BLASTX nr result
ID: Rauwolfia21_contig00011282
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rauwolfia21_contig00011282
(334 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI31361.3| unnamed protein product [Vitis vinifera] 173 2e-41
ref|XP_002277122.1| PREDICTED: queuine tRNA-ribosyltransferase s... 173 2e-41
emb|CAN82729.1| hypothetical protein VITISV_035707 [Vitis vinifera] 173 2e-41
ref|XP_004251975.1| PREDICTED: queuine tRNA-ribosyltransferase s... 167 2e-39
ref|XP_006344948.1| PREDICTED: queuine tRNA-ribosyltransferase s... 165 5e-39
gb|EMJ25715.1| hypothetical protein PRUPE_ppa023702mg [Prunus pe... 162 4e-38
ref|XP_004138668.1| PREDICTED: queuine tRNA-ribosyltransferase s... 159 5e-37
gb|EOY17045.1| Uncharacterized protein isoform 2, partial [Theob... 158 8e-37
gb|EOY17044.1| Uncharacterized protein isoform 1 [Theobroma cacao] 158 8e-37
ref|XP_002526400.1| queuine tRNA-ribosyltransferase, putative [R... 156 3e-36
ref|XP_006478264.1| PREDICTED: queuine tRNA-ribosyltransferase s... 155 5e-36
ref|XP_004299874.1| PREDICTED: queuine tRNA-ribosyltransferase s... 155 7e-36
ref|XP_002325954.2| hypothetical protein POPTR_0019s10480g [Popu... 152 4e-35
gb|ESW05872.1| hypothetical protein PHAVU_011G216600g [Phaseolus... 152 6e-35
ref|XP_004516911.1| PREDICTED: queuine tRNA-ribosyltransferase s... 151 1e-34
ref|XP_006836801.1| hypothetical protein AMTR_s00099p00022390 [A... 148 6e-34
gb|EXB81481.1| Queuine tRNA-ribosyltransferase subunit qtrtd1 [M... 148 8e-34
ref|XP_004983407.1| PREDICTED: queuine tRNA-ribosyltransferase s... 148 8e-34
ref|XP_003605424.1| Queuine tRNA-ribosyltransferase [Medicago tr... 148 8e-34
tpg|DAA46703.1| TPA: hypothetical protein ZEAMMB73_649296 [Zea m... 147 1e-33
>emb|CBI31361.3| unnamed protein product [Vitis vinifera]
Length = 436
Score = 173 bits (439), Expect = 2e-41
Identities = 82/110 (74%), Positives = 100/110 (90%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
LDGIS+KTIS++GG+HQMLGL E+ AAVPRDSI+ LPE +STN+ GASFETP GRRLIK
Sbjct: 68 LDGISSKTISNIGGLHQMLGLHEYGFAAVPRDSIVCLPECDSTNKIGASFETPSGRRLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEYM++ISSMKP + +LADEVP+WVS+KRN+ASVDRTLRWLD+CI+L+
Sbjct: 128 PVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLS 177
>ref|XP_002277122.1| PREDICTED: queuine tRNA-ribosyltransferase subunit QTRTD1 [Vitis
vinifera]
Length = 399
Score = 173 bits (439), Expect = 2e-41
Identities = 82/110 (74%), Positives = 100/110 (90%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
LDGIS+KTIS++GG+HQMLGL E+ AAVPRDSI+ LPE +STN+ GASFETP GRRLIK
Sbjct: 68 LDGISSKTISNIGGLHQMLGLHEYGFAAVPRDSIVCLPECDSTNKIGASFETPSGRRLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEYM++ISSMKP + +LADEVP+WVS+KRN+ASVDRTLRWLD+CI+L+
Sbjct: 128 PVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLS 177
>emb|CAN82729.1| hypothetical protein VITISV_035707 [Vitis vinifera]
Length = 383
Score = 173 bits (439), Expect = 2e-41
Identities = 82/110 (74%), Positives = 100/110 (90%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
LDGIS+KTIS++GG+HQMLGL E+ AAVPRDSI+ LPE +STN+ GASFETP GRRLIK
Sbjct: 68 LDGISSKTISNIGGLHQMLGLHEYGFAAVPRDSIVCLPECDSTNKIGASFETPSGRRLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEYM++ISSMKP + +LADEVP+WVS+KRN+ASVDRTLRWLD+CI+L+
