BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Perilla23_contig00025080
         (806 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011074974.1| PREDICTED: non-lysosomal glucosylceramidase-...   378   e-102
ref|XP_009760235.1| PREDICTED: non-lysosomal glucosylceramidase-...   350   9e-94
ref|XP_009606628.1| PREDICTED: non-lysosomal glucosylceramidase-...   348   3e-93
ref|XP_009760240.1| PREDICTED: non-lysosomal glucosylceramidase-...   345   2e-92
ref|XP_009760220.1| PREDICTED: non-lysosomal glucosylceramidase-...   345   2e-92
ref|XP_009606630.1| PREDICTED: non-lysosomal glucosylceramidase-...   343   8e-92
ref|XP_009606627.1| PREDICTED: non-lysosomal glucosylceramidase-...   343   8e-92
ref|XP_006353596.1| PREDICTED: non-lysosomal glucosylceramidase-...   337   5e-90
ref|XP_010313056.1| PREDICTED: LOW QUALITY PROTEIN: non-lysosoma...   336   1e-89
ref|XP_009760264.1| PREDICTED: non-lysosomal glucosylceramidase-...   334   4e-89
ref|XP_009760255.1| PREDICTED: non-lysosomal glucosylceramidase-...   334   4e-89
ref|XP_010645881.1| PREDICTED: non-lysosomal glucosylceramidase ...   313   1e-82
ref|XP_011036252.1| PREDICTED: non-lysosomal glucosylceramidase ...   310   8e-82
ref|XP_011036249.1| PREDICTED: non-lysosomal glucosylceramidase ...   310   8e-82
ref|XP_002325943.2| hypothetical protein POPTR_0019s10160g [Popu...   310   8e-82
ref|XP_011036251.1| PREDICTED: non-lysosomal glucosylceramidase ...   310   1e-81
ref|XP_007019883.1| Beta-glucosidase, GBA2 type family protein i...   302   2e-79
ref|XP_007019882.1| Beta-glucosidase, GBA2 type family protein i...   302   2e-79
ref|XP_007019881.1| Non-lysosomal glucosylceramidase isoform 1 [...   302   2e-79
ref|XP_010246140.1| PREDICTED: non-lysosomal glucosylceramidase ...   298   4e-78

>ref|XP_011074974.1| PREDICTED: non-lysosomal glucosylceramidase-like [Sesamum indicum]
           gi|747057354|ref|XP_011074975.1| PREDICTED:
           non-lysosomal glucosylceramidase-like [Sesamum indicum]
          Length = 958

 Score =  378 bits (971), Expect = e-102
 Identities = 176/237 (74%), Positives = 202/237 (85%)
 Frame = -1

Query: 713 KVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLFRHIKEEADKGTPSVVDPYHKR 534
           K P LTW+RKLN +   P E+SVR+ E+LH++PLGWRL+RHIKEEADKGTPS+VDPYHKR
Sbjct: 33  KPPPLTWQRKLNCNGTAPTEFSVRLLEILHLIPLGWRLWRHIKEEADKGTPSIVDPYHKR 92

Query: 533 LLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDTPILANQFSAFISRPDGRQSST 354
           L++CYHG+PL        GRSFKGEFQRFQLFPR+CED PILANQFS F+SRP+G  SST
Sbjct: 93  LVTCYHGIPLGGIGAGSIGRSFKGEFQRFQLFPRICEDAPILANQFSVFVSRPNGGTSST 152

Query: 353 VLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHALFPRAWTVYDGLPDPEITITCR 174
           VLCST+P  + + K    G GI+SWDWNLD EKCTYHAL+PRAWTVYDG+P+PEI+I CR
Sbjct: 153 VLCSTSPNIIEENK----GAGIDSWDWNLDGEKCTYHALYPRAWTVYDGVPEPEISIVCR 208

Query: 173 QISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFTWANSVGGDSGSTGGHLNE 3
           QISPF+PHNYKESSFPVAVFTFT+ NSGKT ADATLLFTWANSVGG SGSTG HLN+
Sbjct: 209 QISPFIPHNYKESSFPVAVFTFTLFNSGKTCADATLLFTWANSVGGVSGSTGYHLNK 265


>ref|XP_009760235.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X2
           [Nicotiana sylvestris]
          Length = 950

