BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver32_contig00034038
         (744 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AOR51552.1 reticuline N-methyltransferase [Papaver somniferum]        360   e-122
AFK73712.1 pavine-N-methyltransferase [Papaver somniferum]            304   e-100
5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4...   272   5e-87
C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNM...   270   8e-87
5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Compl...   268   2e-85
Q7XB08.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short...   265   7e-85
AKH61489.1 N-methyltransferase [Expression vector pWCD2353]           265   7e-85
AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexan...   251   5e-79
ACO90253.1 putative N-methyltransferase [Thalictrum flavum]           246   2e-77
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]           246   3e-77
Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short...   245   9e-77
ANY58196.1 putative N-methyltransferase [synthetic construct]         235   5e-73
ANY58193.1 putative N-methyltransferase [synthetic construct]         224   1e-68
ANY58202.1 putative N-methyltransferase [synthetic construct]         222   3e-68
ANY58201.1 putative N-methyltransferase [synthetic construct]         218   3e-66
ANY58195.1 TNMT [synthetic construct]                                 216   1e-65
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran...   216   1e-65
XP_008220523.1 PREDICTED: (S)-coclaurine N-methyltransferase iso...   215   3e-65
ANY58198.1 putative N-methyltransferase [synthetic construct]         215   3e-65
ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata]    215   4e-65

>AOR51552.1 reticuline N-methyltransferase [Papaver somniferum]
          Length = 363

 Score =  360 bits (923), Expect = e-122
 Identities = 181/227 (79%), Positives = 203/227 (89%), Gaps = 2/227 (0%)
 Frame = +3

Query: 69  TTTMDTTKISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFN 248
           +TTM+TTKISQQDDLWKNMELG I DEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLL+FN
Sbjct: 2   STTMETTKISQQDDLWKNMELGQISDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLDFN 61

Query: 249 KSLRGMKMATELDKLDS-KLYETPHSFNQIVNGSTLKESSGLYTD-VTTTIDEASIKMMD 422
           KSLRGMKMATE+D L++ K+YETP SFNQI+ G   KES+GL+TD  TTT++EA+ KMMD
Sbjct: 62  KSLRGMKMATEIDTLENHKIYETPESFNQIIGG---KESAGLFTDETTTTMEEANTKMMD 118

Query: 423 LYCERANIKDGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLS 602
           LYCERA +KDG TILDLGCG G LVLH+AKKY   KITG+TN  S KEYIL++CK L+LS
Sbjct: 119 LYCERAGLKDGHTILDLGCGAGLLVLHLAKKYKKSKITGITNTSSHKEYILKQCKNLNLS 178

Query: 603 NVEIILADVTSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           NVEIILADVT +D+E+TFDRVFVIGLIEHMKNFELFLRKISKWMKDD
Sbjct: 179 NVEIILADVTKVDIESTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 225


>AFK73712.1 pavine-N-methyltransferase [Papaver somniferum]
          Length = 330

 Score =  304 bits (779), Expect = e-100
 Identities = 153/195 (78%), Positives = 173/195 (88%), Gaps = 2/195 (1%)
 Frame = +3

Query: 165 MKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELDKLDS-KLYETPHSFNQIVN 341
           MKIGIEKRIKWGTKPTQQEQLAQLL+FNKSLRGMKMATE+D L++ K+YETP SFNQI+ 
Sbjct: 1   MKIGIEKRIKWGTKPTQQEQLAQLLDFNKSLRGMKMATEIDTLENHKIYETPESFNQIIG 60

Query: 342 GSTLKESSGLYTD-VTTTIDEASIKMMDLYCERANIKDGQTILDLGCGPGPLVLHIAKKY 518
           G   KES+GL+TD  TTT++EA+ KMMDLYCERA +KDG TILDLGCG G LVLH+AKKY
Sbjct: 61  G---KESAGLFTDETTTTMEEANTKMMDLYCERAGLKDGHTILDLGCGAGLLVLHLAKKY 117

