BLASTX nr result
ID: Rauwolfia21_contig00020423
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rauwolfia21_contig00020423
(1046 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006347839.1| PREDICTED: mitochondrial import inner membra... 325 2e-86
ref|XP_004230147.1| PREDICTED: mitochondrial import inner membra... 311 4e-82
ref|XP_002278528.1| PREDICTED: mitochondrial import inner membra... 283 8e-74
ref|XP_002524608.1| mitochondrial import inner membrane transloc... 282 2e-73
gb|EOX94457.1| Translocase inner membrane subunit 44-1 isoform 3... 280 7e-73
gb|EOX94455.1| Translocase inner membrane subunit 44-2 isoform 1... 280 7e-73
emb|CBI37117.3| unnamed protein product [Vitis vinifera] 273 6e-71
ref|XP_002300777.1| mitochondrial import inner membrane transloc... 261 3e-67
gb|EMJ02189.1| hypothetical protein PRUPE_ppa004998mg [Prunus pe... 257 6e-66
gb|EXC05426.1| Mitochondrial import inner membrane translocase s... 247 5e-63
ref|XP_002307643.1| mitochondrial import inner membrane transloc... 247 5e-63
ref|XP_004289941.1| PREDICTED: mitochondrial import inner membra... 239 2e-60
ref|XP_006443840.1| hypothetical protein CICLE_v10019992mg [Citr... 233 9e-59
ref|XP_006443839.1| hypothetical protein CICLE_v10019992mg [Citr... 233 9e-59
ref|XP_004296689.1| PREDICTED: mitochondrial import inner membra... 231 3e-58
ref|XP_004170047.1| PREDICTED: uncharacterized protein LOC101231... 231 4e-58
ref|XP_006295848.1| hypothetical protein CARUB_v10024977mg [Caps... 224 4e-56
gb|ESW20621.1| hypothetical protein PHAVU_005G002100g [Phaseolus... 222 2e-55
ref|XP_003522818.1| PREDICTED: mitochondrial import inner membra... 217 7e-54
ref|XP_002886284.1| mitochondrial import inner membrane transloc... 216 1e-53
>ref|XP_006347839.1| PREDICTED: mitochondrial import inner membrane translocase subunit
TIM44-2-like [Solanum tuberosum]
Length = 497
Score = 325 bits (832), Expect = 2e-86
Identities = 165/288 (57%), Positives = 206/288 (71%), Gaps = 2/288 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGR--TRLRLTQANGFTGRREFGVFNEFSKKI 360
M SRK++RD +I++QP+FR++LAP+Q +SGR T LR A+G+ G R FGVFNEFSKK+
Sbjct: 1 MTSRKIVRDLFISKQPVFRRILAPQQVVSGRVATNLRFVGADGYLGIRHFGVFNEFSKKV 60
Query: 361 KGEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKV 540
KGEV+ NQEFQQS DLK RTKQTTEQLYKHVDGVWTEAEATAKKV
Sbjct: 61 KGEVDSNQEFQQSVKVLKEKAEELKGVKEDLKTRTKQTTEQLYKHVDGVWTEAEATAKKV 120
Query: 541 YANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXX 720
YAN+EEKISA KEEV+ +F GK++ GS+G SD S+ ES+ SAS
Sbjct: 121 YANVEEKISATKEEVRSSFRTGKEETPGSSGHSDTSGSNTNESSESASAKTQQEQQQQQS 180
Query: 721 XXXDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSH 900
DNAE++F KVK A+S+SSK S AFQK+KEAKP+DLAKKG+ I+ DELKG+ KR
Sbjct: 181 SSSDNAETVFSKVKSAASSLSSKVSPAFQKIKEAKPVDLAKKGYGIILDELKGTPGKRKR 240
Query: 901 LGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
L ++ P+ + S ERS+RTD+ VLPSKQS W KKW+ +KM+GHP+
Sbjct: 241 LEHSAPTQETSSNFERSTRTDVAVLPSKQSKWNKKWEALKEKMQGHPV 288
>ref|XP_004230147.1| PREDICTED: mitochondrial import inner membrane translocase subunit
TIM44-2-like [Solanum lycopersicum]
Length = 498
Score = 311 bits (796), Expect = 4e-82
