BLASTX nr result
ID: Papaver32_contig00025353
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00025353
(588 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_015383539.1 PREDICTED: uncharacterized protein LOC107176045 [... 78 1e-13
XP_010474601.1 PREDICTED: uncharacterized protein LOC104754160 [... 65 5e-09
XP_017251150.1 PREDICTED: uncharacterized protein LOC108221805 [... 63 1e-08
XP_013608022.1 PREDICTED: uncharacterized protein LOC106314737 [... 62 2e-08
XP_013624244.1 PREDICTED: uncharacterized protein LOC106330293 [... 62 3e-08
XP_012088409.1 PREDICTED: uncharacterized protein LOC105647037 [... 60 6e-08
XP_015389111.1 PREDICTED: uncharacterized protein LOC107178429 [... 60 8e-08
XP_015387924.1 PREDICTED: uncharacterized protein LOC107177897 [... 60 1e-07
XP_015387369.1 PREDICTED: uncharacterized protein LOC107177677 [... 58 1e-07
XP_013639539.1 PREDICTED: uncharacterized protein LOC106344775 [... 61 1e-07
XP_013625423.1 PREDICTED: uncharacterized protein LOC106331580 [... 60 2e-07
XP_015388029.1 PREDICTED: uncharacterized protein LOC107177961 [... 60 2e-07
XP_009113118.1 PREDICTED: uncharacterized protein LOC103838431 [... 60 2e-07
XP_013601608.1 PREDICTED: uncharacterized protein LOC106309082 [... 60 2e-07
XP_004298055.1 PREDICTED: uncharacterized protein LOC101313796 [... 58 3e-07
XP_013594814.1 PREDICTED: uncharacterized protein LOC106302971 [... 60 3e-07
ONI01475.1 hypothetical protein PRUPE_6G141500 [Prunus persica] 60 3e-07
XP_007206484.1 hypothetical protein PRUPE_ppa019056mg, partial [... 60 3e-07
XP_009368150.1 PREDICTED: uncharacterized protein LOC103957683 [... 59 4e-07
XP_010490159.1 PREDICTED: uncharacterized protein LOC104767890 [... 60 4e-07
>XP_015383539.1 PREDICTED: uncharacterized protein LOC107176045 [Citrus sinensis]
Length = 381
Score = 78.2 bits (191), Expect = 1e-13
Identities = 39/109 (35%), Positives = 65/109 (59%), Gaps = 5/109 (4%)
Frame = +2
Query: 77 TTSRAEQFPLIASMLWSIWESRNEI-----VQEPITILSRAKAMMLNSTKNPFINRTSAI 241
+ S+AE F L+A+ +W+IW +RNE + +P ++L++A+AM + + F T+A
Sbjct: 156 SVSKAE-FELLAASMWAIWHARNEFHFNKSISDPSSVLAKAEAMCNSYQRISFQEETAAR 214
Query: 242 TQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
++++S T W PP NW K+N D A N E GLG ++R+ E + A
Sbjct: 215 SRSESTVTGWKPPPENWFKLNVDAATNAGKERAGLGAIVRNWKGEIMAA 263
>XP_010474601.1 PREDICTED: uncharacterized protein LOC104754160 [Camelina sativa]
Length = 1368
Score = 65.5 bits (158), Expect = 5e-09
