BLASTX nr result
ID: Rehmannia22_contig00035788
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Rehmannia22_contig00035788 (351 letters) Database: ./nr 37,332,560 sequences; 13,225,080,153 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EOX94092.1| Gag protease polyprotein [Theobroma cacao] 174 1e-41 gb|EOY14138.1| Uncharacterized protein TCM_033423 [Theobroma cacao] 171 7e-41 gb|EOY13858.1| Uncharacterized protein TCM_032543 [Theobroma cacao] 171 9e-41 emb|CAN81132.1| hypothetical protein VITISV_009934 [Vitis vinifera] 170 2e-40 emb|CAN61139.1| hypothetical protein VITISV_009489 [Vitis vinifera] 169 3e-40 gb|EOY16995.1| Uncharacterized protein TCM_036100 [Theobroma cacao] 168 6e-40 emb|CAN71472.1| hypothetical protein VITISV_040055 [Vitis vinifera] 168 6e-40 emb|CAN64875.1| hypothetical protein VITISV_019676 [Vitis vinifera] 168 6e-40 emb|CAN77351.1| hypothetical protein VITISV_004008 [Vitis vinifera] 168 8e-40 emb|CAN77801.1| hypothetical protein VITISV_031477 [Vitis vinifera] 167 1e-39 emb|CAC44142.1| putative polyprotein [Cicer arietinum] 167 1e-39 gb|EOY03692.1| Gag protease polyprotein [Theobroma cacao] 167 1e-39 gb|EOX94203.1| DNA/RNA polymerases superfamily protein [Theobrom... 166 2e-39 gb|EOX93994.1| DNA/RNA polymerases superfamily protein [Theobrom... 166 2e-39 emb|CAA73042.1| polyprotein [Ananas comosus] 166 4e-39 ref|XP_004515382.1| PREDICTED: uncharacterized protein LOC101499... 165 5e-39 gb|EOY21657.1| DNA/RNA polymerases superfamily protein [Theobrom... 165 7e-39 gb|EOY00215.1| DNA/RNA polymerases superfamily protein [Theobrom... 164 9e-39 emb|CAN63702.1| hypothetical protein VITISV_025813 [Vitis vinifera] 162 3e-38 gb|EMJ22433.1| hypothetical protein PRUPE_ppb019121mg [Prunus pe... 162 4e-38 >gb|EOX94092.1| Gag protease polyprotein [Theobroma cacao] Length = 269 Score = 174 bits (441), Expect = 1e-41 Identities = 84/114 (73%), Positives = 97/114 (85%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ R NDAIWVIVDRLTKSAHF+ V T+++++ AQLYI++IV LHGVPVSIVSDRD Sbjct: 79 GLPRTQRGNDAIWVIVDRLTKSAHFLAVHSTYSIEKLAQLYIDEIVRLHGVPVSIVSDRD 138 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW A+ TKLKFSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 139 PRFTSRFWLKFQEALGTKLKFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 192 >gb|EOY14138.1| Uncharacterized protein TCM_033423 [Theobroma cacao] Length = 809 Score = 171 bits (434), Expect = 7e-41 Identities = 81/114 (71%), Positives = 96/114 (84%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ DAIWVIVDRLTKSAHF+ + T++++R A+LYI++IV LHGVPVSIVSDRD Sbjct: 684 GLPRTQSGKDAIWVIVDRLTKSAHFLAIHSTYSIERLARLYIDEIVRLHGVPVSIVSDRD 743 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW H A+ TKL+FSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 744 PRFTSRFWPKFHEALGTKLRFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 797 >gb|EOY13858.1| Uncharacterized protein TCM_032543 [Theobroma cacao] Length = 189 Score = 171 bits (433), Expect = 9e-41 Identities = 81/113 (71%), Positives = 96/113 (84%) Frame = -1 Query: 345 LPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRDP 166 LPR+ R DAIWVIVDRLTKSAHF+ V T+++++ AQLYI++IV LHGVP+SIVSDRDP Sbjct: 7 LPRTQRGKDAIWVIVDRLTKSAHFLAVHSTYSIEKLAQLYIDEIVRLHGVPISIVSDRDP 