BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver32_contig00023082
         (670 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ADB89213.1 cheilanthifoline synthase [Papaver somniferum]             163   7e-68
B1NF20.1 RecName: Full=Cheilanthifoline synthase; Short=CHS; Alt...   167   1e-67
ANY58175.1 putative CYP719 [synthetic construct]                      160   7e-67
ANY58179.1 putative CYP719 [synthetic construct]                      169   7e-67
ACO90230.1 cheilanthifoline synthase-like protein, partial [Papa...   159   2e-66
B5UAQ8.1 RecName: Full=Cheilanthifoline synthase; Short=CHS; Alt...   166   1e-64
ANY58158.1 putative CYP719 [synthetic construct]                      163   2e-62
ANY58184.1 putative CYP719 [synthetic construct]                      153   1e-60
ANY58148.1 putative CYP719 [synthetic construct]                      154   2e-60
ANY58183.1 putative CYP719 [synthetic construct]                      150   5e-60
ANY58182.1 putative CYP719 [synthetic construct]                      154   1e-59
ANY58161.1 putative CYP719 [synthetic construct]                      137   6e-57
ANY58165.1 putative CYP719 [synthetic construct]                      136   2e-56
ANY58189.1 putative N-methyltransferase [synthetic construct]         139   2e-56
AGL76711.1 (S)-canadine synthase, partial [Coptis chinensis]          142   3e-56
ANY58180.1 putative CYP719 [synthetic construct]                      142   4e-56
ACO90219.1 stylopine synthase [Eschscholzia californica] ANY5815...   144   5e-56
Q948Y1.1 RecName: Full=(S)-canadine synthase; AltName: Full=(S)-...   140   5e-56
ANY58170.1 SLS [synthetic construct]                                  150   7e-56
AAU20771.1 (S)-canadine synthase [Thalictrum flavum subsp. glauc...   137   7e-56

>ADB89213.1 cheilanthifoline synthase [Papaver somniferum]
          Length = 492

 Score =  163 bits (413), Expect(2) = 7e-68
 Identities = 85/151 (56%), Positives = 107/151 (70%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  + NI+  D GPFW NLRKGLQ VAL P NV SQ HLQER M  LIKSM++K
Sbjct: 107 DITKIISANWKNISHGDSGPFWHNLRKGLQGVALTPFNVASQYHLQERDMQNLIKSMKKK 166

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A Q+NGILKPLDY+KEE++RLLS LIFGQDF DE+FVV M++ L +LV I    SL+DAF
Sbjct: 167 ASQKNGILKPLDYVKEETVRLLSRLIFGQDFVDEDFVVGMHQALDELVSISGYASLADAF 226

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYI 658
              + LPS KK +R +  +  +  +LI P+I
Sbjct: 227 KFCENLPSHKKTVRAVHAIKIRFDNLIRPHI 257



 Score =  122 bits (306), Expect(2) = 7e-68
 Identities = 55/65 (84%), Positives = 61/65 (93%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G+ FHV +AN AKV+GG+FT+W+GSWRPFIVISDVDKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGEAFHVCLANLAKVYGGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDI 197
           DMPDI
Sbjct: 104 DMPDI 108


>B1NF20.1 RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A14 ABR14722.1 cheilanthifoline
           synthase [Argemone mexicana] ANY58136.1 CYP719A14
           [synthetic construct]
          Length = 494

 Score =  167 bits (423), Expect(2) = 1e-67
 Identities = 89/151 (58%), Positives = 107/151 (70%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  + NI+ SD GPFW NLRKGLQ VAL PLNV SQ HLQER M  LI SM + 
Sbjct: 109 DITKIISANWKNISCSDSGPFWHNLRKGLQGVALTPLNVASQYHLQERDMKNLINSMYKD 168

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A ++NGILKPLDYLKEE++RLLS LIFGQDF DE  VV M+  L DLVRI    SL+DAF
Sbjct: 169 ASRKNGILKPLDYLKEETVRLLSRLIFGQDFQDEKLVVGMHHALDDLVRISGYASLADAF 228

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYI 658
              + LPS KK IRE+ +V ++  +LI P+I
Sbjct: 229 KFCENLPSHKKSIREVHEVKKRVENLIRPHI 259



