BLASTX nr result
ID: Rehmannia27_contig00017086
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia27_contig00017086
(441 letters)
Database: ./nr
84,704,028 sequences; 31,038,470,784 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011071773.1| PREDICTED: serine/threonine-protein kinase A... 206 8e-62
ref|XP_012831159.1| PREDICTED: serine/threonine-protein kinase A... 185 6e-54
ref|XP_010028436.1| PREDICTED: serine/threonine-protein kinase A... 168 2e-47
gb|KJB18765.1| hypothetical protein B456_003G071400 [Gossypium r... 162 2e-47
gb|KJB18769.1| hypothetical protein B456_003G071400 [Gossypium r... 162 8e-47
gb|KJB18763.1| hypothetical protein B456_003G071400 [Gossypium r... 162 9e-47
gb|KJB18768.1| hypothetical protein B456_003G071400 [Gossypium r... 162 1e-46
gb|KJB18767.1| hypothetical protein B456_003G071400 [Gossypium r... 162 2e-46
gb|KMT10464.1| hypothetical protein BVRB_5g115900 isoform A [Bet... 159 4e-46
gb|KDO69833.1| hypothetical protein CISIN_1g013249mg [Citrus sin... 159 4e-46
ref|NP_001307141.1| serine/threonine-protein kinase AtPK1/AtPK6 ... 164 7e-46
gb|KDO69834.1| hypothetical protein CISIN_1g013249mg [Citrus sin... 159 9e-46
gb|KVI06358.1| AGC-kinase, C-terminal [Cynara cardunculus var. s... 163 1e-45
ref|XP_015079145.1| PREDICTED: serine/threonine-protein kinase A... 163 1e-45
ref|XP_012470310.1| PREDICTED: serine/threonine-protein kinase A... 162 1e-45
ref|XP_009802853.1| PREDICTED: serine/threonine-protein kinase A... 162 2e-45
ref|XP_009617135.1| PREDICTED: serine/threonine-protein kinase A... 162 2e-45
gb|AHW50673.1| serine/threonine-protein kinase [Nicotiana tabacum] 161 5e-45
gb|KDO69831.1| hypothetical protein CISIN_1g013249mg [Citrus sin... 159 6e-45
ref|XP_006354810.1| PREDICTED: serine/threonine-protein kinase A... 160 1e-44
>ref|XP_011071773.1| PREDICTED: serine/threonine-protein kinase AtPK1/AtPK6-like
[Sesamum indicum]
Length = 489
Score = 206 bits (523), Expect = 8e-62
Identities = 112/154 (72%), Positives = 119/154 (77%), Gaps = 8/154 (5%)
Frame = -3
Query: 439 KSLPPFERNEQEELYYSSSDD---NGAKDGVIEKDNGSVCXXXXXXXXXXXXXXXXXG-- 275
KSLP FE++E+EELYYSSSDD N KD E+ +GSVC
Sbjct: 94 KSLPTFEQDEREELYYSSSDDTANNDMKDEAREEGSGSVCGGDGMNGVVENGGIDGVKKV 153
Query: 274 -PGDFEIMRVIGKGAFGKVFQVRMKGKSGD--DGDGIFAMKVMRKDTIIKNNHVDYMRAE 104
P DFEIMRVIGKGAFGKVFQVRMKG+SG DGDGIFAMKVMRKDTIIKNNHVDYMRAE
Sbjct: 154 GPADFEIMRVIGKGAFGKVFQVRMKGRSGGGGDGDGIFAMKVMRKDTIIKNNHVDYMRAE 213
Query: 103 RDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
RDILTKVVHPF+VQLRYSFQTKSKLYLILDFING
Sbjct: 214 RDILTKVVHPFVVQLRYSFQTKSKLYLILDFING 247
>ref|XP_012831159.1| PREDICTED: serine/threonine-protein kinase AtPK1/AtPK6-like
[Erythranthe guttata] gi|604343650|gb|EYU42504.1|
hypothetical protein MIMGU_mgv1a005346mg [Erythranthe