Identities = 39/146 (26%), Positives = 77/146 (52%), Gaps = 13/146 (8%)
Frame = +2
Query: 20 WLTFIQWIRYWMQLYPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPITILSRA 184
W I +W+ Y +++ S+ L +LW IW++RN ++ E I+++++A
Sbjct: 1121 WSNSIYANLFWVFNY-HKELPSQENLSSLSPWILWRIWKNRNNLIFKGREYEAISLINKA 1179
Query: 185 KA--------MMLNSTKNPFINRTSAITQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIG 340
K + L K+P N ++ +N+ W+ P ++W+K N+DGAW + + G
Sbjct: 1180 KEDAEEWSQRLELRGIKHP--NPPGSLNRNK-----WIKPSQDWVKCNSDGAWPHDGDTG 1232
Query: 341 GLGFLIRDSSNEFIYAEAKNLVCGRT 418
GLG+++RD+ + ++A + + RT
Sbjct: 1233 GLGWVLRDNRGKILWAGVRLVSKTRT 1258
>XP_017251150.1 PREDICTED: uncharacterized protein LOC108221805 [Daucus carota
subsp. sativus]
Length = 231
Score = 62.8 bits (151), Expect = 1e-08
Identities = 36/102 (35%), Positives = 54/102 (52%), Gaps = 5/102 (4%)
Frame = +2
Query: 122 WSIWESRNEIVQEPITILS-RAKAMMLNS----TKNPFINRTSAITQNQSIPTPWVVPPR 286
W++W RN+ V E +T+ S K+M LN +K + Q + T W PP
Sbjct: 7 WALWYRRNKWVWERVTVSSFGVKSMALNMKTDWSKAREVEEHHEGQQQMRVHT-WCKPPE 65
Query: 287 NWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEAKNLVCG 412
W+KINTD A + N E+ GLG + RD S +F+ A ++ + G
Sbjct: 66 QWIKINTDAACHVNGEMTGLGCVARDESGQFVRARSRKVQVG 107
>XP_013608022.1 PREDICTED: uncharacterized protein LOC106314737 [Brassica oleracea
var. oleracea]
Length = 260
Score = 62.4 bits (150), Expect = 2e-08
Identities = 37/123 (30%), Positives = 63/123 (51%), Gaps = 11/123 (8%)
Frame = +2
Query: 62 YPYQDTTSRAEQFPLIASMLWSIWESRNEIVQ-----EPITILSRAKAMMLN-----STK 211
YP +D Q ++ +LW +W++RNE++ E +++ +A+ + K
Sbjct: 18 YPREDI-----QAEIVPWILWRLWKNRNELLFKGKEFEAGSLILKAREDLEEWQGSCQAK 72
Query: 212 NPFINRTSAITQNQSIPT-PWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
+ A S+ T W PP +WLK N+DGAW+ N E GLG++ RD+ ++A
Sbjct: 73 ITAAEKIEAKRATTSVQTHTWKPPPTDWLKCNSDGAWHQNRETSGLGWICRDAIGHVLWA 132
Query: 389 EAK 397
EA+
Sbjct: 133 EAR 135
>XP_013624244.1 PREDICTED: uncharacterized protein LOC106330293 [Brassica oleracea
var. oleracea]
Length = 260
Score = 62.0 bits (149), Expect = 3e-08
Identities = 37/123 (30%), Positives = 63/123 (51%), Gaps = 11/123 (8%)
Frame = +2
Query: 62 YPYQDTTSRAEQFPLIASMLWSIWESRNEIVQ-----EPITILSRAKAMMLN-----STK 211
YP +D Q ++ +LW +W++RNE++ E +++ +A+ + K
Sbjct: 18 YPREDI-----QAEIVPWILWRLWKNRNELLFKGKEFEAGSLILKAREDLEEWQGSCQAK 72
Query: 212 NPFINRTSAITQNQSIPT-PWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
