BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rauwolfia21_contig00009393
         (1340 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGX93059.1| 7-deoxyloganic acid 7-hydroxylase-like protein [R...   758   0.0  
gb|AGX93060.1| 7-deoxyloganic acid 7-hydroxylase-like protein [T...   704   0.0  
gb|AGX93062.1| CYP72A224 7-deoxyloganic acid 7-hydroxylase [Cath...   696   0.0  
gb|AGX93058.1| 7-deoxyloganic acid 7-hydroxylase-like protein [A...   696   0.0  
gb|AGX93061.1| 7-deoxyloganic acid 7-hydroxylase-like protein [V...   687   0.0  
gb|AGX93057.1| 7-deoxyloganic acid 7-hydroxylase-like protein [C...   664   0.0  
gb|AGX93056.1| 7-deoxyloganic acid 7-hydroxylase-like protein [L...   562   e-157
gb|ADU19849.1| putative secologanin synthase [Camptotheca acumin...   459   e-127
gb|AGX93046.1| secologanin synthase-like protein [Amsonia hubric...   429   e-117
gb|AGX93064.1| secologanin synthase [Catharanthus roseus]             423   e-116
ref|XP_004488668.1| PREDICTED: secologanin synthase-like [Cicer ...   422   e-115
gb|EXB52087.1| Secologanin synthase [Morus notabilis]                 421   e-115
ref|XP_006477948.1| PREDICTED: secologanin synthase-like [Citrus...   421   e-115
ref|XP_006477946.1| PREDICTED: secologanin synthase-like [Citrus...   421   e-115
gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]         421   e-115
gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]                           421   e-115
sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Shor...   420   e-115
ref|XP_004307203.1| PREDICTED: secologanin synthase-like [Fragar...   420   e-115
gb|AGX93044.1| secologanin synthase-like protein, partial [Lonic...   418   e-114
ref|XP_006377672.1| hypothetical protein POPTR_0011s101202g, par...   418   e-114

>gb|AGX93059.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Rauvolfia serpentina]
          Length = 520

 Score =  758 bits (1958), Expect = 0.0
 Identities = 372/372 (100%), Positives = 372/372 (100%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG
Sbjct: 1    MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
            KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK
Sbjct: 61   KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
            YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM
Sbjct: 121  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 180

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI
Sbjct: 181  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 240

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 1123
            RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN
Sbjct: 241  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 300

Query: 1124 LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 1303
            LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV
Sbjct: 301  LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 360

Query: 1304 FQVFGNKTPDYD 1339
            FQVFGNKTPDYD
Sbjct: 361  FQVFGNKTPDYD 372


>gb|AGX93060.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Tabernaemontana
            elegans]
          Length = 519

 Score =  704 bits (1816), Expect = 0.0
 Identities = 341/372 (91%), Positives = 357/372 (95%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            ME++FK V VLGF+GLALYWVYRVLDW+WF+PKKLEKCLREQGFKGNPYRLFLGDQYESG
Sbjct: 1    MEMNFKLVAVLGFIGLALYWVYRVLDWVWFKPKKLEKCLREQGFKGNPYRLFLGDQYESG 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
            KLI+EAMSKPIGVEEDVKKRI+PHILKTVETHGKNSFMWVGRIPRV +TDPELIKEVLTK
Sbjct: 61   KLIREAMSKPIGVEEDVKKRIIPHILKTVETHGKNSFMWVGRIPRVHVTDPELIKEVLTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
            YYKFQKNHHDLDPITKFLLTGIGSLEGE WAKRRKIINAAFHFEKLKLMLPAFYLSCRD+
Sbjct: 121  YYKFQKNHHDLDPITKFLLTGIGSLEGEPWAKRRKIINAAFHFEKLKLMLPAFYLSCRDL 180

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            V+KWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNY EGRRIFELMKELTALTIDVI
Sbjct: 181  VSKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYEEGRRIFELMKELTALTIDVI 240

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 1123
            RSVYIPG RFLPTKRNN+MRAIDKEVRVRI EII+ K KI+KAG+  A DDFLGILLECN
Sbjct: 241  RSVYIPGQRFLPTKRNNKMRAIDKEVRVRIKEIIDNKMKILKAGK-TAGDDFLGILLECN 299

Query: 1124 LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 1303
            LNEIREQG+NK AGMTIE+IIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQ RAREEV
Sbjct: 300  LNEIREQGNNKNAGMTIEQIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQNRAREEV 359

Query: 1304 FQVFGNKTPDYD 1339
             QVFGNKTPDYD
Sbjct: 360  LQVFGNKTPDYD 371


>gb|AGX93062.1| CYP72A224 7-deoxyloganic acid 7-hydroxylase [Catharanthus roseus]
          Length = 521

 Score =  696 bits (1796), Expect = 0.0
 Identities = 338/373 (90%), Positives = 356/373 (95%), Gaps = 1/373 (0%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            ME++FKS+  L FV L LYWVYR+LDW+WF+PKKLEKCLREQGFKGNPYRLFLGDQY+SG
Sbjct: 1    MELNFKSIIFLVFVSLTLYWVYRILDWVWFKPKKLEKCLREQGFKGNPYRLFLGDQYDSG 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
            KLI++A++KPIGVEEDVKKRIVPHILKTV THGK SFMWVGRIPRV ITDPELIKEVLTK
Sbjct: 61   KLIRQALTKPIGVEEDVKKRIVPHILKTVGTHGKKSFMWVGRIPRVNITDPELIKEVLTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
            YYKFQKNHHDLDPITK LLTGIGSLEG+ WAKRRKIINAAFHFEKLKLMLPAFYLSCRDM
Sbjct: 121  YYKFQKNHHDLDPITKLLLTGIGSLEGDPWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 180