Identities = 161/289 (55%), Positives = 202/289 (69%), Gaps = 3/289 (1%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGR--TRLRLTQANGFTGRREFGVFNEFSKKI 360
M SRK++RD +I++QP+FR++LAP+Q + GR T LR A+ + G R FGVFNEFSKK+
Sbjct: 1 MTSRKIVRDLFISKQPVFRRILAPQQVVLGRVTTNLRFVGADRYLGIRHFGVFNEFSKKV 60
Query: 361 KGEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKV 540
KGEV+ NQEFQQS DLK RTKQTTEQLYKHVDGVWTEAEATAKKV
Sbjct: 61 KGEVDSNQEFQQSVKVLKEKAEELKGVKEDLKTRTKQTTEQLYKHVDGVWTEAEATAKKV 120
Query: 541 YANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXX 720
YAN+EEKISA KEEV+ +F GK++ GS+G SD + ES+ SAS
Sbjct: 121 YANVEEKISATKEEVRSSFRTGKEETPGSSGHSDTSGKNTNESSESASAKTQQEQQQQQQ 180
Query: 721 XXX-DNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRS 897
DNAE++F KVK A+S+SSK S A QK+KEAKP+DLAKKG+ I+ DELKG+ KR
Sbjct: 181 SFSSDNAETVFSKVKSAASSLSSKVSPAIQKIKEAKPVDLAKKGYGIILDELKGTPGKRK 240
Query: 898 HLGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
L ++ P+ + S ERS+RTD+ VLPSKQS W KKW+ +KM+GHP+
Sbjct: 241 RLEHSAPTQETSSNFERSTRTDVAVLPSKQSKWNKKWEALKEKMQGHPV 289
>ref|XP_002278528.1| PREDICTED: mitochondrial import inner membrane translocase subunit
tim44-like [Vitis vinifera]
Length = 485
Score = 283 bits (724), Expect = 8e-74
Identities = 154/286 (53%), Positives = 191/286 (66%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
M SRKL+RD Y+++QPL LL+ +Q S R LRL NG +G R F VFNEFS KIKG
Sbjct: 1 MGSRKLVRDLYLSKQPLLLHLLSSQQASSAR--LRLISPNGCSGYRGFSVFNEFSNKIKG 58
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
EVNRN EFQQ+ +LK RTKQTTEQLYKHVDGVWTEAEATAKKV A
Sbjct: 59 EVNRNSEFQQTVKELKEKAEELKGVKEELKVRTKQTTEQLYKHVDGVWTEAEATAKKVSA 118
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKISAA E+V+ TFG GKQ+ S S GTS K +D + + S+
Sbjct: 119 NVKEKISAATEDVKETFGKGKQEFSESAGTSAKSEADVNKGSSSSG-----EEKHHKSGP 173
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE+LFGK K +++S K S+AF+KLKEAK +DLAKKG+ IVKDEL + +KR +
Sbjct: 174 SDTAETLFGKFKSSVSTVSPKVSLAFEKLKEAKVLDLAKKGYGIVKDELSSNPSKRKQMQ 233
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
A S+ P ERS++TDIV++PSKQS W KKW+ DKM+GHP+
Sbjct: 234 RAASSASPG---ERSTKTDIVIVPSKQSRWSKKWEALKDKMQGHPV 276
>ref|XP_002524608.1| mitochondrial import inner membrane translocase subunit tim44,
putative [Ricinus communis] gi|223536161|gb|EEF37816.1|
mitochondrial import inner membrane translocase subunit
tim44, putative [Ricinus communis]
Length = 486
Score = 282 bits (721), Expect = 2e-73
Identities = 161/289 (55%), Positives = 196/289 (67%), Gaps = 3/289 (1%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRL--TQANGFT-GRREFGVFNEFSKK 357
MA +KL+RD ++++QPLF L + + RTRLRL T NG G R FGVF+EFSKK
Sbjct: 1 MAGKKLVRDLFLSRQPLFLHLTSQQ---GSRTRLRLPSTLPNGKNMGYRGFGVFSEFSKK 57
Query: 358 IKGEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKK 537
+KGEV R+QEFQQS DL+ RTK+TTEQLYKHVDG WTEAEATAKK
Sbjct: 58 VKGEVTRSQEFQQSVKELKDKAEELKGVKEDLRIRTKETTEQLYKHVDGAWTEAEATAKK 117
Query: 538 VYANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXX 717
V AN++EKISAAKEEV+ T IGKQ+ SGST +SDK +D ++ +S+S
Sbjct: 118 VSANVKEKISAAKEEVKETLNIGKQESSGSTKSSDKDGADVKDDKKSSS----GEETDEQ 173
Query: 718 XXXXDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRS 897