Query: 519 SNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDMETTFDRVFVIGLIEHMKN 698
              KITG+TN  S KEYIL++CK L+LSNVEIILADVT +D+E+TFDRVFVIGLIEHMKN
Sbjct: 118 KKSKITGITNTSSHKEYILKQCKNLNLSNVEIILADVTKVDIESTFDRVFVIGLIEHMKN 177

Query: 699 FELFLRKISKWMKDD 743
           FELFLRKISKWMKDD
Sbjct: 178 FELFLRKISKWMKDD 192


>5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4_B Chain
           B, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KOC_A
           Chain A, Pavine N-methyltransferase In Complex With
           S-adenosylmethionine Ph 7 5KOC_B Chain B, Pavine
           N-methyltransferase In Complex With S-adenosylmethionine
           Ph 7 5KOK_A Chain A, Pavine N-methyltransferase In
           Complex With Tetrahydropapaverine And S-
           Adenosylhomocysteine Ph 7.25 5KOK_B Chain B, Pavine
           N-methyltransferase In Complex With Tetrahydropapaverine
           And S- Adenosylhomocysteine Ph 7.25 5KPG_A Chain A,
           Pavine N-methyltransferase In Complex With
           S-adenosylhomocysteine Ph 7 5KPG_B Chain B, Pavine
           N-methyltransferase In Complex With
           S-adenosylhomocysteine Ph 7
          Length = 397

 Score =  272 bits (696), Expect = 5e-87
 Identities = 128/219 (58%), Positives = 169/219 (77%)
 Frame = +3

Query: 87  TKISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGM 266
           TK     +L K +E G + DEE+R +MKI ++KR+KWG KPT ++QLAQL+ F +SL+GM
Sbjct: 47  TKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGM 106

Query: 267 KMATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANI 446
           +MA E+D LD++LYE P  F  I+ G TLK S G + D +TT+DE+ + MMDLYCERA I
Sbjct: 107 EMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQI 166

Query: 447 KDGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILAD 626
           KDGQ+ILDLGCG G L LH+A+KY  CK+TG+TN+ SQKE+I+++CKKL LSNVEIIL D
Sbjct: 167 KDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILED 226

Query: 627 VTSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           VT  + E T+DR+F + LIEHMKN+ELFL+K+S W+  D
Sbjct: 227 VTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQD 265


>C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT ACO90251.1
           pavine N-methyltransferase [Thalictrum flavum]
           ANY58204.1 PNMT [synthetic construct]
          Length = 356

 Score =  270 bits (691), Expect = 8e-87
 Identities = 127/216 (58%), Positives = 168/216 (77%)
 Frame = +3

Query: 87  TKISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGM 266
           TK     +L K +E G + DEE+R +MKI ++KR+KWG KPT ++QLAQL+ F +SL+GM
Sbjct: 6   TKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGM 65

Query: 267 KMATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANI 446
           +MA E+D LD++LYE P  F  I+ G TLK S G + D +TT+DE+ + MMDLYCERA I
Sbjct: 66  EMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQI 125

Query: 447 KDGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILAD 626
           KDGQ+ILDLGCG G L LH+A+KY  CK+TG+TN+ SQKE+I+++CKKL LSNVEIIL D
Sbjct: 126 KDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILED 185

Query: 627 VTSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWM 734
           VT  + E T+DR+F + LIEHMKN+ELFL+K+S W+
Sbjct: 186 VTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWI 221


>5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Complex With S-
           Adenosylmethionine Ph 6 5KPC_B Chain B, Pavine
           N-methyltransferase H206a Mutant In Complex With S-
           Adenosylmethionine Ph 6
          Length = 397

 Score =  268 bits (686), Expect = 2e-85
 Identities = 127/219 (57%), Positives = 168/219 (76%)
 Frame = +3

Query: 87  TKISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGM 266
           TK     +L K +E G + DEE+R +MKI ++KR+KWG KPT ++QLAQL+ F +SL+GM
Sbjct: 47  TKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGM 106

Query: 267 KMATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANI 446
           +MA E+D LD++LYE P  F  I+ G TLK S G + D +TT+DE+ + MMDLYCERA I
Sbjct: 107 EMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQI 166

