BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia25_contig00016664
         (723 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|...   325   e-101
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like ...   320   2e-99
gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]         317   2e-98
gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]               318   3e-98
gb|AAY84833.1| isoamylase [Ipomoea batatas]                           310   2e-96
ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like ...   307   2e-94
ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like ...   307   2e-94
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...   307   2e-94
ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like ...   305   2e-94
ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, par...   307   2e-94
ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|2...   306   2e-94
dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Pha...   306   7e-94
ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like ...   300   3e-93
ref|XP_003630623.1| Isoamylase [Medicago truncatula] gi|35552464...   303   3e-93
gb|EXC12839.1| Isoamylase 1 [Morus notabilis]                         300   4e-93
ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like ...   298   2e-92
gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [...   301   2e-92
ref|XP_002308090.2| isoamylase family protein [Populus trichocar...   299   8e-92
ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Popu...   299   8e-92
emb|CBI40669.3| unnamed protein product [Vitis vinifera]              294   2e-91

>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|27728145|gb|AAN15317.1|
           isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  325 bits (834), Expect(2) = e-101
 Identities = 145/179 (81%), Positives = 165/179 (92%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G YFDSS++++DPYAKA+VSRGEYG LGP+ DCWP MA +VPS +++FDWEGDLPLK 
Sbjct: 163 EEGHYFDSSQIVLDPYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKF 222

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DLVIYEMHVRGFT HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 223 PQRDLVIYEMHVRGFTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEY 282

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNSVLGDYK NFWGYST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEV
Sbjct: 283 YSYNSVLGDYKFNFWGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEV 341



 Score = 68.9 bits (167), Expect(2) = e-101
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP+L FRG+DNSVFY LAP+
Sbjct: 340 EVIMDVVFNHTAEGNENGPILSFRGIDNSVFYTLAPK 376


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like [Solanum lycopersicum]
          Length = 787

 Score =  320 bits (821), Expect(2) = 2e-99
 Identities = 143/179 (79%), Positives = 164/179 (91%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G YFDSS++++DPYAKA+VSRGEYG LGP+ DCWP MA +VPS +++FDWEGDLPLK 
Sbjct: 157 EEGHYFDSSQIVLDPYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKF 216

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
            Q +LVIYEMHVRGFT HESS +KYPGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 217 SQRNLVIYEMHVRGFTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEY 276

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNSVLGDYK NFWGYST+NFFSPM RYSS+G+ N GLGA+NEFKYLV+EAHKRGIEV
Sbjct: 277 YSYNSVLGDYKFNFWGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEV 335



 Score = 68.9 bits (167), Expect(2) = 2e-99
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP+L FRG+DNSVFY LAP+
Sbjct: 334 EVIMDVVFNHTAEGNENGPILSFRGIDNSVFYTLAPK 370


>gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]
          Length = 774

 Score =  317 bits (812), Expect(2) = 2e-98
 Identities = 143/179 (79%), Positives = 165/179 (92%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           EDG YFDSSK+LIDPYAKA+  RGEYGAL PD +CWPQMA +VPST +EFDWEGDLPL+L
Sbjct: 147 EDGLYFDSSKILIDPYAKAIAGRGEYGALAPDGNCWPQMASMVPSTVDEFDWEGDLPLRL 206

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ +L+IYEMHVRGFTRHESS +++PGTY G+VEKL++LKK+GVNCIELMPC EFNELE+
Sbjct: 207 PQRELIIYEMHVRGFTRHESSMTEFPGTYSGVVEKLEYLKKIGVNCIELMPCQEFNELEY 266

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYN VLGDYKMNFWGYS INFFSPMARYSS+G+ N+GLGAVNEFK+LV+EAHK+GIEV
Sbjct: 267 YSYNRVLGDYKMNFWGYSPINFFSPMARYSSAGVQNHGLGAVNEFKHLVKEAHKKGIEV 325



 Score = 69.3 bits (168), Expect(2) = 2e-98
 Identities = 32/36 (88%), Positives = 34/36 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAP 720
           +VIMDVVFNHTAEGNENGP L FRG+DNSVFYMLAP
Sbjct: 324 EVIMDVVFNHTAEGNENGPTLSFRGVDNSVFYMLAP 359


>gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score =  318 bits (814), Expect(2) = 3e-98
 Identities = 141/179 (78%), Positives = 165/179 (92%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E G Y+DSSK+L+DPYAKAV+SRGE+GALGP+ +CWPQMAC+VP++ +EFDWEGDLPL+ 
Sbjct: 165 EKGHYYDSSKILLDPYAKAVISRGEFGALGPEDNCWPQMACMVPTSEDEFDWEGDLPLRY 224

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
            Q DL+IYEMHVRGFTRHESS +++PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 225 SQRDLIIYEMHVRGFTRHESSRTEFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEY 284

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNS+LGDYK+NFWGYST+NFFSPM RYSSSG+ N G  AVNEFKYLV+EAHKRGIEV
Sbjct: 285 YSYNSILGDYKLNFWGYSTVNFFSPMIRYSSSGMHNCGRDAVNEFKYLVKEAHKRGIEV 343



 Score = 68.2 bits (165), Expect(2) = 3e-98
 Identities = 30/37 (81%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V MDVVFNHTAEGNENGP+L FRG+DNSV+YMLAP+
Sbjct: 342 EVFMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPK 378


>gb|AAY84833.1| isoamylase [Ipomoea batatas]
          Length = 785

 Score =  310 bits (794), Expect(2) = 2e-96
 Identities = 139/179 (77%), Positives = 163/179 (91%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G YFDS+++L+DPYAKA+VSR E+GALGP+KDCWP MAC++P T ++FDWEGDLPLK 
Sbjct: 166 EEGHYFDSARILLDPYAKAIVSRAEFGALGPEKDCWPPMACMLP-TADKFDWEGDLPLKF 224

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DLVIYEMHVRGFT HESS +++PGTY G+VEKLDHLK LGVNCIELMPCHEFNELE+
Sbjct: 225 PQRDLVIYEMHVRGFTNHESSGTEFPGTYRGVVEKLDHLKVLGVNCIELMPCHEFNELEY 284

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYN VLGDYK+NFWGYST+NFFSPM RYSS+G+   GLGA++EFKYLVREAH+RGIEV
Sbjct: 285 YSYNPVLGDYKVNFWGYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEV 343



 Score = 69.3 bits (168), Expect(2) = 2e-96
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP+  FRG+DNSVFYMLAP+
Sbjct: 342 EVIMDVVFNHTAEGNENGPMFSFRGVDNSVFYMLAPK 378


>ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  307 bits (786), Expect(2) = 2e-94
 Identities = 138/182 (75%), Positives = 166/182 (91%), Gaps = 3/182 (1%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNE---EFDWEGDLP 173
           ++G YFDSS++L+DPYAKAV+SRGE+GALGP+ +CWPQMA +VP+ NE   +FDWEGDLP
Sbjct: 174 QEGCYFDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLP 233

Query: 174 LKLPQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNE 353
           ++  Q DL+IYEMHVRGFTRHESS S++PGTYLG+VEKLD+LK+LG+NCIELMPCHEFNE
Sbjct: 234 VQHAQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNE 293

Query: 354 LEHYSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGI 533
           LE++SYNSVLGDYKMNFWGYST+N+FSPM RYSS+GIGN G  A+NEFK L+REAHKRGI
Sbjct: 294 LEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGI 353

Query: 534 EV 539
           EV
Sbjct: 354 EV 355



 Score = 66.2 bits (160), Expect(2) = 2e-94
 Identities = 29/36 (80%), Positives = 34/36 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAP 720
           +VIMDVVFNHTAEGNENGP + FRG+DN+V+YMLAP
Sbjct: 354 EVIMDVVFNHTAEGNENGPTISFRGVDNNVYYMLAP 389


>ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  307 bits (786), Expect(2) = 2e-94
 Identities = 138/182 (75%), Positives = 166/182 (91%), Gaps = 3/182 (1%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNE---EFDWEGDLP 173
           ++G YFDSS++L+DPYAKAV+SRGE+GALGP+ +CWPQMA +VP+ NE   +FDWEGDLP
Sbjct: 174 QEGCYFDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLP 233

Query: 174 LKLPQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNE 353
           ++  Q DL+IYEMHVRGFTRHESS S++PGTYLG+VEKLD+LK+LG+NCIELMPCHEFNE
Sbjct: 234 VQHAQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNE 293

Query: 354 LEHYSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGI 533
           LE++SYNSVLGDYKMNFWGYST+N+FSPM RYSS+GIGN G  A+NEFK L+REAHKRGI
Sbjct: 294 LEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGI 353

