BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia22_contig00013930
         (1350 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|...   597   e-168
gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]         593   e-167
gb|AAY84833.1| isoamylase [Ipomoea batatas]                           587   e-165
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like ...   585   e-164
gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]               576   e-162
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...   575   e-161
ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, par...   575   e-161
ref|XP_002308090.2| isoamylase family protein [Populus trichocar...   572   e-160
ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Popu...   572   e-160
ref|XP_002324659.2| isoamylase family protein [Populus trichocar...   566   e-159
ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|2...   562   e-157
ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like ...   560   e-157
ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like ...   560   e-157
ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like ...   559   e-156
gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [...   557   e-156
ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like ...   553   e-155
emb|CBI40669.3| unnamed protein product [Vitis vinifera]              553   e-155
dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Pha...   552   e-154
ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like ...   550   e-154
gb|EXC12839.1| Isoamylase 1 [Morus notabilis]                         544   e-152

>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|27728145|gb|AAN15317.1|
            isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  597 bits (1540), Expect = e-168
 Identities = 288/406 (70%), Positives = 324/406 (79%)
 Frame = +2

Query: 131  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNNASKRGD 310
            ++LLH   I +Y  PK +   +L  R++       S   ++  + VN  V          
Sbjct: 1    MELLHCPSISTY-KPKLSFHNHLFSRRSSNGVDFESIWRKSRSSVVNAAVDSGRGGVVKT 59

Query: 311  TETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLRE 490
              TAVVVEKP     RFEV SG P PFGAT  DGGV            TLCLI+LSDL E
Sbjct: 60   AATAVVVEKPTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPE 119

Query: 491  KRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYA 670
            KRVTEQI L+PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++DPYA
Sbjct: 120  KRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYA 179

Query: 671  KAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTR 850
            KA+VSRGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK PQ DLVIYEMHVRGFT 
Sbjct: 180  KAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTN 239

Query: 851  HESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGY 1030
            HESS +KYPGTYLG+VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NFWGY
Sbjct: 240  HESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGY 299

Query: 1031 STINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPI 1210
            ST+NFFSPM RYSSAG+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNENGPI
Sbjct: 300  STVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPI 359

Query: 1211 LSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            LSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCLR
Sbjct: 360  LSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLR 405


>gb|EPS64233.1| isoamylase isoform 1, partial [Genlisea aurea]
          Length = 774

 Score =  593 bits (1528), Expect = e-167
 Identities = 280/386 (72%), Positives = 316/386 (81%), Gaps = 4/386 (1%)
 Frame = +2

Query: 203  CRKNKLNFGIPSKTFENTGTKVNPMVILNNASKR--GDTETAVVVEKPPAM--PRRFEVF 370
            CR+N+   G   +     G    P V+++  ++R  G      ++EK      P RFEV 
Sbjct: 4    CRRNEFTVGNRRRFLVLCGEGAKPSVVVDEVNERRSGKRRPPALLEKTKIREEPSRFEVS 63

Query: 371  SGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVTEQIALNPLTNKTGDVW 550
            SG P PFGATPRDGGV             LCLISL DL E +VTE+I LNPLTNKTGDVW
Sbjct: 64   SGQPMPFGATPRDGGVNFAIFSSNASLAALCLISLGDLSEGKVTEEITLNPLTNKTGDVW 123

Query: 551  HVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWP 730
            H+FLKGDFE+MAYGY+FDGSFSPEDG YFDSSK+LIDPYAKA+  RGEYGAL PD +CWP
Sbjct: 124  HIFLKGDFENMAYGYRFDGSFSPEDGLYFDSSKILIDPYAKAIAGRGEYGALAPDGNCWP 183

Query: 731  QMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLD 910
            QMA +VPST DEFDWEGDLPL+LPQ +L+IYEMHVRGFTRHESS +++PGTY G+VEKL+
Sbjct: 184  QMASMVPSTVDEFDWEGDLPLRLPQRELIIYEMHVRGFTRHESSMTEFPGTYSGVVEKLE 243

Query: 911  YLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNY 1090
            YLKK+GVNCIELMPC EFNELE+YSYN VLGDYKMNFWGYS INFFSPMARYSSAG+ N+
Sbjct: 244  YLKKIGVNCIELMPCQEFNELEYYSYNRVLGDYKMNFWGYSPINFFSPMARYSSAGVQNH 303

Query: 1091 GLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEY 1270
            GLGAVNEFK+LV+EAHK+GIEVIMDVVFNHTAEGNENGP LSFRG+DNSVFYMLAP GEY
Sbjct: 304  GLGAVNEFKHLVKEAHKKGIEVIMDVVFNHTAEGNENGPTLSFRGVDNSVFYMLAPMGEY 363

Query: 1271 YNYSGCGNTFNCNHPIVRQFILDCLR 1348
            YNYSGCGNTFNCNHP VRQFI+DCLR
Sbjct: 364  YNYSGCGNTFNCNHPFVRQFIVDCLR 389


>gb|AAY84833.1| isoamylase [Ipomoea batatas]
          Length = 785

 Score =  587 bits (1512), Expect = e-165
 Identities = 280/410 (68%), Positives = 327/410 (79%), Gaps = 4/410 (0%)
 Frame = +2

