BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia25_contig00006428
         (927 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrh...   428   e-117
gb|AGP02119.1| 4-coumarate:CoA ligase [Ocimum basilicum]              423   e-116
gb|ADO16242.1| 4-coumarate-CoA ligase [Ocimum tenuiflorum]            419   e-115
gb|ABA40922.1| 4-coumaroyl CoA ligase [Camellia sinensis]             387   e-105
dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]              383   e-104
gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]             379   e-103
gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]                             377   e-102
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        368   2e-99
gb|EPS72883.1| hypothetical protein M569_01872 [Genlisea aurea]       367   3e-99
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  366   6e-99
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   366   6e-99
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  365   2e-98
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   363   4e-98
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   362   1e-97
gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x P...   362   1e-97
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   362   1e-97
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        361   2e-97
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   361   2e-97
gb|AAT02218.1| 4-coumarate-CoA ligase [Agastache rugosa]              357   3e-96
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          357   4e-96

>gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrhiza]
          Length = 569

 Score =  428 bits (1100), Expect = e-117
 Identities = 211/262 (80%), Positives = 236/262 (90%), Gaps = 2/262 (0%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPE-THVFVSKLPTIPISNHLPLHTYCFQNLSEYP 606
           MLSVA+ E QNPELS+ +L  P  ++PE TH+FVSKLPTIPISN +PLHTYCFQN ++YP
Sbjct: 1   MLSVAAAEAQNPELSSHAL--PQTRSPEETHIFVSKLPTIPISNDIPLHTYCFQNYAQYP 58

Query: 605 DRTCLLVGN-TGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGAS 429
           DR CL+VG   GKSY+FAETHLICR+VAAG SN GI+KGDV+M LLQNCAEFVFTFMGAS
Sbjct: 59  DRPCLIVGTGAGKSYTFAETHLICRKVAAGFSNRGIKKGDVIMVLLQNCAEFVFTFMGAS 118

Query: 428 MIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTID 249
           MIGA+TTTANPFCT+ EIFKQF ASKSK+IVTQ+ YVDKLR+TGD+S+VFGEDFSVVTID
Sbjct: 119 MIGAVTTTANPFCTSNEIFKQFKASKSKMIVTQAMYVDKLRDTGDESIVFGEDFSVVTID 178

Query: 248 NPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQ 69
            PP+GCL FS LSEA+E  AP VEIDPNDAVALPFSSGTTGLPKGVILTHKSLITS+AQQ
Sbjct: 179 APPEGCLPFSALSEADEATAPEVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSIAQQ 238

Query: 68  VDGENPNLYLKPDDVVLCVLPL 3
           VDG+NPNLYLKPDDVVLCVLPL
Sbjct: 239 VDGDNPNLYLKPDDVVLCVLPL 260


>gb|AGP02119.1| 4-coumarate:CoA ligase [Ocimum basilicum]
          Length = 567

 Score =  423 bits (1087), Expect = e-116
 Identities = 213/263 (80%), Positives = 236/263 (89%), Gaps = 3/263 (1%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPET---HVFVSKLPTIPISNHLPLHTYCFQNLSE 612
           MLSVA  E QN ELS+ +L  P  QT E    H+FVSKLP+I I NHLPLHTYCFQNLS+
Sbjct: 1   MLSVA--EAQNSELSSHAL--PQTQTSEETADHIFVSKLPSISIPNHLPLHTYCFQNLSQ 56

Query: 611 YPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGA 432
           + DR CL+ GN GKSYSFA+THLICR+VAAGL+ LGI+KGDV+M LLQNCAEFVF+F+GA
Sbjct: 57  FADRQCLISGNDGKSYSFADTHLICRKVAAGLTKLGIKKGDVIMVLLQNCAEFVFSFIGA 116

Query: 431 SMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTI 252
           SMIGA+TTTANPFCT+KEIFKQFNAS+SKLIVTQSQYVDKLR+TGDDSVVFGEDFSVVTI
Sbjct: 117 SMIGAVTTTANPFCTSKEIFKQFNASRSKLIVTQSQYVDKLRDTGDDSVVFGEDFSVVTI 176

