BLASTX nr result
ID: Rehmannia25_contig00015743
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia25_contig00015743
(328 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002527931.1| chloroplast phosphate transporter, putative ... 154 1e-35
ref|XP_002314598.2| hypothetical protein POPTR_0010s05630g [Popu... 152 6e-35
gb|ABK91949.1| chloroplast phosphate transporter [Capsicum frute... 151 8e-35
ref|XP_003530627.1| PREDICTED: inorganic phosphate transporter 2... 149 3e-34
gb|ABK91951.1| chloroplast phosphate transporter [Nicotiana taba... 148 6e-34
ref|NP_001275167.1| chloroplast phosphate transporter precursor ... 148 8e-34
ref|XP_003551229.1| PREDICTED: inorganic phosphate transporter 2... 147 1e-33
gb|EXB82536.1| Inorganic phosphate transporter 2-1 [Morus notabi... 147 2e-33
ref|XP_002311765.2| hypothetical protein POPTR_0008s19070g [Popu... 145 4e-33
ref|XP_004509674.1| PREDICTED: inorganic phosphate transporter 2... 145 5e-33
ref|XP_006438300.1| hypothetical protein CICLE_v10031076mg [Citr... 143 3e-32
gb|ABK91950.1| chloroplast phosphate transporter [Solanum melong... 142 4e-32
gb|AAB41897.1| putative phosphate permease [Mesembryanthemum cry... 142 5e-32
ref|XP_002271876.1| PREDICTED: inorganic phosphate transporter 2... 141 8e-32
emb|CAN65936.1| hypothetical protein VITISV_008965 [Vitis vinifera] 141 8e-32
gb|ESW25781.1| hypothetical protein PHAVU_003G064900g [Phaseolus... 140 1e-31
gb|EMJ23191.1| hypothetical protein PRUPE_ppa003252mg [Prunus pe... 138 7e-31
gb|EOY00630.1| Phosphate transporter 2,1 [Theobroma cacao] 137 1e-30
ref|XP_004239128.1| PREDICTED: inorganic phosphate transporter 2... 137 1e-30
ref|XP_003628943.1| Inorganic phosphate transporter 2-1 [Medicag... 136 3e-30
>ref|XP_002527931.1| chloroplast phosphate transporter, putative [Ricinus communis]
gi|223532706|gb|EEF34488.1| chloroplast phosphate
transporter, putative [Ricinus communis]
Length = 476
Score = 154 bits (389), Expect = 1e-35
Identities = 80/109 (73%), Positives = 85/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVF GVTGISFAAFPLSKT P+ AFLV RII KQLGHLL K+ +
Sbjct: 218 APIAVFAGVTGISFAAFPLSKTFPVALVQALACGAAGAFLVYRIIHKQLGHLLVKANTTQ 277
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK T NKNIGFLSDIAGPTGTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 278 PEPKESTIQNKNIGFLSDIAGPTGTQLEIVYGVFGYMQVLSACFMSFAH 326
>ref|XP_002314598.2| hypothetical protein POPTR_0010s05630g [Populus trichocarpa]
gi|550329147|gb|EEF00769.2| hypothetical protein
POPTR_0010s05630g [Populus trichocarpa]
Length = 590
Score = 152 bits (383), Expect = 6e-35
Identities = 79/109 (72%), Positives = 84/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISFAAFPLSK P+ AFLVDRIIRKQLGHLL K++ S
Sbjct: 332 APIAVFLGVTGISFAAFPLSKVFPLALAQALASGAVGAFLVDRIIRKQLGHLLVKASSSQ 391
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK +KNIG LSD AGP GTQLEIVYGVFGYMQILSACFMSFAH
Sbjct: 392 PEPKENAIHSKNIGLLSDFAGPKGTQLEIVYGVFGYMQILSACFMSFAH 440
>gb|ABK91949.1| chloroplast phosphate transporter [Capsicum frutescens]
Length = 148
Score = 151 bits (382), Expect = 8e-35
Identities = 77/109 (70%), Positives = 86/109 (78%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISF A PLSKT + AF+VDRIIR QLGHLLAK++ +
Sbjct: 29 APIAVFLGVTGISFVALPLSKTLNLAVGQAITCGAIGAFVVDRIIRGQLGHLLAKASSKE 88