Query: 131  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNNAS--KR 304
            ++LLH   I  Y P   +    +R  K  L+FG P   F     K  P  +  NA+    
Sbjct: 1    MELLHSPSISQYSPKLHSRSSNIR--KTGLSFGFPHNYFAEFEKKARPSALAVNAAIDGG 58

Query: 305  GDTETAVVVEKPP--AMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLS 478
            G+++TAVVVEKP    + RRFEV SGHP PFGAT RDGG+             LCLISL+
Sbjct: 59   GESDTAVVVEKPLPYGLLRRFEVLSGHPAPFGATARDGGINFAVFSSNATSAALCLISLA 118

Query: 479  DLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLI 658
            DL EK+VTEQI L+P  NKTGDVWHVFL+GDF++M YGY FDG F+PE+G YFDS+++L+
Sbjct: 119  DLPEKKVTEQIPLDPSINKTGDVWHVFLQGDFDNMLYGYSFDGKFAPEEGHYFDSARILL 178

Query: 659  DPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVR 838
            DPYAKA+VSR E+GALGP+KDCWP MAC++P T D+FDWEGDLPLK PQ DLVIYEMHVR
Sbjct: 179  DPYAKAIVSRAEFGALGPEKDCWPPMACMLP-TADKFDWEGDLPLKFPQRDLVIYEMHVR 237

Query: 839  GFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMN 1018
            GFT HESS +++PGTY G+VEKLD+LK LGVNCIELMPCHEFNELE+YSYN VLGDYK+N
Sbjct: 238  GFTNHESSGTEFPGTYRGVVEKLDHLKVLGVNCIELMPCHEFNELEYYSYNPVLGDYKVN 297

Query: 1019 FWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNE 1198
            FWGYST+NFFSPM RYSSAG+   GLGA++EFKYLVREAH+RGIEVIMDVVFNHTAEGNE
Sbjct: 298  FWGYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGNE 357

Query: 1199 NGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            NGP+ SFRG+DNSVFYMLAPKGE+YNYSGCGNTFNCNHP+ RQFIL+ LR
Sbjct: 358  NGPMFSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVARQFILEFLR 407


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like [Solanum lycopersicum]
          Length = 787

 Score =  585 bits (1507), Expect = e-164
 Identities = 288/409 (70%), Positives = 324/409 (79%), Gaps = 3/409 (0%)
 Frame = +2

Query: 131  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNNASKRGD 310
            ++LLH   I +Y  PK   + +L  R+N          FE    K     ++N A  R  
Sbjct: 1    MELLHSPSISTY-KPKLNYQNHLFSRRNS-----KPVDFETIRRKS----VVNAAVHRLA 50

Query: 311  TETA---VVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSD 481
             +TA   VVVEK      RFEV SG P PFGAT  DGGV            TLCLI+LSD
Sbjct: 51   AKTAASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSD 110

Query: 482  LREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLID 661
            L EKRVTEQI L+PL NKTGDVWHVFLKGDFE+M YGYKFDG F PE+G YFDSS++++D
Sbjct: 111  LPEKRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLD 170

Query: 662  PYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRG 841
            PYAKA+VSRGEYG LGP+ DCWP MA +VPS +D+FDWEGDLPLK  Q +LVIYEMHVRG
Sbjct: 171  PYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHVRG 230

Query: 842  FTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNF 1021
            FT HESS +KYPGTYLG+VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK NF
Sbjct: 231  FTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNF 290

Query: 1022 WGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNEN 1201
            WGYST+NFFSPM RYSSAG+ N GLGA+NEFKYLV+EAHKRGIEVIMDVVFNHTAEGNEN
Sbjct: 291  WGYSTVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEN 350

Query: 1202 GPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            GPILSFRG+DNSVFY LAPKGE+YNYSGCGNTFNCN+PIVRQFI+DCLR
Sbjct: 351  GPILSFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLR 399


>gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score =  576 bits (1484), Expect = e-162
 Identities = 276/398 (69%), Positives = 317/398 (79%), Gaps = 6/398 (1%)
 Frame = +2

Query: 173  PKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVIL-----NNASKRGDTET-AVVVE 334
            PK  P    R    K N    S     T T    MV+         ++RG  E    VVE
Sbjct: 14   PKLIPATLRRQTPPKPNLRTVSNNHATTST--TRMVVKARGGGGGGARRGAAEADTAVVE 71

Query: 335  KPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVTEQIA 514
            KP    +RF++  GHP PFGAT  DGGV            TLCLI+ SDL++ RVTEQI+
Sbjct: 72   KPKI--KRFQLSEGHPGPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQNRVTEQIS 129

Query: 515  LNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVVSRGE 694
            L+PLTNKTGDVWHVFL+GDF+DM YGY FDG FSPE G Y+DSSK+L+DPYAKAV+SRGE
Sbjct: 130  LDPLTNKTGDVWHVFLRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGE 189

Query: 695  YGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTRHESSNSKY 874
            +GALGP+ +CWPQMAC+VP++ DEFDWEGDLPL+  Q DL+IYEMHVRGFTRHESS +++
Sbjct: 190  FGALGPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEF 249

Query: 875  PGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTINFFSP 1054
            PGTY G+VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNS+LGDYK+NFWGYST+NFFSP
Sbjct: 250  PGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSP 309