Query: 251 DNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQ 72
           D PP+GCL FSVL EA+E +AP VEIDP+DAVALPFSSGTTGLPKGVILTHKSLITS+AQ
Sbjct: 177 DAPPEGCLDFSVLCEADEADAPDVEIDPDDAVALPFSSGTTGLPKGVILTHKSLITSIAQ 236

Query: 71  QVDGENPNLYLKPDDVVLCVLPL 3
           QVDGENPNLYLKPDDVVLCVLPL
Sbjct: 237 QVDGENPNLYLKPDDVVLCVLPL 259


>gb|ADO16242.1| 4-coumarate-CoA ligase [Ocimum tenuiflorum]
          Length = 567

 Score =  419 bits (1077), Expect = e-115
 Identities = 210/263 (79%), Positives = 235/263 (89%), Gaps = 3/263 (1%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPET---HVFVSKLPTIPISNHLPLHTYCFQNLSE 612
           MLSVA  E QN ELS+ +L  P  Q  +    H+FVSKLP+I I+NHLPLHTYCFQNLS+
Sbjct: 1   MLSVA--EAQNSELSSHAL--PQTQASKETADHIFVSKLPSISIANHLPLHTYCFQNLSQ 56

Query: 611 YPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGA 432
           + DR CL+ GN GKSYSFA+THLICR+VAAGL+ LGI KGDV+M LLQNCAEFVF+F+GA
Sbjct: 57  FADRQCLISGNNGKSYSFADTHLICRKVAAGLTKLGISKGDVIMVLLQNCAEFVFSFIGA 116

Query: 431 SMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTI 252
           SMIGA+TTTANPFCT+KEIFKQFNAS+S+LIVTQSQYVDKLR+TGDDSVVFGEDF+VVTI
Sbjct: 117 SMIGAVTTTANPFCTSKEIFKQFNASRSRLIVTQSQYVDKLRDTGDDSVVFGEDFAVVTI 176

Query: 251 DNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQ 72
           D PP+GCL FSVL EA+E +AP VEIDPNDAVALPFSSGTTGLPKGVILTHKSLITS+AQ
Sbjct: 177 DAPPEGCLDFSVLCEADEADAPDVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSIAQ 236

Query: 71  QVDGENPNLYLKPDDVVLCVLPL 3
           QVDGENPNLYLKPDDVVLCVLPL
Sbjct: 237 QVDGENPNLYLKPDDVVLCVLPL 259


>gb|ABA40922.1| 4-coumaroyl CoA ligase [Camellia sinensis]
          Length = 588

 Score =  387 bits (993), Expect = e-105
 Identities = 191/260 (73%), Positives = 221/260 (85%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPD 603
           MLS AS ET  PE S      P + + +THVF SKLP IPISNHLPLHTY FQNLS++P+
Sbjct: 1   MLSGASAETPKPEFSPPPPQNPQSSSSQTHVFRSKLPHIPISNHLPLHTYRFQNLSQFPN 60

Query: 602 RTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMI 423
           R CLL+G+TGKSYSF+ THL+ R+VA+GLS LGI+KGDV+M LLQNCAEFVF FMGASMI
Sbjct: 61  RPCLLIGSTGKSYSFSXTHLVSRKVASGLSLLGIKKGDVIMLLLQNCAEFVFAFMGASMI 120

Query: 422 GAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTIDNP 243
           GA+TTTANPF T+ E+FKQFNASKSKLI+T SQYVDKLR+ GD+   + ++FSV+TID+P
Sbjct: 121 GAVTTTANPFYTSAEVFKQFNASKSKLIITHSQYVDKLRDAGDNFPNWAKEFSVITIDDP 180

Query: 242 PDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVD 63
           P+ CLHFSVLSEANE E P V IDP+D VALPFSSGTTGLPKGVILTHKSLI+SVAQQVD
Sbjct: 181 PENCLHFSVLSEANESEIPTVSIDPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQVD 240

Query: 62  GENPNLYLKPDDVVLCVLPL 3
           GENPNL LK +D+VLCVLPL
Sbjct: 241 GENPNLRLKGEDMVLCVLPL 260


>dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  383 bits (984), Expect = e-104
 Identities = 186/256 (72%), Positives = 221/256 (86%), Gaps = 1/256 (0%)
 Frame = -2

