BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Phellodendron21_contig00024807
         (1829 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_007413400.1 hypothetical protein MELLADRAFT_75337 [Melampsora...   764   0.0  
KNF00316.1 hypothetical protein PSTG_06489 [Puccinia striiformis...   656   0.0  
XP_003307138.1 omega-6 fatty acid desaturase (delta-12 desaturas...   649   0.0  
OAV91895.1 hypothetical protein PTTG_02638 [Puccinia triticina 1...   648   0.0  
KNZ59565.1 omega-6 fatty acid desaturase (Delta-12 desaturase) [...   647   0.0  
OAV91896.1 hypothetical protein PTTG_02637 [Puccinia triticina 1...   603   0.0  
XP_007403496.1 hypothetical protein MELLADRAFT_70376 [Melampsora...   583   0.0  
OAV91880.1 hypothetical protein PTTG_02647 [Puccinia triticina 1...   571   0.0  
KNZ62768.1 omega-6 fatty acid desaturase (Delta-12 desaturase) [...   559   0.0  
XP_003307171.1 omega-6 fatty acid desaturase (delta-12 desaturas...   558   0.0  
KNF00291.1 hypothetical protein PSTG_06464 [Puccinia striiformis...   550   0.0  
KNF00278.1 hypothetical protein PSTG_06449 [Puccinia striiformis...   528   0.0  
XP_014566653.1 hypothetical protein L969DRAFT_95919 [Mixia osmun...   489   e-166
KNE92012.1 hypothetical protein PSTG_14600 [Puccinia striiformis...   467   e-157
KDE03817.1 omega-6 fatty acid desaturase (delta-12 desaturase) [...   463   e-155
CBQ70450.1 related to Delta-12 fatty acid desaturase [Sporisoriu...   461   e-154
XP_011387393.1 hypothetical protein UMAG_11270 [Ustilago maydis ...   457   e-152
XP_012190813.1 likely delta-12 fatty acid desaturase [Pseudozyma...   455   e-152
CCF54793.1 related to Delta-12 fatty acid desaturase [Ustilago h...   452   e-151
CDW97138.1 hypothetical protein [Sporisorium scitamineum] CDU246...   452   e-150

>XP_007413400.1 hypothetical protein MELLADRAFT_75337 [Melampsora larici-populina
            98AG31] EGG03265.1 hypothetical protein MELLADRAFT_75337
            [Melampsora larici-populina 98AG31]
          Length = 465

 Score =  764 bits (1972), Expect = 0.0
 Identities = 361/462 (78%), Positives = 395/462 (85%), Gaps = 1/462 (0%)
 Frame = -3

Query: 1596 MSSSPVFSNLDAPPNTLT-RRSGNASPKVIHLAXXXXXXXXXXXXXSHLPTFEPPPFTIK 1420
            MSSS     +D+   T   ++S    PK+IH+                +P FE PPFTIK
Sbjct: 1    MSSSTGIDRIDSTQLTHRFQKSSEQQPKIIHI-DSTRSSTPDSLDQDEIPCFELPPFTIK 59

Query: 1419 ELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKILDGKLGAMAN 1240
            E+L +IPSHCFDRSL RS SYV+RD +FTA+LVYAATFID  F S+ G ILDG+LGAMAN
Sbjct: 60   EILSSIPSHCFDRSLFRSLSYVLRDFSFTALLVYAATFIDGAFNSKNGSILDGQLGAMAN 119

Query: 1239 FAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLVPFHSWRITHA 1060
            F  W AYGYALGLV+TG+WVLAHECGHQSFS SKTINYW GW+LHSALLVPFHSWRITHA
Sbjct: 120  FGAWAAYGYALGLVFTGIWVLAHECGHQSFSSSKTINYWVGWILHSALLVPFHSWRITHA 179

Query: 1059 QHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERVDDLLEDAPLWA 880
            QHHASTSH+++DQAFVPYTRSQLNLPPL  DAS+REI+G +AP+FYERVDDLLEDAPLWA
Sbjct: 180  QHHASTSHLNRDQAFVPYTRSQLNLPPLPKDASKREIEGTLAPSFYERVDDLLEDAPLWA 239

Query: 879  LSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIG 700
            L +L VHQS+GFAAYL+RNASGQKHYPPYTNHFNPWA+MY DRHRM IVYSDIGIAITIG
Sbjct: 240  LWKLIVHQSIGFAAYLVRNASGQKHYPPYTNHFNPWAIMYDDRHRMHIVYSDIGIAITIG 299

Query: 699  SLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATM 520
            SLV  GMKTD L+  KYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATM
Sbjct: 300  SLVFFGMKTDFLSAFKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATM 359

Query: 519  DRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVF 340
            DR IHGFFTHGLAETHVAHHLCSKIPHYHAW+AT+ALK KLG HYQST ENYW+SLWK F
Sbjct: 360  DRKIHGFFTHGLAETHVAHHLCSKIPHYHAWEATDALKAKLGPHYQSTDENYWVSLWKAF 419

Query: 339  RHCRFVEDEGDVVFYKNAKGQAAIKLKSSNGSASDSGIEVDQ 214
            RHCRFVEDEGDVVFYKNAKGQA I+LK S+GS SDSGIEVDQ
Sbjct: 420  RHCRFVEDEGDVVFYKNAKGQALIRLKESDGSPSDSGIEVDQ 461


>KNF00316.1 hypothetical protein PSTG_06489 [Puccinia striiformis f. sp. tritici
            PST-78]
          Length = 472

 Score =  656 bits (1692), Expect = 0.0
 Identities = 316/475 (66%), Positives = 370/475 (77%), Gaps = 4/475 (0%)
 Frame = -3

Query: 1629 LGPTTPSQTIFMSSSPVFSNLDAPPNTLTRRSGNASPKVIHLAXXXXXXXXXXXXXSHLP 1450
            L   +  + I ++SSP  S   + P++LT                             + 
Sbjct: 14   LASRSSGKVIHLTSSPDSSTRSSSPDSLTNDKDG------------------------IA 49

Query: 1449 TFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKI 1270
            TFE P FTIKELLG+IP+HCF+RSL +S  YV+RDLAFT  L+Y    ID NF S  GK+
Sbjct: 50   TFELPEFTIKELLGSIPAHCFERSLFKSSLYVVRDLAFTLALIYLGRQIDPNFNSVDGKL 109

Query: 1269 LDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLV 1090
            + G+ GA+ NF  W  YGY +GLVWTG+WVLAHECGHQSFSPSK+IN   GWVLHSALLV
Sbjct: 110  VSGRAGAVLNFLGWAFYGYWMGLVWTGIWVLAHECGHQSFSPSKSINNAVGWVLHSALLV 169

Query: 1089 PFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERVD 910
            PFHSWRITHAQHHA+T HM +DQAFVPYTRSQL LPPL+   S++E+ G+ +P+FYERVD
Sbjct: 170  PFHSWRITHAQHHAATCHMKRDQAFVPYTRSQLGLPPLAEGCSKKEVDGSHSPSFYERVD 229

Query: 909  DLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPP-YTNHFNPWAVMYGDRHRMQIV 733
            DLLEDAPLWAL +L VHQSVGFA+YL+ NASGQKHYP  +TNHFNP+A+M+ DRHR Q+V
Sbjct: 230  DLLEDAPLWALYKLIVHQSVGFASYLMFNASGQKHYPRWWTNHFNPYAIMFDDRHRFQVV 289

