BLASTX nr result
ID: Papaver32_contig00033787
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00033787
(1261 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 586 0.0
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 584 0.0
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 581 0.0
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik... 570 0.0
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 541 0.0
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 539 0.0
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 538 0.0
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 499 e-172
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 495 e-170
AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum fla... 481 e-165
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 348 e-113
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 340 e-109
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 339 e-109
XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 335 e-107
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 331 e-105
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 328 e-105
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 325 e-104
XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 325 e-103
XP_002274586.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 324 e-103
CAN70170.1 hypothetical protein VITISV_006874 [Vitis vinifera] 324 e-102
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 586 bits (1511), Expect = 0.0
Identities = 284/408 (69%), Positives = 345/408 (84%), Gaps = 1/408 (0%)
Frame = -3
Query: 1223 ALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKTYGDLFTI 1044
+L+A+V T F +LI RDSS PIVGNLLQL +KPHS FA+LAKTYGDLFT+
Sbjct: 6 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 65
Query: 1043 KLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNKLRR 864
KLG+ET+VVAST +AA+EILKTHDR+LSGRYVFQSFRVK V+ S+ WS+CN+TW KLR+
Sbjct: 66 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 125
Query: 863 ICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQN 684
+C+ ELF+QKMIESQAEIRES A+EMVE+LK+ +GSEVK+ +V+FGT++N+FG IFSQN
Sbjct: 126 VCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQN 185
Query: 683 IFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRVYDVW 504
IF+LGDE+SGS+ MKE + R++ELGN+ NPADYFPFLG +DL GQRK+VA+CLQ +Y VW
Sbjct: 186 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVW 245
Query: 503 GVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXEWAVA 327
G +LKERKI + HN+ KKNDFV++LLD+GL+D QIN+L+M EWA++
Sbjct: 246 GAMLKERKIAKL-HNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 304
Query: 326 ELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPRRALE 147
EL KNP+V A MRSELLSVVG+RP+KE+DIPNMPYLQAF KETLRLHP TPLL+PRRALE
Sbjct: 305 ELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 364
Query: 146 TCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
TCK+LNYTIPK+CQIMVNAWGIGRDP+ WTDPLKF+PERFLNSSIDFK
Sbjct: 365 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFK 412
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 584 bits (1506), Expect = 0.0
Identities = 284/408 (69%), Positives = 343/408 (84%), Gaps = 1/408 (0%)
Frame = -3
Query: 1223 ALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKTYGDLFTI 1044
+L+A+V T F +LI RDSS PIVGNLLQL +KPHS FAQLA+TYGDLF++
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 1043 KLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNKLRR 864
KLG+ET+VVAST +AA+EILKTHDR+LSGRYVFQSFRVK V+ S+ WS+CN+TW KLR+
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 863 ICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQN 684
+C+ ELF+QKMIESQAE+RES A+EMVEYLKK G+EVK+++V+FGT++N+FG IFSQN
Sbjct: 121 VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 683 IFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRVYDVW 504
IF+LGDE+SGS+ MKE + R++ELGN+ NPADYFPFLG +DL GQ K+VA+CLQ +Y VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 503 GVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXEWAVA 327
G +LKERKI QHN+ KKNDFV++LLD+GL+D QIN+L+M EWA++
Sbjct: 241 GAMLKERKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299
Query: 326 ELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPRRALE 147
EL KNPQV A MR ELLSVVG+RP+KE+DIPNMPYLQAF KETLRLHP TPLL+PRRALE
Sbjct: 300 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359
