BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Phellodendron21_contig00034524
         (324 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KDO75685.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]    215   3e-65
KDO75684.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]    215   4e-65
XP_019249835.1 PREDICTED: probable serine/threonine-protein kina...   210   6e-65
XP_009772339.1 PREDICTED: probable serine/threonine-protein kina...   210   6e-65
XP_009772338.1 PREDICTED: probable serine/threonine-protein kina...   210   7e-65
KDO75682.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]    215   7e-65
XP_006467913.1 PREDICTED: probable serine/threonine-protein kina...   215   7e-65
XP_006449194.1 hypothetical protein CICLE_v10014596mg [Citrus cl...   215   7e-65
KDO75683.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]    215   1e-64
XP_009772336.1 PREDICTED: probable serine/threonine-protein kina...   210   3e-64
EEF37718.1 ATATH13, putative [Ricinus communis]                       213   3e-64
XP_020088770.1 probable serine/threonine-protein kinase abkC iso...   209   4e-64
XP_008440290.1 PREDICTED: probable serine/threonine-protein kina...   213   5e-64
XP_015578186.1 PREDICTED: LOW QUALITY PROTEIN: probable serine/t...   213   5e-64
XP_015066176.1 PREDICTED: probable serine/threonine-protein kina...   212   1e-63
XP_006340706.1 PREDICTED: probable serine/threonine-protein kina...   212   1e-63
XP_004232465.1 PREDICTED: probable serine/threonine-protein kina...   212   1e-63
XP_010927618.1 PREDICTED: probable serine/threonine-protein kina...   212   1e-63
XP_018675454.1 PREDICTED: probable serine/threonine-protein kina...   207   1e-63
XP_019177473.1 PREDICTED: probable serine/threonine-protein kina...   212   1e-63

>KDO75685.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]
          Length = 581

 Score =  215 bits (548), Expect = 3e-65
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 354 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 413

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 414 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 460


>KDO75684.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]
          Length = 601

 Score =  215 bits (548), Expect = 4e-65
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 354 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 413

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 414 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 460


>XP_019249835.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X2
           [Nicotiana attenuata]
          Length = 416

 Score =  210 bits (535), Expect = 6e-65
 Identities = 102/107 (95%), Positives = 104/107 (97%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRF YNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 140 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFTYNFRRWKDVSFPKPVYPLVHPAVLVETF 199

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 200 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 246


>XP_009772339.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X4
           [Nicotiana sylvestris]
          Length = 416

 Score =  210 bits (535), Expect = 6e-65
 Identities = 102/107 (95%), Positives = 104/107 (97%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRF YNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 140 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFTYNFRRWKDVSFPKPVYPLVHPAVLVETF 199

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 200 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 246


>XP_009772338.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X3
           [Nicotiana sylvestris]
          Length = 419

 Score =  210 bits (535), Expect = 7e-65
 Identities = 102/107 (95%), Positives = 104/107 (97%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRF YNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 143 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFTYNFRRWKDVSFPKPVYPLVHPAVLVETF 202

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 203 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 249


>KDO75682.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]
          Length = 630

 Score =  215 bits (548), Expect = 7e-65
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 354 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 413

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 414 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 460


>XP_006467913.1 PREDICTED: probable serine/threonine-protein kinase abkC [Citrus
           sinensis]
          Length = 630

 Score =  215 bits (548), Expect = 7e-65
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 354 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 413

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 414 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 460


>XP_006449194.1 hypothetical protein CICLE_v10014596mg [Citrus clementina]
           XP_006449195.1 hypothetical protein CICLE_v10014596mg
           [Citrus clementina] ESR62434.1 hypothetical protein
           CICLE_v10014596mg [Citrus clementina] ESR62435.1
           hypothetical protein CICLE_v10014596mg [Citrus
           clementina]
          Length = 630

 Score =  215 bits (548), Expect = 7e-65
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 354 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 413

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 414 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 460


>KDO75683.1 hypothetical protein CISIN_1g006322mg [Citrus sinensis]
          Length = 650

 Score =  215 bits (548), Expect = 1e-64
 Identities = 104/107 (97%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 374 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 433

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VSRYVDEL+GHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 434 EQGECVSRYVDELQGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 480


>XP_009772336.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X2
           [Nicotiana sylvestris]
          Length = 484

 Score =  210 bits (535), Expect = 3e-64
 Identities = 102/107 (95%), Positives = 104/107 (97%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRF YNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 208 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFTYNFRRWKDVSFPKPVYPLVHPAVLVETF 267

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 268 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 314


>EEF37718.1 ATATH13, putative [Ricinus communis]
          Length = 603

 Score =  213 bits (542), Expect = 3e-64
 Identities = 102/107 (95%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 327 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 386

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGESVS +VDELEGH+R+KSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 387 EQGESVSHFVDELEGHDRIKSALAHIGTHALLKMLLVDNFIHADMHP 433