Query: 534 EV 539
           EV
Sbjct: 354 EV 355



 Score = 66.2 bits (160), Expect(2) = 2e-94
 Identities = 29/36 (80%), Positives = 34/36 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAP 720
           +VIMDVVFNHTAEGNENGP + FRG+DN+V+YMLAP
Sbjct: 354 EVIMDVVFNHTAEGNENGPTISFRGVDNNVYYMLAP 389


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score =  307 bits (787), Expect(2) = 2e-94
 Identities = 135/179 (75%), Positives = 162/179 (90%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           ++G YFD +K+++DPYAKAV+SR ++G LGPD++CWPQMACLVP+  +EFDWEGDLPLK 
Sbjct: 175 QEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACLVPTPEDEFDWEGDLPLKY 234

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYE+HVRGFTRHESS +++PGTYLG+VEKLDHLK LG+NC+ELMPCHEFNELE+
Sbjct: 235 PQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDLGINCLELMPCHEFNELEY 294

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           +SYNSVLGDYK+NFWGYSTIN+FSPM  YSS+GI N G  A+NEFK LVREAHKRGIEV
Sbjct: 295 FSYNSVLGDYKVNFWGYSTINYFSPMISYSSAGIRNCGHDAINEFKLLVREAHKRGIEV 353



 Score = 65.9 bits (159), Expect(2) = 2e-94
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V+MDVVFNHTAEGN+ GP+L FRG+DNSV+YMLAP+
Sbjct: 352 EVVMDVVFNHTAEGNDKGPILSFRGVDNSVYYMLAPK 388


>ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like [Glycine max]
          Length = 798

 Score =  305 bits (782), Expect(2) = 2e-94
 Identities = 136/178 (76%), Positives = 160/178 (89%)
 Frame = +3

Query: 6   DGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKLP 185
           +G YFDSS +L+DPYAKAV+SRGE+GALGPD +CWPQMA  VPS ++EFDWEGDLPLK P
Sbjct: 169 EGHYFDSSHILLDPYAKAVISRGEFGALGPDGNCWPQMAGTVPSEDDEFDWEGDLPLKYP 228

Query: 186 QCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHY 365
           Q DLVIYEMHVRGFT+HESSN+K+PGTYLG+VEKLDHLK+LGVNC+ELMPCHEFNELE+Y
Sbjct: 229 QKDLVIYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEYY 288

Query: 366 SYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
            +NS  GDY++NFWGYSTIN+FSPM RYSS+GI N G   +NE K+L++EAHKRGIEV
Sbjct: 289 GHNSAQGDYRVNFWGYSTINYFSPMIRYSSAGIRNCGQDGINEIKFLIKEAHKRGIEV 346



 Score = 67.8 bits (164), Expect(2) = 2e-94
 Identities = 29/37 (78%), Positives = 36/37 (97%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP++ FRG+DNS++YMLAP+
Sbjct: 345 EVIMDVVFNHTAEGNENGPIISFRGVDNSMYYMLAPK 381


>ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, partial [Citrus
           clementina] gi|557556429|gb|ESR66443.1| hypothetical
           protein CICLE_v100074892mg, partial [Citrus clementina]
          Length = 624

 Score =  307 bits (787), Expect(2) = 2e-94
 Identities = 135/179 (75%), Positives = 162/179 (90%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           ++G YFD +K+++DPYAKAV+SR ++G LGPD++CWPQMACLVP+  +EFDWEGDLPLK 
Sbjct: 175 QEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACLVPTPEDEFDWEGDLPLKY 234

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYE+HVRGFTRHESS +++PGTYLG+VEKLDHLK LG+NC+ELMPCHEFNELE+
Sbjct: 235 PQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDLGINCLELMPCHEFNELEY 294

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           +SYNSVLGDYK+NFWGYSTIN+FSPM  YSS+GI N G  A+NEFK LVREAHKRGIEV
Sbjct: 295 FSYNSVLGDYKVNFWGYSTINYFSPMISYSSAGIRNCGHDAINEFKLLVREAHKRGIEV 353



 Score = 65.9 bits (159), Expect(2) = 2e-94
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V+MDVVFNHTAEGN+ GP+L FRG+DNSV+YMLAP+
Sbjct: 352 EVVMDVVFNHTAEGNDKGPILSFRGVDNSVYYMLAPK 388


>ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|223530627|gb|EEF32503.1|
           isoamylase, putative [Ricinus communis]
          Length = 795