Query: 732  YSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGA 553
            +SDIGIAITIG +  LG +TD LT  KYYIVPYL VHHWIVLITFLQHTDPLLPHYREGA
Sbjct: 290  WSDIGIAITIGCVTYLGKQTDFLTAFKYYIVPYLVVHHWIVLITFLQHTDPLLPHYREGA 349

Query: 552  FNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQSTT 373
            FNFQRGA++TMDRNIHGFFTHGLAETH+AHHLCSKIPHY+AW+AT+ALK KLGHHY ST 
Sbjct: 350  FNFQRGAMSTMDRNIHGFFTHGLAETHIAHHLCSKIPHYNAWEATDALKAKLGHHYSSTD 409

Query: 372  ENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIK-LKSSNGSA--SDSGIEVD 217
            ENYW SLWK FR CRFVEDEGDVVFYK+A+G+AA + +   NGSA  SDSG++VD
Sbjct: 410  ENYWYSLWKCFRQCRFVEDEGDVVFYKDARGKAARRYVPRGNGSASVSDSGVDVD 464


>XP_003307138.1 omega-6 fatty acid desaturase (delta-12 desaturase) [Puccinia
            graminis f. sp. tritici CRL 75-36-700-3] EFP74132.1
            omega-6 fatty acid desaturase (delta-12 desaturase)
            [Puccinia graminis f. sp. tritici CRL 75-36-700-3]
          Length = 472

 Score =  649 bits (1675), Expect = 0.0
 Identities = 308/470 (65%), Positives = 367/470 (78%), Gaps = 4/470 (0%)
 Frame = -3

Query: 1614 PSQTIFMSSSPVFSNLDAPPNTLTRRSGNASPKVIHLAXXXXXXXXXXXXXSHLPTFEPP 1435
            P + I ++SSP  S   + P++LT                             + TFE P
Sbjct: 19   PGKVIHLTSSPSSSTRSSSPDSLTNEKDG------------------------IATFELP 54

Query: 1434 PFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKILDGKL 1255
             FTIKELLG+IP+HCF+RSL +S  YV+RDL+FT  L+Y    ID NF S  G++++G+ 
Sbjct: 55   EFTIKELLGSIPAHCFERSLFKSSLYVLRDLSFTLALIYLGRQIDPNFNSVDGQLVNGRP 114

Query: 1254 GAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLVPFHSW 1075
            GA+ NF  W  YGY +GLVWTG+WVLAHECGHQSFSPSK+IN   GWVLHSALLVPFHSW
Sbjct: 115  GAILNFLAWAFYGYWMGLVWTGIWVLAHECGHQSFSPSKSINNAVGWVLHSALLVPFHSW 174

Query: 1074 RITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERVDDLLED 895
            RITHAQHHA+T HM +DQAFVPYTRSQL LPPL+   S++E++G+  P+FYERVDDLLED
Sbjct: 175  RITHAQHHAATCHMKRDQAFVPYTRSQLGLPPLAEGCSKKEVEGSHPPSFYERVDDLLED 234

Query: 894  APLWALSQLFVHQSVGFAAYLLRNASGQKHYPP-YTNHFNPWAVMYGDRHRMQIVYSDIG 718
            APLWAL +L +HQS+GFA+YLL NASGQKHYP  +TNHFNP+A+M+ +RHR Q+V+SD+G
Sbjct: 235  APLWALYKLIIHQSIGFASYLLINASGQKHYPRWWTNHFNPYAIMFDERHRSQVVWSDLG 294

Query: 717  IAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQR 538
            IAITI  L  LG +TD LTV KYYIVPYL VHHWIVLITFLQHTDPLLPHYREGAFNFQR
Sbjct: 295  IAITISCLTYLGKQTDFLTVFKYYIVPYLVVHHWIVLITFLQHTDPLLPHYREGAFNFQR 354

Query: 537  GALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQSTTENYWL 358
            GA++TMDRNIHGFFTHGLAETHVAHHLCSKIPHY+AW+AT+ALK KLG HY ST EN+W 
Sbjct: 355  GAMSTMDRNIHGFFTHGLAETHVAHHLCSKIPHYNAWEATDALKAKLGDHYSSTDENFWY 414

Query: 357  SLWKVFRHCRFVEDEGDVVFYKNAKGQAA---IKLKSSNGSASDSGIEVD 217
            SLWK FR CRFVEDEGDVVFYK+A+G+AA   +   + + S SDSG++VD
Sbjct: 415  SLWKCFRQCRFVEDEGDVVFYKDARGKAARRYVPRNTGSSSVSDSGVDVD 464


>OAV91895.1 hypothetical protein PTTG_02638 [Puccinia triticina 1-1 BBBD Race 1]
          Length = 467

 Score =  648 bits (1671), Expect = 0.0
 Identities = 301/420 (71%), Positives = 351/420 (83%), Gaps = 4/420 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            + TFE P FTIKELLGAIP+HCF+RSL +S  YV+RDL+FT  L+Y    ID NF S  G
Sbjct: 43   IATFELPEFTIKELLGAIPAHCFERSLFKSSLYVVRDLSFTLALIYLGRQIDPNFNSVDG 102

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
            +++ G+ GA+ NF  W  YGY +GLVWTG+WVLAHECGHQSFSPSK+IN   GWVLHSAL
Sbjct: 103  QLVSGRPGAILNFLGWAFYGYWMGLVWTGIWVLAHECGHQSFSPSKSINNAVGWVLHSAL 162

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVPFHSWRITHAQHHA+T HM +DQAFVPYTRSQL LPPL+   S++E+ G+  P+FY+R
Sbjct: 163  LVPFHSWRITHAQHHAATCHMKRDQAFVPYTRSQLGLPPLAEGCSKKEVDGSHPPSFYDR 222

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPP-YTNHFNPWAVMYGDRHRMQ 739
            VDDLLEDAPLWAL +L +HQS+GFA+YLL NASGQKHYP  +TNHFNP+A+M+ DRHR Q
Sbjct: 223  VDDLLEDAPLWALYKLIIHQSIGFASYLLINASGQKHYPRWWTNHFNPYAIMFDDRHRSQ 282

Query: 738  IVYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYRE 559
            +V+SDIGIAIT+  +  LG +TD LTV KYYIVPYL VHHWIVLITFLQHTDPLLPHYRE
Sbjct: 283  VVWSDIGIAITLSCVTYLGKQTDFLTVFKYYIVPYLVVHHWIVLITFLQHTDPLLPHYRE 342

Query: 558  GAFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQS 379
            GAFNFQRGA++TMDRNIHGFFTHGLAETHVAHHLCSKIPHY+AW+AT+ALK KLG HY S
Sbjct: 343  GAFNFQRGAMSTMDRNIHGFFTHGLAETHVAHHLCSKIPHYNAWEATDALKAKLGEHYAS 402

Query: 378  TTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAA---IKLKSSNGSASDSGIEVDQKP 208
            T ENYW SLWK FR CRFVEDEGDVVFYK+A+G+AA   +   + + S SDSG++VD  P
Sbjct: 403  TDENYWYSLWKCFRQCRFVEDEGDVVFYKDARGKAARRYVPRNTGSSSVSDSGVDVDGSP 462


>KNZ59565.1 omega-6 fatty acid desaturase (Delta-12 desaturase) [Puccinia sorghi]
          Length = 465

 Score =  647 bits (1669), Expect = 0.0
 Identities = 302/407 (74%), Positives = 344/407 (84%), Gaps = 1/407 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            + TFE P FTIKELLG+IP+HCF+RSL +S  YVIRDL+FT  L++    ID NF S  G
Sbjct: 47   IATFELPEFTIKELLGSIPAHCFERSLFKSSLYVIRDLSFTLALIFLGRQIDPNFNSLDG 106