DNAES FG KF +++ SSK S AFQKLKEAK D KKG+++VKDEL G+ +KR
Sbjct: 174 TGSSDNAESFFG--KFKSSTPSSKLSSAFQKLKEAKVTDFVKKGYDVVKDELYGNPSKRK 231
Query: 898 HLGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
HL P PS K E S+RTDIVVLPSKQS W KKW+ F +KM+GHPL
Sbjct: 232 HLEYTPP---PSFKGETSTRTDIVVLPSKQSRWNKKWEAFREKMQGHPL 277
>gb|EOX94457.1| Translocase inner membrane subunit 44-1 isoform 3 [Theobroma cacao]
Length = 461
Score = 280 bits (716), Expect = 7e-73
Identities = 155/286 (54%), Positives = 192/286 (67%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA RKL+RDF+IA+QPLF + + RLRL NG++ R F VFNEFS KIKG
Sbjct: 1 MAGRKLVRDFFIARQPLFLNFTSQQ---GSNVRLRLLSGNGYSSNRRFSVFNEFSNKIKG 57
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E NRN EF+QS +LK RTKQTT+QLYK VDG WTEAEATAKKV A
Sbjct: 58 EANRNPEFKQSVEELKGKAE-------ELKVRTKQTTQQLYKQVDGAWTEAEATAKKVSA 110
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
NM+EKISAAKEEV+ TFG+GK++ S TGTS + +D ++ +++S
Sbjct: 111 NMKEKISAAKEEVKETFGVGKEEFSEGTGTSAQHGADVKDGDKASS----GEQKHEQSAS 166
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE++FGK K G + S K S+AFQ+LKEAK +DLAKKG++IVKDEL G+ +KR HL
Sbjct: 167 GDTAETIFGKFKSGIS--SPKVSLAFQRLKEAKVVDLAKKGYDIVKDELSGNPSKRRHLE 224
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
P PS ERS+RTDIVV+PSKQS W KKW+ F +KM+GHPL
Sbjct: 225 YTPP---PSSTGERSTRTDIVVMPSKQSRWSKKWEAFKEKMQGHPL 267
>gb|EOX94455.1| Translocase inner membrane subunit 44-2 isoform 1 [Theobroma cacao]
Length = 476
Score = 280 bits (716), Expect = 7e-73
Identities = 155/286 (54%), Positives = 192/286 (67%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA RKL+RDF+IA+QPLF + + RLRL NG++ R F VFNEFS KIKG
Sbjct: 1 MAGRKLVRDFFIARQPLFLNFTSQQ---GSNVRLRLLSGNGYSSNRRFSVFNEFSNKIKG 57
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E NRN EF+QS +LK RTKQTT+QLYK VDG WTEAEATAKKV A
Sbjct: 58 EANRNPEFKQSVEELKGKAE-------ELKVRTKQTTQQLYKQVDGAWTEAEATAKKVSA 110
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
NM+EKISAAKEEV+ TFG+GK++ S TGTS + +D ++ +++S
Sbjct: 111 NMKEKISAAKEEVKETFGVGKEEFSEGTGTSAQHGADVKDGDKASS----GEQKHEQSAS 166
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE++FGK K G + S K S+AFQ+LKEAK +DLAKKG++IVKDEL G+ +KR HL
Sbjct: 167 GDTAETIFGKFKSGIS--SPKVSLAFQRLKEAKVVDLAKKGYDIVKDELSGNPSKRRHLE 224
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
P PS ERS+RTDIVV+PSKQS W KKW+ F +KM+GHPL
Sbjct: 225 YTPP---PSSTGERSTRTDIVVMPSKQSRWSKKWEAFKEKMQGHPL 267
>emb|CBI37117.3| unnamed protein product [Vitis vinifera]
Length = 484
Score = 273 bits (699), Expect = 6e-71
Identities = 152/286 (53%), Positives = 188/286 (65%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
M SRKL+RD Y+++QPL LL+ +Q S R LRL NG +G R F VFNEFS KIKG
Sbjct: 1 MGSRKLVRDLYLSKQPLLLHLLSSQQASSAR--LRLISPNGCSGYRGFSVFNEFSNKIKG 58
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
EVNRN EFQQ+ +LK RTKQTTEQLYKHVDGVWTEAEATAKKV A
Sbjct: 59 EVNRNSEFQQTVKELKEKAEELKGVKEELKVRTKQTTEQLYKHVDGVWTEAEATAKKVSA 118
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKISAA E+V+ TFG GKQ+ S S GTS K +D + + S+
Sbjct: 119 NVKEKISAATEDVKETFGKGKQEFSESAGTSAKSEADVNKGSSSSG-----EEKHHKSGP 173