 Score =  350 bits (897), Expect = 9e-94
 Identities = 171/258 (66%), Positives = 200/258 (77%), Gaps = 6/258 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P LTW+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 6   LENGVDEEERRDSLLEKLDPGKPPLLTWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 65

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 66  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 125

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 126 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDREKCTYHAL 181

Query: 236 FPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFT 57
           +PRAWTVYDG+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLFT
Sbjct: 182 YPRAWTVYDGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLFT 241

Query: 56  WANSVGGDSGSTGGHLNE 3
           WANSVGG S  +GGH+N+
Sbjct: 242 WANSVGGISEFSGGHVNK 259


>ref|XP_009606628.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X2
           [Nicotiana tomentosiformis]
          Length = 971

 Score =  348 bits (892), Expect = 3e-93
 Identities = 170/258 (65%), Positives = 199/258 (77%), Gaps = 6/258 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P L W+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 27  LENGVDEEEGRDSLLQKVDPGKPPLLAWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 86

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 87  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 146

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 147 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDGEKCTYHAL 202

Query: 236 FPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFT 57
           +PRAWTVYDG+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLFT
Sbjct: 203 YPRAWTVYDGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLFT 262

Query: 56  WANSVGGDSGSTGGHLNE 3
           WANSVGG S  +GGH+N+
Sbjct: 263 WANSVGGISEFSGGHVNK 280


>ref|XP_009760240.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana sylvestris] gi|698440510|ref|XP_009760242.1|
           PREDICTED: non-lysosomal glucosylceramidase-like isoform
           X3 [Nicotiana sylvestris]
           gi|698440517|ref|XP_009760247.1| PREDICTED:
           non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana sylvestris]
          Length = 947

 Score =  345 bits (885), Expect = 2e-92
 Identities = 171/259 (66%), Positives = 200/259 (77%), Gaps = 7/259 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P LTW+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 2   LENGVDEEERRDSLLEKLDPGKPPLLTWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 61

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 62  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 121

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 122 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDREKCTYHAL 177

Query: 236 FPRAWTVYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLF 60
           +PRAWTVYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLF
Sbjct: 178 YPRAWTVYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLF 237

Query: 59  TWANSVGGDSGSTGGHLNE 3
           TWANSVGG S  +GGH+N+
Sbjct: 238 TWANSVGGISEFSGGHVNK 256


>ref|XP_009760220.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X1
           [Nicotiana sylvestris] gi|698440491|ref|XP_009760228.1|
           PREDICTED: non-lysosomal glucosylceramidase-like isoform
           X1 [Nicotiana sylvestris]
          Length = 951

 Score =  345 bits (885), Expect = 2e-92
 Identities = 171/259 (66%), Positives = 200/259 (77%), Gaps = 7/259 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P LTW+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 6   LENGVDEEERRDSLLEKLDPGKPPLLTWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 65

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 66  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 125

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 126 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDREKCTYHAL 181

Query: 236 FPRAWTVYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLF 60
           +PRAWTVYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLF
Sbjct: 182 YPRAWTVYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLF 241

Query: 59  TWANSVGGDSGSTGGHLNE 3
           TWANSVGG S  +GGH+N+
Sbjct: 242 TWANSVGGISEFSGGHVNK 260


>ref|XP_009606630.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana tomentosiformis]
           gi|697105623|ref|XP_009606631.1| PREDICTED:
           non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana tomentosiformis]
           gi|697105625|ref|XP_009606632.1| PREDICTED:
           non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana tomentosiformis]
           gi|697105627|ref|XP_009606633.1| PREDICTED:
           non-lysosomal glucosylceramidase-like isoform X3
           [Nicotiana tomentosiformis]
          Length = 947

 Score =  343 bits (880), Expect = 8e-92
 Identities = 170/259 (65%), Positives = 199/259 (76%), Gaps = 7/259 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P L W+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 2   LENGVDEEEGRDSLLQKVDPGKPPLLAWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 61

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 62  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 121

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 122 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDGEKCTYHAL 177

Query: 236 FPRAWTVYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLF 60
           +PRAWTVYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLF
Sbjct: 178 YPRAWTVYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLF 237

Query: 59  TWANSVGGDSGSTGGHLNE 3
           TWANSVGG S  +GGH+N+
Sbjct: 238 TWANSVGGISEFSGGHVNK 256


>ref|XP_009606627.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X1
           [Nicotiana tomentosiformis]
          Length = 972