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
            +++ G+ GA  NF  WG YGY +GLVWTG+WVLAHECGHQSFSPSK+IN   GWVLHSAL
Sbjct: 107  QLVRGRAGAFLNFLAWGFYGYWMGLVWTGIWVLAHECGHQSFSPSKSINNAVGWVLHSAL 166

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVPFHSWRITH+QHHA+T HM++DQAFVPYTRSQL LPPL    S++E++G+ +P+FYER
Sbjct: 167  LVPFHSWRITHSQHHAATCHMNRDQAFVPYTRSQLGLPPLPEGCSKKEVEGSHSPSFYER 226

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            VDDLLEDAPLWAL +L VHQSVGFA YLL NASGQKHYP +TNHFNP+AVM+ DRHR Q+
Sbjct: 227  VDDLLEDAPLWALYKLIVHQSVGFATYLLINASGQKHYPAWTNHFNPYAVMFDDRHRSQV 286

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
            V+SD+GIAITI  L  LG +TD LTV KYYIVPYL VHHWIVLITFLQHTDPLLPHYREG
Sbjct: 287  VWSDLGIAITISCLTYLGKQTDFLTVFKYYIVPYLVVHHWIVLITFLQHTDPLLPHYREG 346

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            AFNFQRGA++TMDR IHGFFTHGLAETHV HHLCSKIPHY+AW+ATEALK KLG HY ST
Sbjct: 347  AFNFQRGAISTMDRKIHGFFTHGLAETHVTHHLCSKIPHYNAWEATEALKAKLGEHYCST 406

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIK-LKSSNGSAS 238
             ENYW SLWK FR CRFVEDEGDVVFYKNAKG+AA + +   NGS+S
Sbjct: 407  DENYWYSLWKCFRQCRFVEDEGDVVFYKNAKGKAARRYVPKDNGSSS 453


>OAV91896.1 hypothetical protein PTTG_02637 [Puccinia triticina 1-1 BBBD Race 1]
          Length = 468

 Score =  603 bits (1556), Expect = 0.0
 Identities = 290/471 (61%), Positives = 347/471 (73%), Gaps = 4/471 (0%)
 Frame = -3

Query: 1614 PSQTIFMSSSPVFSNLDAPPNTLTRRSGNASPKVIHLAXXXXXXXXXXXXXSHLPTFEPP 1435
            P + I ++SSP    + + P +LT    +                        +PTFE  
Sbjct: 19   PEKVIHLTSSPSSPTMSSSPKSLTNDEDD------------------------IPTFELS 54

Query: 1434 PFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKILDGKL 1255
             FTIKELLGAIP+HCF+RSL +S  YV+RD +FT  LV+    ID NF    G++++G+ 
Sbjct: 55   EFTIKELLGAIPAHCFERSLFKSSLYVLRDSSFTIALVFLGRQIDPNFNRVDGQLVNGRE 114

Query: 1254 GAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLVPFHSW 1075
            GA+ NF  W  YGY +GLVW G WVL HECGH +FSP  +IN   GWVLHSALLVPFHSW
Sbjct: 115  GAILNFLAWAFYGYWMGLVWMGFWVLGHECGHHAFSPYNSINLAVGWVLHSALLVPFHSW 174

Query: 1074 RITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERVDDLLED 895
            RITHAQHHA T HM ++QAFVP TRSQL LPPL+   S++E+ G+  P+FY++VDDLL D
Sbjct: 175  RITHAQHHAGTCHMKREQAFVPCTRSQLGLPPLADGCSKKEVDGSHPPSFYDKVDDLLVD 234

Query: 894  APLWALSQLFVHQSVGFAAYLLRNASGQKHYPP-YTNHFNPWAVMYGDRHRMQIVYSDIG 718
            APLWAL +L +HQS+GF  YLL NASGQKHYP  +TNH NP+A+M+G RHR Q+++SD+G
Sbjct: 235  APLWALFKLIIHQSIGFTTYLLTNASGQKHYPRWWTNHLNPYAIMFGPRHRSQVLWSDLG 294

Query: 717  IAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQR 538
            IAITI  L  LG  TD LTV KYYIVPYL VHHW+VLITFLQHTDP LPHYREGAF FQR
Sbjct: 295  IAITISCLTYLGKHTDFLTVFKYYIVPYLIVHHWMVLITFLQHTDPSLPHYREGAFTFQR 354

Query: 537  GALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQSTTENYWL 358
            GA+ATMDR IHGFFTHGLAETHVAHHL S+IPHY+AWDAT+ALK KLG HY S+ EN+W 
Sbjct: 355  GAIATMDRKIHGFFTHGLAETHVAHHLFSRIPHYNAWDATDALKVKLGKHYNSSDENFWY 414

Query: 357  SLWKVFRHCRFVEDEGDVVFYKNAKGQAA---IKLKSSNGSASDSGIEVDQ 214
            SLWK FR CRFVEDEGDVVFYKNAKG+AA   +     +  ASDSG++VD+
Sbjct: 415  SLWKCFRQCRFVEDEGDVVFYKNAKGKAARRYVPRDIISIDASDSGVDVDE 465


>XP_007403496.1 hypothetical protein MELLADRAFT_70376 [Melampsora larici-populina
            98AG31] EGG12558.1 hypothetical protein MELLADRAFT_70376
            [Melampsora larici-populina 98AG31]
          Length = 452

 Score =  583 bits (1504), Expect = 0.0
 Identities = 271/441 (61%), Positives = 336/441 (76%), Gaps = 2/441 (0%)
 Frame = -3

Query: 1527 ASPKVIHLAXXXXXXXXXXXXXSHLPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIR 1348
            ++P +IHL+               L +F PP FTIKELL AIPSHCF+RS I+SFSY+I 
Sbjct: 15   SNPPIIHLSRSSSSSSLSNQDT--LSSFTPPDFTIKELLAAIPSHCFERSAIKSFSYLIW 72

Query: 1347 DLAFTAVLVYAATFIDSNFASQGGKILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHE 1168
            DL  ++VL+YAA+FID+ F   G  I++G LG + + A W  Y Y++GLVWTG+WVLAHE
Sbjct: 73   DLFLSSVLIYAASFIDTKFGGSGS-IINGHLGRLVSLAAWSLYAYSIGLVWTGIWVLAHE 131

Query: 1167 CGHQSFSPSKTINYWTGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLN 988
            CGHQSFS SKT+N   GW LHSALLVP+HSWRITH+QHHA+T HMS+DQ FVP TR+Q  
Sbjct: 132  CGHQSFSTSKTLNNTVGWFLHSALLVPYHSWRITHSQHHAATGHMSRDQVFVPKTRTQRG 191

Query: 987  LPPLSADASEREIKGAMAPTFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQK 808
            + PL  +A++RE  GA+  + YE++DDLLEDAP++AL  L + Q VG+ AY+L NASGQ 
Sbjct: 192  VKPLPENATKRERDGALDQSLYEKMDDLLEDAPIYALINLTIQQLVGWPAYILMNASGQA 251

Query: 807  HYPPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLW 628
             YP +TNHFNP ++M+  RHR Q++ SD+GI I    L L G++T  L V+KYY+VPY W
Sbjct: 252  RYPSWTNHFNPQSIMFDKRHRSQVLLSDLGIGIMFTILFLFGLRTSFLNVVKYYVVPYFW 311