+ A S+ T W PP +WLK N+DGAW+ N E GLG++ RD+S ++A
Sbjct: 73 ITAAEKIEAKRATTSVQTHTWKPPPTDWLKCNSDGAWHQNRETSGLGWICRDASGHVLWA 132
Query: 389 EAK 397
A+
Sbjct: 133 GAR 135
>XP_012088409.1 PREDICTED: uncharacterized protein LOC105647037 [Jatropha curcas]
KDP24067.1 hypothetical protein JCGZ_25724 [Jatropha
curcas]
Length = 194
Score = 60.5 bits (145), Expect = 6e-08
Identities = 35/133 (26%), Positives = 60/133 (45%), Gaps = 6/133 (4%)
Frame = +2
Query: 2 SVTGPIWLTFIQWIRYWMQLYPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPI 166
S + PI F +W M + Q + P ++ W IW++RN P+
Sbjct: 44 SPSNPIAQPFSRWFEVIMDTFYNQ---GNEDLIPNTVALCWQIWKTRNAAYFRQEQPNPL 100
Query: 167 TILSRAKAMMLNSTKNPFINRTSAITQNQSIP-TPWVVPPRNWLKINTDGAWNPNSEIGG 343
T ++RA M+ + PF++ T N + P W PP +LK N D A+ ++++ G
Sbjct: 101 TTIARATKMIQEARTAPFVSATLLPQNNAANPRNTWNPPPFGFLKFNVDAAFKDSTQVAG 160
Query: 344 LGFLIRDSSNEFI 382
+ + R+ E I
Sbjct: 161 IAIICRNDKGELI 173
>XP_015389111.1 PREDICTED: uncharacterized protein LOC107178429 [Citrus sinensis]
Length = 221
Score = 60.5 bits (145), Expect = 8e-08
Identities = 34/100 (34%), Positives = 52/100 (52%), Gaps = 5/100 (5%)
Frame = +2
Query: 104 LIASMLWSIWESRNEIV----QEPITILSRAKAMMLNSTKNPFINRTSAITQNQSI-PTP 268
LI + WSIW SRN ++ +E + A ++ S + I T ++QNQS
Sbjct: 3 LIIATCWSIWSSRNLLIFKGKEEDAQLSVGRAAAVVESYRRIKIPATQTVSQNQSNNQQT 62
Query: 269 WVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
W+ P W K+N D A P+++ GLG +IRDS + + A
Sbjct: 63 WLPPLNGWYKVNVDAAIRPSNQTAGLGVVIRDSRGKVVAA 102
>XP_015387924.1 PREDICTED: uncharacterized protein LOC107177897 [Citrus sinensis]
Length = 219
Score = 60.1 bits (144), Expect = 1e-07
Identities = 32/92 (34%), Positives = 50/92 (54%), Gaps = 5/92 (5%)
Frame = +2
Query: 104 LIASMLWSIWESRNEIVQE-----PITILSRAKAMMLNSTKNPFINRTSAITQNQSIPTP 268
L+A++LW IW +RN+ + E PI ++ RA++++ + K R Q
Sbjct: 53 LVAALLWVIWNARNKWLFEGKKENPIIVVVRAESVVESYRKVKQPERNFVGNQKAEKLNQ 112
Query: 269 WVVPPRNWLKINTDGAWNPNSEIGGLGFLIRD 364
W PP++WLKIN D + N + GLG +IRD
Sbjct: 113 WYPPPKDWLKINVDATVDANKQKAGLGVVIRD 144
>XP_015387369.1 PREDICTED: uncharacterized protein LOC107177677 [Citrus sinensis]
Length = 127
Score = 58.2 bits (139), Expect = 1e-07
Identities = 32/109 (29%), Positives = 57/109 (52%), Gaps = 8/109 (7%)
Frame = +2
Query: 95 QFPLIASMLWSIWESRNEIVQE-----PITILSRAKAMMLNSTKNPFINRT---SAITQN 250
+F L+ + W+IW +N + E P+ L +AKA++ + + ++ + +++ Q
Sbjct: 4 EFELLVVVFWAIWHVKNNFLFESKKVDPLISLVKAKAVLESYKRIKSLSESHLHNSLVQK 63