Query: 767 SVETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPDRTCLL 588
           SVETQ PEL  +       Q+ + H+F S+LP IPI N +PLHTYCFQNL+EY DRTCL+
Sbjct: 3   SVETQKPELEVADSGKTQAQSSQIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDRTCLI 62

Query: 587 VGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMIGAITT 408
           VG+TGK+YSFAETHLICR+VA+GL+ LG++KGDV+M L QNC EFVF+FMGASMIGA+TT
Sbjct: 63  VGSTGKTYSFAETHLICRKVASGLAKLGVKKGDVIMTLFQNCPEFVFSFMGASMIGAVTT 122

Query: 407 TANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTIDNPPDGCL 228
           TANPF T  EIFKQ +ASK+K+++TQS YVDKL+++G++    GEDFSVVTID+PP+ CL
Sbjct: 123 TANPFYTKAEIFKQMSASKAKVVITQSVYVDKLKDSGEEYPKIGEDFSVVTIDDPPENCL 182

Query: 227 HFSVLSEANEKEAPA-VEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENP 51
           HFSVLSEA+E++ PA VEI P+DAVALPFSSGTTGLPKGV+LTHKSLITSVAQQVDGENP
Sbjct: 183 HFSVLSEADEEDMPATVEIAPDDAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENP 242

Query: 50  NLYLKPDDVVLCVLPL 3
           NLYLK DDVVLCVLPL
Sbjct: 243 NLYLKEDDVVLCVLPL 258


>gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
          Length = 575

 Score =  379 bits (973), Expect = e-103
 Identities = 188/266 (70%), Positives = 223/266 (83%), Gaps = 6/266 (2%)
 Frame = -2

Query: 782 MLSVAS-VETQNPELSNS---SLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLS 615
           M+++A  VE Q PELS S       P ++T   H+F SKLP IPISNHLPLHTYCF+NLS
Sbjct: 1   MITIADPVEPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLS 60

Query: 614 EYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMG 435
            +PD+ CL+ G++GK+YSF+ETHLI ++ AAGLSNLGIRKGDV+M LLQNCAEFVF+FMG
Sbjct: 61  SFPDKPCLISGSSGKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMG 120

Query: 434 ASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDS--VVFGEDFSV 261
           ASMIGA++TTANPF T+ EIFKQ+  +++KLIVTQSQYVDKL++T ++      GEDF V
Sbjct: 121 ASMIGAVSTTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKV 180

Query: 260 VTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITS 81
           +TID+PP+ CLHF+VLSEANE E P V IDP+D VALPFSSGTTGLPKGV+LTHKSLITS
Sbjct: 181 ITIDDPPENCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITS 240

Query: 80  VAQQVDGENPNLYLKPDDVVLCVLPL 3
           VAQQVDGENPNLYLK DDVVLCVLPL
Sbjct: 241 VAQQVDGENPNLYLKHDDVVLCVLPL 266


>gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]
          Length = 559

 Score =  377 bits (968), Expect = e-102
 Identities = 187/262 (71%), Positives = 219/262 (83%), Gaps = 2/262 (0%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPD 603
           M+SVA+VE   PELS        +     HVF SKLP IPISNHLPLHTYCF+NLS++ D
Sbjct: 1   MMSVATVEPTKPELSPPQSQNAPSSDETDHVFRSKLPDIPISNHLPLHTYCFENLSDFSD 60

Query: 602 RTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMI 423
           R CL+ G+TGK+YSFAETHL+ R+VAAGLSNLGI+KGDV+M LLQNC EF+F+F+GASMI
Sbjct: 61  RPCLISGSTGKTYSFAETHLVSRKVAAGLSNLGIKKGDVIMTLLQNCPEFIFSFVGASMI 120

Query: 422 GAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETG--DDSVVFGEDFSVVTID 249
           GA+TTTANPF T  EIFKQF+AS++KLI+TQSQYV KLR++   ++S   GEDF V+TID
Sbjct: 121 GAVTTTANPFYTPSEIFKQFSASRAKLIITQSQYVTKLRDSDCCENSKNPGEDFVVITID 180

