BLASTX nr result
ID: Rehmannia22_contig00014514
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia22_contig00014514
(931 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EOY24538.1| ACC synthase 10 isoform 2 [Theobroma cacao] 418 e-114
gb|EOY24537.1| ACC synthase 10 isoform 1 [Theobroma cacao] 418 e-114
gb|ABY55587.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa ... 410 e-112
gb|AAQ88100.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa ... 410 e-112
ref|XP_002509645.1| acc synthase, putative [Ricinus communis] gi... 409 e-111
ref|XP_004245687.1| PREDICTED: probable aminotransferase ACS10-l... 408 e-111
gb|EMJ11078.1| hypothetical protein PRUPE_ppa003908mg [Prunus pe... 407 e-111
ref|XP_006363709.1| PREDICTED: probable aminotransferase ACS10-l... 407 e-111
ref|XP_004298716.1| PREDICTED: probable aminotransferase ACS10-l... 404 e-110
ref|XP_006440457.1| hypothetical protein CICLE_v10019580mg [Citr... 404 e-110
gb|ABY55589.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa ... 404 e-110
ref|XP_002304448.1| 1-aminocyclopropane-1-carboxylate synthase f... 404 e-110
ref|XP_002281504.2| PREDICTED: probable aminotransferase ACS10-l... 403 e-110
ref|XP_006477323.1| PREDICTED: probable aminotransferase ACS10-l... 403 e-110
emb|CBI23558.3| unnamed protein product [Vitis vinifera] 396 e-108
gb|ABY55588.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa ... 395 e-107
ref|XP_002268932.1| PREDICTED: probable aminotransferase ACS12-l... 384 e-104
gb|EXC17820.1| putative aminotransferase ACS10 [Morus notabilis] 384 e-104
emb|CAN66821.1| hypothetical protein VITISV_003497 [Vitis vinifera] 382 e-104
gb|AAG16977.1|AF184076_1 ACC synthase [Prunus armeniaca] 377 e-102
>gb|EOY24538.1| ACC synthase 10 isoform 2 [Theobroma cacao]
Length = 458
Score = 418 bits (1075), Expect = e-114
Identities = 203/277 (73%), Positives = 238/277 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEII VPCRS DNF+LSITALD AFNQAKKRGL+VRGIIISNPSNPVG L RET
Sbjct: 180 KWRTGVEIIHVPCRSADNFNLSITALDIAFNQAKKRGLKVRGIIISNPSNPVGNLLSRET 239
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHI+SNE+L AG THG++EFVSMAEI++ E++DR RVH++YGLS DLS
Sbjct: 240 LYNLLDFAREKNIHIVSNEIL-AGCTHGNEEFVSMAEIVDLEDVDRKRVHLVYGLSKDLS 298
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+FNE V AAKKLTRFSS+S PTQ LLISMLSD KFV+ + + ERL R
Sbjct: 299 LPGFRVGVIYSFNEEVLAAAKKLTRFSSISAPTQRLLISMLSDTKFVQTFITINRERLHR 358
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY++F GLKKLGIEC+KS GGFYCWADMSGLI SYSEKGELELWDKLLN+AK+N GS
Sbjct: 359 MYVQFVEGLKKLGIECIKSSGGFYCWADMSGLISSYSEKGELELWDKLLNIAKVNVTPGS 418
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCR 101
C+C+EPGWF FCFA+L+E+DI V+ RI +VS+TC+
Sbjct: 419 CCHCIEPGWFRFCFATLTEKDIPVVMERIQKVSETCK 455
>gb|EOY24537.1| ACC synthase 10 isoform 1 [Theobroma cacao]
Length = 544
Score = 418 bits (1075), Expect = e-114
Identities = 203/277 (73%), Positives = 238/277 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEII VPCRS DNF+LSITALD AFNQAKKRGL+VRGIIISNPSNPVG L RET