Query: 146 TCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
TCK+LNYTIPK+CQIMVNAWGIGRDP+ WTDPLKFSPERFLNSSIDFK
Sbjct: 360 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFK 407
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 581 bits (1497), Expect = 0.0
Identities = 282/408 (69%), Positives = 342/408 (83%), Gaps = 1/408 (0%)
Frame = -3
Query: 1223 ALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKTYGDLFTI 1044
+L+A+V T F +LI RDSS PIVGNLLQL +KPHS FAQLA+TYGDLF++
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 1043 KLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNKLRR 864
KLG+ET+VVAST +AA+EILKTHDR+LSGRYVFQSFRVK V+ S+ WS+CN+TW KLR+
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 863 ICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQN 684
+C+ +LF+QKMIESQAE+RES A+EMVEYLKK G+EVK+++V+FGT++N+FG IFSQN
Sbjct: 121 VCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 683 IFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRVYDVW 504
IF+LGDE+SGS+ MKE + R++ELGN+ NPADYFPFLG +DL GQ K+VA+CLQ +Y VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 503 GVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXEWAVA 327
G +LKE KI QHN+ KKNDFV++LLD+GL+D QIN+L+M EWA++
Sbjct: 241 GAMLKESKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299
Query: 326 ELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPRRALE 147
EL KNPQV A MR ELLSVVG+RP+KE+DIPNMPYLQAF KETLRLHP TPLL+PRRALE
Sbjct: 300 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359
Query: 146 TCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
TCK+LNYTIPK+CQIMVNAWGIGRDP+ WTDPLKFSPERFLNSSIDFK
Sbjct: 360 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFK 407
>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
P450 [Papaver somniferum]
Length = 440
Score = 570 bits (1470), Expect = 0.0
Identities = 284/415 (68%), Positives = 340/415 (81%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
MEIVT+A LIAIVFT F +LI+R+SS PIVGNLLQL +KPHS FAQLA+T
Sbjct: 1 MEIVTVA-LIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAET 59
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YGDLFT+KLGT+T+VVAST +AA+E+LK HDR L GRYVFQSFRVK V+ S+ W++CN+
Sbjct: 60 YGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNE 119
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
TW KLR++C+ +LF+QKMIE+QAE+RE +EMV+YLKK EG EVK+ +VIFGT++NMFG
Sbjct: 120 TWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFG 179
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFSQ+IF+LGDE+SGSL MK+ I R++ELGN+ NP DY P LGS DL GQRK+VA+CL
Sbjct: 180 NLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCL 239
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
Q VY VWG +LKER+I Q N KNDFVDVLLD+GL+D QINSL++
Sbjct: 240 QGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAS 299
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL KNPQV A +RSELLSVVG+R +KE+DI N+PYLQAF KETLRLHPPTPLL
Sbjct: 300 TIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLL 359
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
IPRRALETC++LNYTIPK+CQIMVNAWGIGRDP+TWTDPLKFSP+RFLNSSIDFK
Sbjct: 360 IPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFK 414
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 541 bits (1393), Expect = 0.0
Identities = 265/415 (63%), Positives = 324/415 (78%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
ME+VT+A LIA++ + +L+ S PIVGNLLQL +KPH+ FA+LA+T
Sbjct: 1 MEVVTVA-LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQT 59
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YGD+FT+K+GTET+VVASTS AA+EILKTHDRILS RYVFQSFRVKG V+ S+ WSDC +
Sbjct: 60 YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTE 119
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
TW LR++C+ ELF+QKMIESQA +RE EMVEYL KK+G EVK+ +VIFGT++N+FG
Sbjct: 120 TWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFG 179
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFSQNIFELGD NSGS KE + R++ELGN+ NPADYFP LG +DL GQRKEVA CL
Sbjct: 180 NLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECL 239
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
+ +Y +WG +L+ERK+ + KNDFVDV LD+GL D QIN+L+M
Sbjct: 240 KGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAS 299
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL KNP++ AK+RSEL +VVG+R +KE+D PN+PYL+A KETLRLHPPTPLL
