BLASTX nr result
ID: Rauwolfia21_contig00004077
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rauwolfia21_contig00004077
(376 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO13736.1|AF253415_1 minovincinine 19-hydroxy-O-acetyltransf... 107 2e-21
sp|Q9ZTK5.1|DAT_CATRO RecName: Full=Deacetylvindoline O-acetyltr... 103 2e-20
dbj|BAB01067.1| acetyltranferase-like protein [Arabidopsis thali... 73 3e-11
ref|NP_189233.1| HXXXD-type acyl-transferase-like protein [Arabi... 73 3e-11
ref|XP_006363208.1| PREDICTED: deacetylvindoline O-acetyltransfe... 71 2e-10
ref|XP_004232634.1| PREDICTED: deacetylvindoline O-acetyltransfe... 70 2e-10
ref|XP_004232635.1| PREDICTED: deacetylvindoline O-acetyltransfe... 70 3e-10
ref|XP_002267080.1| PREDICTED: vinorine synthase-like [Vitis vin... 70 4e-10
emb|CAN63243.1| hypothetical protein VITISV_028808 [Vitis vinifera] 70 4e-10
emb|CAN69388.1| hypothetical protein VITISV_027664 [Vitis vinifera] 70 4e-10
ref|XP_006351214.1| PREDICTED: deacetylvindoline O-acetyltransfe... 69 8e-10
ref|XP_004140566.1| PREDICTED: BAHD acyltransferase At5g47980-li... 69 8e-10
ref|XP_002875299.1| transferase family protein [Arabidopsis lyra... 68 1e-09
ref|XP_006292162.1| hypothetical protein CARUB_v10018368mg [Caps... 67 2e-09
ref|XP_006470642.1| PREDICTED: BAHD acyltransferase At5g47980-li... 67 2e-09
ref|XP_006446155.1| hypothetical protein CICLE_v10015146mg [Citr... 67 2e-09
ref|XP_006362683.1| PREDICTED: deacetylvindoline O-acetyltransfe... 66 4e-09
ref|XP_006344158.1| PREDICTED: deacetylvindoline O-acetyltransfe... 66 4e-09
ref|XP_006471129.1| PREDICTED: BAHD acyltransferase At5g47980-li... 66 5e-09
ref|XP_004295692.1| PREDICTED: BAHD acyltransferase At5g47980-li... 66 5e-09
>gb|AAO13736.1|AF253415_1 minovincinine 19-hydroxy-O-acetyltransferase [Catharanthus roseus]
Length = 443
Score = 107 bits (267), Expect = 2e-21
Identities = 60/133 (45%), Positives = 91/133 (68%), Gaps = 10/133 (7%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRS----TNCGSSRPSVLLQPMNIRSL 173
IE+LKSKA++ GI+KPTRV+V+TA L + A VA +S NCG S P ++Q +N+R L
Sbjct: 225 IERLKSKAIEFGIEKPTRVEVLTAFLCRCATVAGKSAAKNNNCGQSLPFAVIQAVNLRPL 284
Query: 174 IKLPQNAIGNIFSFNFCVNEDEG-----MDFSALV-GQLRKAKMKSKDLSEDELTYESQM 335
++LP+N++GN+ S F ++ +F+ LV G+LRKAK K K+LS+++L Y ++M
Sbjct: 285 LELPKNSVGNLISIYFSTIKENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVARM 344
Query: 336 QVLELCLKQHNIS 374
Q CLK+ +IS
Sbjct: 345 QDFANCLKELDIS 357
>sp|Q9ZTK5.1|DAT_CATRO RecName: Full=Deacetylvindoline O-acetyltransferase; AltName:
Full=Acetyl-coenzyme A:deacetylvindoline
4-O-acetyltransferase gi|4091808|gb|AAC99311.1|
deacetylvindoline 4-O-acetyltransferase [Catharanthus
roseus]
Length = 439
Score = 103 bits (258), Expect = 2e-20