 Score =  306 bits (785), Expect(2) = 2e-94
 Identities = 140/179 (78%), Positives = 161/179 (89%), Gaps = 1/179 (0%)
 Frame = +3

Query: 6   DGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNE-EFDWEGDLPLKL 182
           DG YFDSS++++DPYAKAV+SRGE+G LGP  +CWPQMA ++PS ++ EFDWEGDLPLK 
Sbjct: 167 DGHYFDSSQIVLDPYAKAVISRGEFGVLGPGDNCWPQMAGMIPSASQAEFDWEGDLPLKY 226

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DLVIYEMHVRGFTRHESS +++PGT+L  VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 227 PQKDLVIYEMHVRGFTRHESSRTEFPGTFLATVEKLDHLKELGVNCIELMPCHEFNELEY 286

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNSVLGDYKMNFWGYSTIN+FSPM RYSS+G  N G  A+NEFK+LVREAHKRGIEV
Sbjct: 287 YSYNSVLGDYKMNFWGYSTINYFSPMTRYSSAGTRNCGRDAINEFKFLVREAHKRGIEV 345



 Score = 66.2 bits (160), Expect(2) = 2e-94
 Identities = 29/37 (78%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGN+ GP+L FRG+DNSV+YMLAP+
Sbjct: 344 EVIMDVVFNHTAEGNQKGPILSFRGVDNSVYYMLAPK 380


>dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Phaseolus vulgaris]
           gi|561033644|gb|ESW32223.1| hypothetical protein
           PHAVU_002G303600g [Phaseolus vulgaris]
          Length = 791

 Score =  306 bits (783), Expect(2) = 7e-94
 Identities = 135/178 (75%), Positives = 163/178 (91%)
 Frame = +3

Query: 6   DGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKLP 185
           +G Y+DSS++++DPYAKAV+SRGE+GALGP+ +CWPQMA +VPS ++EFDWEGDLPLK P
Sbjct: 162 EGHYYDSSRIVLDPYAKAVISRGEFGALGPNGNCWPQMAGMVPSDHDEFDWEGDLPLKYP 221

Query: 186 QCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEHY 365
           Q DLV+YEMHVRGFT+HESSN+K+PGTYLG+VEKLDHLK+LGVNC+ELMPCHEFNELE+ 
Sbjct: 222 QKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEYS 281

Query: 366 SYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           SYNSV GDY++NFWGYSTINFFSPM RYSS+GI N G   +NE K+L++EAHKRGIEV
Sbjct: 282 SYNSVQGDYRVNFWGYSTINFFSPMIRYSSAGIRNCGRDGINEIKFLIKEAHKRGIEV 339



 Score = 65.5 bits (158), Expect(2) = 7e-94
 Identities = 27/37 (72%), Positives = 36/37 (97%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNE+GP++ FRG+DNS++YM+AP+
Sbjct: 338 EVIMDVVFNHTAEGNEDGPIISFRGVDNSIYYMVAPK 374


>ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like [Vitis vinifera]
          Length = 748

 Score =  300 bits (769), Expect(2) = 3e-93
 Identities = 134/179 (74%), Positives = 161/179 (89%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G Y+DSS++L+DPYAKAV+SRGE+G LGP+ +CWP MA ++PS++ EFDWEGDLPLK 
Sbjct: 117 EEGHYYDSSRLLLDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKY 176

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFTRHESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 177 PQKDLIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEY 236

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           +SYNSVL DY++NFWGYST+N+FSPM RYSS+GI N G  A+NE K L+REAHKRGIEV
Sbjct: 237 FSYNSVLDDYRVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEV 295



 Score = 68.9 bits (167), Expect(2) = 3e-93
 Identities = 30/37 (81%), Positives = 36/37 (97%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V+MDVVFNHTAEGNENGP+L FRG+DNSV+YMLAP+
Sbjct: 294 EVLMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPK 330


>ref|XP_003630623.1| Isoamylase [Medicago truncatula] gi|355524645|gb|AET05099.1|
           Isoamylase [Medicago truncatula]
          Length = 822

 Score =  303 bits (777), Expect(2) = 3e-93
 Identities = 134/179 (74%), Positives = 160/179 (89%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           + G Y+DSS++LIDPYAKAV+SRGE+G +GPD +CWPQMA +VP  NEEFDWEGDLPLK 
Sbjct: 159 QQGHYYDSSRILIDPYAKAVISRGEFGEVGPDGNCWPQMAGMVPFDNEEFDWEGDLPLKY 218