 Score =  343 bits (880), Expect = 8e-92
 Identities = 170/259 (65%), Positives = 199/259 (76%), Gaps = 7/259 (2%)
 Frame = -1

Query: 758 MENGLEKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLF 597
           +ENG+++E   DS      P K P L W+RKLN S   P  ++  + E+LH+LPLG RL+
Sbjct: 27  LENGVDEEEGRDSLLQKVDPGKPPLLAWQRKLNCSGSTPTHFAPSIREILHLLPLGLRLW 86

Query: 596 RHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDT 417
           RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDT
Sbjct: 87  RHINEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDT 146

Query: 416 PILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHAL 237
           PILANQFS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL
Sbjct: 147 PILANQFSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDGEKCTYHAL 202

Query: 236 FPRAWTVYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLF 60
           +PRAWTVYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLF
Sbjct: 203 YPRAWTVYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLF 262

Query: 59  TWANSVGGDSGSTGGHLNE 3
           TWANSVGG S  +GGH+N+
Sbjct: 263 TWANSVGGISEFSGGHVNK 281


>ref|XP_006353596.1| PREDICTED: non-lysosomal glucosylceramidase-like [Solanum
           tuberosum]
          Length = 947

 Score =  337 bits (865), Expect = 5e-90
 Identities = 165/260 (63%), Positives = 196/260 (75%), Gaps = 9/260 (3%)
 Frame = -1

Query: 755 ENGLEKE---------IYSDSPKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWR 603
           ENG+++E         ++   P K P LTW+RKLN S+  P  ++  + E+LHMLPLG R
Sbjct: 3   ENGVDEEERPPCSDSLLHKVHPGKPPLLTWQRKLNSSASTPTSFAPSIREILHMLPLGLR 62

Query: 602 LFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCE 423
           L+RHI EEA KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CE
Sbjct: 63  LWRHIHEEAAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCE 122

Query: 422 DTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYH 243
           DTPILANQFS FI+RPD  + STVLCS  P    +LK +      ESW W LD E CTYH
Sbjct: 123 DTPILANQFSIFIARPDQEKFSTVLCSRGPE---ELKTD------ESWKWKLDGENCTYH 173

Query: 242 ALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLL 63
           AL+PRAWTVYDG+PDPE++I CRQ+SPF+PHNYK+SSFPV VFTFT+SNSGKT AD TLL
Sbjct: 174 ALYPRAWTVYDGVPDPELSIVCRQLSPFIPHNYKDSSFPVTVFTFTLSNSGKTDADVTLL 233

Query: 62  FTWANSVGGDSGSTGGHLNE 3
           FTWANSVGG S  +GGH+N+
Sbjct: 234 FTWANSVGGISEFSGGHVNK 253


>ref|XP_010313056.1| PREDICTED: LOW QUALITY PROTEIN: non-lysosomal glucosylceramidase
           [Solanum lycopersicum]
          Length = 946

 Score =  336 bits (861), Expect = 1e-89
 Identities = 164/260 (63%), Positives = 195/260 (75%), Gaps = 9/260 (3%)
 Frame = -1

Query: 755 ENGLEKE---------IYSDSPKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWR 603
           ENG+E+E         ++   P K P LTW+RKLN S+  P  ++  + E+LHMLPLG R
Sbjct: 3   ENGVEEEDQPPCSESLLHKVYPGKPPLLTWQRKLNSSASTPTSFAPSIREILHMLPLGLR 62

Query: 602 LFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCE 423
           L +HI EEA KGTPS++DPY+KRL+SCYHGVPL        GRS +GEFQRFQLFPR CE
Sbjct: 63  LLKHIHEEAAKGTPSIMDPYNKRLISCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCE 122

Query: 422 DTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYH 243
           DTPILANQFS FI+RPD  + STVLCS  P    +LK +      ESW W LD E CTYH
Sbjct: 123 DTPILANQFSIFIARPDREKFSTVLCSRGPK---ELKTD------ESWKWKLDGENCTYH 173

Query: 242 ALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLL 63
           AL+PRAWTVYDG+PDPE++I CRQ+SPF+PHNYK+SSFPV VFTFT+SNSGKT AD TLL
Sbjct: 174 ALYPRAWTVYDGVPDPELSIVCRQLSPFIPHNYKDSSFPVTVFTFTLSNSGKTDADVTLL 233

