BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia29_contig00000593
         (1841 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011083688.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   962   0.0  
gb|PIN03748.1| Fe-S oxidoreductase [Handroanthus impetiginosus]       951   0.0  
ref|XP_012845981.1| PREDICTED: S-adenosyl-L-methionine-dependent...   935   0.0  
ref|XP_022869688.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   914   0.0  
gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   909   0.0  
ref|XP_019156453.1| PREDICTED: S-adenosyl-L-methionine-dependent...   878   0.0  
ref|NP_001347563.1| uncharacterized LOC111946218 [Nicotiana tome...   870   0.0  
ref|XP_016449047.1| PREDICTED: S-adenosyl-L-methionine-dependent...   868   0.0  
ref|XP_021601254.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   847   0.0  
ref|XP_021667680.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   844   0.0  
ref|XP_012071843.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   842   0.0  
ref|XP_018624471.1| PREDICTED: uncharacterized protein LOC104090...   870   0.0  
ref|NP_001347568.1| uncharacterized protein LOC111946220 [Ricinu...   835   0.0  
ref|XP_022772746.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   834   0.0  
ref|XP_015884714.1| PREDICTED: S-adenosyl-L-methionine-dependent...   834   0.0  
ref|XP_020410607.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   832   0.0  
ref|XP_021900738.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   832   0.0  
ref|XP_008220799.1| PREDICTED: S-adenosyl-L-methionine-dependent...   831   0.0  
ref|XP_021826483.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   828   0.0  
ref|XP_018821629.1| PREDICTED: S-adenosyl-L-methionine-dependent...   827   0.0  

>ref|XP_011083688.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Sesamum indicum]
          Length = 660

 Score =  962 bits (2487), Expect = 0.0
 Identities = 463/536 (86%), Positives = 487/536 (90%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            FLVNWL ESADDFRVGAL++KECKYAVFGVGS+SYGETYNAVARG+SVKLRKLGASELVE
Sbjct: 125  FLVNWLTESADDFRVGALVLKECKYAVFGVGSRSYGETYNAVARGVSVKLRKLGASELVE 184

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            LGEGDVDE ++++ FD W + +VGVLK   GENG HFENYGV                  
Sbjct: 185  LGEGDVDEADLDEVFDLWSKKVVGVLKDKLGENGQHFENYGVECESEGEGEFSEEDDDED 244

Query: 1477 XXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGYK 1298
                    IVDLEDIAGKGPSRRS++  KANGK+NG VLNGEKEMVTPV+RANLEKQGYK
Sbjct: 245  AENGGDSAIVDLEDIAGKGPSRRSVVATKANGKVNGEVLNGEKEMVTPVLRANLEKQGYK 304

Query: 1297 VIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 1118
            +IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNP
Sbjct: 305  IIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNP 364

Query: 1117 VGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPIM 938
            VGKSW+WKMDDPLVIVDTAI+LHTKMI+QMKGVPGVKAE LSEGLSPRHCALSLVGEPIM
Sbjct: 365  VGKSWKWKMDDPLVIVDTAIELHTKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIM 424

Query: 937  YPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFS 758
            YPEIN+LVDELHRRRISTFLVTNAQFPE+IKMLKPITQLYVSVDAATKDSLKAIDRPLF 
Sbjct: 425  YPEINALVDELHRRRISTFLVTNAQFPERIKMLKPITQLYVSVDAATKDSLKAIDRPLFG 484

Query: 757  DFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCGS 578
            DFWERF+DSLK+LREKQQRTVYRLTLVKGWNTEDVDAYS LFDVG PDFIEIKGVTYCGS
Sbjct: 485  DFWERFIDSLKSLREKQQRTVYRLTLVKGWNTEDVDAYSSLFDVGSPDFIEIKGVTYCGS 544

Query: 577  SATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWYT 398
            SATSKLTMENVPWHSDVKEFSEALAQKSNG YEVACEHVHSCCVLLAKVDKFKV+GQWYT
Sbjct: 545  SATSKLTMENVPWHSDVKEFSEALAQKSNGMYEVACEHVHSCCVLLAKVDKFKVNGQWYT 604

Query: 397  WIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            WIDYDKFHDLVSAGEPFTSKDYMA TP WAVYGAEEGGFDP QSRFRKER HKS+R
Sbjct: 605  WIDYDKFHDLVSAGEPFTSKDYMAPTPSWAVYGAEEGGFDPLQSRFRKERHHKSSR 660


>gb|PIN03748.1| Fe-S oxidoreductase [Handroanthus impetiginosus]
          Length = 664

 Score =  951 bits (2459), Expect = 0.0
 Identities = 459/540 (85%), Positives = 487/540 (90%)
 Frame = -2

Query: 1840 AFLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELV 1661
            AFLVNWLA+SADDFRVGAL +KECKYAVFGVGS+SYGETYNAVAR +S+KLRKLGA ELV
Sbjct: 124  AFLVNWLADSADDFRVGALALKECKYAVFGVGSRSYGETYNAVARSVSLKLRKLGAGELV 183

Query: 1660 ELGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            +LGEGDVDEGN+++ FDRW   IVGVLKGN GENGWH +NYGV                 
Sbjct: 184  DLGEGDVDEGNLDEVFDRWSTKIVGVLKGNLGENGWHSKNYGVVSESEGDGEFSEEDDAE 243

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                     IVDLEDIAGK PSRRS + AKANGK+NG VLNGEKEMVTPVIRANLEKQGY
Sbjct: 244  AGDGNESG-IVDLEDIAGKAPSRRSAVAAKANGKVNGQVLNGEKEMVTPVIRANLEKQGY 302

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 303  KIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 362

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSWQWKMDDPLVIVDTAIDLHTKMI+QMKGVPGVKAE LSEGLSP+HCALSLVGEPI
Sbjct: 363  PVGKSWQWKMDDPLVIVDTAIDLHTKMIRQMKGVPGVKAERLSEGLSPKHCALSLVGEPI 422

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEINSL+ ELHRRRISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATKDSLKAIDRPLF
Sbjct: 423  MYPEINSLIAELHRRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPLF 482

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERFVDSLKAL +KQQRTVYRLTLVKGWN EDVDAYS LFDVG+PDFIEIKGVTYCG
Sbjct: 483  GDFWERFVDSLKALNKKQQRTVYRLTLVKGWNAEDVDAYSSLFDVGNPDFIEIKGVTYCG 542

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            +SATSK+TMENVPWHSDVKEFSEALA+KS GAYEVACEH HSCCVLLAKV+KFKV+GQW+
Sbjct: 543  TSATSKITMENVPWHSDVKEFSEALARKSKGAYEVACEHAHSCCVLLAKVEKFKVNGQWH 602

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR*AG 221
            TWIDYDKFHDLV++GEPF+SKDY   TP WAVYGAEEGGFDPQQSRFRKER HKSAR AG
Sbjct: 603  TWIDYDKFHDLVASGEPFSSKDYTLPTPSWAVYGAEEGGFDPQQSRFRKERHHKSARPAG 662