Query: 1055 MARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDN 1234
            M RYSS+G+ N G  AVNEFKYLV+EAHKRGIEV MDVVFNHTAEGNENGPILSFRG+DN
Sbjct: 310  MIRYSSSGMHNCGRDAVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDN 369

Query: 1235 SVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            SV+YMLAPKGEYYNYSGCGNTFNCNHP+VRQFILDCLR
Sbjct: 370  SVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLR 407


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score =  575 bits (1483), Expect = e-161
 Identities = 272/393 (69%), Positives = 321/393 (81%), Gaps = 2/393 (0%)
 Frame = +2

Query: 176  KFTPKKYLRC--RKNKLNFGIPSKTFENTGTKVNPMVILNNASKRGDTETAVVVEKPPAM 349
            K  P    RC    NK +    SK FEN         ++  ASK  + ETAV+ EKPP+ 
Sbjct: 33   KKPPTTTFRCCNHPNKTSHFANSKAFENVTKN-----LVIRASKSAELETAVI-EKPPS- 85

Query: 350  PRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVTEQIALNPLT 529
             +RF+V  G+PTPFGAT RDGGV            +LCLI+LSDL+E +VTE+IAL+   
Sbjct: 86   -QRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSASLCLITLSDLQENKVTEEIALDSFA 144

Query: 530  NKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVVSRGEYGALG 709
            NKTGDVWHVFLKGDF+DM YGYKFDG FSP++G YFD +K+++DPYAKAV+SR ++G LG
Sbjct: 145  NKTGDVWHVFLKGDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLG 204

Query: 710  PDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTRHESSNSKYPGTYL 889
            PD++CWPQMACLVP+  DEFDWEGDLPLK PQ DL+IYE+HVRGFTRHESS +++PGTYL
Sbjct: 205  PDENCWPQMACLVPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYL 264

Query: 890  GMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTINFFSPMARYS 1069
            G+VEKLD+LK LG+NC+ELMPCHEFNELE++SYNSVLGDYK+NFWGYSTIN+FSPM  YS
Sbjct: 265  GVVEKLDHLKDLGINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTINYFSPMISYS 324

Query: 1070 SAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDNSVFYM 1249
            SAGI N G  A+NEFK LVREAHKRGIEV+MDVVFNHTAEGN+ GPILSFRG+DNSV+YM
Sbjct: 325  SAGIRNCGHDAINEFKLLVREAHKRGIEVVMDVVFNHTAEGNDKGPILSFRGVDNSVYYM 384

Query: 1250 LAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            LAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLR
Sbjct: 385  LAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLR 417


>ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, partial [Citrus clementina]
            gi|557556429|gb|ESR66443.1| hypothetical protein
            CICLE_v100074892mg, partial [Citrus clementina]
          Length = 624

 Score =  575 bits (1483), Expect = e-161
 Identities = 272/393 (69%), Positives = 321/393 (81%), Gaps = 2/393 (0%)
 Frame = +2

Query: 176  KFTPKKYLRC--RKNKLNFGIPSKTFENTGTKVNPMVILNNASKRGDTETAVVVEKPPAM 349
            K  P    RC    NK +    SK FEN         ++  ASK  + ETAV+ EKPP+ 
Sbjct: 33   KKPPTTTFRCCNHPNKTSHFANSKAFENVTKN-----LVIRASKSAELETAVI-EKPPS- 85

Query: 350  PRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVTEQIALNPLT 529
             +RF+V  G+PTPFGAT RDGGV            +LCLI+LSDL+E +VTE+IAL+   
Sbjct: 86   -QRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSASLCLITLSDLQENKVTEEIALDSFA 144

Query: 530  NKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVVSRGEYGALG 709
            NKTGDVWHVFLKGDF+DM YGYKFDG FSP++G YFD +K+++DPYAKAV+SR ++G LG
Sbjct: 145  NKTGDVWHVFLKGDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLG 204

Query: 710  PDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTRHESSNSKYPGTYL 889
            PD++CWPQMACLVP+  DEFDWEGDLPLK PQ DL+IYE+HVRGFTRHESS +++PGTYL
Sbjct: 205  PDENCWPQMACLVPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYL 264

Query: 890  GMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTINFFSPMARYS 1069
            G+VEKLD+LK LG+NC+ELMPCHEFNELE++SYNSVLGDYK+NFWGYSTIN+FSPM  YS
Sbjct: 265  GVVEKLDHLKDLGINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTINYFSPMISYS 324

Query: 1070 SAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDNSVFYM 1249
            SAGI N G  A+NEFK LVREAHKRGIEV+MDVVFNHTAEGN+ GPILSFRG+DNSV+YM
Sbjct: 325  SAGIRNCGHDAINEFKLLVREAHKRGIEVVMDVVFNHTAEGNDKGPILSFRGVDNSVYYM 384

Query: 1250 LAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            LAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLR
Sbjct: 385  LAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLR 417


>ref|XP_002308090.2| isoamylase family protein [Populus trichocarpa]
            gi|550335674|gb|EEE91613.2| isoamylase family protein
            [Populus trichocarpa]
          Length = 876

 Score =  572 bits (1474), Expect = e-160
 Identities = 272/412 (66%), Positives = 321/412 (77%)
 Frame = +2