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            V KWD KVPEGGSAEVDVWHDIETLTGDVISRTLFGSN+ EGRRIFELMKELTALTIDVI
Sbjct: 181  VTKWDNKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNFEEGRRIFELMKELTALTIDVI 240

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAA-AADDFLGILLEC 1120
            RSVYIPG RFLPTKRNNRMRAIDKEVRVRITEIINKK K+MK+GEAA AADDFLGILLEC
Sbjct: 241  RSVYIPGQRFLPTKRNNRMRAIDKEVRVRITEIINKKMKVMKSGEAASAADDFLGILLEC 300

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            NLNEI+EQG+NK+AGMTI EIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE
Sbjct: 301  NLNEIKEQGNNKSAGMTIGEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 360

Query: 1301 VFQVFGNKTPDYD 1339
            VFQVFGNKTPDYD
Sbjct: 361  VFQVFGNKTPDYD 373


>gb|AGX93058.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Amsonia hubrichtii]
          Length = 518

 Score =  696 bits (1796), Expect = 0.0
 Identities = 338/369 (91%), Positives = 353/369 (95%)
 Frame = +2

Query: 233  SFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKLI 412
            +FKSV VLGFV +ALYWVYR+LDW+WFRPKKLEKCLREQGFKGNPYRL LGDQYESGKLI
Sbjct: 3    NFKSVAVLGFVCVALYWVYRLLDWVWFRPKKLEKCLREQGFKGNPYRLLLGDQYESGKLI 62

Query: 413  KEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYYK 592
            +EAMSKPIG+EEDVKKRI+PH+LKTVETHGKNSFMWVGRIPRVQ+TDPELIKEVLTKYYK
Sbjct: 63   REAMSKPIGLEEDVKKRIIPHLLKTVETHGKNSFMWVGRIPRVQVTDPELIKEVLTKYYK 122

Query: 593  FQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVAK 772
            FQKNHHDLDPITK LLTGIGSLEGE WAKRRKIIN+AFHFEKLKLMLPAFYLSCRD+V+K
Sbjct: 123  FQKNHHDLDPITKLLLTGIGSLEGEPWAKRRKIINSAFHFEKLKLMLPAFYLSCRDLVSK 182

Query: 773  WDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRSV 952
            WDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNY EGRRIFELMKELTALTIDVIRSV
Sbjct: 183  WDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYEEGRRIFELMKELTALTIDVIRSV 242

Query: 953  YIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLNE 1132
            YIPG RFLPTKRNNRMR IDKEVRVRITEIIN K KIMK GE AA DDFLGILLECN NE
Sbjct: 243  YIPGQRFLPTKRNNRMRTIDKEVRVRITEIINNKMKIMKTGE-AAGDDFLGILLECNQNE 301

Query: 1133 IREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQV 1312
            IREQG+NK AGMTIE+IIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV QV
Sbjct: 302  IREQGNNKNAGMTIEQIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVLQV 361

Query: 1313 FGNKTPDYD 1339
            FG+KTPDYD
Sbjct: 362  FGDKTPDYD 370


>gb|AGX93061.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Vinca minor]
          Length = 520

 Score =  687 bits (1773), Expect = 0.0
 Identities = 333/370 (90%), Positives = 353/370 (95%)
 Frame = +2

Query: 230  VSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKL 409
            ++F SV +LGF+ L+++W+YRVLDW+WFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKL
Sbjct: 4    MNFNSVAILGFISLSIFWIYRVLDWVWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKL 63

Query: 410  IKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYY 589
            I+EAMSKPIG++EDVKKRIVPHILK VETHGKNSFMWVGRI RVQITDPELIKEVLTKYY
Sbjct: 64   IREAMSKPIGIDEDVKKRIVPHILKAVETHGKNSFMWVGRILRVQITDPELIKEVLTKYY 123

Query: 590  KFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVA 769
            KFQKNHHDLDPITK LL GIGSLEGE+WAKRRKIIN+AFHFEKLKLMLPAFYLSCRDMV 
Sbjct: 124  KFQKNHHDLDPITKLLLGGIGSLEGESWAKRRKIINSAFHFEKLKLMLPAFYLSCRDMVE 183

Query: 770  KWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRS 949
            KWDKKVPEGGS+EV+VWHDIETLTGDVISRTLFGSNY EGRRIFELMKELTALTIDVIRS
Sbjct: 184  KWDKKVPEGGSSEVEVWHDIETLTGDVISRTLFGSNYEEGRRIFELMKELTALTIDVIRS 243

Query: 950  VYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLN 1129
            VYIPG RFLPTKRNNRMRAIDKEVRVRITEIINKK KIMKAGE  AADDFLGILLE NLN
Sbjct: 244  VYIPGQRFLPTKRNNRMRAIDKEVRVRITEIINKKMKIMKAGE-TAADDFLGILLENNLN 302

Query: 1130 EIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQ 1309
            EIREQG+N+ AGMTIE+IIGECKLFYFAGQDTTSTL+VWTMVLLSRFPEWQTRAREEV Q
Sbjct: 303  EIREQGNNRNAGMTIEQIIGECKLFYFAGQDTTSTLIVWTMVLLSRFPEWQTRAREEVLQ 362

Query: 1310 VFGNKTPDYD 1339
            VFGNK PDYD
Sbjct: 363  VFGNKPPDYD 372


>gb|AGX93057.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Cinchona calisaya]
          Length = 519

