BLASTX nr result
ID: Rehmannia28_contig00022623
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia28_contig00022623
(1175 letters)
Database: ./nr
84,704,028 sequences; 31,038,470,784 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011089495.1| PREDICTED: uncharacterized protein LOC105170... 464 e-160
ref|XP_012858369.1| PREDICTED: uncharacterized protein LOC105977... 439 e-150
ref|XP_009613423.1| PREDICTED: uncharacterized protein LOC104106... 432 e-147
emb|CDP09083.1| unnamed protein product [Coffea canephora] 431 e-147
ref|XP_006359488.1| PREDICTED: uncharacterized protein LOC102605... 427 e-145
ref|XP_009796388.1| PREDICTED: uncharacterized protein LOC104242... 427 e-145
ref|XP_015082709.1| PREDICTED: uncharacterized protein LOC107026... 426 e-145
ref|XP_004242746.1| PREDICTED: uncharacterized protein LOC101262... 426 e-145
ref|XP_002280733.1| PREDICTED: uncharacterized protein LOC100255... 422 e-143
ref|XP_012066994.1| PREDICTED: uncharacterized protein LOC105629... 412 e-140
ref|XP_011035283.1| PREDICTED: uncharacterized protein LOC105133... 411 e-139
ref|XP_010262021.1| PREDICTED: uncharacterized protein LOC104600... 410 e-139
ref|XP_008356928.1| PREDICTED: uncharacterized protein LOC103420... 409 e-138
ref|XP_004294649.1| PREDICTED: uncharacterized protein LOC101293... 409 e-138
ref|XP_002314167.1| hypothetical protein POPTR_0009s03890g [Popu... 409 e-138
gb|KNA17899.1| hypothetical protein SOVF_075600 [Spinacia oleracea] 408 e-138
ref|XP_010043508.1| PREDICTED: uncharacterized protein LOC104432... 408 e-138
ref|XP_002299861.2| hypothetical protein POPTR_0001s24920g [Popu... 407 e-137
ref|XP_007016369.1| C-terminal S-isoprenylcysteine carboxyl O-me... 406 e-137
ref|XP_009364365.1| PREDICTED: uncharacterized protein LOC103954... 406 e-137
>ref|XP_011089495.1| PREDICTED: uncharacterized protein LOC105170433 [Sesamum indicum]
Length = 418
Score = 464 bits (1194), Expect = e-160
Identities = 215/254 (84%), Positives = 230/254 (90%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPWM I LAIYGVY L+KYLKGEA+L EQLAIVTSAFTWLTLVPPAHFN
Sbjct: 165 WMYFSWTWLFWPWMAVIALAIYGVYCLNKYLKGEASLFEQLAIVTSAFTWLTLVPPAHFN 224
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVSVRKRLYGDY+PREHD+KWDISLPNW+K+LF VGVMVG
Sbjct: 225 GFLEGWPFVFFFVYHYFFFLNVSVRKRLYGDYFPREHDSKWDISLPNWHKVLFSVGVMVG 284
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAA EGTELHL+PGGWNNVGIW++IML VFMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 285 HWLAALEGTELHLIPGGWNNVGIWIMIMLAVFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 344
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTYFTALRAPMS+LF + VCL+YY QKAKLEEALMLETFGERYSE
Sbjct: 345 RFVRHPIYASTMLLFVTYFTALRAPMSSLFIILVCLVYYAQKAKLEEALMLETFGERYSE 404
Query: 721 YMSKVRYKLIPFVY 762
YM+KV YKLIPFVY
Sbjct: 405 YMNKVHYKLIPFVY 418
Score = 59.7 bits (143), Expect = 3e-06
Identities = 32/56 (57%), Positives = 42/56 (75%), Gaps = 1/56 (1%)
Frame = +3