Sbjct: 300 TIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLL 359
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
+PRRALETC ILNYTIPKDCQIMVNAWGIGRDP+TW DPL FSPERFLNSS+DF+
Sbjct: 360 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFR 414
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 539 bits (1388), Expect = 0.0
Identities = 262/408 (64%), Positives = 319/408 (78%), Gaps = 1/408 (0%)
Frame = -3
Query: 1223 ALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKTYGDLFTI 1044
ALIA++ + +L+ S PIVGNLLQL +KPH+ FA+LA+TYGD+FT+
Sbjct: 6 ALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 65
Query: 1043 KLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNKLRR 864
K+GTET+VVASTS AA+EILKTHDRILS RYVFQSFRVKG V+ S+ WSDC +TW LR+
Sbjct: 66 KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRK 125
Query: 863 ICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQN 684
+C+ ELF+QKMIESQA +RE EMVEYL KK+G EVK+ +VIFGT++N+FG IFSQN
Sbjct: 126 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 185
Query: 683 IFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRVYDVW 504
IFELGD NSGS KE + R++ELGN+ NPADYFP LG +DL GQRKEVA CL+ +Y +W
Sbjct: 186 IFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 245
Query: 503 GVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXEWAVA 327
G +L+ERK+ K KNDFVDV LD+GL D QIN+L+M EWA+
Sbjct: 246 GAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 305
Query: 326 ELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPRRALE 147
EL KNP++ AK+RSE+ +VVG+R +KE+D PN+PYL+A KETLRLHPPTPLL+PRRALE
Sbjct: 306 ELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALE 365
Query: 146 TCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
TC ILNYTIPKDCQIMVNAWGIGRDP+TWTDPL FSPERFLNSS+DF+
Sbjct: 366 TCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFR 413
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 538 bits (1386), Expect = 0.0
Identities = 264/415 (63%), Positives = 323/415 (77%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
ME+VT+A LIA++ + +L+ S PIVGNLLQL +KPH+ FA+LA+T
Sbjct: 1 MEVVTVA-LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQT 59
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YGD+FT+K+GTET+VVASTS AA+EILKTHDRILS RYVFQSFRVKG V+ S+ WSDC +
Sbjct: 60 YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTE 119
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
TW LR++C+ ELF+QKMIESQA +RE EMVEYL KK+G EVK+ +VIFGT++N+FG
Sbjct: 120 TWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFG 179
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFSQNIFELG NSGS KE + R++ELGN+ NPADYFP LG +DL GQRKEVA CL
Sbjct: 180 NLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECL 239
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
+ +Y +WG +L+ERK+ + KNDFVDV LD+GL D QIN+L+M
Sbjct: 240 KGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAS 299
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL KNP++ AK+RSEL +VVG+R +KE+D PN+PYL+A KETLRLHPPTPLL
Sbjct: 300 TIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLL 359
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
+PRRALETC ILNYTIPKDCQIMVNAWGIGRDP+TW DPL FSPERFLNSS+DF+
Sbjct: 360 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFR 414
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 499 bits (1284), Expect = e-172
Identities = 241/415 (58%), Positives = 320/415 (77%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
ME++++A +++ F +F F ILRDS PIVGNLLQL DKPH+ FA+LA+
Sbjct: 1 MEVLSIA-IVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQK 59
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YG+LF++KLG++T+VVAS+ AA EILKTHD+ILSGRYVFQSFRVK V+ S+ WS+CND
Sbjct: 60 YGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECND 119
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
W LR++C+ ELF+ KMIESQ+EIRE+ A EMV++L+ KEG VK+ +V+FGT++N+FG
Sbjct: 120 NWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFG 179
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFS+++F+L D GS+ +KE + +L+++GN+ NPADYFP +G DL GQR+ VA L
Sbjct: 180 NLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVL 239
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
Q++YDVWGV+LKER+ S+ KNDFVDVLL+AGL+D +IN+L+M
Sbjct: 240 QQIYDVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAS 296
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL K P V +K+R EL++VVG +KE+D+P++PYLQAF KETLRLHPPTPLL
