BLASTX nr result
ID: Papaver32_contig00034720
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00034720
(715 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AOR51553.1 putative N-methyltransferase [Papaver somniferum] 284 e-102
ANY58201.1 putative N-methyltransferase [synthetic construct] 181 7e-67
ANY58193.1 putative N-methyltransferase [synthetic construct] 180 6e-66
ANY58195.1 TNMT [synthetic construct] 171 9e-64
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 171 9e-64
ANY58190.1 putative N-methyltransferase [synthetic construct] 171 2e-63
ANY58191.1 putative N-methyltransferase [synthetic construct] 168 3e-63
ANY58196.1 putative N-methyltransferase [synthetic construct] 176 3e-62
ANY58200.1 putative N-methyltransferase [synthetic construct] 166 3e-61
ANY58192.1 putative N-methyltransferase [synthetic construct] 157 3e-59
C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine N-m... 155 8e-59
Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 157 2e-58
ANY58198.1 putative N-methyltransferase [synthetic construct] 154 1e-57
C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 158 1e-57
ANY58202.1 putative N-methyltransferase [synthetic construct] 144 1e-55
AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexan... 149 5e-51
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica] 148 5e-51
XP_019161911.1 PREDICTED: (S)-coclaurine N-methyltransferase-lik... 147 3e-49
ANY58194.1 putative N-methyltransferase [synthetic construct] 144 5e-49
XP_006474450.1 PREDICTED: (S)-coclaurine N-methyltransferase iso... 145 1e-48
>AOR51553.1 putative N-methyltransferase [Papaver somniferum]
Length = 356
Score = 284 bits (727), Expect(2) = e-102
Identities = 136/138 (98%), Positives = 137/138 (99%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV
Sbjct: 219 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 278
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
DQWLLSGTHMARSQEEWFKRFKINLEAASKALT GLGSEEAANQVNIQYKTFFMGYVVQF
Sbjct: 279 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTVGLGSEEAANQVNIQYKTFFMGYVVQF 338
Query: 64 SYNNGEEWMISHYLFKKK 11
S+NNGEEWMISHYLFKKK
Sbjct: 339 SFNNGEEWMISHYLFKKK 356
Score = 115 bits (287), Expect(2) = e-102
Identities = 57/60 (95%), Positives = 58/60 (96%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQTILDIGCGFGGLVLHIAQKYKN HVTGLTNSAEQRNYI LQVEKL+LSNVDVILADVT
Sbjct: 125 GQTILDIGCGFGGLVLHIAQKYKNSHVTGLTNSAEQRNYIMLQVEKLSLSNVDVILADVT 184
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 181 bits (459), Expect(2) = 7e-67
Identities = 85/138 (61%), Positives = 105/138 (76%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
W+K +DS LFV H+CHK FS HFE LDE+DWY+ ++ P+G VT SASALLYFQDDVSVV
Sbjct: 229 WLK-KDSLLFVDHICHKTFSHHFETLDEEDWYSGFIFPKGCVTLPSASALLYFQDDVSVV 287
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
D W+++G HMARS EEW K+ N+EAA + L GLGS+EA NQV +TF +G QF
Sbjct: 288 DHWVVNGMHMARSVEEWGKKLDKNMEAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQF 347
Query: 64 SYNNGEEWMISHYLFKKK 11
S+NNGEEWM++ LFKKK
Sbjct: 348 SFNNGEEWMVAQMLFKKK 365
Score = 101 bits (252), Expect(2) = 7e-67
Identities = 48/60 (80%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LD+GCG GGLVLHIAQKYKNC VTG+TNS Q NYI +Q EKL LSNVDVILADVT
Sbjct: 137 GQTVLDVGCGQGGLVLHIAQKYKNCRVTGITNSKAQMNYIVMQAEKLQLSNVDVILADVT 196