Query: 864 ISYFIALGACVPVVYSCGLLDVLWT-KAELEEALMLETFGEGYNEYMSKVHYKLIP 1028
++YF AL A + ++ + V + KA+LEEALMLETFGE Y+EYM+KVHYKLIP
Sbjct: 360 VTYFTALRAPMSSLFIILVCLVYYAQKAKLEEALMLETFGERYSEYMNKVHYKLIP 415
>ref|XP_012858369.1| PREDICTED: uncharacterized protein LOC105977593 [Erythranthe
guttata] gi|604300247|gb|EYU20090.1| hypothetical
protein MIMGU_mgv1a007401mg [Erythranthe guttata]
Length = 409
Score = 439 bits (1128), Expect = e-150
Identities = 201/254 (79%), Positives = 221/254 (87%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
W+YFSWTWLFWPW+VAI LAIYG++SL+K+L GEA L EQLAI+TS+FTWLTLVPPAHFN
Sbjct: 156 WLYFSWTWLFWPWIVAIALAIYGIFSLTKHLNGEAKLHEQLAIITSSFTWLTLVPPAHFN 215
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNV+VRKR+YGDY PR HD KWDIS P W+K+LFC G+ +G
Sbjct: 216 GFLEGWPFVFFFIYHYFFFLNVTVRKRMYGDYNPRAHDPKWDISSPGWHKILFCAGIFLG 275
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEGTELHLVPGGW NVGIW LI++TVFMQYHSTLYL+KYSE VVVPTAVVQFGPY
Sbjct: 276 HWLAAFEGTELHLVPGGWANVGIWALILVTVFMQYHSTLYLAKYSENVVVPTAVVQFGPY 335
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTYF ALRAP S LF V VC YYGQKAK+EEALMLE+FGERYSE
Sbjct: 336 RFVRHPIYASTMLLFVTYFAALRAPFSLLFIVGVCSAYYGQKAKMEEALMLESFGERYSE 395
Query: 721 YMSKVRYKLIPFVY 762
YMSKVRYKLIPFVY
Sbjct: 396 YMSKVRYKLIPFVY 409
Score = 59.3 bits (142), Expect = 4e-06
Identities = 30/56 (53%), Positives = 41/56 (73%), Gaps = 1/56 (1%)
Frame = +3
Query: 864 ISYFIALGACVPVVYSCGLLDVLW-TKAELEEALMLETFGEGYNEYMSKVHYKLIP 1028
++YF AL A +++ G+ + KA++EEALMLE+FGE Y+EYMSKV YKLIP
Sbjct: 351 VTYFAALRAPFSLLFIVGVCSAYYGQKAKMEEALMLESFGERYSEYMSKVRYKLIP 406
>ref|XP_009613423.1| PREDICTED: uncharacterized protein LOC104106558 [Nicotiana
tomentosiformis]
Length = 402
Score = 432 bits (1111), Expect = e-147
Identities = 200/254 (78%), Positives = 220/254 (86%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFS TWLFWPW+VAI LA Y +Y L+K+L GEA+ +EQ AIVTSAFTWLTLVPPAHFN
Sbjct: 149 WMYFSMTWLFWPWLVAISLASYSLYCLNKHLNGEASELEQFAIVTSAFTWLTLVPPAHFN 208
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVSVRKRLYGDY PREHD KWDISLPNW KLLFCVGVMVG
Sbjct: 209 GFLEGWPVVFFFVYHYFFFLNVSVRKRLYGDYNPREHDPKWDISLPNWKKLLFCVGVMVG 268
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGW+N+GIWVLI++T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 269 HWLAAFEGPELHLIPGGWSNLGIWVLILMTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 328
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFV Y ALRAP+S+LF VC +YYG KAKLEE+LM+E FGERY+E
Sbjct: 329 RFVRHPIYASTMLLFVAYCVALRAPLSSLFIAVVCSLYYGNKAKLEESLMVENFGERYTE 388
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYKLIPFVY
Sbjct: 389 YASKVRYKLIPFVY 402
>emb|CDP09083.1| unnamed protein product [Coffea canephora]
Length = 403
Score = 431 bits (1109), Expect = e-147
Identities = 195/254 (76%), Positives = 219/254 (86%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPWMVAI LAIYG+Y +++L G AN +QL+IVT+AFTWLTLVPPAHFN
Sbjct: 150 WMYFSWTWLFWPWMVAISLAIYGLYCFNRHLHGNANAFQQLSIVTAAFTWLTLVPPAHFN 209
