BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rauwolfia21_contig00003255
         (1847 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   382   0.0  
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   367   0.0  
ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   369   0.0  
ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   371   0.0  
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   367   0.0  
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   360   0.0  
gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus...   357   0.0  
ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   354   0.0  
gb|EMJ11091.1| hypothetical protein PRUPE_ppa004048mg [Prunus pe...   369   0.0  
ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   352   0.0  
ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   340   e-178
ref|XP_006649485.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   341   e-178
ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [S...   340   e-178
gb|EEC74610.1| hypothetical protein OsI_10221 [Oryza sativa Indi...   335   e-177
ref|NP_001049129.1| Os03g0174600 [Oryza sativa Japonica Group] g...   332   e-176
gb|ACG45558.1| tRNA methyltransferase [Zea mays]                      332   e-176
ref|XP_004985520.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   330   e-175
ref|NP_001131207.1| uncharacterized protein LOC100192515 [Zea ma...   331   e-175
gb|EMT11416.1| tRNA-specific 2-thiouridylase mnmA [Aegilops taus...   329   e-173
dbj|BAK01046.1| predicted protein [Hordeum vulgare subsp. vulgare]    323   e-171

>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  382 bits (981), Expect(2) = 0.0
 Identities = 182/237 (76%), Positives = 205/237 (86%), Gaps = 1/237 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVRKLA    LPN+DRKDSQGICFLGKIKFSEFVARHIGE EGVILEAESGDFLGKHRG
Sbjct: 259  DEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRG 318

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKD+KNNVVFVSRNYFS DKRRR FR+GS KWLSG  P
Sbjct: 319  FWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPP 378

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKE-IGVVHLSQDDQGLAAGQYATFYDGTTCI 507
             +  +LQCKVRHGP FYSC L+ME SE G E + VVHLS+DDQGLAAGQ+A FY G +CI
Sbjct: 379  DQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCI 438

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENCIDETDLRDQTQL 336
            GSGVILESWDD+GFPVC KA+E A++ED++KLGKP+KI VK E+ + E+  +D T++
Sbjct: 439  GSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEI 495



 Score =  343 bits (881), Expect(2) = 0.0
 Identities = 178/278 (64%), Positives = 202/278 (72%), Gaps = 12/278 (4%)
 Frame = -2

Query: 1846 LRLSATMSPALSFALKHLFSFSFSPHICKPKTLLF-PHILNSR---------TAVSPPSR 1697
            LRL    +   S  + H  S   +P I +P      P +  +R         T++S  S+
Sbjct: 3    LRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSSK 62

Query: 1696 PLNSSNFRPLCSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVALRL 1517
            PL+ ++F P                     +LSC MP   PLKVAVLLSGGVDSSVALRL
Sbjct: 63   PLSDADFLP---------------------FLSCSMPTY-PLKVAVLLSGGVDSSVALRL 100

Query: 1516 LHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNN 1337
            LH AGHSCTAFYLKIWFQEDFENFWSECPW+DDLKYAKAVCDQVDVPLEVVHLT+EYW N
Sbjct: 101  LHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRN 160

Query: 1336 VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFH--ASTWDE 1163
            VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDA+ +M FD++ASGHYA V H  A   D+
Sbjct: 161  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDK 220

Query: 1162 PSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            PS+LELS+DMVKDQTYFLSHLSQ QL+RL+FPLGCIPK
Sbjct: 221  PSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPK 258


>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  367 bits (941), Expect(2) = 0.0
 Identities = 176/223 (78%), Positives = 194/223 (86%), Gaps = 1/223 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            +EVRK A    LPN+DRKDSQGICFLGKIKFSEFVARHIGE EGVILEAE+GDFLGKH G
Sbjct: 247  EEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILEAETGDFLGKHWG 306

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S DK+RR FR+GS KWLSG  P
Sbjct: 307  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFRVGSLKWLSGLPP 366

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKE-IGVVHLSQDDQGLAAGQYATFYDGTTCI 507
             +I +LQCKVRHGPGFY+C L +E  E G E I VV LS+DDQGLAAGQ+A FY GT CI
Sbjct: 367  EEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAGQFAAFYQGTRCI 426

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQE 378
            GSGVILESWDD+GFPVC KALE+AR+ED++KLGKP+KI VK E
Sbjct: 427  GSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPE 469



 Score =  347 bits (889), Expect(2) = 0.0
 Identities = 182/266 (68%), Positives = 204/266 (76%), Gaps = 2/266 (0%)
 Frame = -2

Query: 1828 MSPALSFALKHLFSFSFSPHICKPKTLLFPHILNSRTAVSPPSRPLNSSNFRPLCSISNS 1649
            MS  LS ++K L S   S    KP+TL   H  N +       RP++       CS+S +
Sbjct: 7    MSMTLS-SVKPLLSLFLSR---KPRTLFHSH--NPKLIFL--RRPIS-------CSVSTA 51

Query: 1648 DNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIW 1469
                    T I+PRYLSC MP +  LKVAVLLSGGVDSSVALRLLH AGHSCTAFYLKIW
Sbjct: 52   -------ATQIEPRYLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIW 104

Query: 1468 FQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPN 1289
            FQEDFENFW+ECPWE+D+KYAKAVCDQVDVPLEVVHLTDEYW NVVSYIIEEYRCGRTPN
Sbjct: 105  FQEDFENFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPN 164

Query: 1288 PDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFHASTWDE--PSLLELSRDMVKDQTY 1115
            PDVLCNTRIKFGAFMDA+  M FD++ASGHYAKV H+S   E  PS+LELS+D VKDQTY
Sbjct: 165  PDVLCNTRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTY 224

Query: 1114 FLSHLSQTQLRRLIFPLGCIPKVRMK 1037
            FLSHLSQ QL+RLIFPLGCI K  ++
Sbjct: 225  FLSHLSQAQLKRLIFPLGCISKEEVR 250


>ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Glycine max]
          Length = 502

 Score =  369 bits (946), Expect(2) = 0.0
 Identities = 178/238 (74%), Positives = 199/238 (83%), Gaps = 4/238 (1%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVRKLA    LPN+DRKDSQGICFLGKI+FSEFVARHIGE EGVILEAE+GDFLGKHRG
Sbjct: 253  DEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILEAETGDFLGKHRG 312

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DKRRR FR+GSFKWLSG  P
Sbjct: 313  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSGLPP 372

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGK-EIGVVHLSQDDQGLAAGQYATFYDGTTCI 507
             +  QLQCKVRHGPGFY C+L ME    G+    VV +S+DDQGLAAGQ+A FY+G TCI
Sbjct: 373  GQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSAVVRISEDDQGLAAGQFAAFYEGRTCI 432

