BLASTX nr result
ID: Rehmannia23_contig00010480
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia23_contig00010480
(329 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004240770.1| PREDICTED: auxin-induced protein 5NG4-like [... 133 2e-29
ref|XP_006343754.1| PREDICTED: WAT1-related protein At2g39510-li... 132 6e-29
gb|EOY18240.1| Nodulin MtN21 /EamA-like transporter family prote... 128 7e-28
gb|EPS61790.1| hypothetical protein M569_13004 [Genlisea aurea] 128 9e-28
gb|EOY18239.1| Nodulin MtN21 /EamA-like transporter family prote... 125 6e-27
ref|XP_006436050.1| hypothetical protein CICLE_v10031857mg [Citr... 124 1e-26
ref|XP_002315243.2| hypothetical protein POPTR_0010s21630g [Popu... 123 3e-26
ref|XP_004302750.1| PREDICTED: auxin-induced protein 5NG4-like [... 121 8e-26
ref|XP_006436052.1| hypothetical protein CICLE_v10031808mg [Citr... 121 1e-25
ref|XP_002274909.2| PREDICTED: auxin-induced protein 5NG4-like [... 120 2e-25
emb|CBI32681.3| unnamed protein product [Vitis vinifera] 120 2e-25
gb|EXB67895.1| Auxin-induced protein 5NG4 [Morus notabilis] 119 5e-25
ref|XP_006410931.1| hypothetical protein EUTSA_v10017814mg [Eutr... 119 5e-25
ref|XP_004307548.1| PREDICTED: auxin-induced protein 5NG4-like [... 119 5e-25
ref|XP_006429015.1| hypothetical protein CICLE_v10012001mg [Citr... 117 1e-24
ref|XP_004236759.1| PREDICTED: auxin-induced protein 5NG4-like [... 117 1e-24
ref|XP_006365719.1| PREDICTED: WAT1-related protein At2g39510-li... 117 2e-24
ref|XP_006294412.1| hypothetical protein CARUB_v10023430mg [Caps... 117 2e-24
ref|XP_006411179.1| hypothetical protein EUTSA_v10016823mg [Eutr... 117 2e-24
ref|XP_006410933.1| hypothetical protein EUTSA_v10016799mg [Eutr... 117 2e-24
>ref|XP_004240770.1| PREDICTED: auxin-induced protein 5NG4-like [Solanum lycopersicum]
Length = 364
Score = 133 bits (335), Expect = 2e-29
Identities = 63/108 (58%), Positives = 79/108 (73%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL+ GP IGLPWT H + ++ D + P+KGA+ I AGC ++ FY LQAITLK
Sbjct: 151 MIMTLVGGPTIGLPWTRHDHINIIPSSTTDLQ-PIKGALFIAAGCICWACFYNLQAITLK 209
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLT LIC+ GAL GT+LT +VERGNTS+W + WD L+YVY
Sbjct: 210 TYPASLSLTCLICSAGALQGTLLTLVVERGNTSIWAIQWDTTFLSYVY 257
>ref|XP_006343754.1| PREDICTED: WAT1-related protein At2g39510-like [Solanum tuberosum]
Length = 366
Score = 132 bits (331), Expect = 6e-29
Identities = 65/108 (60%), Positives = 80/108 (74%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTLI GP IGLPWT H + ++P D + P+KGA+ I AGC ++ FY LQAITLK
Sbjct: 151 MIMTLIGGPTIGLPWTRH-HLNIIPSSPTDLQ-PIKGALFIAAGCICWACFYNLQAITLK 208
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLT LIC+ GAL GT+LT +VERGNTS+W + WD L+YVY
Sbjct: 209 TYPATLSLTCLICSAGALQGTLLTLVVERGNTSIWAIQWDTSFLSYVY 256
>gb|EOY18240.1| Nodulin MtN21 /EamA-like transporter family protein, putative
[Theobroma cacao]
Length = 394
Score = 128 bits (322), Expect = 7e-28