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVSVRKRLYGDYYPREHD WDI+LP W KLLFCVGVMVG
Sbjct: 210 GFLEGWPIVFFFVYHYFFFFNVSVRKRLYGDYYPREHDPAWDITLPRWQKLLFCVGVMVG 269
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HW AAFEG ELHLVPGGW+N+GIW+LIMLT+FMQYHSTLYL+KYSEKVVVPT+VV FGPY
Sbjct: 270 HWFAAFEGPELHLVPGGWSNLGIWILIMLTLFMQYHSTLYLAKYSEKVVVPTSVVLFGPY 329
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTYF A+RAP+SALF VAVC+ YYGQKAKLEE +M++TFG+ Y+
Sbjct: 330 RFVRHPIYASTMLLFVTYFAAMRAPLSALFIVAVCIFYYGQKAKLEELMMVKTFGQMYTG 389
Query: 721 YMSKVRYKLIPFVY 762
Y +KVRYK IPFVY
Sbjct: 390 YTNKVRYKFIPFVY 403
>ref|XP_006359488.1| PREDICTED: uncharacterized protein LOC102605130 [Solanum tuberosum]
gi|971572346|ref|XP_015169933.1| PREDICTED:
uncharacterized protein LOC102605130 [Solanum tuberosum]
Length = 400
Score = 427 bits (1097), Expect = e-145
Identities = 194/254 (76%), Positives = 220/254 (86%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFS TWL+WPW+VAI LA Y +Y L+K+L+G+AN+ EQ A+VTSAFTWLTLVPPAHFN
Sbjct: 147 WMYFSMTWLYWPWLVAISLASYSLYCLNKHLRGDANVFEQFAVVTSAFTWLTLVPPAHFN 206
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVS+RKRLYGDY PREHD +WDISLP+W KLLFCVGVMVG
Sbjct: 207 GFLEGWPVVFFLVYHYFFFLNVSIRKRLYGDYNPREHDPRWDISLPDWQKLLFCVGVMVG 266
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGW+N+GIW LI++T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 267 HWLAAFEGPELHLIPGGWSNLGIWGLIVMTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 326
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTY ALRAP+SALF VC +YYG KAKLEE+LM+E FGERY E
Sbjct: 327 RFIRHPIYASTMLLFVTYCIALRAPLSALFIAVVCSVYYGNKAKLEESLMVENFGERYME 386
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYKLIP++Y
Sbjct: 387 YASKVRYKLIPYIY 400
>ref|XP_009796388.1| PREDICTED: uncharacterized protein LOC104242973 [Nicotiana
sylvestris]
Length = 402
Score = 427 bits (1097), Expect = e-145
Identities = 200/254 (78%), Positives = 218/254 (85%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFS TWLFWP +VAI LA Y +Y L+K L GEA+ +EQ AIVTSAFTWLTLVPPAHFN
Sbjct: 149 WMYFSMTWLFWPRLVAISLASYSLYCLNKLLNGEASELEQFAIVTSAFTWLTLVPPAHFN 208
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVSVRKRLYGDY PREHD KWDISLPNW KLLFCVGVMVG
Sbjct: 209 GFLEGWPVVFFFVYHYFFFLNVSVRKRLYGDYNPREHDPKWDISLPNWKKLLFCVGVMVG 268
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGW+N+GIWVLI++T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 269 HWLAAFEGPELHLIPGGWSNLGIWVLILMTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 328
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFV Y ALRAP+SALF VCL+YYG KAKLEE+LM+E FGERY E
Sbjct: 329 RFVRHPIYASTMLLFVAYCVALRAPLSALFIAVVCLLYYGNKAKLEESLMIENFGERYME 388
Query: 721 YMSKVRYKLIPFVY 762
Y SKVR+KLIPFVY
Sbjct: 389 YASKVRHKLIPFVY 402
>ref|XP_015082709.1| PREDICTED: uncharacterized protein LOC107026314 [Solanum pennellii]
Length = 400
Score = 426 bits (1095), Expect = e-145
Identities = 194/254 (76%), Positives = 216/254 (85%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFS TWL+WPW+VAI LA Y +Y L+K+L+GEAN+ EQ A+VTS FTWLTLVPPAHFN