Query: 248 NPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQ 69
           +PP+ CLHF+VL+EANE E P V I P+D VALPFSSGTTGLPKGVILTHKSL+TSVAQQ
Sbjct: 181 DPPENCLHFTVLAEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQ 240

Query: 68  VDGENPNLYLKPDDVVLCVLPL 3
           VDGENPNLYLK DDVVLCVLPL
Sbjct: 241 VDGENPNLYLKQDDVVLCVLPL 262


>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  368 bits (945), Expect = 2e-99
 Identities = 179/260 (68%), Positives = 217/260 (83%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPD 603
           MLSVAS ETQ P++  S    P N   E  +F SKLP IPISNHLPLHTYCF+ ++E+ D
Sbjct: 1   MLSVASPETQ-PQIP-SPTPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITEFSD 58

Query: 602 RTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMI 423
             CL+ G++G+ Y+FAETHLIC++ AAGLS LGI+KGDV+M LLQN  EF F+FMGASMI
Sbjct: 59  APCLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGASMI 118

Query: 422 GAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTIDNP 243
           GAITTTANPFCT+ EIFKQF+ S +KL++TQSQYVDKLR+TG+     GEDF+V+T+D+P
Sbjct: 119 GAITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITVDDP 178

Query: 242 PDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVD 63
           P+ CLHFSV+S A+E+E P+V IDP+D V++PFSSGTTGLPKGV LTHKSLI+SVAQQVD
Sbjct: 179 PESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQQVD 238

Query: 62  GENPNLYLKPDDVVLCVLPL 3
           GENPNLYLK DD+VLCVLPL
Sbjct: 239 GENPNLYLKTDDIVLCVLPL 258


>gb|EPS72883.1| hypothetical protein M569_01872 [Genlisea aurea]
          Length = 360

 Score =  367 bits (943), Expect = 3e-99
 Identities = 187/261 (71%), Positives = 215/261 (82%), Gaps = 1/261 (0%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPD 603
           MLSVAS+E Q+P  + SSL L  +   + HVFVSK P I I+N LPLHTYCFQNL  + D
Sbjct: 1   MLSVASLEAQSPTETASSLALRRDHDDDDHVFVSKFPPISITNDLPLHTYCFQNLHHFSD 60

Query: 602 RTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMI 423
            TCL+VG++GK++S+AE HLI RRVAAGL+  GI KGDVVMALLQNCAEFVFTFMGASMI
Sbjct: 61  NTCLIVGSSGKTFSYAEVHLISRRVAAGLTRFGIGKGDVVMALLQNCAEFVFTFMGASMI 120

Query: 422 GAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTIDNP 243
           GA+ T  NPFCT+KEIFKQF A++ K+IVTQS YVDKLRE  +DS+ FGED+ V  ID  
Sbjct: 121 GAVLTMGNPFCTSKEIFKQFKAAEGKVIVTQSIYVDKLRE--EDSLRFGEDYRVFAIDGS 178

Query: 242 PDGCLHFSVLSEANEKEAPA-VEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQV 66
           P+GC+ FS LS A+E E P+ +EIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQV
Sbjct: 179 PEGCVPFSELSGADENEIPSEIEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQV 238

Query: 65  DGENPNLYLKPDDVVLCVLPL 3
           DGENPN +LK DDVVLCVLPL
Sbjct: 239 DGENPNFHLKSDDVVLCVLPL 259


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  366 bits (940), Expect = 6e-99
 Identities = 179/259 (69%), Positives = 216/259 (83%), Gaps = 4/259 (1%)
 Frame = -2

Query: 767 SVETQNPELSN---SSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPDRT 597
           S+ETQNP++SN   S  +       + HVF SKLP IPISNHLPLHTYCF+N S++ DR 
Sbjct: 3   SIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRP 62

Query: 596 CLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMIGA 417
           CL+VG+T K+YSFAETHLI R+V AG ++LG+++GDVVM LLQNCAEF F+F+GASM+GA
Sbjct: 63  CLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGA 122

Query: 416 ITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVV-FGEDFSVVTIDNPP 240
           +TTTANPF T+ EIFKQ NASK+K++VTQ+QYVDKLR+  D  V   GE F+V+TID+PP
Sbjct: 123 VTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPP 182