66 Query: 165 RFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 RFTS+FW A++TKLKFSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 67 RFTSRFWPKFQEALETKLKFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 119 >emb|CAN81132.1| hypothetical protein VITISV_009934 [Vitis vinifera] Length = 730 Score = 170 bits (431), Expect = 2e-40 Identities = 79/115 (68%), Positives = 97/115 (84%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLPR++ N+AIWVIVDRLTKSAHF+P+++ F+MD A LYI++IV +HGVP+SIVSDR Sbjct: 550 TGLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFSMDHLASLYIKEIVRMHGVPLSIVSDR 609 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 DP FTS+FW SL A+ TKL FSTA+HPQTDGQS+R IQ LED+LR VLDL G+ Sbjct: 610 DPHFTSRFWHSLQKALSTKLSFSTAFHPQTDGQSDRVIQVLEDLLRACVLDLKGN 664 >emb|CAN61139.1| hypothetical protein VITISV_009489 [Vitis vinifera] Length = 984 Score = 169 bits (429), Expect = 3e-40 Identities = 77/114 (67%), Positives = 97/114 (85%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR++ N+AIWVIVDRLTKSAHF+P+++ F++DR A LY+++IV +HGVPVSIVSDRD Sbjct: 635 GLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFSLDRLASLYVKEIVRMHGVPVSIVSDRD 694 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW SL ++ TKL FSTA+HPQTDGQSER IQ LED+ R +LDL G+ Sbjct: 695 PRFTSRFWHSLQKSLGTKLSFSTAFHPQTDGQSERVIQVLEDLFRACILDLQGN 748 >gb|EOY16995.1| Uncharacterized protein TCM_036100 [Theobroma cacao] Length = 160 Score = 168 bits (426), Expect = 6e-40 Identities = 80/114 (70%), Positives = 95/114 (83%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ DAIWVIVDRLTKSAHF+ + TF+++R A+LYI+++V LHGVPVSIVSDRD Sbjct: 6 GLPRTQSGKDAIWVIVDRLTKSAHFLAIHSTFSIERLARLYIDEVVRLHGVPVSIVSDRD 65 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW A+ TKL+FSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 66 PRFTSRFWLKFQEALGTKLRFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 119 >emb|CAN71472.1| hypothetical protein VITISV_040055 [Vitis vinifera] Length = 374 Score = 168 bits (426), Expect = 6e-40 Identities = 77/114 (67%), Positives = 97/114 (85%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR++ N+AIWVIVDRLTKSAHF+P+++ F++DR A LY+++IV +HGVPVSIVSDRD Sbjct: 49 GLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFSLDRLASLYVKEIVRMHGVPVSIVSDRD 108 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW SL A+ TKL FSTA+HPQTDGQSE IQ LED+LR +L+L G+ Sbjct: 109 PRFTSRFWHSLQKALGTKLSFSTAFHPQTDGQSEMVIQVLEDLLRACILELQGN 162 >emb|CAN64875.1| hypothetical protein VITISV_019676 [Vitis vinifera] Length = 386 Score = 168 bits (426), Expect = 6e-40 Identities = 77/114 (67%), Positives = 97/114 (85%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR++ N+AIWVIVDRLTKSAHF+P+++ F++DR A L++++IV +HGVPVSIVSDRD Sbjct: 49 GLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFSLDRLASLHVKEIVRMHGVPVSIVSDRD 108 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 P FTS+FW SL A+ TKL FSTA+HPQTDGQSER IQ LED+LR +LDL G+ Sbjct: 109 PHFTSRFWHSLQKALGTKLSFSTAFHPQTDGQSERVIQVLEDLLRACILDLQGN 162 >emb|CAN77351.1| hypothetical protein VITISV_004008 [Vitis vinifera] Length = 869 Score = 168 bits (425), Expect = 8e-40 Identities = 79/115 (68%), Positives = 95/115 (82%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLPR++ N+AIWVIVDRLTKSAHF+P+++ F+M+R LYI++IV +HGVPVSIVSDR Sbjct: 589 TGLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFSMNRLTSLYIKEIVRMHGVPVSIVSDR 648 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 DPRFTS+FW SL A+ TKL FST +HPQTDGQSER IQ LED+LR LDL S Sbjct: 649 DPRFTSRFWHSLQKALGTKLSFSTVFHPQTDGQSERVIQVLEDLLRACALDLKAS 703 >emb|CAN77801.1| hypothetical protein VITISV_031477 [Vitis vinifera] Length = 855 Score = 167 bits (424), Expect = 1e-39 Identities = 77/115 (66%), Positives = 96/115 (83%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLPR++ N+AIWVIVDRLTKSAHF+P+++ F +DR A LY++ IV +HGVPVSI+SDR Sbjct: 529 TGLPRTLGGNNAIWVIVDRLTKSAHFLPMKVNFYLDRLASLYVKXIVRMHGVPVSIMSDR 588 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 DPRFTS+FW SL + TKL FSTA+HPQTDGQSE+ IQ LED+LR +LDL G+ Sbjct: 589 DPRFTSRFWHSLQKDLGTKLSFSTAFHPQTDGQSEKVIQVLEDLLRACILDLQGN 643 >emb|CAC44142.1| putative polyprotein [Cicer arietinum] Length = 655 Score = 167 bits (423), Expect = 1e-39 Identities = 79/115 (68%), Positives = 97/115 (84%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLP++ RKND+IWVIVDRLTKSAHF+PVR T+ +D+ ++YI +IV LHGVP SIVSDR Sbjct: 407 TGLPKTRRKNDSIWVIVDRLTKSAHFLPVRTTYKVDQLTEIYIAEIVRLHGVPSSIVSDR 466 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 DP+FTS FW +LH A+ TKL+ S+AYHPQTDGQ+ERT Q+LED+LR VLD GS Sbjct: 467 DPKFTSHFWGALHEALGTKLRLSSAYHPQTDGQTERTNQSLEDLLRACVLDDRGS 521 >gb|EOY03692.1| Gag protease polyprotein [Theobroma cacao] Length = 689 Score = 167 bits (423), Expect = 1e-39 Identities = 80/114 (70%), Positives = 95/114 (83%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ R DAIWVIVDRLTKSAHF+ V T+++++ AQLYI++IV LHGVPV IVSD+D Sbjct: 488 GLPRTQRGKDAIWVIVDRLTKSAHFLAVHSTYSIEKLAQLYIDEIVRLHGVPVFIVSDQD 547 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW A+ TKLKFSTA+HPQTDGQSERTIQTL+DMLR V+D GS Sbjct: 548 PRFTSRFWPKFQEALGTKLKFSTAFHPQTDGQSERTIQTLKDMLRACVIDFIGS 601 >gb|EOX94203.1| DNA/RNA polymerases superfamily protein [Theobroma cacao] Length = 1336 Score = 166 bits (421), Expect = 2e-39 Identities = 80/114 (70%), Positives = 94/114 (82%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GL R+ R D IWVIVD+LTKSAHF+ V T+++++ AQLYI++IV LHGVPVSIVSDRD Sbjct: 1084 GLSRTQRGKDVIWVIVDQLTKSAHFLAVHSTYSIEKLAQLYIDEIVRLHGVPVSIVSDRD 1143 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW A+ TKLKFSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 1144 PRFTSRFWPKFQEALGTKLKFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 1197 >gb|EOX93994.1| DNA/RNA polymerases superfamily protein [Theobroma cacao] Length = 811 Score = 166 bits (421), Expect = 2e-39 Identities = 77/114 (67%), Positives = 95/114 (83%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ DAIWVIVDRLTKSAHF+ + T++++R A+LYI+++V LHGVP+SIVSDRD Sbjct: 580 GLPRTQSGKDAIWVIVDRLTKSAHFLAIHSTYSIERLARLYIDEVVRLHGVPISIVSDRD 639 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+FW A+ TKL+FST++HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 640 PRFTSRFWPKFQEALGTKLRFSTSFHPQTDGQSERTIQTLEDMLRACVIDFIGS 693 >emb|CAA73042.1| polyprotein [Ananas comosus] Length = 871 Score = 166 bits (419), Expect = 4e-39 Identities = 80/114 (70%), Positives = 93/114 (81%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLPRS +DAIWVIVDRLTKSAHFIP+ T+T +R AQ+Y+++IV LHGVP SIVSDR Sbjct: 605 TGLPRSQAGHDAIWVIVDRLTKSAHFIPIHTTWTGERLAQVYLDEIVRLHGVPTSIVSDR 664 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDG 10 D RF S FW SL A+ T+L FSTA+HPQ+DGQSERTIQTLEDMLR V+D G Sbjct: 665 DTRFVSHFWRSLQDALGTRLDFSTAFHPQSDGQSERTIQTLEDMLRACVIDFQG 718 >ref|XP_004515382.1| PREDICTED: uncharacterized protein LOC101499978 [Cicer arietinum] Length = 1352 Score = 165 bits (418), Expect = 5e-39 Identities = 75/114 (65%), Positives = 98/114 (85%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ ++ D+IWVIVDRLTKSAHFIP+ +T++M+R A++YI++IV LHG+P SIVSDRD Sbjct: 846 GLPRTPKRYDSIWVIVDRLTKSAHFIPINITYSMERLAEIYIKEIVKLHGIPSSIVSDRD 905 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTSKFW+ L A+ T L+ S+AYHPQTDGQ+ERT Q+LED+LR VL+ +GS Sbjct: 906 PRFTSKFWQGLQRALGTNLRMSSAYHPQTDGQTERTNQSLEDLLRACVLEQNGS 959 >gb|EOY21657.1| DNA/RNA polymerases superfamily protein [Theobroma cacao] Length = 1188 Score = 165 bits (417), Expect = 7e-39 Identities = 78/114 (68%), Positives = 94/114 (82%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ DAIWVIVDRLTKSAHF+ + T++++R A+LYI++IV LHGVP+SIVSDRD Sbjct: 987 GLPRTQSGKDAIWVIVDRLTKSAHFLAIHSTYSIERLARLYIDEIVRLHGVPISIVSDRD 1046 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 PRFTS+ W A+ TKL+FSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 1047 PRFTSRLWLKFQEALGTKLRFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 1100 >gb|EOY00215.1| DNA/RNA polymerases superfamily protein [Theobroma cacao] Length = 1537 Score = 164 bits (416), Expect = 9e-39 Identities = 79/114 (69%), Positives = 94/114 (82%) Frame = -1 Query: 348 GLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRD 169 GLPR+ DAIWVIVDRLTKSAHF+ + T++++R A+LYI++IV LHGVPVSIVSDRD Sbjct: 1179 GLPRTQSGKDAIWVIVDRLTKSAHFLAIHSTYSIERLARLYIDEIVRLHGVPVSIVSDRD 1238 Query: 168 PRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDGS 7 RFTS+FW A+ TKL+FSTA+HPQTDGQSERTIQTLEDMLR V+D GS Sbjct: 1239 LRFTSRFWPKFQEALGTKLRFSTAFHPQTDGQSERTIQTLEDMLRACVIDFIGS 1292 >emb|CAN63702.1| hypothetical protein VITISV_025813 [Vitis vinifera] Length = 934 Score = 162 bits (411), Expect = 3e-38 Identities = 75/112 (66%), Positives = 93/112 (83%) Frame = -1 Query: 351 TGLPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDR 172 TGLPR++ N+ IWVIVD+LTKSAHF+P+++ F+MDR A LYI++I+ +HGVP+SIVSDR Sbjct: 711 TGLPRTLEGNNVIWVIVDQLTKSAHFLPMKVNFSMDRLASLYIKEIMRMHGVPISIVSDR 770 Query: 171 DPRFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDL 16 DPRFTS+FW SL + TKL FSTA+H QTDGQSER IQ LED+LR LDL Sbjct: 771 DPRFTSRFWHSLQKTLGTKLSFSTAFHLQTDGQSERVIQVLEDLLRACALDL 822 >gb|EMJ22433.1| hypothetical protein PRUPE_ppb019121mg [Prunus persica] Length = 552 Score = 162 bits (410), Expect = 4e-38 Identities = 75/112 (66%), Positives = 94/112 (83%) Frame = -1 Query: 345 LPRSVRKNDAIWVIVDRLTKSAHFIPVRMTFTMDRFAQLYIEKIVSLHGVPVSIVSDRDP 166 LPR+ K+D +WVIVDRLTKSAHF+PVR +++++ A+++I++IV LHGVPVSIVSDRDP Sbjct: 213 LPRTQSKHDGVWVIVDRLTKSAHFLPVRANYSLNKLAKIFIDEIVRLHGVPVSIVSDRDP 272 Query: 165 RFTSKFWESLHAAMKTKLKFSTAYHPQTDGQSERTIQTLEDMLRTVVLDLDG 10 RFTS+FW L+ A T+L+FSTA+HPQTDGQSERTIQTLEDMLR L G Sbjct: 273 RFTSRFWTKLNEAFGTQLQFSTAFHPQTDGQSERTIQTLEDMLRACALQFRG 324