BLASTX nr result
ID: Rehmannia26_contig00034602
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia26_contig00034602
(416 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006353561.1| PREDICTED: uncharacterized protein LOC102598... 120 1e-25
ref|XP_006340021.1| PREDICTED: uncharacterized protein LOC102598... 117 2e-24
emb|CAN78967.1| hypothetical protein VITISV_027273 [Vitis vinifera] 109 4e-22
emb|CAN75974.1| hypothetical protein VITISV_028848 [Vitis vinifera] 105 5e-21
emb|CAN62981.1| hypothetical protein VITISV_037893 [Vitis vinifera] 104 1e-20
emb|CAN73394.1| hypothetical protein VITISV_009718 [Vitis vinifera] 103 2e-20
emb|CAN69701.1| hypothetical protein VITISV_020317 [Vitis vinifera] 101 1e-19
emb|CAN66733.1| hypothetical protein VITISV_032667 [Vitis vinifera] 100 2e-19
emb|CAN83929.1| hypothetical protein VITISV_025158 [Vitis vinifera] 99 4e-19
emb|CAN82098.1| hypothetical protein VITISV_022259 [Vitis vinifera] 99 6e-19
ref|XP_004514965.1| PREDICTED: uncharacterized protein LOC101505... 98 1e-18
ref|XP_004514665.1| PREDICTED: uncharacterized protein LOC101494... 98 1e-18
ref|XP_004514311.1| PREDICTED: uncharacterized protein LOC101496... 98 1e-18
ref|XP_004514230.1| PREDICTED: uncharacterized protein LOC101490... 98 1e-18
ref|XP_004514040.1| PREDICTED: uncharacterized protein LOC101509... 98 1e-18
ref|XP_004513813.1| PREDICTED: uncharacterized protein LOC101489... 98 1e-18
ref|XP_004513298.1| PREDICTED: uncharacterized protein LOC101505... 98 1e-18
ref|XP_004506983.1| PREDICTED: uncharacterized protein LOC101507... 98 1e-18
ref|XP_004506973.1| PREDICTED: uncharacterized protein LOC101503... 98 1e-18
ref|XP_004506385.1| PREDICTED: uncharacterized protein LOC101515... 98 1e-18
>ref|XP_006353561.1| PREDICTED: uncharacterized protein LOC102598401 [Solanum tuberosum]
Length = 1105
Score = 120 bits (302), Expect = 1e-25
Identities = 55/118 (46%), Positives = 75/118 (63%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L KLK+YV NK PEGSIAEGYI +ECL FCSMY+ DIET+F++ RN +
Sbjct: 460 YRWMYKIERFLCKLKRYVRNKARPEGSIAEGYIIDECLTFCSMYLTDIETRFNREHRNDD 519
Query: 175 SCRDISKQSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
+ L +FS+N+R G+Y + +K Y+ NC+E PF+ EH+EEL
Sbjct: 520 GSSSKDEHVLDIFSKNSRPFRDGIYDVIPKKDFDMARWYVFNNCEEAEPFLQEHREEL 577
>ref|XP_006340021.1| PREDICTED: uncharacterized protein LOC102598429 [Solanum tuberosum]
Length = 445
Score = 117 bits (292), Expect = 2e-24
Identities = 56/118 (47%), Positives = 76/118 (64%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L KLK+YV NK PEGSIAEGYI +ECL FCSMY+ +IET+F++ +RN +
Sbjct: 30 YRWMYKIERFLCKLKRYVRNKARPEGSIAEGYIIDECLTFCSMYLTNIETRFNREDRNVD 89
Query: 175 SCRDISKQSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
+ + L +FSE+ R KG Y + +K Y+L NC+E PF+ EHK EL
Sbjct: 90 GSSNKEEHVLDIFSESVRPF-KGEYDAIPKKDFDMAQWYVLNNCEEAEPFLQEHKNEL 146
>emb|CAN78967.1| hypothetical protein VITISV_027273 [Vitis vinifera]
Length = 1163
Score = 109 bits (272), Expect = 4e-22
Identities = 55/119 (46%), Positives = 70/119 (58%), Gaps = 1/119 (0%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y RYL LK YV NK P GSI EGYITEEC FCS Y+ D+ET+ D+ ERNY
Sbjct: 392 YRWMYPIERYLRTLKSYVCNKSXPXGSIVEGYITEECTTFCSRYLRDVETKHDREERNYV 451
Query: 175 SCRDISK-QSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
+I+ L++F R IGK L + Q H Y+L NC+EV F+ EHK+ +
Sbjct: 452 IENNITNGGGLTIFKCMGRTIGKSTSRVLSTEEWSQAHLYVLTNCEEVTSFIEEHKQSI 510
>emb|CAN75974.1| hypothetical protein VITISV_028848 [Vitis vinifera]
Length = 463
Score = 105 bits (263), Expect = 5e-21
Identities = 51/119 (42%), Positives = 70/119 (58%), Gaps = 1/119 (0%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y RYL+ LK YV NK PEGSI EGYI +EC FC Y++D+ET+ D+ E+NY