Sbjct: 147 WMYFSMTWLYWPWLVAISLASYSLYCLNKHLRGEANVFEQFAVVTSVFTWLTLVPPAHFN 206
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVS+RKRLYGDY PREHD KWDISLPNW KLLFCVGVM G
Sbjct: 207 GFLEGWPVVFFLVYHYFFFLNVSIRKRLYGDYNPREHDPKWDISLPNWQKLLFCVGVMAG 266
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HW AAFEG ELHL+PGGW+N+GIW LI +T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 267 HWFAAFEGPELHLIPGGWSNMGIWGLIAMTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 326
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTY ALRAP+SALF VC +YYG KAKLEE+LM+E FGERY E
Sbjct: 327 RFIRHPIYASTMLLFVTYCIALRAPLSALFIAVVCSVYYGNKAKLEESLMIENFGERYME 386
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYKLIP++Y
Sbjct: 387 YASKVRYKLIPYIY 400
>ref|XP_004242746.1| PREDICTED: uncharacterized protein LOC101262629 [Solanum
lycopersicum]
Length = 400
Score = 426 bits (1095), Expect = e-145
Identities = 194/254 (76%), Positives = 216/254 (85%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFS TWL+WPW+VAI LA Y +Y L+K+L+GEAN+ EQ A+VTS FTWLTLVPPAHFN
Sbjct: 147 WMYFSMTWLYWPWLVAISLASYSLYCLNKHLRGEANVFEQFAVVTSVFTWLTLVPPAHFN 206
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP LNVS+RKRLYGDY PREHD KWDISLPNW KLLFCVGVM G
Sbjct: 207 GFLEGWPVVFFLVYHYFFFLNVSIRKRLYGDYNPREHDPKWDISLPNWQKLLFCVGVMAG 266
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HW AAFEG ELHL+PGGW+N+GIW LI +T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 267 HWFAAFEGPELHLIPGGWSNLGIWGLIAMTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 326
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
RF+RHPIYASTMLLFVTY ALRAP+SALF VC +YYG KAKLEE+LM+E FGERY E
Sbjct: 327 RFIRHPIYASTMLLFVTYCIALRAPLSALFIAVVCSVYYGNKAKLEESLMIENFGERYME 386
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYKLIP++Y
Sbjct: 387 YASKVRYKLIPYIY 400
>ref|XP_002280733.1| PREDICTED: uncharacterized protein LOC100255385 [Vitis vinifera]
Length = 396
Score = 422 bits (1084), Expect = e-143
Identities = 188/254 (74%), Positives = 219/254 (86%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW +A+G+A+YG+Y L ++L GEAN+ EQ+AIVTS FTWLTLVPPAHFN
Sbjct: 143 WMYFSWTWLFWPWFLAVGVAVYGLYCLRRHLCGEANVFEQIAIVTSVFTWLTLVPPAHFN 202
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVSVRKRLYGDY+ R HD KWD+S PNW++LLFC+GVMVG
Sbjct: 203 GFLEGWPFVFFFVYHYFFFFNVSVRKRLYGDYFLRPHDPKWDVSPPNWHRLLFCIGVMVG 262
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAA+EG ELHL+PGGWNN+ +W LIM+T+FMQYHSTLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 263 HWLAAYEGPELHLIPGGWNNLWVWALIMVTLFMQYHSTLYLAKYSEKVVVPTAVVQFGPY 322
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLFV+Y ALRAP+S+LF +AVCLMYY QK KLEEA M+ETFGE+Y E
Sbjct: 323 RWVRHPIYASTMLLFVSYCLALRAPISSLFVIAVCLMYYEQKVKLEEASMVETFGEKYME 382
Query: 721 YMSKVRYKLIPFVY 762
Y +KV+YK IPFVY
Sbjct: 383 YANKVKYKFIPFVY 396
>ref|XP_012066994.1| PREDICTED: uncharacterized protein LOC105629944 [Jatropha curcas]