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQEN---CIDETDLRDQT 342
            GSGVILE WDD+ FPVC+KALE+AR+ED++K+G PVKI VK +N     D  +L  +T
Sbjct: 433  GSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPVKIKVKPDNPQEVCDSAELASKT 490



 Score =  337 bits (864), Expect(2) = 0.0
 Identities = 157/189 (83%), Positives = 173/189 (91%), Gaps = 2/189 (1%)
 Frame = -2

Query: 1609 RYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECP 1430
            RYL C MP+ SPL+VAVL+SGGVDSSVALRLLH AGHSCTAFYLKIWFQEDFENFWSECP
Sbjct: 64   RYLRCSMPQNSPLRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECP 123

Query: 1429 WEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGA 1250
            WEDDLKYAK VC+QVDVPLEVVHLTDEYWNNVVSY+IEEY  GRTPNPDVLCNTRIKFGA
Sbjct: 124  WEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIKFGA 183

Query: 1249 FMDAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRL 1076
            F+DA+  MGFD++ASGHYA V H  +   DEPS+LELS+DMVKDQTYFLSHLSQ+QL++L
Sbjct: 184  FLDAIGGMGFDYVASGHYANVIHPCSDRRDEPSVLELSQDMVKDQTYFLSHLSQSQLKQL 243

Query: 1075 IFPLGCIPK 1049
            +FPLGCIPK
Sbjct: 244  LFPLGCIPK 252


>ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cicer arietinum]
          Length = 484

 Score =  371 bits (952), Expect(2) = 0.0
 Identities = 178/238 (74%), Positives = 203/238 (85%), Gaps = 1/238 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA    LPN+DRKDSQGICFLGKIKFSEFVARHIGE EG+ILEAE+GDFLGKHRG
Sbjct: 246  DEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGKHRG 305

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS+DKRRR FR+GS KWLSG  P
Sbjct: 306  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFRVGSLKWLSGLPP 365

Query: 683  SKIDQLQCKVRHGPGFYSCTLSME-TSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCI 507
            S+  QL+CKVRHGPGFY C+L ME   E   +  VV +S+DDQGLAAGQ+  FY+G TCI
Sbjct: 366  SQTSQLRCKVRHGPGFYDCSLQMELEGENPIDSAVVRISEDDQGLAAGQFTAFYEGRTCI 425

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENCIDETDLRDQTQLA 333
            GSGVILESWDD+GFPVC+KALE+AR+ED++K+G PVKI VK +N     ++ D T+LA
Sbjct: 426  GSGVILESWDDQGFPVCTKALEIARMEDKSKIGNPVKIKVKPDN---PQEVCDSTELA 480



 Score =  333 bits (855), Expect(2) = 0.0
 Identities = 158/196 (80%), Positives = 175/196 (89%), Gaps = 2/196 (1%)
 Frame = -2

Query: 1630 VQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFE 1451
            +   +DP YL C MP  +PL+VAVL+SGGVDSSVALRLLH AGHSCTAFYLKIWFQEDFE
Sbjct: 52   INGQLDP-YLRCSMPH-NPLRVAVLISGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 109

Query: 1450 NFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCN 1271
            NFWSECPWEDDLKYAK VC+QV+VPLEVVHLTDEYW NVVSYIIEEY+CGRTPNPDVLCN
Sbjct: 110  NFWSECPWEDDLKYAKDVCNQVEVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCN 169

Query: 1270 TRIKFGAFMDAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRDMVKDQTYFLSHLS 1097
            TRIKFGAF+DA+ DMGFD++ASGHYA V H  A   D PS LELS+DMVKDQTYFLSHLS
Sbjct: 170  TRIKFGAFLDAISDMGFDYVASGHYANVIHPSADQMDGPSTLELSQDMVKDQTYFLSHLS 229

Query: 1096 QTQLRRLIFPLGCIPK 1049
            Q+QL++L+FPLGCIPK
Sbjct: 230  QSQLKQLLFPLGCIPK 245


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  367 bits (942), Expect(2) = 0.0
 Identities = 178/233 (76%), Positives = 195/233 (83%), Gaps = 1/233 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            +EVR LA    LPN+DRKDSQGICFLGKIKFSEFV RHIGE+EGVILEAE+GDFLG HRG
Sbjct: 263  EEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVILEAETGDFLGIHRG 322

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS+DKRRRSFR+GS KWLSGS  
Sbjct: 323  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSPT 382

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKG-KEIGVVHLSQDDQGLAAGQYATFYDGTTCI 507
                QLQCKVRHGP FY C++ +E  E   +E+  VHL +DDQGLAAGQ+  FY G TCI
Sbjct: 383  DMTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQGLAAGQFTAFYQGRTCI 442

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENCIDETDLRD 348
            GSGVILESWDDKGFPVC KALELARLED++KLGKPVKI VK E    E+  +D
Sbjct: 443  GSGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKD 495



 Score =  337 bits (864), Expect(2) = 0.0
 Identities = 173/275 (62%), Positives = 206/275 (74%), Gaps = 5/275 (1%)
 Frame = -2

Query: 1846 LRLSATMSPALSFALKHLFSFSFSPHICKPKTLLFPHILNSRTAVSPPSRPLNSSNF--R 1673
            LRL A ++P L  +L     FS  P +  P     P    S  + +P    L S +F  +
Sbjct: 3    LRLGA-LTPLLRPSL-----FSPKPILLSPLITAKPFFSLSSFSTAPFQSSLVSHSFNPK 56

Query: 1672 PLCSISNSDNVVEKVQTSIDPRYLSCCMPERS-PLKVAVLLSGGVDSSVALRLLHLAGHS 1496
            P     +  N +     S+DP YLSCCM ++  PL++AVLLSGGVDSSVALRLLH AGH 
Sbjct: 57   PTTLFDSGKNAI-----SVDPYYLSCCMADKDKPLRIAVLLSGGVDSSVALRLLHAAGHH 111

Query: 1495 CTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIE 1316
            CTAFYLKIWFQEDF NFWSECPWE+DLK+AK VCDQV VPLEVVHLTDEYW+NVVSYIIE
Sbjct: 112  CTAFYLKIWFQEDFNNFWSECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIE 171

Query: 1315 EYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELS 1142
            EYRCGRTPNPDVLCNTRIKFGAFMDA+++M FD++ASGHYA V H  +   ++PS+LELS
Sbjct: 172  EYRCGRTPNPDVLCNTRIKFGAFMDAINNMEFDYVASGHYAHVIHPVSDQINKPSVLELS 231