Sbjct: 266 KWRTGVEIIHVPCRSADNFNLSITALDIAFNQAKKRGLKVRGIIISNPSNPVGNLLSRET 325
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHI+SNE+L AG THG++EFVSMAEI++ E++DR RVH++YGLS DLS
Sbjct: 326 LYNLLDFAREKNIHIVSNEIL-AGCTHGNEEFVSMAEIVDLEDVDRKRVHLVYGLSKDLS 384
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+FNE V AAKKLTRFSS+S PTQ LLISMLSD KFV+ + + ERL R
Sbjct: 385 LPGFRVGVIYSFNEEVLAAAKKLTRFSSISAPTQRLLISMLSDTKFVQTFITINRERLHR 444
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY++F GLKKLGIEC+KS GGFYCWADMSGLI SYSEKGELELWDKLLN+AK+N GS
Sbjct: 445 MYVQFVEGLKKLGIECIKSSGGFYCWADMSGLISSYSEKGELELWDKLLNIAKVNVTPGS 504
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCR 101
C+C+EPGWF FCFA+L+E+DI V+ RI +VS+TC+
Sbjct: 505 CCHCIEPGWFRFCFATLTEKDIPVVMERIQKVSETCK 541
>gb|ABY55587.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa hybrid cultivar]
Length = 480
Score = 410 bits (1055), Expect = e-112
Identities = 197/278 (70%), Positives = 237/278 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS D F+LSITALDRAFNQAKKRG++VRGIIISNPSNP G L RE+
Sbjct: 202 KWRTGVEIIPVPCRSADKFNLSITALDRAFNQAKKRGVKVRGIIISNPSNPGGSLLTRES 261
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHIISNEL AGST+G +EFVSMAEI++ E+LD+NRVHI+YG+S DLS
Sbjct: 262 LYNLLDFAREKNIHIISNELF-AGSTYGSEEFVSMAEIVDLEDLDQNRVHIVYGISKDLS 320
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++G IY+FN+NV AAKKLTRFSS+S P+Q LLISMLSD KF+ + +E + ERL
Sbjct: 321 LPGFRVGAIYSFNKNVLTAAKKLTRFSSISAPSQRLLISMLSDTKFMHKFIEINRERLRG 380
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ F GLK+LGIEC KS+GGFYCWAD+SGLIRSYSEKGELELWD+LLN+ K+N GS
Sbjct: 381 MYLRFVTGLKQLGIECTKSNGGFYCWADLSGLIRSYSEKGELELWDRLLNVGKLNVTPGS 440
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWF FCF +L+E+DI VI RI +++TC+S
Sbjct: 441 SCHCIEPGWFRFCFTTLTEKDIPVVIERIRNIAETCKS 478
>gb|AAQ88100.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa hybrid cultivar]
Length = 480
Score = 410 bits (1055), Expect = e-112
Identities = 197/278 (70%), Positives = 237/278 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS D F+LSITALDRAFNQAKKRG++VRGIIISNPSNP G L RE+
Sbjct: 202 KWRTGVEIIPVPCRSADKFNLSITALDRAFNQAKKRGVKVRGIIISNPSNPGGSLLTRES 261
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHIISNEL AGST+G +EFVSMAEI++ E+LD+NRVHI+YG+S DLS
Sbjct: 262 LYNLLDFAREKNIHIISNELF-AGSTYGSEEFVSMAEIVDLEDLDQNRVHIVYGISKDLS 320
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++G IY+FN+NV AAKKLTRFSS+S P+Q LLISMLSD KF+ + +E + ERL
Sbjct: 321 LPGFRVGAIYSFNKNVLTAAKKLTRFSSISAPSQRLLISMLSDTKFMHKFIEINRERLRG 380
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ F GLK+LGIEC KS+GGFYCWAD+SGLIRSYSEKGELELWD+LLN+ K+N GS
Sbjct: 381 MYLRFVTGLKQLGIECTKSNGGFYCWADLSGLIRSYSEKGELELWDRLLNVGKLNVTPGS 440
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWF FCF +L+E+DI VI RI +++TC+S
Sbjct: 441 SCHCIEPGWFRFCFTTLTEKDIPVVIERIRNIAETCKS 478
>ref|XP_002509645.1| acc synthase, putative [Ricinus communis] gi|223549544|gb|EEF51032.1|