Query: 627  VHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSK 448
            V+HW+VLIT+LQHTDP LPHYREGAFNFQRGAL T+DR IHGFF HG+AETHVAHHLCSK
Sbjct: 312  VNHWLVLITYLQHTDPNLPHYREGAFNFQRGALCTIDRKIHGFFFHGIAETHVAHHLCSK 371

Query: 447  IPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQA-- 274
            IPHY+AWDATEALK+KLG HY ST EN+W SLWK +  CRFVEDEGDVVFYKNAKG+A  
Sbjct: 372  IPHYNAWDATEALKKKLGDHYMSTDENFWYSLWKSYTQCRFVEDEGDVVFYKNAKGKAMR 431

Query: 273  AIKLKSSNGSASDSGIEVDQK 211
             + +       SDSG++V +K
Sbjct: 432  RVVISDKKDQLSDSGVDVGEK 452


>OAV91880.1 hypothetical protein PTTG_02647 [Puccinia triticina 1-1 BBBD Race 1]
          Length = 458

 Score =  571 bits (1471), Expect = 0.0
 Identities = 279/456 (61%), Positives = 332/456 (72%), Gaps = 3/456 (0%)
 Frame = -3

Query: 1575 SNLDAPPNTLTRRSGNASPKVIHLAXXXXXXXXXXXXXSH-LPTFEPPPFTIKELLGAIP 1399
            S    PP+TL  R+      VIHLA                LP FE P FTIKELL AIP
Sbjct: 2    SEAQEPPSTLHHRTQPVP--VIHLAGSSASSASDDEDNQSSLPIFELPQFTIKELLSAIP 59

Query: 1398 SHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKILDGKLGAMANFAIWGAY 1219
            +HCF RS +RS  Y++ D+   +  +Y A FID  F S      +  L  +   A W  Y
Sbjct: 60   AHCFQRSALRSGVYLLWDIFLASSFIYLARFIDPFFDSLTTS--NPSLVTLGRIAAWAFY 117

Query: 1218 GYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLVPFHSWRITHAQHHASTS 1039
            GYA GLVWTGVWVLAHECGHQ+FS SKTIN   G++ HS LLVP+HSWRI+HAQHHA+T 
Sbjct: 118  GYASGLVWTGVWVLAHECGHQAFSSSKTINNTVGFIFHSLLLVPYHSWRISHAQHHAATC 177

Query: 1038 HMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERVDDLLEDAPLWALSQLFVH 859
            HMS+DQ FVP TRSQ   PPL ++AS+RE +G + P+ YE +DDLLEDAPLW L  LF+ 
Sbjct: 178  HMSRDQVFVPKTRSQRGAPPLPSNASKREREGTLPPSLYETMDDLLEDAPLWNLFNLFLQ 237

Query: 858  QSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGM 679
            Q  G+  Y+L NASGQ  YP +TNHFNP +++Y  RHR QI+ SD+GI +T+  L +LG 
Sbjct: 238  QLFGWPMYILSNASGQARYPSWTNHFNPNSIIYDARHRSQILKSDLGIGLTLAGLTILGY 297

Query: 678  KTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHGF 499
            KTD LTV KYYIVPY  V+HW+V+ITFLQH DP+LPHYREGAFNFQRGAL T+DRNIHGF
Sbjct: 298  KTDFLTVFKYYIVPYFNVNHWLVMITFLQHNDPMLPHYREGAFNFQRGALCTIDRNIHGF 357

Query: 498  FTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVE 319
            F HG+AETHVAHH+CSKIPHYHAWDATEA+K KLG HY+ + EN W+SLWKV+  CRFVE
Sbjct: 358  FFHGIAETHVAHHICSKIPHYHAWDATEAIKAKLGAHYRKSDENAWVSLWKVYNQCRFVE 417

Query: 318  DEGDVVFYKNAKGQAAIKLKS--SNGSASDSGIEVD 217
            DEGDVVF+KNAKGQAA +L S  S GS SDSG+EV+
Sbjct: 418  DEGDVVFWKNAKGQAACRLASPGSKGSVSDSGVEVN 453


>KNZ62768.1 omega-6 fatty acid desaturase (Delta-12 desaturase) [Puccinia sorghi]
          Length = 458

 Score =  559 bits (1441), Expect = 0.0
 Identities = 263/415 (63%), Positives = 316/415 (76%), Gaps = 2/415 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +P FE P FTIKELL AIP+HCF RS +RS  Y++ DL   +  +Y A FID  F S   
Sbjct: 39   VPIFELPQFTIKELLSAIPAHCFQRSALRSSVYLVWDLFLASSFIYLARFIDPFFNSLAS 98

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
              ++  L ++  FA W  Y YA GLVWTG+WV+AHECGHQ+FS SK+IN   GWVLHSAL
Sbjct: 99   --INPTLVSLGRFAAWALYAYASGLVWTGIWVIAHECGHQAFSTSKSINNSVGWVLHSAL 156

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVP+HSWRI+HAQHHA+T HM++DQ FVP TRSQ  LP L  +AS+RE  GA+ P+ YE+
Sbjct: 157  LVPYHSWRISHAQHHAATCHMTRDQVFVPKTRSQRGLPALPPNASKRERDGALPPSLYEK 216

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            +DDLLEDAPLW    L + Q +G+  Y+  NASGQ  YP +TNHFNP +++Y  RHR QI
Sbjct: 217  MDDLLEDAPLWNFINLCLQQLLGWPLYMFTNASGQARYPRWTNHFNPNSIIYDARHRSQI 276

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
            + SD+GI + +  L   G KTD +TV KYYIVPYL V+HW+V+ITFLQH+DPL+PHYR+G
Sbjct: 277  LKSDLGIGLMLAGLTFFGYKTDFVTVFKYYIVPYLIVNHWLVMITFLQHSDPLIPHYRDG 336

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            AFNFQRGAL TMDRNIH FF HG+AETHVAHHLCSKIPHYHAWDAT ALK KLG HY  T
Sbjct: 337  AFNFQRGALCTMDRNIHSFFFHGIAETHVAHHLCSKIPHYHAWDATAALKAKLGSHYLQT 396

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKL--KSSNGSASDSGIEVD 217
             EN W+SLWKVF  CRFVED+GDVVF+KNAKGQA  +L   SS GS SDSG+E++
Sbjct: 397  DENAWVSLWKVFNECRFVEDDGDVVFWKNAKGQAHRRLGSPSSKGSVSDSGVEIN 451


>XP_003307171.1 omega-6 fatty acid desaturase (delta-12 desaturase) [Puccinia
            graminis f. sp. tritici CRL 75-36-700-3] EFP74165.1
            omega-6 fatty acid desaturase (delta-12 desaturase)
            [Puccinia graminis f. sp. tritici CRL 75-36-700-3]
          Length = 453

 Score =  558 bits (1438), Expect = 0.0
 Identities = 263/415 (63%), Positives = 316/415 (76%), Gaps = 2/415 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +P FE P FTIKELL AIP+HCF RS +RS  Y+I D+   +  +Y A FID  F S   
Sbjct: 35   IPIFELPQFTIKELLSAIPAHCFQRSALRSSVYLIWDIFLASSFIYLARFIDPYFNSLSN 94

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
               +  L  +A FA W  YGYA GLVWTG+WV+AHECGHQ+FS SK+IN   G+VLHS L
Sbjct: 95   TT-NPTLITLARFAAWAFYGYASGLVWTGIWVIAHECGHQAFSSSKSINNTVGYVLHSFL 153