>ANY58193.1 putative N-methyltransferase [synthetic construct]
Length = 360
Score = 180 bits (456), Expect(2) = 6e-66
Identities = 85/138 (61%), Positives = 105/138 (76%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WMK +DS LFV H+ HK F+ HFEALDE+DWY+ ++ P+G VT LS+S LLYFQDDVSVV
Sbjct: 224 WMK-KDSLLFVDHISHKKFNHHFEALDEEDWYSGFIFPKGCVTILSSSTLLYFQDDVSVV 282
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
D W+++G HMARS EEW K+ N+EAA + L GLGS+EA NQV +TF +G QF
Sbjct: 283 DHWVVNGMHMARSMEEWRKKLDKNMEAAREILEPGLGSKEAVNQVITHIRTFCLGGYEQF 342
Query: 64 SYNNGEEWMISHYLFKKK 11
S+NNGEEWMI+ LFKKK
Sbjct: 343 SFNNGEEWMITQMLFKKK 360
Score = 99.8 bits (247), Expect(2) = 6e-66
Identities = 49/60 (81%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQTILDIGCG GGLVL+IAQKYKNCHVTGLTNS Q NYI +Q +KL LSNVDVI ADVT
Sbjct: 132 GQTILDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQMNYILMQAKKLQLSNVDVISADVT 191
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 171 bits (434), Expect(2) = 9e-64
Identities = 82/138 (59%), Positives = 101/138 (73%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM EDS LFV H+ HK F+ +FEALDEDDWY+ ++ P+G VT LS+S LLYFQDDVS +
Sbjct: 214 WMT-EDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSAL 272
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
D W+++G HMARS E W K+ +EAA + L GLGS+EA NQV +TF +G QF
Sbjct: 273 DHWVVNGMHMARSVEAWRKKLDNTIEAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQF 332
Query: 64 SYNNGEEWMISHYLFKKK 11
SYNNGEEWMI+ LFKKK
Sbjct: 333 SYNNGEEWMITQILFKKK 350
Score = 100 bits (250), Expect(2) = 9e-64
Identities = 48/60 (80%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IA+KYKNCHVTGLTNS Q NYIE Q EKL L+NVDVI ADVT
Sbjct: 122 GQTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELANVDVIFADVT 181
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 171 bits (434), Expect(2) = 9e-64
Identities = 82/138 (59%), Positives = 101/138 (73%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM EDS LFV H+ HK F+ +FEALDEDDWY+ ++ P+G VT LS+S LLYFQDDVS +
Sbjct: 214 WMT-EDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSAL 272
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
D W+++G HMARS E W K+ +EAA + L GLGS+EA NQV +TF +G QF
Sbjct: 273 DHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQF 332
Query: 64 SYNNGEEWMISHYLFKKK 11
SYNNGEEWMI+ LFKKK
Sbjct: 333 SYNNGEEWMITQILFKKK 350
Score = 100 bits (250), Expect(2) = 9e-64
Identities = 48/60 (80%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IA+KYKNCHVTGLTNS Q NYIE Q EKL L+NVDVI ADVT
Sbjct: 122 GQTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVT 181
>ANY58190.1 putative N-methyltransferase [synthetic construct]
Length = 362
Score = 171 bits (432), Expect(2) = 2e-63
Identities = 82/139 (58%), Positives = 101/139 (72%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM +DS LFV H+CHK FS FEA+DEDDWY+ ++ P G VT LS+ ALLYFQDDVSVV
Sbjct: 225 WMT-KDSLLFVDHICHKTFSHPFEAVDEDDWYSGFIFPPGCVTILSSDALLYFQDDVSVV 283
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G HMARS E W K+ N++AA + L GL G+ EA NQ+ +TF MG Q
Sbjct: 284 DHWVINGMHMARSVEAWRKKLDKNMKAAKEILLPGLGGNHEAVNQIVTHIRTFCMGGYEQ 343
Query: 67 FSYNNGEEWMISHYLFKKK 11
FSYNNGEEWM++ LFKKK
Sbjct: 344 FSYNNGEEWMVAQMLFKKK 362
Score = 100 bits (249), Expect(2) = 2e-63
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IA+KYK CHVTGLTNS Q NYIE+Q +KL LSNVDVILADVT