acc synthase, putative [Ricinus communis]
Length = 556
Score = 409 bits (1050), Expect = e-111
Identities = 197/278 (70%), Positives = 239/278 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGV+IIPVPCRS DNF+LSITALDRAFNQAKKRGL+VRGII SNPS+PVG L +RE
Sbjct: 278 KWRTGVDIIPVPCRSADNFNLSITALDRAFNQAKKRGLKVRGIIFSNPSHPVGNLLNREA 337
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LYSL+DFA EKNIHI+ NE+ AGST+G++EFVSMAE+++ E+ DR+RVHI+Y LS DLS
Sbjct: 338 LYSLLDFAREKNIHIVCNEIF-AGSTYGNEEFVSMAELIDLEDSDRDRVHIVYALSKDLS 396
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G K+GVI++ NENV AAKKLTRFSS+S PT LLISMLSD KFVR I++ + RL +
Sbjct: 397 LPGFKVGVIFSSNENVVAAAKKLTRFSSISAPTHRLLISMLSDIKFVRDIIQTNRNRLQK 456
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ FA GLK+LGI+C+KS+GGFYCWADMS LI SYSEKGELELWDKLLN AK+N+ GS
Sbjct: 457 MYVAFAAGLKQLGIKCIKSNGGFYCWADMSELISSYSEKGELELWDKLLNSAKLNATPGS 516
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWFGFCF +L+ERDI V+ RI ++++TC+S
Sbjct: 517 SCHCIEPGWFGFCFTTLTERDIPVVMDRIRKIAETCKS 554
>ref|XP_004245687.1| PREDICTED: probable aminotransferase ACS10-like [Solanum
lycopersicum]
Length = 548
Score = 408 bits (1048), Expect = e-111
Identities = 198/278 (71%), Positives = 240/278 (86%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNF+LSI ALDRAFNQAKKRGL+VRGIIISNPSNPVG ++ RET
Sbjct: 270 KWRTGVEIIPVPCRSADNFNLSIDALDRAFNQAKKRGLKVRGIIISNPSNPVGNIFSRET 329
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DF TEKNIH+ISNE+L AGST+G++EFVSMAEI+++E+ DR+RVHI+YGLS DLS
Sbjct: 330 LYNLLDFTTEKNIHVISNEIL-AGSTYGNEEFVSMAEIIDSEDFDRSRVHIVYGLSKDLS 388
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+ NENV AAKKLTRFSS+S PTQHL+I MLSD KFV+Q ++ + ERL R
Sbjct: 389 LPGFRVGVIYSCNENVLAAAKKLTRFSSISAPTQHLIIQMLSDAKFVQQFIKKNRERLRR 448
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
M F +GLK+LGIEC +S GGFYCWADMS LIRSY+EKGE+ELWD LLN+AKIN+ GS
Sbjct: 449 MSSLFVSGLKQLGIECTRSSGGFYCWADMSRLIRSYNEKGEIELWDNLLNVAKINATPGS 508
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+CVEPGWF CF++LSE+DI +V+ RI +V + +S
Sbjct: 509 SCHCVEPGWFRLCFSTLSEKDISAVMQRIQKVLELRKS 546
>gb|EMJ11078.1| hypothetical protein PRUPE_ppa003908mg [Prunus persica]
Length = 540
Score = 407 bits (1047), Expect = e-111
Identities = 196/277 (70%), Positives = 239/277 (86%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEII VPCRS D F+LS+TALDRAFNQAKKRGL+VRGIIISNPSNPVG L+ RE+
Sbjct: 262 KWRTGVEIITVPCRSADKFNLSVTALDRAFNQAKKRGLKVRGIIISNPSNPVGTLFSRES 321
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHIISNEL AGSTHG +EFVSMAEI+ E+LD+NRVHI+YGLS DLS
Sbjct: 322 LYNLLDFAREKNIHIISNELF-AGSTHGSEEFVSMAEIVELEDLDQNRVHIVYGLSKDLS 380
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G + GVIY+FN+NV AAK+LTRFSS+S P+Q LLISMLSD KFV++ ++ + ERL
Sbjct: 381 LPGFRAGVIYSFNKNVLAAAKRLTRFSSISAPSQQLLISMLSDTKFVQKFIDANRERLRG 440
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
M+++F GLK+LGIEC KS+GGF CWADMSGLIRSY+EKGELELWD+LLN+AK+N GS