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE+LFGK K +++S K S+AF+KLKEAK +DLAKKG+ IVKDEL + +KR +
Sbjct: 174 SDTAETLFGKFKSSVSTVSPKVSLAFEKLKEAKVLDLAKKGYGIVKDELSSNPSKRKQMQ 233
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
A S+ P ERS++TDIV++PSKQS W KKW+ DK HP+
Sbjct: 234 RAASSASPG---ERSTKTDIVIVPSKQSRWSKKWEALKDK-ASHPV 275
>ref|XP_002300777.1| mitochondrial import inner membrane translocase subunit TIM44 family
protein [Populus trichocarpa] gi|222842503|gb|EEE80050.1|
mitochondrial import inner membrane translocase subunit
TIM44 family protein [Populus trichocarpa]
Length = 484
Score = 261 bits (667), Expect = 3e-67
Identities = 150/288 (52%), Positives = 192/288 (66%), Gaps = 2/288 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFT--GRREFGVFNEFSKKI 360
MASRKL+RD ++++QPLF +L + + +SGR RL++ +G T G R F VFNEFS K+
Sbjct: 1 MASRKLVRDLFLSRQPLFLRLTS--KQVSGR-RLQVLSNHGCTYYGYRRFSVFNEFSNKV 57
Query: 361 KGEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKV 540
KGE +RNQEF+QS +LK RTKQTTEQLYKHVDGVWTEAEATAKKV
Sbjct: 58 KGEADRNQEFKQSVKELKEKAEELKGAKEELKVRTKQTTEQLYKHVDGVWTEAEATAKKV 117
Query: 541 YANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXX 720
AN++EKISAA EEV+ TFGIGK+ S S GTS K + EE ++++
Sbjct: 118 SANVKEKISAATEEVKETFGIGKES-SESAGTSAKDGAGAEEGTKAST----GEEADKQT 172
Query: 721 XXXDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSH 900
D AES FG KF + SS S A+QKLKEA+ ++ KKG+++VKDEL G+ KR H
Sbjct: 173 GTGDTAESFFG--KFKSRIPSSNVSSAYQKLKEARVSEMMKKGYDVVKDELYGNTNKRKH 230
Query: 901 LGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
L + P P+ E S++TD+VVLPSKQS W KKW+ F +KM+GHPL
Sbjct: 231 LEHTPP---PAFSGEISTKTDVVVLPSKQSRWSKKWEAFREKMQGHPL 275
>gb|EMJ02189.1| hypothetical protein PRUPE_ppa004998mg [Prunus persica]
Length = 482
Score = 257 bits (656), Expect = 6e-66
Identities = 145/286 (50%), Positives = 187/286 (65%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA+RKL+RD ++ +QPLF QL +Q S TR+RL ANG++G R F VFNEFSK++KG
Sbjct: 1 MAARKLVRDLHLYRQPLFHQLTLQQQVSS--TRIRLISANGYSGSRRFSVFNEFSKQVKG 58
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E +N EFQQS DLK RTKQTTEQLYK VDG WTEAEA AKKV A
Sbjct: 59 EATKNPEFQQSVKELKEKAEELKGVKEDLKVRTKQTTEQLYKQVDGAWTEAEAAAKKVSA 118
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKI+AA EEV+GTFGIG + SG +G + +D E+ +++S
Sbjct: 119 NVKEKITAATEEVKGTFGIGNEQASGPSGAKSENFADAEDGTKASS----GEQKHQESGS 174
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
+ E+LFG KF +++ SS S AF ++KE K ID+AKKG++IVKDEL G+ KR HL
Sbjct: 175 SNTEETLFG--KFRSSASSSYFSSAFSRVKEVKFIDMAKKGYDIVKDELSGNPTKRKHL- 231
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
PS+ P +VE S+RTDIV +P QS W KKW+ F KM+GHP+
Sbjct: 232 EYDPSTTP--QVEVSTRTDIVAVP--QSRWSKKWEAFRMKMQGHPI 273
>gb|EXC05426.1| Mitochondrial import inner membrane translocase subunit TIM44 [Morus
notabilis]
Length = 481
Score = 247 bits (631), Expect = 5e-63
Identities = 141/286 (49%), Positives = 180/286 (62%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA RKL+RD+ +++ LF QL + S +RL L ANGF+G R F VFNEFSK++KG
Sbjct: 1 MAGRKLVRDYLLSKSRLFLQLTHSQPQASS-SRLLLPPANGFSGNRRFSVFNEFSKQVKG 59
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E N+N EFQQS DLK RTKQTTEQLYK VDG WTEAEA AK V A