Identities = 61/108 (56%), Positives = 81/108 (75%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL++GPI+ LPWT T++ H ST K+DP+KGA+MI AGC ++ F ILQAITLK
Sbjct: 151 MIMTLVNGPILPLPWTKVTNE-HQSTVSATKDDPLKGALMILAGCVCWACFVILQAITLK 209
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
SYPA LSLT L+C +GA+ GT++ ++E GN + W + WD+KL A VY
Sbjct: 210 SYPAELSLTTLVCFMGAIEGTIVALVMEGGNAAAWSIHWDSKLFAAVY 257
>gb|EPS61790.1| hypothetical protein M569_13004 [Genlisea aurea]
Length = 267
Score = 128 bits (321), Expect = 9e-28
Identities = 61/111 (54%), Positives = 77/111 (69%), Gaps = 2/111 (1%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPN--DKEDPVKGAIMITAGCFGYSLFYILQAIT 174
MIMTL G IIGLPW HQ S + + + VKGA+MI+AGC YS F++LQ+IT
Sbjct: 149 MIMTLYRGSIIGLPWAHQNSGQPGSPSASAATHSEAVKGALMISAGCLSYSTFFVLQSIT 208
Query: 175 LKSYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVYG 327
L+SYPAG+SL ALIC +G+L V+ + ER NTS+W LGW+ KLL Y YG
Sbjct: 209 LESYPAGMSLAALICFVGSLEAGVVALIAERNNTSIWALGWNMKLLGYAYG 259
>gb|EOY18239.1| Nodulin MtN21 /EamA-like transporter family protein, putative
[Theobroma cacao]
Length = 436
Score = 125 bits (314), Expect = 6e-27
Identities = 59/108 (54%), Positives = 79/108 (73%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL++GPI+ LPWT + + H ST K+DP+KGA+MI AGC ++ F ILQAITLK
Sbjct: 151 MIMTLVNGPILPLPWT-KVKNEHQSTVSATKDDPLKGALMILAGCVCWACFVILQAITLK 209
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
SYPA LSLT L+C +GA+ GT++ ++E GN + W + WD+KL A Y
Sbjct: 210 SYPAELSLTTLVCFMGAIEGTIVALVMEGGNAAAWSIHWDSKLFAAAY 257
>ref|XP_006436050.1| hypothetical protein CICLE_v10031857mg [Citrus clementina]
gi|568866307|ref|XP_006486498.1| PREDICTED: WAT1-related
protein At2g39510-like [Citrus sinensis]
gi|557538246|gb|ESR49290.1| hypothetical protein
CICLE_v10031857mg [Citrus clementina]
Length = 375
Score = 124 bits (311), Expect = 1e-26
Identities = 57/108 (52%), Positives = 79/108 (73%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M MTLI GP++ LPWT+ + +++N + + P+KGA+MIT GCF ++ F +LQAITLK
Sbjct: 151 MFMTLIKGPVLDLPWTNHNYHQESTSN-SSVQSPIKGALMITIGCFSWAGFMVLQAITLK 209
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
SYP LSLTALIC +G + GT++ +ERGN +VW + D+KLLA VY
Sbjct: 210 SYPTELSLTALICLMGTIEGTIVALFLERGNAAVWSIHLDSKLLAAVY 257
>ref|XP_002315243.2| hypothetical protein POPTR_0010s21630g [Populus trichocarpa]
gi|550330315|gb|EEF01414.2| hypothetical protein
POPTR_0010s21630g [Populus trichocarpa]
Length = 381
Score = 123 bits (308), Expect = 3e-26
Identities = 62/108 (57%), Positives = 77/108 (71%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTL+ G + LPWT + +D HAST K+DP+KGA+MIT GC ++ F ILQAITLK
Sbjct: 153 MLMTLVKGTQLDLPWT-KGYDQHASTGGLTKQDPIKGALMITTGCACWASFIILQAITLK 211
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
SYP LSLTA IC +G + GTVL ++ERGN S W + D KLLA VY
Sbjct: 212 SYPVELSLTAWICFMGTIEGTVLAVVMERGNPSAWSIALDYKLLAAVY 259
>ref|XP_004302750.1| PREDICTED: auxin-induced protein 5NG4-like [Fragaria vesca subsp.