Query: 113  HIVEMELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNN 292
            H +++   + H+   P + PPK      L          I S  F N+      ++ +  
Sbjct: 59   HSLQLPKFISHFQNTPKFYPPKRVTTSNLET--------IGSNIFSNS----TALIPIKA 106

Query: 293  ASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLIS 472
            AS+  DT   VV E+ P + ++ +VF GHP PFGAT RDGGV            TLCLIS
Sbjct: 107  ASEGVDTAVVVVEEEEPKL-KKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLIS 165

Query: 473  LSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKV 652
            LSDL E RVTEQI L+PLTNKTGDVWHV LKGDF+DM YGYKFDG+FSPE G Y+D SK+
Sbjct: 166  LSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPSKI 225

Query: 653  LIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMH 832
            ++DPYAK+V+SRGE+G LG D + WPQMAC++P+  ++FDWEGD PLK PQ DL+IYEMH
Sbjct: 226  VLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYEMH 285

Query: 833  VRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYK 1012
            VRGFT+HESS +++PGTYLG+VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK
Sbjct: 286  VRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYK 345

Query: 1013 MNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEG 1192
            +NFWGYST+N+FSPM RYSSAG  N G  A+NEFK LVREAHKRGIEV MDVVFNHTAEG
Sbjct: 346  VNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHTAEG 405

Query: 1193 NENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            NE GPILSFRG+DNS++YMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCLR
Sbjct: 406  NEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLR 457


>ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Populus trichocarpa]
            gi|550335673|gb|ERP58934.1| hypothetical protein
            POPTR_0006s07010g [Populus trichocarpa]
          Length = 845

 Score =  572 bits (1474), Expect = e-160
 Identities = 272/412 (66%), Positives = 321/412 (77%)
 Frame = +2

Query: 113  HIVEMELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNN 292
            H +++   + H+   P + PPK      L          I S  F N+      ++ +  
Sbjct: 59   HSLQLPKFISHFQNTPKFYPPKRVTTSNLET--------IGSNIFSNS----TALIPIKA 106

Query: 293  ASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLIS 472
            AS+  DT   VV E+ P + ++ +VF GHP PFGAT RDGGV            TLCLIS
Sbjct: 107  ASEGVDTAVVVVEEEEPKL-KKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLIS 165

Query: 473  LSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKV 652
            LSDL E RVTEQI L+PLTNKTGDVWHV LKGDF+DM YGYKFDG+FSPE G Y+D SK+
Sbjct: 166  LSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPSKI 225

Query: 653  LIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMH 832
            ++DPYAK+V+SRGE+G LG D + WPQMAC++P+  ++FDWEGD PLK PQ DL+IYEMH
Sbjct: 226  VLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYEMH 285

Query: 833  VRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYK 1012
            VRGFT+HESS +++PGTYLG+VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNSVLGDYK
Sbjct: 286  VRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYK 345

Query: 1013 MNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEG 1192
            +NFWGYST+N+FSPM RYSSAG  N G  A+NEFK LVREAHKRGIEV MDVVFNHTAEG
Sbjct: 346  VNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHTAEG 405

Query: 1193 NENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            NE GPILSFRG+DNS++YMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCLR
Sbjct: 406  NEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLR 457


>ref|XP_002324659.2| isoamylase family protein [Populus trichocarpa]
            gi|550318648|gb|EEF03224.2| isoamylase family protein
            [Populus trichocarpa]
          Length = 794

 Score =  566 bits (1458), Expect = e-159
 Identities = 271/417 (64%), Positives = 320/417 (76%)
 Frame = +2

Query: 98   LEFTIHIVEMELQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPM 277
            L  T H V +   +  +   P +C  K           N  NFG  S  F N+   ++  
Sbjct: 4    LHSTSHSVHVLKFISQFRSTPKFCLLK------RNVTSNLENFG--SNLFSNSTASISIK 55

Query: 278  VILNNASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXT 457
                  + R   +TAVVVE+     R+F+VF GHP+PFGAT RDGGV            T
Sbjct: 56   ------AARDGVDTAVVVEEEGPKLRKFQVFEGHPSPFGATVRDGGVNFAIFSANAVSAT 109

Query: 458  LCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYF 637
            LCLISLSDL E RVTEQ  L+PLTNKTGDVWHV LKGDF+DM YGYKFDGSFSPE+G Y+
Sbjct: 110  LCLISLSDLPENRVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFDGSFSPEEGHYY 169

Query: 638  DSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLV 817
            D S++++DPYAKAV+SRG++G LG D +CWPQMAC++P+ +D+FDWEGD  LK PQ DL+
Sbjct: 170  DPSQIVLDPYAKAVISRGDFGVLGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDLI 229

Query: 818  IYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSV 997
            +YEMHVRGFT+HESS +++PGTYLG+VEKL +LK+LGVNCIELMPCHEFNELE+Y YNSV
Sbjct: 230  LYEMHVRGFTQHESSRTEFPGTYLGVVEKLGHLKELGVNCIELMPCHEFNELEYYDYNSV 289

Query: 998  LGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFN 1177
             GDYK+NFWGYST+N+FSPM RYSSAG  N G  A+NEFK LVREAHKRGIEV MDVVFN
Sbjct: 290  FGDYKVNFWGYSTVNYFSPMTRYSSAGTLNCGHDAINEFKLLVREAHKRGIEVFMDVVFN 349