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+P+PET KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 89 PEPEPETTDTKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 137
>ref|XP_003530627.1| PREDICTED: inorganic phosphate transporter 2-1, chloroplastic-like
[Glycine max]
Length = 575
Score = 149 bits (377), Expect = 3e-34
Identities = 76/109 (69%), Positives = 84/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISF AFPLSK+ P AFLVDRIIRKQLGHLL KS+ +
Sbjct: 317 APIAVFLGVTGISFVAFPLSKSFPFALVQALASGTVGAFLVDRIIRKQLGHLLVKSSTPE 376
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK +T + NIGFL D+AGP G QLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 377 PEPKEDTVHHHNIGFLDDVAGPKGAQLEIVYGVFGYMQVLSACFMSFAH 425
>gb|ABK91951.1| chloroplast phosphate transporter [Nicotiana tabacum]
Length = 148
Score = 148 bits (374), Expect = 6e-34
Identities = 76/109 (69%), Positives = 84/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISF A PLSK + AF+VDRIIR QLGHLLAKS+ +
Sbjct: 29 APIAVFLGVTGISFVALPLSKISNLALGQAITCGAIGAFVVDRIIRGQLGHLLAKSSSEE 88
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+P+ ET KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 89 PEPESETFDTKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 137
>ref|NP_001275167.1| chloroplast phosphate transporter precursor [Solanum tuberosum]
gi|47571341|gb|AAT35816.1| chloroplast phosphate
transporter precursor [Solanum tuberosum]
Length = 581
Score = 148 bits (373), Expect = 8e-34
Identities = 75/109 (68%), Positives = 85/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
API+VFLGVTGISF A PLSKT + AF VDRIIR QLGHLLA+++ +
Sbjct: 323 APISVFLGVTGISFIALPLSKTLNLALGQAITCGAISAFAVDRIIRGQLGHLLARASSKE 382
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+P+PET KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 383 PEPEPETTDTKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 431
>ref|XP_003551229.1| PREDICTED: inorganic phosphate transporter 2-1, chloroplastic-like
[Glycine max]
Length = 576
Score = 147 bits (371), Expect = 1e-33
Identities = 75/109 (68%), Positives = 83/109 (76%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISF AFPLSK P+ AFLVDRIIRKQLGHLL KS +
Sbjct: 318 APIAVFLGVTGISFVAFPLSKNFPLALAQALACGTVGAFLVDRIIRKQLGHLLVKSNTPE 377
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK ++ + NIGFL D+AGP G QLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 378 PEPKEDSVHHHNIGFLDDVAGPKGAQLEIVYGVFGYMQVLSACFMSFAH 426
>gb|EXB82536.1| Inorganic phosphate transporter 2-1 [Morus notabilis]
Length = 575
Score = 147 bits (370), Expect = 2e-33
Identities = 79/109 (72%), Positives = 84/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISFAAFPLSKT P+ AFLVDRIIRKQLGHLL KS S
Sbjct: 317 APIAVFLGVTGISFAAFPLSKTFPVVLAQSLACGTAGAFLVDRIIRKQLGHLLKKSTSSS 376
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+ + NK+IGFLSDIAGP GTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 377 QEEHKDD-TNKSIGFLSDIAGPKGTQLEIVYGVFGYMQVLSACFMSFAH 424
>ref|XP_002311765.2| hypothetical protein POPTR_0008s19070g [Populus trichocarpa]
gi|550333437|gb|EEE89132.2| hypothetical protein
POPTR_0008s19070g [Populus trichocarpa]
Length = 590
Score = 145 bits (367), Expect = 4e-33
Identities = 76/109 (69%), Positives = 84/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISFAAFPLS+ P+ AFLVDRIIRK+LGHLL K++ +
Sbjct: 332 APIAVFLGVTGISFAAFPLSEIFPLALAQALACGTTGAFLVDRIIRKKLGHLLVKASSTQ 391