 Score =  664 bits (1713), Expect = 0.0
 Identities = 316/372 (84%), Positives = 348/372 (93%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            ME++F S+ +LGF+GL++YW YRVLDW+W RPKKLEKCLREQGF+GNPYRLFLGDQYESG
Sbjct: 1    MELNFTSIAILGFIGLSIYWFYRVLDWVWLRPKKLEKCLREQGFRGNPYRLFLGDQYESG 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
            KLI+EAMSKPI V+EDV+KRI PHILKTV+THGKNSFMWVGRIPRV +TDPELI+EVLTK
Sbjct: 61   KLIREAMSKPIPVDEDVRKRIAPHILKTVQTHGKNSFMWVGRIPRVNVTDPELIREVLTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
            YYKFQKNHHDLDPITK LLTGIGSLEG+ W++RRKIIN+AF FEKLKLMLPAFYLSCRDM
Sbjct: 121  YYKFQKNHHDLDPITKLLLTGIGSLEGDPWSRRRKIINSAFQFEKLKLMLPAFYLSCRDM 180

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            V+KWD KVPE  SAE+DVWHDIETLTGDVISRTLFGSN+ EGRRIFELMKELT+LTIDVI
Sbjct: 181  VSKWDNKVPEDDSAELDVWHDIETLTGDVISRTLFGSNFEEGRRIFELMKELTSLTIDVI 240

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 1123
            RSVYIPG RFLPTKRNNRMRAID+EVRVRITEIINKK + MKAGE A  DDFLGILLECN
Sbjct: 241  RSVYIPGQRFLPTKRNNRMRAIDREVRVRITEIINKKMRAMKAGE-ATGDDFLGILLECN 299

Query: 1124 LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 1303
            LNEI+EQG+NK+AGM+IEEIIGECKLFYFAGQDTTSTL+VWTMVLLSRFPEWQ  AREEV
Sbjct: 300  LNEIKEQGNNKSAGMSIEEIIGECKLFYFAGQDTTSTLIVWTMVLLSRFPEWQQHAREEV 359

Query: 1304 FQVFGNKTPDYD 1339
             QVFG++ PDYD
Sbjct: 360  LQVFGDRKPDYD 371


>gb|AGX93056.1| 7-deoxyloganic acid 7-hydroxylase-like protein [Lonicera japonica]
          Length = 519

 Score =  562 bits (1448), Expect = e-157
 Identities = 269/359 (74%), Positives = 316/359 (88%), Gaps = 1/359 (0%)
 Frame = +2

Query: 266  GLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKLIKEAMSKPIGVE 445
            G+  YWVY  L+WIWFRPKKLEKCLREQGF GN YRLFLGDQ ES  +I++AMS+PI + 
Sbjct: 15   GVITYWVYSFLNWIWFRPKKLEKCLREQGFGGNAYRLFLGDQQESKVMIRDAMSRPITLS 74

Query: 446  EDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYYKFQKNHHDLDPI 625
            +D+K+R++PH+LKT+  HGKNSFMWVGR+PR+ IT+PELI++VLTKYYKFQKNHH LDPI
Sbjct: 75   DDIKQRVIPHVLKTMNNHGKNSFMWVGRMPRLHITEPELIRDVLTKYYKFQKNHHSLDPI 134

Query: 626  TKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVAKWDKKV-PEGGS 802
            TK+LL+GIGSLEGE WA+RR++IN+AFHFEKLKLMLPAFYLSC DMV KW+K V  +GGS
Sbjct: 135  TKYLLSGIGSLEGEPWAQRRRVINSAFHFEKLKLMLPAFYLSCLDMVNKWEKVVSSKGGS 194

Query: 803  AEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRSVYIPGHRFLPT 982
             EV+V HD+ETLTGDVISRTLFGSN+ EG++IFELMKELT LTI VI+SVYIPG RF+PT
Sbjct: 195  VEVEVHHDLETLTGDVISRTLFGSNFEEGKKIFELMKELTVLTIQVIQSVYIPGWRFMPT 254

Query: 983  KRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLNEIREQGHNKTA 1162
            KRNNR++ IDK+VRV ITEIIN K K MKAGE++++ DFLGILLECN+ EI EQ  NK A
Sbjct: 255  KRNNRIKKIDKDVRVSITEIINNKMKAMKAGESSSS-DFLGILLECNMTEI-EQTKNKNA 312

Query: 1163 GMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQVFGNKTPDYD 1339
            G++IEEIIGECKLFYFAGQDTTSTLL WTMV+LSRFP+WQ RAREEV QVFG+  PDYD
Sbjct: 313  GLSIEEIIGECKLFYFAGQDTTSTLLCWTMVILSRFPDWQARAREEVLQVFGDGKPDYD 371


>gb|ADU19849.1| putative secologanin synthase [Camptotheca acuminata]
          Length = 524

 Score =  459 bits (1182), Expect = e-127
 Identities = 216/373 (57%), Positives = 284/373 (76%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            +M+V +KS+     V   +Y  +++L+W +  PK++EKCLR+QGFKGN YRL +GD  ES
Sbjct: 4    QMDVLYKSIAASVAVVFLVY-AWKMLNWAYLTPKRIEKCLRKQGFKGNSYRLLVGDLKES 62

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
              ++KE MSKPI V ED+ +R++PH++KT++T+GKNSF W+GR+PRV I +P+LIK++L 
Sbjct: 63   SMMLKETMSKPINVSEDIVERVMPHVIKTIDTYGKNSFTWIGRMPRVHIMEPDLIKDILA 122