Identities = 59/133 (44%), Positives = 90/133 (67%), Gaps = 10/133 (7%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRS----TNCGSSRPSVLLQPMNIRSL 173
IEKLKSKAV+ GI+KPTRV+V+TA L + A VA +S NCG S P +LQ +N+R +
Sbjct: 226 IEKLKSKAVEFGIEKPTRVEVLTAFLSRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPI 285
Query: 174 IKLPQNAIGNIFS--FNFCVNEDEGMD----FSALVGQLRKAKMKSKDLSEDELTYESQM 335
++LPQN++GN+ S F+ + E++ ++ ++ +LRK K K K+LS ++LTY +QM
Sbjct: 286 LELPQNSVGNLVSIYFSRTIKENDYLNEKEYTKLVINELRKEKQKIKNLSREKLTYVAQM 345
Query: 336 QVLELCLKQHNIS 374
+ LK+ +IS
Sbjct: 346 EEFVKSLKEFDIS 358
>dbj|BAB01067.1| acetyltranferase-like protein [Arabidopsis thaliana]
Length = 455
Score = 73.2 bits (178), Expect = 3e-11
Identities = 41/104 (39%), Positives = 71/104 (68%), Gaps = 5/104 (4%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLP 185
I+ L++KA + +PTRV+ V+AL++KSAM ATR+ + G+S+PS+L +++RS + P
Sbjct: 237 IQALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVS-GTSKPSILANSVSLRSRVSPP 295
Query: 186 --QNAIGNIFSFNFCVNEDEGMD---FSALVGQLRKAKMKSKDL 302
+N+IGN+ S+ F +EG++ LV ++RKAK + +D+
Sbjct: 296 FTKNSIGNLVSY-FAAKAEEGINQTKLQTLVSKIRKAKQRFRDI 338
>ref|NP_189233.1| HXXXD-type acyl-transferase-like protein [Arabidopsis thaliana]
gi|332643586|gb|AEE77107.1| HXXXD-type
acyl-transferase-like protein [Arabidopsis thaliana]
Length = 442
Score = 73.2 bits (178), Expect = 3e-11
Identities = 41/104 (39%), Positives = 71/104 (68%), Gaps = 5/104 (4%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLP 185
I+ L++KA + +PTRV+ V+AL++KSAM ATR+ + G+S+PS+L +++RS + P
Sbjct: 224 IQALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVS-GTSKPSILANSVSLRSRVSPP 282
Query: 186 --QNAIGNIFSFNFCVNEDEGMD---FSALVGQLRKAKMKSKDL 302
+N+IGN+ S+ F +EG++ LV ++RKAK + +D+
Sbjct: 283 FTKNSIGNLVSY-FAAKAEEGINQTKLQTLVSKIRKAKQRFRDI 325
>ref|XP_006363208.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
tuberosum]
Length = 416
Score = 70.9 bits (172), Expect = 2e-10
Identities = 45/117 (38%), Positives = 72/117 (61%), Gaps = 9/117 (7%)
Frame = +3
Query: 3 EIEKLKSK-AVKSGIQKPTRVDVVTALLYKSAMVATRSTNCG--SSRPSVLLQPMNIRSL 173
+++ L+SK A +SG++ PTR +VV+ALL+K A+ AT ST + RPS + +N+R++
Sbjct: 210 KLDALRSKVAAESGVENPTRAEVVSALLFKCAIKATSSTTTSMLTRRPSKFVHFLNVRNM 269
Query: 174 IK--LPQNAIGNIFS-FNFCVNEDEGMDFSALVGQLRK---AKMKSKDLSEDELTYE 326
+K LPQ+AIGN+ S F+ ++ ++ LV LRK K + E+EL E
Sbjct: 270 MKSRLPQSAIGNLLSVFSITATNEQDIELPTLVRNLRKGVEVAYKKDQVEENELILE 326
>ref|XP_004232634.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
lycopersicum]
Length = 440
Score = 70.5 bits (171), Expect = 2e-10
Identities = 48/121 (39%), Positives = 74/121 (61%), Gaps = 6/121 (4%)
Frame = +3
Query: 3 EIEKLKSK-AVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIK 179
+++ L++K A +SG++ PTR +VV+ALL+K A+ AT ST S RPS + +N+RS++K
Sbjct: 237 KLDALRAKVAAESGVENPTRAEVVSALLFKCAIKATSSTTT-SMRPSKFVHLLNVRSMLK 295