Query: 251 QSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEAK 397
Q W+ PP+ WLKIN D N + GL + RDS+ +F+ A K
Sbjct: 64 QQ--HQWIPPPQGWLKINVDATRNREKKSAGLRVIARDSTGKFVGAAVK 110
>XP_013639539.1 PREDICTED: uncharacterized protein LOC106344775 [Brassica oleracea
var. oleracea]
Length = 373
Score = 60.8 bits (146), Expect = 1e-07
Identities = 36/118 (30%), Positives = 64/118 (54%), Gaps = 6/118 (5%)
Frame = +2
Query: 68 YQDTTSRAEQFPLIASMLWSIWESRNEIVQEPI-----TILSRAKAMMLNSTKNPFINRT 232
+QD T + L+ +LW IW++RNE + + I +I+S+A+ M + K +T
Sbjct: 133 HQDPTEKVYD-DLVPWLLWRIWKNRNEFLFKGIDYTAPSIVSKAREDMEDWLKRKEEEKT 191
Query: 233 SAITQNQSIPTP-WVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEAKNL 403
A T +S W PP +W+K N+DGAW + G+G++ R+ + ++A K++
Sbjct: 192 VANTAPRSSQHHRWKPPPTSWIKCNSDGAWQRDRHQNGMGWISRNEHGKLLWAGMKSV 249
>XP_013625423.1 PREDICTED: uncharacterized protein LOC106331580 [Brassica oleracea
var. oleracea]
Length = 304
Score = 60.5 bits (145), Expect = 2e-07
Identities = 35/123 (28%), Positives = 58/123 (47%), Gaps = 11/123 (8%)
Frame = +2
Query: 62 YPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPITILSRAKAMMLNSTKNPFIN 226
YP +D Q + ++W +W++RNE+V E I+ +A+
Sbjct: 67 YPIEDI-----QAEIAPWLMWRLWKNRNELVFNGKEYEAECIIRKAREDAEEWKGRKVAE 121
Query: 227 RTSAITQNQSIPT------PWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
T+ PT PW PP NW+K N+DGAW+ + GLG++ R+ + E ++A
Sbjct: 122 ETAVKRLEVQQPTITNQANPWRPPPSNWVKCNSDGAWHNERDTSGLGWICRNETGEVLWA 181
Query: 389 EAK 397
A+
Sbjct: 182 GAR 184
>XP_015388029.1 PREDICTED: uncharacterized protein LOC107177961 [Citrus sinensis]
Length = 225
Score = 59.7 bits (143), Expect = 2e-07
Identities = 30/104 (28%), Positives = 60/104 (57%), Gaps = 7/104 (6%)
Frame = +2
Query: 107 IASMLWSIWESRNEIVQE-----PITILSRAKAMM--LNSTKNPFINRTSAITQNQSIPT 265
+A++LW IW +RN+ + E P+++++RA++++ +++ P N + +++
Sbjct: 42 VAALLWVIWTARNKWLFEGRKDDPLSLMARAESIVEAFRNSRKPLKNFQAKKEDDKA--N 99
Query: 266 PWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEAK 397
W PP+ WLK+N D A + ++ GLG ++RD + I A K
Sbjct: 100 QWCPPPKGWLKVNVDAAVDEKRQVAGLGVVVRDWKSNCIAAAIK 143
>XP_009113118.1 PREDICTED: uncharacterized protein LOC103838431 [Brassica rapa]
Length = 262
Score = 60.1 bits (144), Expect = 2e-07
Identities = 34/112 (30%), Positives = 54/112 (48%), Gaps = 11/112 (9%)
Frame = +2
Query: 95 QFPLIASMLWSIWESRNEIV-----QEPITILSRAKAMMLNSTKNPFINRTSAITQNQSI 259
Q ++ +LW +W+ RNE + E ++L + + + + + A I
