits (1381), Expect = e-180
 Identities = 294/519 (56%), Positives = 373/519 (71%), Gaps = 2/519 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSS-SKQLLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPP  KR+A+S  PEDG     SPSS ++QL+++ED P PE SH H  P +SDQMLCTY
Sbjct: 4    RTPPF-KRKAQSNQPEDG-----SPSSGNRQLVIFEDTPLPESSHHH--PESSDQMLCTY 55

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EFFDALSSAEKQA D QSK EALN D+ KA+AERK FRD+F NAEQELAAAK
Sbjct: 56   QCRQMVKSEFFDALSSAEKQARDNQSKLEALNNDYLKADAERKKFRDQFLNAEQELAAAK 115

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GRE+ LQ+QL KE++ SQE+LRKQ+Q Y+ELEVKF+NEM+LR                  
Sbjct: 116  GREEALQDQLMKEVNVSQERLRKQLQLYNELEVKFQNEMNLRKKVETSAAAAEEKASLLE 175

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L ++SESI+REK+RLQNDL Q++SESK S+++I+ +LE ME RA NA           
Sbjct: 176  RKLSSVSESIEREKSRLQNDLEQLKSESKFSITKISNNLEIMEFRATNAEEESVLLKEQL 235

Query: 848  XXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CMQ+K E EKKLS+ +  E  S D+++LVKHLQEELR+YE+EVREARK+K+
Sbjct: 236  EELRKRLDECMQQKTEAEKKLSSFTFQEGCSSDSNILVKHLQEELRSYEAEVREARKLKS 295

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE+I+LLK K+ EEK RRERAE           ++++LEDEL+TWKS+VK+IP  S A 
Sbjct: 296  SHENIELLKVKILEEKGRRERAESELLKLAMLQGDMEKLEDELNTWKSMVKDIPGASCAA 355

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            D+ PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE EA +AK+KAES K+
Sbjct: 356  DVPPKFAALQREVLDSMTKVGEIQARLKQMEVALDAADLEKREAENEAVLAKEKAESSKS 415

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKR+EL L  + EE DRLKNV+++ R+  + ESG E+ SG I+QELE SLAKKEN +  
Sbjct: 416  EIKRIELKLASVMEETDRLKNVIEDFRKQKSVESGQEVVSGAILQELETSLAKKENCIKE 475

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                      +N RQ NEI  LNE+L NEARR+K LERE
Sbjct: 476  LESYLSEQKEVNIRQLNEIKFLNEKLNNEARRIKSLERE 514



 Score =  103 bits (258), Expect = 2e-19
 Identities = 53/64 (82%), Positives = 58/64 (90%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KE+L AV+ELK QSADA
Sbjct: 526  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEQLLAVQELKGQSADA 585

Query: 1857 GTHV 1868
            GT V
Sbjct: 586  GTLV 589


>ref|XP_015060942.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Solanum
            pennellii]
          Length = 721

 Score =  534 bits (1376), Expect = e-179
 Identities = 293/522 (56%), Positives = 376/522 (72%), Gaps = 2/522 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSS-SKQLLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPP  KR+A+S  PEDG     SPSS ++QL+++ED P PE SH H  P +S+QMLCTY
Sbjct: 4    RTPPF-KRKAQSNQPEDG-----SPSSGNQQLVIFEDTPLPESSHHH--PESSEQMLCTY 55

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EFFDALSSAEKQA D QSK EALN D+ KA+AERK FRD+F NAEQELAAAK
Sbjct: 56   QCRQMVKSEFFDALSSAEKQARDNQSKLEALNNDYLKADAERKKFRDQFLNAEQELAAAK 115

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GRE+ LQ+QL KE++ SQE+LRKQ+Q Y+ELEVKF+NEM+LR                  
Sbjct: 116  GREEALQDQLMKEVNVSQERLRKQLQLYNELEVKFQNEMNLRKKVETSAAAAEEKASLLE 175