guttata]
Length = 488
Score = 185 bits (470), Expect = 6e-54
Identities = 105/154 (68%), Positives = 111/154 (72%), Gaps = 8/154 (5%)
Frame = -3
Query: 439 KSLPPFERNEQEELYYSSSDDNGAKDGVIEK-DNGSVCXXXXXXXXXXXXXXXXXGPGDF 263
+SLPPFE +ELYYSSSDD +D E + G GPGDF
Sbjct: 95 RSLPPFE---PDELYYSSSDDAANRDSNHESGEKGGGGIACGGGGDSIVEEVKKVGPGDF 151
Query: 262 EIMRVIGKGAFGKVFQVRMKGKSGD-------DGDGIFAMKVMRKDTIIKNNHVDYMRAE 104
EIMR+IGKGAFGKVFQVRMK +SG DGDGIFAMKVMRKDTIIKN HVDYMRAE
Sbjct: 152 EIMRMIGKGAFGKVFQVRMKAESGGGGGGGGGDGDGIFAMKVMRKDTIIKNKHVDYMRAE 211
Query: 103 RDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
RDILTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 212 RDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 245
>ref|XP_010028436.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Eucalyptus grandis] gi|629118971|gb|KCW83461.1|
hypothetical protein EUGRSUZ_B00380 [Eucalyptus grandis]
gi|629118972|gb|KCW83462.1| hypothetical protein
EUGRSUZ_B00380 [Eucalyptus grandis]
Length = 492
Score = 168 bits (426), Expect = 2e-47
Identities = 89/145 (61%), Positives = 102/145 (70%)
Frame = -3
Query: 436 SLPPFERNEQEELYYSSSDDNGAKDGVIEKDNGSVCXXXXXXXXXXXXXXXXXGPGDFEI 257
SLP + +E +E D+ AK G++E + GPGDFEI
Sbjct: 105 SLPFIDSSEDDE---GDDGDDDAKGGILEGNERESSDLGGVGGREIAEGGAKIGPGDFEI 161
Query: 256 MRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDILTKVVH 77
+RV+GKGAFGKVFQVR KG G +GDGI+AMKVMRK+TIIK NHVDYM+AERDIL KVVH
Sbjct: 162 LRVVGKGAFGKVFQVRKKGGHGGEGDGIYAMKVMRKETIIKKNHVDYMKAERDILAKVVH 221
Query: 76 PFIVQLRYSFQTKSKLYLILDFING 2
PFIV LRYSFQTKSKLYLILDFING
Sbjct: 222 PFIVPLRYSFQTKSKLYLILDFING 246
>gb|KJB18765.1| hypothetical protein B456_003G071400 [Gossypium raimondii]
gi|763751378|gb|KJB18766.1| hypothetical protein
B456_003G071400 [Gossypium raimondii]
Length = 276
Score = 162 bits (411), Expect = 2e-47
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>gb|KJB18769.1| hypothetical protein B456_003G071400 [Gossypium raimondii]
Length = 324
Score = 162 bits (411), Expect = 8e-47
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>gb|KJB18763.1| hypothetical protein B456_003G071400 [Gossypium raimondii]
Length = 326
Score = 162 bits (411), Expect = 9e-47
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>gb|KJB18768.1| hypothetical protein B456_003G071400 [Gossypium raimondii]
gi|763751382|gb|KJB18770.1| hypothetical protein
B456_003G071400 [Gossypium raimondii]
Length = 330
Score = 162 bits (411), Expect = 1e-46
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>gb|KJB18767.1| hypothetical protein B456_003G071400 [Gossypium raimondii]
Length = 359
Score = 162 bits (411), Expect = 2e-46
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>gb|KMT10464.1| hypothetical protein BVRB_5g115900 isoform A [Beta vulgaris subsp.