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
             +  F KNHH  +P+TKFLLTGIGSLEG+ WAK R+II+ +FH EKLK MLPAFY+S  D
Sbjct: 123  NHNDFMKNHHAYNPLTKFLLTGIGSLEGDKWAKHRRIISPSFHLEKLKTMLPAFYVSYDD 182

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            ++ KW+++    GS E+D++   +TLT DVISR  FGS+YGEG RIF L+KEL  LT+DV
Sbjct: 183  LLTKWEQQCSSKGSVEIDLFPTFDTLTSDVISRVAFGSSYGEGGRIFILLKELMDLTVDV 242

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +RSVY+PG  FLPTKRNNRMR +D E++ R++ IIN + K MKAGE  + +D LG LLE 
Sbjct: 243  MRSVYVPGSSFLPTKRNNRMREVDGEIKDRLSGIINSRVKAMKAGE-PSGEDLLGTLLES 301

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N  EI   G+ K AGM+IE++I ECKLFYFAGQ+TT  LL WT VLLSR PEWQ RAREE
Sbjct: 302  NFKEIERLGNKKNAGMSIEDVISECKLFYFAGQETTGILLTWTCVLLSRHPEWQERAREE 361

Query: 1301 VFQVFGNKTPDYD 1339
            +FQVFGN   D+D
Sbjct: 362  IFQVFGNGKVDFD 374


>gb|AGX93046.1| secologanin synthase-like protein [Amsonia hubrichtii]
          Length = 524

 Score =  429 bits (1103), Expect = e-117
 Identities = 207/373 (55%), Positives = 269/373 (72%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            +M++  K++    F  L L W +RVLDW WF PK+LEK LR+QG+KGN YR  +GD  E+
Sbjct: 4    DMDIIRKAIAASVFA-LVLAWAWRVLDWAWFTPKRLEKRLRQQGYKGNAYRFLVGDVKEN 62

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
            GK+++EAMSKP+    D+  RI+PHI  T++T+G+NSF W+GRIPR+ + +PELIKEVLT
Sbjct: 63   GKMLQEAMSKPMAFNNDIVPRIMPHIHHTIQTYGRNSFTWMGRIPRIHVMEPELIKEVLT 122

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
               K+QKN    +P+ KFLLTG+GS EG  W+K R+II+ AF  EKLK MLPAF +S  D
Sbjct: 123  HSSKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKTMLPAFAISYDD 182

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            M+ KW+K   + GS EVD++   + LT DVIS+  FGS Y EG +IF+L+KEL  LTI+ 
Sbjct: 183  MLRKWEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFQLLKELMDLTIEC 242

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +R VYIPG  +LPTKRN RMR I+KE+   +  IINK+TK +KAGE    DD LG+LLE 
Sbjct: 243  MRDVYIPGWSYLPTKRNKRMREINKELTDMLRNIINKRTKALKAGE-PGEDDLLGVLLES 301

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N  EI++QG+ K  GMTI ++I ECKLFYFAGQ+TT  LL W  +LLS+ PEWQ RAREE
Sbjct: 302  NFQEIQKQGNKKNVGMTINDVIEECKLFYFAGQETTGVLLTWATILLSKHPEWQERAREE 361

Query: 1301 VFQVFGNKTPDYD 1339
            V Q FG   PD++
Sbjct: 362  VLQAFGKNKPDFE 374


>gb|AGX93064.1| secologanin synthase [Catharanthus roseus]
          Length = 527

 Score =  423 bits (1087), Expect = e-116
 Identities = 202/373 (54%), Positives = 268/373 (71%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            +M++  K++    F  L + W +RVLDW WF PK++EK LR+QGF+GNPYR  +GD  ES
Sbjct: 4    DMDIIRKAIAATIFA-LVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKES 62

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
            GK+ +EA+S P+  + D+  R++PHI  T++T+G+NSF W+GRIPR+ + +PELIKEVLT
Sbjct: 63   GKMHQEALSNPMEFDNDIVPRLMPHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLT 122

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
               K+QKN    +P+ KFLLTG+GS EG  W+K R+II+ AF  EKLK MLPAF +   D
Sbjct: 123  HSSKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHD 182

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            M+ KW+K   + GS EVD++   + LT DVIS+  FGS Y EG +IF L+KEL  LTID 
Sbjct: 183  MLTKWEKLAEKEGSHEVDIFPTFDVLTSDVISKVAFGSTYDEGGKIFRLLKELMDLTIDC 242

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +R VYIPG  +LPTKRN RM+ I+KE+   +  IINK+ K +KAGE    DD LG+LLE 
Sbjct: 243  MRDVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLES 301

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N+ EI++QG+ K  GMTI ++I ECKLFYFAGQ+TT  LL WT +LLS+ PEWQ RAREE
Sbjct: 302  NIQEIQKQGNRKDGGMTINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREE 361

Query: 1301 VFQVFGNKTPDYD 1339
            V Q FG   P+++
Sbjct: 362  VLQAFGKNKPEFE 374


>ref|XP_004488668.1| PREDICTED: secologanin synthase-like [Cicer arietinum]
          Length = 519

 Score =  422 bits (1084), Expect = e-115
 Identities = 202/357 (56%), Positives = 263/357 (73%)
 Frame = +2

Query: 269  LALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKLIKEAMSKPIGVEE 448
            L+L W +R+L+W+W +PKKLEK LREQGFKGN YRL +GD  +  K  KEA SKP+ + +
Sbjct: 16   LSLTWAWRMLNWLWLKPKKLEKLLREQGFKGNSYRLLVGDVRDLLKTRKEATSKPMNLSD 75