Sbjct: 133 GQTVLDIGCGQGGLVLYIAEKYKKCHVTGLTNSKAQVNYIEMQAKKLGLSNVDVILADVT 192
>ANY58191.1 putative N-methyltransferase [synthetic construct]
Length = 350
Score = 168 bits (426), Expect(2) = 3e-63
Identities = 79/139 (56%), Positives = 101/139 (72%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM +E S LFV H+CHK F+ HFEA+DE+DWY+ Y+ P+G VT LS+SALLYFQ DVSVV
Sbjct: 213 WMTEE-SLLFVDHICHKTFNHHFEAMDEEDWYSGYIFPKGCVTILSSSALLYFQSDVSVV 271
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G HMARS +EW K+ N+E L + GS+EA +QV +TF MG Q
Sbjct: 272 DHWVVNGNHMARSVQEWLKKLDDNMETVRGILEPAMGGSKEAVDQVITHSRTFCMGAYEQ 331
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS+NNGEEWM++ LFKKK
Sbjct: 332 FSFNNGEEWMVAQILFKKK 350
Score = 102 bits (253), Expect(2) = 3e-63
Identities = 48/60 (80%), Positives = 54/60 (90%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IAQKYKNCHVTGLTNS Q NYIE+Q +KLNL+NV+ ILADVT
Sbjct: 119 GQTVLDIGCGHGGLVLYIAQKYKNCHVTGLTNSRAQVNYIEMQSKKLNLTNVEAILADVT 178
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 176 bits (447), Expect(2) = 3e-62
Identities = 81/139 (58%), Positives = 102/139 (73%)
Frame = -1
Query: 427 KWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSV 248
KWMKD D +F+ +CHK F HFEA+DEDDWY+ Y+ P+GSVT +AS LLYFQ+DV+V
Sbjct: 220 KWMKD-DGLMFIDCICHKTFGHHFEAIDEDDWYSGYIFPKGSVTLQAASTLLYFQEDVAV 278
Query: 247 VDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQ 68
VDQW +SG HMAR+ EEW K+ N++ A + L LGS+EA +V +TF +G Q
Sbjct: 279 VDQWAVSGKHMARTVEEWLKKLDKNIDVAREILEPSLGSKEAVEKVITHSRTFCIGTSEQ 338
Query: 67 FSYNNGEEWMISHYLFKKK 11
FSYNNG+EWMISH LFKKK
Sbjct: 339 FSYNNGDEWMISHVLFKKK 357
Score = 90.9 bits (224), Expect(2) = 3e-62
Identities = 42/60 (70%), Positives = 49/60 (81%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQ +LDIGCG G L++HIAQKYKNCHVTG+TNS Q+N IE + L LSNV+VILADVT
Sbjct: 129 GQRVLDIGCGQGSLIIHIAQKYKNCHVTGITNSNAQKNIIEEECRNLKLSNVEVILADVT 188
>ANY58200.1 putative N-methyltransferase [synthetic construct]
Length = 358
Score = 166 bits (420), Expect(2) = 3e-61
Identities = 80/139 (57%), Positives = 100/139 (71%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM EDS LFV H+CHK FS FEA+DEDDWY+ ++ P GSVT L+A++LLYFQD+VSVV
Sbjct: 221 WMT-EDSLLFVEHVCHKTFSHFFEAIDEDDWYSGFIFPPGSVTILAANSLLYFQDEVSVV 279
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G H ARS + W K+ N+E A + L GL GS EA N V +TF MG Q
Sbjct: 280 DHWVVNGMHTARSFDAWRKKVDKNMEVAKEILLPGLGGSHEAVNAVVTHIRTFCMGGYEQ 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS NNG+EWM++H LFKKK
Sbjct: 340 FSLNNGDEWMVAHLLFKKK 358
Score = 97.8 bits (242), Expect(2) = 3e-61
Identities = 48/60 (80%), Positives = 51/60 (85%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IAQKYK CHVTGLTNS EQ NYI Q EKL L NVDV+LADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIAQKYKKCHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVT 188
>ANY58192.1 putative N-methyltransferase [synthetic construct]
Length = 353
Score = 157 bits (397), Expect(2) = 3e-59
Identities = 75/139 (53%), Positives = 98/139 (70%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM +E S LFV H+CHK F+ HFE +DE+DWY+ ++ PEG VT LS+S+LLYFQ DVSV+
Sbjct: 216 WMTEE-SLLFVDHICHKTFNYHFEPMDENDWYSGHIFPEGCVTILSSSSLLYFQKDVSVL 274
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G HMARS +EW K N+E + L + G++EA +QV Q + F MG Q