Sbjct: 60 EANKNSEFQQSVKELKDKARELKGVKEDLKVRTKQTTEQLYKQVDGAWTEAEAKAKMVSA 119
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKISAA E+V+GT GIGK++ +G++ + E+ + S
Sbjct: 120 NLKEKISAATEDVKGTLGIGKEEAAGTSAKTG-------ENVKDGSKASDGEQATKQSDS 172
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE+LFGK K + +S AS AFQ+ KEAK IDLAKKG++IVKDEL + KR HL
Sbjct: 173 DDTAETLFGKYK--SRISTSNASSAFQRFKEAKFIDLAKKGYDIVKDELSSNPKKRKHLQ 230
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
+ SS KVERS+RT++V +PSKQS KKW+ F +K+ HP+
Sbjct: 231 HDPSSS----KVERSTRTEVVFVPSKQSPLNKKWEAFKNKVLSHPI 272
>ref|XP_002307643.1| mitochondrial import inner membrane translocase subunit TIM44 family
protein [Populus trichocarpa] gi|222857092|gb|EEE94639.1|
mitochondrial import inner membrane translocase subunit
TIM44 family protein [Populus trichocarpa]
Length = 485
Score = 247 bits (631), Expect = 5e-63
Identities = 142/286 (49%), Positives = 180/286 (62%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MASRKL+RD +++QPLF ++ +Q S R ++ ++G R F VFNEFSKK+KG
Sbjct: 1 MASRKLVRDLVLSRQPLFLHFIS-KQVPSSRLQVVSNYGCPYSGYRRFSVFNEFSKKVKG 59
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E N EF+QS +LK RTKQTTE+LYKHVD VWTEAEATAKKV A
Sbjct: 60 EAQINLEFKQSVKELKEKAEELKGVKEELKVRTKQTTEKLYKHVDCVWTEAEATAKKVSA 119
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EK+SAA EEV+ TFGIGK++ S S GTS K EE ++
Sbjct: 120 NVKEKVSAATEEVKETFGIGKEESSESAGTSAKDGVGAEECRKA----FPGEEADKQTGT 175
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE+LFG KF ++ SSK S AFQKLKEA+ ++ KKG+++VKDEL G+ R HL
Sbjct: 176 SDTAETLFG--KFKSSIPSSKVSSAFQKLKEARVSEMMKKGYDVVKDELYGNTNTRKHLE 233
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
P PS E S++TDI VLPSKQS W KKW+ +KM+GHPL
Sbjct: 234 YTPP---PSFSGEISTKTDIAVLPSKQSRWSKKWEAIREKMQGHPL 276
>ref|XP_004289941.1| PREDICTED: mitochondrial import inner membrane translocase subunit
TIM44-2-like [Fragaria vesca subsp. vesca]
Length = 482
Score = 239 bits (609), Expect = 2e-60
Identities = 136/286 (47%), Positives = 182/286 (63%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA RKL+RD ++ +Q L +L +Q SG T +RL ANG++G R F VFNEFSK++K
Sbjct: 1 MAGRKLVRDLFLHRQSL---VLRSQQQASG-TNIRLLSANGYSGNRRFSVFNEFSKQVKE 56
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E N+N F+++ DLK RTKQTTEQLYK VDG WTEAEATAKKV A
Sbjct: 57 ETNKNPHFKKTVKELKKQVEELKGVKEDLKVRTKQTTEQLYKQVDGAWTEAEATAKKVSA 116
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EK SAA EEV+ +FGIGK+ SG + S +D + ++++S
Sbjct: 117 NVKEKFSAATEEVKESFGIGKEQASGPSRASADHVADEKGGSKASS----TEDKHQHSGA 172
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D AE+LFG KF + SS S + KEAK +D+AK+G+ IVKDEL GS +++ HLG
Sbjct: 173 SDTAETLFG--KFRSKVSSSNVSSVLNRFKEAKFVDMAKRGYEIVKDELNGSQSRKKHLG 230
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
PSS P +VERS+ +DI ++P+KQS W KKW+ F KM+G+PL
Sbjct: 231 -FDPSSIP--RVERSATSDIAIVPTKQSPWSKKWEAFKMKMQGNPL 273
>ref|XP_006443840.1| hypothetical protein CICLE_v10019992mg [Citrus clementina]
gi|557546102|gb|ESR57080.1| hypothetical protein
CICLE_v10019992mg [Citrus clementina]
Length = 409
Score = 233 bits (594), Expect = 9e-59