Query: 62  FTWANSVGGDSGSTGGHLNE 3
           FTWANSVGG S  +GGH+N+
Sbjct: 234 FTWANSVGGISEFSGGHVNK 253


>ref|XP_009760264.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X5
           [Nicotiana sylvestris]
          Length = 935

 Score =  334 bits (857), Expect = 4e-89
 Identities = 167/253 (66%), Positives = 195/253 (77%), Gaps = 1/253 (0%)
 Frame = -1

Query: 758 MENGLEKEIYSDSPKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLFRHIKEE 579
           +ENG+++E   DS      L  KRKLN S   P  ++  + E+LH+LPLG RL+RHI EE
Sbjct: 2   LENGVDEEERRDS------LLEKRKLNCSGSTPTHFAPSIREILHLLPLGLRLWRHINEE 55

Query: 578 ADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDTPILANQ 399
           A KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDTPILANQ
Sbjct: 56  AAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDTPILANQ 115

Query: 398 FSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHALFPRAWT 219
           FS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL+PRAWT
Sbjct: 116 FSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDREKCTYHALYPRAWT 171

Query: 218 VYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFTWANSV 42
           VYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLFTWANSV
Sbjct: 172 VYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLFTWANSV 231

Query: 41  GGDSGSTGGHLNE 3
           GG S  +GGH+N+
Sbjct: 232 GGISEFSGGHVNK 244


>ref|XP_009760255.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X4
           [Nicotiana sylvestris]
          Length = 939

 Score =  334 bits (857), Expect = 4e-89
 Identities = 167/253 (66%), Positives = 195/253 (77%), Gaps = 1/253 (0%)
 Frame = -1

Query: 758 MENGLEKEIYSDSPKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLFRHIKEE 579
           +ENG+++E   DS      L  KRKLN S   P  ++  + E+LH+LPLG RL+RHI EE
Sbjct: 6   LENGVDEEERRDS------LLEKRKLNCSGSTPTHFAPSIREILHLLPLGLRLWRHINEE 59

Query: 578 ADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDTPILANQ 399
           A KGTPS++DPY+KRLLSCYHGVPL        GRS +GEFQRFQLFPR CEDTPILANQ
Sbjct: 60  AAKGTPSIMDPYNKRLLSCYHGVPLGGIGAGSIGRSLRGEFQRFQLFPRKCEDTPILANQ 119

Query: 398 FSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHALFPRAWT 219
           FS FISRP+G + STVLCS  P    +LK N  G GIESW W LD EKCTYHAL+PRAWT
Sbjct: 120 FSVFISRPNGEKFSTVLCSRGPE---ELKTN-TGLGIESWKWKLDREKCTYHALYPRAWT 175

Query: 218 VYD-GLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFTWANSV 42
           VYD G+PDPE++I CRQ+SPF+PHNYK+SS PV VFTFT+SNSGKT AD TLLFTWANSV
Sbjct: 176 VYDAGVPDPELSIVCRQLSPFIPHNYKDSSLPVTVFTFTLSNSGKTDADVTLLFTWANSV 235

Query: 41  GGDSGSTGGHLNE 3
           GG S  +GGH+N+
Sbjct: 236 GGISEFSGGHVNK 248


>ref|XP_010645881.1| PREDICTED: non-lysosomal glucosylceramidase [Vitis vinifera]
           gi|731436352|ref|XP_010645882.1| PREDICTED:
           non-lysosomal glucosylceramidase [Vitis vinifera]
           gi|731436354|ref|XP_010645883.1| PREDICTED:
           non-lysosomal glucosylceramidase [Vitis vinifera]
           gi|297741007|emb|CBI31319.3| unnamed protein product
           [Vitis vinifera]
          Length = 953

 Score =  313 bits (801), Expect = 1e-82
 Identities = 148/253 (58%), Positives = 190/253 (75%)
 Frame = -1

Query: 764 GEMENGLEKEIYSDSPKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLFRHIK 585
           G+ME+ ++   +  +P K   LTW+RKLN  +     +++++ E+ H+ PLG RL+ H+ 
Sbjct: 9   GDMEHPVKSSAHKVNPGKPASLTWQRKLNTKANTLTRFNLKLREIKHLAPLGVRLWHHVN 68