>ref|XP_012845981.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Erythranthe guttata]
 gb|EYU45151.1| hypothetical protein MIMGU_mgv1a002531mg [Erythranthe guttata]
          Length = 662

 Score =  935 bits (2417), Expect = 0.0
 Identities = 451/533 (84%), Positives = 483/533 (90%)
 Frame = -2

Query: 1840 AFLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELV 1661
            AFLVNWLAESA+DFRVGAL++KEC+YAVFGVGS+SYGETYNAVARG+SVKLRKLGA ELV
Sbjct: 125  AFLVNWLAESAEDFRVGALVLKECRYAVFGVGSRSYGETYNAVARGVSVKLRKLGAGELV 184

Query: 1660 ELGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            ELGEGDVDEG++++ FD+W + +VGVL GN GEN    +N  V                 
Sbjct: 185  ELGEGDVDEGDLDEIFDQWSKKVVGVLNGNLGENEGPSDNCEVASESEGEEGEYSEEDDE 244

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                     +VDLEDIAGKGPSR+S  + KANGK+NG V NG KEMVTPVIRANLEKQGY
Sbjct: 245  DEEVEENG-VVDLEDIAGKGPSRKSGAVVKANGKVNGQVSNGVKEMVTPVIRANLEKQGY 303

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 304  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 363

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGK+WQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELL+EGLSPRHCALSLVGEPI
Sbjct: 364  PVGKNWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLAEGLSPRHCALSLVGEPI 423

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEINSLVDELH+RRISTFLVTNAQFP+KIK+L+PITQLYVSVDAATKDSLKAIDRPLF
Sbjct: 424  MYPEINSLVDELHKRRISTFLVTNAQFPDKIKLLRPITQLYVSVDAATKDSLKAIDRPLF 483

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERFVDSLKAL++KQQRTVYRLTLVKGWNTED+DAYS LFDVG PDFIEIKGVTYCG
Sbjct: 484  GDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEDLDAYSSLFDVGVPDFIEIKGVTYCG 543

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWH+DVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKV+KFKVDG+WY
Sbjct: 544  SSATSKLTMENVPWHADVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVEKFKVDGEWY 603

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRH 242
            TWIDYDKFHDLV AGEPFTSKDYMA TP WAVYGAEEGGFDPQQSRFRKERRH
Sbjct: 604  TWIDYDKFHDLVEAGEPFTSKDYMARTPSWAVYGAEEGGFDPQQSRFRKERRH 656


>ref|XP_022869688.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Olea europaea var. sylvestris]
          Length = 659

 Score =  914 bits (2361), Expect = 0.0
 Identities = 444/537 (82%), Positives = 477/537 (88%), Gaps = 1/537 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            FLVNWLAESADDFRVGALL++ CK+AVFG+GS SYGET+NAVAR ISVKLRKLGA++LV+
Sbjct: 124  FLVNWLAESADDFRVGALLLRNCKFAVFGIGSSSYGETFNAVARDISVKLRKLGAADLVD 183

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            LGEGDVDEGN+++ F+RW   IVG+LKGN GEN     NYGV                  
Sbjct: 184  LGEGDVDEGNLDEVFERWSERIVGILKGNLGENKALNGNYGVGNESEGEVEFSEEEDEEE 243

Query: 1477 XXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKL-NGHVLNGEKEMVTPVIRANLEKQGY 1301
                    IVDLEDI GK PSR+S++  K NGK+ NG VLNG KEMVTPVIRA+L KQGY
Sbjct: 244  DGEGDNG-IVDLEDIVGKAPSRKSIVGPKTNGKVENGEVLNGGKEMVTPVIRASLVKQGY 302

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 303  KIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 362

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSW+WKMDDP+VIVDTAIDLHTKMIKQMKGVPGVK E LSEGLSPRHCALSLVGEPI
Sbjct: 363  PVGKSWRWKMDDPIVIVDTAIDLHTKMIKQMKGVPGVKVERLSEGLSPRHCALSLVGEPI 422

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEINSLVDELHRRRISTFLVTNAQFP++IKMLKPITQLYVSVDAATKDSLKAIDRPLF
Sbjct: 423  MYPEINSLVDELHRRRISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKDSLKAIDRPLF 482

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERFVDSL+AL+EKQQRTVYRLTLVKGWNTEDVDAYS LF +GDPDFIEIKGVTYCG
Sbjct: 483  GDFWERFVDSLQALKEKQQRTVYRLTLVKGWNTEDVDAYSNLFVIGDPDFIEIKGVTYCG 542

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWHSDVKEFSEALAQKSNG YEVACEHVHSCCVLLA V+KFKV+ QW+
Sbjct: 543  SSATSKLTMENVPWHSDVKEFSEALAQKSNGVYEVACEHVHSCCVLLANVNKFKVNDQWF 602

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            TWIDYDKFHDLV+AGEPF+SKDYMA TP WAVYGAEEGGFDP+QSRF+KER HKS R
Sbjct: 603  TWIDYDKFHDLVAAGEPFSSKDYMASTPSWAVYGAEEGGFDPEQSRFKKERHHKSTR 659


>gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dorcoceras hygrometricum]
          Length = 658

 Score =  909 bits (2350), Expect = 0.0
 Identities = 439/537 (81%), Positives = 477/537 (88%), Gaps = 1/537 (0%)
 Frame = -2

Query: 1840 AFLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELV 1661
            +FLVNWL ESADDFRVGAL++K+CKYAVFGVGS SY ETYNAVA+G+S  LRKLGA ELV
Sbjct: 117  SFLVNWLTESADDFRVGALVLKDCKYAVFGVGSSSYVETYNAVAKGVSENLRKLGAIELV 176

Query: 1660 ELGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            ELGEGD +EGN+++ F  W   +VGVLKG+ GEN  HFEN                    
Sbjct: 177  ELGEGDREEGNLDEVFHGWSDRVVGVLKGSIGENLGHFENGEAGSKIEGEFEFSDSEEEE 236

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    G+VDLEDIAGKGPSR+S+M   ANGK+NG V+NGEKEMVTPVIRA+L+KQGY
Sbjct: 237  ETEDDPESGVVDLEDIAGKGPSRKSIMGTTANGKVNGEVVNGEKEMVTPVIRASLQKQGY 296

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 297  KIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 356

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSW+WKMDDPLVIVDTAIDLHTKMI+QMKGVPGVKAE LSEGLSPRHCALSLVGEPI
Sbjct: 357  PVGKSWRWKMDDPLVIVDTAIDLHTKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPI 416

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN+L+DELHRRRISTFLVTNAQFPE+IK LKPITQLYVSVDAATKDSLKAIDRPLF
Sbjct: 417  MYPEINALIDELHRRRISTFLVTNAQFPERIKTLKPITQLYVSVDAATKDSLKAIDRPLF 476

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERFV+SLKALREKQQRTVYRLTLVKGWNTED+DAYS LFD+G+PDFIEIKGVTYCG
Sbjct: 477  GDFWERFVESLKALREKQQRTVYRLTLVKGWNTEDIDAYSSLFDLGNPDFIEIKGVTYCG 536