Query: 1141 RDMVKDQTYFLSHLSQTQLRRLIFPLGCIPKVRMK 1037
            +D +KDQTYFLSHL+Q QL+RLIFPLGCIPK  ++
Sbjct: 232  KDKLKDQTYFLSHLTQAQLKRLIFPLGCIPKEEVR 266


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  360 bits (925), Expect(2) = 0.0
 Identities = 170/233 (72%), Positives = 198/233 (84%), Gaps = 1/233 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVRKLA    LPN+DRKDSQGICFLGKIKFSEFVARHIGE EG++LEAE+GDFLG HRG
Sbjct: 248  DEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLEAETGDFLGNHRG 307

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DKRRR  R+GS +W +GS P
Sbjct: 308  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLRVGSLRWFNGSPP 367

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSE-KGKEIGVVHLSQDDQGLAAGQYATFYDGTTCI 507
             +  +LQCKVRHGPGFY+C++ +E  E   +++ +V L +DDQGLAAGQ+A FY G TCI
Sbjct: 368  KETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAGQFAAFYKGRTCI 427

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENCIDETDLRD 348
            GSGVILESW+D+GFPVC +ALELAR+ED+++LGKPVKI VK E  + E D +D
Sbjct: 428  GSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPEIPVREFDGKD 480



 Score =  342 bits (876), Expect(2) = 0.0
 Identities = 179/269 (66%), Positives = 201/269 (74%), Gaps = 3/269 (1%)
 Frame = -2

Query: 1846 LRLSATMSPA-LSFALKHLFSFSFSPHICKPKTLLFPHILNSRTAVSPPSRPLNSSNFRP 1670
            LR  A + P  LS  LK L    F P     K L F     SR +    S    +SN   
Sbjct: 2    LRAMAMVRPVTLSLHLKPLLPCYFPP-----KPLHF-----SRLSKLVQSLTTRASNNNA 51

Query: 1669 LCSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCT 1490
             C I             I+P YLSCC+P+++ L++A+LLSGGVDSSVALRLLH AGH CT
Sbjct: 52   GCGI-------------IEPNYLSCCLPDKNNLRIALLLSGGVDSSVALRLLHAAGHHCT 98

Query: 1489 AFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEY 1310
            AFYLKIWFQEDFENFWSECPWE+DLKYAKAVCDQVD+PLEVVHLTDEYW NVVSYIIEEY
Sbjct: 99   AFYLKIWFQEDFENFWSECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEY 158

Query: 1309 RCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRD 1136
            RCGRTPNPDVLCNTRIKFGAFMDA+++M FD++ASGHYAKV H  A   ++PS+LELS+D
Sbjct: 159  RCGRTPNPDVLCNTRIKFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKD 218

Query: 1135 MVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            MVKDQTYFLSHLSQ QLRRLIFPLG I K
Sbjct: 219  MVKDQTYFLSHLSQAQLRRLIFPLGGISK 247


>gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus vulgaris]
          Length = 488

 Score =  357 bits (916), Expect(2) = 0.0
 Identities = 169/224 (75%), Positives = 192/224 (85%), Gaps = 1/224 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA    LPN+DRKDSQGICFLGKI+FSEFVARHIGE EG+ILEAE+GDFLGKHRG
Sbjct: 251  DEVRRLATEFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGIILEAETGDFLGKHRG 310

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DKRRR FR+GSFKWLS   P
Sbjct: 311  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSELPP 370

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGK-EIGVVHLSQDDQGLAAGQYATFYDGTTCI 507
             +  QLQCKVRHGPGFY C+L ME    G+    VV +S+DDQGLAAGQ+A FY+G TC+
Sbjct: 371  GQTSQLQCKVRHGPGFYDCSLQMEVEGDGQGHSAVVRISEDDQGLAAGQFAAFYEGRTCL 430

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQEN 375
            GSGVILE WD++ FPVC+KALE+AR+ED++ LG PVKI VK ++
Sbjct: 431  GSGVILEFWDEQSFPVCTKALEIARMEDKSILGNPVKIKVKPDS 474



 Score =  337 bits (865), Expect(2) = 0.0
 Identities = 168/233 (72%), Positives = 189/233 (81%), Gaps = 3/233 (1%)
 Frame = -2

Query: 1738 HILNSRTAVSPPSRPLNSSNFRPLCSISNSDNVVEKVQTSID-PRYLSCCMPERSPLKVA 1562
            H LN R    PP R +     R  C  S+S      +  S D  RYL C +P+ SPL+VA
Sbjct: 27   HTLNPR----PPCRTV-----RLFCRPSSSAVAHTSLPLSADLHRYLRCSLPQNSPLRVA 77

Query: 1561 VLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVD 1382
            VL+SGGVDSSVALRLLH AGHSCTAFYLKIWFQEDFENFWSECPWE+DLKYAKAVC+QVD
Sbjct: 78   VLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCEQVD 137

Query: 1381 VPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASG 1202
            VPLEVVHLTDEYWNNVVSY+IEEY  GRTPNPDVLCNTRIKFGAF+DA+  MGFD++ASG
Sbjct: 138  VPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIKFGAFLDAIGGMGFDYVASG 197

Query: 1201 HYAKVFH--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            HYA V H  A   + PS+LELS+DMVKDQTYFLSHLSQ+QL++L+FPLGCIPK
Sbjct: 198  HYANVIHPCADGMNGPSVLELSQDMVKDQTYFLSHLSQSQLKQLLFPLGCIPK 250


>ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Fragaria vesca
            subsp. vesca]
          Length = 475

 Score =  354 bits (909), Expect(2) = 0.0
 Identities = 167/225 (74%), Positives = 193/225 (85%), Gaps = 1/225 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            D+VRKLA    LPN++RKDSQGICFLGK++FSEF+ +HIGE EGVILEAE+GD LGKHRG
Sbjct: 251  DKVRKLATKFDLPNKERKDSQGICFLGKVRFSEFIGKHIGEKEGVILEAETGDLLGKHRG 310

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKD+K+NVVFVSRNY+SVDKRRR FR+GS KWL GS P
Sbjct: 311  FWFYTIGQRQGLRLPGGPWYVVEKDIKHNVVFVSRNYYSVDKRRRLFRVGSLKWLHGSPP 370

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKE-IGVVHLSQDDQGLAAGQYATFYDGTTCI 507
            ++I QLQCKVRHGP F +CT+ +E  E G E + VV LS+DDQGLAAGQ+A FY G TC+
Sbjct: 371  AQISQLQCKVRHGPRFCNCTVKIEHGEDGNEDVAVVSLSEDDQGLAAGQFAAFYQGRTCL 430