Query: 449  DVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYYKFQKNHHDLDPIT 628
            D+  R+  ++  +V  +GKNSFMW G IPRV ITDPE IK+VL K Y F+K + +  P+ 
Sbjct: 76   DIGPRVFSYVHHSVTKYGKNSFMWFGPIPRVNITDPEQIKDVLNKIYDFRKPNSN--PLV 133

Query: 629  KFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVAKWDKKVPEGGSAE 808
            + L  G+ S EGE W K RKIIN AFH EKLK+MLP F+ SC D+++KW+K +   GS E
Sbjct: 134  RLLANGLVSYEGEKWNKHRKIINPAFHLEKLKIMLPIFFKSCDDLISKWEKMLSLEGSCE 193

Query: 809  VDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRSVYIPGHRFLPTKR 988
            +DVW  ++ L  DVISRT FGS++ EG+RIF+L  E   LT  ++  VYIPG RFLPT  
Sbjct: 194  MDVWPFLQNLASDVISRTAFGSSFEEGKRIFQLQIEQAELTKQIMMKVYIPGWRFLPTTT 253

Query: 989  NNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLNEIREQGHNKTAGM 1168
            + RM+ ID++++  +T++INK+ K +KAGE A  DD LGILLE N  E+ E G+NK  GM
Sbjct: 254  HKRMKEIDRDIKASLTDMINKREKALKAGE-ATKDDLLGILLESNHREMEEHGNNKNVGM 312

Query: 1169 TIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQVFGNKTPDYD 1339
            ++E++I ECKLFYFAGQ+TTS LLVWTMV LSR+P+WQ RAREEVFQVFGNK PD+D
Sbjct: 313  SLEDVIEECKLFYFAGQETTSVLLVWTMVCLSRYPDWQARAREEVFQVFGNKKPDFD 369


>gb|EXB52087.1| Secologanin synthase [Morus notabilis]
          Length = 518

 Score =  421 bits (1083), Expect = e-115
 Identities = 209/373 (56%), Positives = 265/373 (71%), Gaps = 1/373 (0%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALY-WVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            MEVSF  ++ + F  L +  W +RVL+W+W RPK+LE CLR+QG KGN YRL+ GD  ES
Sbjct: 1    MEVSFLKLSSVVFAILLITTWAWRVLNWVWLRPKRLENCLRKQGLKGNSYRLWSGDLKES 60

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
              +IKEA SKPI + ED+  RI P  L TV  +GK+SFMW+G  PRV IT+PE IK++  
Sbjct: 61   SLMIKEASSKPINLSEDITARIFPFGLHTVNNYGKDSFMWIGPTPRVSITNPEYIKDIFN 120

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
            K   F K      P+ K L TG+ + EGE W + RKIIN AFH EKLK ++PAFYLSC +
Sbjct: 121  KLGDFPKPQSI--PLVKLLATGVATYEGEKWTRHRKIINPAFHMEKLKRLVPAFYLSCSE 178

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            M+ KW++ +   GS E+DVW  ++ +TGDVISRT FGS+Y EGRRIF+L KE   L I  
Sbjct: 179  MLNKWERLLNSEGSCELDVWSYLQNMTGDVISRTAFGSSYEEGRRIFQLQKEQGELAIKS 238

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +++VYIPG RF+PTK N RM+ I  E+   +  II+K+   M+AGE A  DD LGIL+E 
Sbjct: 239  LQTVYIPGWRFVPTKLNRRMKKIYNEIEALVKNIIHKREGAMRAGE-APNDDLLGILMES 297

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            NL EI+E G+NK  G++I ++I ECKLFY AGQ+TTSTLLVWTMVLLSRFP WQ RAREE
Sbjct: 298  NLKEIQEHGNNKNMGISIRDVIEECKLFYIAGQETTSTLLVWTMVLLSRFPSWQDRAREE 357

Query: 1301 VFQVFGNKTPDYD 1339
            V Q+FGN  P +D
Sbjct: 358  VLQIFGNNKPGFD 370


>ref|XP_006477948.1| PREDICTED: secologanin synthase-like [Citrus sinensis]
          Length = 522

 Score =  421 bits (1083), Expect = e-115
 Identities = 206/372 (55%), Positives = 266/372 (71%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            ME+  KS+ +   +   L W +RVL+W+W RP+KLEK LR+QG KGN YRL  GD  E+ 
Sbjct: 1    MELPLKSIALTIVIVTVLTWAWRVLNWVWLRPEKLEKFLRQQGLKGNSYRLLFGDLKENS 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
              +KEA ++P+ +++D+  R+ P + K V  +GKNSFMW G  PRV I +P+ IK + TK
Sbjct: 61   IELKEAKARPLSLDDDIAIRVNPFLHKLVNDYGKNSFMWFGPTPRVNIMNPDQIKAIFTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
               FQK +    P+ + L+ G+ +LEGE WAK RK+IN AFH EKLKLMLPAFYLSC ++
Sbjct: 121  INDFQKVNSI--PLARLLIVGLATLEGEKWAKHRKLINPAFHQEKLKLMLPAFYLSCIEI 178

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            + KW+K++   GS+E+DVW  +  LT DVISRT FGS+Y EGRRIF+L  EL  LT+ V 
Sbjct: 179  ITKWEKQMSVEGSSELDVWPYLANLTSDVISRTAFGSSYEEGRRIFQLQAELAELTMQVF 238