 Score =  117 bits (294), Expect(2) = 1e-67
 Identities = 51/65 (78%), Positives = 59/65 (90%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL GD FHV +AN AKV+G +FT+W+GSWRP I++SD+DKAWEVLVNKSSDYSAR
Sbjct: 46  IGNLHQLGGDVFHVVLANLAKVYGSVFTIWVGSWRPMIIVSDIDKAWEVLVNKSSDYSAR 105

Query: 183 DMPDI 197
           DMPDI
Sbjct: 106 DMPDI 110


>ANY58175.1 putative CYP719 [synthetic construct]
          Length = 492

 Score =  160 bits (404), Expect(2) = 7e-67
 Identities = 84/151 (55%), Positives = 106/151 (70%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  + NI+  D GPFW NLRKGLQ VAL P NV SQ HLQER M  LIKSM++K
Sbjct: 107 DITKIISANWKNISHGDSGPFWHNLRKGLQGVALTPFNVASQYHLQERDMQNLIKSMKKK 166

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A Q+NGILKPLDY+KEE++RLLS LIF Q+F DE+FVV M++ L +LV I    SL+DAF
Sbjct: 167 ASQKNGILKPLDYVKEETVRLLSRLIFSQEFVDEDFVVGMHQALDELVSISGYASLADAF 226

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYI 658
              + LPS KK IR +  +  +  +LI P+I
Sbjct: 227 KFCENLPSHKKTIRAVHAIKIRFDNLIRPHI 257



 Score =  122 bits (306), Expect(2) = 7e-67
 Identities = 55/65 (84%), Positives = 61/65 (93%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G+ FHV +AN AKV+GG+FT+W+GSWRPFIVISDVDKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGEAFHVCLANLAKVYGGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDI 197
           DMPDI
Sbjct: 104 DMPDI 108


>ANY58179.1 putative CYP719 [synthetic construct]
          Length = 485

 Score =  169 bits (427), Expect(2) = 7e-67
 Identities = 89/151 (58%), Positives = 108/151 (71%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  + NI+ SD GPFW NLRKGLQ  AL PLNV SQ HLQER M  LI SMQQ+
Sbjct: 106 DITKIISANWKNISCSDSGPFWHNLRKGLQGGALAPLNVISQYHLQERDMQNLINSMQQE 165

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A + NGILKPLD+LK+E++RLLS LIFGQDF DE  VV M+  L DLVRI    SL+DAF
Sbjct: 166 ASKNNGILKPLDHLKQETVRLLSRLIFGQDFHDEKLVVGMHHALDDLVRISGYASLADAF 225

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYI 658
             A+ LPS KK IRE+ +V E+  +L+ P+I
Sbjct: 226 KFAENLPSHKKSIREVHEVKERVENLVRPHI 256



 Score =  113 bits (283), Expect(2) = 7e-67
 Identities = 49/65 (75%), Positives = 58/65 (89%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL GD FHV +AN AKV+G + T+W+GSWRP I++SD+DKAWEVLV+KSSDYSAR
Sbjct: 43  IGNLHQLGGDVFHVVLANLAKVYGSVMTIWVGSWRPMIIVSDIDKAWEVLVSKSSDYSAR 102

Query: 183 DMPDI 197
           DMPDI
Sbjct: 103 DMPDI 107


>ACO90230.1 cheilanthifoline synthase-like protein, partial [Papaver
           bracteatum]
          Length = 271

 Score =  159 bits (401), Expect(2) = 2e-66
 Identities = 82/151 (54%), Positives = 106/151 (70%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  + NI+  D  PFW NLRKGLQ VAL P NV +Q HLQER M  LIKSM++K
Sbjct: 107 DITKIISANWKNISHGDSSPFWHNLRKGLQGVALTPFNVAAQHHLQERDMQNLIKSMKEK 166

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A Q+NGILKPLDY+KEE++RLLS LIFGQ+F DE+FVV M++ L +LV I    SL+DAF
Sbjct: 167 ASQKNGILKPLDYVKEETVRLLSRLIFGQEFVDEDFVVGMHQALDELVSISGYASLADAF 226

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYI 658
              + LPS KK +R +  +  +  +LI P+I
Sbjct: 227 KFCENLPSHKKTVRAVHAIKIRFDNLIRPHI 257



 Score =  122 bits (306), Expect(2) = 2e-66
 Identities = 55/65 (84%), Positives = 61/65 (93%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G+ FHV +AN AKV+GG+FT+W+GSWRPFIVISDVDKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGEAFHVCLANLAKVYGGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDI 197
           DMPDI
Sbjct: 104 DMPDI 108