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENC 372
            GSGVILESWDD+GFPVC+KALE AR+ED++ LGKPVKI    E C
Sbjct: 431  GSGVILESWDDQGFPVCTKALENARMEDKSLLGKPVKIKFIPEAC 475



 Score =  336 bits (862), Expect(2) = 0.0
 Identities = 176/272 (64%), Positives = 197/272 (72%), Gaps = 11/272 (4%)
 Frame = -2

Query: 1819 ALSFALKHLFSF--------SFSPHICKPKTLLFPHILNSRTAVSP-PSRPLNSSNFRPL 1667
            A++  L H+ S         S S H  KP   LF    +   A+S  P  P NS N    
Sbjct: 2    AMTSLLSHITSLPLPLKRPSSLSSHSPKPTKTLFLKSFSISAAISQNPEPPPNSINV--- 58

Query: 1666 CSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTA 1487
              I +               YLSC MP + PLKVAVLLSGGVDSSVALRLLH AGHSCTA
Sbjct: 59   -DIGS---------------YLSCSMPHKKPLKVAVLLSGGVDSSVALRLLHAAGHSCTA 102

Query: 1486 FYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYR 1307
            FYLKIWFQE FENFWSECPWE+DL YAKAVC+QVDVPLEVVHLTDEYW NVVSYIIEEYR
Sbjct: 103  FYLKIWFQEGFENFWSECPWEEDLNYAKAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYR 162

Query: 1306 CGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRDM 1133
            CGRTPNPDVLCNTRIKFG FMDA+D M FD++ASGHYAKV H  A   D+ S+LELS+DM
Sbjct: 163  CGRTPNPDVLCNTRIKFGVFMDAIDSMEFDYVASGHYAKVVHLSADQTDKDSVLELSQDM 222

Query: 1132 VKDQTYFLSHLSQTQLRRLIFPLGCIPKVRMK 1037
            VKDQTYFLSHLSQ QL++LIFPLGC+ K +++
Sbjct: 223  VKDQTYFLSHLSQDQLKKLIFPLGCLSKDKVR 254


>gb|EMJ11091.1| hypothetical protein PRUPE_ppa004048mg [Prunus persica]
          Length = 533

 Score =  369 bits (947), Expect(2) = 0.0
 Identities = 177/239 (74%), Positives = 203/239 (84%), Gaps = 1/239 (0%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVRKLA    L N+ RKDSQGICFLGK+KFSEF+ARHIGE EG+ILEAE+GDFLG HRG
Sbjct: 254  DEVRKLATKFDLLNKQRKDSQGICFLGKLKFSEFIARHIGEKEGIILEAETGDFLGTHRG 313

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKD+KNNVVFVSRNYFSVDKRRR FR+GS KWL+G   
Sbjct: 314  FWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPQ 373

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKE-IGVVHLSQDDQGLAAGQYATFYDGTTCI 507
            ++I QLQCKVRHGPGF +C+L ME    G E + VVHLS+DDQGLAAGQ+A FY G TC+
Sbjct: 374  NQISQLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCL 433

Query: 506  GSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIMVKQENCIDETDLRDQTQLAQ 330
            GSGVILESWDD+GFPVC++ALE+AR+ED++ LGKPVKI VK EN I E+D  D  +L +
Sbjct: 434  GSGVILESWDDQGFPVCARALEIARMEDKSLLGKPVKIKVKPENPITESDQVDGMELCR 492



 Score =  321 bits (822), Expect(2) = 0.0
 Identities = 171/247 (69%), Positives = 193/247 (78%), Gaps = 5/247 (2%)
 Frame = -2

Query: 1774 PH-ICKPKTLLFPHILN-SRTA-VSPPSRPLNSSNFRPLCSISNSDNVVEKVQTSIDPRY 1604
            PH I K    LFPH  N +RT  + P SR  +SS+     ++  + +    +   + P Y
Sbjct: 18   PHQILKLHLSLFPHSPNPARTLFLGPLSRCCSSSS-----AVPQTPHPPNSINGDLGP-Y 71

Query: 1603 LSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWE 1424
            LSC MP +S LKVAVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQEDFENFWSECPWE
Sbjct: 72   LSCSMPHQS-LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWE 130

Query: 1423 DDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 1244
            +DLKYAKAVC+QVDVPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM
Sbjct: 131  EDLKYAKAVCNQVDVPLEVVHLTNEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 190

Query: 1243 DAVDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIF 1070
            DA+  M FD++ASGHYA V H  A   DE S+LELS+DM    TYFLSHLSQ QL++LIF
Sbjct: 191  DAISCMKFDYVASGHYANVVHPSAGEMDEDSVLELSQDM----TYFLSHLSQAQLKQLIF 246

Query: 1069 PLGCIPK 1049
            PLGC+ K
Sbjct: 247  PLGCLSK 253


>ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
            gi|297340151|gb|EFH70568.1| tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
          Length = 500

 Score =  352 bits (902), Expect(2) = 0.0
 Identities = 164/217 (75%), Positives = 186/217 (85%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVRKLA    LPN+DRKDSQGICFLGKIKFS+FV  HIGE EG+ILEAE+GDFLG HRG
Sbjct: 262  DEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCAHIGEMEGIILEAETGDFLGNHRG 321

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S+DKRRR FR+GS +WLSG   
Sbjct: 322  FWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWLSGKPS 381

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
              + +L+CKVRHGP FYSC+  ME      ++ VVHL +DDQGLAAGQ+A FY+GTTCIG
Sbjct: 382  GNVRELRCKVRHGPSFYSCSFEMEAD---GDVAVVHLDEDDQGLAAGQFAAFYEGTTCIG 438

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKI 393
            SGVILESWDD+ FPVC+KAL+LA +ED+TKLGKPVKI
Sbjct: 439  SGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPVKI 475



 Score =  328 bits (841), Expect(2) = 0.0
 Identities = 163/228 (71%), Positives = 185/228 (81%), Gaps = 10/228 (4%)
 Frame = -2

Query: 1702 SRPLNSSNFRPLCSISNSDNVVEKVQTSID--------PRYLSCCMPERSPLKVAVLLSG 1547
            S P +SS+  P  S  +SD +   + + ++         +YLSC MPE+ PL+VAVLLSG
Sbjct: 37   SSPSSSSSLLP--SPQSSDPITPLLSSGLNYSRHAFLLDQYLSCSMPEK-PLRVAVLLSG 93

Query: 1546 GVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEV 1367
            GVDSSVALRLLH AGHSCTAFYLKIWFQE FENFW++CPWEDDLKYAK VC+QVDVPLEV
Sbjct: 94   GVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWEDDLKYAKHVCEQVDVPLEV 153