Sbjct: 275 DHWVVNGKHMARSAQEWLKSLDDNMEKVREILEPSMGGNKEAVDQVLTQSRFFCMGAYEQ 334
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS NNGEEWM + LFKKK
Sbjct: 335 FSNNNGEEWMFAQLLFKKK 353
Score = 100 bits (248), Expect(2) = 3e-59
Identities = 48/60 (80%), Positives = 54/60 (90%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IAQKYKNCHVTGLTNS Q +IELQ +KLNL+NV+VILADVT
Sbjct: 122 GQTVLDIGCGNGGLVLYIAQKYKNCHVTGLTNSKAQVKHIELQTKKLNLTNVEVILADVT 181
>C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 ACO90236.1 putative
N-methyltransferase [Papaver bracteatum] ANY58199.1
TNMT2 [synthetic construct]
Length = 358
Score = 155 bits (391), Expect(2) = 8e-59
Identities = 76/139 (54%), Positives = 95/139 (68%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM EDS LFV H CHK F+ FEALDEDDWY+ Y+ P G T+LSA +LLYFQDDVSVV
Sbjct: 221 WMA-EDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVV 279
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G H AR+ + W K+ N+EA + L GL G+ EA N V +T +G VQ
Sbjct: 280 DHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQ 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS N+G+EWM + LFKKK
Sbjct: 340 FSLNDGDEWMNAQLLFKKK 358
Score = 100 bits (250), Expect(2) = 8e-59
Identities = 49/60 (81%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL++AQKYKNCHVTGLTNS EQ NYI Q EKL L NVDVILADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVT 188
>Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT AAY79177.1
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 157 bits (397), Expect(2) = 2e-58
Identities = 77/139 (55%), Positives = 96/139 (69%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM E S LFV H+CHK F+ FEA+DEDDWY+ ++ P G T L+A++LLYFQDDVSVV
Sbjct: 221 WMTKE-SLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVV 279
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G HMARS + W K N+EAA + L GL GS E N V +TF MG Q
Sbjct: 280 DHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQ 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS NNG+EWM++ LFKKK
Sbjct: 340 FSMNNGDEWMVAQLLFKKK 358
Score = 97.4 bits (241), Expect(2) = 2e-58
Identities = 47/60 (78%), Positives = 51/60 (85%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IAQKYKNCHVTGLTNS Q NY+ Q EKL L+NVD ILADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVT 188
>ANY58198.1 putative N-methyltransferase [synthetic construct]
Length = 359
Score = 154 bits (389), Expect(2) = 1e-57
Identities = 76/139 (54%), Positives = 95/139 (68%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM EDS LFV H CHK F+ FEALDEDDWY+ Y+ P G T+LSA +LLYFQDDVSVV
Sbjct: 221 WMA-EDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVV 279
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G H AR+ + W K+ N+EA + L GL G+ EA N V KT +G VQ
Sbjct: 280 DHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIKTCCVGGYVQ 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS ++G+EWM + LFKKK
Sbjct: 340 FSLSDGDEWMNAQLLFKKK 358
Score = 97.8 bits (242), Expect(2) = 1e-57
Identities = 48/60 (80%), Positives = 51/60 (85%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IAQKYK CHVTGLTNS EQ NYI Q EKL L NVDV+LADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIAQKYKKCHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVT 188