Query: 239 DGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDG 60
           + C+HFSV+SEANE E P V I+ +D VALPFSSGTTGLPKGV+LTHKSLITSVAQQVDG
Sbjct: 183 ENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDG 242

Query: 59  ENPNLYLKPDDVVLCVLPL 3
           ENPNL+L PDDVVLCVLPL
Sbjct: 243 ENPNLHLTPDDVVLCVLPL 261


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  366 bits (940), Expect = 6e-99
 Identities = 179/259 (69%), Positives = 216/259 (83%), Gaps = 4/259 (1%)
 Frame = -2

Query: 767 SVETQNPELSN---SSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEYPDRT 597
           S+ETQNP++SN   S  +       + HVF SKLP IPISNHLPLHTYCF+N S++ DR 
Sbjct: 3   SIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRP 62

Query: 596 CLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASMIGA 417
           CL+VG+T K+YSFAETHLI R+V AG ++LG+++GDVVM LLQNCAEF F+F+GASM+GA
Sbjct: 63  CLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGA 122

Query: 416 ITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVV-FGEDFSVVTIDNPP 240
           +TTTANPF T+ EIFKQ NASK+K++VTQ+QYVDKLR+  D  V   GE F+V+TID+PP
Sbjct: 123 VTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPP 182

Query: 239 DGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDG 60
           + C+HFSV+SEANE E P V I+ +D VALPFSSGTTGLPKGV+LTHKSLITSVAQQVDG
Sbjct: 183 ENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDG 242

Query: 59  ENPNLYLKPDDVVLCVLPL 3
           ENPNL+L PDDVVLCVLPL
Sbjct: 243 ENPNLHLTPDDVVLCVLPL 261


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  365 bits (936), Expect = 2e-98
 Identities = 195/296 (65%), Positives = 219/296 (73%), Gaps = 36/296 (12%)
 Frame = -2

Query: 782 MLSVAS---VETQNP-ELSNSSLVLP-------HNQTPET-----------------HVF 687
           M+S+AS   VETQ P E+S+ +L+          NQ P+                  HVF
Sbjct: 1   MISIASNNSVETQKPAEISSPNLISDIHPTSSQQNQVPDNIHSPPTTAATTTTTTTNHVF 60

Query: 686 VSKLPTIPISNHLPLHTYCFQNLSEYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNL 507
            SKLP IPI NHLPLHTYCFQ LSE+ DR CL+V +TGKSYSF++T+LI ++  AGLSNL
Sbjct: 61  KSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQKTGAGLSNL 120

Query: 506 GIRKGDVVMALLQNCAEFVFTFMGASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQS 327
           GI+KGDV+M LLQNCAEFVF FMGASMIGAITTTANPF TA EIFKQF  S +KLI+T S
Sbjct: 121 GIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNAKLIITHS 180

Query: 326 QYVDKLRETGDDSVV--------FGEDFSVVTIDNPPDGCLHFSVLSEANEKEAPAVEID 171
           Q+VDKLRE   D            GE F VVT+D+PP+ CLHFSV+SEANEKE P V ID
Sbjct: 181 QFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANEKELPEVVID 240

Query: 170 PNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKPDDVVLCVLPL 3
           P D VALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPL
Sbjct: 241 PEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPL 296


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
           [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  363 bits (933), Expect = 4e-98
 Identities = 194/278 (69%), Positives = 218/278 (78%), Gaps = 18/278 (6%)
 Frame = -2

Query: 782 MLSVAS--------VETQN--PELSNSSLVLPHNQTPET----HVFVSKLPTIP-ISNHL 648
           MLS+AS        VET +  P L ++S    H  T  T    HVF SKLP +P ISNHL
Sbjct: 1   MLSIASNTNNTSVVVETADISPTLISASPEKQHPATSTTLHDHHVFKSKLPDLPNISNHL 60

Query: 647 PLHTYCFQNLSEYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQ 468
           PLHTYCF+N+S++ +R CL+VG+TGK+YSF+ETHLI ++  AGLS LGI KGDV+M LLQ
Sbjct: 61  PLHTYCFENVSDFSERPCLIVGSTGKTYSFSETHLISQKTGAGLSKLGITKGDVIMILLQ 120