Query: 180 --LPQNAIGNIF-SFNFCVNEDEGMDFSALVGQLRKA--KMKSKDLSEDELTYESQMQVL 344
LPQ+ IGNI SF ++E ++ LV LRK + KD E E ++V+
Sbjct: 296 SRLPQSTIGNILSSFTTTATKEEDIELPTLVRSLRKGVEEAHKKDHVEQN---EMMLEVV 352
Query: 345 E 347
E
Sbjct: 353 E 353
>ref|XP_004232635.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
lycopersicum]
Length = 437
Score = 70.1 bits (170), Expect = 3e-10
Identities = 42/96 (43%), Positives = 65/96 (67%), Gaps = 4/96 (4%)
Frame = +3
Query: 3 EIEKLKSK-AVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIK 179
+++ L++K A +SG++ PTR +VV+ALL+K A+ A ST S RPS + +N+RS++K
Sbjct: 234 KLDALRAKVAAESGVENPTRAEVVSALLFKCAIKAASSTTT-SMRPSKFVHLLNVRSMLK 292
Query: 180 --LPQNAIGNIF-SFNFCVNEDEGMDFSALVGQLRK 278
LPQ+ IGNI SF+ ++E ++ ALV LRK
Sbjct: 293 SRLPQSTIGNILSSFSTTATKEEDIELPALVRSLRK 328
>ref|XP_002267080.1| PREDICTED: vinorine synthase-like [Vitis vinifera]
Length = 429
Score = 69.7 bits (169), Expect = 4e-10
Identities = 38/90 (42%), Positives = 62/90 (68%), Gaps = 3/90 (3%)
Frame = +3
Query: 15 LKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI--KLPQ 188
LK+ A +SG++ PTRV+VV AL+Y+ A+ A++ + GS +PSVLLQ +N+R I LP+
Sbjct: 227 LKAMATQSGVENPTRVEVVAALIYRCAVAASK-VSSGSLKPSVLLQTVNLRKRIVPPLPE 285
Query: 189 NAIGN-IFSFNFCVNEDEGMDFSALVGQLR 275
+ GN ++ ++ + ++F ALVGQL+
Sbjct: 286 KSFGNMVWFYSIHTARESEIEFHALVGQLK 315
>emb|CAN63243.1| hypothetical protein VITISV_028808 [Vitis vinifera]
Length = 429
Score = 69.7 bits (169), Expect = 4e-10
Identities = 38/90 (42%), Positives = 62/90 (68%), Gaps = 3/90 (3%)
Frame = +3
Query: 15 LKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI--KLPQ 188
LK+ A +SG++ PTRV+VV AL+Y+ A+ A++ + GS +PSVLLQ +N+R I LP+
Sbjct: 227 LKAMATQSGVENPTRVEVVAALIYRCAVAASK-VSSGSLKPSVLLQTVNLRKRIVPPLPE 285
Query: 189 NAIGN-IFSFNFCVNEDEGMDFSALVGQLR 275
+ GN ++ ++ + ++F ALVGQL+
Sbjct: 286 KSFGNMVWFYSIHTARESEIEFHALVGQLK 315
>emb|CAN69388.1| hypothetical protein VITISV_027664 [Vitis vinifera]
Length = 429
Score = 69.7 bits (169), Expect = 4e-10
Identities = 38/90 (42%), Positives = 62/90 (68%), Gaps = 3/90 (3%)
Frame = +3
Query: 15 LKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI--KLPQ 188
LK+ A +SG++ PTRV+VV AL+Y+ A+ A++ + GS +PSVLLQ +N+R I LP+
Sbjct: 227 LKAMATQSGVENPTRVEVVAALIYRCAVAASK-VSSGSLKPSVLLQTVNLRKRIVPPLPE 285
Query: 189 NAIGN-IFSFNFCVNEDEGMDFSALVGQLR 275
+ GN ++ ++ + ++F ALVGQL+
Sbjct: 286 KSFGNMVWFYSIHTARESEIEFHALVGQLK 315
>ref|XP_006351214.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
tuberosum]
Length = 461
Score = 68.6 bits (166), Expect = 8e-10
Identities = 46/116 (39%), Positives = 75/116 (64%), Gaps = 5/116 (4%)
Frame = +3
Query: 3 EIEKLKSKAV-KSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI- 176
++EKLK++ ++G+Q PTR +V++AL YK A A R+ N S +PS+LL P+N+R ++
Sbjct: 258 KLEKLKTQVESETGVQNPTRFEVLSALFYKCAATAARA-NSSSFKPSMLLLPVNLRPILD 316