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWHSDVK FSEALAQKSNGAYEVACEH HSCCVLL KV KFKV+G+W+
Sbjct: 537  SSATSKLTMENVPWHSDVKNFSEALAQKSNGAYEVACEHEHSCCVLLGKVSKFKVEGEWF 596

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERR-HKSA 233
            TWIDYDKFHDLV++G+PFTS+DYM+ TP WAVYGAEEGGFDP QSRF+KERR HKSA
Sbjct: 597  TWIDYDKFHDLVASGKPFTSEDYMSPTPRWAVYGAEEGGFDPDQSRFKKERRHHKSA 653


>ref|XP_019156453.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Ipomoea nil]
          Length = 655

 Score =  878 bits (2268), Expect = 0.0
 Identities = 421/537 (78%), Positives = 470/537 (87%), Gaps = 1/537 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            FL NWL ES+DDFRVGALL+ +CK+A+FGVGS+SYGET+NAVA+  S +LRKLG +E++ 
Sbjct: 120  FLGNWLTESSDDFRVGALLLSKCKFAIFGVGSRSYGETFNAVAKDFSKRLRKLGGAEVLG 179

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGN-FGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            + EGDVDEGNV++ FD WC+ +  +LKGN  GENG    N GV                 
Sbjct: 180  VCEGDVDEGNVDEVFDEWCKRLARILKGNNCGENGRCSSN-GVVEESDGEVSDYDDYDSE 238

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    GIVDLEDIAGK PS ++M+  KANGK NG  LNGEKEMVTPVIRA+LEKQGY
Sbjct: 239  IEENGEDNGIVDLEDIAGKAPSSKTMVKPKANGKANGETLNGEKEMVTPVIRASLEKQGY 298

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 299  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 358

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSW+WKMD PL IV+TAI+LH+KMIKQMKGVPGVKA+ L+EGLSPRHCALSLVGEPI
Sbjct: 359  PVGKSWRWKMDAPLEIVETAIELHSKMIKQMKGVPGVKADRLAEGLSPRHCALSLVGEPI 418

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEINSLVDELHR+RISTFLVTNAQFPE+I MLKPITQLYVSVDAATK+SLKAIDRPLF
Sbjct: 419  MYPEINSLVDELHRKRISTFLVTNAQFPERIMMLKPITQLYVSVDAATKESLKAIDRPLF 478

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLKAL+EKQQRTVYRLTLVKGWNTEDVDAYS LF +G+PDFIEIKGVTYCG
Sbjct: 479  GDFWERFMDSLKALKEKQQRTVYRLTLVKGWNTEDVDAYSSLFSIGNPDFIEIKGVTYCG 538

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWHSDVKEFSEALA+KSNG YEVACEHVHSCCVLLAKV+KFK+DGQW+
Sbjct: 539  SSATSKLTMENVPWHSDVKEFSEALAKKSNGEYEVACEHVHSCCVLLAKVEKFKIDGQWF 598

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            TWIDY+KFHDLV++G+PF S DYMA TP WAVYGAEEGGFDP+QSR++KER H+S R
Sbjct: 599  TWIDYEKFHDLVASGKPFRSGDYMAPTPSWAVYGAEEGGFDPEQSRYKKERHHRSTR 655


>ref|NP_001347563.1| uncharacterized LOC111946218 [Nicotiana tomentosiformis]
          Length = 661

 Score =  870 bits (2249), Expect = 0.0
 Identities = 421/544 (77%), Positives = 468/544 (86%), Gaps = 8/544 (1%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F ++WL ES DDFRVG+LL+ +CK+A+FGVGS SYGET+NAVAR  S KLR+LG  E++E
Sbjct: 115  FFIDWLNESVDDFRVGSLLLSKCKFAIFGVGSGSYGETFNAVARDFSKKLRQLGGVEVLE 174

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            +GEGDVDEGN+++ F +W + +VGVL  N G+NG +  N GV                  
Sbjct: 175  VGEGDVDEGNLDEVFGKWSKRVVGVLS-NLGQNGGYMVN-GVGDGNEDEVVSEGDDDDDD 232

Query: 1477 XXXXXXXG-------IVDLEDIAGKGPSRRSMMLAK-ANGKLNGHVLNGEKEMVTPVIRA 1322
                           I+DLEDIAGKGPSR+  +  K  NGKLNG ++NGEKEMVTPVIRA
Sbjct: 233  DDDDDEFEENDEGSEILDLEDIAGKGPSRKKGVGDKLVNGKLNGDIVNGEKEMVTPVIRA 292

Query: 1321 NLEKQGYKVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 1142
            +L KQGYK+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF
Sbjct: 293  SLVKQGYKIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 352

Query: 1141 CWRHHTNPVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCAL 962
            CWRHHTNPVGKSW+WKMDDP+ IVDTAIDLHTKMIKQMKGVPGVKAE LSEGLSPRHCAL
Sbjct: 353  CWRHHTNPVGKSWRWKMDDPIEIVDTAIDLHTKMIKQMKGVPGVKAERLSEGLSPRHCAL 412

Query: 961  SLVGEPIMYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLK 782
            SLVGEPIMYPEINSLVDELH+RRISTFLVTNAQFP+KIK+LKPITQLYVSVDA TKDSLK
Sbjct: 413  SLVGEPIMYPEINSLVDELHKRRISTFLVTNAQFPDKIKLLKPITQLYVSVDAGTKDSLK 472

Query: 781  AIDRPLFSDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEI 602
            AIDRPLF DFWERF+DSLKAL+EK+QRTVYRLTLVKGWN E+VDAY  LF +G PDFIEI
Sbjct: 473  AIDRPLFGDFWERFLDSLKALKEKEQRTVYRLTLVKGWNAEEVDAYFSLFSIGKPDFIEI 532

Query: 601  KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKF 422
            KGVTYCGSSATSKLTMENVPWHSDVKEFSEALA+KSNG YEVACEHVHSCCVLLAKVDKF
Sbjct: 533  KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAKKSNGGYEVACEHVHSCCVLLAKVDKF 592

Query: 421  KVDGQWYTWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRH 242
            K+DGQWYTWIDY+KFHDLV++G+PFTSKDYMA TP WAVYGAEEGGFDP+Q R++KER H
Sbjct: 593  KIDGQWYTWIDYEKFHDLVASGKPFTSKDYMAPTPSWAVYGAEEGGFDPEQLRYKKERHH 652

Query: 241  KSAR 230
            KS+R
Sbjct: 653  KSSR 656


>ref|XP_016449047.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Nicotiana tabacum]
          Length = 661

 Score =  868 bits (2244), Expect = 0.0
 Identities = 420/544 (77%), Positives = 467/544 (85%), Gaps = 8/544 (1%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F ++WL ES DDFRVG+LL+ +CK+A+FGVGS SYGET+NAVAR  S KLR+LG  E++E
Sbjct: 115  FFIDWLNESVDDFRVGSLLLSKCKFAIFGVGSGSYGETFNAVARDFSKKLRQLGGVEVLE 174