Sbjct: 441 MHLKFLTGLKQLGIECTKSNGGFNCWADMSGLIRSYNEKGELELWDRLLNVAKVNVTPGS 500
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCR 101
SC+C+EPGWF FC ++L+E+DI V+ RI ++S+TC+
Sbjct: 501 SCHCIEPGWFRFCLSTLTEKDIPVVMDRIRKISETCK 537
>ref|XP_006363709.1| PREDICTED: probable aminotransferase ACS10-like [Solanum tuberosum]
Length = 550
Score = 407 bits (1045), Expect = e-111
Identities = 198/278 (71%), Positives = 240/278 (86%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNF+LSI ALDRAFNQAKKRGL+VRGIIISNPSNPVG ++ RET
Sbjct: 272 KWRTGVEIIPVPCRSADNFNLSIDALDRAFNQAKKRGLKVRGIIISNPSNPVGNIFSRET 331
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFATEKNIH+ISNE+L AGST+G +EFVS+AEI+++E+ DR+RVHI+YGLS DLS
Sbjct: 332 LYNLLDFATEKNIHVISNEIL-AGSTYGREEFVSIAEIIDSEDFDRSRVHIVYGLSKDLS 390
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+ NENV AAKKLTRFSS+S PTQHL+I MLSD KFV+Q ++ + ERL +
Sbjct: 391 LPGFRVGVIYSCNENVLAAAKKLTRFSSISAPTQHLIIQMLSDAKFVQQFIKKNRERLRK 450
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
M F +GLK+LGIEC KS GGFYCWADMS LIRSY+EKGE+ELWD LLN+AKIN+ GS
Sbjct: 451 MSSLFVSGLKQLGIECTKSSGGFYCWADMSRLIRSYNEKGEIELWDNLLNVAKINATPGS 510
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+CVEPGWF CF++LSE+DI +V+ RI +V + +S
Sbjct: 511 SCHCVEPGWFRLCFSTLSEKDISTVMQRIQKVLELRKS 548
>ref|XP_004298716.1| PREDICTED: probable aminotransferase ACS10-like [Fragaria vesca
subsp. vesca]
Length = 535
Score = 404 bits (1039), Expect = e-110
Identities = 192/278 (69%), Positives = 238/278 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS D F++SI+ALDRAFNQAKKRG++VRGIIISNPSNP G L R++
Sbjct: 257 KWRTGVEIIPVPCRSADKFNMSISALDRAFNQAKKRGVKVRGIIISNPSNPGGGLLTRDS 316
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DF EKNIHIISNEL AGST+G++EFVSMAEI++ E+LD+NRVHI+YGLS DLS
Sbjct: 317 LYNLLDFVREKNIHIISNELF-AGSTYGNEEFVSMAEIVDLEDLDQNRVHIVYGLSKDLS 375
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++G IY+FN+NV AAK+LTRFSS+S P+Q LLISMLSD KF+ + +E + ERL
Sbjct: 376 LPGFRVGAIYSFNQNVLTAAKRLTRFSSISAPSQRLLISMLSDTKFIHKFIEINRERLRG 435
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY++F GLK+LGIEC KS+GGFYCWAD+SGLIRSYSEKGELELWD+LLN+ K+N GS
Sbjct: 436 MYLKFVTGLKQLGIECTKSNGGFYCWADLSGLIRSYSEKGELELWDRLLNVGKVNVTPGS 495
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWF FCF +L+E+DI V+ RI +S++C+S
Sbjct: 496 SCHCIEPGWFRFCFTTLTEKDIPVVMARIRSISESCKS 533
>ref|XP_006440457.1| hypothetical protein CICLE_v10019580mg [Citrus clementina]
gi|557542719|gb|ESR53697.1| hypothetical protein
CICLE_v10019580mg [Citrus clementina]
Length = 547
Score = 404 bits (1038), Expect = e-110
Identities = 197/280 (70%), Positives = 237/280 (84%), Gaps = 2/280 (0%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNFSLSITALDRAFN A+KRGL+VRGIIISNPSNPVG + +RET
Sbjct: 267 KWRTGVEIIPVPCRSADNFSLSITALDRAFNHARKRGLKVRGIIISNPSNPVGNVINRET 326
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHG--DDEFVSMAEILNTENLDRNRVHIIYGLSND 578
LYSL+DFA EKNIHI+SNE+L AGST G +EFVS+AEI+++E LDR+RVH +Y LS D