>B5UAQ8.1 RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A5 BAG75113.1 cheilanthifoline
           synthase [Eschscholzia californica] ANY58153.1 CYP719A5
           [synthetic construct]
          Length = 490

 Score =  166 bits (419), Expect(2) = 1e-64
 Identities = 88/142 (61%), Positives = 104/142 (73%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI+ SD GPFW NLRKGLQ  AL PLNV SQ  LQER M  LI SMQ+KA + NGILK
Sbjct: 115 WKNISCSDSGPFWQNLRKGLQGGALAPLNVISQYQLQERDMKNLITSMQEKASKNNGILK 174

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLDYLKEE+IRLLS LIFGQ F+DENFV  ++  L DLVRI    SL+DAF   + LPS 
Sbjct: 175 PLDYLKEETIRLLSRLIFGQSFNDENFVKGVHLALDDLVRISGYASLADAFKFCENLPSH 234

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ +V E+  +L+ PY+
Sbjct: 235 KKSIREVHEVNERVVNLVKPYL 256



 Score =  108 bits (271), Expect(2) = 1e-64
 Identities = 46/65 (70%), Positives = 56/65 (86%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G+ FHV +AN AK+HG + T+W+G+WRP IVISD+DKAWEVLVNKSSDY+ R
Sbjct: 43  IGNLHQLGGEAFHVVLANLAKIHGTVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYAGR 102

Query: 183 DMPDI 197
           D P+I
Sbjct: 103 DFPEI 107


>ANY58158.1 putative CYP719 [synthetic construct]
          Length = 500

 Score =  163 bits (413), Expect(2) = 2e-62
 Identities = 85/142 (59%), Positives = 105/142 (73%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI+ SD GPFWTNLRKGLQ  AL PLNV SQ HLQE+ M  L+KSM+ +A + NG +K
Sbjct: 125 WKNISCSDSGPFWTNLRKGLQGGALAPLNVISQYHLQEKDMENLVKSMRVEASKNNGNIK 184

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+E++RLLS LIFGQDF DE  VV M+  L DLVRI    SL+DAF IA+ LPS 
Sbjct: 185 PLDHLKQETVRLLSRLIFGQDFHDEKLVVGMHHALDDLVRISGYASLADAFKIAENLPSH 244

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ +V E+   L+ P+I
Sbjct: 245 KKSIREVHEVNERVKKLVRPHI 266



 Score =  104 bits (259), Expect(2) = 2e-62
 Identities = 45/65 (69%), Positives = 54/65 (83%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G+ FHV +AN AKVHG + T+W+GSWRP IVISD++KAWE+LV KSSDYS R
Sbjct: 53  IGNLHQLGGNVFHVVLANLAKVHGSVMTIWVGSWRPMIVISDIEKAWEMLVTKSSDYSGR 112

Query: 183 DMPDI 197
             P+I
Sbjct: 113 SFPEI 117


>ANY58184.1 putative CYP719 [synthetic construct]
          Length = 477

 Score =  153 bits (386), Expect(2) = 1e-60
 Identities = 80/142 (56%), Positives = 102/142 (71%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI  SD GPFW NLRKGLQ  AL P NV SQ  LQER M  LIK+M+ +A + NG +K
Sbjct: 116 WKNIMTSDSGPFWQNLRKGLQGGALSPHNVISQYQLQERDMQHLIKTMRVEASKNNGRIK 175

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+E++RLLS LIFGQDF+DE  VV M+  L DLVRI    SL+DAF  A+ LPS 
Sbjct: 176 PLDHLKQETVRLLSRLIFGQDFNDEKLVVGMHHALDDLVRISGYASLADAFKFAENLPSH 235

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ ++ ++  +L+ P+I
Sbjct: 236 KKSIREVHELKKRIENLVRPHI 257



 Score =  108 bits (270), Expect(2) = 1e-60
 Identities = 50/80 (62%), Positives = 62/80 (77%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G  FHV +AN AK++G + T+W+G+WRP IVISD+DKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGAAFHVVLANLAKIYGSVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDIILKTSIQPEMRNILT 242
            +P+I     I    +NI+T
Sbjct: 104 SLPEI--TRIISANWKNIMT 121