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L ++SESI+REK+RLQNDL Q++SESK S+++I+ +LE+ME RA NA           
Sbjct: 176  RKLSSVSESIEREKSRLQNDLEQLKSESKFSITKISNNLERMEFRATNAEEESVLLKEQL 235

Query: 848  XXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CMQ+K E EKKLS+ +  E  S D+++LVKHLQEELR+YE+EVREARK+K+
Sbjct: 236  EELRKRLDECMQQKTEAEKKLSSFTFQEGCSSDSNILVKHLQEELRSYEAEVREARKLKS 295

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE+I+LLK  + EEK+RRERAE           ++++LEDEL+ WKS+VK+IP  SSA 
Sbjct: 296  SHENIELLKVTILEEKSRRERAESELLKLAVLQGDMEKLEDELNAWKSIVKDIPGASSAA 355

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            D+ PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE EA +AK+KAES K+
Sbjct: 356  DVPPKFAALQREVLDSMTKVGEIQARLKQMEVALDAADLEKKEAENEAVLAKEKAESSKS 415

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKR+EL L  + EE DRLKNV+++LR+  + ESG E+ SG I+QELEA LAKKEN +  
Sbjct: 416  EIKRIELKLASVMEETDRLKNVIEDLRKQKSVESGHEVVSGAILQELEAFLAKKENCIKE 475

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLEREEAG 1690
                      +N RQ NEI  LNE+L +EARR+K LERE  G
Sbjct: 476  LESYLSEQKEVNIRQLNEIKFLNEKLNSEARRIKSLEREGDG 517



 Score =  103 bits (258), Expect = 2e-19
 Identities = 53/64 (82%), Positives = 58/64 (90%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KE+L AV+ELK QSADA
Sbjct: 526  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEQLLAVQELKGQSADA 585

Query: 1857 GTHV 1868
            GT V
Sbjct: 586  GTLV 589


>ref|XP_019230208.1| PREDICTED: mitotic spindle checkpoint protein MAD1 isoform X2
            [Nicotiana attenuata]
          Length = 606

 Score =  530 bits (1365), Expect = e-179
 Identities = 286/517 (55%), Positives = 373/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN D+ KA+AERK FRD+F N+EQELAAA+G
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNNDYLKADAERKKFRDQFLNSEQELAAARG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF++E++LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHEVNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIKREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQ+ELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNILVKHLQDELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RR+RAE           ++K+LEDEL+TWKS+VK+IP VS ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRDRAESELLKLADLQGDMKKLEDELTTWKSMVKDIPGVSCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE+EA++AK+ A+S K+EI
Sbjct: 357  PPKFAALQREVLDSMTKVGEVQARLKQMEVALDTAELEKRKAESEAAVAKESADSSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR+EL L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKE+ +    
Sbjct: 417  KRIELKLVAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKEDAIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score = 96.3 bits (238), Expect = 5e-17
 Identities = 47/57 (82%), Positives = 53/57 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQS 1847
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KEKL A++ELK+QS
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEKLLAIQELKEQS 581


>ref|XP_009791238.1| PREDICTED: spindle assembly checkpoint component mad1 isoform X1
            [Nicotiana sylvestris]
 ref|XP_016506347.1| PREDICTED: mitotic spindle checkpoint protein MAD1-like [Nicotiana
            tabacum]
          Length = 720

 Score =  533 bits (1373), Expect = e-179
 Identities = 287/517 (55%), Positives = 372/517 (71%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PDTSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQAHD QSK + LN D+ KA+AERK FRD+F N+EQELAAAKG
Sbjct: 57   CRQMVKSEFFDALSSAEKQAHDSQSKLQTLNNDYLKADAERKKFRDQFLNSEQELAAAKG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF++EM+LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHEMNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI+REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIEREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQ+ELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNILVKHLQDELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RR+RAE           ++K+LEDEL+TWKS+VK+IP  S ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRDRAESELLKLADLQGDMKKLEDELTTWKSMVKDIPGASCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE+EA++AK+ A   K+EI
Sbjct: 357  PPKFAALQREVLDSMTKVGEVQAQLKQMEVALDTAELEKRKAESEAAVAKESANFSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR+EL L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKEN +    
Sbjct: 417  KRIELKLVAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKENSIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score =  106 bits (265), Expect = 3e-20
 Identities = 54/64 (84%), Positives = 59/64 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KEKL AV+ELK+QSADA
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEKLLAVQELKEQSADA 584