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            +GEGDVDEGN+++ F +W + +VGV   N G+NG +  N GV                  
Sbjct: 175  VGEGDVDEGNLDEVFGKWSKRVVGVWS-NLGQNGGYMVN-GVGDGNEDEVVSEGDDDDDD 232

Query: 1477 XXXXXXXG-------IVDLEDIAGKGPSRRSMMLAK-ANGKLNGHVLNGEKEMVTPVIRA 1322
                           I+DLEDIAGKGPSR+  +  K  NGKLNG ++NGEKEMVTPVIRA
Sbjct: 233  DDDDDEFEENDEGSEILDLEDIAGKGPSRKKGVGDKLVNGKLNGDIVNGEKEMVTPVIRA 292

Query: 1321 NLEKQGYKVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 1142
            +L KQGYK+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF
Sbjct: 293  SLVKQGYKIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 352

Query: 1141 CWRHHTNPVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCAL 962
            CWRHHTNPVGKSW+WKMDDP+ IVDTAIDLHTKMIKQMKGVPGVKAE LSEGLSPRHCAL
Sbjct: 353  CWRHHTNPVGKSWRWKMDDPIEIVDTAIDLHTKMIKQMKGVPGVKAERLSEGLSPRHCAL 412

Query: 961  SLVGEPIMYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLK 782
            SLVGEPIMYPEINSLVDELH+RRISTFLVTNAQFP+KIK+LKPITQLYVSVDA TKDSLK
Sbjct: 413  SLVGEPIMYPEINSLVDELHKRRISTFLVTNAQFPDKIKLLKPITQLYVSVDAGTKDSLK 472

Query: 781  AIDRPLFSDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEI 602
            AIDRPLF DFWERF+DSLKAL+EK+QRTVYRLTLVKGWN E+VDAY  LF +G PDFIEI
Sbjct: 473  AIDRPLFGDFWERFLDSLKALKEKEQRTVYRLTLVKGWNAEEVDAYFNLFSIGKPDFIEI 532

Query: 601  KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKF 422
            KGVTYCGSSATSKLTMENVPWHSDVKEFSEALA+KSNG YEVACEHVHSCCVLLAKVDKF
Sbjct: 533  KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAKKSNGGYEVACEHVHSCCVLLAKVDKF 592

Query: 421  KVDGQWYTWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRH 242
            K+DGQWYTWIDY+KFHDLV++G+PFTSKDYMA TP WAVYGAEEGGFDP+Q R++KER H
Sbjct: 593  KIDGQWYTWIDYEKFHDLVASGKPFTSKDYMAPTPSWAVYGAEEGGFDPEQLRYKKERHH 652

Query: 241  KSAR 230
            KS+R
Sbjct: 653  KSSR 656


>ref|XP_021601254.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 ref|XP_021601255.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 ref|XP_021601256.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 gb|OAY22798.1| hypothetical protein MANES_18G027000 [Manihot esculenta]
 gb|OAY22799.1| hypothetical protein MANES_18G027000 [Manihot esculenta]
          Length = 650

 Score =  847 bits (2189), Expect = 0.0
 Identities = 407/536 (75%), Positives = 455/536 (84%)
 Frame = -2

Query: 1840 AFLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELV 1661
            +F  NWLAESADDFRVG+LL+ +CK++VFGVGS +YG+T+NAVA+G S +LR LGA+E+V
Sbjct: 119  SFFGNWLAESADDFRVGSLLLSKCKFSVFGVGSAAYGDTFNAVAKGFSRRLRDLGANEIV 178

Query: 1660 ELGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            E+GEGDVD G ++  F+ W   ++ +LKG   +NG  F N  V                 
Sbjct: 179  EVGEGDVDGGELDLIFEEWSGKVLRILKGGSVQNGTVFSNGCVGGESDNENIEDGFESDN 238

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    GIVDLEDIAGKGPSRRS  +A+ N K+NG     +KEMVTPVIRANLEKQGY
Sbjct: 239  DGNGFAESGIVDLEDIAGKGPSRRSTAVAEVNEKVNG-----QKEMVTPVIRANLEKQGY 293

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 294  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 353

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGK+WQWKMDDPL IV++AID H KMIKQMKGVPGV  E L+EGLSPRHCALSLVGEPI
Sbjct: 354  PVGKNWQWKMDDPLEIVNSAIDQHAKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 413

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN LVDELH+RRISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATKDSLKAIDRPLF
Sbjct: 414  MYPEINKLVDELHQRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPLF 473

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLKAL+EKQQRTVYRLTLVKGWNTEDVDAY  LF +G PDFIEIKGVTYCG
Sbjct: 474  GDFWERFIDSLKALKEKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGQPDFIEIKGVTYCG 533

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            +SATSKLTMENVPWHSDVK FSEALA KS G YEVACEHVHSCCVLLAK +KFKV+GQW+
Sbjct: 534  TSATSKLTMENVPWHSDVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTEKFKVNGQWF 593

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSA 233
            TWIDYDKFHDLV++G+PF  KDYMA TP WAVYGA+EGGFDP QSR+RKER H+++
Sbjct: 594  TWIDYDKFHDLVASGKPFNGKDYMATTPSWAVYGAQEGGFDPDQSRYRKERHHRTS 649


>ref|XP_021667680.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Hevea brasiliensis]
          Length = 650

 Score =  844 bits (2180), Expect = 0.0
 Identities = 405/536 (75%), Positives = 453/536 (84%)
 Frame = -2

Query: 1840 AFLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELV 1661
            +F  NWLA+SADDFRVG+LL+ +CK+AVFGVGS +YG T+NAVA+  S +LR+LGA+E+V
Sbjct: 119  SFFANWLADSADDFRVGSLLLSKCKFAVFGVGSTAYGGTFNAVAKDFSRRLRELGANEIV 178

Query: 1660 ELGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXX 1481
            E+G+GDVD G ++  FD+W   ++ +LKG   +NG  F N                    
Sbjct: 179  EVGKGDVDGGELDGIFDQWSGKVLRILKGGSMQNGLVFSNGCTGGESDNENIEDGFESED 238

Query: 1480 XXXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    GIVDLEDIAGKGPSRRS  +A+ NGK NG     +KEMVTPVIRANLEKQGY
Sbjct: 239  DENGDVESGIVDLEDIAGKGPSRRSNTVAEVNGKANG-----QKEMVTPVIRANLEKQGY 293

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 294  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 353

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGK+WQWKMDDPL IV++AID HTKMIKQMKGVPGV  E L+EGLSPRHCALSLVGEPI
Sbjct: 354  PVGKNWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTQERLTEGLSPRHCALSLVGEPI 413

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN LVDELHRR+ISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATKDSLKAIDRPLF
Sbjct: 414  MYPEINKLVDELHRRQISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPLF 473

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLK+L+EKQQRTVYRLTLVKGWNTEDVDAY  LF +G PDFIEIKGVTYCG
Sbjct: 474  GDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDVDAYFNLFKIGQPDFIEIKGVTYCG 533