Query: 447 KDGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILAD 626
           KDGQ+ILDLGCG G L LH+A+KY  CK+TG+TN+ SQKE+I+++CKKL LSNVEIIL D
Sbjct: 167 KDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILED 226

Query: 627 VTSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           VT  + E T+DR+F + LIE MKN+ELFL+K+S W+  D
Sbjct: 227 VTKFETEITYDRIFAVALIEAMKNYELFLKKVSTWIAQD 265


>Q7XB08.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=PsCNMT
           AAP45316.1 S-adenosyl-L-methionine:coclaurine
           N-methyltransferase [Papaver somniferum] AHF27396.1
           S-adenosyl-L-methionine:coclaurine N-methyltransferase
           [synthetic construct]
          Length = 351

 Score =  265 bits (678), Expect = 7e-85
 Identities = 121/218 (55%), Positives = 173/218 (79%)
 Frame = +3

Query: 90  KISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMK 269
           ++  +++L +NMELGLIPD+E+R+L+++ +EKR++WG K T +EQL+QLL+   SL+GMK
Sbjct: 2   QLKAKEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMK 61

Query: 270 MATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIK 449
           MATE++ LD KLYE P  F +I +GS +K+S+G YTD +TT+DEA I M+DLY ERA IK
Sbjct: 62  MATEMENLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIK 121

Query: 450 DGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADV 629
           DGQ++LDLGCG G + L  A K+  C+ TGVT++  QK+YI  +CK+L L+NV+++LAD+
Sbjct: 122 DGQSVLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADI 181

Query: 630 TSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           T+ + E  FDR+F + LIEHMKN++L L+KIS+WMKDD
Sbjct: 182 TTYETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDD 219


>AKH61489.1 N-methyltransferase [Expression vector pWCD2353]
          Length = 353

 Score =  265 bits (678), Expect = 7e-85
 Identities = 121/218 (55%), Positives = 173/218 (79%)
 Frame = +3

Query: 90  KISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMK 269
           ++  +++L +NMELGLIPD+E+R+L+++ +EKR++WG K T +EQL+QLL+   SL+GMK
Sbjct: 2   QLKAKEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMK 61

Query: 270 MATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIK 449
           MATE++ LD KLYE P  F +I +GS +K+S+G YTD +TT+DEA I M+DLY ERA IK
Sbjct: 62  MATEMENLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIK 121

Query: 450 DGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADV 629
           DGQ++LDLGCG G + L  A K+  C+ TGVT++  QK+YI  +CK+L L+NV+++LAD+
Sbjct: 122 DGQSVLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADI 181

Query: 630 TSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           T+ + E  FDR+F + LIEHMKN++L L+KIS+WMKDD
Sbjct: 182 TTYETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDD 219


>AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
          Length = 370

 Score =  251 bits (641), Expect = 5e-79
 Identities = 116/212 (54%), Positives = 162/212 (76%)
 Frame = +3

Query: 108 DLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELD 287
           +L + +ELG++PD E+++L++  +++R++WG K T Q+QLAQL++F  SLR M +A E+D
Sbjct: 27  ELLRKLELGVVPDHEIKQLIRHELDRRLRWGYKSTHQQQLAQLVDFAHSLRRMNIADEVD 86

Query: 288 KLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQTIL 467
            LDS +YE P SF +I+NG  LK S   +   +TT+ EA I M+DLYCERA ++DG TIL
Sbjct: 87  TLDSNIYEVPISFLRIMNGPMLKGSGCYFPHDSTTLAEAEIAMLDLYCERAQVRDGHTIL 146

Query: 468 DLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDME 647
           DLGCG G L LHIAKKY+NC++T +TN+ SQK +I ++C K +L+NVE++LAD+T  D  
Sbjct: 147 DLGCGQGALTLHIAKKYTNCRVTAITNSVSQKHFIQDQCIKSNLTNVEVLLADITKHDTS 206