vesca]
Length = 365
Score = 121 bits (304), Expect = 8e-26
Identities = 57/108 (52%), Positives = 76/108 (70%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL+ GP++ L WT T + TN D +KGA+MITAGCF ++ F ILQ++TL+
Sbjct: 153 MIMTLVKGPLLKLFWTRATTSNQQQTNGVDIHSSIKGALMITAGCFCWACFMILQSMTLR 212
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSL+A IC +G L GT + ++ERGN +VW + WD KLLA VY
Sbjct: 213 TYPAELSLSAWICLMGTLEGTAVALVMERGNAAVWAIHWDGKLLAAVY 260
>ref|XP_006436052.1| hypothetical protein CICLE_v10031808mg [Citrus clementina]
gi|568865395|ref|XP_006486061.1| PREDICTED: WAT1-related
protein At2g39510-like [Citrus sinensis]
gi|557538248|gb|ESR49292.1| hypothetical protein
CICLE_v10031808mg [Citrus clementina]
Length = 383
Score = 121 bits (303), Expect = 1e-25
Identities = 60/111 (54%), Positives = 77/111 (69%), Gaps = 2/111 (1%)
Frame = +1
Query: 1 MIMTLISGPIIGLPW--THQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAIT 174
M MTL GPI+ PW H+ ++ N++ED KGA++I AGCF +S F ILQA
Sbjct: 154 MCMTLFKGPILKFPWKQVRLLHNQLETSIQNNEEDLTKGALLIAAGCFAWSCFIILQAFL 213
Query: 175 LKSYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVYG 327
LKSYPA LSLTALIC + ++ GT+LT +ERGNT +W L +DAKLLA +YG
Sbjct: 214 LKSYPAELSLTALICFMSSVEGTILTLAIERGNTGIWLLHFDAKLLAVIYG 264
>ref|XP_002274909.2| PREDICTED: auxin-induced protein 5NG4-like [Vitis vinifera]
Length = 396
Score = 120 bits (300), Expect = 2e-25
Identities = 56/108 (51%), Positives = 74/108 (68%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTL+ GP+I L WT ++ + D +KG++MITAGCF ++ F ILQAITLK
Sbjct: 157 MLMTLVKGPVIELIWTKGRNNQGVKGGGTNIHDSIKGSLMITAGCFSWACFIILQAITLK 216
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC LG GT++ ++ERG TSVW + WD K LA +Y
Sbjct: 217 TYPAELSLTAWICLLGTAQGTIVALVMERGKTSVWSIHWDTKFLAALY 264
>emb|CBI32681.3| unnamed protein product [Vitis vinifera]
Length = 383
Score = 120 bits (300), Expect = 2e-25
Identities = 56/108 (51%), Positives = 74/108 (68%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTL+ GP+I L WT ++ + D +KG++MITAGCF ++ F ILQAITLK
Sbjct: 163 MLMTLVKGPVIELIWTKGRNNQGVKGGGTNIHDSIKGSLMITAGCFSWACFIILQAITLK 222
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC LG GT++ ++ERG TSVW + WD K LA +Y
Sbjct: 223 TYPAELSLTAWICLLGTAQGTIVALVMERGKTSVWSIHWDTKFLAALY 270
>gb|EXB67895.1| Auxin-induced protein 5NG4 [Morus notabilis]
Length = 357
Score = 119 bits (297), Expect = 5e-25
Identities = 57/108 (52%), Positives = 73/108 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MT++ GPI+ L WT T A + +KG++MIT GCF ++ F ILQAITLK
Sbjct: 132 MVMTVMKGPILDLFWTRGTRVDEARSGGISLSHSIKGSLMITVGCFSWACFMILQAITLK 191