Query: 1178 HTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            HTAEGNE GPILSF+G+DNSV+YMLAPKGE+YNYSGCGNTFNCNHP+VRQFILDCLR
Sbjct: 350  HTAEGNERGPILSFKGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLR 406


>ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|223530627|gb|EEF32503.1|
            isoamylase, putative [Ricinus communis]
          Length = 795

 Score =  562 bits (1449), Expect = e-157
 Identities = 261/357 (73%), Positives = 303/357 (84%), Gaps = 1/357 (0%)
 Frame = +2

Query: 281  ILNNASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTL 460
            ++  A++R + ETAVVV+KP    RR++V  GHP PFGAT  DGGV            +L
Sbjct: 55   VVTKAARRDEVETAVVVDKPKF--RRYQVSEGHPAPFGATIADGGVNFAIYSSDAVSASL 112

Query: 461  CLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFD 640
            CLISL DL + RV+E+IAL+P+ N+TGD+WHVFLKGDF+D  YGY+FDG  SP DG YFD
Sbjct: 113  CLISLDDLTQNRVSEEIALDPVRNRTGDIWHVFLKGDFKDTLYGYRFDGVLSPHDGHYFD 172

Query: 641  SSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTND-EFDWEGDLPLKLPQSDLV 817
            SS++++DPYAKAV+SRGE+G LGP  +CWPQMA ++PS +  EFDWEGDLPLK PQ DLV
Sbjct: 173  SSQIVLDPYAKAVISRGEFGVLGPGDNCWPQMAGMIPSASQAEFDWEGDLPLKYPQKDLV 232

Query: 818  IYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSV 997
            IYEMHVRGFTRHESS +++PGT+L  VEKLD+LK+LGVNCIELMPCHEFNELE+YSYNSV
Sbjct: 233  IYEMHVRGFTRHESSRTEFPGTFLATVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSV 292

Query: 998  LGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFN 1177
            LGDYKMNFWGYSTIN+FSPM RYSSAG  N G  A+NEFK+LVREAHKRGIEVIMDVVFN
Sbjct: 293  LGDYKMNFWGYSTINYFSPMTRYSSAGTRNCGRDAINEFKFLVREAHKRGIEVIMDVVFN 352

Query: 1178 HTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            HTAEGN+ GPILSFRG+DNSV+YMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCLR
Sbjct: 353  HTAEGNQKGPILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLR 409


>ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  560 bits (1442), Expect = e-157
 Identities = 261/361 (72%), Positives = 302/361 (83%), Gaps = 6/361 (1%)
 Frame = +2

Query: 284  LNNASKRGD---TETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXX 454
            LN A  RG    T  AV  E   +   RFEVF G PTPFGAT R+ G+            
Sbjct: 59   LNRARNRGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSA 118

Query: 455  TLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFY 634
            TLCLI LSDL+E RVT++I L+PLTNKTGDVWHVFLKGDF +M YGYKFDG FSP++G Y
Sbjct: 119  TLCLIHLSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCY 178

Query: 635  FDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTND---EFDWEGDLPLKLPQ 805
            FDSS++L+DPYAKAV+SRGE+GALGP+ +CWPQMA +VP+ N+   +FDWEGDLP++  Q
Sbjct: 179  FDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQ 238

Query: 806  SDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYS 985
             DL+IYEMHVRGFTRHESS S++PGTYLG+VEKLDYLK+LG+NCIELMPCHEFNELE++S
Sbjct: 239  KDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYFS 298

Query: 986  YNSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMD 1165
            YNSVLGDYKMNFWGYST+N+FSPM RYSSAGIGN G  A+NEFK L+REAHKRGIEVIMD
Sbjct: 299  YNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMD 358

Query: 1166 VVFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1345
            VVFNHTAEGNENGP +SFRG+DN+V+YMLAP GE+YNYSGCGNTFNCNHP VR FI+DCL
Sbjct: 359  VVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCL 418

Query: 1346 R 1348
            R
Sbjct: 419  R 419


>ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  560 bits (1442), Expect = e-157
 Identities = 261/361 (72%), Positives = 302/361 (83%), Gaps = 6/361 (1%)
 Frame = +2

Query: 284  LNNASKRGD---TETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXX 454
            LN A  RG    T  AV  E   +   RFEVF G PTPFGAT R+ G+            
Sbjct: 59   LNRARNRGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSA 118

Query: 455  TLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFY 634
            TLCLI LSDL+E RVT++I L+PLTNKTGDVWHVFLKGDF +M YGYKFDG FSP++G Y
Sbjct: 119  TLCLIHLSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCY 178

Query: 635  FDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTND---EFDWEGDLPLKLPQ 805
            FDSS++L+DPYAKAV+SRGE+GALGP+ +CWPQMA +VP+ N+   +FDWEGDLP++  Q
Sbjct: 179  FDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQ 238

Query: 806  SDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYS 985
             DL+IYEMHVRGFTRHESS S++PGTYLG+VEKLDYLK+LG+NCIELMPCHEFNELE++S
Sbjct: 239  KDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYFS 298

Query: 986  YNSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMD 1165
            YNSVLGDYKMNFWGYST+N+FSPM RYSSAGIGN G  A+NEFK L+REAHKRGIEVIMD
Sbjct: 299  YNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMD 358