Sbjct: 25 QSDIVPWLLWRLWKCRNEFLFKGREYEARSVLRKVQEDVEEWRERKKEEASEAERVGARI 84
Query: 260 PTP------WVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEAK 397
PT W PP NWLK N+DGAWN N GLG+++R+ ++A AK
Sbjct: 85 PTTANQRCVWKPPPANWLKCNSDGAWNHNRVNSGLGWVLRNEKGNVLWAGAK 136
>XP_013601608.1 PREDICTED: uncharacterized protein LOC106309082 [Brassica oleracea
var. oleracea]
Length = 261
Score = 59.7 bits (143), Expect = 2e-07
Identities = 35/123 (28%), Positives = 58/123 (47%), Gaps = 11/123 (8%)
Frame = +2
Query: 62 YPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPITILSRAKAMMLNSTKNPFIN 226
YP +D Q + + ++W +W++RNE+V E IL +A+
Sbjct: 19 YPIEDI-----QAEIASWLMWRLWKNRNELVFNGKEYEAECILRKAREDAEEWKGRKVAE 73
Query: 227 RTSAITQNQSIPT------PWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYA 388
T+ PT PW PP NW+K N+D AW+ + GLG++ R+ + E ++A
Sbjct: 74 ETAVKRLEVQQPTITNQANPWRPPPSNWVKCNSDSAWHNERDTSGLGWICRNETGEVLWA 133
Query: 389 EAK 397
A+
Sbjct: 134 GAR 136
>XP_004298055.1 PREDICTED: uncharacterized protein LOC101313796 [Fragaria vesca
subsp. vesca]
Length = 165
Score = 58.2 bits (139), Expect = 3e-07
Identities = 36/116 (31%), Positives = 53/116 (45%), Gaps = 5/116 (4%)
Frame = +2
Query: 50 WMQLYPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPITILSRAKAMMLNSTKN 214
W + T F + +LWSIW +RNE++ + P I+ A A +
Sbjct: 32 WKDWLLERAVTLTPAMFDKLLVLLWSIWRNRNEVLWRDKGKSPHQIIIAAMAWYEEYLQA 91
Query: 215 PFINRTSAITQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFI 382
++TS+ QS W P LK+N DG + P + GGLG ++RDS FI
Sbjct: 92 NVAHQTSSAVNRQSGRKFWKPPFGTALKLNVDGGFLPTTNQGGLGGVVRDSQGHFI 147
>XP_013594814.1 PREDICTED: uncharacterized protein LOC106302971 [Brassica oleracea
var. oleracea]
Length = 403
Score = 60.1 bits (144), Expect = 3e-07
Identities = 37/124 (29%), Positives = 61/124 (49%), Gaps = 13/124 (10%)
Frame = +2
Query: 62 YPYQDTTSRAEQFPLIASMLWSIWESRNEIV-----QEPITILSRAKAMMLNSTKNPFIN 226
YP + S ++ +LW +W+ RNE++ E ++IL +A+ + +N
Sbjct: 160 YPRDEVKSE-----MVPWLLWRLWKCRNELLFNGKEYEAMSILRKAREDADEWERRKQVN 214
Query: 227 R--------TSAITQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFI 382
T I+ +Q+ PW +PP NWLK N+DGAW+ + G+G+L RD +
Sbjct: 215 LSVEGNKRVTHPISTSQA--KPWKLPPTNWLKCNSDGAWHKDRLNSGVGWLCRDGEGRLL 272
Query: 383 YAEA 394
A A
Sbjct: 273 CAGA 276
>ONI01475.1 hypothetical protein PRUPE_6G141500 [Prunus persica]
Length = 295
Score = 59.7 bits (143), Expect = 3e-07
Identities = 37/129 (28%), Positives = 62/129 (48%), Gaps = 3/129 (2%)