Query: 648 TTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            +FDRVFVI L+EHMKN+EL L+KISKWM  D
Sbjct: 207 HSFDRVFVIELLEHMKNYELLLKKISKWMSQD 238


>ACO90253.1 putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  246 bits (629), Expect = 2e-77
 Identities = 115/217 (52%), Positives = 163/217 (75%)
 Frame = +3

Query: 93  ISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKM 272
           +  + +L+K +ELGLIP++EV++LM+I + +R++WG K T +EQ+AQ+L+   SLR M +
Sbjct: 3   VGGKQELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNI 62

Query: 273 ATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKD 452
           A E+D LDS +YE P  F +I+NGS LK SS  + D +TT+DEA I MMDL+CERA IKD
Sbjct: 63  AMEVDTLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKD 122

Query: 453 GQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVT 632
           G ++LDLGCG G L  ++A+KY    +T VTN+ SQKEYI EE ++ +LSNVE++LAD+ 
Sbjct: 123 GHSVLDLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIA 182

Query: 633 SLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           + +M  T+DR+ V+GL EHMKN+ L L+KIS+WM  D
Sbjct: 183 THEMADTYDRILVVGLFEHMKNYGLLLKKISEWMAKD 219


>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  246 bits (628), Expect = 3e-77
 Identities = 116/219 (52%), Positives = 166/219 (75%)
 Frame = +3

Query: 87  TKISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGM 266
           TK +   +L K +ELGL+P +++++L++  + +R++WG KPT +EQ+A++     SLR M
Sbjct: 8   TKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQM 67

Query: 267 KMATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANI 446
           K+ATE++ LDS+LYE P  F +I+NGS LK S   + + +TT+DEA I M+DLYCERA I
Sbjct: 68  KIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQI 127

Query: 447 KDGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILAD 626
           +DGQ++LDLGCG G L LH+A+KY NC++T VTN+ SQKEYI EE ++ +L NVE+ LAD
Sbjct: 128 QDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLAD 187

Query: 627 VTSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           +T+ +M  T+DR+ VI L EHMKN+EL LRKIS+W+  D
Sbjct: 188 ITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKD 226


>Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           AAU20766.1 (S)-coclaurine N-methyltransferase
           [Thalictrum flavum subsp. glaucum] ANY58203.1 CNMT
           [synthetic construct]
          Length = 361

 Score =  245 bits (625), Expect = 9e-77
 Identities = 113/212 (53%), Positives = 162/212 (76%)
 Frame = +3

Query: 108 DLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELD 287
           +L K +ELGL+PD+E+++L++I + +R++WG K T +EQ+AQL+    SLR MK+ATE++
Sbjct: 17  ELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVE 76

Query: 288 KLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQTIL 467
            LD ++YE P  F +I+NGS LK S   + + +TT+DEA I M++LYCERA IKDG ++L
Sbjct: 77  TLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVL 136

Query: 468 DLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDME 647
           DLGCG G L L++A+KY N ++T VTN+ SQKE+I EE +K +LSNVE++LAD+T+  M 
Sbjct: 137 DLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKMP 196

Query: 648 TTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            T+DR+ V+ L EHMKN+EL LRKI +WM  D
Sbjct: 197 DTYDRILVVELFEHMKNYELLLRKIKEWMAKD 228


>ANY58196.1 putative N-methyltransferase [synthetic construct]
          Length = 357

 Score =  235 bits (600), Expect = 5e-73
 Identities = 113/218 (51%), Positives = 157/218 (72%)
 Frame = +3

Query: 90  KISQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMK 269
           KI    ++ + ++ GLIPDEE+R L+KI   +R+KWG K T  +QL   L+F KSL+ M 
Sbjct: 8   KIGSAMEILERLDKGLIPDEELRELVKIQWGRRLKWGYKSTHVQQLEFNLDFVKSLKQMD 67

Query: 270 MATELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIK 449
           M+ +++ ++S+ YE P +F + + G TLK+S   + D + T+DEA     +LYCERA IK
Sbjct: 68  MSGKMEVINSESYELPVAFLRAMFGKTLKQSCCYFEDESMTLDEAETAAHELYCERAQIK 127