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWHSDVK FSEALA KS G YEVACEH HSCCVLLAK +KFKV+GQW+
Sbjct: 534  SSATSKLTMENVPWHSDVKAFSEALALKSKGEYEVACEHAHSCCVLLAKTEKFKVNGQWF 593

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSA 233
            TWIDY+ FHDLV++G+PF S DYMA TP WAVYGA+EGGFDP QSR+RKER H+S+
Sbjct: 594  TWIDYESFHDLVASGKPFNSMDYMAATPSWAVYGAQEGGFDPDQSRYRKERHHRSS 649


>ref|XP_012071843.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 ref|XP_012071844.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 ref|XP_020534805.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 gb|KDP38500.1| hypothetical protein JCGZ_04425 [Jatropha curcas]
          Length = 650

 Score =  842 bits (2174), Expect = 0.0
 Identities = 400/536 (74%), Positives = 454/536 (84%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWLAESADDFRVG+LL+  CK+AVFGVGS++YG+T+NAVA+  S +LR+LGA+E++ 
Sbjct: 120  FFSNWLAESADDFRVGSLLLTNCKFAVFGVGSRAYGDTFNAVAKDFSRRLRELGANEILT 179

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            +GEGDVD G ++  F+ W   +VG+LKG   ENG  F N  +                  
Sbjct: 180  VGEGDVDGGELDGFFEEWSEKVVGILKGGRVENGEVFINGCIGSESDNENIVDEFGSDDD 239

Query: 1477 XXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGYK 1298
                   GIVDLEDIAGKGP+R+   + + NGKLNG     +KEMVTPVIRANLEKQGYK
Sbjct: 240  ENVGVESGIVDLEDIAGKGPTRKFNAVVETNGKLNG-----QKEMVTPVIRANLEKQGYK 294

Query: 1297 VIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 1118
            +IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP
Sbjct: 295  IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 354

Query: 1117 VGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPIM 938
            VGK+WQWKMDDPL IV++AID HTKMIKQMKGVPGV  E L+EGL PRHCALSLVGEPIM
Sbjct: 355  VGKNWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLHPRHCALSLVGEPIM 414

Query: 937  YPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFS 758
            YPEIN+LVDELHRRRISTFLVTNAQFPEKI MLKP+TQLYVSVDAATK+SLK+IDRPLF 
Sbjct: 415  YPEINTLVDELHRRRISTFLVTNAQFPEKITMLKPVTQLYVSVDAATKESLKSIDRPLFG 474

Query: 757  DFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCGS 578
            DFWERF+DSLKAL+EKQQRTVYRLTLVKGWNTED+D Y +LF +G PDFIEIKGVTYCGS
Sbjct: 475  DFWERFIDSLKALKEKQQRTVYRLTLVKGWNTEDIDDYFKLFSIGQPDFIEIKGVTYCGS 534

Query: 577  SATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWYT 398
            SATSKLTMENVPWH+DVK FSEALA KS G YEVACEH HSCCVLLA+ +KFK++GQW+T
Sbjct: 535  SATSKLTMENVPWHADVKAFSEALALKSKGEYEVACEHAHSCCVLLARTEKFKINGQWFT 594

Query: 397  WIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            WIDY+KFHDLV++G+PF SKDY+A TP WAVYGA+EGGFDP QSR+RKER HKS+R
Sbjct: 595  WIDYEKFHDLVASGKPFNSKDYVAATPFWAVYGAQEGGFDPDQSRYRKERHHKSSR 650


>ref|XP_018624471.1| PREDICTED: uncharacterized protein LOC104090695 [Nicotiana
            tomentosiformis]
          Length = 1508

 Score =  870 bits (2249), Expect = 0.0
 Identities = 421/544 (77%), Positives = 468/544 (86%), Gaps = 8/544 (1%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F ++WL ES DDFRVG+LL+ +CK+A+FGVGS SYGET+NAVAR  S KLR+LG  E++E
Sbjct: 962  FFIDWLNESVDDFRVGSLLLSKCKFAIFGVGSGSYGETFNAVARDFSKKLRQLGGVEVLE 1021

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            +GEGDVDEGN+++ F +W + +VGVL  N G+NG +  N GV                  
Sbjct: 1022 VGEGDVDEGNLDEVFGKWSKRVVGVLS-NLGQNGGYMVN-GVGDGNEDEVVSEGDDDDDD 1079

Query: 1477 XXXXXXXG-------IVDLEDIAGKGPSRRSMMLAK-ANGKLNGHVLNGEKEMVTPVIRA 1322
                           I+DLEDIAGKGPSR+  +  K  NGKLNG ++NGEKEMVTPVIRA
Sbjct: 1080 DDDDDEFEENDEGSEILDLEDIAGKGPSRKKGVGDKLVNGKLNGDIVNGEKEMVTPVIRA 1139

Query: 1321 NLEKQGYKVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 1142
            +L KQGYK+IGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF
Sbjct: 1140 SLVKQGYKIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVF 1199

Query: 1141 CWRHHTNPVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCAL 962
            CWRHHTNPVGKSW+WKMDDP+ IVDTAIDLHTKMIKQMKGVPGVKAE LSEGLSPRHCAL
Sbjct: 1200 CWRHHTNPVGKSWRWKMDDPIEIVDTAIDLHTKMIKQMKGVPGVKAERLSEGLSPRHCAL 1259

Query: 961  SLVGEPIMYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLK 782
            SLVGEPIMYPEINSLVDELH+RRISTFLVTNAQFP+KIK+LKPITQLYVSVDA TKDSLK
Sbjct: 1260 SLVGEPIMYPEINSLVDELHKRRISTFLVTNAQFPDKIKLLKPITQLYVSVDAGTKDSLK 1319

Query: 781  AIDRPLFSDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEI 602
            AIDRPLF DFWERF+DSLKAL+EK+QRTVYRLTLVKGWN E+VDAY  LF +G PDFIEI
Sbjct: 1320 AIDRPLFGDFWERFLDSLKALKEKEQRTVYRLTLVKGWNAEEVDAYFSLFSIGKPDFIEI 1379

Query: 601  KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKF 422
            KGVTYCGSSATSKLTMENVPWHSDVKEFSEALA+KSNG YEVACEHVHSCCVLLAKVDKF
Sbjct: 1380 KGVTYCGSSATSKLTMENVPWHSDVKEFSEALAKKSNGGYEVACEHVHSCCVLLAKVDKF 1439

Query: 421  KVDGQWYTWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRH 242
            K+DGQWYTWIDY+KFHDLV++G+PFTSKDYMA TP WAVYGAEEGGFDP+Q R++KER H
Sbjct: 1440 KIDGQWYTWIDYEKFHDLVASGKPFTSKDYMAPTPSWAVYGAEEGGFDPEQLRYKKERHH 1499

Query: 241  KSAR 230
            KS+R
Sbjct: 1500 KSSR 1503


>ref|NP_001347568.1| uncharacterized protein LOC111946220 [Ricinus communis]
          Length = 642