Query: 467 NCAEFVFTFMGASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDS 288
           NCAEFVF FMGASMIGA+TTTANPF TA EIFKQF AS +KLI+TQS YVDKLR +G DS
Sbjct: 121 NCAEFVFAFMGASMIGAVTTTANPFYTASEIFKQFKASNAKLIITQSIYVDKLRHSGPDS 180

Query: 287 VVF---GEDFSVVTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPK 117
             +   GE F VVTID+PP+ CLHFSVLS ANE E P V IDP D VALPFSSGTTGLPK
Sbjct: 181 DQYPKLGEHFQVVTIDDPPENCLHFSVLSGANESELPEVSIDPEDPVALPFSSGTTGLPK 240

Query: 116 GVILTHKSLITSVAQQVDGENPNLYLKPDDVVLCVLPL 3
           GVILTHKSLITSVAQQVDGENPNLYLK DDVVLCVLPL
Sbjct: 241 GVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPL 278


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
           gi|118487236|gb|ABK95446.1| unknown [Populus
           trichocarpa] gi|222862690|gb|EEF00197.1| 4-coumarate-CoA
           ligase family protein [Populus trichocarpa]
          Length = 570

 Score =  362 bits (929), Expect = 1e-97
 Identities = 186/267 (69%), Positives = 217/267 (81%), Gaps = 7/267 (2%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPET-----HVFVSKLPTIPISNHLPLHTYCFQNL 618
           M+SVA+VE   PELS      P NQ   +     H+F SKLP I ISNHLPLH YCF+NL
Sbjct: 1   MMSVATVEPPKPELSP-----PQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENL 55

Query: 617 SEYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFM 438
           S++ DR CL+ G+TGK+YSFAETHLI R+VAAGLSNLGI+KGDV+M LLQNC EFVF+FM
Sbjct: 56  SDFSDRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFM 115

Query: 437 GASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETG--DDSVVFGEDFS 264
           GASMIGA+TTT NPF T  EIFKQF+AS++KLI+TQSQ+V+KLR++   +++    EDF 
Sbjct: 116 GASMIGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFI 175

Query: 263 VVTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLIT 84
           V+TID+PP+ CLHF+VL EANE E P V I P+D VALPFSSGTTGLPKGVILTHKSLIT
Sbjct: 176 VITIDDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLIT 235

Query: 83  SVAQQVDGENPNLYLKPDDVVLCVLPL 3
           SVAQQVDGE PNLYLK DDVVLCVLPL
Sbjct: 236 SVAQQVDGEIPNLYLKQDDVVLCVLPL 262


>gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x Populus deltoides]
          Length = 579

 Score =  362 bits (929), Expect = 1e-97
 Identities = 186/267 (69%), Positives = 217/267 (81%), Gaps = 7/267 (2%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPET-----HVFVSKLPTIPISNHLPLHTYCFQNL 618
           M+SVA+VE   PELS      P NQ   +     H+F SKLP I ISNHLPLH YCF+NL
Sbjct: 1   MMSVATVEPPKPELSP-----PQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENL 55

Query: 617 SEYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFM 438
           S++ DR CL+ G+TGK+YSFAETHLI R+VAAGLSNLGI+KGDV+M LLQNC EFVF+FM
Sbjct: 56  SDFSDRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFM 115

Query: 437 GASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETG--DDSVVFGEDFS 264
           GASMIGA+TTT NPF T  EIFKQF+AS++KLI+TQSQ+V+KLR++   +++    EDF 
Sbjct: 116 GASMIGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDYHENNQKPEEDFI 175

Query: 263 VVTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLIT 84
           V+TID+PP+ CLHF+VL EANE E P V I P+D VALPFSSGTTGLPKGVILTHKSLIT
Sbjct: 176 VITIDDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLIT 235

Query: 83  SVAQQVDGENPNLYLKPDDVVLCVLPL 3
           SVAQQVDGE PNLYLK DDVVLCVLPL
Sbjct: 236 SVAQQVDGEIPNLYLKQDDVVLCVLPL 262