Query: 1166 VVFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCL 1345
            VVFNHTAEGNENGP +SFRG+DN+V+YMLAP GE+YNYSGCGNTFNCNHP VR FI+DCL
Sbjct: 359  VVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCL 418

Query: 1346 R 1348
            R
Sbjct: 419  R 419


>ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like [Vitis vinifera]
          Length = 748

 Score =  559 bits (1440), Expect = e-156
 Identities = 261/348 (75%), Positives = 297/348 (85%)
 Frame = +2

Query: 305  GDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDL 484
            G     VVVEKP   P  F VF G P P GAT RDGGV            TLCLIS SDL
Sbjct: 14   GAEAETVVVEKPKLQP--FLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDL 71

Query: 485  REKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDP 664
             E RVTEQI+L+PLTNKTGDVWHVFLKG+FE++ YGYKFDG FSPE+G Y+DSS++L+DP
Sbjct: 72   EEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDP 131

Query: 665  YAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGF 844
            YAKAV+SRGE+G LGP+ +CWP MA ++PS++ EFDWEGDLPLK PQ DL+IYEMHVRGF
Sbjct: 132  YAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGF 191

Query: 845  TRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFW 1024
            TRHESS +K+PGTY G+VEKLD+LK+LGVNCIELMPCHEFNELE++SYNSVL DY++NFW
Sbjct: 192  TRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRVNFW 251

Query: 1025 GYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENG 1204
            GYST+N+FSPM RYSSAGI N G  A+NE K L+REAHKRGIEV+MDVVFNHTAEGNENG
Sbjct: 252  GYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGNENG 311

Query: 1205 PILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            PILSFRG+DNSV+YMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCLR
Sbjct: 312  PILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLR 359


>gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [Prunus persica]
          Length = 777

 Score =  557 bits (1436), Expect = e-156
 Identities = 256/358 (71%), Positives = 300/358 (83%)
 Frame = +2

Query: 275  MVILNNASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXX 454
            +V  +N     D     VV++P    RRF+V  GHP+PFGAT RDGGV            
Sbjct: 9    VVKASNGGAGTDVVETAVVDRPKL--RRFQVSQGHPSPFGATTRDGGVNFAVYSANAVSV 66

Query: 455  TLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFY 634
            TLCLI+LSDL E +VTEQ++L+PLTNKTG+VWHVFLKGDF+D  YGYKF+G FSPE+G Y
Sbjct: 67   TLCLITLSDLEEDKVTEQLSLDPLTNKTGNVWHVFLKGDFKDTLYGYKFEGKFSPEEGLY 126

Query: 635  FDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDL 814
            +DSSK+++DPYAKAV+ RGEYG L PD +CWPQMA  VPS +DEFDWEGDLPL  PQ DL
Sbjct: 127  YDSSKIVLDPYAKAVIRRGEYGKLSPDGNCWPQMAGTVPSFDDEFDWEGDLPLNYPQKDL 186

Query: 815  VIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNS 994
            +IYE+HVRGFTRHESS ++ PGTYLG+VEKLD+LK+LG+NCIELMPCHEFNELE++ YNS
Sbjct: 187  IIYEIHVRGFTRHESSMAELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEYFGYNS 246

Query: 995  VLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVF 1174
            VLGDYK+NFWGYST+N+FSPM RYSSAGI N G  A+NE K+L++EAHKRGIEVIMDVVF
Sbjct: 247  VLGDYKVNFWGYSTVNYFSPMIRYSSAGIRNCGRDAINEVKFLIKEAHKRGIEVIMDVVF 306

Query: 1175 NHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            NHTAEGNENGPILSFRG DNS++YMLA KGE+YNYSGCGNTFNCNHP+VRQFI+DCLR
Sbjct: 307  NHTAEGNENGPILSFRGADNSIYYMLAHKGEFYNYSGCGNTFNCNHPVVRQFIVDCLR 364


>ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like [Glycine max]
          Length = 798

 Score =  553 bits (1424), Expect = e-155
 Identities = 256/365 (70%), Positives = 300/365 (82%), Gaps = 3/365 (0%)
 Frame = +2

Query: 263  KVNPMVILNNASKRG---DTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXX 433
            K N ++    A+  G   +TETAVV  + P +   F+   G+P+PFGAT RDGGV     
Sbjct: 48   KTNSIIPSTRAAANGGGFETETAVV--EKPQLKTLFQASRGYPSPFGATVRDGGVNFAIS 105

Query: 434  XXXXXXXTLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSF 613
                   TLC  +LSD +  RVTE + L+PL N+TG VWHVFLKGDF DM YGYKFDG F
Sbjct: 106  SLNALSATLCFFTLSDFQNNRVTEYVPLDPLINRTGGVWHVFLKGDFRDMLYGYKFDGKF 165

Query: 614  SPEDGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPL 793
            SP +G YFDSS +L+DPYAKAV+SRGE+GALGPD +CWPQMA  VPS +DEFDWEGDLPL
Sbjct: 166  SPLEGHYFDSSHILLDPYAKAVISRGEFGALGPDGNCWPQMAGTVPSEDDEFDWEGDLPL 225

Query: 794  KLPQSDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNEL 973
            K PQ DLVIYEMHVRGFT+HESSN+K+PGTYLG+VEKLD+LK+LGVNC+ELMPCHEFNEL
Sbjct: 226  KYPQKDLVIYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNEL 285