gi|643735708|gb|KDP42236.1| hypothetical protein
JCGZ_02966 [Jatropha curcas]
Length = 407
Score = 412 bits (1060), Expect = e-140
Identities = 187/254 (73%), Positives = 211/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW+VAI LA+YG Y KY GEA + EQLA+VTS FTWLTLVPPAHFN
Sbjct: 154 WMYFSWTWLFWPWLVAISLAVYGFYCFYKYSLGEARIFEQLALVTSIFTWLTLVPPAHFN 213
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+LEGWP NVSVRKRLYGDYY R HD+KWD+S P W++L FCVGVMV
Sbjct: 214 GYLEGWPFVFFFVYHYFFFFNVSVRKRLYGDYYARPHDSKWDVSPPKWSRLFFCVGVMVA 273
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHLVPGGW+N GIW+LI+LT+ MQY+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 274 HWLAAFEGPELHLVPGGWSNAGIWILILLTLLMQYNSTLYLAKYSEKVVVPTAVVQFGPY 333
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIY+STMLLF TYF ALRAP+S LF +AVCL+YY QKAK+EEALM+E F E Y E
Sbjct: 334 RWVRHPIYSSTMLLFATYFIALRAPLSLLFVIAVCLIYYAQKAKMEEALMIENFRESYLE 393
Query: 721 YMSKVRYKLIPFVY 762
YMSKV+YK IP VY
Sbjct: 394 YMSKVQYKFIPLVY 407
>ref|XP_011035283.1| PREDICTED: uncharacterized protein LOC105133138 [Populus
euphratica]
Length = 404
Score = 411 bits (1056), Expect = e-139
Identities = 188/254 (74%), Positives = 209/254 (82%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW VAI LA+YG+Y K+ GEA + EQLAIVTS FTWLTLVPPAHFN
Sbjct: 151 WMYFSWTWLFWPWFVAILLAVYGLYCFYKHSIGEARIFEQLAIVTSMFTWLTLVPPAHFN 210
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+L+GWP NVSVRKRLYGDYY R HD KWD+ P W++LLFCVGVMVG
Sbjct: 211 GYLQGWPFVFFFVYHYFFFFNVSVRKRLYGDYYARPHDPKWDLKPPRWSRLLFCVGVMVG 270
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGWNNVGIW+LI+ T MQY+ST YL+KYSEKVVVP AVVQFGPY
Sbjct: 271 HWLAAFEGPELHLIPGGWNNVGIWILILATWLMQYNSTFYLAKYSEKVVVPIAVVQFGPY 330
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIY+STMLLF TYF ALRAP+S LF VAVCLMYY QKAK+EE LM+ETFGE+Y E
Sbjct: 331 RWVRHPIYSSTMLLFATYFLALRAPLSLLFVVAVCLMYYAQKAKMEEDLMIETFGEKYLE 390
Query: 721 YMSKVRYKLIPFVY 762
YM KVRYK IP VY
Sbjct: 391 YMGKVRYKFIPLVY 404
>ref|XP_010262021.1| PREDICTED: uncharacterized protein LOC104600650 [Nelumbo nucifera]
gi|720019202|ref|XP_010262022.1| PREDICTED:
uncharacterized protein LOC104600650 [Nelumbo nucifera]
gi|720019205|ref|XP_010262023.1| PREDICTED:
uncharacterized protein LOC104600650 [Nelumbo nucifera]
gi|720019208|ref|XP_010262024.1| PREDICTED:
uncharacterized protein LOC104600650 [Nelumbo nucifera]
gi|720019211|ref|XP_010262025.1| PREDICTED:
uncharacterized protein LOC104600650 [Nelumbo nucifera]
Length = 414
Score = 410 bits (1055), Expect = e-139
Identities = 189/254 (74%), Positives = 210/254 (82%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW +AI LA+YG+Y L K+ GEAN+ EQL IVTS FTWLTLVPPAHFN
Sbjct: 161 WMYFSWTWLFWPWFLAISLALYGLYCLRKHFHGEANIFEQLVIVTSTFTWLTLVPPAHFN 220
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+LEGWP NVSVRKRLYGDYYPR HD KWD+S PN ++L FCVGVM
Sbjct: 221 GYLEGWPFVFFFVYHYFFFFNVSVRKRLYGDYYPRSHDPKWDVSPPNSHRLGFCVGVMAS 280