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 1123
            RSV+IPG RFLPTKRN RM+ IDKE+R  +  II  + K M+AGE AA DD LGIL+E +
Sbjct: 239  RSVHIPGWRFLPTKRNRRMKEIDKEIRASLMGIIKNREKAMRAGE-AANDDLLGILMETS 297

Query: 1124 LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 1303
              EI E G+NK  G ++ ++I ECKLFYFAGQ+TTS LL WTMVLLS+  +WQ RAR+EV
Sbjct: 298  FREIEEHGNNKNVGFSMNDVIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEV 357

Query: 1304 FQVFGNKTPDYD 1339
             QVFGN  PDYD
Sbjct: 358  LQVFGNNKPDYD 369


>ref|XP_006477946.1| PREDICTED: secologanin synthase-like [Citrus sinensis]
          Length = 522

 Score =  421 bits (1082), Expect = e-115
 Identities = 206/372 (55%), Positives = 266/372 (71%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            ME+  KS+ +   +   L W +RVL+W+W RPKKLEK LR+QG KGN YRL  GD  E+ 
Sbjct: 1    MELPLKSIALTIVIVTVLTWAWRVLNWVWLRPKKLEKFLRQQGLKGNSYRLLFGDLKENS 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
              +KEA ++P+ +++D+  R+ P + K V  +GKNSFMW G  PRV I +P+ IK + TK
Sbjct: 61   IELKEAKARPLSLDDDIAIRVNPFLHKLVNDYGKNSFMWFGPTPRVNIMNPDQIKAIFTK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
               FQK +    P+ + L+ G+ +LEGE WAK RK+IN AFH EKLKLMLPAFYLSC ++
Sbjct: 121  INDFQKVNSI--PLARLLIVGLATLEGEKWAKHRKLINPAFHQEKLKLMLPAFYLSCIEI 178

Query: 764  VAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVI 943
            + KW+K++   GS+E+DVW  +  LT DVISRT FGS+Y EGRRIF+L  EL  LT+ V 
Sbjct: 179  ITKWEKQMSVEGSSELDVWPYLANLTSDVISRTAFGSSYEEGRRIFQLQAELAELTMQVF 238

Query: 944  RSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECN 1123
            RSV+IPG RFLPTKRN RM+ IDKE+R  +  II  + K M+AGE AA +D LGIL+E +
Sbjct: 239  RSVHIPGWRFLPTKRNRRMKEIDKEIRASLMGIIKNREKAMRAGE-AANNDLLGILMETS 297

Query: 1124 LNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEV 1303
              EI E G+NK  G ++ ++I ECKLFYFAGQ+TTS LL WTMVLLS+  +WQ RAR+EV
Sbjct: 298  FREIEEHGNNKNVGFSMNDVIEECKLFYFAGQETTSVLLNWTMVLLSKHQDWQERARQEV 357

Query: 1304 FQVFGNKTPDYD 1339
             QVFGN  PDYD
Sbjct: 358  LQVFGNNKPDYD 369


>gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]
          Length = 516

 Score =  421 bits (1082), Expect = e-115
 Identities = 199/357 (55%), Positives = 260/357 (72%)
 Frame = +2

Query: 269  LALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKLIKEAMSKPIGVEE 448
            L + W +RVLDW WF PK++EK LR+QGF+GNPYR  +GD  ESGK+ +EA+S P+  + 
Sbjct: 8    LVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALSNPMEFDN 67

Query: 449  DVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYYKFQKNHHDLDPIT 628
            D+  R++PHI  T++T+G+NSF W+GRIPR+ + +PELIKEVLT   K+QKN    +P+ 
Sbjct: 68   DIVPRLMPHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLV 127

Query: 629  KFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVAKWDKKVPEGGSAE 808
            KFLLTG+GS EG  W+K R+II+ AF  EKLK MLPAF +   DM+ KW+K   + GS E
Sbjct: 128  KFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKLAEKEGSHE 187

Query: 809  VDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRSVYIPGHRFLPTKR 988
            VD++   + LT DVIS+  FGS Y EG +IF L+KEL  LTID +R VYIPG  +LPTKR
Sbjct: 188  VDIFPTFDVLTSDVISKVAFGSTYDEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKR 247

Query: 989  NNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLNEIREQGHNKTAGM 1168
            N RM+ I+KE+   +  IINK+ K +KAGE    DD LG+LLE N+ EI++QG+ K  GM
Sbjct: 248  NKRMKEINKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLESNIQEIQKQGNRKDGGM 306

Query: 1169 TIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQVFGNKTPDYD 1339
            TI ++I ECKLFYFAGQ+TT  LL WT +LLS+ PEWQ RAREEV Q FG   P+++
Sbjct: 307  TINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKPEFE 363


>gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]
          Length = 518

 Score =  421 bits (1081), Expect = e-115
 Identities = 208/352 (59%), Positives = 263/352 (74%)
 Frame = +2

Query: 284  VYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESGKLIKEAMSKPIGVEEDVKKR 463
            ++RVL+W+WFRPKKLEK LR+QG KGN Y++  GD  E   +IKEA SKP+ + +D+  R
Sbjct: 23   IWRVLNWVWFRPKKLEKLLRKQGLKGNSYKILYGDMKELSGMIKEANSKPMNLSDDIAPR 82

Query: 464  IVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTKYYKFQKNHHDLDPITKFLLT 643
            +VP  L T++ +GK SF+W+G  P V I DPELIKE+ +KYY +QK H +  P+TK L+ 
Sbjct: 83   LVPFFLDTIKKYGKKSFVWLGPKPLVLIMDPELIKEIFSKYYLYQKPHGN--PVTKLLVQ 140