Query: 974  EHYSYNSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIE 1153
            E+Y +NS  GDY++NFWGYSTIN+FSPM RYSSAGI N G   +NE K+L++EAHKRGIE
Sbjct: 286  EYYGHNSAQGDYRVNFWGYSTINYFSPMIRYSSAGIRNCGQDGINEIKFLIKEAHKRGIE 345

Query: 1154 VIMDVVFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFI 1333
            VIMDVVFNHTAEGNENGPI+SFRG+DNS++YMLAPKGE+YNYSGCGNTFNCNHP+VRQFI
Sbjct: 346  VIMDVVFNHTAEGNENGPIISFRGVDNSMYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFI 405

Query: 1334 LDCLR 1348
            +DCLR
Sbjct: 406  VDCLR 410


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  553 bits (1424), Expect = e-155
 Identities = 271/412 (65%), Positives = 315/412 (76%), Gaps = 6/412 (1%)
 Frame = +2

Query: 131  LQLLHYSRIPSYCPPKFTPKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNNASKRGD 310
            ++L HY+   S+  PK           N    G  S  +    + +     +  A   G 
Sbjct: 11   MELTHYASCSSHYLPKLLSPHKPPQWWNPTPRGKSSNNWSAKPSNMRFSKWVVAAVGSGA 70

Query: 311  TETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLRE 490
                VVVEKP   P  F VF G P P GAT RDGGV            TLCLIS SDL E
Sbjct: 71   EAETVVVEKPKLQP--FLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDLEE 128

Query: 491  KRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYA 670
             RVTEQI+L+PLTNKTGDVWHVFLKG+FE++ YGYKFDG FSPE+G Y+DSS++L+DPYA
Sbjct: 129  DRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPYA 188

Query: 671  KAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTR 850
            KAV+SRGE+G LGP+ +CWP MA ++PS++ EFDWEGDLPLK PQ DL+IYEMHVRGFTR
Sbjct: 189  KAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFTR 248

Query: 851  HESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYK------ 1012
            HESS +K+PGTY G+VEKLD+LK+LGVNCIELMPCHEFNELE++SYNSVL DY+      
Sbjct: 249  HESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRYTCISM 308

Query: 1013 MNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEG 1192
            +NFWGYST+N+FSPM RYSSAGI N G  A+NE K L+REAHKRGIEV+MDVVFNHTAEG
Sbjct: 309  VNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEG 368

Query: 1193 NENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            NENGPILSFRG+DNSV+YMLAPKGE+YNYSGCGNTFNCNHPIVRQFILDCLR
Sbjct: 369  NENGPILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLR 420


>dbj|BAF52941.1| isoamylase-type starch-debranching enzyme 1 [Phaseolus vulgaris]
            gi|561033644|gb|ESW32223.1| hypothetical protein
            PHAVU_002G303600g [Phaseolus vulgaris]
          Length = 791

 Score =  552 bits (1423), Expect = e-154
 Identities = 262/402 (65%), Positives = 316/402 (78%), Gaps = 4/402 (0%)
 Frame = +2

Query: 155  IPSYCPPKFT--PKKYLRCRKNKLNFGIPSKTFENTGTKVNPMVILNNASKRG--DTETA 322
            +PS   P FT  P+  L  R +  N+         T + +  +     A   G  DTETA
Sbjct: 6    LPSLSAPTFTILPQCALTVRVSLANYRFSQ--LHETDSVIRTVARTRAARNGGAVDTETA 63

Query: 323  VVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXXXXTLCLISLSDLREKRVT 502
            VV  + P +   F+V  G+ +PFGAT RDGGV            TLCL +LSD +  RVT
Sbjct: 64   VV--EKPQLESLFQVSRGYASPFGATVRDGGVNFAIYSLNAFSATLCLFTLSDFQNNRVT 121

Query: 503  EQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDGFYFDSSKVLIDPYAKAVV 682
            E + L+PL NKTG +WHVFLKGDF DM YGYKFDG FSP +G Y+DSS++++DPYAKAV+
Sbjct: 122  ESVPLDPLINKTGGIWHVFLKGDFSDMLYGYKFDGKFSPLEGHYYDSSRIVLDPYAKAVI 181

Query: 683  SRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQSDLVIYEMHVRGFTRHESS 862
            SRGE+GALGP+ +CWPQMA +VPS +DEFDWEGDLPLK PQ DLV+YEMHVRGFT+HESS
Sbjct: 182  SRGEFGALGPNGNCWPQMAGMVPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESS 241

Query: 863  NSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSYNSVLGDYKMNFWGYSTIN 1042
            N+K+PGTYLG+VEKLD+LK+LGVNC+ELMPCHEFNELE+ SYNSV GDY++NFWGYSTIN
Sbjct: 242  NTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTIN 301

Query: 1043 FFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFR 1222
            FFSPM RYSSAGI N G   +NE K+L++EAHKRGIEVIMDVVFNHTAEGNE+GPI+SFR
Sbjct: 302  FFSPMIRYSSAGIRNCGRDGINEIKFLIKEAHKRGIEVIMDVVFNHTAEGNEDGPIISFR 361