 Score =  835 bits (2156), Expect = 0.0
 Identities = 406/539 (75%), Positives = 452/539 (83%), Gaps = 4/539 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWLAESADDFRVG+LL+  CK+AVFGVGS++YG+++NA A+  S +LR+LGA+E+V 
Sbjct: 114  FFANWLAESADDFRVGSLLLSNCKFAVFGVGSRAYGDSFNAAAKDFSKRLRELGANEIVA 173

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGW---HFENYGVXXXXXXXXXXXXXXX 1487
            +GEGDVD G ++  F+ W   +VGVLKG   ENG+     EN  V               
Sbjct: 174  IGEGDVDSGEIDAAFEEWSGKVVGVLKGGSLENGFIGCESENDNVVVDDDDFQSSDEENG 233

Query: 1486 XXXXXXXXXXGIVDLEDIAGKGPSRRSM-MLAKANGKLNGHVLNGEKEMVTPVIRANLEK 1310
                       IVDLEDIAGKGPSRR    + + N K NG     +KEMVTPVIRA+LEK
Sbjct: 234  ELESG------IVDLEDIAGKGPSRRRFNAVTETNSKPNG-----QKEMVTPVIRASLEK 282

Query: 1309 QGYKVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH 1130
            QGYK+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH
Sbjct: 283  QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH 342

Query: 1129 HTNPVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVG 950
            HTNPVGK+WQWKMDDPL IV++AID HTKMIKQMKGVPGV  E L+EGLSPRHCALSLVG
Sbjct: 343  HTNPVGKNWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTMERLNEGLSPRHCALSLVG 402

Query: 949  EPIMYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDR 770
            EPIMYPEIN+LV+ELH+RRISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATK+SLKAIDR
Sbjct: 403  EPIMYPEINTLVNELHQRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKESLKAIDR 462

Query: 769  PLFSDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVT 590
            PLF DFWERF+DSLKALREKQQRTVYRLTLVKGWNTEDVDAY  LF +G PDFIEIKGVT
Sbjct: 463  PLFGDFWERFIDSLKALREKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGQPDFIEIKGVT 522

Query: 589  YCGSSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDG 410
            YCGSSATSKLTMENVPWHSDVK FSEALA KS G YEVACEHVHSCCVLLAK +KFKV+G
Sbjct: 523  YCGSSATSKLTMENVPWHSDVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTEKFKVNG 582

Query: 409  QWYTWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSA 233
            QW+TWIDY+KFH+LV++G+PF SKDYMA TP WAVYGA EGGFDP QSR+RKER HKS+
Sbjct: 583  QWFTWIDYEKFHNLVASGKPFNSKDYMAPTPSWAVYGATEGGFDPDQSRYRKERHHKSS 641


>ref|XP_022772746.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Durio zibethinus]
          Length = 631

 Score =  834 bits (2154), Expect = 0.0
 Identities = 407/537 (75%), Positives = 450/537 (83%), Gaps = 1/537 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F VNWLAE + DFR G LL+ +CK+AVFGVGS+ YGET+NA+AR +  +L+ LGA+E+V 
Sbjct: 108  FFVNWLAEISTDFRAGNLLLSDCKFAVFGVGSRVYGETFNALARDLGKRLKGLGATEMVA 167

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            +GEGDVD G ++  F+ W   +V VLKG     G   EN  V                  
Sbjct: 168  VGEGDVDGGALDSVFEEWSEKVVTVLKG-----GLVVENENV---IVYESDVESLESSDD 219

Query: 1477 XXXXXXXGIVDLEDIAGKGPSR-RSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    IVDLEDIAGKGPSR +++ +A+ NGK     L+G++EMVTPV+RANLEKQGY
Sbjct: 220  DGAGGGEEIVDLEDIAGKGPSRKKAVNVAETNGK-----LDGKREMVTPVMRANLEKQGY 274

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN
Sbjct: 275  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 334

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSWQWKMDDPL IV+TAIDLHT MIKQMKGVPGV  E L EGLSPRHCALSLVGEPI
Sbjct: 335  PVGKSWQWKMDDPLEIVNTAIDLHTNMIKQMKGVPGVTQERLLEGLSPRHCALSLVGEPI 394

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN LVDELHRR+ISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATKDSLKAIDRPLF
Sbjct: 395  MYPEINMLVDELHRRQISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPLF 454

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLKAL+EK QRTVYRLTLVKGWNTEDVDAYS+LF +G PDF+EIKGVTYCG
Sbjct: 455  GDFWERFIDSLKALKEKHQRTVYRLTLVKGWNTEDVDAYSKLFSIGKPDFVEIKGVTYCG 514

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWHSDVK FSEALAQKS G YEVACEH HSCCVLLAK +KFKV+GQWY
Sbjct: 515  SSATSKLTMENVPWHSDVKSFSEALAQKSEGQYEVACEHAHSCCVLLAKTEKFKVNGQWY 574

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            TWIDY+KFHDLV++G PF SKDYMA TP WAVYGAEEGGFDP+QSR+RKER HKS R
Sbjct: 575  TWIDYEKFHDLVASGRPFDSKDYMAFTPSWAVYGAEEGGFDPEQSRYRKERHHKSDR 631


>ref|XP_015884714.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Ziziphus jujuba]
          Length = 647

 Score =  834 bits (2155), Expect = 0.0
 Identities = 402/536 (75%), Positives = 455/536 (84%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWL+ES+DDFRVG+LL+ +CK+AVFGVGS++YG T+NAVA+ +S ++R LGA E++ 
Sbjct: 120  FFANWLSESSDDFRVGSLLLSQCKFAVFGVGSRAYGATFNAVAKDVSKQMRALGAKEILP 179

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            + EGDVD G++++ FD W   +VG  KG   ENG    N GV                  
Sbjct: 180  VWEGDVDGGDIDEVFDAWSGKVVGFCKGGVVENGGVSTN-GVDCESDACSGDETDEEEYS 238

Query: 1477 XXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGYK 1298
                    IVDLEDIAGKGPSRRS  + KA  + NG  LNG+K+MVTPVIRA+LEKQGYK
Sbjct: 239  MESE----IVDLEDIAGKGPSRRSATVTKA--ETNG-TLNGKKDMVTPVIRASLEKQGYK 291

Query: 1297 VIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 1118
            +IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP
Sbjct: 292  IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 351

Query: 1117 VGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPIM 938
            VGKSWQWKMDDPL IV++AI+ HTKMIKQMKGVPGV  E L EGLSPRHCALSLVGEPIM
Sbjct: 352  VGKSWQWKMDDPLEIVNSAIEQHTKMIKQMKGVPGVTVERLREGLSPRHCALSLVGEPIM 411

Query: 937  YPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFS 758
            YPEIN+LVDELHRRR+STFLVTNAQFPEKIKMLKPITQLYVSVDAATK+SLKAIDRPLF 
Sbjct: 412  YPEINTLVDELHRRRVSTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLFG 471

Query: 757  DFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCGS 578
            DFWER +DSLKAL+EKQQRTVYRLTLVKGWNTED++AYS LF +G PDFIEIKGVTYCGS
Sbjct: 472  DFWERLIDSLKALKEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGKPDFIEIKGVTYCGS 531

Query: 577  SATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWYT 398
            SATSKLTMENVPWHSDV++FSEALA +S G YEVACEHVHSCCVLLAK DKFK++GQW+T
Sbjct: 532  SATSKLTMENVPWHSDVRDFSEALALRSLGEYEVACEHVHSCCVLLAKTDKFKINGQWFT 591

Query: 397  WIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            WIDY+KFHDLV++G PF SKDYMA TP WAVYGAEEGGFDP QSR++KER H+S++
Sbjct: 592  WIDYEKFHDLVASGRPFDSKDYMAITPSWAVYGAEEGGFDPDQSRYKKERHHRSSK 647


>ref|XP_020410607.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Prunus persica]
 gb|ONI32680.1| hypothetical protein PRUPE_1G379500 [Prunus persica]
 gb|ONI32681.1| hypothetical protein PRUPE_1G379500 [Prunus persica]
          Length = 643

 Score =  832 bits (2150), Expect = 0.0
 Identities = 399/535 (74%), Positives = 451/535 (84%), Gaps = 1/535 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWLAESA+DFRVG+LL+  CK+AVFGVGS++YG T+NAV + ++ ++R LGASE+  
Sbjct: 114  FFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAKRMRALGASEIFS 173

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            + EGDVD G++++ F+ W   ++  LKG   ENG    N GV                  
Sbjct: 174  IWEGDVDGGDIDEVFEAWSGKVLRFLKGGVAENGGVLSN-GV-GAECEAESFDGSDEDEE 231

Query: 1477 XXXXXXXGIVDLEDIAGKGPS-RRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    +VDLEDIAGKGPS RR++ + K NG      +NG+KEMVTPVIRA+L KQGY
Sbjct: 232  EEDDVESEVVDLEDIAGKGPSRRRTVTVTKTNG-----TVNGQKEMVTPVIRASLVKQGY 286

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 287  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 346

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSWQWKMDDPL IV++AIDLHTKMIKQMKGVPGV  E L+EGLSPRHCALSLVGEPI
Sbjct: 347  PVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 406

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN+LVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATK+SLKAIDRPLF
Sbjct: 407  MYPEINALVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLF 466

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLK+L+EKQQRTVYRLTLVKGWNTED+DAY  +F +G+PDF+EIKGVTYCG
Sbjct: 467  GDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDIDAYYNIFSIGNPDFVEIKGVTYCG 526

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWH+DVK FSEALA KSNG YEVACEHVHSCCVLLAK DKFK++GQW+
Sbjct: 527  SSATSKLTMENVPWHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTDKFKINGQWF 586

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKS 236
            TWIDY+KFHDLVS+G PF SKDYMA TP WAVYGAEEGGFDP QSR++KER H+S
Sbjct: 587  TWIDYEKFHDLVSSGRPFDSKDYMAATPSWAVYGAEEGGFDPYQSRYKKERHHRS 641


>ref|XP_021900738.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Carica papaya]
          Length = 651

 Score =  832 bits (2149), Expect = 0.0
 Identities = 401/544 (73%), Positives = 455/544 (83%), Gaps = 10/544 (1%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            FL NWL+ESADDFRVG+LL+ +CK+AVFGVGS++YG+TYNAVAR +S ++R+LG  E+  
Sbjct: 122  FLANWLSESADDFRVGSLLLSKCKFAVFGVGSRAYGDTYNAVARDLSKRVRELGGVEIFP 181

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFG-ENG-------WHFENYGVXXXXXXXXXX 1502
            +GEGDVD+G ++  FD W R +V VLKG    ENG       W  +   +          
Sbjct: 182  VGEGDVDDGELDIVFDEWSRKVVSVLKGTLVLENGAVLGNGIWEIDGESI---------- 231

Query: 1501 XXXXXXXXXXXXXXXGIVDLEDIAGKGPSRR--SMMLAKANGKLNGHVLNGEKEMVTPVI 1328
                            IVD+EDIAGK PSR+  +  +A+ NGK     ++G++ MVTPVI
Sbjct: 232  -EDSGDDDEQSLVETEIVDVEDIAGKAPSRKMATANVAETNGK-----IDGKRAMVTPVI 285

Query: 1327 RANLEKQGYKVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKC 1148
            RA+LEKQGYK+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKC
Sbjct: 286  RASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKC 345

Query: 1147 VFCWRHHTNPVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHC 968
            VFCWRHHTNPVGKSWQWKMDDPL IV++AIDLHTKMIKQMKGVPGV  E L+EGLSPRHC
Sbjct: 346  VFCWRHHTNPVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTMERLTEGLSPRHC 405

Query: 967  ALSLVGEPIMYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDS 788
            ALSLVGEPIMYPEIN+LVDELHRRRISTFLVTNAQFPEKIKMLKP+TQLYVSVDAATK+S
Sbjct: 406  ALSLVGEPIMYPEINALVDELHRRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKES 465

Query: 787  LKAIDRPLFSDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFI 608
            LKA+DRPLF DFWERF+DSLKALREK+QRTVYRLTLVKGWNTE++DAY  LF +G PDF+
Sbjct: 466  LKAVDRPLFGDFWERFIDSLKALREKEQRTVYRLTLVKGWNTEEIDAYFNLFSIGKPDFV 525

Query: 607  EIKGVTYCGSSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVD 428
            EIKGVTYCGSSATSKLTMENVPWHSDVK FSEALA KS G YEVACEH HSCCVLLAK D
Sbjct: 526  EIKGVTYCGSSATSKLTMENVPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTD 585

Query: 427  KFKVDGQWYTWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKER 248
            KFKV+ QW+TWIDY+KFHDLV++G PF+S+DYMA TP WAVYGAEEGGFDP QSR++KER
Sbjct: 586  KFKVNNQWFTWIDYEKFHDLVASGRPFSSRDYMAATPSWAVYGAEEGGFDPDQSRYKKER 645

Query: 247  RHKS 236
            RHKS
Sbjct: 646  RHKS 649


>ref|XP_008220799.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Prunus mume]
          Length = 643

 Score =  831 bits (2147), Expect = 0.0
 Identities = 398/535 (74%), Positives = 451/535 (84%), Gaps = 1/535 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWLAESA+DFRVG+LL+  CK+AVFGVGS++YG T+NAV + ++ ++R LGA+E+  
Sbjct: 114  FFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAKRMRALGAAEIFS 173

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            + EGDVD G++++ F+ W   ++  LKG   ENG    N GV                  
Sbjct: 174  IWEGDVDGGDIDEVFEAWSGKVLRFLKGGVAENGGVLSN-GV-GAECEAESFDGSDEDDE 231

Query: 1477 XXXXXXXGIVDLEDIAGKGPS-RRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    +VDLEDIAGKGPS RR++ + K NG      +NG+KEMVTPVIRA+L KQGY
Sbjct: 232  EEDDLESEVVDLEDIAGKGPSRRRTVTVTKTNG-----TVNGQKEMVTPVIRASLVKQGY 286

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 287  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 346