>XP_020088770.1 probable serine/threonine-protein kinase abkC isoform X5 [Ananas
           comosus]
          Length = 465

 Score =  209 bits (533), Expect = 4e-64
 Identities = 100/107 (93%), Positives = 105/107 (98%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRW+DVSFPKP+YPLVHPAVLVETY
Sbjct: 193 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWRDVSFPKPLYPLVHPAVLVETY 252

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGESVS YVD LEGH+RLKSALAHIGTHALLKMLLVDNF+HADMHP
Sbjct: 253 EQGESVSYYVDALEGHDRLKSALAHIGTHALLKMLLVDNFVHADMHP 299


>XP_008440290.1 PREDICTED: probable serine/threonine-protein kinase abkC [Cucumis
           melo] XP_008440291.1 PREDICTED: probable
           serine/threonine-protein kinase abkC [Cucumis melo]
          Length = 624

 Score =  213 bits (542), Expect = 5e-64
 Identities = 103/107 (96%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 348 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 407

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGESVS YVDELEG+ERLKS+LAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 408 EQGESVSHYVDELEGYERLKSSLAHIGTHALLKMLLVDNFIHADMHP 454


>XP_015578186.1 PREDICTED: LOW QUALITY PROTEIN: probable serine/threonine-protein
           kinase abkC [Ricinus communis]
          Length = 625

 Score =  213 bits (542), Expect = 5e-64
 Identities = 102/107 (95%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY
Sbjct: 349 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 408

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGESVS +VDELEGH+R+KSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 409 EQGESVSHFVDELEGHDRIKSALAHIGTHALLKMLLVDNFIHADMHP 455


>XP_015066176.1 PREDICTED: probable serine/threonine-protein kinase abkC [Solanum
           pennellii]
          Length = 624

 Score =  212 bits (540), Expect = 1e-63
 Identities = 103/107 (96%), Positives = 105/107 (98%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 348 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETF 407

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 408 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 454


>XP_006340706.1 PREDICTED: probable serine/threonine-protein kinase abkC [Solanum
           tuberosum]
          Length = 624

 Score =  212 bits (540), Expect = 1e-63
 Identities = 103/107 (96%), Positives = 105/107 (98%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 348 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETF 407

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 408 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 454


>XP_004232465.1 PREDICTED: probable serine/threonine-protein kinase abkC [Solanum
           lycopersicum]
          Length = 624

 Score =  212 bits (540), Expect = 1e-63
 Identities = 103/107 (96%), Positives = 105/107 (98%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 348 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETF 407

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGE VS YVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 408 EQGECVSYYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 454


>XP_010927618.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X2
           [Elaeis guineensis]
          Length = 610

 Score =  212 bits (539), Expect = 1e-63
 Identities = 101/107 (94%), Positives = 105/107 (98%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKP+YPLVHPAVLVETY
Sbjct: 355 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPLYPLVHPAVLVETY 414

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           E GESVS YVDELEGH+RLKSALAHIGTHALLKMLLVDNF+HADMHP
Sbjct: 415 EHGESVSHYVDELEGHDRLKSALAHIGTHALLKMLLVDNFVHADMHP 461


>XP_018675454.1 PREDICTED: probable serine/threonine-protein kinase abkC isoform X4
           [Musa acuminata subsp. malaccensis]
          Length = 436

 Score =  207 bits (528), Expect = 1e-63
 Identities = 99/107 (92%), Positives = 103/107 (96%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDL+REAAHL+RFIYNFRRWKDVSFPKP+YP VHPAVLVETY
Sbjct: 161 WLRLDESVQQFAVFMMSQVDLSREAAHLNRFIYNFRRWKDVSFPKPLYPFVHPAVLVETY 220

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           E GESVS YVDELEGH RLKSALAHIGTHALLKMLLVDNFIHADMHP
Sbjct: 221 EHGESVSHYVDELEGHSRLKSALAHIGTHALLKMLLVDNFIHADMHP 267


>XP_019177473.1 PREDICTED: probable serine/threonine-protein kinase abkC [Ipomoea
           nil]
          Length = 624

 Score =  212 bits (539), Expect = 1e-63
 Identities = 101/107 (94%), Positives = 106/107 (99%)
 Frame = -3

Query: 322 WLRLDESVRQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETY 143
           WLRLDESV+QFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVET+
Sbjct: 348 WLRLDESVQQFAVFMMSQVDLAREAAHLSRFIYNFRRWKDVSFPKPVYPLVHPAVLVETF 407

Query: 142 EQGESVSRYVDELEGHERLKSALAHIGTHALLKMLLVDNFIHADMHP 2
           EQGESVS YVDEL+GHER+KSALAHIGTHALLKMLLVDNF+HADMHP
Sbjct: 408 EQGESVSYYVDELQGHERIKSALAHIGTHALLKMLLVDNFVHADMHP 454