Sbjct: 327 LYSLLDFAREKNIHIVSNEIL-AGSTFGIGTEEFVSLAEIVDSEYLDRDRVHTVYNLSQD 385
Query: 577 LSLTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL 398
LSL G +I VIY++N +V AAKKL RFSSVS P+QHLL+SMLSD KFV++ + + ERL
Sbjct: 386 LSLPGFRISVIYSYNNSVLAAAKKLARFSSVSAPSQHLLVSMLSDTKFVQKFININRERL 445
Query: 397 *RMYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLS 218
R+Y++F GL++LGIEC KS+GGFYCWADMSGLI SYSEKGELELWDKLLN+AK+N
Sbjct: 446 QRLYVKFVAGLRQLGIECAKSNGGFYCWADMSGLISSYSEKGELELWDKLLNVAKVNVTP 505
Query: 217 GSSCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
GSSC+C+EPGWF F F L+E+DIH V+ RI +S+TC+S
Sbjct: 506 GSSCHCIEPGWFSFSFTLLTEKDIHVVMERIRRISQTCKS 545
>gb|ABY55589.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa hybrid cultivar]
Length = 488
Score = 404 bits (1038), Expect = e-110
Identities = 195/278 (70%), Positives = 234/278 (84%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPC S D F+LSITALDRAFNQAKKRG++VRGIIISNPSNP G L RE
Sbjct: 210 KWRTGVEIIPVPCCSADKFNLSITALDRAFNQAKKRGVKVRGIIISNPSNPGGGLLTRER 269
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHIISNEL AGST+G +EFVS+AEI++ E+LD+NRVHI+YGLS DLS
Sbjct: 270 LYNLLDFAREKNIHIISNELF-AGSTYGSEEFVSLAEIVDLEDLDQNRVHIVYGLSKDLS 328
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++G IY+FN+NV AAKKLTRFSS+S P+Q LLISMLSD KF+ + +E + ERL
Sbjct: 329 LPGFRVGAIYSFNKNVLSAAKKLTRFSSISAPSQRLLISMLSDTKFMHKFIEMNRERLRG 388
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ F GLK+LGIEC KS GGFYCWAD+SGLIRSYSEKGELELWD+LLN+ K+N GS
Sbjct: 389 MYLRFVTGLKQLGIECTKSSGGFYCWADLSGLIRSYSEKGELELWDRLLNVGKLNVTPGS 448
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWF FCF +L+E+DI V+ RI +++TC+S
Sbjct: 449 SCHCIEPGWFRFCFTTLTEKDIPVVMERIRNIAETCKS 486
>ref|XP_002304448.1| 1-aminocyclopropane-1-carboxylate synthase family protein [Populus
trichocarpa] gi|222841880|gb|EEE79427.1|
1-aminocyclopropane-1-carboxylate synthase family protein
[Populus trichocarpa]
Length = 551
Score = 404 bits (1037), Expect = e-110
Identities = 197/278 (70%), Positives = 233/278 (83%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNFSLSITALDR FNQAKKRGL+VRGI+ISNPSNPVG L +RET
Sbjct: 273 KWRTGVEIIPVPCRSADNFSLSITALDRGFNQAKKRGLKVRGIMISNPSNPVGNLLNRET 332
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LYSL+DFA EKNIHI+SNE+ AGSTHG +EF SMAE+++++ LDR+RVHI+YGLS DLS
Sbjct: 333 LYSLLDFAREKNIHIVSNEIF-AGSTHGSEEFASMAELIDSDYLDRDRVHIVYGLSKDLS 391
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+FNENV AAKKLTRFSSVS PTQHLL+S+LSD FV+++++ + ERL +
Sbjct: 392 LPGFRVGVIYSFNENVLAAAKKLTRFSSVSAPTQHLLVSLLSDTDFVQKLIKNNRERLQK 451
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
Y F GLK+LGIEC KS GGF CWADMS LI SYSEKGELELWDKLLN AK+N GS
Sbjct: 452 TYATFVAGLKQLGIECAKSKGGFSCWADMSELINSYSEKGELELWDKLLNTAKLNVTPGS 511
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
C C+EPGWF FCFA+L+ERDI V+ RI ++ C++
Sbjct: 512 CCRCIEPGWFQFCFANLTERDIPVVMDRILKIVNVCKA 549