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAA EG LHL PGGW+N+GIW+LIM T+FMQYHSTLYL+KYSEKVVVPT+VVQFGPY
Sbjct: 281 HWLAASEGLALHLTPGGWSNLGIWILIMSTLFMQYHSTLYLAKYSEKVVVPTSVVQFGPY 340
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYAST+LLF TY ALRAP+S LF V VCL+YYG KAK EE LM+ETFGERY E
Sbjct: 341 RWVRHPIYASTVLLFSTYCVALRAPLSFLFMVVVCLLYYGYKAKSEEELMVETFGERYME 400
Query: 721 YMSKVRYKLIPFVY 762
YMSKVRYKLIPFVY
Sbjct: 401 YMSKVRYKLIPFVY 414
>ref|XP_008356928.1| PREDICTED: uncharacterized protein LOC103420651 [Malus domestica]
Length = 404
Score = 409 bits (1051), Expect = e-138
Identities = 184/254 (72%), Positives = 213/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTW+FWPW +A+ +A+YG+Y K+L+ EAN+ EQLAIVTS F+WLTLVPPAHFN
Sbjct: 151 WMYFSWTWMFWPWFLAVSVAVYGLYCFRKHLRCEANVFEQLAIVTSVFSWLTLVPPAHFN 210
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVSVRKRLYGDYY R HD KWD+S P W +LLFCVGVM G
Sbjct: 211 GFLEGWPYVFFFVYHYFFFFNVSVRKRLYGDYYARPHDPKWDVSPPKWLRLLFCVGVMAG 270
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELH +PG W+NV IW+LI++T+ MQY+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 271 HWLAAFEGPELHRIPGKWSNVPIWILILVTLLMQYNSTLYLAKYSEKVVVPTAVVQFGPY 330
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLF TYF ALRAP+S LF VAVC +YY QKAK+EEALM+ETFGERY++
Sbjct: 331 RWVRHPIYASTMLLFATYFIALRAPLSLLFIVAVCSLYYDQKAKMEEALMVETFGERYTD 390
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYK IPF+Y
Sbjct: 391 YASKVRYKFIPFIY 404
>ref|XP_004294649.1| PREDICTED: uncharacterized protein LOC101293565 [Fragaria vesca
subsp. vesca]
Length = 396
Score = 409 bits (1050), Expect = e-138
Identities = 186/254 (73%), Positives = 211/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTW+FWPW +A+ + +YG+Y K+L GEA++ EQLA+VTS FTWLTLVPPAHFN
Sbjct: 143 WMYFSWTWMFWPWFLAVSVGLYGLYCFRKHLVGEASMFEQLAVVTSVFTWLTLVPPAHFN 202
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVSVRKRLYGDYY R HD KWD+S P W +LLF VGVM+G
Sbjct: 203 GFLEGWPYVFFLVYHYFFFFNVSVRKRLYGDYYARPHDPKWDVSPPRWYRLLFSVGVMIG 262
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELH +PGGW+NVGIW LI++T+ MQY+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 263 HWLAAFEGPELHRIPGGWSNVGIWGLIVVTLLMQYNSTLYLAKYSEKVVVPTAVVQFGPY 322
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLF TY ALRAP+S LF VAVC+MYY QKAKLEEALM+ETFGERY E
Sbjct: 323 RWVRHPIYASTMLLFATYCVALRAPLSLLFVVAVCVMYYDQKAKLEEALMIETFGERYME 382
Query: 721 YMSKVRYKLIPFVY 762
Y SKV YK IPFVY
Sbjct: 383 YASKVWYKFIPFVY 396
>ref|XP_002314167.1| hypothetical protein POPTR_0009s03890g [Populus trichocarpa]
gi|222850575|gb|EEE88122.1| hypothetical protein
POPTR_0009s03890g [Populus trichocarpa]
Length = 405
Score = 409 bits (1050), Expect = e-138
Identities = 185/254 (72%), Positives = 212/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW VAI LA+YG+Y K+ GEA++ EQLAIVTS FTWLTLVPPAHF+
Sbjct: 152 WMYFSWTWLFWPWFVAISLAVYGLYCFYKHSIGEASIFEQLAIVTSVFTWLTLVPPAHFS 211
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+L+GWP NVSVRKRLYGDYY R HD KWD++ P W +LLFCVGVM G