Identities = 147/288 (51%), Positives = 186/288 (64%), Gaps = 2/288 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQ-PLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIK 363
MASRKL+RD ++++Q P F QL P Q S TRLRL ANG++ R+F VF EFSKKIK
Sbjct: 1 MASRKLVRDLFLSRQRPRFLQL-TPLQGSS--TRLRLVSANGYSSNRQFSVFKEFSKKIK 57
Query: 364 GEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVY 543
GE N EF+ S +LK RTKQTTEQLYK VDGVW EAE+T KKV
Sbjct: 58 GEAESNPEFKHSVKELKKKAEEIKGVKEELKERTKQTTEQLYKQVDGVWMEAESTVKKVS 117
Query: 544 ANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXX 723
A+M+EKISAA EEV+GTF +G+T TS K + D + +++S
Sbjct: 118 ASMKEKISAATEEVKGTF------RTGTTDTSAKHDDDVRDGFKASS----GEEKQKQTV 167
Query: 724 XXDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSH 900
D AE+ +GK+K +SISS K ++AFQKLKEAK +DLAKKG++IVKDEL GS +KR H
Sbjct: 168 SSDTAETFYGKLK---SSISSPKFTLAFQKLKEAKVVDLAKKGYDIVKDELSGSPSKRKH 224
Query: 901 LGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
L TPS PS E+S+RTD+VV PSK+S W K +KM+G+P+
Sbjct: 225 L-EYTPS--PSWTGEKSTRTDLVVTPSKKSMWSK----LKEKMQGYPV 265
>ref|XP_006443839.1| hypothetical protein CICLE_v10019992mg [Citrus clementina]
gi|568851735|ref|XP_006479542.1| PREDICTED: mitochondrial
import inner membrane translocase subunit TIM44-2-like
[Citrus sinensis] gi|557546101|gb|ESR57079.1|
hypothetical protein CICLE_v10019992mg [Citrus
clementina]
Length = 474
Score = 233 bits (594), Expect = 9e-59
Identities = 147/288 (51%), Positives = 186/288 (64%), Gaps = 2/288 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQ-PLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIK 363
MASRKL+RD ++++Q P F QL P Q S TRLRL ANG++ R+F VF EFSKKIK
Sbjct: 1 MASRKLVRDLFLSRQRPRFLQL-TPLQGSS--TRLRLVSANGYSSNRQFSVFKEFSKKIK 57
Query: 364 GEVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVY 543
GE N EF+ S +LK RTKQTTEQLYK VDGVW EAE+T KKV
Sbjct: 58 GEAESNPEFKHSVKELKKKAEEIKGVKEELKERTKQTTEQLYKQVDGVWMEAESTVKKVS 117
Query: 544 ANMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXX 723
A+M+EKISAA EEV+GTF +G+T TS K + D + +++S
Sbjct: 118 ASMKEKISAATEEVKGTF------RTGTTDTSAKHDDDVRDGFKASS----GEEKQKQTV 167
Query: 724 XXDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSH 900
D AE+ +GK+K +SISS K ++AFQKLKEAK +DLAKKG++IVKDEL GS +KR H
Sbjct: 168 SSDTAETFYGKLK---SSISSPKFTLAFQKLKEAKVVDLAKKGYDIVKDELSGSPSKRKH 224
Query: 901 LGNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
L TPS PS E+S+RTD+VV PSK+S W K +KM+G+P+
Sbjct: 225 L-EYTPS--PSWTGEKSTRTDLVVTPSKKSMWSK----LKEKMQGYPV 265
>ref|XP_004296689.1| PREDICTED: mitochondrial import inner membrane translocase subunit
TIM44-2-like [Fragaria vesca subsp. vesca]
Length = 480
Score = 231 bits (590), Expect = 3e-58
Identities = 131/286 (45%), Positives = 180/286 (62%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA+RKL+RD ++ QP+F +SG T++RL NG++G R + VFNEFSK++K
Sbjct: 1 MAARKLVRDLFLHSQPIFLH-----SQVSG-TKIRLLLGNGYSGNRRYSVFNEFSKQVKE 54
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E N+N +F+++ DLK RTKQTTEQLYK VDGVWTEAEA AKKV A
Sbjct: 55 ETNKNPQFKKTVKELKEQVEELKGVKEDLKVRTKQTTEQLYKQVDGVWTEAEAAAKKVSA 114
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKI AA EEV+G+FGIGK+ S G S +D ++ +++AS