Query: 584 EEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDTPILA 405
            EA KG  S++DP+ KRL++ YHGVPL        GRS++GEFQR+QLFPR+CED+P+LA
Sbjct: 69  AEAAKGRISIIDPFSKRLVTSYHGVPLGGIGGGSIGRSYRGEFQRYQLFPRICEDSPVLA 128

Query: 404 NQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHALFPRA 225
           NQFS F+SRP+G++SSTVLC   P     LK + + +GI SWDWNLD E CTYHAL+PRA
Sbjct: 129 NQFSVFVSRPNGKKSSTVLCPRNPEV---LKGSAS-SGIGSWDWNLDGESCTYHALYPRA 184

Query: 224 WTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFTWANS 45
           WTVY+G PDPEI+I   QISPF+PHNYKESSFPV+VF FT+SNSGKTSAD TLLFTWANS
Sbjct: 185 WTVYEGEPDPEISIISSQISPFIPHNYKESSFPVSVFKFTLSNSGKTSADITLLFTWANS 244

Query: 44  VGGDSGSTGGHLN 6
           VGG S  +G H N
Sbjct: 245 VGGTSEFSGHHYN 257


>ref|XP_011036252.1| PREDICTED: non-lysosomal glucosylceramidase isoform X3 [Populus
           euphratica] gi|743880618|ref|XP_011036253.1| PREDICTED:
           non-lysosomal glucosylceramidase isoform X3 [Populus
           euphratica]
          Length = 859

 Score =  310 bits (794), Expect = 8e-82
 Identities = 149/262 (56%), Positives = 191/262 (72%), Gaps = 11/262 (4%)
 Frame = -1

Query: 758 MENGL-----EKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPL 612
           MENGL     E++++ D+      P K   LTWKR+LN +  +P E+ +   E+ HMLP+
Sbjct: 1   MENGLAAAEGEEKLFPDTHAQKVDPGKPASLTWKRQLNSNGKIPVEFGLSFREISHMLPI 60

Query: 611 GWRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPR 432
           G RL+R++K+EA KG  ++ D   K +L+  HG+PL        GRS++GEFQ F+LFP 
Sbjct: 61  GLRLWRYVKQEAAKGRTAIFDFSKKHILTSDHGIPLGGIGAGSIGRSYRGEFQHFRLFPG 120

Query: 431 VCEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKC 252
           +CE+ P+LANQFSAF+SRP+G + S+VLCS T      +     G+GIESWDWNL+ +KC
Sbjct: 121 ICEEGPVLANQFSAFVSRPNGEKFSSVLCSRT----SDIPKESTGSGIESWDWNLNGQKC 176

Query: 251 TYHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADA 72
           TYHALFPRAWT+YDG PDPE+TI  RQISPF+PHNYKESSFPV+VFTFT+SN GKTSAD 
Sbjct: 177 TYHALFPRAWTIYDGAPDPELTIVSRQISPFIPHNYKESSFPVSVFTFTLSNHGKTSADV 236

Query: 71  TLLFTWANSVGGDSGSTGGHLN 6
           TL+FTWANSVGG SG +G H N
Sbjct: 237 TLMFTWANSVGGVSGLSGHHFN 258


>ref|XP_011036249.1| PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Populus
           euphratica] gi|743880608|ref|XP_011036250.1| PREDICTED:
           non-lysosomal glucosylceramidase isoform X1 [Populus
           euphratica]
          Length = 947

 Score =  310 bits (794), Expect = 8e-82
 Identities = 149/262 (56%), Positives = 191/262 (72%), Gaps = 11/262 (4%)
 Frame = -1

Query: 758 MENGL-----EKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPL 612
           MENGL     E++++ D+      P K   LTWKR+LN +  +P E+ +   E+ HMLP+
Sbjct: 1   MENGLAAAEGEEKLFPDTHAQKVDPGKPASLTWKRQLNSNGKIPVEFGLSFREISHMLPI 60

Query: 611 GWRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPR 432
           G RL+R++K+EA KG  ++ D   K +L+  HG+PL        GRS++GEFQ F+LFP 
Sbjct: 61  GLRLWRYVKQEAAKGRTAIFDFSKKHILTSDHGIPLGGIGAGSIGRSYRGEFQHFRLFPG 120