Query: 1857 GTHV 1868
            GT V
Sbjct: 585  GTLV 588


>ref|XP_019230207.1| PREDICTED: mitotic spindle checkpoint protein MAD1 isoform X1
            [Nicotiana attenuata]
          Length = 720

 Score =  530 bits (1365), Expect = e-177
 Identities = 286/517 (55%), Positives = 373/517 (72%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSSSKQLLVYEDLPAPEYSHDHSTPITSDQMLCTYQ 310
            RTPP  KRRA+S+ PEDG+ N    S ++QL++YED P PE SHDH  P TSDQMLCTYQ
Sbjct: 4    RTPPL-KRRAQSKQPEDGSPN----SGNRQLIIYEDTPLPESSHDH--PETSDQMLCTYQ 56

Query: 311  CRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAKG 490
            CRQMVK+EFFDALSSAEKQA D QSK E LN D+ KA+AERK FRD+F N+EQELAAA+G
Sbjct: 57   CRQMVKSEFFDALSSAEKQARDSQSKLETLNNDYLKADAERKKFRDQFLNSEQELAAARG 116

Query: 491  REQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXXX 670
            RE+ LQ+QL KE++ SQE+LRKQ+Q YSELE KF++E++LR                   
Sbjct: 117  REEALQDQLLKEVNASQERLRKQLQLYSELEGKFQHEVNLRKKAETSAAASEEKASLLER 176

Query: 671  NLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXXX 850
             L ++SESI REK+RLQNDL Q++SESK SV++I+ +LE+ME RA NA            
Sbjct: 177  KLSSVSESIKREKSRLQNDLEQLKSESKFSVTKISKNLERMEFRAANAEKESVLLKEQLE 236

Query: 851  XXXXXXXXCMQEKFEVEKKLSAKSPETPSKDTSLLVKHLQEELRNYESEVREARKIKASH 1030
                    C+Q++ E EKKLS  + +      ++LVKHLQ+ELRN E+EVREARK+++SH
Sbjct: 237  ELRKRLDECVQQRIEAEKKLSNFTFQEGCSSDNILVKHLQDELRNCEAEVREARKLRSSH 296

Query: 1031 EDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSADDI 1210
            E+I+LLK KL EEK RR+RAE           ++K+LEDEL+TWKS+VK+IP VS ADD+
Sbjct: 297  ENIELLKVKLLEEKGRRDRAESELLKLADLQGDMKKLEDELTTWKSMVKDIPGVSCADDV 356

Query: 1211 IPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKAEI 1390
             PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE+EA++AK+ A+S K+EI
Sbjct: 357  PPKFAALQREVLDSMTKVGEVQARLKQMEVALDTAELEKRKAESEAAVAKESADSSKSEI 416

Query: 1391 KRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXXXX 1570
            KR+EL L  + EE+DRLKNVV++LR+  + ESG E+ SG I+Q LEASLAKKE+ +    
Sbjct: 417  KRIELKLVAVIEEKDRLKNVVEDLRKQKSVESGHEVVSGAILQGLEASLAKKEDAIKELE 476

Query: 1571 XXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLERE 1681
                    +N RQ NEI LL+E+L NEARR++ LERE
Sbjct: 477  SSLSEQKEVNIRQLNEINLLSEKLNNEARRIRSLERE 513



 Score =  106 bits (264), Expect = 4e-20
 Identities = 53/64 (82%), Positives = 59/64 (92%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KEKL A++ELK+QSADA
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEKLLAIQELKEQSADA 584