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFT+HESS +++PGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 219 PQKDLIIYEMHVRGFTKHESSKAEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEY 278

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYN++ GDY++NFWGYSTIN+FSPM RYSS+GI N G   +NE K L++EAHKRGIEV
Sbjct: 279 YSYNAIQGDYRVNFWGYSTINYFSPMIRYSSAGIQNCGRDGINEMKLLIKEAHKRGIEV 337



 Score = 65.5 bits (158), Expect(2) = 3e-93
 Identities = 28/37 (75%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNE GP++ FRG+DNSV+YM+AP+
Sbjct: 336 EVIMDVVFNHTAEGNEKGPIISFRGVDNSVYYMVAPK 372


>gb|EXC12839.1| Isoamylase 1 [Morus notabilis]
          Length = 805

 Score =  300 bits (767), Expect(2) = 4e-93
 Identities = 132/179 (73%), Positives = 156/179 (87%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G Y+D S++++DPY KAV+ RGEYG LGPD +CWPQMAC VP  + EFDWEGDLPLK 
Sbjct: 161 EEGHYYDPSRIVLDPYGKAVICRGEYGTLGPDGNCWPQMACAVPFFDNEFDWEGDLPLKY 220

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL++YEMHVRGFTRHESS +++PGTYLG+VEKL HL++LGVNCIELMPCHEFNELE+
Sbjct: 221 PQEDLIVYEMHVRGFTRHESSGTEFPGTYLGVVEKLHHLQELGVNCIELMPCHEFNELEY 280

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNS +GDYK+NFWGYST+N+FSPM RYSS+G  N G  AVNE K L+REAHKRGIEV
Sbjct: 281 YSYNSAIGDYKVNFWGYSTVNYFSPMLRYSSAGTHNCGHDAVNELKLLIREAHKRGIEV 339



 Score = 68.9 bits (167), Expect(2) = 4e-93
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP+L FRG DNSV+YMLAP+
Sbjct: 338 EVIMDVVFNHTAEGNENGPILSFRGADNSVYYMLAPK 374


>ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like [Fragaria vesca subsp.
           vesca]
          Length = 794

 Score =  298 bits (762), Expect(2) = 2e-92
 Identities = 130/179 (72%), Positives = 160/179 (89%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G Y+D SK+++DPYAKAV+ RGE+G LGP+ +CWPQMAC+VPS +++F+WEGDLPL  
Sbjct: 164 EEGHYYDPSKIVMDPYAKAVIRRGEFGNLGPNGNCWPQMACMVPSFDDKFNWEGDLPLNY 223

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFTRHESS ++ PGTYLG+VEKLDHLK+LG+NCIELMPCHEFNELE+
Sbjct: 224 PQKDLIIYEMHVRGFTRHESSMTELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEY 283

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           + YNSVLGDYK+N+WGYST+NFFSPM RYSS+G  N G  A+NE K+LV+EAH+RGIEV
Sbjct: 284 FGYNSVLGDYKVNYWGYSTVNFFSPMIRYSSAGTRNCGRDAINEVKFLVKEAHRRGIEV 342



 Score = 68.9 bits (167), Expect(2) = 2e-92
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +VIMDVVFNHTAEGNENGP+L FRG DNSV+YMLAP+
Sbjct: 341 EVIMDVVFNHTAEGNENGPILSFRGADNSVYYMLAPK 377


>gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [Prunus persica]
          Length = 777

 Score =  301 bits (770), Expect(2) = 2e-92
 Identities = 133/179 (74%), Positives = 159/179 (88%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G Y+DSSK+++DPYAKAV+ RGEYG L PD +CWPQMA  VPS ++EFDWEGDLPL  
Sbjct: 122 EEGLYYDSSKIVLDPYAKAVIRRGEYGKLSPDGNCWPQMAGTVPSFDDEFDWEGDLPLNY 181

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYE+HVRGFTRHESS ++ PGTYLG+VEKLDHLK+LG+NCIELMPCHEFNELE+
Sbjct: 182 PQKDLIIYEIHVRGFTRHESSMAELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEY 241

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           + YNSVLGDYK+NFWGYST+N+FSPM RYSS+GI N G  A+NE K+L++EAHKRGIEV
Sbjct: 242 FGYNSVLGDYKVNFWGYSTVNYFSPMIRYSSAGIRNCGRDAINEVKFLIKEAHKRGIEV 300