Sbjct: 212 GYLQGWPFVFFLVYHYFFFFNVSVRKRLYGDYYARPHDPKWDLNPPRWCRLLFCVGVMAG 271
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGW NVGIW+LI+ T+ MQY+ST YL+KYSEKVVVP+AVVQFGPY
Sbjct: 272 HWLAAFEGPELHLIPGGWINVGIWILILATLLMQYNSTFYLAKYSEKVVVPSAVVQFGPY 331
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIY+ST+LLFVTYF ALRAP+S LF VAVCLMYY QKAK+EE LM+ETFGE+Y E
Sbjct: 332 RWVRHPIYSSTLLLFVTYFIALRAPLSLLFVVAVCLMYYAQKAKMEEGLMIETFGEKYLE 391
Query: 721 YMSKVRYKLIPFVY 762
YMSKV+YK IP VY
Sbjct: 392 YMSKVQYKFIPLVY 405
>gb|KNA17899.1| hypothetical protein SOVF_075600 [Spinacia oleracea]
Length = 392
Score = 408 bits (1048), Expect = e-138
Identities = 184/254 (72%), Positives = 214/254 (84%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW VAIGL +YG+Y K+ + EA++VEQLAIVTSAFTWLTLVPPA+FN
Sbjct: 139 WMYFSWTWLFWPWFVAIGLGVYGIYCFRKHSRCEASIVEQLAIVTSAFTWLTLVPPAYFN 198
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVS+RKRLYGDY R HD KWD+S+PNW ++LF VGVMVG
Sbjct: 199 GFLEGWPYVFFFVYHYFFFFNVSIRKRLYGDYNMRPHDPKWDVSVPNWYQVLFGVGVMVG 258
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELHL+PGGW+N+GIW LIM T+FM Y+++LYL+KYSEK+VVPT+VVQFGPY
Sbjct: 259 HWLAAFEGPELHLIPGGWSNIGIWGLIMATLFMHYNASLYLAKYSEKIVVPTSVVQFGPY 318
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLF+TYF ALRAP+S L+T+ VC+MYY QKAKLEE LM +TF E Y E
Sbjct: 319 RWVRHPIYASTMLLFLTYFVALRAPLSCLYTIGVCIMYYDQKAKLEEELMADTFRESYLE 378
Query: 721 YMSKVRYKLIPFVY 762
YMSKVRYK IPFVY
Sbjct: 379 YMSKVRYKFIPFVY 392
>ref|XP_010043508.1| PREDICTED: uncharacterized protein LOC104432703 [Eucalyptus
grandis] gi|629121035|gb|KCW85525.1| hypothetical
protein EUGRSUZ_B02322 [Eucalyptus grandis]
Length = 400
Score = 408 bits (1048), Expect = e-138
Identities = 185/254 (72%), Positives = 211/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSW+WLFWPW+VAI LAIYG+Y L K+L+GEAN+ EQLA+VTSAF WLTLVPPA FN
Sbjct: 147 WMYFSWSWLFWPWIVAIALAIYGLYCLRKHLRGEANIFEQLAVVTSAFAWLTLVPPAQFN 206
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+LEGWP NVSVR+RLYGDYY R HD KWDI P W +LLFCVGVMVG
Sbjct: 207 GYLEGWPFVFFFVYHYFFFFNVSVRRRLYGDYYARPHDPKWDIDPPKWQRLLFCVGVMVG 266
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAA EG ELH +PGGW+NVG+WVLI++T+ M Y+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 267 HWLAALEGPELHRIPGGWSNVGVWVLILITILMHYNSTLYLAKYSEKVVVPTAVVQFGPY 326
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYAST+LLF Y ALRAP+S LF VAVCL+YY QKAKLEEA+M+ETFGERY E
Sbjct: 327 RWVRHPIYASTVLLFAAYCIALRAPLSLLFVVAVCLLYYQQKAKLEEAVMVETFGERYLE 386
Query: 721 YMSKVRYKLIPFVY 762
Y KV+YK IP VY
Sbjct: 387 YAGKVKYKFIPLVY 400
>ref|XP_002299861.2| hypothetical protein POPTR_0001s24920g [Populus trichocarpa]
gi|550348115|gb|EEE84666.2| hypothetical protein
POPTR_0001s24920g [Populus trichocarpa]
Length = 404
Score = 407 bits (1046), Expect = e-137
Identities = 185/254 (72%), Positives = 209/254 (82%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTWLFWPW VAI LA+YG+Y K+ GEA++ EQ AIVTS FTWLTLVPPAHFN