Query: 1366 VHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKV 1187
            VHLTDEYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDA+ DM +D++ SGHYAKV
Sbjct: 154  VHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISDMEYDYVGSGHYAKV 213

Query: 1186 FH--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
             H      D  S+LELS+DMVKDQTYFLSHLSQTQL+RLIFPLGC+ K
Sbjct: 214  VHPPVDQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLIFPLGCVKK 261


>ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Brachypodium
            distachyon]
          Length = 481

 Score =  340 bits (872), Expect(2) = e-178
 Identities = 154/218 (70%), Positives = 188/218 (86%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE EGV+LEAE+GD+LG+HRG
Sbjct: 248  DEVRSLATQMDLPNQGRKDSQGICFLGKVKFSEFVERHIGEMEGVLLEAETGDYLGRHRG 307

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 308  FWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGP 367

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               +QL+CKVRH P F++CT++ E +E+  ++  VHLS+DDQGLAAGQ+A FY   TC+G
Sbjct: 368  ENDEQLKCKVRHSPEFHNCTVTREQTEESGDVLAVHLSEDDQGLAAGQFAAFYRDNTCLG 427

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVC+ ALE+AR+ED++KLGKPV+I+
Sbjct: 428  SGIILDSWDEMSFPVCANALEIARMEDKSKLGKPVRIV 465



 Score =  315 bits (806), Expect(2) = e-178
 Identities = 163/274 (59%), Positives = 196/274 (71%), Gaps = 8/274 (2%)
 Frame = -2

Query: 1846 LRLSATMSPALSFALKHLFSFSFSPHICKPKTLLFPHILNSRTAVSPPSRPLNSSNFRPL 1667
            LR+++T+ P L   L           +  PK LL P          PP         RP 
Sbjct: 2    LRVASTLRPLLRSTL-----------LSGPKPLLCPR--------HPP---------RPF 33

Query: 1666 CSISNSDNVVEKVQTSI------DPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLA 1505
             ++S+S  V     +++      +   + C    R+PL+VAVLLSGGVDSSVALRLLH A
Sbjct: 34   RALSSSSPVAASPPSALGVGDIREEHLVRCAAAGRAPLRVAVLLSGGVDSSVALRLLHAA 93

Query: 1504 GHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSY 1325
            GHSCTAFYLKIWFQEDF NFWSECPW+DDLKYA+AVCD++DVPLEV HL+DEYWN+VVS+
Sbjct: 94   GHSCTAFYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVAHLSDEYWNHVVSH 153

Query: 1324 IIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFHAS--TWDEPSLL 1151
            II EYR GRTPNPDVLCNTRIKFGAF++A+D++GFD+IASGHYA V H S    + PS+L
Sbjct: 154  IINEYRNGRTPNPDVLCNTRIKFGAFLEAIDNLGFDYIASGHYAHVVHPSIENVEGPSVL 213

Query: 1150 ELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            +LS+D VKDQTYFLSHLSQ QLRRL+FPLGCI K
Sbjct: 214  QLSKDKVKDQTYFLSHLSQPQLRRLLFPLGCITK 247


>ref|XP_006649485.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Oryza
            brachyantha]
          Length = 489

 Score =  341 bits (874), Expect(2) = e-178
 Identities = 155/218 (71%), Positives = 188/218 (86%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQDRKDSQGICFLGK+KFSEFV RHIGE EG++LEAE+GD+LG HRG
Sbjct: 256  DEVRRLAAQMDLPNQDRKDSQGICFLGKVKFSEFVERHIGEMEGILLEAETGDYLGIHRG 315

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 316  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSLDKRRRTFRVGSLNWFSNSGP 375

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               +QL+CKVRH P F+ CT++ E + + + I VVHLS+DDQGLAAGQ+A FY    C+G
Sbjct: 376  INNEQLKCKVRHSPEFHHCTVTQEQTSENRVILVVHLSEDDQGLAAGQFAAFYRDNLCLG 435

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVC++ALE+A++ED+++LGKPVKIM
Sbjct: 436  SGIILDSWDEMNFPVCARALEIAKMEDKSRLGKPVKIM 473



 Score =  313 bits (801), Expect(2) = e-178
 Identities = 154/221 (69%), Positives = 182/221 (82%), Gaps = 2/221 (0%)
 Frame = -2

Query: 1705 PSRPLNSSNFRPLCSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVDSSVA 1526
            P R ++SS   PL S S + ++ + V    D R L C    R+PL+VAVL+SGGVDSSVA
Sbjct: 37   PFRAISSSAPPPLASPSGARDLGD-VDVGED-RLLRCVEAGRAPLRVAVLVSGGVDSSVA 94

Query: 1525 LRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEY 1346
            LRLLH AGH CTAFYLKIWFQEDF NFWSECPW+DDLKY +AVCD+++VPLEVVHL+DEY
Sbjct: 95   LRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDDDLKYVQAVCDKINVPLEVVHLSDEY 154

Query: 1345 WNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFHAS--T 1172
            WN+VVS+II EYR G TPNPDVLCNTRIKFGAF++A+++MGFD+IASGHYA V H S   
Sbjct: 155  WNHVVSHIINEYRSGHTPNPDVLCNTRIKFGAFLEAIENMGFDYIASGHYAHVVHPSLDN 214

Query: 1171 WDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
             + PS+L+LS+D VKDQTYFLSHLSQTQLRRL+FPLGCI K
Sbjct: 215  VEGPSVLQLSKDKVKDQTYFLSHLSQTQLRRLLFPLGCITK 255


>ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [Sorghum bicolor]
            gi|241922267|gb|EER95411.1| hypothetical protein
            SORBIDRAFT_01g045520 [Sorghum bicolor]
          Length = 489

 Score =  340 bits (871), Expect(2) = e-178
 Identities = 156/218 (71%), Positives = 186/218 (85%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE EG+ILEAESGD+LGKHRG
Sbjct: 255  DEVRRLATQMGLPNQGRKDSQGICFLGKVKFSEFVERHIGEMEGIILEAESGDYLGKHRG 314

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W   S P
Sbjct: 315  FWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDSSGP 374

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               +QL+CKVRH P F+ CTL  E +E+ ++  VVHLS+DDQGLAAGQ+A FY   +C+G
Sbjct: 375  GNNEQLKCKVRHSPEFHDCTLIKEHTEENEDALVVHLSEDDQGLAAGQFAAFYSEHSCLG 434

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD   FPVCS+ALE+A+LED++ LGKPV+I+
Sbjct: 435  SGIILDSWDKMSFPVCSRALEIAKLEDKSSLGKPVRIL 472