>ref|XP_002281504.2| PREDICTED: probable aminotransferase ACS10-like, partial [Vitis
vinifera]
Length = 310
Score = 403 bits (1036), Expect = e-110
Identities = 197/278 (70%), Positives = 238/278 (85%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNF+LSI+ALD AF+Q KKRGL+VRGI+ISNPSNPVG L +RET
Sbjct: 32 KWRTGVEIIPVPCRSADNFNLSISALDLAFDQGKKRGLKVRGIVISNPSNPVGNLLNRET 91
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
+YSL+DFA EKNIHIISNE+ AGSTHG +EFVSMAEI+++E+LDR+RVHI+YGLS DL
Sbjct: 92 IYSLVDFAREKNIHIISNEIF-AGSTHGSEEFVSMAEIIDSEDLDRDRVHIVYGLSKDLC 150
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L K+GVIY+ NENV AAKKL+RFSS+S PTQ L+ISMLSD +F+++ ++ + ERL R
Sbjct: 151 LPRFKVGVIYSSNENVLAAAKKLSRFSSISAPTQCLVISMLSDIRFIQKFIQTNRERLQR 210
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY +F GLK+LGIECM+S GGFYCWADM GLIRSYSEKGELELW+KLLN+AKIN GS
Sbjct: 211 MYTKFVAGLKQLGIECMRSSGGFYCWADMRGLIRSYSEKGELELWNKLLNIAKINVTPGS 270
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
SC+C+EPGWF CF +L+E+DI V+ RI +VS+TC S
Sbjct: 271 SCHCIEPGWFRCCFTTLTEKDIPVVMERIRKVSETCIS 308
>ref|XP_006477323.1| PREDICTED: probable aminotransferase ACS10-like [Citrus sinensis]
Length = 547
Score = 403 bits (1035), Expect = e-110
Identities = 197/280 (70%), Positives = 238/280 (85%), Gaps = 2/280 (0%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNFSLSITALDRAFNQA+KRGL+VRGIIISNPSNPVG + +RET
Sbjct: 267 KWRTGVEIIPVPCRSADNFSLSITALDRAFNQARKRGLKVRGIIISNPSNPVGNVINRET 326
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHG--DDEFVSMAEILNTENLDRNRVHIIYGLSND 578
LYSL+DFA EKNIHI+SNE+L AGST G +EFVS+AEI+++E LDR+RVH +Y LS D
Sbjct: 327 LYSLLDFAREKNIHIVSNEIL-AGSTFGIGTEEFVSLAEIVDSEYLDRDRVHTVYDLSQD 385
Query: 577 LSLTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL 398
LSL G +I VIY++N +V AAKKL RFSSVS P+Q+LL+SMLSD KFV++ + + ERL
Sbjct: 386 LSLPGFRISVIYSYNNSVLAAAKKLARFSSVSAPSQNLLVSMLSDTKFVQKFININRERL 445
Query: 397 *RMYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLS 218
R+Y++F GL++LGIEC KS+GGFYCWADMSGLI SYSEKGELELWDKLLN+AK+N
Sbjct: 446 RRLYVKFVAGLRQLGIECAKSNGGFYCWADMSGLISSYSEKGELELWDKLLNVAKVNVTP 505
Query: 217 GSSCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
GSSC+C+EPGWF F F L+E+DIH V+ RI +S+TC+S
Sbjct: 506 GSSCHCIEPGWFSFSFTLLTEKDIHVVMERIRRISQTCKS 545
>emb|CBI23558.3| unnamed protein product [Vitis vinifera]
Length = 352
Score = 396 bits (1017), Expect = e-108
Identities = 198/305 (64%), Positives = 242/305 (79%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS DNF+LSI+ALD AF+Q KKRGL+VRGI+ISNPSNPVG L +RET
Sbjct: 35 KWRTGVEIIPVPCRSADNFNLSISALDLAFDQGKKRGLKVRGIVISNPSNPVGNLLNRET 94
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
+YSL+DFA EKNIHIISNE+ AGSTHG +EFVSMAEI+++E+LDR+RVHI+YGLS DL
Sbjct: 95 IYSLVDFAREKNIHIISNEIF-AGSTHGSEEFVSMAEIIDSEDLDRDRVHIVYGLSKDLC 153
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L K+GVIY+ NENV AAKKL+RFSS+S PTQ L+ISMLSD +F+++ ++ + ERL R
Sbjct: 154 LPRFKVGVIYSSNENVLAAAKKLSRFSSISAPTQCLVISMLSDIRFIQKFIQTNRERLQR 213