Frame = +2
Query: 26 TFIQWIRYWMQLYPYQDTTSRAEQFPLIASMLWSIWESRNEIVQEPITILSRAKAMMLNS 205
T W+R ++ TS+A++ +I ++LW+IW +RN ++ + +
Sbjct: 42 TMKDWLREMLE------GTSKAQRDHII-TLLWAIWGNRNALLWQGVQRSPHEVVGFAQQ 94
Query: 206 TKNPFINRT---SAITQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNE 376
+ FI S +T P W PP LK N DGA++P S G +G + RD+
Sbjct: 95 YVHEFITANDTPSKVTDRVRDPVRWAAPPSGRLKFNFDGAFDPTSGRGAVGVVARDADGG 154
Query: 377 FIYAEAKNL 403
F+ A AK++
Sbjct: 155 FVAAVAKSV 163
>XP_007206484.1 hypothetical protein PRUPE_ppa019056mg, partial [Prunus persica]
Length = 298
Score = 59.7 bits (143), Expect = 3e-07
Identities = 37/129 (28%), Positives = 62/129 (48%), Gaps = 3/129 (2%)
Frame = +2
Query: 26 TFIQWIRYWMQLYPYQDTTSRAEQFPLIASMLWSIWESRNEIVQEPITILSRAKAMMLNS 205
T W+R ++ TS+A++ +I ++LW+IW +RN ++ + +
Sbjct: 45 TMKDWLREMLE------GTSKAQRDHII-TLLWAIWGNRNALLWQGVQRSPHEVVGFAQQ 97
Query: 206 TKNPFINRT---SAITQNQSIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNE 376
+ FI S +T P W PP LK N DGA++P S G +G + RD+
Sbjct: 98 YVHEFITANDTPSKVTDRVRDPVRWAAPPSGRLKFNFDGAFDPTSGRGAVGVVARDADGG 157
Query: 377 FIYAEAKNL 403
F+ A AK++
Sbjct: 158 FVAAVAKSV 166
>XP_009368150.1 PREDICTED: uncharacterized protein LOC103957683 [Pyrus x
bretschneideri]
Length = 241
Score = 58.9 bits (141), Expect = 4e-07
Identities = 32/107 (29%), Positives = 52/107 (48%), Gaps = 5/107 (4%)
Frame = +2
Query: 89 AEQFPLIASMLWSIWESRNEIVQE-----PITILSRAKAMMLNSTKNPFINRTSAITQNQ 253
A F L +++WS+W +RNE++ P I+ +A+ + + N + Q
Sbjct: 19 ASGFELCLTLIWSLWHNRNEVLWNGKPLNPFEIVLQAEGWLQEFHRCDKTNAKKGRRELQ 78
Query: 254 SIPTPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFIYAEA 394
W P W+K N DGAW P+ + GG G +IR+ +F+ A A
Sbjct: 79 K----WSKPDEGWVKCNFDGAWIPSQKRGGFGVVIRNQVGDFLGAAA 121
>XP_010490159.1 PREDICTED: uncharacterized protein LOC104767890 [Camelina sativa]
Length = 1712
Score = 60.1 bits (144), Expect = 4e-07
Identities = 35/129 (27%), Positives = 63/129 (48%), Gaps = 12/129 (9%)
Frame = +2
Query: 59 LYPYQDTTSRAEQFPLIASMLWSIWESRNEIVQE-----PITILSRA--KAMMLNSTKNP 217
LYP +D + + +LW +W+SRNE++ + P + A A + K
Sbjct: 1476 LYPQRDDLDKD-----VPWVLWRLWKSRNELIFKGKDFNPQNTVETALEDAEEWDQRKK- 1529
Query: 218 FINRTSAITQNQSIP-----TPWVVPPRNWLKINTDGAWNPNSEIGGLGFLIRDSSNEFI 382
N +T+ +P W +PPR W+K NTDGAW + G G+++R+++ + +
Sbjct: 1530 --NEHEKLTRGHPVPKRTEDAKWKLPPREWVKCNTDGAWRGEDTLSGTGWVLRNNTGDVL 1587
Query: 383 YAEAKNLVC 409
+ A+ + C
Sbjct: 1588 WMGAQAIRC 1596