>ANY58148.1 putative CYP719 [synthetic construct]
          Length = 490

 Score =  154 bits (389), Expect(2) = 2e-60
 Identities = 80/142 (56%), Positives = 102/142 (71%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI  SD GPFW NLRKGLQ  AL P NV SQ  LQER M  LIK+M+ +A + NG +K
Sbjct: 116 WKNIMTSDSGPFWQNLRKGLQGGALSPHNVMSQYQLQERDMQNLIKTMRVEASKNNGRIK 175

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+E++RLLS LIFGQDF+DE  VV M+  L DLVRI    SL+DAF +A+ LPS 
Sbjct: 176 PLDHLKQETVRLLSRLIFGQDFNDEKLVVGMHHALDDLVRISGYASLADAFKVAENLPSH 235

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ ++  +  +L+ P+I
Sbjct: 236 KKSIREVHELKRRVENLVRPHI 257



 Score =  106 bits (265), Expect(2) = 2e-60
 Identities = 50/80 (62%), Positives = 61/80 (76%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G   HV +AN AKV+G + T+W+G+WRP IVISD+DKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGAALHVVLANLAKVYGSVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDIILKTSIQPEMRNILT 242
            +P+I     I    +NI+T
Sbjct: 104 SLPEI--TRIISANWKNIMT 121


>ANY58183.1 putative CYP719 [synthetic construct]
          Length = 490

 Score =  150 bits (380), Expect(2) = 5e-60
 Identities = 79/142 (55%), Positives = 100/142 (70%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI  SD GPFW NLRKGLQ  AL P NV SQ  LQER M  LIK+++ +A   NG +K
Sbjct: 116 WKNIMTSDSGPFWQNLRKGLQGGALSPHNVISQYQLQERDMQNLIKTIRVEASTNNGRIK 175

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+E++RLLS LIFGQDF DE  VV M+  L DLVRI    SL+DAF  A+ LPS 
Sbjct: 176 PLDHLKQETVRLLSRLIFGQDFKDEKLVVGMHHALDDLVRISGYASLADAFKFAENLPSH 235

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ ++ ++  +L+ P+I
Sbjct: 236 KKSIREVHELKKRVENLVRPHI 257



 Score =  108 bits (271), Expect(2) = 5e-60
 Identities = 51/80 (63%), Positives = 62/80 (77%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G  FHV +AN AKV+G + T+W+G+WRP IVISD+DKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGAAFHVVLANLAKVYGSVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDIILKTSIQPEMRNILT 242
            +P+I     I    +NI+T
Sbjct: 104 SLPEI--TRIISANWKNIMT 121


>ANY58182.1 putative CYP719 [synthetic construct]
          Length = 490

 Score =  154 bits (388), Expect(2) = 1e-59
 Identities = 81/142 (57%), Positives = 103/142 (72%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           + NI  SD GPFW NLRKGLQS AL P NV SQ  LQER M  LIK+M+ +A + NG +K
Sbjct: 116 WKNIMTSDSGPFWQNLRKGLQSGALSPHNVMSQYQLQERDMQNLIKTMRVEAFKNNGRIK 175

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+E++RLLS LIFGQDF+DE  VV M+  L DLVRI    SL+DAF  A+ LPS 
Sbjct: 176 PLDHLKQETVRLLSRLIFGQDFNDEKLVVGMHHALDDLVRISGYASLADAFKFAENLPSH 235

Query: 593 KKVIRELDDVMEQAASLIIPYI 658
           KK IRE+ ++ ++  +L+ P+I
Sbjct: 236 KKSIREVHELKKRVENLVRPHI 257



 Score =  104 bits (260), Expect(2) = 1e-59
 Identities = 50/80 (62%), Positives = 59/80 (73%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL G   HV +AN AK +G + T+W+GSWRP IVISD DKAWEVLVNKSSDYSAR
Sbjct: 44  IGNLHQLGGAALHVVLANLAKAYGSVMTIWVGSWRPMIVISDFDKAWEVLVNKSSDYSAR 103

Query: 183 DMPDIILKTSIQPEMRNILT 242
            +P+I     I    +NI+T
Sbjct: 104 SLPEI--TRIISANWKNIMT 121


>ANY58161.1 putative CYP719 [synthetic construct]
          Length = 492

 Score =  137 bits (344), Expect(2) = 6e-57
 Identities = 66/154 (42%), Positives = 103/154 (66%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++   G+  +  I+ SD GPFW N+RKGLQ  A+GPLN+++QT  QE+ M  LI++M+ +
Sbjct: 112 EITKIGSASWHTISTSDPGPFWQNVRKGLQGGAMGPLNMSAQTPFQEKDMKNLIETMKIE 171