Query: 431 VCEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKC 252
           +CE+ P+LANQFSAF+SRP+G + S+VLCS T      +     G+GIESWDWNL+ +KC
Sbjct: 121 ICEEGPVLANQFSAFVSRPNGEKFSSVLCSRT----SDIPKESTGSGIESWDWNLNGQKC 176

Query: 251 TYHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADA 72
           TYHALFPRAWT+YDG PDPE+TI  RQISPF+PHNYKESSFPV+VFTFT+SN GKTSAD 
Sbjct: 177 TYHALFPRAWTIYDGAPDPELTIVSRQISPFIPHNYKESSFPVSVFTFTLSNHGKTSADV 236

Query: 71  TLLFTWANSVGGDSGSTGGHLN 6
           TL+FTWANSVGG SG +G H N
Sbjct: 237 TLMFTWANSVGGVSGLSGHHFN 258


>ref|XP_002325943.2| hypothetical protein POPTR_0019s10160g [Populus trichocarpa]
           gi|550317191|gb|EEF00325.2| hypothetical protein
           POPTR_0019s10160g [Populus trichocarpa]
          Length = 947

 Score =  310 bits (794), Expect = 8e-82
 Identities = 150/262 (57%), Positives = 189/262 (72%), Gaps = 11/262 (4%)
 Frame = -1

Query: 758 MENGL-----EKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPL 612
           MENGL     E++++ D+      P K   LTWKR+LN +  +P ++ +   E+ HMLP+
Sbjct: 1   MENGLAAAEGEEKLFPDTHAQKVDPGKPASLTWKRQLNSNGKIPVQFGLSFREISHMLPM 60

Query: 611 GWRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPR 432
           G RL+RHIK+EA K   ++ D   K +L+  HG+PL        GRS++GEFQ F+LFP 
Sbjct: 61  GLRLWRHIKQEAAKERATIFDFSKKHILTSDHGIPLGGIGAGSIGRSYRGEFQHFRLFPG 120

Query: 431 VCEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKC 252
           +CE+ P+LANQFSAF+SRP G   S+VLCS TP     +     G+GIESWDWNL+ +KC
Sbjct: 121 ICEEGPVLANQFSAFVSRPSGETFSSVLCSRTP----DIPKESTGSGIESWDWNLNGQKC 176

Query: 251 TYHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADA 72
           TYHALFPRAWT+YDG PDPE+TI  RQISPF+PHNYKESSFPV+VFTFT+SN GKTSAD 
Sbjct: 177 TYHALFPRAWTIYDGAPDPELTIVSRQISPFIPHNYKESSFPVSVFTFTLSNHGKTSADV 236

Query: 71  TLLFTWANSVGGDSGSTGGHLN 6
           TL+FTWANSVGG SG +G H N
Sbjct: 237 TLMFTWANSVGGVSGLSGHHFN 258


>ref|XP_011036251.1| PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Populus
           euphratica]
          Length = 947

 Score =  310 bits (793), Expect = 1e-81
 Identities = 149/262 (56%), Positives = 191/262 (72%), Gaps = 11/262 (4%)
 Frame = -1

Query: 758 MENGL-----EKEIYSDS------PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPL 612
           MENGL     E++++ D+      P K   LTWKR+LN +  +P E+ +   E+ HMLP+
Sbjct: 1   MENGLAAAEGEEKLFPDTHAQKVDPGKPASLTWKRQLNSNGKIPVEFGLSFREISHMLPI 60

Query: 611 GWRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPR 432
           G RL+R++K+EA KG  ++ D   K +L+  HG+PL        GRS++GEFQ F+LFP 
Sbjct: 61  GLRLWRYVKQEAAKGRVTIFDFSKKHILTSDHGIPLGGIGAGSIGRSYRGEFQHFRLFPG 120

Query: 431 VCEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKC 252
           +CE+ P+LANQFSAF+SRP+G + S+VLCS T      +     G+GIESWDWNL+ +KC
Sbjct: 121 ICEEGPVLANQFSAFVSRPNGEKFSSVLCSRT----SDIPKESTGSGIESWDWNLNGQKC 176