 Score =  313 bits (803), Expect(2) = e-178
 Identities = 152/225 (67%), Positives = 181/225 (80%), Gaps = 2/225 (0%)
 Frame = -2

Query: 1717 AVSPPSRPLNSSNFRPLCSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVD 1538
            A+S P+    +S   P  S+   D  +E+  T        C    R+PL+VAVL+SGGVD
Sbjct: 39   ALSSPAAAAAAS--APSASVKGEDVALEEHLTR-------CAAAGRAPLRVAVLVSGGVD 89

Query: 1537 SSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHL 1358
            SSVALRLLH AGH CTAFYLKIWFQEDF NFWSECPW++DLKYA+AVCD++DVPLEVVHL
Sbjct: 90   SSVALRLLHAAGHHCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQAVCDKIDVPLEVVHL 149

Query: 1357 TDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFHA 1178
            +DEYWN VVS++I EYRCGRTPNPDVLCNTRIKFGAF++A++++GFD+IASGHYA V H 
Sbjct: 150  SDEYWNYVVSHMINEYRCGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHP 209

Query: 1177 ST--WDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            ST   + PS+L+LS+D VKDQTYFLSHLSQ QLRRL+FPLGCI K
Sbjct: 210  STENTEAPSILQLSKDKVKDQTYFLSHLSQPQLRRLLFPLGCITK 254


>gb|EEC74610.1| hypothetical protein OsI_10221 [Oryza sativa Indica Group]
          Length = 488

 Score =  335 bits (860), Expect(2) = e-177
 Identities = 154/218 (70%), Positives = 186/218 (85%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQDRKDSQGICFLGK+KFSEFV R IGE EGV+LEAE+GD+LG HRG
Sbjct: 255  DEVRRLAAQMDLPNQDRKDSQGICFLGKVKFSEFVERQIGEMEGVLLEAETGDYLGTHRG 314

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 315  FWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSNSGP 374

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
            +  +QL+CKVRH P F+ CT++ E + +   I VV LS+DDQGLAAGQ+A FY    C+G
Sbjct: 375  TNNEQLKCKVRHSPEFHDCTVTQEQTSENGVILVVRLSEDDQGLAAGQFAAFYRDNLCLG 434

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVC++ALE+AR+ED+++LGKPVKIM
Sbjct: 435  SGIILDSWDEMNFPVCARALEIARMEDKSRLGKPVKIM 472



 Score =  316 bits (810), Expect(2) = e-177
 Identities = 148/191 (77%), Positives = 169/191 (88%), Gaps = 2/191 (1%)
 Frame = -2

Query: 1615 DPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSE 1436
            D R L C    R+PL+VAVL+SGGVDSSVALRLLH AGH CTAFYLKIWFQEDF NFWSE
Sbjct: 64   DERLLRCAAAGRAPLRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSE 123

Query: 1435 CPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKF 1256
            CPW+DDLKYA+AVCD++DVPLEVVHL+DEYWN+VVS+II EYR GRTPNPDVLCNTRIKF
Sbjct: 124  CPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHIINEYRSGRTPNPDVLCNTRIKF 183

Query: 1255 GAFMDAVDDMGFDFIASGHYAKVFHASTWD--EPSLLELSRDMVKDQTYFLSHLSQTQLR 1082
            GAF++A++++GFD+IASGHYA V H S  D   PS+L+LS+D VKDQTYFLSHLSQTQLR
Sbjct: 184  GAFLEAIENLGFDYIASGHYAHVVHPSPDDVEGPSVLQLSKDKVKDQTYFLSHLSQTQLR 243

Query: 1081 RLIFPLGCIPK 1049
            RL+FPLGCI K
Sbjct: 244  RLLFPLGCITK 254


>ref|NP_001049129.1| Os03g0174600 [Oryza sativa Japonica Group]
            gi|108706458|gb|ABF94253.1| tRNA methyl transferase
            family protein, expressed [Oryza sativa Japonica Group]
            gi|113547600|dbj|BAF11043.1| Os03g0174600 [Oryza sativa
            Japonica Group] gi|215737060|dbj|BAG95989.1| unnamed
            protein product [Oryza sativa Japonica Group]
          Length = 488

 Score =  332 bits (851), Expect(2) = e-176
 Identities = 153/218 (70%), Positives = 185/218 (84%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQDRKDSQGICFLGK+KFSEFV R IGE EGV+LEAE+GD+LG HRG
Sbjct: 255  DEVRRLAAQMDLPNQDRKDSQGICFLGKVKFSEFVERQIGEMEGVLLEAETGDYLGTHRG 314

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEK V+NNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 315  FWFYTIGQRQGLRLPGGPWYVVEKGVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSNSGP 374

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
            +  +QL+CKVRH P F+ CT++ E + +   I VV LS+DDQGLAAGQ+A FY    C+G
Sbjct: 375  TNNEQLKCKVRHSPEFHDCTVTQEQTSEHGVILVVRLSEDDQGLAAGQFAAFYRDNLCLG 434

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVC++ALE+AR+ED+++LGKPVKIM
Sbjct: 435  SGIILDSWDEMNFPVCARALEIARMEDKSRLGKPVKIM 472



 Score =  316 bits (810), Expect(2) = e-176
 Identities = 148/191 (77%), Positives = 169/191 (88%), Gaps = 2/191 (1%)
 Frame = -2

Query: 1615 DPRYLSCCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSE 1436
            D R L C    R+PL+VAVL+SGGVDSSVALRLLH AGH CTAFYLKIWFQEDF NFWSE
Sbjct: 64   DERLLRCAAAGRAPLRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSE 123

Query: 1435 CPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKF 1256
            CPW+DDLKYA+AVCD++DVPLEVVHL+DEYWN+VVS+II EYR GRTPNPDVLCNTRIKF
Sbjct: 124  CPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHIINEYRSGRTPNPDVLCNTRIKF 183

Query: 1255 GAFMDAVDDMGFDFIASGHYAKVFHASTWD--EPSLLELSRDMVKDQTYFLSHLSQTQLR 1082
            GAF++A++++GFD+IASGHYA V H S  D   PS+L+LS+D VKDQTYFLSHLSQTQLR
Sbjct: 184  GAFLEAIENLGFDYIASGHYAHVVHPSPDDVEGPSVLQLSKDKVKDQTYFLSHLSQTQLR 243

Query: 1081 RLIFPLGCIPK 1049
            RL+FPLGCI K
Sbjct: 244  RLLFPLGCITK 254


>gb|ACG45558.1| tRNA methyltransferase [Zea mays]
          Length = 486

 Score =  332 bits (851), Expect(2) = e-176
 Identities = 152/218 (69%), Positives = 181/218 (83%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE EG+ILEAESGD+LG HRG
Sbjct: 252  DEVRRLATQMALPNQGRKDSQGICFLGKVKFSEFVERHIGEKEGIILEAESGDYLGIHRG 311