 Score = 65.5 bits (158), Expect(2) = 2e-92
 Identities = 29/35 (82%), Positives = 33/35 (94%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLA 717
           +VIMDVVFNHTAEGNENGP+L FRG DNS++YMLA
Sbjct: 299 EVIMDVVFNHTAEGNENGPILSFRGADNSIYYMLA 333


>ref|XP_002308090.2| isoamylase family protein [Populus trichocarpa]
           gi|550335674|gb|EEE91613.2| isoamylase family protein
           [Populus trichocarpa]
          Length = 876

 Score =  299 bits (765), Expect(2) = 8e-92
 Identities = 133/179 (74%), Positives = 158/179 (88%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E G Y+D SK+++DPYAK+V+SRGE+G LG D + WPQMAC++P+   +FDWEGD PLK 
Sbjct: 215 EVGLYYDPSKIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKH 274

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFT+HESS +++PGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 275 PQRDLIIYEMHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEY 334

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNSVLGDYK+NFWGYST+N+FSPM RYSS+G  N G  A+NEFK LVREAHKRGIEV
Sbjct: 335 YSYNSVLGDYKVNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEV 393



 Score = 65.5 bits (158), Expect(2) = 8e-92
 Identities = 28/37 (75%), Positives = 34/37 (91%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V MDVVFNHTAEGNE GP+L FRG+DNS++YMLAP+
Sbjct: 392 EVFMDVVFNHTAEGNEKGPILSFRGVDNSIYYMLAPK 428


>ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Populus trichocarpa]
           gi|550335673|gb|ERP58934.1| hypothetical protein
           POPTR_0006s07010g [Populus trichocarpa]
          Length = 845

 Score =  299 bits (765), Expect(2) = 8e-92
 Identities = 133/179 (74%), Positives = 158/179 (88%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E G Y+D SK+++DPYAK+V+SRGE+G LG D + WPQMAC++P+   +FDWEGD PLK 
Sbjct: 215 EVGLYYDPSKIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKH 274

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFT+HESS +++PGTYLG+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 275 PQRDLIIYEMHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEY 334

Query: 363 YSYNSVLGDYKMNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHKRGIEV 539
           YSYNSVLGDYK+NFWGYST+N+FSPM RYSS+G  N G  A+NEFK LVREAHKRGIEV
Sbjct: 335 YSYNSVLGDYKVNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEV 393



 Score = 65.5 bits (158), Expect(2) = 8e-92
 Identities = 28/37 (75%), Positives = 34/37 (91%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V MDVVFNHTAEGNE GP+L FRG+DNS++YMLAP+
Sbjct: 392 EVFMDVVFNHTAEGNEKGPILSFRGVDNSIYYMLAPK 428


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  294 bits (752), Expect(2) = 2e-91
 Identities = 134/185 (72%), Positives = 161/185 (87%), Gaps = 6/185 (3%)
 Frame = +3

Query: 3   EDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNEEFDWEGDLPLKL 182
           E+G Y+DSS++L+DPYAKAV+SRGE+G LGP+ +CWP MA ++PS++ EFDWEGDLPLK 
Sbjct: 172 EEGHYYDSSRLLLDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKY 231

Query: 183 PQCDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDHLKKLGVNCIELMPCHEFNELEH 362
           PQ DL+IYEMHVRGFTRHESS +K+PGTY G+VEKLDHLK+LGVNCIELMPCHEFNELE+
Sbjct: 232 PQKDLIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEY 291

Query: 363 YSYNSVLGDYK------MNFWGYSTINFFSPMARYSSSGIGNYGLGAVNEFKYLVREAHK 524
           +SYNSVL DY+      +NFWGYST+N+FSPM RYSS+GI N G  A+NE K L+REAHK
Sbjct: 292 FSYNSVLDDYRYTCISMVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHK 351

Query: 525 RGIEV 539
           RGIEV
Sbjct: 352 RGIEV 356



 Score = 68.9 bits (167), Expect(2) = 2e-91
 Identities = 30/37 (81%), Positives = 36/37 (97%)
 Frame = +1

Query: 613 KVIMDVVFNHTAEGNENGPLL*FRGLDNSVFYMLAPQ 723
           +V+MDVVFNHTAEGNENGP+L FRG+DNSV+YMLAP+
Sbjct: 355 EVLMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPK 391