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A   NGI+KPLD++K+ ++RLLS LIFGQ F D+ F+  M+  + D++RI     L++AF
Sbjct: 172 AENNNGIIKPLDHIKKNTVRLLSRLIFGQMFEDDKFIESMHYEIEDIIRISGYARLAEAF 231

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYISTS 667
           + AKYLPS K+  +E   V  +   L+ P + ++
Sbjct: 232 YYAKYLPSHKRAEKEAFLVKCRVEDLVRPLLKSN 265



 Score =  112 bits (280), Expect(2) = 6e-57
 Identities = 49/65 (75%), Positives = 56/65 (86%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGN+HQL GD  HV +   +K+HGG+FT WIGSWRP IVISD+DKAWEVLVNKSSDYSAR
Sbjct: 49  IGNMHQLGGDVLHVALDKLSKIHGGVFTFWIGSWRPVIVISDIDKAWEVLVNKSSDYSAR 108

Query: 183 DMPDI 197
           DMP+I
Sbjct: 109 DMPEI 113


>ANY58165.1 putative CYP719 [synthetic construct]
          Length = 501

 Score =  136 bits (343), Expect(2) = 2e-56
 Identities = 65/154 (42%), Positives = 104/154 (67%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++    +  + NI+ S+ GPFW +LRKGLQ+  LGPLN+++Q   QER M R+I  MQ++
Sbjct: 110 EITKIASAGWRNISSSNSGPFWHSLRKGLQNGPLGPLNISAQIFSQERDMKRMIHDMQEE 169

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A    GI+KPLD+LK+ S+RLL+ LIFGQ+F D++FV  ++    +L+RI     L++AF
Sbjct: 170 AAMNGGIVKPLDHLKKASVRLLNRLIFGQNFDDDSFVESIHHETEELIRISGYARLAEAF 229

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYISTS 667
             AKYLPS ++ ++E  +  ++   L+ P +S++
Sbjct: 230 SYAKYLPSHRRAVKEAYEGKQRVEELVRPLLSSN 263



 Score =  111 bits (277), Expect(2) = 2e-56
 Identities = 52/65 (80%), Positives = 55/65 (84%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL GD  HV +A  AKVHG I T+WIGSWRP IVISD DKAWEVLVNKSSDYSAR
Sbjct: 47  IGNLHQLGGDVLHVVLAKLAKVHGNIMTIWIGSWRPVIVISDYDKAWEVLVNKSSDYSAR 106

Query: 183 DMPDI 197
           DMP+I
Sbjct: 107 DMPEI 111


>ANY58189.1 putative N-methyltransferase [synthetic construct]
          Length = 492

 Score =  139 bits (351), Expect(2) = 2e-56
 Identities = 68/154 (44%), Positives = 100/154 (64%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++    +  +  I+ SD GPFW N+RKGLQS A+GPLNV +Q   QER M RL+ +M  +
Sbjct: 110 EITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPLNVAAQNQFQERDMKRLVNAMSDE 169

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A    GI+KPLD++K+ ++RLL+ LIFGQ F D+ F+  M+  + D++RI     L++AF
Sbjct: 170 ALNSKGIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFIESMHYEIEDIIRISGYARLAEAF 229

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYISTS 667
           + AKYLPS KK  RE   V  +   L+ P +S++
Sbjct: 230 YYAKYLPSHKKAEREAFLVKCRVEKLVRPLLSSN 263



 Score =  107 bits (268), Expect(2) = 2e-56
 Identities = 48/65 (73%), Positives = 54/65 (83%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL  D  HV +A  AKVHG + T+WIGSWRP IVISD+DKAWEVLVNKS+DY AR
Sbjct: 47  IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDIDKAWEVLVNKSADYGAR 106

Query: 183 DMPDI 197
           DMP+I
Sbjct: 107 DMPEI 111


>AGL76711.1 (S)-canadine synthase, partial [Coptis chinensis]
          Length = 491

 Score =  142 bits (358), Expect(2) = 3e-56
 Identities = 70/154 (45%), Positives = 101/154 (65%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++    +  +  I+ SD GPFW N+RKGLQS A+GP NV +Q   QER M RLIK+M  +
Sbjct: 109 EITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPFNVAAQNQHQERGMKRLIKAMSDE 168