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W     P
Sbjct: 312  FWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDNFGP 371

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               +QL+CKVRH P F+ CTL  E SE+  +  VVHL++DDQGLAAGQ+A FY    C+G
Sbjct: 372  GNNEQLKCKVRHSPEFHDCTLVKERSEENGDALVVHLAEDDQGLAAGQFAAFYSENVCLG 431

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD   FPVCS+ALE+A+L D++ LGKP++I+
Sbjct: 432  SGIILDSWDKMSFPVCSRALEIAKLADKSSLGKPIRIV 469



 Score =  316 bits (809), Expect(2) = e-176
 Identities = 149/227 (65%), Positives = 185/227 (81%), Gaps = 10/227 (4%)
 Frame = -2

Query: 1699 RPLNSSNFRP--LCSISNS------DNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGG 1544
            +PL  S  +P   C++S+S        VV+  + ++D     C     +PL+VAVL+SGG
Sbjct: 25   KPLPRSRLQPWPFCTLSSSPAAGAPSAVVKGEEVALDKHLTRCAAAGIAPLRVAVLVSGG 84

Query: 1543 VDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVV 1364
            VDSSVALRLLH AGH CTAFYLKIWFQEDF NFWSECPW++DLKYA+AVCD++DVPLEVV
Sbjct: 85   VDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQAVCDKIDVPLEVV 144

Query: 1363 HLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVF 1184
            HL+DEYWN+VVS++I EYRCGRTPNPDVLCNTRIKFG+F++A++++GFD+IASGHYA V 
Sbjct: 145  HLSDEYWNHVVSHMINEYRCGRTPNPDVLCNTRIKFGSFLEAIENLGFDYIASGHYAHVV 204

Query: 1183 H--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            H  A   + PS+L+LS+D +KDQTYFLSHLSQ+QLRRL+FPLGCI K
Sbjct: 205  HPSAENTEAPSVLQLSKDKIKDQTYFLSHLSQSQLRRLLFPLGCITK 251


>ref|XP_004985520.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Setaria italica]
          Length = 488

 Score =  330 bits (846), Expect(2) = e-175
 Identities = 151/218 (69%), Positives = 184/218 (84%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE EG+ILEAESGD+LG HRG
Sbjct: 255  DEVRRLAAQMDLPNQGRKDSQGICFLGKVKFSEFVERHIGEMEGIILEAESGDYLGNHRG 314

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRL GGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W   S P
Sbjct: 315  FWFYTIGQRQGLRLAGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDDSGP 374

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               ++L+CKVRH P F+ CT++ E +E+  ++ VV LS+DDQGLAAGQ+A FY    C+G
Sbjct: 375  GNSERLKCKVRHSPEFHDCTVTKEHTEENGDVLVVRLSEDDQGLAAGQFAAFYREDLCLG 434

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVCS+ALE+A+LED++ LGKPV+I+
Sbjct: 435  SGIILDSWDEMSFPVCSRALEIAKLEDKSSLGKPVRII 472



 Score =  315 bits (808), Expect(2) = e-175
 Identities = 150/240 (62%), Positives = 185/240 (77%), Gaps = 11/240 (4%)
 Frame = -2

Query: 1735 ILNSRTAVSPPSRPLNSSNFRPLCSISNSDNVVEKVQTSID---------PRYLSCCMPE 1583
            +L  R  ++P +   +    RP C++S+S         S++              C   +
Sbjct: 15   LLRRRPLLNPKALLRSRLPPRPFCALSSSAPPAAAAAPSVEGAGGAVAVEEHLTRCAAAD 74

Query: 1582 RSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAK 1403
            R+PL+VAVL+SGGVDSSVALRLLH AGH CTAFYLKIWFQEDF NFWSECPW++DLKYA+
Sbjct: 75   RAPLRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQ 134

Query: 1402 AVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMG 1223
             VCD++DVPLEVVHL+DEYWN+VVS+II EYRCGRTPNPDVLCNTRIKFGAF++A++++G
Sbjct: 135  VVCDKIDVPLEVVHLSDEYWNHVVSHIINEYRCGRTPNPDVLCNTRIKFGAFLEAIENLG 194

Query: 1222 FDFIASGHYAKVFHAS--TWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            FD+IASGHYA V H S    + PS+L+LS+D +KDQTYFLSHLSQ QLRRL+FPLGCI K
Sbjct: 195  FDYIASGHYAHVVHPSVENTEAPSVLQLSKDKIKDQTYFLSHLSQAQLRRLLFPLGCIKK 254


>ref|NP_001131207.1| uncharacterized protein LOC100192515 [Zea mays]
            gi|194690872|gb|ACF79520.1| unknown [Zea mays]
            gi|414865088|tpg|DAA43645.1| TPA: tRNA methyltransferase
            [Zea mays]
          Length = 486

 Score =  331 bits (849), Expect(2) = e-175
 Identities = 152/218 (69%), Positives = 181/218 (83%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR+LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE EG+ILEAESGD+LG HRG
Sbjct: 252  DEVRRLATQMGLPNQGRKDSQGICFLGKVKFSEFVERHIGEKEGIILEAESGDYLGIHRG 311

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRLPGGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W     P
Sbjct: 312  FWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDNCGP 371

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
               +QL+CKVRH P F+ CTL  E SE+  +  VVHL++DDQGLAAGQ+A FY    C+G
Sbjct: 372  GNNEQLKCKVRHSPEFHDCTLVKERSEENGDALVVHLAEDDQGLAAGQFAAFYSENVCLG 431

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD   FPVCS+ALE+A+L D++ LGKP++I+
Sbjct: 432  SGIILDSWDKMSFPVCSRALEIAKLADKSSLGKPIRIV 469



 Score =  314 bits (805), Expect(2) = e-175
 Identities = 148/227 (65%), Positives = 184/227 (81%), Gaps = 10/227 (4%)
 Frame = -2

Query: 1699 RPLNSSNFRP--LCSISNS------DNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGG 1544
            +PL  S  +P   C++S+S         V+  + ++D     C     +PL+VAVL+SGG
Sbjct: 25   KPLPRSRLQPWPFCTLSSSPAAGAPSAAVKGEEVALDKHLTRCAAAGIAPLRVAVLVSGG 84

Query: 1543 VDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVV 1364
            VDSSVALRLLH AGH CTAFYLKIWFQEDF NFWSECPW++DLKYA+AVCD++DVPLEVV
Sbjct: 85   VDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQAVCDKIDVPLEVV 144

Query: 1363 HLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVF 1184
            HL+DEYWN+VVS++I EYRCGRTPNPDVLCNTRIKFG+F++A++++GFD+IASGHYA V 
Sbjct: 145  HLSDEYWNHVVSHMINEYRCGRTPNPDVLCNTRIKFGSFLEAIENLGFDYIASGHYAHVV 204

Query: 1183 H--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
            H  A   + PS+L+LS+D +KDQTYFLSHLSQ+QLRRL+FPLGCI K
Sbjct: 205  HPSAENTEAPSVLQLSKDKIKDQTYFLSHLSQSQLRRLLFPLGCITK 251


>gb|EMT11416.1| tRNA-specific 2-thiouridylase mnmA [Aegilops tauschii]
          Length = 552

 Score =  329 bits (844), Expect(2) = e-173
 Identities = 149/218 (68%), Positives = 185/218 (84%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR LA  + LPNQ RKDSQGICFLGK+KFSEFV RHIGE +G++LEAE+GD+LG HRG
Sbjct: 252  DEVRSLAVQMDLPNQARKDSQGICFLGKVKFSEFVQRHIGEMKGILLEAETGDYLGMHRG 311

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRL GGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 312  FWFYTIGQRQGLRLSGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGP 371

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEKGKEIGVVHLSQDDQGLAAGQYATFYDGTTCIG 504
            S  ++L+CKVRH P F+ CT++ E +E+  ++  V LS+DDQGLAAGQ+A FY  + C+G
Sbjct: 372  SDNERLKCKVRHSPDFHDCTVTKEQTEENGDVLAVRLSEDDQGLAAGQFAAFYRDSACLG 431

Query: 503  SGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
            SG+IL+SWD+  FPVC+KALE AR+ED++K+GKP++IM
Sbjct: 432  SGIILDSWDEMSFPVCAKALETARMEDKSKMGKPIRIM 469



 Score =  309 bits (791), Expect(2) = e-173
 Identities = 152/225 (67%), Positives = 178/225 (79%), Gaps = 2/225 (0%)
 Frame = -2

Query: 1717 AVSPPSRPLNSSNFRPLCSISNSDNVVEKVQTSIDPRYLSCCMPERSPLKVAVLLSGGVD 1538
            A SPPS P            S +   VE  +  +    + C    R+PL+VAVLLSGGVD
Sbjct: 43   AASPPSAP------------SPAPGAVEFREEHL----VRCAAARRAPLRVAVLLSGGVD 86

Query: 1537 SSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHL 1358
            SSVALRLLH AGHSCTAFYLKIWFQEDF NFWSECPW+DDLKYA+AVCD++DVPLEVVHL
Sbjct: 87   SSVALRLLHAAGHSCTAFYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVVHL 146

Query: 1357 TDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAVDDMGFDFIASGHYAKVFHA 1178
            +DEYWN+VVS++I EYR GRTPNPDVLCNTRIKFGAF++A++++GFD+IASGHYA V H 
Sbjct: 147  SDEYWNHVVSHMINEYRNGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHP 206

Query: 1177 --STWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPLGCIPK 1049
                 + PS+L+LS+D VKDQTYFLSHLSQ QLRRL+FPLGCI K
Sbjct: 207  PFENAEGPSVLQLSKDEVKDQTYFLSHLSQPQLRRLLFPLGCITK 251


>dbj|BAK01046.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 491

 Score =  323 bits (829), Expect(2) = e-171
 Identities = 151/224 (67%), Positives = 185/224 (82%), Gaps = 6/224 (2%)
 Frame = -1

Query: 1043 DEVRKLAKTLLLPNQDRKDSQGICFLGKIKFSEFVARHIGEAEGVILEAESGDFLGKHRG 864
            DEVR LA  + LPNQ RKDSQGICFLGK+KFSEFV  HIGE EG++LEAE+GD+LG HRG
Sbjct: 252  DEVRSLAVQMNLPNQARKDSQGICFLGKVKFSEFVQAHIGEIEGILLEAETGDYLGTHRG 311

Query: 863  FWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSVDKRRRSFRIGSFKWLSGSTP 684
            FWFYTIGQRQGLRL GGPWYVVEKDV+NNVVFVSRNY+S+DKRRR+FR+GS  W S S P
Sbjct: 312  FWFYTIGQRQGLRLSGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGP 371

Query: 683  SKIDQLQCKVRHGPGFYSCTLSMETSEK------GKEIGVVHLSQDDQGLAAGQYATFYD 522
            +  ++L+CKVRH P F++CT++ E +E+        ++  V LS+DDQGLAAGQ+A FY 
Sbjct: 372  ADNERLKCKVRHSPDFHNCTVTREQTEELVVNSNNGDVLAVRLSEDDQGLAAGQFAAFYR 431

Query: 521  GTTCIGSGVILESWDDKGFPVCSKALELARLEDETKLGKPVKIM 390
             + C+GSG+IL+SWD+  FPVC+KALE ARLED++KLGKPV+IM
Sbjct: 432  DSACLGSGIILDSWDEMSFPVCAKALETARLEDKSKLGKPVRIM 475



 Score =  309 bits (791), Expect(2) = e-171
 Identities = 143/185 (77%), Positives = 166/185 (89%), Gaps = 2/185 (1%)
 Frame = -2

Query: 1597 CCMPERSPLKVAVLLSGGVDSSVALRLLHLAGHSCTAFYLKIWFQEDFENFWSECPWEDD 1418
            C    R+PL+VAVLLSGGVDSSVALRLLH AGHSCTAFYLKIWFQEDF NFWSECPW+DD
Sbjct: 67   CAAAGRAPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFRNFWSECPWDDD 126

Query: 1417 LKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDA 1238
            LKYA+AVCD++DVPLEVVHL+DEYWN+VVS++I EYR GRTPNPDVLCNTRIKFGAF++A
Sbjct: 127  LKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHMISEYRNGRTPNPDVLCNTRIKFGAFLEA 186

Query: 1237 VDDMGFDFIASGHYAKVFH--ASTWDEPSLLELSRDMVKDQTYFLSHLSQTQLRRLIFPL 1064
            ++++GFD+IASGHYA V H      + PS+L+LS+D VKDQTYFLSHLSQ+QLRRL+FPL
Sbjct: 187  IENLGFDYIASGHYAHVVHPPVENAEGPSVLQLSKDEVKDQTYFLSHLSQSQLRRLLFPL 246

Query: 1063 GCIPK 1049
            GCI K
Sbjct: 247  GCITK 251