>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
           gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
           putative [Ricinus communis]
          Length = 573

 Score =  362 bits (928), Expect = 1e-97
 Identities = 183/265 (69%), Positives = 216/265 (81%), Gaps = 5/265 (1%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVL---PHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSE 612
           M+S+AS+E    ELS+SS      P + + ETH+F SKLP IPISNHLPLHTYCF+NL +
Sbjct: 1   MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 611 YPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGA 432
             D+ CL+ G+TGK+YSFAETHLI ++ AAGLS+LGI+KGDV+M LLQNC EFVF+FMGA
Sbjct: 61  LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 431 SMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDD-SVVFGEDFSVVT 255
           SMIGA+ TTANPF T+ EIFKQ N+S+ KLI+TQS YVDKLR+         GEDF+V+T
Sbjct: 121 SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 254 IDNPPDGCLHFSVLSEANEKEAP-AVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITSV 78
           ID PP+ CLHF+VLSEA+E E P  +  +P+D VALPFSSGTTGLPKGVILTHKSLITSV
Sbjct: 181 IDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSV 240

Query: 77  AQQVDGENPNLYLKPDDVVLCVLPL 3
           AQQVDGENPNLYLK DDVVLCVLPL
Sbjct: 241 AQQVDGENPNLYLKEDDVVLCVLPL 265


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  361 bits (926), Expect = 2e-97
 Identities = 194/296 (65%), Positives = 221/296 (74%), Gaps = 36/296 (12%)
 Frame = -2

Query: 782 MLSVAS--VETQNPELSNSSLVLPH-NQTPE-------------------------THVF 687
           M+S+AS  VETQ P  ++++L+ P  N T +                          HVF
Sbjct: 1   MISIASNSVETQKPADTSTNLMPPLINSTSQQNLTQLQPPACTNNIIDSTTATATINHVF 60

Query: 686 VSKLPTIPISNHLPLHTYCFQNLSEYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNL 507
            SKLP IPI NHLPLHTYCFQNL E+ D+ CL+VG+TGKSYSF+ETHLI ++  AGLSNL
Sbjct: 61  RSKLPDIPIPNHLPLHTYCFQNLPEFSDKPCLIVGSTGKSYSFSETHLISQKTGAGLSNL 120

Query: 506 GIRKGDVVMALLQNCAEFVFTFMGASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQS 327
           GI+KGDV+M LLQNCAEFVF FMGAS+IGA+TTTANPF T+ EIFKQ  A+ +KLI+TQS
Sbjct: 121 GIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKLIITQS 180

Query: 326 QYVDKLRE-----TGDDSVVF---GEDFSVVTIDNPPDGCLHFSVLSEANEKEAPAVEID 171
           QYVDKLRE      G D   +   GEDF VVTID+PP+ CLHFSV SEANEKE P V ID
Sbjct: 181 QYVDKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELPDVVID 240

Query: 170 PNDAVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKPDDVVLCVLPL 3
             D VALPFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPNLYLK DDVVLCVLPL
Sbjct: 241 AEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCVLPL 296


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  361 bits (926), Expect = 2e-97
 Identities = 185/266 (69%), Positives = 216/266 (81%), Gaps = 7/266 (2%)
 Frame = -2

Query: 779 LSVASVETQNPELSNSSLVLPHNQTPET-----HVFVSKLPTIPISNHLPLHTYCFQNLS 615
           +SVA+VE   PELS      P NQ   +     H+F SKLP I ISNHLPLH YCF+NLS
Sbjct: 1   MSVATVEPPKPELSP-----PQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLS 55

Query: 614 EYPDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMG 435
           ++ DR CL+ G+TGK+YSFAETHLI R+VAAGLSNLGI+KGDV+M LLQNC EFVF+FMG
Sbjct: 56  DFSDRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMG 115

Query: 434 ASMIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETG--DDSVVFGEDFSV 261
           ASMIGA+TTTANPF T  EIFKQF+AS++KLI+TQSQYV+KL ++   +++   GEDF V
Sbjct: 116 ASMIGAVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIV 175

Query: 260 VTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITS 81
           +TID+PP+ C HF+VL EANE E P + I P+D VALPFSSGTTGLPKGVILTHKSLITS
Sbjct: 176 ITIDDPPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITS 235