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSWQWKMDDPL IV++AIDLHTKMIKQMKGVPGV  E L+EGLSPRHCALSLVGEPI
Sbjct: 347  PVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 406

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN+LVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATK+SLKAIDRPLF
Sbjct: 407  MYPEINALVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLF 466

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLK+L+EKQQRTVYRLTLVKGWNTED+DAY  +F +G+PDF+EIKGVTYCG
Sbjct: 467  GDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDIDAYYNIFSIGNPDFVEIKGVTYCG 526

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWH+DVK FSEALA KSNG YEVACEHVHSCCVLLAK DKFK++GQW+
Sbjct: 527  SSATSKLTMENVPWHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTDKFKINGQWF 586

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKS 236
            TWIDY+KFHDLVS+G PF SKDYMA TP WAVYGAEEGGFDP QSR++KER H+S
Sbjct: 587  TWIDYEKFHDLVSSGRPFDSKDYMAATPSWAVYGAEEGGFDPYQSRYKKERHHRS 641


>ref|XP_021826483.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Prunus avium]
          Length = 643

 Score =  828 bits (2138), Expect = 0.0
 Identities = 397/535 (74%), Positives = 449/535 (83%), Gaps = 1/535 (0%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  NWLAESA+DFRVG+LL+  CK+AVFGVGS++YG T+NAV + ++ ++R LGA E+  
Sbjct: 114  FFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAKRMRALGAYEIFS 173

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            + EGDVD G++++ F+ W   ++  LKG   ENG    N GV                  
Sbjct: 174  IWEGDVDGGDIDEVFEAWSGKVLRFLKGGVAENGGVLSN-GV-GAECEAESFDGSDEDDE 231

Query: 1477 XXXXXXXGIVDLEDIAGKGPS-RRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGY 1301
                    +VDLEDIAGKGPS RR++ + K NG      +NG+KEMVTPVIRA+L KQGY
Sbjct: 232  EEDDVESEVVDLEDIAGKGPSRRRTVTVTKTNG-----TVNGQKEMVTPVIRASLVKQGY 286

Query: 1300 KVIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1121
            K+IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 287  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 346

Query: 1120 PVGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPI 941
            PVGKSWQWKMDDPL IV++AID HTKMIKQMKGVPGV  E L+EGLSPRHCALSLVGEPI
Sbjct: 347  PVGKSWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 406

Query: 940  MYPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLF 761
            MYPEIN+LVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATK+SLKAIDRPLF
Sbjct: 407  MYPEINALVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLF 466

Query: 760  SDFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCG 581
             DFWERF+DSLK+L+EKQQRTVYRLTLVKGWNTED+DAY  +F +G+PDF+EIKGVTYCG
Sbjct: 467  GDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDIDAYYNIFSIGNPDFVEIKGVTYCG 526

Query: 580  SSATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWY 401
            SSATSKLTMENVPWH+DVK FSEALA KSNG YEVACEHVHSCCVLLAK DKFK++GQW+
Sbjct: 527  SSATSKLTMENVPWHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTDKFKINGQWF 586

Query: 400  TWIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKS 236
            TWIDY+KFHDLVS+G PF SKDYMA TP WAVYGAEEGGFDP QSR++KER H+S
Sbjct: 587  TWIDYEKFHDLVSSGRPFDSKDYMAATPSWAVYGAEEGGFDPYQSRYKKERHHRS 641


>ref|XP_018821629.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Juglans regia]
          Length = 648

 Score =  827 bits (2137), Expect = 0.0
 Identities = 395/536 (73%), Positives = 448/536 (83%)
 Frame = -2

Query: 1837 FLVNWLAESADDFRVGALLMKECKYAVFGVGSKSYGETYNAVARGISVKLRKLGASELVE 1658
            F  +WL+ESA+DFRVG+LL+  CK+AVFGVGS +YG T+NAVAR  S ++R LGA+E++ 
Sbjct: 118  FFSSWLSESAEDFRVGSLLLSRCKFAVFGVGSGAYGATFNAVARDYSKQMRALGANEILP 177

Query: 1657 LGEGDVDEGNVNDEFDRWCRNIVGVLKGNFGENGWHFENYGVXXXXXXXXXXXXXXXXXX 1478
            + EGDVD G+ ++ F+ W   +VG+LK    +NG    +                     
Sbjct: 178  VWEGDVDNGDFDEVFEAWSAKVVGLLKVGVLDNGAVLRSETEALAGGVSDAESFGSSDED 237

Query: 1477 XXXXXXXGIVDLEDIAGKGPSRRSMMLAKANGKLNGHVLNGEKEMVTPVIRANLEKQGYK 1298
                    IVDLEDIAGKGPSR+S ++A++NGKLNG     +KEMVTPVIRA+LEKQGYK
Sbjct: 238  EDREGSAEIVDLEDIAGKGPSRKSTVMAESNGKLNG-----KKEMVTPVIRASLEKQGYK 292

Query: 1297 VIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 1118
            +IGSHSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP
Sbjct: 293  IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 352

Query: 1117 VGKSWQWKMDDPLVIVDTAIDLHTKMIKQMKGVPGVKAELLSEGLSPRHCALSLVGEPIM 938
            VGKSWQWKMDDPL IV+TAIDLHT MIKQMKGVPGVK E L EGL+PRHCALSLVGEPIM
Sbjct: 353  VGKSWQWKMDDPLEIVNTAIDLHTNMIKQMKGVPGVKLERLMEGLAPRHCALSLVGEPIM 412

Query: 937  YPEINSLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFS 758
            YPEIN+LVDELHRRRISTFLVTNAQFPEKI+ LKP+TQLYVSVDA TK+SLKAIDRPLF 
Sbjct: 413  YPEINTLVDELHRRRISTFLVTNAQFPEKIEALKPVTQLYVSVDAGTKESLKAIDRPLFG 472

Query: 757  DFWERFVDSLKALREKQQRTVYRLTLVKGWNTEDVDAYSRLFDVGDPDFIEIKGVTYCGS 578
            DFWERF+DSLKAL+EKQQRTVYRLTLVKGWN ED+DAYS LF +G PDF+EIKGVTYCGS
Sbjct: 473  DFWERFIDSLKALKEKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGKPDFVEIKGVTYCGS 532

Query: 577  SATSKLTMENVPWHSDVKEFSEALAQKSNGAYEVACEHVHSCCVLLAKVDKFKVDGQWYT 398
            SATSKLTMENVPWHSDVK FSE LA +S G YEVACEHVHSCCVLLA  +KFKV+G+W+T
Sbjct: 533  SATSKLTMENVPWHSDVKAFSETLALRSQGEYEVACEHVHSCCVLLANTEKFKVNGKWFT 592

Query: 397  WIDYDKFHDLVSAGEPFTSKDYMAETPLWAVYGAEEGGFDPQQSRFRKERRHKSAR 230
            WIDY+KFHDLV++G+PF S DYMA TP WAVYGAEEGGFDP QSR++KER HKS R
Sbjct: 593  WIDYEKFHDLVASGKPFDSMDYMAATPSWAVYGAEEGGFDPDQSRYKKERHHKSGR 648