Sbjct: 128 PVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLS 177
>ref|XP_004251975.1| PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like
[Solanum lycopersicum]
Length = 399
Score = 167 bits (422), Expect = 2e-39
Identities = 74/110 (67%), Positives = 98/110 (89%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+GIS K IS++GG+H +LGL +HI+ AVPRDSIIS+P+++STNR GASFETPCGR LIK
Sbjct: 69 LEGISLKAISNIGGLHPLLGLHDHIMVAVPRDSIISIPDHDSTNRIGASFETPCGRMLIK 128
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +YM++ISSM+P++ +LADEVP+WVS KRN+ASV+RTL+WLD+CI+ N
Sbjct: 129 PADYMEMISSMRPNLWATLADEVPAWVSEKRNKASVERTLKWLDECITSN 178
>ref|XP_006344948.1| PREDICTED: queuine tRNA-ribosyltransferase subunit QTRTD1-like
[Solanum tuberosum]
Length = 398
Score = 165 bits (418), Expect = 5e-39
Identities = 73/110 (66%), Positives = 97/110 (88%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+GIS K IS++GG+H +LGL +HI+ AVPRDSIIS+P+++STNR GASFETPCGR LIK
Sbjct: 68 LEGISLKAISNIGGLHPLLGLHDHIMVAVPRDSIISIPDHDSTNRIGASFETPCGRMLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +YM++ISSM+P++ +LADEVP+W S KRN+ASV+RTL+WLD+CI+ N
Sbjct: 128 PADYMEMISSMRPNLWATLADEVPAWASEKRNKASVERTLKWLDECITSN 177
>gb|EMJ25715.1| hypothetical protein PRUPE_ppa023702mg [Prunus persica]
Length = 395
Score = 162 bits (410), Expect = 4e-38
Identities = 74/110 (67%), Positives = 94/110 (85%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S +TIS +GG+HQMLGL EH +PRDSI LPE S+N+ GASFETPCGRRLIK
Sbjct: 68 LEGLSPQTISKIGGLHQMLGLPEHGFVCIPRDSIQCLPECNSSNKFGASFETPCGRRLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEY++LISS++P++ +LADEVP+WVS KRN+ SVDRT+RWLD+CI+LN
Sbjct: 128 PVEYVELISSLRPNIWTTLADEVPTWVSEKRNKTSVDRTIRWLDECIALN 177
>ref|XP_004138668.1| PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like
[Cucumis sativus] gi|449490166|ref|XP_004158527.1|
PREDICTED: queuine tRNA-ribosyltransferase subunit
qtrtd1-like [Cucumis sativus]
Length = 399
Score = 159 bits (401), Expect = 5e-37
Identities = 74/110 (67%), Positives = 95/110 (86%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G S+KTIS++GG+ +LGL E+ AAV RDS+ SLPE ++TN+ GASFET CGRRLI+
Sbjct: 68 LEGPSSKTISNIGGLQHLLGLHEYGFAAVARDSVQSLPECDATNKFGASFETTCGRRLIR 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEY+++ISSMKPD+ SLADEVPSWVS KRN+ SVDRT++WLD+CI+LN
Sbjct: 128 PVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECIALN 177
>gb|EOY17045.1| Uncharacterized protein isoform 2, partial [Theobroma cacao]
Length = 340
Score = 158 bits (399), Expect = 8e-37
Identities = 73/107 (68%), Positives = 90/107 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S KTIS +GG+HQ+LGL E+ AVPRDSI LPE TN+ GASFETPCGR LIK
Sbjct: 1 LEGLSIKTISKIGGLHQLLGLHEYGFVAVPRDSIQCLPEANGTNKLGASFETPCGRLLIK 60
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCI 322
PVEYM++ISSMKP++ +LADEVP+WVS KRN+ SVDRT++WLD+CI
Sbjct: 61 PVEYMEMISSMKPELWATLADEVPAWVSDKRNKTSVDRTIKWLDECI 107
>gb|EOY17044.1| Uncharacterized protein isoform 1 [Theobroma cacao]
Length = 405
Score = 158 bits (399), Expect = 8e-37
Identities = 73/107 (68%), Positives = 90/107 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S KTIS +GG+HQ+LGL E+ AVPRDSI LPE TN+ GASFETPCGR LIK
Sbjct: 68 LEGLSIKTISKIGGLHQLLGLHEYGFVAVPRDSIQCLPEANGTNKLGASFETPCGRLLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCI 322