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVP+HSWRI+HAQHHA+T HM +DQ FVP TRS+  +PPL  +AS+RE  GA+ P+ YE+
Sbjct: 154  LVPYHSWRISHAQHHAATCHMKRDQVFVPKTRSERGVPPLPENASKRERDGALPPSLYEK 213

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            +DDLLEDAPLW    L + Q +G+  Y+L NASGQK YP +TNHFNP +++Y  RHR QI
Sbjct: 214  MDDLLEDAPLWNFLNLLLQQLLGWPMYILTNASGQKRYPSWTNHFNPNSIIYDARHRSQI 273

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
            + SD+GI I +  L  LG KTD +TV KYYIVPY  V+HW+V+ITFLQH DP+LPHYR+G
Sbjct: 274  LKSDLGIGIMLAGLTFLGYKTDFMTVFKYYIVPYFNVNHWLVMITFLQHNDPMLPHYRDG 333

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            AFNFQRGAL T+DRNIH FF HG+AETHVAHHLCSKIPHYHAWDAT ALK KLG HY  T
Sbjct: 334  AFNFQRGALCTIDRNIHSFFFHGIAETHVAHHLCSKIPHYHAWDATAALKAKLGPHYHQT 393

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKLKS--SNGSASDSGIEVD 217
             E+ W+SLWKV+  CRFVEDEGDVVF+KNAKGQA  +L S  S GS SDSG+E++
Sbjct: 394  DEDAWISLWKVYSQCRFVEDEGDVVFWKNAKGQAHRRLGSPGSKGSVSDSGVEIN 448


>KNF00291.1 hypothetical protein PSTG_06464 [Puccinia striiformis f. sp. tritici
            PST-78]
          Length = 456

 Score =  550 bits (1416), Expect = 0.0
 Identities = 258/415 (62%), Positives = 317/415 (76%), Gaps = 2/415 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            LP FE P F++KELL AIP+HCF RS +RS  Y++ D+  +   +Y ATFID  F +   
Sbjct: 37   LPIFELPEFSVKELLSAIPAHCFHRSALRSSLYLLWDIFLSVSFIYLATFIDPYFNTLTS 96

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
              ++  L  +A FA W  YGYA+GLVWTG+WV+AHECGHQ+FS SKTIN   G VLHS L
Sbjct: 97   --INPALVGLARFAAWAFYGYAIGLVWTGIWVIAHECGHQAFSSSKTINNTVGLVLHSFL 154

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVP+HSWRI+HAQHHA+T H+++DQ FVP TRS   LP L  +AS+RE  GA+  + YE+
Sbjct: 155  LVPYHSWRISHAQHHAATCHLTRDQVFVPKTRSARGLPALPENASKRERDGALPSSLYEK 214

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            +DDLLEDAP W    L + Q VG+  Y+  NASGQ  YP +TNHFNP A+++  RHR Q+
Sbjct: 215  MDDLLEDAPFWNFLNLALQQLVGWLLYMTMNASGQPRYPSWTNHFNPNAIIFDARHRSQV 274

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
              S IGI + + +L ++G+KTD +TV KYYIVPY  V+HW+V+ITFLQHTDPL+PHYR+G
Sbjct: 275  TNSVIGIGLMLTALTVMGIKTDFMTVFKYYIVPYFHVNHWLVMITFLQHTDPLIPHYRDG 334

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            AFNFQRGAL T+DRNIH FF HG+AETHVAHHLCSKIPHY+AWDAT A+KEKLG HY  T
Sbjct: 335  AFNFQRGALCTIDRNIHSFFFHGIAETHVAHHLCSKIPHYNAWDATAAIKEKLGKHYHQT 394

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKLKS--SNGSASDSGIEVD 217
             EN WLSLWKV+  CRFVEDEGDVVF+KNAKGQAA +L S  S GS SDSG+E++
Sbjct: 395  DENAWLSLWKVYNQCRFVEDEGDVVFWKNAKGQAARRLGSPGSKGSVSDSGVEIN 449


>KNF00278.1 hypothetical protein PSTG_06449 [Puccinia striiformis f. sp. tritici
            PST-78]
          Length = 457

 Score =  528 bits (1361), Expect = 0.0
 Identities = 250/415 (60%), Positives = 310/415 (74%), Gaps = 2/415 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            LP FE P FT+KELL AIP+HCF RS+IRS  ++I DL     L+Y ATFID  F +   
Sbjct: 40   LPIFELPDFTVKELLAAIPAHCFQRSVIRSSLHLILDLTMAGSLIYMATFIDPYFNTL-- 97

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
               +  + ++A FA W  YGYA GLVW G+WV+AHECGHQ+FS  KT+N   G +LHS L
Sbjct: 98   TTFNPTVVSLARFASWAFYGYASGLVWVGIWVIAHECGHQAFSSHKTLNNTIGLILHSIL 157

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVP+HSWRI+HA+HHAST H+++DQ FVP TRS++   P S  AS+ E +G + P+ YE+
Sbjct: 158  LVPYHSWRISHARHHASTCHLTRDQVFVPKTRSEVGALPASEHASQSEREGHLPPSLYEK 217

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            +D+L EDAPLW   +L + Q  G++ Y+L NASGQ  YP +TNHFNP A++Y +RHR Q+
Sbjct: 218  MDNLFEDAPLWNFFELTLQQLFGWSMYILVNASGQSSYPSWTNHFNPNAIIYDERHRSQV 277

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
            + SDIG+ + +  L  LG+KTD +TV KYYIVPYL V+HWIVLITF+QHTDPLLPHYR+G
Sbjct: 278  LKSDIGVGLMLTGLTALGLKTDFMTVFKYYIVPYLNVNHWIVLITFIQHTDPLLPHYRDG 337

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            AFNFQRGALAT+DR IHGFF HG+AETH  HHLCSKIPHY+AW+AT A+KEKLG HY   
Sbjct: 338  AFNFQRGALATIDRKIHGFFFHGIAETHTVHHLCSKIPHYNAWEATAAIKEKLGKHYHYD 397

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKLKS--SNGSASDSGIEVD 217
              + W+SLWKV   CRFVEDEGDVVF+KNAKGQA  +L    S  S S SG +VD
Sbjct: 398  DRSAWVSLWKVHTQCRFVEDEGDVVFWKNAKGQAFCRLADPVSKESVSVSGDKVD 452


>XP_014566653.1 hypothetical protein L969DRAFT_95919 [Mixia osmundae IAM 14324]
            GAA95111.1 hypothetical protein E5Q_01766 [Mixia osmundae
            IAM 14324] KEI38080.1 hypothetical protein
            L969DRAFT_95919 [Mixia osmundae IAM 14324]
          Length = 461

 Score =  489 bits (1260), Expect = e-166
 Identities = 235/414 (56%), Positives = 300/414 (72%), Gaps = 5/414 (1%)
 Frame = -3

Query: 1446 FEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGKIL 1267
            F PP FTIKELLG IP HCF+RS +RS SY+  DLA  A+ V+AA+ ID++F+ QG  +L
Sbjct: 47   FIPPTFTIKELLGCIPKHCFERSALRSGSYIAADLALIAMWVFAASHIDAHFSKQGS-VL 105

Query: 1266 DGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALLVP 1087
            DG LG    +A+W  Y +  GL   G+WV+AHECGHQ+FSPSKTIN   GWVLHSALLVP
Sbjct: 106  DGVLGQFVGYALWAGYTFGAGLNAVGLWVIAHECGHQAFSPSKTINNAVGWVLHSALLVP 165