Query: 177 -KLPQNAIGNIFSF--NFCVNEDEGMDFSALVGQLRKAKMKSKDLSEDELTYESQM 335
LP AIGNI S ++EDE M + +V ++RKAK +L +++ ES++
Sbjct: 317 PPLPTRAIGNILSMIKVTAMSEDE-MTIARVVREIRKAK---DELKKEDHVKESKL 368
>ref|XP_004140566.1| PREDICTED: BAHD acyltransferase At5g47980-like [Cucumis sativus]
gi|449487369|ref|XP_004157592.1| PREDICTED: BAHD
acyltransferase At5g47980-like [Cucumis sativus]
Length = 433
Score = 68.6 bits (166), Expect = 8e-10
Identities = 41/98 (41%), Positives = 65/98 (66%), Gaps = 5/98 (5%)
Frame = +3
Query: 3 EIEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIK- 179
+I LK+KA S ++ PTRV+ + ALL+KSA+ A+RST+ G SR SVL Q +N+R ++K
Sbjct: 220 KIASLKAKAASSTVKNPTRVEAIAALLWKSAVSASRSTS-GVSRASVLGQAVNLRKILKP 278
Query: 180 -LPQNAIGNIFSF---NFCVNEDEGMDFSALVGQLRKA 281
LP+N++GN F V + ++ ++VG +R+A
Sbjct: 279 NLPENSVGNAIGFVTPETAVGAE--LELQSMVGLMREA 314
>ref|XP_002875299.1| transferase family protein [Arabidopsis lyrata subsp. lyrata]
gi|297321137|gb|EFH51558.1| transferase family protein
[Arabidopsis lyrata subsp. lyrata]
Length = 442
Score = 67.8 bits (164), Expect = 1e-09
Identities = 40/103 (38%), Positives = 67/103 (65%), Gaps = 5/103 (4%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLP 185
I+ L++KA + +PTRV+ V+AL++K+AM ATR+ + G+S+PS+L ++RS + P
Sbjct: 224 IQALQAKASSFEVNQPTRVEAVSALIWKTAMKATRTVS-GTSKPSILANSASLRSRVSPP 282
Query: 186 --QNAIGNIFSFNFCVNEDEGMD---FSALVGQLRKAKMKSKD 299
+N+IGN+ S+ F +EG + LV ++RKAK +D
Sbjct: 283 FTKNSIGNLVSY-FAAKAEEGTNQTKLQTLVSKIRKAKQWFRD 324
>ref|XP_006292162.1| hypothetical protein CARUB_v10018368mg [Capsella rubella]
gi|482560869|gb|EOA25060.1| hypothetical protein
CARUB_v10018368mg [Capsella rubella]
Length = 431
Score = 67.4 bits (163), Expect = 2e-09
Identities = 38/103 (36%), Positives = 68/103 (66%), Gaps = 5/103 (4%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLP 185
I+ L++KA + +PTRV+ V+AL++K+A+ ATR+ + G+S+PS+L ++RS + P
Sbjct: 224 IQSLQAKASSFEVNQPTRVEAVSALIWKTAVKATRTVS-GTSKPSILANSASLRSRLSPP 282
Query: 186 --QNAIGNIFSFNFCVNEDEGMD---FSALVGQLRKAKMKSKD 299
+N IGN+ S+ F +EG++ LV ++RKAK + ++
Sbjct: 283 FTENTIGNLVSY-FAAKAEEGINQTKLQTLVSEIRKAKQRFQE 324
>ref|XP_006470642.1| PREDICTED: BAHD acyltransferase At5g47980-like [Citrus sinensis]
Length = 466
Score = 67.0 bits (162), Expect = 2e-09
Identities = 35/96 (36%), Positives = 64/96 (66%), Gaps = 2/96 (2%)
Frame = +3
Query: 3 EIEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI-- 176
++ +L+++ + + +P+RV+ +TAL++K A A+RS N G +RPS+L+Q +N+RS++
Sbjct: 244 KLAQLQAEVASASVPRPSRVEALTALIWKCARTASRS-NRGFARPSLLVQAVNLRSVVVP 302
Query: 177 KLPQNAIGNIFSFNFCVNEDEGMDFSALVGQLRKAK 284
L +N++GN F ++ M+ LV +LRKAK
Sbjct: 303 PLSENSVGNSIGFMPAQTSEKEMELQELVCKLRKAK 338
>ref|XP_006446155.1| hypothetical protein CICLE_v10015146mg [Citrus clementina]
gi|557548766|gb|ESR59395.1| hypothetical protein