Query: 1223 GLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            G+DNS++YM+APKGE+YNYSGCGNTFNC+HP+VRQFI+DCLR
Sbjct: 362  GVDNSIYYMVAPKGEFYNYSGCGNTFNCSHPVVRQFIVDCLR 403


>ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 794

 Score =  550 bits (1417), Expect = e-154
 Identities = 255/362 (70%), Positives = 300/362 (82%), Gaps = 3/362 (0%)
 Frame = +2

Query: 272  PMVILNNASKRGDTETAV---VVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXX 442
            P V  N+    G T  AV   VVE+ P + RRF+V  G P PFGATP DGGV        
Sbjct: 46   PPVKANDGGGAGTTAEAVETAVVERSPKL-RRFQVSDGRPNPFGATPCDGGVNFAVYAGN 104

Query: 443  XXXXTLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPE 622
                T+CLI+ +DL + +VTEQI L+PL NKTGDVWHVFL GD +D+ YGYKFDG FSPE
Sbjct: 105  AVSATVCLITPADLEQDKVTEQIPLDPLNNKTGDVWHVFLTGDVKDILYGYKFDGKFSPE 164

Query: 623  DGFYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLP 802
            +G Y+D SK+++DPYAKAV+ RGE+G LGP+ +CWPQMAC+VPS +D+F+WEGDLPL  P
Sbjct: 165  EGHYYDPSKIVMDPYAKAVIRRGEFGNLGPNGNCWPQMACMVPSFDDKFNWEGDLPLNYP 224

Query: 803  QSDLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHY 982
            Q DL+IYEMHVRGFTRHESS ++ PGTYLG+VEKLD+LK+LG+NCIELMPCHEFNELE++
Sbjct: 225  QKDLIIYEMHVRGFTRHESSMTELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEYF 284

Query: 983  SYNSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIM 1162
             YNSVLGDYK+N+WGYST+NFFSPM RYSSAG  N G  A+NE K+LV+EAH+RGIEVIM
Sbjct: 285  GYNSVLGDYKVNYWGYSTVNFFSPMIRYSSAGTRNCGRDAINEVKFLVKEAHRRGIEVIM 344

Query: 1163 DVVFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDC 1342
            DVVFNHTAEGNENGPILSFRG DNSV+YMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DC
Sbjct: 345  DVVFNHTAEGNENGPILSFRGADNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDC 404

Query: 1343 LR 1348
            LR
Sbjct: 405  LR 406


>gb|EXC12839.1| Isoamylase 1 [Morus notabilis]
          Length = 805

 Score =  544 bits (1402), Expect = e-152
 Identities = 250/360 (69%), Positives = 295/360 (81%)
 Frame = +2

Query: 269  NPMVILNNASKRGDTETAVVVEKPPAMPRRFEVFSGHPTPFGATPRDGGVXXXXXXXXXX 448
            N   ++  A + G +    VV+  P + R F+V  G P P GAT RDGGV          
Sbjct: 45   NRSELIVKAGRGGGSAEVAVVDGGPQLSR-FQVSRGSPVPLGATLRDGGVNFAVFAGNAV 103

Query: 449  XXTLCLISLSDLREKRVTEQIALNPLTNKTGDVWHVFLKGDFEDMAYGYKFDGSFSPEDG 628
              TLCL+SLSDL + RVTE+I+L+P+ N+TG VWHVF+K DF+DM YGYKFDG FSPE+G
Sbjct: 104  KATLCLMSLSDLEQNRVTEEISLDPVINQTGSVWHVFVKVDFKDMLYGYKFDGKFSPEEG 163

Query: 629  FYFDSSKVLIDPYAKAVVSRGEYGALGPDKDCWPQMACLVPSTNDEFDWEGDLPLKLPQS 808
             Y+D S++++DPY KAV+ RGEYG LGPD +CWPQMAC VP  ++EFDWEGDLPLK PQ 
Sbjct: 164  HYYDPSRIVLDPYGKAVICRGEYGTLGPDGNCWPQMACAVPFFDNEFDWEGDLPLKYPQE 223

Query: 809  DLVIYEMHVRGFTRHESSNSKYPGTYLGMVEKLDYLKKLGVNCIELMPCHEFNELEHYSY 988
            DL++YEMHVRGFTRHESS +++PGTYLG+VEKL +L++LGVNCIELMPCHEFNELE+YSY
Sbjct: 224  DLIVYEMHVRGFTRHESSGTEFPGTYLGVVEKLHHLQELGVNCIELMPCHEFNELEYYSY 283

Query: 989  NSVLGDYKMNFWGYSTINFFSPMARYSSAGIGNYGLGAVNEFKYLVREAHKRGIEVIMDV 1168
            NS +GDYK+NFWGYST+N+FSPM RYSSAG  N G  AVNE K L+REAHKRGIEVIMDV
Sbjct: 284  NSAIGDYKVNFWGYSTVNYFSPMLRYSSAGTHNCGHDAVNELKLLIREAHKRGIEVIMDV 343

Query: 1169 VFNHTAEGNENGPILSFRGLDNSVFYMLAPKGEYYNYSGCGNTFNCNHPIVRQFILDCLR 1348
            VFNHTAEGNENGPILSFRG DNSV+YMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLR
Sbjct: 344  VFNHTAEGNENGPILSFRGADNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLR 403