vulgaris]
Length = 274
Score = 159 bits (403), Expect = 4e-46
Identities = 83/97 (85%), Positives = 86/97 (88%), Gaps = 6/97 (6%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKS---GDD---GDGIFAMKVMRKDTIIKNNHVDYM 113
P DFEI+RVIGKGAFGKVFQVR KG+ GDD GDGIFAMKVMRKD IIK NHVDYM
Sbjct: 138 PEDFEILRVIGKGAFGKVFQVRKKGEDCEIGDDYDEGDGIFAMKVMRKDNIIKKNHVDYM 197
Query: 112 RAERDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
+AERDILTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 198 KAERDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 234
>gb|KDO69833.1| hypothetical protein CISIN_1g013249mg [Citrus sinensis]
Length = 254
Score = 159 bits (401), Expect = 4e-46
Identities = 76/91 (83%), Positives = 82/91 (90%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
PGDFEI RV+G+GAFGKVFQV+ KG D DGI+AMKVMRKDTIIK NHVDYM+AERDI
Sbjct: 109 PGDFEIFRVVGQGAFGKVFQVKKKGVDDGDSDGIYAMKVMRKDTIIKKNHVDYMKAERDI 168
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKV HPFIVQLRYSFQT+SKLYLILDFING
Sbjct: 169 LTKVTHPFIVQLRYSFQTRSKLYLILDFING 199
>ref|NP_001307141.1| serine/threonine-protein kinase AtPK1/AtPK6 [Solanum lycopersicum]
gi|62183960|gb|AAX73301.1| putative ribosomal-protein S6
kinase-like protein [Solanum lycopersicum]
Length = 466
Score = 164 bits (414), Expect = 7e-46
Identities = 80/92 (86%), Positives = 87/92 (94%), Gaps = 1/92 (1%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGD-DGDGIFAMKVMRKDTIIKNNHVDYMRAERD 98
PGDFEI+R+IGKG+FGKVFQV+MKG G+ +GDGI AMKVMRKDT+IKNNHVDYMRAERD
Sbjct: 133 PGDFEILRMIGKGSFGKVFQVKMKGYGGEGEGDGILAMKVMRKDTVIKNNHVDYMRAERD 192
Query: 97 ILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
ILTKV HPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 193 ILTKVEHPFIVQLRYSFQTKSKLYLILDFING 224
>gb|KDO69834.1| hypothetical protein CISIN_1g013249mg [Citrus sinensis]
Length = 282
Score = 159 bits (401), Expect = 9e-46
Identities = 76/91 (83%), Positives = 82/91 (90%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
PGDFEI RV+G+GAFGKVFQV+ KG D DGI+AMKVMRKDTIIK NHVDYM+AERDI
Sbjct: 109 PGDFEIFRVVGQGAFGKVFQVKKKGVDDGDSDGIYAMKVMRKDTIIKKNHVDYMKAERDI 168
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKV HPFIVQLRYSFQT+SKLYLILDFING
Sbjct: 169 LTKVTHPFIVQLRYSFQTRSKLYLILDFING 199
>gb|KVI06358.1| AGC-kinase, C-terminal [Cynara cardunculus var. scolymus]
Length = 462
Score = 163 bits (412), Expect = 1e-45
Identities = 84/98 (85%), Positives = 87/98 (88%), Gaps = 7/98 (7%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKS-------GDDGDGIFAMKVMRKDTIIKNNHVDY 116
PGDFEIMRVIGKG+FGKVFQVR K +S G GDGIFAMKVMRKDTIIKNNHVDY
Sbjct: 135 PGDFEIMRVIGKGSFGKVFQVRRKDRSCVNGEVEGVIGDGIFAMKVMRKDTIIKNNHVDY 194
Query: 115 MRAERDILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
MRAERDILTKVVHPFIVQLRYSFQTK+KLYLILDFING
Sbjct: 195 MRAERDILTKVVHPFIVQLRYSFQTKTKLYLILDFING 232
>ref|XP_015079145.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Solanum pennellii]
Length = 467
Score = 163 bits (412), Expect = 1e-45
Identities = 80/91 (87%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
PGDFEI+R+IGKG+FGKVFQV+MKG G+ GDGI AMKVMRKDT+IKNNHVDYMRAERDI
Sbjct: 136 PGDFEILRMIGKGSFGKVFQVKMKGYGGE-GDGILAMKVMRKDTVIKNNHVDYMRAERDI 194
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKV HPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 195 LTKVEHPFIVQLRYSFQTKSKLYLILDFING 225
>ref|XP_012470310.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Gossypium raimondii] gi|763751376|gb|KJB18764.1|