PVEYM++ISSMKP++ +LADEVP+WVS KRN+ SVDRT++WLD+CI
Sbjct: 128 PVEYMEMISSMKPELWATLADEVPAWVSDKRNKTSVDRTIKWLDECI 174
>ref|XP_002526400.1| queuine tRNA-ribosyltransferase, putative [Ricinus communis]
gi|223534262|gb|EEF35976.1| queuine
tRNA-ribosyltransferase, putative [Ricinus communis]
Length = 406
Score = 156 bits (394), Expect = 3e-36
Identities = 71/110 (64%), Positives = 93/110 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S KTIS +GG+HQM+GL + AVPRDSI LPE STN+ GASFETPCGRRLIK
Sbjct: 103 LEGVSRKTISHIGGLHQMVGLHDSAFVAVPRDSIQCLPECGSTNKFGASFETPCGRRLIK 162
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P EY+++ISS++P++ +LADEVP+WVS KRN+ SV+RT++WLD+CISL+
Sbjct: 163 PSEYLQMISSIRPNIWATLADEVPAWVSDKRNKTSVERTVKWLDECISLS 212
>ref|XP_006478264.1| PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like
[Citrus sinensis]
Length = 397
Score = 155 bits (392), Expect = 5e-36
Identities = 72/107 (67%), Positives = 92/107 (85%)
Frame = +2
Query: 5 DGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIKP 184
+G S KTIS +GG+HQM+GL E+ AAV RDSI LPE STN+TGASFETPCGRRLIKP
Sbjct: 69 EGPSPKTISSIGGLHQMVGLHEYGFAAVARDSIQCLPECGSTNKTGASFETPCGRRLIKP 128
Query: 185 VEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCIS 325
VEYM++I+SMKP++ +LADEVP+W ++KRN+ SVDRT++WLD+CI+
Sbjct: 129 VEYMEMITSMKPNLWATLADEVPAWANNKRNKTSVDRTVKWLDECIA 175
>ref|XP_004299874.1| PREDICTED: queuine tRNA-ribosyltransferase subunit QTRTD1-like
[Fragaria vesca subsp. vesca]
Length = 401
Score = 155 bits (391), Expect = 7e-36
Identities = 71/110 (64%), Positives = 92/110 (83%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+GIS TIS +GG+H+++GL +H A+PRDSI SLPE STN+ GASF+TP GR L+K
Sbjct: 68 LEGISPNTISKIGGLHKLVGLPDHGFVAIPRDSIQSLPECNSTNKLGASFDTPVGRSLVK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PVEY++LISS+KP+V +LADEVP+WV+ KRN+ SVDRT+RWLD+CI LN
Sbjct: 128 PVEYVELISSLKPNVWTALADEVPAWVNDKRNKTSVDRTVRWLDECIQLN 177
>ref|XP_002325954.2| hypothetical protein POPTR_0019s10480g [Populus trichocarpa]
gi|550317223|gb|EEF00336.2| hypothetical protein
POPTR_0019s10480g [Populus trichocarpa]
Length = 399
Score = 152 bits (384), Expect = 4e-35
Identities = 69/110 (62%), Positives = 93/110 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
++GIS+KTIS +GG+H+M+GL + LAAV RDSI LPE +TN+ GASFETPCGR LIK
Sbjct: 71 MEGISSKTISSIGGIHKMIGLHDFGLAAVARDSIQCLPESSATNKLGASFETPCGRLLIK 130
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P EYM +ISS++P++ +LADEVP+WVS KRN+ SVDRT++WLD+C++L+
Sbjct: 131 PAEYMGIISSLRPNLWATLADEVPAWVSKKRNKNSVDRTVKWLDECVALS 180
>gb|ESW05872.1| hypothetical protein PHAVU_011G216600g [Phaseolus vulgaris]
Length = 398
Score = 152 bits (383), Expect = 6e-35
Identities = 68/110 (61%), Positives = 92/110 (83%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+++ TIS +GG+HQMLGL ++ +AAVPRDSI LPE + + GASFETPCGR LIK
Sbjct: 70 LEGLASTTISKIGGLHQMLGLHQYGIAAVPRDSIQCLPESKGATKLGASFETPCGRLLIK 129
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +Y+++IS M+P++ +LADEVP+WVS KRN+ SVDRT+RWLDDC++LN
Sbjct: 130 PKDYVEMISCMRPNIWATLADEVPAWVSDKRNKTSVDRTVRWLDDCLALN 179
>ref|XP_004516911.1| PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like
[Cicer arietinum]
Length = 424
Score = 151 bits (381), Expect = 1e-34
Identities = 66/110 (60%), Positives = 93/110 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S TIS +GG+H+++GL ++ +AAVPRDSI S+PE +S ++ G +FETPCGR L+K