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A   NGI+KPLD++K+ ++RLL+ LIFGQ+F D  F+  M+  + D++RI     L++AF
Sbjct: 169 AANNNGIVKPLDHIKKNTVRLLTRLIFGQNFDDNKFIESMHYEIEDIIRISGYARLAEAF 228

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYISTS 667
           + AKYLPS KK  RE   V  +   L+ P +S++
Sbjct: 229 YYAKYLPSHKKAEREAFLVKCRVQELVRPLLSSN 262



 Score =  104 bits (260), Expect(2) = 3e-56
 Identities = 47/65 (72%), Positives = 54/65 (83%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL  D  HV +A  AKVHG + T+WIGSWRP IVISD++KAWEVLVNKS+DY AR
Sbjct: 46  IGNLHQLGDDVLHVALAKLAKVHGSMMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGAR 105

Query: 183 DMPDI 197
           DMP+I
Sbjct: 106 DMPEI 110


>ANY58180.1 putative CYP719 [synthetic construct]
          Length = 496

 Score =  142 bits (357), Expect(2) = 4e-56
 Identities = 62/145 (42%), Positives = 106/145 (73%)
 Frame = +2

Query: 233 YTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILK 412
           +  I+ SD GPFWTNLRKGLQ+VAL P N+ +Q   QER + ++I++++++A   NGI+K
Sbjct: 121 WKTISTSDSGPFWTNLRKGLQNVALSPHNLAAQARFQERDISKMIETLKEEAALNNGIVK 180

Query: 413 PLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSS 592
           PLD+LK+ ++RL+S LI+GQ+F +  +V  M+  + +L+R+     L++AF+ AKYLPS 
Sbjct: 181 PLDHLKKATVRLISRLIYGQEFDNNKYVEDMHHAIEELIRVSGYARLAEAFYYAKYLPSH 240

Query: 593 KKVIRELDDVMEQAASLIIPYISTS 667
           KK +R++++   +  +L+ P++S++
Sbjct: 241 KKAVRDVEEANRRVKNLVRPFLSSN 265



 Score =  104 bits (260), Expect(2) = 4e-56
 Identities = 45/65 (69%), Positives = 55/65 (84%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGN+H L G   HV + NFAK++G + T+WIGSWRP IV+SD+D+AWEVLVNKSSDYSAR
Sbjct: 49  IGNMHLLGGTALHVVLHNFAKIYGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSAR 108

Query: 183 DMPDI 197
           DMP+I
Sbjct: 109 DMPEI 113


>ACO90219.1 stylopine synthase [Eschscholzia californica] ANY58155.1 CYP719A2
           [synthetic construct]
          Length = 495

 Score =  144 bits (363), Expect(2) = 5e-56
 Identities = 64/152 (42%), Positives = 108/152 (71%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           D+    +  +  I+ SD GP WTNLRKGLQ+VAL P N+ +Q   QE+ M ++I+++Q++
Sbjct: 112 DITKIISADWKTISTSDSGPHWTNLRKGLQNVALSPHNLAAQFQFQEKDMTKMIQTLQEE 171

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A   NGI+KPLD++K+ ++RL+S L+FGQDF+++ +V  M+  + +L+R+     L++AF
Sbjct: 172 ARNNNGIVKPLDHMKKATLRLISRLVFGQDFNNDKYVDDMHLAIEELIRVSGYARLAEAF 231

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYIS 661
           + AKYLPS KK +RE+++   +  +L+ P++S
Sbjct: 232 YYAKYLPSHKKAVREVEEAQRRVQNLVSPFLS 263



 Score =  102 bits (253), Expect(2) = 5e-56
 Identities = 45/65 (69%), Positives = 53/65 (81%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGN+H L G    V + N AKVHG + T+WIGSWRP IV+SD+++AWEVLVNKSSDYSAR
Sbjct: 49  IGNMHLLGGTALQVVLHNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSAR 108

Query: 183 DMPDI 197
           DMPDI
Sbjct: 109 DMPDI 113


>Q948Y1.1 RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 BAB68769.1 methylenedioxy
           bridge-forming enzyme [Coptis japonica] ANY58144.1
           CYP719A1 [synthetic construct]
          Length = 491

 Score =  140 bits (352), Expect(2) = 5e-56
 Identities = 70/153 (45%), Positives = 99/153 (64%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++    +  +  I+ SD G FW N+RKGLQS A+GPLNV +Q   QER M RLIK+M  +
Sbjct: 109 EITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDE 168