Query: 251 TYHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADA 72
           TYHALFPRAWT+YDG PDPE+TI  RQISPF+PHNYKESSFPV+VFTFT+SN GKTSAD 
Sbjct: 177 TYHALFPRAWTIYDGAPDPELTIVSRQISPFIPHNYKESSFPVSVFTFTLSNHGKTSADV 236

Query: 71  TLLFTWANSVGGDSGSTGGHLN 6
           TL+FTWANSVGG SG +G H N
Sbjct: 237 TLMFTWANSVGGVSGLSGHHFN 258


>ref|XP_007019883.1| Beta-glucosidase, GBA2 type family protein isoform 3 [Theobroma
           cacao] gi|508725211|gb|EOY17108.1| Beta-glucosidase,
           GBA2 type family protein isoform 3 [Theobroma cacao]
          Length = 758

 Score =  302 bits (773), Expect = 2e-79
 Identities = 144/261 (55%), Positives = 185/261 (70%), Gaps = 10/261 (3%)
 Frame = -1

Query: 758 MENGLEKEIYSDSPK----------KVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLG 609
           MENGL++ + +D             K P LTW+R+LN     P  + +   E++HM  +G
Sbjct: 1   MENGLKETVENDKASNGTLLEADSGKPPSLTWQRQLNSIRKPPTAFGLGFNEIIHMAQIG 60

Query: 608 WRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRV 429
           +RL+R+ KEE  KG  S+ D + K L++  HGVPL        GR ++GEFQRF+LFP+V
Sbjct: 61  FRLWRYTKEEEAKGRASIFDIFRKHLVTSDHGVPLGGIGAGSIGRGYRGEFQRFKLFPKV 120

Query: 428 CEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCT 249
           CE+ PILANQFSA +SRP+G + STVLC+ +P    ++     G GIESWDWNL  EKCT
Sbjct: 121 CEEGPILANQFSAIVSRPNGNKCSTVLCARSP----EVPKESTGLGIESWDWNLKGEKCT 176

Query: 248 YHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADAT 69
           YHALFPR+WT+Y+G PDPE+ I+C QISPF+PHNYKESSFPV+VFTFT+SN+G TSAD T
Sbjct: 177 YHALFPRSWTIYEGQPDPELRISCCQISPFIPHNYKESSFPVSVFTFTLSNTGSTSADVT 236

Query: 68  LLFTWANSVGGDSGSTGGHLN 6
           LLFTW NSVGG+SG +G H N
Sbjct: 237 LLFTWTNSVGGNSGFSGDHFN 257


>ref|XP_007019882.1| Beta-glucosidase, GBA2 type family protein isoform 2, partial
           [Theobroma cacao] gi|508725210|gb|EOY17107.1|
           Beta-glucosidase, GBA2 type family protein isoform 2,
           partial [Theobroma cacao]
          Length = 860

 Score =  302 bits (773), Expect = 2e-79
 Identities = 144/261 (55%), Positives = 185/261 (70%), Gaps = 10/261 (3%)
 Frame = -1

Query: 758 MENGLEKEIYSDSPK----------KVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLG 609
           MENGL++ + +D             K P LTW+R+LN     P  + +   E++HM  +G
Sbjct: 1   MENGLKETVENDKASNGTLLEADSGKPPSLTWQRQLNSIRKPPTAFGLGFNEIIHMAQIG 60

Query: 608 WRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRV 429
           +RL+R+ KEE  KG  S+ D + K L++  HGVPL        GR ++GEFQRF+LFP+V
Sbjct: 61  FRLWRYTKEEEAKGRASIFDIFRKHLVTSDHGVPLGGIGAGSIGRGYRGEFQRFKLFPKV 120

Query: 428 CEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCT 249
           CE+ PILANQFSA +SRP+G + STVLC+ +P    ++     G GIESWDWNL  EKCT
Sbjct: 121 CEEGPILANQFSAIVSRPNGNKCSTVLCARSP----EVPKESTGLGIESWDWNLKGEKCT 176

Query: 248 YHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADAT 69
           YHALFPR+WT+Y+G PDPE+ I+C QISPF+PHNYKESSFPV+VFTFT+SN+G TSAD T
Sbjct: 177 YHALFPRSWTIYEGQPDPELRISCCQISPFIPHNYKESSFPVSVFTFTLSNTGSTSADVT 236