Query: 1086 FHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMA-----PTFY 922
            +HSWRI+HA+HHA+T HM++DQ FVP TRSQ  + P    ASE E+K          + +
Sbjct: 166  YHSWRISHAKHHAATGHMTRDQVFVPKTRSQRKIAP----ASEGELKAQSEFFEGNESAF 221

Query: 921  ERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRM 742
            E++DDLLEDAP+W L  L V Q  G+ AYL+ NASGQK    +TNHF+P + ++  RH  
Sbjct: 222  EKLDDLLEDAPIWTLLNLIVQQLFGWPAYLIVNASGQK-LGRWTNHFDPNSPIFTARHTK 280

Query: 741  QIVYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYR 562
            QI+ SDIGI +T+ +L           V++YY++PYLWV+HW+VLIT+LQHTDP LPHYR
Sbjct: 281  QIIASDIGIGLTVAALTTWAHYASWTDVVRYYLIPYLWVNHWLVLITYLQHTDPTLPHYR 340

Query: 561  EGAFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQ 382
            EGAFNFQRGAL TMDRN+ G   HG+AETH+ HHLCS IPHY+AW+ATEALK+KLG +Y 
Sbjct: 341  EGAFNFQRGALCTMDRNVFGLAFHGIAETHICHHLCSAIPHYNAWEATEALKQKLGPYYN 400

Query: 381  STTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKLKSSNGSASDSGIEV 220
             + E +  SLW  +  CRFVEDEGD+VFYKN  G++  K+  S+  +SDSG ++
Sbjct: 401  KSDEFFLTSLWNSYSQCRFVEDEGDIVFYKNKNGKSIRKVARSD--SSDSGTDL 452


>KNE92012.1 hypothetical protein PSTG_14600 [Puccinia striiformis f. sp. tritici
            PST-78]
          Length = 465

 Score =  467 bits (1202), Expect = e-157
 Identities = 221/417 (52%), Positives = 289/417 (69%), Gaps = 2/417 (0%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +P FE P FT+ ELL  IP HCF+ S I+S  Y+IRDL   + LV+ A FID  F +   
Sbjct: 49   IPVFELPHFTMNELLSVIPDHCFETSAIKSSFYLIRDLLMVSTLVFLANFIDPYFDTIRP 108

Query: 1275 KILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSAL 1096
                  L ++A F  W  YGYA GLV  G+WV+AHECGH++FS  KTI+   G++LHSAL
Sbjct: 109  DSSHPSLISLARFTAWALYGYACGLVEVGLWVVAHECGHRAFSGYKTIDDSVGFILHSAL 168

Query: 1095 LVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYER 916
            LVP+HS+RITHA+HHA+T H+ +DQ FVP TR ++ L PL+ DAS  E +G M P+FYE+
Sbjct: 169  LVPYHSFRITHARHHAATGHLVRDQTFVPKTRYEVGLAPLNPDASRDEREGNMHPSFYEK 228

Query: 915  VDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQI 736
            +DDLLED+P+W L  L + Q +G+  YL+ N SGQ  YP +T+HFNP A M+  +HR Q+
Sbjct: 229  MDDLLEDSPMWNLVTLMIQQIIGWPMYLVANTSGQSRYPAWTSHFNPSAAMFDPKHRSQV 288

Query: 735  VYSDIGIAITIGSLVLLGMKTDLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
            + S +G+   + +L     +T  LTV KYYIVPYL  +HWIV++T++QH DP+LP+YR  
Sbjct: 289  LASGLGVGSMLTALGYTAHQTSFLTVFKYYIVPYLLTNHWIVMLTYVQHNDPILPNYRGS 348

Query: 555  AFNFQRGALATMDRNIHGFFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHHYQST 376
            A  F RG L  +DR  +GFF HG AE H++HH+CS+IPHYHA +AT ALK KLGHHY  +
Sbjct: 349  AHTFVRGTLCAVDRKFYGFFFHGFAENHISHHVCSRIPHYHAAEATAALKVKLGHHYNES 408

Query: 375  TENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKL--KSSNGSASDSGIEVDQK 211
             EN + SLW+ +   RFVEDEGDVVF+KNAKGQAA +L    S  S S SG+E   K
Sbjct: 409  DENCYKSLWRCYTQARFVEDEGDVVFFKNAKGQAARRLFNPDSKISGSHSGVETSDK 465


>KDE03817.1 omega-6 fatty acid desaturase (delta-12 desaturase) [Microbotryum
            lychnidis-dioicae p1A1 Lamole]
          Length = 447

 Score =  463 bits (1191), Expect = e-155
 Identities = 224/417 (53%), Positives = 297/417 (71%), Gaps = 5/417 (1%)
 Frame = -3

Query: 1452 PTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGGK 1273
            P F PP FT+K++LGAIP+HCF+RS +RS +YV+ D +  AVL YAAT ID  F S G +
Sbjct: 39   PPFTPPQFTVKDVLGAIPAHCFERSALRSSTYVVADYSMLAVLFYAATHIDILFGSNGTQ 98

Query: 1272 ILDGKLGAMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYWTGWVLHSALL 1093
             L G  G +A +A W  Y    G V TG+W++AHECGHQSFSPSKTIN   G VLHS +L
Sbjct: 99   -LQGAAGVVAKWAAWSVYWILAGWVMTGIWIIAHECGHQSFSPSKTINNTVGLVLHSFVL 157

Query: 1092 VPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSADASEREIKGAMAPTFYERV 913
            VP+HSWRI+HA+HHA+T HM++D+ FVP T+S+       A  + R++           +
Sbjct: 158  VPYHSWRISHAKHHAATGHMNRDEVFVPATKSE---KVTKAAGTGRKV---FVENLGAEL 211

Query: 912  DDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYPPYTNHFNPWAVMYGDRHRMQIV 733
            D+LLEDAP + L  L V Q  G+ AYL  NASGQ  YP  TNHFNP ++++  RHR Q++
Sbjct: 212  DELLEDAPAYRLFHLLVQQLFGWPAYLFSNASGQNWYPKGTNHFNPNSLIFDARHRQQVL 271

Query: 732  YSDIGIAITIGSLVLLGMKT-DLLTVIKYYIVPYLWVHHWIVLITFLQHTDPLLPHYREG 556
             SD G+   + +L   G+K      V+KYY +PYL+V+HW+V+IT+LQHTDP+LPHYR  
Sbjct: 272  ISDAGLIAMLSALTWFGLKMGGFSAVVKYYGIPYLFVNHWLVMITYLQHTDPMLPHYRAN 331

Query: 555  AFNFQRGALATMDRNIHG----FFTHGLAETHVAHHLCSKIPHYHAWDATEALKEKLGHH 388
             +NFQRGA+ T+DRN+ G    +  HG+ ETHVAHH+CSKIPHYHAW+ATEALKE LG H
Sbjct: 332  EWNFQRGAICTIDRNMLGPVGPYLMHGICETHVAHHVCSKIPHYHAWEATEALKEFLGEH 391

Query: 387  YQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQAAIKLKSSNGSASDSGIEVD 217
            YQST EN ++SLWK FR CR+V+DEGDVVF+++A G+ + K+  ++ + SDSG+EV+
Sbjct: 392  YQSTNENMFVSLWKNFRTCRYVDDEGDVVFFRDALGRTSRKVVPAD-APSDSGVEVN 447


>CBQ70450.1 related to Delta-12 fatty acid desaturase [Sporisorium reilianum
            SRZ2]
          Length = 487