Query: 644  GIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDMVAKWDKKVPEGGSAEVDVWH 823
            G+ SLE + WAK RKIIN AFH EKLK MLPAF LSC +M+ KW+  V   GS E+DVW 
Sbjct: 141  GLVSLEEDKWAKHRKIINPAFHLEKLKHMLPAFCLSCTEMLCKWEDIVSIKGSHEIDVWP 200

Query: 824  DIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDVIRSVYIPGHRFLPTKRNNRMR 1003
             +E L+ DVISRT FGSN+ EG+RIFEL KE     ++ IRSVYIPG RFLPTKRN RM+
Sbjct: 201  HLEQLSSDVISRTAFGSNFEEGKRIFELQKEQAQYFVEAIRSVYIPGWRFLPTKRNRRMK 260

Query: 1004 AIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLECNLNEIREQGHNKTAGMTIEEI 1183
             ++K+VR  I  II+K+ K MKAGE A+ +D LGILLE N  E  EQ  +K + M+IEE+
Sbjct: 261  EVEKDVRASIRGIIDKRVKAMKAGE-ASNEDLLGILLESNFTE-AEQHRHKDSAMSIEEV 318

Query: 1184 IGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREEVFQVFGNKTPDYD 1339
            I ECKLFY AGQ+TTS LLVWT++LLSR  +WQ+RAREEVFQVFGN+ PD+D
Sbjct: 319  IQECKLFYVAGQETTSVLLVWTLILLSRHQDWQSRAREEVFQVFGNQKPDFD 370


>sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Short=SLS; AltName:
            Full=CYPLXXII; AltName: Full=Cytochrome P450 72A1
            gi|167484|gb|AAA33106.1| cytochrome P-450 protein
            [Catharanthus roseus] gi|445604|prf||1909351A cytochrome
            P450
          Length = 524

 Score =  420 bits (1080), Expect = e-115
 Identities = 202/373 (54%), Positives = 266/373 (71%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            +M+   K++    F  L + W +RVLDW WF PK++EK LR+QGF+GNPYR  +GD  ES
Sbjct: 4    DMDTIRKAIAATIFA-LVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKES 62

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
            GK+ +EA+SKP+    D+  R++PHI  T+ T+G+NSF W+GRIPR+ + +PELIKEVLT
Sbjct: 63   GKMHQEALSKPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLT 122

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
               K+QKN    +P+ KFLLTG+GS EG  W+K R+II+ AF  EKLK MLPAF +   D
Sbjct: 123  HSSKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHD 182

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            M+ KW+K   + GS EVD++   + LT DVIS+  FGS Y EG +IF L+KEL  LTID 
Sbjct: 183  MLTKWEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDC 242

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +R VYIPG  +LPTKRN RM+ I+KE+   +  IINK+ K +KAGE    DD LG+LLE 
Sbjct: 243  MRDVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGE-PGEDDLLGVLLES 301

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N+ EI++QG+ K  GM+I ++I ECKLFYFAGQ+TT  LL WT +LLS+ PEWQ RAREE
Sbjct: 302  NIQEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREE 361

Query: 1301 VFQVFGNKTPDYD 1339
            V Q FG   P+++
Sbjct: 362  VLQAFGKNKPEFE 374


>ref|XP_004307203.1| PREDICTED: secologanin synthase-like [Fragaria vesca subsp. vesca]
          Length = 543

 Score =  420 bits (1080), Expect = e-115
 Identities = 202/373 (54%), Positives = 262/373 (70%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            +ME+SF     +  + +A    +RVL+W+W +PKKLEKCLR+QG  GN YR+ +GD  ES
Sbjct: 25   QMELSFIIALSIVLLSIASSCAWRVLNWVWLKPKKLEKCLRQQGLAGNSYRVLIGDVKES 84

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
             +++KEA SKP+ + +D   R++P + +T+  HGKNSF W G  PRV I +P+ + E+LT
Sbjct: 85   FRVMKEACSKPMNLSDDAAPRVLPFLHQTITKHGKNSFTWFGTTPRVTIMNPDYLNEILT 144

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
            KY  F++ H    P+ K L  G+ +LEGE W K+RKIIN AFH EKLK MLPA Y SC D
Sbjct: 145  KYDDFRRPH--TAPVVKLLSYGLANLEGEKWVKQRKIINPAFHIEKLKNMLPAIYQSCTD 202

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            M+ KW+  V + GS E+D+W  ++ L+GDVISRT FGS+Y EGR IFEL KE T L I  
Sbjct: 203  MITKWEIMVSKEGSCELDIWPFLQNLSGDVISRTAFGSSYEEGRTIFELQKEQTELLIKA 262

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            + S YIPG RFLPTK N RM+AI KEV   +  II+ K + ++AG+  A +D LGILLE 
Sbjct: 263  LFSAYIPGFRFLPTKLNRRMKAISKEVEATMKRIISNKVEAIRAGDEDAKNDLLGILLES 322

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N  EIR+ G NK  GM+IEE+IGECKLFY AG +TTS LL WTM+LL R+P WQ RAR+E
Sbjct: 323  NWKEIRQYGGNKNLGMSIEEVIGECKLFYLAGAETTSILLTWTMILLCRYPNWQARARQE 382

Query: 1301 VFQVFGNKTPDYD 1339
            V QVFG+  PD+D
Sbjct: 383  VLQVFGSNKPDFD 395


>gb|AGX93044.1| secologanin synthase-like protein, partial [Lonicera japonica]
          Length = 520

 Score =  418 bits (1075), Expect = e-114
 Identities = 207/373 (55%), Positives = 268/373 (71%)
 Frame = +2