Query: 68  LLFTWANSVGGDSGSTGGHLN 6
           LLFTW NSVGG+SG +G H N
Sbjct: 237 LLFTWTNSVGGNSGFSGDHFN 257


>ref|XP_007019881.1| Non-lysosomal glucosylceramidase isoform 1 [Theobroma cacao]
           gi|508725209|gb|EOY17106.1| Non-lysosomal
           glucosylceramidase isoform 1 [Theobroma cacao]
          Length = 948

 Score =  302 bits (773), Expect = 2e-79
 Identities = 144/261 (55%), Positives = 185/261 (70%), Gaps = 10/261 (3%)
 Frame = -1

Query: 758 MENGLEKEIYSDSPK----------KVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLG 609
           MENGL++ + +D             K P LTW+R+LN     P  + +   E++HM  +G
Sbjct: 1   MENGLKETVENDKASNGTLLEADSGKPPSLTWQRQLNSIRKPPTAFGLGFNEIIHMAQIG 60

Query: 608 WRLFRHIKEEADKGTPSVVDPYHKRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRV 429
           +RL+R+ KEE  KG  S+ D + K L++  HGVPL        GR ++GEFQRF+LFP+V
Sbjct: 61  FRLWRYTKEEEAKGRASIFDIFRKHLVTSDHGVPLGGIGAGSIGRGYRGEFQRFKLFPKV 120

Query: 428 CEDTPILANQFSAFISRPDGRQSSTVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCT 249
           CE+ PILANQFSA +SRP+G + STVLC+ +P    ++     G GIESWDWNL  EKCT
Sbjct: 121 CEEGPILANQFSAIVSRPNGNKCSTVLCARSP----EVPKESTGLGIESWDWNLKGEKCT 176

Query: 248 YHALFPRAWTVYDGLPDPEITITCRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADAT 69
           YHALFPR+WT+Y+G PDPE+ I+C QISPF+PHNYKESSFPV+VFTFT+SN+G TSAD T
Sbjct: 177 YHALFPRSWTIYEGQPDPELRISCCQISPFIPHNYKESSFPVSVFTFTLSNTGSTSADVT 236

Query: 68  LLFTWANSVGGDSGSTGGHLN 6
           LLFTW NSVGG+SG +G H N
Sbjct: 237 LLFTWTNSVGGNSGFSGDHFN 257


>ref|XP_010246140.1| PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Nelumbo
           nucifera]
          Length = 760

 Score =  298 bits (762), Expect = 4e-78
 Identities = 136/238 (57%), Positives = 183/238 (76%)
 Frame = -1

Query: 719 PKKVPELTWKRKLNHSSPLPKEYSVRVGEVLHMLPLGWRLFRHIKEEADKGTPSVVDPYH 540
           P +   LTW+RK+N  + +  E+++ + E++H+ P+G+R++R++K+EA KG   ++DP+ 
Sbjct: 25  PAQPALLTWQRKVNSQANVLSEFNLTIQEIIHLAPMGFRIWRYLKQEASKGRAIIMDPFK 84

Query: 539 KRLLSCYHGVPLXXXXXXXXGRSFKGEFQRFQLFPRVCEDTPILANQFSAFISRPDGRQS 360
           KR ++  HGVPL        GRS++GEFQR+QLFP +CE+ P+LANQFSAF+SR +G+  
Sbjct: 85  KRFVTSCHGVPLGGIGAGSIGRSYRGEFQRWQLFPGICEEKPVLANQFSAFVSRENGKSF 144

Query: 359 STVLCSTTPTAVGQLKNNVNGNGIESWDWNLDAEKCTYHALFPRAWTVYDGLPDPEITIT 180
           STVLC   P    Q+++N++G G  SWDWNL+ +  TYHALFPR+WTVYDG PDPE+ I 
Sbjct: 145 STVLCPKNPEL--QMESNISGIG--SWDWNLNGQSSTYHALFPRSWTVYDGEPDPELRIV 200

Query: 179 CRQISPFLPHNYKESSFPVAVFTFTVSNSGKTSADATLLFTWANSVGGDSGSTGGHLN 6
           CRQISPF+PHNYKESSFPVAVFTFT+ NSG+T+AD TLLFTW NSVGGDSG +G H N
Sbjct: 201 CRQISPFIPHNYKESSFPVAVFTFTLFNSGETAADITLLFTWTNSVGGDSGFSGYHFN 258