 Score =  461 bits (1186), Expect = e-154
 Identities = 231/442 (52%), Positives = 299/442 (67%), Gaps = 28/442 (6%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +PTF+ P FT+K+LL AIP+HCF+RS  +SF+YV  D A  A L +AA++ID   AS   
Sbjct: 47   IPTFDVPSFTVKDLLSAIPAHCFERSAFKSFTYVFADFAMIAALGFAASYIDPTVASTFS 106

Query: 1275 KILDGKLG---------AMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYW 1123
             I    L          A+  FA W AY    G+V+TGVWV+AHECGHQSFS SKT+N  
Sbjct: 107  GIESNALSPYVSLGVQSAVTRFAAWSAYWILQGMVFTGVWVIAHECGHQSFSTSKTLNNA 166

Query: 1122 TGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPL----------- 976
             GWVLHS+LLVP+HSWRI+HA+HHA+T H+++D+ FVP TRSQ    PL           
Sbjct: 167  VGWVLHSSLLVPYHSWRISHARHHAATGHLTRDEVFVPRTRSQRGRLPLQPAPKSGDDSN 226

Query: 975  SADASEREIKGA--MAPTFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHY 802
              DA E+E+ GA  +  TF E + ++LEDAPL+ L+ +FV Q +G+  YLLRNASGQ HY
Sbjct: 227  DEDAVEKEV-GAPHVDETFGEWLSEILEDAPLYNLTYIFVQQLLGWPLYLLRNASGQLHY 285

Query: 801  PPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGM--KTDLLTVIKYYIVPYLW 628
            P YTNHFNP A+++  RHRMQIV SDIGIA T+ +L   GM  K     V +YY++PYL+
Sbjct: 286  PKYTNHFNPDAIIFDKRHRMQIVVSDIGIAATLSALTAWGMLSKGGFSDVFRYYVIPYLF 345

Query: 627  VHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHG----FFTHGLAETHVAHH 460
             +HW+V+IT+LQHTDP LPHY+  A+ F RGAL T+DRN  G    +  HG+AETHV HH
Sbjct: 346  CNHWLVMITYLQHTDPALPHYKAEAWTFPRGALCTIDRNWLGPVGPYLFHGIAETHVLHH 405

Query: 459  LCSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKG 280
            + SKIPHYHAW+ATEALK +LGHHY  +TEN ++SLWK    C+FV++   V FY+   G
Sbjct: 406  ISSKIPHYHAWEATEALKVRLGHHYVKSTENVFVSLWKSINTCKFVDENDQVAFYRTVDG 465

Query: 279  QAAIKLKSSNGSASDSGIEVDQ 214
                 +   +  ASDSGI + +
Sbjct: 466  VPHRVVSPDSTYASDSGIAMSE 487


>XP_011387393.1 hypothetical protein UMAG_11270 [Ustilago maydis 521] KIS71136.1
            hypothetical protein UMAG_11270 [Ustilago maydis 521]
          Length = 490

 Score =  457 bits (1176), Expect = e-152
 Identities = 227/442 (51%), Positives = 295/442 (66%), Gaps = 28/442 (6%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +PTF+ P FT+K+LL AIP+HCF+RS  +SF+YV  D A  A L YAA+FID   AS   
Sbjct: 50   IPTFDVPQFTVKDLLSAIPAHCFERSAFKSFTYVFADFAMIAALGYAASFIDPTVASTFS 109

Query: 1275 KILDGKLG---------AMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYW 1123
             +    L          A   FA W AY    G+V+TGVWV+AHECGHQSFS SKT+N  
Sbjct: 110  GLKSNALSPYVSLGVQQAATRFAAWSAYWILQGMVFTGVWVIAHECGHQSFSTSKTLNNA 169

Query: 1122 TGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPL----------- 976
             GWVLHSALLVP+HSWRI+HA+HHA+T H+++D+ FVP TR Q    PL           
Sbjct: 170  VGWVLHSALLVPYHSWRISHARHHAATGHLTRDEVFVPRTRQQRGRLPLQPAPKSADDSN 229

Query: 975  SADASEREIKGAMA--PTFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHY 802
              DA E+E+ GA+    TF E + ++LEDAP + L  +F+ Q +G+  YLLRNASGQ HY
Sbjct: 230  DEDAVEKEV-GAIKVDETFGEWLAEVLEDAPAYNLLYIFIQQLLGWPLYLLRNASGQLHY 288

Query: 801  PPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGM--KTDLLTVIKYYIVPYLW 628
            P +TNHFNP A+++  RHRMQI+ SDIGIA T+ +L   G+  K     V +YY++PYLW
Sbjct: 289  PKFTNHFNPDAIIFDKRHRMQIIVSDIGIAATLSALTAWGLLSKGGFSDVFRYYVIPYLW 348

Query: 627  VHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHG----FFTHGLAETHVAHH 460
             +HW+V+IT+LQHTDP LPHY+  A+ F RGAL T+DRN  G    +  HG+AETHV HH
Sbjct: 349  CNHWLVMITYLQHTDPALPHYKAEAWTFPRGALCTIDRNWLGPVGPYLFHGIAETHVLHH 408

Query: 459  LCSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKG 280
            + SKIPHY+AW+ATEALK +LGHHY  +TEN ++SLWK    C+FV++   V FY+   G
Sbjct: 409  VSSKIPHYNAWEATEALKARLGHHYVKSTENVFVSLWKSINTCKFVDENDQVAFYRTVDG 468

Query: 279  QAAIKLKSSNGSASDSGIEVDQ 214
                 +   +  ASDSGI + +
Sbjct: 469  VPHRVISPDSAYASDSGIAMSE 490


>XP_012190813.1 likely delta-12 fatty acid desaturase [Pseudozyma hubeiensis SY62]
            GAC97226.1 likely delta-12 fatty acid desaturase
            [Pseudozyma hubeiensis SY62]
          Length = 488

 Score =  455 bits (1170), Expect = e-152
 Identities = 225/442 (50%), Positives = 294/442 (66%), Gaps = 28/442 (6%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +PTF+ P FT+K+LL AIP+HCF+RS  +SF+YV  D A  A L YAA+FID   AS   
Sbjct: 47   IPTFDVPQFTVKDLLSAIPAHCFERSAFKSFTYVFADFAMIAALGYAASFIDPTVASTFS 106

Query: 1275 KILDGKLG---------AMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYW 1123
             +    +          A   FA W AY    G+V+TGVWV+AHECGHQSFS SKT+N  
Sbjct: 107  GLKSNAVSPYVSLGVQQAAVRFAAWSAYWILQGMVFTGVWVIAHECGHQSFSTSKTLNNA 166

Query: 1122 TGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPL----------- 976
             GWVLHSALLVP+HSWRI+HA+HHA+T H+++D+ FVP TR Q    PL           
Sbjct: 167  VGWVLHSALLVPYHSWRISHARHHAATGHLTRDEVFVPRTRQQRGRLPLQPAPQSADDSN 226

Query: 975  SADASEREIKGAMA--PTFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHY 802
              DA E+E+  ++    TF E + ++LEDAP + L  +F+ Q +G+  YLLRNASGQ HY
Sbjct: 227  DEDAVEKEVGASVQADETFGEWLAEILEDAPAYNLLYIFIQQLLGWPLYLLRNASGQLHY 286

Query: 801  PPYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGM--KTDLLTVIKYYIVPYLW 628
            P +TNHFNP A+++  RHR QIV SDIGIA T+ +L   GM  K     V +YY++PYLW
Sbjct: 287  PKFTNHFNPDAIIFDKRHRTQIVVSDIGIAATLSALTAWGMLSKGGFSDVFRYYVIPYLW 346