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY +F GLK+LGIECM+S GGFYCWADM GLIRSYSEKGELELW+KLLN+AKIN GS
Sbjct: 214 MYTKFVAGLKQLGIECMRSSGGFYCWADMRGLIRSYSEKGELELWNKLLNIAKINVTPGS 273
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRSF*LECY*FIVDNLYRFSCSFFV 32
SC+C+EPGWF CF +L+E+DI V+ RI + + S CY + + F
Sbjct: 274 SCHCIEPGWFRCCFTTLTEKDIPVVMERIRK-NNISFSLGFGCYSDTIKSSSHVPLQLFC 332
Query: 31 FIPEF 17
F P +
Sbjct: 333 FSPSY 337
>gb|ABY55588.1| 1-aminocyclopropane-1-carboxylate synthase [Rosa hybrid cultivar]
Length = 490
Score = 395 bits (1015), Expect = e-107
Identities = 193/290 (66%), Positives = 236/290 (81%), Gaps = 12/290 (4%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRS D F+LSITALDRAFNQAKKRG++ +GIIISNPSNP G L+ RE+
Sbjct: 200 KWRTGVEIIPVPCRSADKFNLSITALDRAFNQAKKRGVKGKGIIISNPSNPGGSLFTRES 259
Query: 751 LYSLMDFATEKNIHIIS------------NELLLAGSTHGDDEFVSMAEILNTENLDRNR 608
+Y+L+ FA EKNIHIIS NEL AGST+G +EFVSMAEI++ E+LD+NR
Sbjct: 260 IYNLLYFAREKNIHIISLCPKEEHSYSLSNELF-AGSTYGSEEFVSMAEIVDLEDLDQNR 318
Query: 607 VHIIYGLSNDLSLTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVR 428
VHI+YG+S DLSL G ++G IY+FN+NV AAKKLTRFSS+S P+Q LLISMLSD KF+
Sbjct: 319 VHIVYGISKDLSLPGFRVGAIYSFNKNVLTAAKKLTRFSSISAPSQRLLISMLSDTKFMH 378
Query: 427 QIVEFSTERL*RMYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKL 248
+ +E + ERL MY+ F GLK+LGIEC KS+GGFYCWAD+SGLIRSYSEKGELELWD+L
Sbjct: 379 KFIEINRERLRGMYLRFVTGLKQLGIECTKSNGGFYCWADLSGLIRSYSEKGELELWDRL 438
Query: 247 LNMAKINSLSGSSCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
LN+ K+N GSSC+C+EPGWF FCF +L+E+DI VI RI +++TC+S
Sbjct: 439 LNVGKLNVTPGSSCHCIEPGWFRFCFTTLTEKDIPVVIERIRNIAETCKS 488
>ref|XP_002268932.1| PREDICTED: probable aminotransferase ACS12-like [Vitis vinifera]
Length = 503
Score = 384 bits (986), Expect = e-104
Identities = 186/275 (67%), Positives = 228/275 (82%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVE+IPV CRS+DNF+LS+TALD+AFNQA+KRG RVRGI+ISNPSNP+G + ++ET
Sbjct: 230 KWRTGVELIPVHCRSSDNFALSVTALDQAFNQARKRGQRVRGILISNPSNPIGNMLNKET 289
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LYSL+DFA EKNIHIIS+E+ AGST+G +EFVSMAEIL +E+ D++RVHIIYGLS DLS
Sbjct: 290 LYSLLDFAREKNIHIISDEIF-AGSTYGSEEFVSMAEILESEDFDKDRVHIIYGLSKDLS 348
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+FN +V AAKKL RFSSVS PTQ +LISMLSD +F+++ +E + ER+ +
Sbjct: 349 LPGFRVGVIYSFNTSVLAAAKKLARFSSVSAPTQRILISMLSDTRFIQRYIETTRERIQK 408
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ FA GLK+LGIEC KS G YCWADMSGLIR Y+EKGELELWDKLL + KIN GS
Sbjct: 409 MYVAFAAGLKQLGIECTKSSAGLYCWADMSGLIRPYNEKGELELWDKLLKIGKINVTPGS 468
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKT 107
+C+C+EPGWF CF L+E DI VI RI V +T
Sbjct: 469 ACHCIEPGWFRCCFTMLAEEDIPVVIKRIQRVVET 503
>gb|EXC17820.1| putative aminotransferase ACS10 [Morus notabilis]
Length = 557
Score = 384 bits (985), Expect = e-104
Identities = 189/278 (67%), Positives = 226/278 (81%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEIIPVPCRSTDNFSLSITALD AFNQAKKRGL+VRGII+SNPSNPVG L RET