Sbjct: 297 TIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLL 356
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
+PRRALETC ++NYTIPK+CQIMVNAW IGRDP+TW DPL F PERFL+S +D+K
Sbjct: 357 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYK 411
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 495 bits (1274), Expect = e-170
Identities = 239/415 (57%), Positives = 316/415 (76%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
ME+V++A +++ F +F F ILRD PIVGNLLQL DKP + FA+LA+
Sbjct: 1 MEVVSIA-IVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQK 59
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YG LF++KLG++T+VVAS+ AA E+LKTHD+ILSGRYVFQSFRVK V+ S+ WS+CND
Sbjct: 60 YGQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECND 119
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
W LR++C+ ELF+ KMIESQ+EIRE A EMV++L+ KEG VK+ +V+FGT++N+FG
Sbjct: 120 NWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFG 179
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFS+++F+L D GS+ +KE + +L+E+GN+ NPADYFP +G +DL GQR+ VA+ L
Sbjct: 180 NLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVL 239
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
Q++YDVWG+ LKER+ S+ NDFVDVLL+AGLED +IN+L+M
Sbjct: 240 QQIYDVWGINLKERR---GMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSAS 296
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL K P V +K+R EL++VVG +KE+D+P++PYLQAF KETLRLHPPTPLL
Sbjct: 297 TIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLL 356
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
+PRRALETC ++NYTIPK+CQIMVNAW IGRDP+TW DPL F PERFL+S +D+K
Sbjct: 357 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYK 411
>AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 481 bits (1239), Expect = e-165
Identities = 232/415 (55%), Positives = 313/415 (75%), Gaps = 1/415 (0%)
Frame = -3
Query: 1244 MEIVTLAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSDKPHSHFAQLAKT 1065
ME+++ A + +F F IL DS PIVGNLLQL +KPH+ FA+LA+
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 1064 YGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCND 885
YG+LFT+KLG++T+VVAS+ AA EILKT D+ILSGRYVFQSFRV V S+ WS+CN+
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 884 TWNKLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFG 705
W LR++C+ ELFS KMIESQA IRE+ AL+MV +L+KKE EVK+ +V+F T++N+FG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 704 KSIFSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCL 525
IFS+++F+L D GS +KE + +L+++GN+ NPADYFP +G +DL GQR+EVA L
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 524 QRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXX 348
+++YDVWGV+LKER+ ++NDF +VLL+AGL+D +IN+L+M
Sbjct: 241 KQIYDVWGVMLKERRSI----TGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTAS 296
Query: 347 XXEWAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLL 168
EWA+ EL N +V +K+R+EL++VVG + +KE++IP++PYLQA KETLRLHPPTPLL
Sbjct: 297 TIEWAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLL 356
Query: 167 IPRRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
+PRRALETCK++NYTIPK+CQIMVNAW IGRDP+TW DPL F PERF+NS++D+K
Sbjct: 357 LPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYK 411
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 348 bits (893), Expect = e-113
Identities = 175/412 (42%), Positives = 262/412 (63%), Gaps = 3/412 (0%)
Frame = -3
Query: 1229 LAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLL-QLSDKPHSHFAQLAKTYGDL 1053
L + I + +I ++ S PI+G LL +L +PH ++LA+ +G L
Sbjct: 4 LVSFIFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRFGPL 63
Query: 1052 FTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNK 873
+K G E +VVAS+ +AA E+LK DR+LSGR+ S R+KG ++ SM W+DC D W
Sbjct: 64 MLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKM 123
Query: 872 LRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIF 693
+R++ + ELFS KM++ QA RE E++++L +KEG +V +DVIFG+++N+ G IF
Sbjct: 124 VRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIF 183
Query: 692 SQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRVY 513
S+++++ D +L MK IR+LM L N AD +P LG D G RK A C++R+
Sbjct: 184 SKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMN 243
Query: 512 DVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXEW 336
+ W ++K+R+ +++ KNDF+ VLLD+G D QI+++++ EW