Query: 627  VHHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHG----FFTHGLAETHVAHH 460
             ++W+V+IT+LQHTDP LPHY+  A+ F RGAL T+DRN  G    +  HG+AETHV HH
Sbjct: 347  CNNWLVMITYLQHTDPALPHYKAEAWTFPRGALCTIDRNWLGPVGPYLFHGIAETHVLHH 406

Query: 459  LCSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKG 280
            + SKIPHY+AW+ATEALKE+LGHHY  +TEN ++SLWK    C+FV++   V FY+   G
Sbjct: 407  VSSKIPHYNAWEATEALKERLGHHYVKSTENIFVSLWKSINTCKFVDENDQVAFYRTVDG 466

Query: 279  QAAIKLKSSNGSASDSGIEVDQ 214
                 +   +  ASDSGI + +
Sbjct: 467  VPHRVISPDSTYASDSGIAMSE 488


>CCF54793.1 related to Delta-12 fatty acid desaturase [Ustilago hordei]
          Length = 489

 Score =  452 bits (1164), Expect = e-151
 Identities = 224/441 (50%), Positives = 295/441 (66%), Gaps = 27/441 (6%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +PTF+ P +T+K+LL AIP+HCF+RS  +SF+YV  D A  A L +AA++ID   AS   
Sbjct: 49   IPTFDVPQYTVKDLLSAIPAHCFERSAFKSFTYVFADFAMIAALGFAASYIDPTVASTFS 108

Query: 1275 KILDGKLG---------AMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYW 1123
             +    L          A+A FA W  Y    G+V+TGVWV+AHECGHQSFS SKTIN  
Sbjct: 109  GLKSNALSPYISLGVQQALARFAAWSTYWILQGMVFTGVWVIAHECGHQSFSTSKTINNA 168

Query: 1122 TGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPLSA------DAS 961
             GWVLHSALLVP+HSWRI+HA+HHA+T H+++D+ FVP TR Q    PL        D++
Sbjct: 169  VGWVLHSALLVPYHSWRISHARHHAATGHLTRDEVFVPRTRQQRGRLPLQPAPKSGDDSN 228

Query: 960  EREI--KGAMAP----TFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYP 799
            + ++  K   AP    T+ E + ++LEDAPL+ L  +FV Q +G+  YLLRNASGQ HYP
Sbjct: 229  DEDVVEKEVGAPNVDETWGEWLSEVLEDAPLYNLFYIFVQQLLGWPLYLLRNASGQLHYP 288

Query: 798  PYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGM--KTDLLTVIKYYIVPYLWV 625
             YTNHFNP A+++  RHR QI+ SDIGIA T+ +L   GM  K     V +YY++PY W 
Sbjct: 289  KYTNHFNPDAIIFDKRHRSQIIVSDIGIAATLSALTAWGMLSKGGFSDVFRYYVIPYFWC 348

Query: 624  HHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHG----FFTHGLAETHVAHHL 457
            +HW+V+IT+LQHTDP LPHY+  A+ F RGAL T+DRN  G    +  HG+AETHV HH+
Sbjct: 349  NHWLVMITYLQHTDPALPHYKAEAWTFPRGALCTIDRNWLGPVGPYLFHGIAETHVLHHV 408

Query: 456  CSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQ 277
             SKIPHY+AW+ATEALK +LGHHY  +TEN ++SLWK    C+FV++   V FY+   G 
Sbjct: 409  SSKIPHYNAWEATEALKARLGHHYVKSTENVFVSLWKSINTCKFVDESDQVAFYRTVDGV 468

Query: 276  AAIKLKSSNGSASDSGIEVDQ 214
                +   +  ASDSGI + +
Sbjct: 469  PHRVVSPDSTYASDSGIALSE 489


>CDW97138.1 hypothetical protein [Sporisorium scitamineum] CDU24606.1 related to
            Delta-12 fatty acid desaturase [Sporisorium scitamineum]
          Length = 489

 Score =  452 bits (1163), Expect = e-150
 Identities = 224/441 (50%), Positives = 296/441 (67%), Gaps = 27/441 (6%)
 Frame = -3

Query: 1455 LPTFEPPPFTIKELLGAIPSHCFDRSLIRSFSYVIRDLAFTAVLVYAATFIDSNFASQGG 1276
            +PTF+ P FT+K+LL AIP+HCF+RS  +SF+YV  D A  A L +AA++ID   AS   
Sbjct: 49   IPTFDVPSFTVKDLLSAIPAHCFERSAFKSFTYVFADFAMIAALGFAASYIDPTVASTFS 108

Query: 1275 KILDGKLG---------AMANFAIWGAYGYALGLVWTGVWVLAHECGHQSFSPSKTINYW 1123
             +    L          A+A FA W AY    G+V+TGVWV+AHECGHQSFS SKT+N  
Sbjct: 109  GLKSNALSPYVSLGVQSALARFAAWSAYWILQGMVFTGVWVIAHECGHQSFSTSKTLNNA 168

Query: 1122 TGWVLHSALLVPFHSWRITHAQHHASTSHMSKDQAFVPYTRSQLNLPPL----------- 976
             GWVLHSALLVP+HSWRI+HA+HHA+T H+++D+ FVP TRSQ    PL           
Sbjct: 169  VGWVLHSALLVPYHSWRISHARHHAATGHLTRDEVFVPRTRSQRGRLPLQPAPKSGDDSN 228

Query: 975  SADASEREIKGA-MAPTFYERVDDLLEDAPLWALSQLFVHQSVGFAAYLLRNASGQKHYP 799
              DA E+EI    +  TF E + ++LEDAPL+ L+ +FV Q  G+  YL++NASGQ HYP
Sbjct: 229  DEDAVEKEIGAVHVEETFGEWLSEILEDAPLYNLTYIFVQQLFGWPMYLIKNASGQLHYP 288

Query: 798  PYTNHFNPWAVMYGDRHRMQIVYSDIGIAITIGSLVLLGMKTD--LLTVIKYYIVPYLWV 625
              TNHFNP A+++  RHRMQI+ SDIGIA T+ +L   GM +      V +YY++PYL+ 
Sbjct: 289  KGTNHFNPDAIIFDKRHRMQIIVSDIGIAATLSALTAWGMLSQGGFSDVFRYYVIPYLFC 348

Query: 624  HHWIVLITFLQHTDPLLPHYREGAFNFQRGALATMDRNIHG----FFTHGLAETHVAHHL 457
            +HW+V+IT+LQHTDP LPHY+  A+ F RGAL T+DRN  G    +  HG+AETHV HH+
Sbjct: 349  NHWLVMITYLQHTDPALPHYKAEAWTFPRGALCTIDRNWLGPVGPYLFHGIAETHVLHHV 408

Query: 456  CSKIPHYHAWDATEALKEKLGHHYQSTTENYWLSLWKVFRHCRFVEDEGDVVFYKNAKGQ 277
             SKIPHY+AW+ATEALK +LG HY  +TEN ++SLWK    C+FV++   V FY+   G 
Sbjct: 409  SSKIPHYNAWEATEALKARLGPHYVKSTENVFVSLWKSINTCKFVDENDQVAFYRTVDGV 468

Query: 276  AAIKLKSSNGSASDSGIEVDQ 214
               ++   +  ASDSGI + +
Sbjct: 469  PHRRISPDSTYASDSGIAMSE 489