Query: 1857 GTHV 1868
            GT V
Sbjct: 585  GTLV 588


>ref|XP_016538948.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Capsicum annuum]
          Length = 720

 Score =  530 bits (1365), Expect = e-177
 Identities = 289/522 (55%), Positives = 370/522 (70%), Gaps = 2/522 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSS-SKQLLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTP   KR+A+S+ PEDG     SPSS +++L++YED P PE SH H  P T DQMLCTY
Sbjct: 4    RTPV--KRKAQSKQPEDG-----SPSSGNRKLVIYEDTPLPESSHHH--PQTDDQMLCTY 54

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EFFDALSSAEKQA D QSK E LN D+ KA+AERK FRD+F NAEQELAAAK
Sbjct: 55   QCRQMVKSEFFDALSSAEKQARDNQSKLETLNNDYLKADAERKKFRDQFLNAEQELAAAK 114

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GRE+ LQ+QL KE++ SQE+L+KQ+Q Y+ELE KF+NEM+LR                  
Sbjct: 115  GREEALQDQLMKEVNVSQERLKKQLQLYNELEAKFQNEMNLRKKAETSAAAAEEKASLLE 174

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L ++SESI+RE++RL N+L Q++SESK SV++I+  LE+ME RA N+           
Sbjct: 175  RKLSSVSESIERERSRLHNELEQLKSESKFSVTKISNSLERMEFRATNSEKESVLLKEQL 234

Query: 848  XXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CMQ+K E EKKLS  +  E  S D ++LVKHLQEELR+YE+EVREARK+K+
Sbjct: 235  EELRKRLDECMQQKIEAEKKLSNFTFQEGFSSDNNILVKHLQEELRSYEAEVREARKLKS 294

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE+++LLKEK+ EEK RRERAE           ++++LEDEL+ WKSVVK+IP VS A 
Sbjct: 295  SHENVELLKEKILEEKGRRERAESELLKLAVLQGDIEKLEDELNAWKSVVKDIPGVSCAA 354

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            D+ PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ A+ EA +AK+KAES K+
Sbjct: 355  DVPPKFAALQREVLDSMTKVGEIQARLKQMEVALDAAELEKREADNEAVLAKEKAESSKS 414

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKR+EL L  + EERDRLKNV++  R+  + ESG E+ SG ++QELEASLAKKEN +  
Sbjct: 415  EIKRIELKLASVMEERDRLKNVIEAFRKQKSVESGHEVVSGAVLQELEASLAKKENCIKE 474

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLEREEAG 1690
                      +N RQ NEI  LNE+L NEARR+K LERE  G
Sbjct: 475  LESYLSEQKEVNIRQLNEIKFLNEKLNNEARRIKSLEREGDG 516



 Score =  101 bits (251), Expect = 2e-18
 Identities = 51/64 (79%), Positives = 57/64 (89%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQ ELQK KE+L AV+ELK+QSADA
Sbjct: 525  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQKELQKTKEQLLAVQELKEQSADA 584

Query: 1857 GTHV 1868
            G  V
Sbjct: 585  GAMV 588


>ref|XP_004230415.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Solanum
            lycopersicum]
          Length = 721

 Score =  529 bits (1363), Expect = e-177
 Identities = 291/522 (55%), Positives = 373/522 (71%), Gaps = 2/522 (0%)
 Frame = +2

Query: 131  RTPPARKRRAESQPPEDGNNNTHSPSS-SKQLLVYEDLPAPEYSHDHSTPITSDQMLCTY 307
            RTPP  KR+A+S  PEDG     SPSS ++QL+++ED P PE SH H  P +S+QMLCTY
Sbjct: 4    RTPPF-KRKAQSNQPEDG-----SPSSGNQQLVIFEDTPLPESSHHH--PESSEQMLCTY 55