Query: 221  EMEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYES 400
            E+E+  K++ V    G+ L W +RVL+W WF PKKLEK LR+QGFKGNPYR  +GD  ES
Sbjct: 2    EVELIHKTIGVSILFGILL-WAWRVLNWAWFTPKKLEKKLRDQGFKGNPYRFLVGDVKES 60

Query: 401  GKLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLT 580
              ++++AMSKPI    D+  RI+PHI  T++T G NSF W+GRIPR+ I DPELI+EVLT
Sbjct: 61   AAMLEQAMSKPIPYSNDIVPRIMPHIDHTIKTFGDNSFTWMGRIPRIHIMDPELIREVLT 120

Query: 581  KYYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRD 760
               KF KN    +P+ KFLLTG+GS EG+ W   R+II+ AF  +KLK MLPAF +S  +
Sbjct: 121  HSTKFVKNFDVHNPLAKFLLTGVGSFEGDKWRMHRRIISPAFTLDKLKGMLPAFAISYDE 180

Query: 761  MVAKWDKKVPEGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            +++ W+      GS EVDV+   +TLT DVIS+  FGSNY EG +IF+L+KEL  LTI V
Sbjct: 181  LLSHWETVAHNYGSHEVDVFPTFDTLTSDVISKVAFGSNYDEGGKIFQLLKELMDLTIMV 240

Query: 941  IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLEC 1120
            +R VYIPG  +LPTK NNRM+ I+ E++  + +IINK+ K MKAGE A+ DD LG+LL+ 
Sbjct: 241  MRDVYIPGWSYLPTKTNNRMKEINNELKSMLRKIINKRVKAMKAGE-ASEDDLLGVLLDS 299

Query: 1121 NLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRAREE 1300
            N  EI+  G+ K AGMTI+++I ECKLFYFAGQ+TT  LL WTMV+LS+ PEWQ RAREE
Sbjct: 300  NFKEIQRLGNKKNAGMTIDDVIEECKLFYFAGQETTGILLTWTMVMLSKHPEWQDRAREE 359

Query: 1301 VFQVFGNKTPDYD 1339
            V   FG   PD++
Sbjct: 360  VISAFGKSKPDFE 372


>ref|XP_006377672.1| hypothetical protein POPTR_0011s101202g, partial [Populus
            trichocarpa] gi|550328052|gb|ERP55469.1| hypothetical
            protein POPTR_0011s101202g, partial [Populus trichocarpa]
          Length = 396

 Score =  418 bits (1075), Expect = e-114
 Identities = 206/374 (55%), Positives = 271/374 (72%), Gaps = 2/374 (0%)
 Frame = +2

Query: 224  MEVSFKSVTVLGFVGLALYWVYRVLDWIWFRPKKLEKCLREQGFKGNPYRLFLGDQYESG 403
            M V+  S+ V       L W +RVL+W+WFRPKK+E+CLR+QGF G PYRL  GD  E+ 
Sbjct: 1    MTVTVTSILVSIVYVAVLRWAWRVLNWVWFRPKKVERCLRQQGFAGKPYRLLFGDWKENS 60

Query: 404  KLIKEAMSKPIGVEEDVKKRIVPHILKTVETHGKNSFMWVGRIPRVQITDPELIKEVLTK 583
             ++KEA +KPIG+ + +  R++P + + V+ +GKNSFMW+G  PRV I +P+ I++V  K
Sbjct: 61   DMLKEARTKPIGLSDALLPRVMPFLHQLVKDYGKNSFMWIGPKPRVNIMNPDQIRDVFMK 120

Query: 584  YYKFQKNHHDLDPITKFLLTGIGSLEGETWAKRRKIINAAFHFEKLKLMLPAFYLSCRDM 763
              ++QK  H   P+ K L  G+ S EGE WAK RKIIN AFH EKLKLM+PAFY SC  M
Sbjct: 121  INEYQKPSH---PLLKPLACGLASHEGEKWAKHRKIINPAFHQEKLKLMIPAFYESCSGM 177

Query: 764  VAKWDKKVP-EGGSAEVDVWHDIETLTGDVISRTLFGSNYGEGRRIFELMKELTALTIDV 940
            + KW+K V  + GS E+DVW D++ LT D ISRT FGSNY EG+RIF+L+KELT LT+ V
Sbjct: 178  INKWEKLVSVDEGSCELDVWPDLQGLTCDAISRTSFGSNYEEGKRIFDLLKELTDLTVHV 237

Query: 941  -IRSVYIPGHRFLPTKRNNRMRAIDKEVRVRITEIINKKTKIMKAGEAAAADDFLGILLE 1117
             I++  IPG+RFLP   N R++AIDKE++  +  +INK+ K M AGE  A +D LG+LLE
Sbjct: 238  IIKAFVIPGYRFLPIPTNRRLKAIDKEIKASLNALINKREKAMSAGE-DAKNDLLGLLLE 296

Query: 1118 CNLNEIREQGHNKTAGMTIEEIIGECKLFYFAGQDTTSTLLVWTMVLLSRFPEWQTRARE 1297
             N  EI+E G+ K+  M+IE++I ECK+FYFAGQ+TTS LL WTMVLL+++P WQ RARE
Sbjct: 297  SNFREIQEHGNTKSVRMSIEDVIDECKIFYFAGQETTSVLLTWTMVLLAQYPNWQARARE 356

Query: 1298 EVFQVFGNKTPDYD 1339
            EV QVFGNK PD+D
Sbjct: 357  EVVQVFGNKKPDFD 370