Sbjct: 244 ESWAAIVKQRR----KNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEW 299
Query: 335 AVAELAKNPQVAAKMRSELLSVVGQ-RPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPR 159
A+AEL +NP+ K+R EL V+ + +KE+D+PN+PYL A KETLRLHPP L+P
Sbjct: 300 AMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPH 359
Query: 158 RALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
RA+ETC+++NYTIPK CQ+MVN + IGRD +TW PL F PERFLNS +D++
Sbjct: 360 RAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLNSELDYQ 411
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 340 bits (871), Expect = e-109
Identities = 171/380 (45%), Positives = 236/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GNLLQ+ PH+ A LAK +G L +++LGT+ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 950 VFQSFRVKGQV--KLSMTWS-DCNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
+ S VK LS+ ++ DCN W LR IC++ELFS K +ESQ E+RE E+VE
Sbjct: 107 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L KEG VK+ D++F T+ N+ FS ++ + DE +K+ I + E G T
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N +DY+P LG D+ G ++ +R+ W +LKER+ + N DF++ LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEALLE 284
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
G ED QIN +I+ WA+AEL +N K+R EL +VG+ P++E+
Sbjct: 285 IGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRES 344
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+P +PYLQA KE LRLHPP PLL+P A ETC+++ YTIPKD QI VN W + RDP+
Sbjct: 345 HLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKI 404
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DPL F PERFL+S +DFK
Sbjct: 405 WDDPLSFKPERFLDSKLDFK 424
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 339 bits (870), Expect = e-109
Identities = 171/380 (45%), Positives = 236/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GNLLQ+ PH+ A LAK +G L +++LGT+ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 950 VFQSFRVKGQV--KLSMTWS-DCNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
+ S VK LS+ ++ DCN W LR IC++ELFS K +ESQ E+RE E+VE
Sbjct: 107 LSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L KEG VK+ D++F T+ N+ FS ++ + DE +K+ I + E G T
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N +DY+P LG D+ G ++ +R+ W +LKER+ + N DF++ LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEALLE 284
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
G ED QIN +I+ WA+AEL +N K+R EL +VG+ P++E+
Sbjct: 285 IGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRES 344
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+P +PYLQA KE LRLHPP PLL+P A ETC+++ YTIPKD QI VN W + RDP+
Sbjct: 345 HLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKI 404
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DPL F PERFL+S +DFK
Sbjct: 405 WDDPLSFKPERFLDSKLDFK 424
>XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 335 bits (859), Expect = e-107
Identities = 174/413 (42%), Positives = 253/413 (61%), Gaps = 4/413 (0%)
Frame = -3
Query: 1229 LAALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQLSD-KPHSHFAQLAKTYGDL 1053
LA I + +I ++ S PI+G L KP+ +LA+ YG L
Sbjct: 4 LALFILFLLSILSLVLFLKPSSKKLPPGPFSWPIIGTQLPSPTMKPNLELFKLAQRYGPL 63
Query: 1052 FTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWNK 873
+ G E +VVAS +AA E+LK DR+LSGR+ S RVKG ++ SM W+DC D W
Sbjct: 64 MLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKM 123
Query: 872 LRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSIF 693
+R+I + ELFS KM++ A +RE E++++L++KEG EV DVIFG ++NM G I+
Sbjct: 124 VRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIY 183
Query: 692 SQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFP-FLGSWDLLGQRKEVANCLQRV 516
S+++++ D+ +L MK IR+LM L T N AD +P F D G RKE A C++R+
Sbjct: 184 SKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACVKRM 243
Query: 515 YDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXE 339
+ W ++ ER+ ++N KND + VLLD+G D QI+++ + E
Sbjct: 244 SESWAAIINERR----KNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIE 299
Query: 338 WAVAELAKNPQVAAKMRSELLSVVGQ-RPIKEADIPNMPYLQAFSKETLRLHPPTPLLIP 162
WA+AEL +NP+ K+ EL V+G+ +K++D+PN+PYL A KETLRLHPP P LIP
Sbjct: 300 WALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVKETLRLHPPVPFLIP 359
Query: 161 RRALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDFK 3
ALE+C+++NYTIPK +++VN + IGRDP TW +P F PERFLNS +D++