Query: 308  QCRQMVKAEFFDALSSAEKQAHDYQSKFEALNEDFSKAEAERKNFRDKFFNAEQELAAAK 487
            QCRQMVK+EFFDALSSAEKQA D QSK EALN D+ KA+AERK FRD+F NAEQELAAAK
Sbjct: 56   QCRQMVKSEFFDALSSAEKQARDNQSKLEALNNDYLKADAERKKFRDQFLNAEQELAAAK 115

Query: 488  GREQMLQEQLRKEIDFSQEQLRKQIQSYSELEVKFRNEMDLRXXXXXXXXXXXXXXXXXX 667
            GRE+ LQ+QL KE++ S E+LRKQ+Q Y+ELEVKF+NEM+LR                  
Sbjct: 116  GREEALQDQLMKEVNVSHERLRKQLQLYNELEVKFQNEMNLRKKVETSAAAAEEKASLLE 175

Query: 668  XNLRTLSESIDREKNRLQNDLVQMRSESKLSVSRITADLEKMECRAKNAXXXXXXXXXXX 847
              L ++SESI+REK+RLQNDL  ++SESK S+++I+ +LE+ME RA NA           
Sbjct: 176  RKLSSVSESIEREKSRLQNDLELLKSESKFSITKISNNLERMEFRATNAEEESVLLKEQL 235

Query: 848  XXXXXXXXXCMQEKFEVEKKLSAKS-PETPSKDTSLLVKHLQEELRNYESEVREARKIKA 1024
                     CMQ+K E EKKLS+ +  E  S D+++LVKHLQEELR+YE+EVREARK+K+
Sbjct: 236  EELRKRLDECMQQKTEAEKKLSSFTFQEGCSSDSNILVKHLQEELRSYEAEVREARKLKS 295

Query: 1025 SHEDIQLLKEKLYEEKNRRERAEXXXXXXXXXXXNVKRLEDELSTWKSVVKEIPAVSSAD 1204
            SHE+I+LLK  + EEK RRERAE           ++++LEDEL+ WKS+VK+IP  S A 
Sbjct: 296  SHENIELLKITILEEKGRRERAESELLKLAVLQGDMEKLEDELNAWKSIVKDIPGASCAA 355

Query: 1205 DIIPKFAALQKEVVDSMMKASEAQVCMKEIQASLDSAVLDKQNAETEASMAKDKAESYKA 1384
            D+ PKFAALQ+EV+DSM K  E Q  +K+++ +LD+A L+K+ AE EA +AK+KAES K+
Sbjct: 356  DVPPKFAALQREVLDSMTKVGEIQARLKQMEVALDAADLEKKEAENEAVLAKEKAESSKS 415

Query: 1385 EIKRLELMLGLITEERDRLKNVVQELREPTNRESGTELFSGTIVQELEASLAKKENYVXX 1564
            EIKR+EL L  + EE DRLKNV+++LR+  + ESG E+ SG I+QELEASLAKKEN +  
Sbjct: 416  EIKRIELKLASVMEETDRLKNVIEDLRKQKSVESGHEVVSGAILQELEASLAKKENCIKE 475

Query: 1565 XXXXXXXXXXINSRQHNEIMLLNERLTNEARRVKMLEREEAG 1690
                      +N RQ NEI  LNE+L +EARR+K LERE  G
Sbjct: 476  LESYLSEQKEVNIRQLNEIKFLNEKLNSEARRIKSLEREGDG 517



 Score =  103 bits (258), Expect = 2e-19
 Identities = 53/64 (82%), Positives = 58/64 (90%)
 Frame = +3

Query: 1677 EKKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIDALQNELQKAKEKLQAVEELKKQSADA 1856
            E KLGHGD+SSANTKVLRMVNTL V+NEAKQTI+ALQNELQK KE+L AV+ELK QSADA
Sbjct: 526  ESKLGHGDYSSANTKVLRMVNTLGVENEAKQTIEALQNELQKTKEQLLAVQELKGQSADA 585

Query: 1857 GTHV 1868
            GT V
Sbjct: 586  GTLV 589