Query: 450 DGQTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADV 629
           DGQ +LD+GCG G L++HIA+KY NC +TG+TN+ +QK  I EEC+ L LSNVE+ILADV
Sbjct: 128 DGQRVLDIGCGQGSLIIHIAQKYKNCHVTGITNSNAQKNIIEEECRNLKLSNVEVILADV 187

Query: 630 TSLDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
           T  D + TFDR+ +I  +EHMKN +LFL+KISKWMKDD
Sbjct: 188 TQYDTKDTFDRIILIEAVEHMKNIDLFLKKISKWMKDD 225


>ANY58193.1 putative N-methyltransferase [synthetic construct]
          Length = 360

 Score =  224 bits (571), Expect = 1e-68
 Identities = 109/216 (50%), Positives = 152/216 (70%)
 Frame = +3

Query: 96  SQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMA 275
           S+  ++   +  G I DEE+ +L++   E+R++WG KPT ++QLA   +F KSL+ M M+
Sbjct: 13  SKAGEIMGRLMKGEIGDEELSKLVRHQWERRLQWGYKPTHEQQLACNFDFIKSLKDMDMS 72

Query: 276 TELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDG 455
            E+D ++ + YE P +F +   G T+K+S   + D TTT+DEA     +LYCERA IKDG
Sbjct: 73  GEIDTMNEETYELPSAFLEATFGKTIKQSGCYFKDETTTLDEAEEASHELYCERAQIKDG 132

Query: 456 QTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTS 635
           QTILD+GCG G LVL+IA+KY NC +TG+TN+ +Q  YIL + KKL LSNV++I ADVT 
Sbjct: 133 QTILDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQMNYILMQAKKLQLSNVDVISADVTK 192

Query: 636 LDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            D + T+DR+ VI  IEHMKN +LFL+K+S WMK D
Sbjct: 193 FDNDKTYDRILVIEAIEHMKNIQLFLKKLSTWMKKD 228


>ANY58202.1 putative N-methyltransferase [synthetic construct]
          Length = 333

 Score =  222 bits (566), Expect = 3e-68
 Identities = 106/216 (49%), Positives = 152/216 (70%)
 Frame = +3

Query: 96  SQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMA 275
           S    + + +  G I DEE+ +L++   E+R++WG KPT ++QLA   +F KSL+ M M+
Sbjct: 13  SNAGKIMERLMKGEIGDEELSKLVRHMWERRLQWGYKPTHEQQLAFNFDFIKSLKHMDMS 72

Query: 276 TELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDG 455
            E+D ++ + YE P +F +   G T+K+S   + D TTT+DEA     +LYCERA IKDG
Sbjct: 73  GEIDTMNEETYELPTAFLEAAFGKTIKQSGCYFKDETTTLDEAEEASHELYCERAQIKDG 132

Query: 456 QTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTS 635
           Q++LD+GCG G LVLHIA+KY NC +TG+TN+ +Q  YIL + +KL LSNV++I ADVT 
Sbjct: 133 QSVLDIGCGQGGLVLHIAQKYKNCHVTGLTNSKAQMNYILMQAEKLQLSNVDVISADVTK 192

Query: 636 LDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            D + T+DR+ VI  IEHMKN +LF++K+S WMK+D
Sbjct: 193 FDTDKTYDRILVIEAIEHMKNIQLFMKKLSTWMKED 228


>ANY58201.1 putative N-methyltransferase [synthetic construct]
          Length = 365

 Score =  218 bits (555), Expect = 3e-66
 Identities = 100/204 (49%), Positives = 151/204 (74%)
 Frame = +3

Query: 132 GLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELDKLDSKLYE 311
           G I D+E+++L++   E+R++WG +PT  +QL+  L+F +SL+ M M+ E++ ++ + YE
Sbjct: 30  GEIGDDELKKLIRNMWERRLQWGYQPTHHQQLSFNLDFIRSLKKMDMSGEIETMNEETYE 89