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+ K +KNIGFLSD AGP GTQLEIVYGVFGYMQILSACFMSFAH
Sbjct: 392 PELKENAIHSKNIGFLSDFAGPKGTQLEIVYGVFGYMQILSACFMSFAH 440
>ref|XP_004509674.1| PREDICTED: inorganic phosphate transporter 2-1, chloroplastic-like
[Cicer arietinum]
Length = 587
Score = 145 bits (366), Expect = 5e-33
Identities = 77/109 (70%), Positives = 85/109 (77%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLG TGISF+AFPLSK+ PI AFLVDR IRKQLGHLL KS
Sbjct: 330 APIAVFLGTTGISFSAFPLSKSFPIALSQALACGTVGAFLVDRTIRKQLGHLLIKSNTPQ 389
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK ++ ++NIGFLSDIAGP GTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 390 PEPKDDS-VHQNIGFLSDIAGPKGTQLEIVYGVFGYMQVLSACFMSFAH 437
>ref|XP_006438300.1| hypothetical protein CICLE_v10031076mg [Citrus clementina]
gi|568860846|ref|XP_006483925.1| PREDICTED: inorganic
phosphate transporter 2-1, chloroplastic-like [Citrus
sinensis] gi|557540496|gb|ESR51540.1| hypothetical
protein CICLE_v10031076mg [Citrus clementina]
Length = 575
Score = 143 bits (360), Expect = 3e-32
Identities = 75/109 (68%), Positives = 81/109 (74%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVF+GVTGISFAAFPLSK P+ AFLV RII+KQLGHLL KS
Sbjct: 317 APIAVFVGVTGISFAAFPLSKIFPLALAQALAFGAAGAFLVYRIIQKQLGHLLVKSTSLQ 376
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+PK NK+IG SDIAGP GTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 377 PEPKDTNIHNKSIGIFSDIAGPKGTQLEIVYGVFGYMQVLSACFMSFAH 425
>gb|ABK91950.1| chloroplast phosphate transporter [Solanum melongena]
Length = 150
Score = 142 bits (359), Expect = 4e-32
Identities = 76/111 (68%), Positives = 84/111 (75%), Gaps = 2/111 (1%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAK--SAK 155
API+VFLGVTGISF A PLSKT + AF V RIIR QLGHLLAK S +
Sbjct: 29 APISVFLGVTGISFVALPLSKTLSLALVQAITYGTIGAFAVHRIIRGQLGHLLAKASSKE 88
Query: 154 SDPDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+P+P+PET KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 89 PEPEPEPETTDTKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 139
>gb|AAB41897.1| putative phosphate permease [Mesembryanthemum crystallinum]
Length = 583
Score = 142 bits (358), Expect = 5e-32
Identities = 76/110 (69%), Positives = 85/110 (77%), Gaps = 1/110 (0%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
AP+AVF+GV+GISFAAFPLSK P+ A +VDR+IRKQLGHLLAKS S+
Sbjct: 325 APVAVFVGVSGISFAAFPLSKILPLALVQALACGTVGAVIVDRMIRKQLGHLLAKSTSSE 384
Query: 148 PDPKPETHPN-KNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
D E HP KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 385 -DEHEEDHPQQKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 433
>ref|XP_002271876.1| PREDICTED: inorganic phosphate transporter 2-1, chloroplastic-like
[Vitis vinifera]
Length = 574
Score = 141 bits (356), Expect = 8e-32
Identities = 76/109 (69%), Positives = 81/109 (74%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
AP+AVF+GVTGISFAAFPL + P AFLV RII KQLGHLL KS S
Sbjct: 316 APLAVFVGVTGISFAAFPLGDSLPSALPKALACGVAGAFLVYRIIHKQLGHLLVKSNSSQ 375
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+ K +T NKNIGFLSDIAGP GTQLEIVYGVFGYMQILSACFMSFAH
Sbjct: 376 LEAKEDTIHNKNIGFLSDIAGPKGTQLEIVYGVFGYMQILSACFMSFAH 424
>emb|CAN65936.1| hypothetical protein VITISV_008965 [Vitis vinifera]
Length = 567
Score = 141 bits (356), Expect = 8e-32