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC LG L GT + ++ERGN SVW L WD KL+A +Y
Sbjct: 192 TYPAELSLTAWICLLGTLEGTAVALVMERGNASVWSLKWDTKLMAALY 239
>ref|XP_006410931.1| hypothetical protein EUTSA_v10017814mg [Eutrema salsugineum]
gi|557112100|gb|ESQ52384.1| hypothetical protein
EUTSA_v10017814mg [Eutrema salsugineum]
Length = 281
Score = 119 bits (297), Expect = 5e-25
Identities = 56/108 (51%), Positives = 74/108 (68%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTL+ GP++ L WT + S +T D + +KGAI +T GCF Y+ F+ILQAITLK
Sbjct: 54 MVMTLVKGPVLNLFWTKEP--SAQNTAGTDIHNSIKGAIFVTIGCFSYACFHILQAITLK 111
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
YPA LSLTALIC +G + G ++ ++ERGN S W +GWD KLL Y
Sbjct: 112 IYPAELSLTALICLMGTIEGAIVALVMERGNLSAWTIGWDMKLLTVSY 159
>ref|XP_004307548.1| PREDICTED: auxin-induced protein 5NG4-like [Fragaria vesca subsp.
vesca]
Length = 350
Score = 119 bits (297), Expect = 5e-25
Identities = 59/109 (54%), Positives = 74/109 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTL++GP++ LPWT T H T +DP+KGA+MITAGCF ++ F ILQA+TLK
Sbjct: 153 MLMTLVNGPMLTLPWTRST-SHHQYTGSTASQDPIKGALMITAGCFCWASFMILQAVTLK 211
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVYG 327
YPA LSLTA IC +G L GTV +E NT+ W + D KLL VYG
Sbjct: 212 VYPAELSLTAWICLMGTLEGTVAAVAIEWHNTAAWAIHLDYKLLPAVYG 260
>ref|XP_006429015.1| hypothetical protein CICLE_v10012001mg [Citrus clementina]
gi|568854258|ref|XP_006480748.1| PREDICTED: WAT1-related
protein At2g37460-like [Citrus sinensis]
gi|557531072|gb|ESR42255.1| hypothetical protein
CICLE_v10012001mg [Citrus clementina]
Length = 370
Score = 117 bits (294), Expect = 1e-24
Identities = 56/108 (51%), Positives = 73/108 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MTLI GPI+ L WT + ++ + +KGA+MITAGCF +S F ILQAITLK
Sbjct: 152 MVMTLIKGPILELFWTKGAENHGHGSSGTTTHNSIKGALMITAGCFSWSFFIILQAITLK 211
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC G + GT+ ++ERG S+W + WD KL+A VY
Sbjct: 212 AYPAELSLTAWICFFGTVEGTLAALIMERGKASIWAIHWDTKLVASVY 259
>ref|XP_004236759.1| PREDICTED: auxin-induced protein 5NG4-like [Solanum lycopersicum]
Length = 376
Score = 117 bits (294), Expect = 1e-24
Identities = 60/106 (56%), Positives = 74/106 (69%), Gaps = 4/106 (3%)
Frame = +1
Query: 4 IMTLISGPIIGLPWTHQTHDSHASTNPN----DKEDPVKGAIMITAGCFGYSLFYILQAI 171
IM+L+ GPIIGLPWT H S+ T N ++ +PVKGAI I AGCF ++ FY LQAI
Sbjct: 157 IMSLVGGPIIGLPWTK--HHSNVPTTKNVVSPNELNPVKGAIFIAAGCFCWACFYNLQAI 214
Query: 172 TLKSYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKL 309
TLK Y AG+SLT+LIC GAL GT LT LV+RGN +W + W + L