Sbjct: 151 WMYFSWTWLFWPWFVAILLAVYGLYCFYKHSIGEASIFEQFAIVTSVFTWLTLVPPAHFN 210
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+L+GWP NVSVRKRLYGDYY R HD KWD+ P W++LLFCVGVMVG
Sbjct: 211 GYLQGWPFVFFFVYHYFFFFNVSVRKRLYGDYYARPHDPKWDLHPPRWSRLLFCVGVMVG 270
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAA EG ELHL+PGGW+NVGIW+LI+ T+ MQY+ST YL+KYSEKVVVP AVVQFGPY
Sbjct: 271 HWLAALEGPELHLIPGGWSNVGIWILILATLLMQYNSTFYLAKYSEKVVVPIAVVQFGPY 330
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIY+STMLLF TYF ALRAP+S LF VAVCLMYY QKAK+EE LM+ETFGE+Y E
Sbjct: 331 RWVRHPIYSSTMLLFATYFLALRAPLSLLFVVAVCLMYYAQKAKMEEDLMIETFGEKYLE 390
Query: 721 YMSKVRYKLIPFVY 762
YM KVRYK IP VY
Sbjct: 391 YMGKVRYKFIPLVY 404
>ref|XP_007016369.1| C-terminal S-isoprenylcysteine carboxyl O-methyltransferases
[Theobroma cacao] gi|508786732|gb|EOY33988.1| C-terminal
S-isoprenylcysteine carboxyl O-methyltransferases
[Theobroma cacao]
Length = 396
Score = 406 bits (1043), Expect = e-137
Identities = 183/254 (72%), Positives = 213/254 (83%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSW+W+FWPW VA+ LA YG+Y K+ G+A+++EQLAIVTS FTWLTLVPPA+FN
Sbjct: 143 WMYFSWSWMFWPWFVAVALAFYGLYCFRKHSIGKASIIEQLAIVTSVFTWLTLVPPAYFN 202
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
G+LEGWP NVSVRKRLYGDYY R HD KWD++ P W +LLFCVGVMVG
Sbjct: 203 GYLEGWPFVFFFVYHYFFFFNVSVRKRLYGDYYARPHDPKWDVNPPKWYRLLFCVGVMVG 262
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELH +PGGW+NV +W+L++ T+ MQY+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 263 HWLAAFEGPELHRIPGGWSNVVVWILVVATLLMQYNSTLYLAKYSEKVVVPTAVVQFGPY 322
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLF TY ALRAP+S+LF +AVCLMYY QKAKLEEALM+ETFGE YSE
Sbjct: 323 RWVRHPIYASTMLLFATYCVALRAPLSSLFVLAVCLMYYEQKAKLEEALMVETFGESYSE 382
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYK IPFV+
Sbjct: 383 YASKVRYKFIPFVF 396
>ref|XP_009364365.1| PREDICTED: uncharacterized protein LOC103954280 [Pyrus x
bretschneideri]
Length = 404
Score = 406 bits (1043), Expect = e-137
Identities = 181/254 (71%), Positives = 214/254 (84%)
Frame = +1
Query: 1 WMYFSWTWLFWPWMVAIGLAIYGVYSLSKYLKGEANLVEQLAIVTSAFTWLTLVPPAHFN 180
WMYFSWTW+FWPW +A+ +A+YG+Y K+L+GEAN+ EQLAIVTS F+WLTLVPPAHFN
Sbjct: 151 WMYFSWTWMFWPWFLAVLVAVYGLYCFRKHLRGEANVFEQLAIVTSVFSWLTLVPPAHFN 210
Query: 181 GFLEGWPXXXXXXXXXXXXLNVSVRKRLYGDYYPREHDTKWDISLPNWNKLLFCVGVMVG 360
GFLEGWP NVSVRKRLYGDYY R HD KW++S P W ++LFC+GVM G
Sbjct: 211 GFLEGWPYVFFFVYHYFFFFNVSVRKRLYGDYYARPHDPKWNVSPPKWLRILFCLGVMAG 270
Query: 361 HWLAAFEGTELHLVPGGWNNVGIWVLIMLTVFMQYHSTLYLSKYSEKVVVPTAVVQFGPY 540
HWLAAFEG ELH +PG W+NV IW+LI++T+ MQY+STLYL+KYSEKVVVPTAVVQFGPY
Sbjct: 271 HWLAAFEGPELHRIPGKWSNVPIWILILVTLLMQYNSTLYLAKYSEKVVVPTAVVQFGPY 330
Query: 541 RFLRHPIYASTMLLFVTYFTALRAPMSALFTVAVCLMYYGQKAKLEEALMLETFGERYSE 720
R++RHPIYASTMLLF TYF ALRAP+S LF VAVC +YY QKAK+EEALM+ETFG+RY++
Sbjct: 331 RWVRHPIYASTMLLFATYFIALRAPLSLLFIVAVCSLYYDQKAKMEEALMVETFGDRYTD 390
Query: 721 YMSKVRYKLIPFVY 762
Y SKVRYK IPF+Y
Sbjct: 391 YASKVRYKFIPFIY 404