Sbjct: 20 YRWMYPIERYLHTLKSYVRNKSCPEGSIVEGYIAKECTTFCPRYLHDVETKHDREEKNYV 79
Query: 175 SCRDISK-QSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
+I+ L++F IGK L + Q H Y+L NC+EV F+ EHK+ +
Sbjct: 80 IENNITNGGGLTIFKCMGHTIGKSTSHVLSTEEWSQAHLYVLTNCEEVTSFIEEHKQSI 138
>emb|CAN62981.1| hypothetical protein VITISV_037893 [Vitis vinifera]
Length = 618
Score = 104 bits (259), Expect = 1e-20
Identities = 50/109 (45%), Positives = 66/109 (60%)
Frame = -2
Query: 328 YLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYESCRDISKQS 149
YL LK YV NK PEGSI EGYI E+C+ FCS Y++D+ET+ D ERNY +I+
Sbjct: 65 YLCTLKSYVRNKSHPEGSIVEGYIVEKCITFCSRYLHDVETKHDCEERNYVIENNITNGG 124
Query: 148 LSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
L++F IGK L + Q H Y+L NC+EV F+ EHK+ +
Sbjct: 125 LTIFKFMGCTIGKSTSHVLSTEEWFQAHLYVLTNCEEVTSFIEEHKQSI 173
>emb|CAN73394.1| hypothetical protein VITISV_009718 [Vitis vinifera]
Length = 861
Score = 103 bits (257), Expect = 2e-20
Identities = 52/110 (47%), Positives = 66/110 (60%), Gaps = 1/110 (0%)
Frame = -2
Query: 328 YLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYESCRDISK-Q 152
YL LK YV NK PEGSI EGYI EEC FCS Y++D+ET+ D+ ERNY +I+
Sbjct: 318 YLRTLKSYVRNKSRPEGSIXEGYIAEECTTFCSRYLHDVETKHDREERNYVIENNITNGG 377
Query: 151 SLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
L++F R IGK L + Q H Y+L NC EV F+ EHK+ +
Sbjct: 378 GLTIFKCMGRTIGKSTSRVLSTEEWSQAHLYVLTNCXEVXSFIEEHKQSI 427
>emb|CAN69701.1| hypothetical protein VITISV_020317 [Vitis vinifera]
Length = 1208
Score = 101 bits (251), Expect = 1e-19
Identities = 50/119 (42%), Positives = 70/119 (58%), Gaps = 1/119 (0%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y +YL+ LK YV NK PEG IA+GYI +EC FC Y++D+ET+ D+ ERNY
Sbjct: 466 YRWMYPIEQYLHTLKSYVRNKSRPEGFIADGYIAKECTTFCLRYLHDVETKHDREERNYV 525
Query: 175 SCRDISK-QSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEEL 2
+I+ L++F IGK L + Q H Y+L NC+EV F+ EHK+ +
Sbjct: 526 IENNITNGGGLTIFKCMRCTIGKSTSRVLSTEEWSQAHLYVLTNCEEVTSFIEEHKQSI 584
>emb|CAN66733.1| hypothetical protein VITISV_032667 [Vitis vinifera]
Length = 852
Score = 100 bits (250), Expect = 2e-19
Identities = 52/114 (45%), Positives = 71/114 (62%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y F R L LK+YV NK PEGSIAE YI E LMFCSMY+ I T+F++ ERN +
Sbjct: 628 YRWMYPFERNLRTLKRYVRNKARPEGSIAEAYIVNEALMFCSMYLTRIGTRFNRSERNED 687
Query: 175 SCRDISKQSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
D + LS+FS+ AR +G + ++ L + H YI+ NC E+ P++ EH
Sbjct: 688 RFEDQVQGCLSIFSQQARPLGSRQHLQFSKEELTKAHWYIMNNCPELRPYL-EH 740
>emb|CAN83929.1| hypothetical protein VITISV_025158 [Vitis vinifera]
Length = 2249
Score = 99.4 bits (246), Expect = 4e-19
Identities = 50/110 (45%), Positives = 69/110 (62%), Gaps = 1/110 (0%)
Frame = -2
Query: 331 RYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYESCRDISK- 155
+YL+ LK YV+NK PE SIAEGYI EEC +FCS Y++D+ET+ D+ ERNY +I+
Sbjct: 1648 KYLHTLKSYVHNKSRPEDSIAEGYIAEECTIFCSRYLHDVETKHDREERNYVIENNITNG 1707
Query: 154 QSLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHKEE 5
L++F IGK L + Q H Y+L NC+EV+ F+ +K E
Sbjct: 1708 GGLTIFKCMGHTIGKSTSRVLSIEEWSQAHLYVLTNCEEVMSFIELYKCE 1757
>emb|CAN82098.1| hypothetical protein VITISV_022259 [Vitis vinifera]
Length = 1025
Score = 99.0 bits (245), Expect = 6e-19
Identities = 51/107 (47%), Positives = 65/107 (60%), Gaps = 1/107 (0%)
Frame = -2
Query: 328 YLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYESCRDISK-Q 152
YL LK YV NK PEGSIAEGYI EEC FCS Y++D+ET+ D ERNY +I+
Sbjct: 402 YLRTLKSYVRNKSRPEGSIAEGYIAEECTTFCSRYLHDVETKHDCEERNYVIENNITNGG 461
Query: 151 SLSVFSENARLIGKGVYSFLDEKSLKQIHAYILKNCDEVLPFMSEHK 11
L++F R IGK L + Q H Y+L NC+EV+ F+ +K
Sbjct: 462 GLTIFKCMGRTIGKSTSRVLSTEKWSQAHLYVLTNCEEVMSFIELYK 508