CICLE_v10015146mg [Citrus clementina]
Length = 466
Score = 67.0 bits (162), Expect = 2e-09
Identities = 35/96 (36%), Positives = 64/96 (66%), Gaps = 2/96 (2%)
Frame = +3
Query: 3 EIEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLI-- 176
++ +L+++ + + +P+RV+ +TAL++K A A+RS N G +RPS+L+Q +N+RS++
Sbjct: 244 KLAQLQAEVASASVPRPSRVEALTALIWKCARTASRS-NRGFARPSLLVQAVNLRSVVVP 302
Query: 177 KLPQNAIGNIFSFNFCVNEDEGMDFSALVGQLRKAK 284
L +N++GN F ++ M+ LV +LRKAK
Sbjct: 303 PLSENSVGNSIGFMPAQTSEKEMELQELVCKLRKAK 338
>ref|XP_006362683.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
tuberosum]
Length = 440
Score = 66.2 bits (160), Expect = 4e-09
Identities = 35/88 (39%), Positives = 59/88 (67%), Gaps = 2/88 (2%)
Frame = +3
Query: 27 AVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLPQNAIGNI 206
A +S +Q PTR +VV+AL++K A+ A ++ + G +PS ++Q +++R+L+ LP NAIGN+
Sbjct: 244 AAESEVQNPTRTEVVSALIFKCAVRAAKAKS-GIFQPSSIVQAVDLRALVGLPPNAIGNL 302
Query: 207 FSF--NFCVNEDEGMDFSALVGQLRKAK 284
+ N +E M S LV ++RK+K
Sbjct: 303 LTICPTSITNNEESMTISKLVTEMRKSK 330
>ref|XP_006344158.1| PREDICTED: deacetylvindoline O-acetyltransferase-like [Solanum
tuberosum]
Length = 430
Score = 66.2 bits (160), Expect = 4e-09
Identities = 35/88 (39%), Positives = 59/88 (67%), Gaps = 2/88 (2%)
Frame = +3
Query: 27 AVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIKLPQNAIGNI 206
A +S +Q PTR +VV+AL++K A+ A ++ + G +PS ++Q +++R+L+ LP NAIGN+
Sbjct: 234 AAESEVQNPTRTEVVSALIFKCAVRAAKAKS-GIFQPSSIVQAVDLRALVGLPPNAIGNL 292
Query: 207 FSF--NFCVNEDEGMDFSALVGQLRKAK 284
+ N +E M S LV ++RK+K
Sbjct: 293 LTICPTSITNNEESMTISKLVTEMRKSK 320
>ref|XP_006471129.1| PREDICTED: BAHD acyltransferase At5g47980-like [Citrus sinensis]
Length = 456
Score = 65.9 bits (159), Expect = 5e-09
Identities = 39/99 (39%), Positives = 64/99 (64%), Gaps = 5/99 (5%)
Frame = +3
Query: 3 EIEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNIRSLIK- 179
+I++L++K + + +P+RV+V+TAL++K A A+RS N G SRPS+ + MN+R++ K
Sbjct: 230 KIDQLRAKVASASVPRPSRVEVLTALIWKCAKAASRS-NLGYSRPSLSVHAMNVRAVAKP 288
Query: 180 -LPQNAIGNIFSFNFCVNEDEGMDFSA---LVGQLRKAK 284
LP+N++GN S + + G + LV LRKAK
Sbjct: 289 PLPENSVGN--SVGYVTAQASGNEIETVQDLVLSLRKAK 325
>ref|XP_004295692.1| PREDICTED: BAHD acyltransferase At5g47980-like [Fragaria vesca
subsp. vesca]
Length = 442
Score = 65.9 bits (159), Expect = 5e-09
Identities = 42/116 (36%), Positives = 63/116 (54%), Gaps = 2/116 (1%)
Frame = +3
Query: 6 IEKLKSKAVKSGIQKPTRVDVVTALLYKSAMVATRSTNCGSSRPSVLLQPMNI--RSLIK 179
I LKSKA + + PTRV+VV+AL++K M A+RS GS RPS+ Q +N+ RS
Sbjct: 238 IAALKSKAASASVPNPTRVEVVSALIWKCTMEASRS-KLGSIRPSLWCQAVNMRKRSGQA 296
Query: 180 LPQNAIGNIFSFNFCVNEDEGMDFSALVGQLRKAKMKSKDLSEDELTYESQMQVLE 347
+N +GN + + + +D +LV + RK K K + LT E Q ++
Sbjct: 297 FTENLLGNFVWYLAAITVESEVDIQSLVAKFRKCKDDFKGKYRNGLTAEDAYQTIK 352