>C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 ACO90237.1 tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 158 bits (399), Expect(2) = 1e-57
Identities = 77/139 (55%), Positives = 98/139 (70%), Gaps = 1/139 (0%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WM +E S LFV H+CHK F+ FEA+DEDDWY+ ++ P G T L+A++LLYFQDDVSVV
Sbjct: 221 WMTEE-SLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVV 279
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGL-GSEEAANQVNIQYKTFFMGYVVQ 68
D W+++G HMARS + W K N+EAA + L GL GS EA N V +TF MG Q
Sbjct: 280 DHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQ 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
FS N+G+EWM++ LFKKK
Sbjct: 340 FSMNDGDEWMVAQLLFKKK 358
Score = 93.6 bits (231), Expect(2) = 1e-57
Identities = 45/60 (75%), Positives = 50/60 (83%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQT+LDIGCG GGLVL+IA+KYK CHVTGLTNS Q NY+ Q EKL L+NVD ILADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVT 188
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 144 bits (362), Expect(2) = 1e-55
Identities = 68/111 (61%), Positives = 84/111 (75%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
WMK EDS LFV H+ HK F+ HFEALDE+DWY+ ++ P+G VT LS+S LLYFQDDVSVV
Sbjct: 224 WMK-EDSLLFVDHISHKTFNHHFEALDEEDWYSGFIFPKGCVTILSSSTLLYFQDDVSVV 282
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKT 92
D W+++G HMARS EEW K+ N+EAA + L GLGS+EA NQV +T
Sbjct: 283 DHWVVNGMHMARSVEEWRKKLDKNMEAAREILEPGLGSKEAVNQVITHIRT 333
Score = 101 bits (252), Expect(2) = 1e-55
Identities = 49/60 (81%), Positives = 52/60 (86%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQ++LDIGCG GGLVLHIAQKYKNCHVTGLTNS Q NYI +Q EKL LSNVDVI ADVT
Sbjct: 132 GQSVLDIGCGQGGLVLHIAQKYKNCHVTGLTNSKAQMNYILMQAEKLQLSNVDVISADVT 191
>AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
Length = 370
Score = 149 bits (377), Expect(2) = 5e-51
Identities = 70/139 (50%), Positives = 89/139 (64%)
Frame = -1
Query: 427 KWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSV 248
KWM +D LF+ H+CHK F+ H++ LDEDDW+T YV P G++ SAS LLYFQDDVSV
Sbjct: 233 KWMS-QDGLLFIEHICHKTFAYHYDPLDEDDWFTDYVFPAGTMIIPSASFLLYFQDDVSV 291
Query: 247 VDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQ 68
V+ W LSG H +R+ EEW KR N++ LGSEEAA ++ +K F +
Sbjct: 292 VNHWTLSGKHFSRTNEEWLKRLDANVDTIKAIFEASLGSEEAAVKLINYWKGFCFSGMEM 351
Query: 67 FSYNNGEEWMISHYLFKKK 11
F NNGEEWM SH L +K
Sbjct: 352 FGSNNGEEWMTSHLLLTRK 370
Score = 80.1 bits (196), Expect(2) = 5e-51
Identities = 36/60 (60%), Positives = 47/60 (78%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
G TILD+GCG G L LHIA+KY NC VT +TNS Q+++I+ Q K NL+NV+V+LAD+T
Sbjct: 142 GHTILDLGCGQGALTLHIAKKYTNCRVTAITNSVSQKHFIQDQCIKSNLTNVEVLLADIT 201
>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 148 bits (374), Expect(2) = 5e-51
Identities = 68/139 (48%), Positives = 91/139 (65%)
Frame = -1
Query: 427 KWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSV 248
+W+ +D LF+ H+CHK F+ H+E LD+DDW+T YV P G++ SAS LYFQDDVSV
Sbjct: 221 EWIS-KDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSV 279
Query: 247 VDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQ 68
V+ W LSG H +R+ EEW KR NL+ +G+EE A ++ ++ F + +
Sbjct: 280 VNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEM 339
Query: 67 FSYNNGEEWMISHYLFKKK 11
F YNNGEEWM SH LFKKK
Sbjct: 340 FGYNNGEEWMASHVLFKKK 358
Score = 81.3 bits (199), Expect(2) = 5e-51