Sbjct: 279 KWRTGVEIIPVPCRSTDNFSLSITALDLAFNQAKKRGLKVRGIILSNPSNPVGNLLSRET 338
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
L SL+DFA EKNIHIISNE+ AGST G +EF+SMAEI+++E+LDR+R+HI+Y LS DLS
Sbjct: 339 LSSLLDFAREKNIHIISNEIF-AGSTRGSEEFISMAEIIDSEDLDRDRIHIVYSLSKDLS 397
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
+ G ++ V+Y+FNENV AAKKL R SS S P+Q LLISMLSD KFV +E + ERL R
Sbjct: 398 IPGFRVSVLYSFNENVVAAAKKLARVSSTSAPSQRLLISMLSDAKFVETFIETNGERLRR 457
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY +F GL++LGIEC KS+GGFYCWADMS I SYSEKGELELW++LL++AK+N GS
Sbjct: 458 MYRKFVAGLEQLGIECTKSNGGFYCWADMSRFIFSYSEKGELELWERLLSVAKVNVTPGS 517
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEVSKTCRS 98
+C+EPGWF CF +L+E DI V+ RI +S C+S
Sbjct: 518 CFHCIEPGWFRLCFTNLAESDIPVVMERIRRISVACKS 555
>emb|CAN66821.1| hypothetical protein VITISV_003497 [Vitis vinifera]
Length = 540
Score = 382 bits (982), Expect = e-104
Identities = 185/272 (68%), Positives = 226/272 (83%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVE+IPV CRS+DNF+LS+TALD+AFNQA+KRG RVRGI+ISNPSNP+G + ++ET
Sbjct: 265 KWRTGVELIPVHCRSSDNFALSVTALDQAFNQARKRGQRVRGILISNPSNPIGNMLNKET 324
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LYSL+DFA EKNIHIIS+E+ AGST+G +EFVSMAEIL +E+ D++RVHIIYGLS DLS
Sbjct: 325 LYSLLDFAREKNIHIISDEIF-AGSTYGSEEFVSMAEILGSEDFDKDRVHIIYGLSKDLS 383
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G ++GVIY+FN +V AAKKL RFSSVS PTQ +LISMLSD +F+++ +E + ER+ +
Sbjct: 384 LPGFRVGVIYSFNTSVLAAAKKLARFSSVSAPTQRILISMLSDTRFIQRYIETTRERIQK 443
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
MY+ FA GLK+LGIEC KS G YCWADMSGLIR Y+EKGELELWDKLL + KIN GS
Sbjct: 444 MYVAFAAGLKQLGIECTKSSAGLYCWADMSGLIRPYNEKGELELWDKLLKIGKINVTPGS 503
Query: 211 SCYCVEPGWFGFCFASLSERDIHSVIHRIGEV 116
+C+C+EPGWF CF L+E DI VI RI V
Sbjct: 504 ACHCIEPGWFRCCFTMLAEEDIPVVIKRIQRV 535
>gb|AAG16977.1|AF184076_1 ACC synthase [Prunus armeniaca]
Length = 269
Score = 377 bits (969), Expect = e-102
Identities = 182/250 (72%), Positives = 217/250 (86%)
Frame = -1
Query: 931 KWRTGVEIIPVPCRSTDNFSLSITALDRAFNQAKKRGLRVRGIIISNPSNPVGILYDRET 752
KWRTGVEII VPCRS D F+LS+TALDRAFNQAKKRGL+VRGIIISNPSNPVG L+ RE+
Sbjct: 18 KWRTGVEIITVPCRSADKFNLSVTALDRAFNQAKKRGLKVRGIIISNPSNPVGTLFSRES 77
Query: 751 LYSLMDFATEKNIHIISNELLLAGSTHGDDEFVSMAEILNTENLDRNRVHIIYGLSNDLS 572
LY+L+DFA EKNIHIISNEL AGSTHG +EFVSMA+I+ E+LD+NRVHI+YGLS DLS
Sbjct: 78 LYNLLDFAREKNIHIISNELF-AGSTHGSEEFVSMADIVELEDLDQNRVHIVYGLSKDLS 136
Query: 571 LTGSKIGVIYTFNENVAFAAKKLTRFSSVSVPTQHLLISMLSDKKFVRQIVEFSTERL*R 392
L G + GVIY+FN+NV AAK+LTRFSS+S P+Q LL SMLSD KFV++ ++ + ERL
Sbjct: 137 LPGFRAGVIYSFNKNVLAAAKRLTRFSSISTPSQQLLSSMLSDTKFVQKFIDANRERLRG 196
Query: 391 MYIEFANGLKKLGIECMKSDGGFYCWADMSGLIRSYSEKGELELWDKLLNMAKINSLSGS 212
M+++F GLK+LGIEC KS+GGF CWADMSGLIRSY+EKGELELWD+LLN+AK+N GS
Sbjct: 197 MHLKFLTGLKQLGIECTKSNGGFSCWADMSGLIRSYNEKGELELWDRLLNVAKVNVTPGS 256
Query: 211 SCYCVEPGWF 182
SC+C+EPGWF
Sbjct: 257 SCHCIEPGWF 266