Query: 312 TPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQTILDLGCGPGP 491
            P +F +   G T+K+S+  + D +TT+DEA     +LYCERA IKDGQT+LD+GCG G 
Sbjct: 90  LPTAFLEAAFGKTIKQSACYFKDKSTTLDEAEEASHELYCERAQIKDGQTVLDVGCGQGG 149

Query: 492 LVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDMETTFDRVFV 671
           LVLHIA+KY NC++TG+TN+ +Q  YI+ + +KL LSNV++ILADVT  D + T+DR+ V
Sbjct: 150 LVLHIAQKYKNCRVTGITNSKAQMNYIVMQAEKLQLSNVDVILADVTKFDSDQTYDRILV 209

Query: 672 IGLIEHMKNFELFLRKISKWMKDD 743
           I  IEHMKN +LF++K+S W+K D
Sbjct: 210 IETIEHMKNIQLFMKKLSTWLKKD 233


>ANY58195.1 TNMT [synthetic construct]
          Length = 350

 Score =  216 bits (550), Expect = 1e-65
 Identities = 102/216 (47%), Positives = 152/216 (70%)
 Frame = +3

Query: 96  SQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMA 275
           S   ++   +  G I DEE+++L++   ++RI+WG KPT ++QLA  L+F K L+ M M+
Sbjct: 3   SSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIQWGYKPTHEKQLAFNLDFIKGLKEMVMS 62

Query: 276 TELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDG 455
            E+D ++ + YE P +F + V G T+K+S   + D  +TIDEA     +LYCERA IKDG
Sbjct: 63  GEIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDG 122

Query: 456 QTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTS 635
           QT+LD+GCG G LVL+IA+KY NC +TG+TN+ +Q  YI ++ +KL L+NV++I ADVT 
Sbjct: 123 QTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELANVDVIFADVTK 182

Query: 636 LDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            D + T+DR+ V+  IEHMKN +LF++K+S WM +D
Sbjct: 183 FDTDKTYDRILVVETIEHMKNIQLFMKKLSTWMTED 218


>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
           N-methyltransferase [Eschscholzia californica]
          Length = 350

 Score =  216 bits (550), Expect = 1e-65
 Identities = 102/216 (47%), Positives = 152/216 (70%)
 Frame = +3

Query: 96  SQQDDLWKNMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMA 275
           S   ++   +  G I DEE+++L++   ++RI+WG KPT ++QLA  L+F K L+ M M+
Sbjct: 3   SSAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMS 62

Query: 276 TELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDG 455
            E+D ++ + YE P +F + V G T+K+S   + D  +TIDEA     +LYCERA IKDG
Sbjct: 63  GEIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDG 122

Query: 456 QTILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTS 635
           QT+LD+GCG G LVL+IA+KY NC +TG+TN+ +Q  YI ++ +KL L+NV++I ADVT 
Sbjct: 123 QTVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTK 182

Query: 636 LDMETTFDRVFVIGLIEHMKNFELFLRKISKWMKDD 743
            D + T+DR+ V+  IEHMKN +LF++K+S WM +D
Sbjct: 183 FDTDKTYDRILVVETIEHMKNIQLFMKKLSTWMTED 218


>XP_008220523.1 PREDICTED: (S)-coclaurine N-methyltransferase isoform X1 [Prunus
           mume]
          Length = 355

 Score =  215 bits (548), Expect = 3e-65
 Identities = 102/208 (49%), Positives = 152/208 (73%)
 Frame = +3

Query: 120 NMELGLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELDKLDS 299
           ++E  L+PD  VRRL ++ +  R++ G KPT + QL+ LL+F +SL+ M +A   D   +
Sbjct: 19  SLERNLLPDAVVRRLTRLLLASRLRSGYKPTSELQLSDLLQFVQSLKEMPIAINTDDPKA 78

Query: 300 KLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQTILDLGC 479
           + YE P SF +IV G  LK SS  +TD ++T++EA   M++LYCER+ IKDG T+LD+GC
Sbjct: 79  QHYEVPTSFFKIVLGKNLKYSSCYFTDGSSTLEEAEKAMLELYCERSQIKDGHTVLDVGC 138