Identities = 36/60 (60%), Positives = 46/60 (76%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
GQ++LD+GCG G L LH+AQKYKNC VT +TNS Q+ YIE + + NL NV+V LAD+T
Sbjct: 130 GQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADIT 189
>XP_019161911.1 PREDICTED: (S)-coclaurine N-methyltransferase-like [Ipomoea nil]
Length = 355
Score = 147 bits (371), Expect(2) = 3e-49
Identities = 69/139 (49%), Positives = 96/139 (69%)
Frame = -1
Query: 427 KWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSV 248
KWMK DSFLFV + CHK F+ HFE +++DDW T Y G++ SA+ LLYFQDDVSV
Sbjct: 221 KWMKP-DSFLFVHYFCHKTFAYHFEDINDDDWITRYFFSGGTMP--SANLLLYFQDDVSV 277
Query: 247 VDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQ 68
+D WL++G H AR+ EEW KR N+++ K + E +++A + + ++TFF+
Sbjct: 278 IDHWLVNGKHYARTSEEWLKRMDENIKSV-KPIMESTYGKDSALKWTVYWRTFFIAVAEL 336
Query: 67 FSYNNGEEWMISHYLFKKK 11
F YNNGEEWM++H+LFKKK
Sbjct: 337 FGYNNGEEWMVAHFLFKKK 355
Score = 76.6 bits (187), Expect(2) = 3e-49
Identities = 33/60 (55%), Positives = 44/60 (73%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
G T+LD+GCG+G L L+IA KY NC VTG+ NS Q+ +IE Q L L NVD+I+AD++
Sbjct: 130 GDTVLDVGCGWGSLSLYIANKYSNCKVTGICNSVTQKEHIEQQCRDLQLENVDIIVADIS 189
>ANY58194.1 putative N-methyltransferase [synthetic construct]
Length = 355
Score = 144 bits (364), Expect(2) = 5e-49
Identities = 70/138 (50%), Positives = 93/138 (67%)
Frame = -1
Query: 424 WMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSVV 245
W+K +DS LFV H CHKAF+ HFE EDDW T Y G++ SA+ LLYFQDDVSVV
Sbjct: 222 WLK-QDSLLFVHHFCHKAFAYHFEDTSEDDWITRYFFTGGTMP--SANLLLYFQDDVSVV 278
Query: 244 DQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQF 65
+ WL++G H A++ EEW KR NL + K + E +E+A + + ++TFF+ F
Sbjct: 279 NHWLVNGKHYAQTSEEWLKRMDKNLNSI-KPIMESTYGKESAVKWTVYWRTFFISVAELF 337
Query: 64 SYNNGEEWMISHYLFKKK 11
YNNGEEWM++H+LFKKK
Sbjct: 338 GYNNGEEWMVAHFLFKKK 355
Score = 78.6 bits (192), Expect(2) = 5e-49
Identities = 33/60 (55%), Positives = 48/60 (80%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
G T+LD+GCG+G L L+IAQKY NC +TG+ NS Q+ +IE Q +LNL+NV++I+AD++
Sbjct: 130 GHTVLDVGCGWGSLSLYIAQKYSNCKITGICNSVTQKAHIEEQSRELNLNNVEIIVADIS 189
>XP_006474450.1 PREDICTED: (S)-coclaurine N-methyltransferase isoform X1 [Citrus
sinensis] BAK61826.1 coclaurine N-methyltransferase
[Citrus unshiu] KDO73561.1 hypothetical protein
CISIN_1g018346mg [Citrus sinensis]
Length = 357
Score = 145 bits (367), Expect(2) = 1e-48
Identities = 70/139 (50%), Positives = 94/139 (67%)
Frame = -1
Query: 427 KWMKDEDSFLFVVHLCHKAFSQHFEALDEDDWYTSYVLPEGSVTYLSASALLYFQDDVSV 248
KWMK ED+ LFV H CHK F+ HFE ++DDW T Y G++ SA+ LLYFQDDVSV
Sbjct: 221 KWMK-EDTLLFVHHFCHKTFAYHFEDTNDDDWITKYFFTGGTMP--SANLLLYFQDDVSV 277
Query: 247 VDQWLLSGTHMARSQEEWFKRFKINLEAASKALTEGLGSEEAANQVNIQYKTFFMGYVVQ 68
VD WL++G H A++ EEW KR NL A+ K + E ++ A + + ++TFF+
Sbjct: 278 VDHWLVNGKHYAQTSEEWLKRMDNNL-ASIKPIMESTYGKDQAVKWTVYWRTFFIAVAEL 336
Query: 67 FSYNNGEEWMISHYLFKKK 11
F YNNGEEWM++H+LF+KK
Sbjct: 337 FGYNNGEEWMVTHFLFRKK 355
Score = 75.9 bits (185), Expect(2) = 1e-48
Identities = 32/60 (53%), Positives = 45/60 (75%)
Frame = -3
Query: 713 GQTILDIGCGFGGLVLHIAQKYKNCHVTGLTNSAEQRNYIELQVEKLNLSNVDVILADVT 534
G T+LD+GCG+G L L+IAQKY NC +TG+ NS Q+ +IE Q L L NV++I+AD++
Sbjct: 130 GHTVLDVGCGWGSLSLYIAQKYSNCKITGICNSKTQKEFIEEQCRVLELQNVEIIVADIS 189