Query: 80  VAQQVDGENPNLYLKPDDVVLCVLPL 3
           VAQQVDGE PNLYLK DDVVLCVLPL
Sbjct: 236 VAQQVDGEIPNLYLKQDDVVLCVLPL 261


>gb|AAT02218.1| 4-coumarate-CoA ligase [Agastache rugosa]
          Length = 553

 Score =  357 bits (917), Expect = 3e-96
 Identities = 189/266 (71%), Positives = 212/266 (79%), Gaps = 6/266 (2%)
 Frame = -2

Query: 782 MLSVASVETQNPELSNSSLVLPHNQTPET-HVFVSKLPTIPISNHLPLHTYCFQNLSEYP 606
           MLSVAS ETQNPELS+ +L        ET H+FVSKLPTIPISNHLPLHTYCF+N S+YP
Sbjct: 1   MLSVASAETQNPELSSHALQPQTQSCEETNHIFVSKLPTIPISNHLPLHTYCFENFSQYP 60

Query: 605 DRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASM 426
           DR CLLVG+ GKSYSFAETHLICR+VAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASM
Sbjct: 61  DRPCLLVGSDGKSYSFAETHLICRKVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGASM 120

Query: 425 IGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRETGDDSVVFGEDFSVVTIDN 246
           IGA+ TTANPFCT+KEIFKQFNASKSK+IVTQS YVDKLR+TGDDS+     F +   D 
Sbjct: 121 IGAVITTANPFCTSKEIFKQFNASKSKMIVTQSMYVDKLRDTGDDSLRLRRGF-LRRHDR 179

Query: 245 PPDGCLHFSVLSEANE-----KEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLITS 81
            P G +   + +         + + ++   P+   +L    GTTGLPKGVILTHKSLITS
Sbjct: 180 QPAGEMPAFLRANGGRRGRRPRRSRSIPTTPSRCRSL----GTTGLPKGVILTHKSLITS 235

Query: 80  VAQQVDGENPNLYLKPDDVVLCVLPL 3
           +AQQVDGENPNLYLKPDDVVLCVLPL
Sbjct: 236 IAQQVDGENPNLYLKPDDVVLCVLPL 261


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  357 bits (916), Expect = 4e-96
 Identities = 181/267 (67%), Positives = 215/267 (80%), Gaps = 7/267 (2%)
 Frame = -2

Query: 782 MLSVAS--VETQNPELSNSSLVLPHNQTPETHVFVSKLPTIPISNHLPLHTYCFQNLSEY 609
           M+S+A   VE ++P+    S      +TP  H+F SKLP IPISNHLPLHTYCF+NL   
Sbjct: 1   MISIADHPVELESPKPDQLS-----PETPADHIFRSKLPDIPISNHLPLHTYCFENLPSL 55

Query: 608 PDRTCLLVGNTGKSYSFAETHLICRRVAAGLSNLGIRKGDVVMALLQNCAEFVFTFMGAS 429
            D+ CL+ G++GK YSF+ETHLI ++ AAGLSNLGI+KGD +M LL NCAEFVF FMGAS
Sbjct: 56  SDKPCLISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGAS 115

Query: 428 MIGAITTTANPFCTAKEIFKQFNASKSKLIVTQSQYVDKLRET----GDDSV-VFGEDFS 264
           MIGA++TTANPF T+KEIFKQF A+++KLI+TQSQYVDKL++T    GD++    GEDF 
Sbjct: 116 MIGAVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFK 175

Query: 263 VVTIDNPPDGCLHFSVLSEANEKEAPAVEIDPNDAVALPFSSGTTGLPKGVILTHKSLIT 84
           V+TID+PP+ CL FS+LSEANE E P V I P+D VALPFSSGTTGLPKGV+LTHKSLIT
Sbjct: 176 VITIDDPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLIT 235

Query: 83  SVAQQVDGENPNLYLKPDDVVLCVLPL 3
           SVAQQVDGENPNLYL  DDVVLCVLPL
Sbjct: 236 SVAQQVDGENPNLYLTHDDVVLCVLPL 262