Sbjct: 115 NVKEKILAATEEVKGSFGIGKEQASEPNGASADHVADEKDGSKTAS----TEDKHQRLGS 170
Query: 727 XDNAESLFGKVKFGAASISSKASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHLG 906
D E+LFG KF + SS S F + KEAK ID+AKKG++I+KDEL + K+ HLG
Sbjct: 171 SDTTETLFG--KFRSRISSSNVSSVFHRFKEAKVIDMAKKGYDILKDELNDNPIKKKHLG 228
Query: 907 NATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
PSS P +VE+S+ +D+ ++P+KQS KKW+ F KM+ +PL
Sbjct: 229 -FDPSSIP--RVEKSATSDVAIVPTKQSRLRKKWEAFRMKMQANPL 271
>ref|XP_004170047.1| PREDICTED: uncharacterized protein LOC101231997 [Cucumis sativus]
Length = 484
Score = 231 bits (589), Expect = 4e-58
Identities = 135/287 (47%), Positives = 178/287 (62%), Gaps = 1/287 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA RKL+RD +++Q + Q + + S TR +L +G++ R F VFNEFSKK+KG
Sbjct: 1 MAGRKLVRDILLSRQSIVYQFTS---HGSSCTRSQLLLPSGYSINRRFSVFNEFSKKVKG 57
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E ++N EFQQS DLK RTKQTTEQ+YK VDGVW+EAEATA+KV A
Sbjct: 58 EADKNTEFQQSVKELKEKAEELKGVKEDLKVRTKQTTEQIYKQVDGVWSEAEATARKVSA 117
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
+++EK+SAA EEV+ FG ++ SGST +S +D ++ AS
Sbjct: 118 DVKEKLSAATEEVKEAFGFSPKNSSGSTCSSTDHGADAKKHGSEAS---SENAKDQHPGS 174
Query: 727 XDNAESLFGKVKFGAASISSKA-SMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHL 903
++E+ FGK K +SI S S AF++LK K IDLAK+G IVKDEL G K+ HL
Sbjct: 175 SGSSETFFGKFK---SSIPSPGISSAFERLKSTKLIDLAKRGCEIVKDELSGKPHKKKHL 231
Query: 904 GNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
+S KVERS+RTD+VVLPSKQS W KKW+ F +KM+GHP+
Sbjct: 232 EYEASASP---KVERSTRTDVVVLPSKQSRWSKKWEAFREKMQGHPV 275
>ref|XP_006295848.1| hypothetical protein CARUB_v10024977mg [Capsella rubella]
gi|482564556|gb|EOA28746.1| hypothetical protein
CARUB_v10024977mg [Capsella rubella]
Length = 473
Score = 224 bits (571), Expect = 4e-56
Identities = 129/287 (44%), Positives = 178/287 (62%), Gaps = 1/287 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MASRKL+RD I +QP+F+QL+ R+ RL L Q NG+ R F VF+EFSKKI+G
Sbjct: 1 MASRKLIRDLLITKQPIFQQLVHQRRV---GARLGLLQGNGYASHRLFSVFSEFSKKIRG 57
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E + N EFQ++ DLK RTKQTTEQLYK VDGVWTEAE+ AKKV +
Sbjct: 58 EADSNPEFQKTVKELKERAQELKSVKEDLKVRTKQTTEQLYKQVDGVWTEAESVAKKVSS 117
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
++++K+SAA EEV+ +F +GK++ + S +S ++ E+ +S +
Sbjct: 118 SVKDKLSAATEEVKESFKLGKEENAESASSSGTGTTEGEKQQQSGT-------------- 163
Query: 727 XDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHL 903
+ + FGK K +SISS S A KLKEAKP+D AKKG +IVKDEL G+ +KR HL
Sbjct: 164 TEEQHTFFGKFK---SSISSPMLSEALHKLKEAKPLDFAKKGLDIVKDELHGNPSKRKHL 220
Query: 904 GNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
P+ ERS+RT++V+ +KQS W KKW++ KM+G+P+
Sbjct: 221 EYTPPA---PFTGERSTRTEMVITSTKQSKWQKKWESLRQKMQGYPV 264
>gb|ESW20621.1| hypothetical protein PHAVU_005G002100g [Phaseolus vulgaris]
Length = 479
Score = 222 bits (566), Expect = 2e-55
Identities = 134/287 (46%), Positives = 177/287 (61%), Gaps = 1/287 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA+RKL+RDF+++++ L R + R+R + RR + VFNEFSKKIKG
Sbjct: 1 MATRKLIRDFFLSERSLLLPSPHHRHKVWNRSRRFPLD---WEDRRSYSVFNEFSKKIKG 57