Query: 480 GPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDMETTFD 659
           G G L L+IA+KYSNCK+TG+ N+ +Q+ +I E+C+ L L NVEII+AD+++ +ME ++D
Sbjct: 139 GWGSLPLYIAQKYSNCKVTGICNSTTQQAFIEEQCRNLQLQNVEIIVADISTFEMEASYD 198

Query: 660 RVFVIGLIEHMKNFELFLRKISKWMKDD 743
           R+F I + EHMKN++  L+KIS+WMK D
Sbjct: 199 RIFSIEMFEHMKNYKYLLKKISRWMKQD 226


>ANY58198.1 putative N-methyltransferase [synthetic construct]
          Length = 359

 Score =  215 bits (548), Expect = 3e-65
 Identities = 100/204 (49%), Positives = 149/204 (73%)
 Frame = +3

Query: 132 GLIPDEEVRRLMKIGIEKRIKWGTKPTQQEQLAQLLEFNKSLRGMKMATELDKLDSKLYE 311
           G I DEE+++L+K  +EKR++WG K + QEQL+  L+F KSL+ M+M+ E++ ++ + YE
Sbjct: 22  GEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEMSGEIETMNKETYE 81

Query: 312 TPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQTILDLGCGPGP 491
            P  F + V G T+K+S   +   ++TIDEA     +LYCERA IKDGQT+LD+GCG G 
Sbjct: 82  LPSEFLEAVFGKTVKQSMCYFKHESSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGG 141

Query: 492 LVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSLDMETTFDRVFV 671
           LVL+IA+KY  C +TG+TN+  Q  YIL++ +KL L NV+++LADVT  + + T+DR+ V
Sbjct: 142 LVLYIAQKYKKCHVTGLTNSKEQVNYILKQAEKLGLRNVDVMLADVTQYESDKTYDRILV 201

Query: 672 IGLIEHMKNFELFLRKISKWMKDD 743
           IG +EH+KN +LF++K+S WM +D
Sbjct: 202 IGFVEHIKNLQLFMKKLSTWMAED 225


>ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata]
          Length = 357

 Score =  215 bits (547), Expect = 4e-65
 Identities = 106/212 (50%), Positives = 149/212 (70%), Gaps = 3/212 (1%)
 Frame = +3

Query: 108 DLWKNMELGLIPDEEVRRLMKIGIEKRIKW--GTKPTQQEQLAQLLEFNKSLRGMKMA-T 278
           ++ + +E G +PDEE RRL++I + +R++W    KPT +EQ A+++   K+LR M +   
Sbjct: 10  EMLRRLEEGSVPDEEFRRLIRIELGRRLRWYCQKKPTYEEQTAEIVALVKALRQMGITGD 69

Query: 279 ELDKLDSKLYETPHSFNQIVNGSTLKESSGLYTDVTTTIDEASIKMMDLYCERANIKDGQ 458
           + D+L+S LY+ P SF +I  G  LKES   + D + T+DEA   M+DLYCERA +KDGQ
Sbjct: 70  QSDQLNSDLYDMPMSFLKITFGKLLKESGSYFKDDSMTLDEAEEAMLDLYCERAQLKDGQ 129

Query: 459 TILDLGCGPGPLVLHIAKKYSNCKITGVTNAFSQKEYILEECKKLSLSNVEIILADVTSL 638
            ILDLGCG G   LH A+KY    +T VTN+ +QK+YI ++C+ L LSNVE++L D+T L
Sbjct: 130 KILDLGCGQGAFTLHAAQKYKKSHVTAVTNSATQKKYIEDQCQILELSNVEVLLEDITQL 189

Query: 639 DMETTFDRVFVIGLIEHMKNFELFLRKISKWM 734
            ME TFDR+ VIGL+EHMKN+ L L+ IS+WM
Sbjct: 190 TMEATFDRIIVIGLLEHMKNYGLLLQNISQWM 221