Identities = 76/109 (69%), Positives = 81/109 (74%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
AP+AVF+GVTGISFAAFPL + P AFLV RII KQLGHLL KS S
Sbjct: 309 APLAVFVGVTGISFAAFPLGDSLPSALPKALACGVAGAFLVYRIIHKQLGHLLVKSNSSQ 368
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+ K +T NKNIGFLSDIAGP GTQLEIVYGVFGYMQILSACFMSFAH
Sbjct: 369 LEAKEDTIHNKNIGFLSDIAGPKGTQLEIVYGVFGYMQILSACFMSFAH 417
>gb|ESW25781.1| hypothetical protein PHAVU_003G064900g [Phaseolus vulgaris]
Length = 568
Score = 140 bits (354), Expect = 1e-31
Identities = 74/109 (67%), Positives = 82/109 (75%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVF+GVTGISF AFPLSK+ P+ AFLVDRIIRKQLGHLL KS
Sbjct: 311 APIAVFMGVTGISFVAFPLSKSFPLALAQALVSGTVGAFLVDRIIRKQLGHLLVKSNTPK 370
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P+ K +T + NIGFL D+AGP G QLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 371 PESKEDT-VHHNIGFLDDVAGPKGAQLEIVYGVFGYMQVLSACFMSFAH 418
>gb|EMJ23191.1| hypothetical protein PRUPE_ppa003252mg [Prunus persica]
Length = 589
Score = 138 bits (348), Expect = 7e-31
Identities = 75/109 (68%), Positives = 81/109 (74%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVF+GV+GISFAAFPL + + A LV RIIRKQLGHLL KS S+
Sbjct: 331 APIAVFVGVSGISFAAFPLGENLALALARALACGIAGAVLVYRIIRKQLGHLLLKSTSSE 390
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+ K T KNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH
Sbjct: 391 AEQKEGTIHPKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 439
>gb|EOY00630.1| Phosphate transporter 2,1 [Theobroma cacao]
Length = 585
Score = 137 bits (346), Expect = 1e-30
Identities = 74/110 (67%), Positives = 82/110 (74%), Gaps = 1/110 (0%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKS-AKS 152
APIAVF+GVTGISFAAFPLSK+ I A LV +II KQLGHLL KS + S
Sbjct: 327 APIAVFVGVTGISFAAFPLSKSYAIGLAQALGCGAAGALLVHKIINKQLGHLLVKSISSS 386
Query: 151 DPDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+P+ T NKN+G LSDIAGP GTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 387 HSEPQENTTQNKNLGLLSDIAGPKGTQLEIVYGVFGYMQVLSACFMSFAH 436
>ref|XP_004239128.1| PREDICTED: inorganic phosphate transporter 2-1, chloroplastic-like
[Solanum lycopersicum]
Length = 577
Score = 137 bits (345), Expect = 1e-30
Identities = 74/109 (67%), Positives = 81/109 (74%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
API+VFLGVTGISF A PLSKT + AF VDRIIR QLGHLLAK++ +
Sbjct: 323 APISVFLGVTGISFVALPLSKTLNLALGQAITCGAISAFAVDRIIRGQLGHLLAKASSKE 382
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
P ET KNIGFLSDIAGP GTQL+IVYGVFGYMQ+LSACFMSFAH
Sbjct: 383 P----ETTDTKNIGFLSDIAGPKGTQLKIVYGVFGYMQVLSACFMSFAH 427
>ref|XP_003628943.1| Inorganic phosphate transporter 2-1 [Medicago truncatula]
gi|24079961|gb|AAN46087.1| phosphate transporter PHT2-1
[Medicago truncatula] gi|355522965|gb|AET03419.1|
Inorganic phosphate transporter 2-1 [Medicago
truncatula]
Length = 574
Score = 136 bits (343), Expect = 3e-30
Identities = 75/109 (68%), Positives = 79/109 (72%)
Frame = -1
Query: 328 APIAVFLGVTGISFAAFPLSKTPPIXXXXXXXXXXXXAFLVDRIIRKQLGHLLAKSAKSD 149
APIAVFLGVTGISFAAFPLSK PI AFLV+R IRKQLGHLL KS D
Sbjct: 321 APIAVFLGVTGISFAAFPLSKIFPIALSQALACGTVGAFLVNRTIRKQLGHLLDKSNTPD 380
Query: 148 PDPKPETHPNKNIGFLSDIAGPTGTQLEIVYGVFGYMQILSACFMSFAH 2
+P P+ NI F SDI GP GTQLEIVYGVFGYMQ+LSACFMSFAH
Sbjct: 381 LEPVPQ-----NISFFSDITGPKGTQLEIVYGVFGYMQVLSACFMSFAH 424