Sbjct: 215 TLKKYQAGMSLTSLICMSGALQGTALTLLVQRGNFGIWSIKWGSSL 260
>ref|XP_006365719.1| PREDICTED: WAT1-related protein At2g39510-like [Solanum tuberosum]
Length = 377
Score = 117 bits (293), Expect = 2e-24
Identities = 59/105 (56%), Positives = 74/105 (70%), Gaps = 3/105 (2%)
Frame = +1
Query: 4 IMTLISGPIIGLPWTHQTHDSHASTN---PNDKEDPVKGAIMITAGCFGYSLFYILQAIT 174
IMTL+ GP IGLPWT ++ +TN PN+ +P+KGAI I AGCF ++ FY LQAIT
Sbjct: 157 IMTLVGGPTIGLPWTKHHNNVPTTTNVVSPNEL-NPLKGAIFIAAGCFCWACFYNLQAIT 215
Query: 175 LKSYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKL 309
LK Y AG+SLT+LIC GAL GT LT LV+RGN +W + W + L
Sbjct: 216 LKKYQAGMSLTSLICMSGALQGTALTLLVQRGNFGIWSIHWGSSL 260
>ref|XP_006294412.1| hypothetical protein CARUB_v10023430mg [Capsella rubella]
gi|482563120|gb|EOA27310.1| hypothetical protein
CARUB_v10023430mg [Capsella rubella]
Length = 380
Score = 117 bits (293), Expect = 2e-24
Identities = 55/108 (50%), Positives = 73/108 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL+ GP++ L WT S +T D +KGA+++T GCF Y+ F ILQA+TL+
Sbjct: 153 MIMTLVKGPVLDLFWTKGI--SSKNTAGTDIHSAIKGAVLVTIGCFSYACFMILQAVTLR 210
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC +G L GTV+ ++E+GN S W +GWD KLL Y
Sbjct: 211 TYPAELSLTAWICLMGTLEGTVVALVMEKGNPSAWAIGWDTKLLTSTY 258
>ref|XP_006411179.1| hypothetical protein EUTSA_v10016823mg [Eutrema salsugineum]
gi|557112348|gb|ESQ52632.1| hypothetical protein
EUTSA_v10016823mg [Eutrema salsugineum]
Length = 371
Score = 117 bits (292), Expect = 2e-24
Identities = 53/109 (48%), Positives = 74/109 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
M+MT++ GP+I LPWT+ + S+NP K D KGA++I GC ++ F LQAITLK
Sbjct: 146 MLMTVVKGPLIPLPWTNPNENHQDSSNPGVKPDLTKGALLIATGCICWAAFVNLQAITLK 205
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVYG 327
SYP LSLTA IC +G++ T++ +ERGN + W + D+KLLA +YG
Sbjct: 206 SYPVELSLTAYICLMGSIESTIVALFIERGNPAAWAIQLDSKLLAAIYG 254
>ref|XP_006410933.1| hypothetical protein EUTSA_v10016799mg [Eutrema salsugineum]
gi|557112102|gb|ESQ52386.1| hypothetical protein
EUTSA_v10016799mg [Eutrema salsugineum]
Length = 380
Score = 117 bits (292), Expect = 2e-24
Identities = 53/108 (49%), Positives = 73/108 (67%)
Frame = +1
Query: 1 MIMTLISGPIIGLPWTHQTHDSHASTNPNDKEDPVKGAIMITAGCFGYSLFYILQAITLK 180
MIMTL+ GP++ L WT + S +TN D +KGA+++T GCF Y+ F ILQA+TL+
Sbjct: 153 MIMTLVKGPVLDLFWTKGS--SAQNTNGTDIHSAIKGAVLVTIGCFSYACFMILQAVTLR 210
Query: 181 SYPAGLSLTALICTLGALLGTVLTFLVERGNTSVWGLGWDAKLLAYVY 324
+YPA LSLTA IC +G + GT + ++E+GN W +GWD KLL Y
Sbjct: 211 TYPAELSLTAWICLMGTIEGTAVALVMEKGNPGAWAIGWDTKLLTATY 258