Sbjct: 360 HIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTWDNPNSFLPERFLNSEVDYQ 412
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 331 bits (848), Expect = e-105
Identities = 166/380 (43%), Positives = 238/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GNLLQL PH A LAK +G L +++LGT+ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRY 106
Query: 950 VFQSFRVKGQV--KLSMTWS-DCNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
V S VK LS+ ++ +C + W LR IC+ E+FS K +ES E+RE +E+VE
Sbjct: 107 VSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVE 166
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L KEG VK+ D++F T+ N+ + FS ++ + E +K+ I R ELG T
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATP 221
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N +D++P L +L G +K+ L R+ W LKER+ ++ D ++ L+
Sbjct: 222 NLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLE 279
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
ED QIN +I+ WA+ +L +NP V K+R EL ++G+ P++E+
Sbjct: 280 IRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRES 339
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+P++PYLQA KETLRLHPP PLL+P RA+ETC+++ YTIPKD Q+ VN W +GRDP+
Sbjct: 340 HLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKV 399
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DPL F+PERFL+S ++FK
Sbjct: 400 WDDPLSFTPERFLDSKLEFK 419
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 328 bits (842), Expect = e-105
Identities = 172/411 (41%), Positives = 254/411 (61%), Gaps = 5/411 (1%)
Frame = -3
Query: 1223 ALIAIVFTIFFFLILRDSSXXXXXXXXXXXPIVGNLLQL---SDKP-HSHFAQLAKTYGD 1056
AL +++ I F++L P++GNL L ++ P H A +A+T+G
Sbjct: 6 ALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGP 65
Query: 1055 LFTIKLGTETIVVASTSIAATEILKTHDRILSGRYVFQSFRVKGQVKLSMTWSDCNDTWN 876
+ + LGT+ V+AST+ AA EILKTHDRI S R++ SFR+K +K S+ WSDC D W
Sbjct: 66 IMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWK 125
Query: 875 KLRRICKVELFSQKMIESQAEIRESTALEMVEYLKKKEGSEVKMSDVIFGTVMNMFGKSI 696
LR+I + E+FS KM+++Q+ +RE E++++L+ KEG VK+S +FGT++N+ G +
Sbjct: 126 LLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVV 185
Query: 695 FSQNIFELGDENSGSLNMKERIRRLMELGNTKNPADYFPFLGSWDLLGQRKEVANCLQRV 516
FS+++F GDE ++ IR ++ +G N A+++P L DL G +K+ RV
Sbjct: 186 FSKDVFVYGDETDKG-GIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRV 244
Query: 515 YDVWGVVLKERKIFNMQHNSKKKNDFVDVLLDAGLEDGQINSLIM-XXXXXXXXXXXXXE 339
+W +KERK + +++ D +DVLL D QIN+L + E
Sbjct: 245 MKMWEGTVKERK----ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 300
Query: 338 WAVAELAKNPQVAAKMRSELLSVVGQRPIKEADIPNMPYLQAFSKETLRLHPPTPLLIPR 159
W +AEL K+P+ AK+R EL VVG IKE+D+P +PYLQA KE +RLHP P L+PR
Sbjct: 301 WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPR 360
Query: 158 RALETCKILNYTIPKDCQIMVNAWGIGRDPETWTDPLKFSPERFLNSSIDF 6
RA ETC+++ YTIPK+ Q++VNA+ IGRDP++W DP F PERFL S +DF
Sbjct: 361 RAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDF 411
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 325 bits (834), Expect = e-104
Identities = 170/381 (44%), Positives = 241/381 (63%), Gaps = 5/381 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GN+LQ+ PH A AK +G L +++LGT +VVAS+ AA EILKTHDR+LSGRY
Sbjct: 46 ILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRY 105
Query: 950 VFQSFRVKGQV--KLSMTWS-DCNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
+ + + V ++++ W+ C+D W LR +C+ ELFS K IESQA +RE EM++
Sbjct: 106 IPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLD 165
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDE-NSGSLNMKERIRRLMELGNT 603
+L K+G V + +V+F T N +FS+++ D+ N+G L K I LMEL
Sbjct: 166 FLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAGEL--KTLISTLMELATC 223
Query: 602 KNPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLL 423
N AD++P L D G ++++ NCL+R++ VW + +KER+ +++ +K DF+DV L
Sbjct: 224 PNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVK--DARKTDFLDVFL 281
Query: 422 DAGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKE 246
G +D QIN L++ WA+AEL K V K+R EL + +G+ I+E
Sbjct: 282 SNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRE 341
Query: 245 ADIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPE 66
+ IP + YL A KETLRLHPP P LIPRRA E C+++NYTIPK QI+VN W IGRDP
Sbjct: 342 SHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPS 401
Query: 65 TWTDPLKFSPERFLNSSIDFK 3