hypothetical protein B456_003G071400 [Gossypium
raimondii]
Length = 450
Score = 162 bits (411), Expect = 1e-45
Identities = 78/91 (85%), Positives = 86/91 (94%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
P DFEI+RV+G+GAFGKVFQVR KG++ +DGDG+FAMKVM+K TIIK NHVDYMRAERDI
Sbjct: 118 PEDFEILRVVGQGAFGKVFQVRNKGRNNEDGDGLFAMKVMKKVTIIKKNHVDYMRAERDI 177
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKVVHPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 208
>ref|XP_009802853.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Nicotiana sylvestris]
Length = 452
Score = 162 bits (410), Expect = 2e-45
Identities = 80/93 (86%), Positives = 86/93 (92%), Gaps = 2/93 (2%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKG--KSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAER 101
PGDFE++R+IGKG+FGKVFQV+MKG GD DGI+AMKVMRKDTIIKNNHVDYMRAER
Sbjct: 118 PGDFEMLRMIGKGSFGKVFQVKMKGYGDDGDSDDGIYAMKVMRKDTIIKNNHVDYMRAER 177
Query: 100 DILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
DILTKV HPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 178 DILTKVEHPFIVQLRYSFQTKSKLYLILDFING 210
>ref|XP_009617135.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Nicotiana tomentosiformis]
Length = 453
Score = 162 bits (410), Expect = 2e-45
Identities = 80/93 (86%), Positives = 86/93 (92%), Gaps = 2/93 (2%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKG--KSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAER 101
PGDFE++R+IGKG+FGKVFQV+MKG GD DGI+AMKVMRKDTIIKNNHVDYMRAER
Sbjct: 119 PGDFEMLRMIGKGSFGKVFQVKMKGYGDDGDSDDGIYAMKVMRKDTIIKNNHVDYMRAER 178
Query: 100 DILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
DILTKV HPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 179 DILTKVEHPFIVQLRYSFQTKSKLYLILDFING 211
>gb|AHW50673.1| serine/threonine-protein kinase [Nicotiana tabacum]
Length = 451
Score = 161 bits (407), Expect = 5e-45
Identities = 81/93 (87%), Positives = 88/93 (94%), Gaps = 2/93 (2%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDG--DGIFAMKVMRKDTIIKNNHVDYMRAER 101
PGDFE++R+IGKG+FGKVFQV+MKG GDDG DGI+AMKVMRKDTIIKNNHVDYMRAER
Sbjct: 118 PGDFEMLRMIGKGSFGKVFQVKMKGY-GDDGEDDGIYAMKVMRKDTIIKNNHVDYMRAER 176
Query: 100 DILTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
DILTKV HPFIVQLRYSFQT+SKLYLILDFING
Sbjct: 177 DILTKVEHPFIVQLRYSFQTRSKLYLILDFING 209
>gb|KDO69831.1| hypothetical protein CISIN_1g013249mg [Citrus sinensis]
gi|641850960|gb|KDO69832.1| hypothetical protein
CISIN_1g013249mg [Citrus sinensis]
Length = 355
Score = 159 bits (401), Expect = 6e-45
Identities = 76/91 (83%), Positives = 82/91 (90%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
PGDFEI RV+G+GAFGKVFQV+ KG D DGI+AMKVMRKDTIIK NHVDYM+AERDI
Sbjct: 109 PGDFEIFRVVGQGAFGKVFQVKKKGVDDGDSDGIYAMKVMRKDTIIKKNHVDYMKAERDI 168
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKV HPFIVQLRYSFQT+SKLYLILDFING
Sbjct: 169 LTKVTHPFIVQLRYSFQTRSKLYLILDFING 199
>ref|XP_006354810.1| PREDICTED: serine/threonine-protein kinase AtPK2/AtPK19-like
[Solanum tuberosum]
Length = 466
Score = 160 bits (406), Expect = 1e-44
Identities = 79/91 (86%), Positives = 85/91 (93%)
Frame = -3
Query: 274 PGDFEIMRVIGKGAFGKVFQVRMKGKSGDDGDGIFAMKVMRKDTIIKNNHVDYMRAERDI 95
PGDFEI+R+IGKG+FGKVFQV+MKG G+ GDGI AMKVMRKDT+IKN HVDYMRAERDI
Sbjct: 135 PGDFEILRMIGKGSFGKVFQVKMKGYGGE-GDGILAMKVMRKDTVIKNKHVDYMRAERDI 193
Query: 94 LTKVVHPFIVQLRYSFQTKSKLYLILDFING 2
LTKV HPFIVQLRYSFQTKSKLYLILDFING
Sbjct: 194 LTKVEHPFIVQLRYSFQTKSKLYLILDFING 224