>ref|XP_004514965.1| PREDICTED: uncharacterized protein LOC101505760 [Cicer arietinum]
Length = 1321
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 985 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 1044
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 1045 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 1098
>ref|XP_004514665.1| PREDICTED: uncharacterized protein LOC101494663 [Cicer arietinum]
Length = 900
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 564 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 623
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 624 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 677
>ref|XP_004514311.1| PREDICTED: uncharacterized protein LOC101496078 [Cicer arietinum]
Length = 453
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 86 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 145
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 146 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 199
>ref|XP_004514230.1| PREDICTED: uncharacterized protein LOC101490031 [Cicer arietinum]
Length = 980
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 644 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 703
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 704 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 757
>ref|XP_004514040.1| PREDICTED: uncharacterized protein LOC101509911 [Cicer arietinum]
Length = 1095
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 774 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 833
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 834 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 887
>ref|XP_004513813.1| PREDICTED: uncharacterized protein LOC101489594 [Cicer arietinum]
Length = 975
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 736 YRWMYPIERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 795
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 796 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 849
>ref|XP_004513298.1| PREDICTED: uncharacterized protein LOC101505308 [Cicer arietinum]
Length = 957
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 718 YRWMYPIERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 777
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 778 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 831
>ref|XP_004506983.1| PREDICTED: uncharacterized protein LOC101507116 [Cicer arietinum]
gi|502167453|ref|XP_004514145.1| PREDICTED:
uncharacterized protein LOC101504349 [Cicer arietinum]
Length = 975
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 736 YRWMYPIERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 795
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 796 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 849
>ref|XP_004506973.1| PREDICTED: uncharacterized protein LOC101503554 [Cicer arietinum]
Length = 1356
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 1020 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 1079
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 1080 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 1133
>ref|XP_004506385.1| PREDICTED: uncharacterized protein LOC101515037 [Cicer arietinum]
Length = 706
Score = 98.2 bits (243), Expect = 1e-18
Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 3/117 (2%)
Frame = -2
Query: 355 YSLLYVFYRYLYKLKQYVNNKVFPEGSIAEGYITEECLMFCSMYMNDIETQFDKVERNYE 176
Y +Y R+L LK +V N+ PEGSIAEG++ ECL FCS Y++ +ET+F++ RN +
Sbjct: 370 YRWMYPLERFLLTLKSFVRNRAHPEGSIAEGFLANECLTFCSQYLSGVETRFNRPNRNDD 429
Query: 175 SCRDISKQSLSVFSENARLIGKG---VYSFLDEKSLKQIHAYILKNCDEVLPFMSEH 14
++S + L + + +GK + LD+K L Q H Y+L NCD V PF+ EH
Sbjct: 430 ---EVSGRPLGIKKQQKLRLGKRKKVSRTKLDKKELAQAHRYVLSNCDAVAPFIEEH 483