Sbjct: 73 LEGLSPTTISKIGGLHKLIGLPDYAIAAVPRDSIHSIPESKSASKLGPTFETPCGRLLVK 132
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +Y+++IS M+P++ +LADEVP+WVS KRNR SVDRTLRWLDDC++LN
Sbjct: 133 PKDYVQMISCMRPNIWATLADEVPAWVSDKRNRTSVDRTLRWLDDCLALN 182
>ref|XP_006836801.1| hypothetical protein AMTR_s00099p00022390 [Amborella trichopoda]
gi|548839365|gb|ERM99654.1| hypothetical protein
AMTR_s00099p00022390 [Amborella trichopoda]
Length = 397
Score = 148 bits (374), Expect = 6e-34
Identities = 69/106 (65%), Positives = 88/106 (83%)
Frame = +2
Query: 14 SAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIKPVEY 193
S KTISD+GGVH++LGL++ V RDSI+ LP E TN+ GASFETP GR L+KP +Y
Sbjct: 72 SEKTISDIGGVHELLGLQDCSFIGVARDSIVCLPHSEGTNKFGASFETPSGRLLVKPRDY 131
Query: 194 MKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
M++ISS+KP++ SLADEVPSWV+ KRNR+SVDRT+RWLDDCI+L+
Sbjct: 132 MEIISSLKPNLWTSLADEVPSWVTEKRNRSSVDRTIRWLDDCIALD 177
>gb|EXB81481.1| Queuine tRNA-ribosyltransferase subunit qtrtd1 [Morus notabilis]
Length = 367
Score = 148 bits (373), Expect = 8e-34
Identities = 69/110 (62%), Positives = 93/110 (84%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+G+S KTIS +GG+++MLGL ++ AAV RDS+ S+PEY STNR GASFET GR LIK
Sbjct: 68 LEGLSPKTISKIGGLNKMLGLPDYGFAAVARDSMQSIPEYSSTNRIGASFETRVGRLLIK 127
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
PV+Y+++IS M+P++ +LADEVP+WVS KRN+ SVDRT+RWLD+CI+L+
Sbjct: 128 PVDYVEMISCMRPNIWATLADEVPAWVSEKRNKTSVDRTVRWLDECIALS 177
>ref|XP_004983407.1| PREDICTED: queuine tRNA-ribosyltransferase subunit QTRTD1-like
[Setaria italica]
Length = 395
Score = 148 bits (373), Expect = 8e-34
Identities = 69/110 (62%), Positives = 90/110 (81%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
++G KTIS++GG+H+MLGL +HIL A DSI SLP E+TN+ GASF+TP GRRL+K
Sbjct: 67 IEGPPMKTISNIGGLHRMLGLPDHILVAAAGDSIESLPSSEATNKFGASFDTPSGRRLVK 126
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +YM+L+S MKP++ SLADEVP+WV+ KRN+ SVDRTLRWLD CI+L+
Sbjct: 127 PSDYMELVSCMKPNLWASLADEVPAWVTEKRNKTSVDRTLRWLDACIALD 176
>ref|XP_003605424.1| Queuine tRNA-ribosyltransferase [Medicago truncatula]
gi|355506479|gb|AES87621.1| Queuine
tRNA-ribosyltransferase [Medicago truncatula]
Length = 389
Score = 148 bits (373), Expect = 8e-34
Identities = 65/110 (59%), Positives = 91/110 (82%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
L+GIS TI +GG+H+++GL ++ + AVPRD++ SLPE + ++ GASFETPCGR LIK
Sbjct: 70 LEGISPATIGKIGGIHKLIGLGDYGVVAVPRDNVHSLPECKGASKLGASFETPCGRLLIK 129
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLN 331
P +Y+++IS M+P++ +LADEVP+WV+ KRNR SVDRTLRWLDDC++LN
Sbjct: 130 PKDYVEMISCMRPNIWATLADEVPAWVTDKRNRTSVDRTLRWLDDCLALN 179
>tpg|DAA46703.1| TPA: hypothetical protein ZEAMMB73_649296 [Zea mays]
Length = 398
Score = 147 bits (371), Expect = 1e-33
Identities = 71/111 (63%), Positives = 88/111 (79%)
Frame = +2
Query: 2 LDGISAKTISDVGGVHQMLGLREHILAAVPRDSIISLPEYESTNRTGASFETPCGRRLIK 181
++G KTIS + G+H MLGL +HIL A DSI SLP E+TN+ GASFETP GRRL+K
Sbjct: 70 IEGPPVKTISAIRGLHGMLGLPDHILVAAASDSIESLPSCEATNKFGASFETPSGRRLVK 129
Query: 182 PVEYMKLISSMKPDVSVSLADEVPSWVSSKRNRASVDRTLRWLDDCISLNV 334
P +YM+LIS MKP++ SLADEVP+WV+ KRN+ SVDRTLRWLD CI+L+V
Sbjct: 130 PSDYMELISCMKPNIWASLADEVPAWVTEKRNKVSVDRTLRWLDACIALDV 180