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E RNQEFQQS +LK RTKQ TE+LYK VD VWTEAEA AKKV
Sbjct: 58 EAVRNQEFQQSVKELKEKADELKGVKEELKERTKQKTEKLYKQVDEVWTEAEAAAKKVSY 117
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKISAA EEV+GTFGIGKQD SGST +S K +D +++S
Sbjct: 118 NVKEKISAATEEVKGTFGIGKQDSSGSTDSSTKQGADVNGGNKTSS----QEEKNQQSGS 173
Query: 727 XDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHL 903
+ +SLFGK K ++ISS S AFQKLK+AK +D+ KK ++IVK+EL + KR +
Sbjct: 174 SNATDSLFGKFK---STISSPNVSAAFQKLKDAKLVDITKKSYDIVKEELSSTPTKRKRV 230
Query: 904 GNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
A+ E S+RTD+VV+PS+QS W KK+D F +K++GHP+
Sbjct: 231 PFASSG-------ETSTRTDLVVMPSQQSWWSKKFDEFREKVKGHPV 270
>ref|XP_003522818.1| PREDICTED: mitochondrial import inner membrane translocase subunit
TIM44-2-like [Glycine max]
Length = 473
Score = 217 bits (552), Expect = 7e-54
Identities = 133/287 (46%), Positives = 172/287 (59%), Gaps = 1/287 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA+RKL+RD ++++ L Q S RL L + RR + VFNEFSK IKG
Sbjct: 1 MATRKLIRDLLLSKRSLLHH-----QGWSRSRRLPLV----WEDRRRYSVFNEFSKNIKG 51
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
+ RNQEFQQS +LK RTKQ TE+LYK VD WTEAEA A+KV
Sbjct: 52 QAVRNQEFQQSVKELKEKADELKGVKEELKERTKQKTEKLYKQVDEAWTEAEAAARKVSY 111
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
N++EKISAA EEV+ TFGIGKQD SGST +S K +D A +
Sbjct: 112 NVKEKISAASEEVKETFGIGKQDSSGSTDSSTKQGAD----ANGGNQTSREEEKNQQSGS 167
Query: 727 XDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHL 903
+A+SLFGK K ++ISS S AFQKLK+AK +D+ KKG++IVK+EL + KR +
Sbjct: 168 SKDADSLFGKFK---STISSPNVSAAFQKLKDAKLVDITKKGYDIVKEELSSTPTKRKRV 224
Query: 904 GNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
A+ E S+RTD+VV+PSKQS W KK+D F +K++GHP+
Sbjct: 225 PFASSG-------ETSTRTDLVVMPSKQSWWSKKFDEFREKVKGHPV 264
>ref|XP_002886284.1| mitochondrial import inner membrane translocase subunit TIM44
[Arabidopsis lyrata subsp. lyrata]
gi|297332124|gb|EFH62543.1| mitochondrial import inner
membrane translocase subunit TIM44 [Arabidopsis lyrata
subsp. lyrata]
Length = 477
Score = 216 bits (550), Expect = 1e-53
Identities = 124/287 (43%), Positives = 171/287 (59%), Gaps = 1/287 (0%)
Frame = +1
Query: 187 MASRKLLRDFYIAQQPLFRQLLAPRQNLSGRTRLRLTQANGFTGRREFGVFNEFSKKIKG 366
MA+RK++RD I +QPL RQL R L G TR A G++ R F VF+EFSK I+G
Sbjct: 1 MATRKIIRDLLITKQPLLRQLFHQRV-LRGNTRSEFLPAIGYSSHRRFSVFSEFSKNIRG 59
Query: 367 EVNRNQEFQQSXXXXXXXXXXXXXXXXDLKARTKQTTEQLYKHVDGVWTEAEATAKKVYA 546
E + N EFQ++ DLK RTKQTT+QLYK VDGVWTEAE+ KKV +
Sbjct: 60 EADSNPEFQRTVKEFKERAEEFKGVKEDLKVRTKQTTDQLYKQVDGVWTEAESAPKKVSS 119
Query: 547 NMEEKISAAKEEVQGTFGIGKQDPSGSTGTSDKPNSDREESARSASXXXXXXXXXXXXXX 726
++++K+SAA EEV+ +F +GK++ + S +S S E+ S
Sbjct: 120 SVKDKLSAATEEVKESFKLGKEESAESASSSGTRTSQGEKQQHQQS------------GS 167
Query: 727 XDNAESLFGKVKFGAASISS-KASMAFQKLKEAKPIDLAKKGFNIVKDELKGSAAKRSHL 903
+ + F K K +S+SS K S AF KLKEAKP D+ KK +IVKDEL+G+ +++ L
Sbjct: 168 TEELHTFFAKFK---SSLSSPKVSEAFYKLKEAKPFDIVKKALDIVKDELRGNPSRKKFL 224
Query: 904 GNATPSSDPSLKVERSSRTDIVVLPSKQSNWGKKWDTFMDKMRGHPL 1044
P P ERS RT++VV P+KQS W +KW++F +KM+G P+
Sbjct: 225 EYTPP---PPFTGERSMRTEMVVTPTKQSKWQQKWESFKEKMQGSPV 268