W DPL + PERFL+S++DFK
Sbjct: 402 AWEDPLSYRPERFLDSNLDFK 422
>XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus
communis] EEF51432.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 325 bits (833), Expect = e-103
Identities = 159/380 (41%), Positives = 236/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GN+LQ+ K H A AK YG L +++LG + +VVAST +A E+LK HDR+LS R+
Sbjct: 45 ILGNILQVDKKLHICIANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARF 104
Query: 950 VFQSFRVKGQV--KLSMTWSD-CNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
+ ++ + + ++++ W+ CND W LR +C+ ELFS K IESQA +RE EM+E
Sbjct: 105 ILKAIPSESHILERVAIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLE 164
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L K+G V +++V+FGT+ N +FS ++ ++ G +K + +ME+ +
Sbjct: 165 FLITKQGQAVNVAEVVFGTIFNTISHLLFSTDLIGFENQIGG---VKSLLWSMMEMATSP 221
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N A+++P L D G ++++ CL+ ++ VW + +KER+ + ++ K DF+D+ L
Sbjct: 222 NIAEFYPILAPLDPQGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLS 281
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
G +D QIN L+M WA+AEL KN + K+R EL + + PIKE+
Sbjct: 282 NGFDDDQINWLVMELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKES 341
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+ +PYL A KETLRLHPP P LIPRRA E C+++NYTIPKD Q++VN W IGRDP
Sbjct: 342 HVSQLPYLNACVKETLRLHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSV 401
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DP F PERFL SS+D K
Sbjct: 402 WEDPSSFKPERFLGSSLDVK 421
>XP_002274586.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 324 bits (831), Expect = e-103
Identities = 169/380 (44%), Positives = 238/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GNLLQ+ H A LAK +G L +++LGT+ +VVAS+S AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRY 106
Query: 950 VFQSFRVKGQV--KLSMTWSD-CNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
V + V L+M ++ CN W L+ IC++ELFS K +ES+ E+RE +E+VE
Sbjct: 107 VSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVE 166
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L+KKEG VK+ D+++ TV N+ FS + + + + +K+ + ELG T
Sbjct: 167 FLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELGAT- 225
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N D++P LG D+ G RK++ +R+ W +LKER+ + + DF+D LL+
Sbjct: 226 NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDFLDALLE 283
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
G ED QIN +IM WA+AEL KN K+ +EL ++G+ P++E+
Sbjct: 284 TGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRES 343
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+P++PYLQA KETLRLHP PLL+P RA ETC+I+ YTIPKD I VN W +GRDP T
Sbjct: 344 HLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGT 403
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DPL F PERFL+S ++FK
Sbjct: 404 WEDPLSFKPERFLDSKLEFK 423
>CAN70170.1 hypothetical protein VITISV_006874 [Vitis vinifera]
Length = 559
Score = 324 bits (831), Expect = e-102
Identities = 169/380 (44%), Positives = 238/380 (62%), Gaps = 4/380 (1%)
Frame = -3
Query: 1130 IVGNLLQLSDKPHSHFAQLAKTYGDLFTIKLGTETIVVASTSIAATEILKTHDRILSGRY 951
I+GNLLQ+ H A LAK +G L +++LGT+ +VVAS+S AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRY 106
Query: 950 VFQSFRVKGQV--KLSMTWSD-CNDTWNKLRRICKVELFSQKMIESQAEIRESTALEMVE 780
V + V L+M ++ CN W L+ IC++ELFS K +ES+ E+RE +E+VE
Sbjct: 107 VSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVE 166
Query: 779 YLKKKEGSEVKMSDVIFGTVMNMFGKSIFSQNIFELGDENSGSLNMKERIRRLMELGNTK 600
+L+KKEG VK+ D+++ TV N+ FS + + + + +K+ + ELG T
Sbjct: 167 FLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELGAT- 225
Query: 599 NPADYFPFLGSWDLLGQRKEVANCLQRVYDVWGVVLKERKIFNMQHNSKKKNDFVDVLLD 420
N D++P LG D+ G RK++ +R+ W +LKER+ + + DF+D LL+
Sbjct: 226 NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDFLDALLE 283
Query: 419 AGLEDGQINSLIMXXXXXXXXXXXXXE-WAVAELAKNPQVAAKMRSELLSVVGQRPIKEA 243
G ED QIN +IM WA+AEL KN K+ +EL ++G+ P++E+
Sbjct: 284 TGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRES 343
Query: 242 DIPNMPYLQAFSKETLRLHPPTPLLIPRRALETCKILNYTIPKDCQIMVNAWGIGRDPET 63
+P++PYLQA KETLRLHP PLL+P RA ETC+I+ YTIPKD I VN W +GRDP T
Sbjct: 344 HLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGT 403
Query: 62 WTDPLKFSPERFLNSSIDFK 3
W DPL F PERFL+S ++FK
Sbjct: 404 WEDPLSFKPERFLDSKLEFK 423