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A   NGI+KPLD++K+ ++RLL+ LIFGQ F D  F+  M+  + D++RI     L++AF
Sbjct: 169 AANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAF 228

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYIST 664
           + AKYLPS KK  RE   V  +   L+ P +S+
Sbjct: 229 YYAKYLPSHKKAEREAFLVKCRVEELVRPLLSS 261



 Score =  106 bits (264), Expect(2) = 5e-56
 Identities = 47/65 (72%), Positives = 54/65 (83%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL  D  HV +A  AKVHG + T+WIGSWRP IVISD++KAWEVLVNKS+DY AR
Sbjct: 46  IGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGAR 105

Query: 183 DMPDI 197
           DMP+I
Sbjct: 106 DMPEI 110


>ANY58170.1 SLS [synthetic construct]
          Length = 506

 Score =  150 bits (380), Expect(2) = 7e-56
 Identities = 79/140 (56%), Positives = 104/140 (74%)
 Frame = +2

Query: 239 NIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQKAPQQNGILKPL 418
           NIA+ D GPFW +L+KGLQS  + P NV S + LQE+ M  LIKSMQ++A QQNGILKPL
Sbjct: 125 NIADCDSGPFWHHLKKGLQS-CINPSNVMSLSRLQEKDMQNLIKSMQERASQQNGILKPL 183

Query: 419 DYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAFHIAKYLPSSKK 598
           D+ KE SIRLLS +IFGQDFS+E+ V+ +   L ++VRI  + SL+DAF IAKYLPS +K
Sbjct: 184 DHAKEASIRLLSRVIFGQDFSNEDLVIGVKDALDEMVRISGLASLADAFKIAKYLPSQRK 243

Query: 599 VIRELDDVMEQAASLIIPYI 658
            IR++    ++  +LI P+I
Sbjct: 244 NIRDMYATRDRVYNLIQPHI 263



 Score = 95.1 bits (235), Expect(2) = 7e-56
 Identities = 46/67 (68%), Positives = 54/67 (80%), Gaps = 1/67 (1%)
 Frame = +3

Query: 3   IGNLHQLRGD-DFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSA 179
           IGNLHQL G     V +AN +KV+GG FT+WIGSW P IVISD+D A EVLVNKS+DYSA
Sbjct: 50  IGNLHQLGGGVPLQVALANLSKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSA 109

Query: 180 RDMPDII 200
           RD+PDI+
Sbjct: 110 RDVPDIL 116


>AAU20771.1 (S)-canadine synthase [Thalictrum flavum subsp. glaucum] ANY58187.1
           CYP719A4 [synthetic construct]
          Length = 492

 Score =  137 bits (344), Expect(2) = 7e-56
 Identities = 67/154 (43%), Positives = 102/154 (66%)
 Frame = +2

Query: 206 DVNSAGNEKYTNIAESDVGPFWTNLRKGLQSVALGPLNVTSQTHLQERSMHRLIKSMQQK 385
           ++    +  +  I+ SD G FW  +RKGLQS A+GPLN+ +Q+  QER M RLI++++ +
Sbjct: 110 EITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQERDMKRLIQALRDE 169

Query: 386 APQQNGILKPLDYLKEESIRLLSVLIFGQDFSDENFVVRMNRILYDLVRIDQITSLSDAF 565
           A Q N I+KPLD++K+ ++RLL+ LIFGQ F D+ FV  M+  + D++RI     L++AF
Sbjct: 170 AAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVESMHFEIDDIIRISGYARLAEAF 229

Query: 566 HIAKYLPSSKKVIRELDDVMEQAASLIIPYISTS 667
           + AKYLPS KK  RE   V  +   L+ P +S++
Sbjct: 230 YYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSN 263



 Score =  108 bits (271), Expect(2) = 7e-56
 Identities = 48/65 (73%), Positives = 55/65 (84%)
 Frame = +3

Query: 3   IGNLHQLRGDDFHVRMANFAKVHGGIFTLWIGSWRPFIVISDVDKAWEVLVNKSSDYSAR 182
           IGNLHQL GD  HV +A  A+VHG + T+WIG+WRP IVISD+DKAWEVLVNKSSDY AR
Sbjct: 47  IGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVLVNKSSDYGAR 106

Query: 183 DMPDI 197
           DMP+I
Sbjct: 107 DMPEI 111