BLASTX nr result
ID: Papaver32_contig00010071
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00010071
(459 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine N-m... 149 4e-41
ANY58198.1 putative N-methyltransferase [synthetic construct] 146 6e-40
C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 126 3e-32
ANY58200.1 putative N-methyltransferase [synthetic construct] 125 5e-32
Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 123 6e-31
ANY58190.1 putative N-methyltransferase [synthetic construct] 123 6e-31
ANY58193.1 putative N-methyltransferase [synthetic construct] 111 1e-26
ANY58201.1 putative N-methyltransferase [synthetic construct] 109 8e-26
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 106 1e-24
ANY58195.1 TNMT [synthetic construct] 105 2e-24
ANY58191.1 putative N-methyltransferase [synthetic construct] 98 2e-21
ANY58196.1 putative N-methyltransferase [synthetic construct] 93 1e-19
ANY58192.1 putative N-methyltransferase [synthetic construct] 91 1e-18
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica] 70 4e-11
ANY58202.1 putative N-methyltransferase [synthetic construct] 69 4e-11
AOR51553.1 putative N-methyltransferase [Papaver somniferum] 69 7e-11
Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short... 67 3e-10
ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata] 66 6e-10
AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase,... 65 2e-09
XP_002870227.1 predicted protein [Arabidopsis lyrata subsp. lyra... 60 3e-09
>C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 ACO90236.1 putative
N-methyltransferase [Papaver bracteatum] ANY58199.1
TNMT2 [synthetic construct]
Length = 358
Score = 149 bits (377), Expect = 4e-41
Identities = 69/73 (94%), Positives = 71/73 (97%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMHFART+DAWRKKLDKNMEAVKEILLPGLGGN+EAVNGVITHIRTCCVGGYVQFS NDG
Sbjct: 286 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 345
Query: 278 DEWMNAHLLFKKK 240
DEWMNA LLFKKK
Sbjct: 346 DEWMNAQLLFKKK 358
>ANY58198.1 putative N-methyltransferase [synthetic construct]
Length = 359
Score = 146 bits (369), Expect = 6e-40
Identities = 67/73 (91%), Positives = 71/73 (97%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMHFART+DAWRKKLDKNMEAVKEILLPGLGGN+EAVNGVITHI+TCCVGGYVQFS +DG
Sbjct: 286 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIKTCCVGGYVQFSLSDG 345
Query: 278 DEWMNAHLLFKKK 240
DEWMNA LLFKKK
Sbjct: 346 DEWMNAQLLFKKK 358
>C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 ACO90237.1 tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 126 bits (317), Expect = 3e-32
Identities = 58/73 (79%), Positives = 64/73 (87%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR++D WRK LDKNMEA KEILLPGLGG++EAVNGV+THIRT C+GGY QFS NDG
Sbjct: 286 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDG 345
Query: 278 DEWMNAHLLFKKK 240
DEWM A LLFKKK
Sbjct: 346 DEWMVAQLLFKKK 358
>ANY58200.1 putative N-methyltransferase [synthetic construct]
Length = 358
Score = 125 bits (315), Expect = 5e-32
Identities = 57/73 (78%), Positives = 64/73 (87%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+ DAWRKK+DKNME KEILLPGLGG++EAVN V+THIRT C+GGY QFS N+G
Sbjct: 286 GMHTARSFDAWRKKVDKNMEVAKEILLPGLGGSHEAVNAVVTHIRTFCMGGYEQFSLNNG 345
Query: 278 DEWMNAHLLFKKK 240
DEWM AHLLFKKK
Sbjct: 346 DEWMVAHLLFKKK 358
>Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT AAY79177.1
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 123 bits (308), Expect = 6e-31
Identities = 56/73 (76%), Positives = 63/73 (86%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR++D WRK LDKNMEA KEILLPGLGG++E VNGV+THIRT C+GGY QFS N+G
Sbjct: 286 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNG 345
Query: 278 DEWMNAHLLFKKK 240
DEWM A LLFKKK
Sbjct: 346 DEWMVAQLLFKKK 358
>ANY58190.1 putative N-methyltransferase [synthetic construct]
Length = 362
Score = 123 bits (308), Expect = 6e-31
Identities = 54/73 (73%), Positives = 66/73 (90%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+++AWRKKLDKNM+A KEILLPGLGGN+EAVN ++THIRT C+GGY QFS+N+G
Sbjct: 290 GMHMARSVEAWRKKLDKNMKAAKEILLPGLGGNHEAVNQIVTHIRTFCMGGYEQFSYNNG 349
Query: 278 DEWMNAHLLFKKK 240
+EWM A +LFKKK
Sbjct: 350 EEWMVAQMLFKKK 362
>ANY58193.1 putative N-methyltransferase [synthetic construct]
Length = 360
Score = 111 bits (278), Expect = 1e-26
Identities = 53/73 (72%), Positives = 61/73 (83%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+M+ WRKKLDKNMEA +EIL PGLG + EAVN VITHIRT C+GGY QFSFN+G
Sbjct: 289 GMHMARSMEEWRKKLDKNMEAAREILEPGLG-SKEAVNQVITHIRTFCLGGYEQFSFNNG 347
Query: 278 DEWMNAHLLFKKK 240
+EWM +LFKKK
Sbjct: 348 EEWMITQMLFKKK 360
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 109 bits (273), Expect = 8e-26
Identities = 52/73 (71%), Positives = 61/73 (83%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+++ W KKLDKNMEA +EIL PGLG + EAVN VITHIRT C+GGY QFSFN+G
Sbjct: 294 GMHMARSVEEWGKKLDKNMEAAREILEPGLG-SKEAVNQVITHIRTFCIGGYEQFSFNNG 352
Query: 278 DEWMNAHLLFKKK 240
+EWM A +LFKKK
Sbjct: 353 EEWMVAQMLFKKK 365
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 106 bits (264), Expect = 1e-24
Identities = 49/73 (67%), Positives = 61/73 (83%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+++AWRKKLD+ +EA +EIL PGLG + EAVN VITHIRT C+GGY QFS+N+G
Sbjct: 279 GMHMARSVEAWRKKLDETIEAAREILEPGLG-SKEAVNQVITHIRTFCIGGYEQFSYNNG 337
Query: 278 DEWMNAHLLFKKK 240
+EWM +LFKKK
Sbjct: 338 EEWMITQILFKKK 350
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 105 bits (263), Expect = 2e-24
Identities = 49/73 (67%), Positives = 60/73 (82%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
GMH AR+++AWRKKLD +EA +EIL PGLG + EAVN VITHIRT C+GGY QFS+N+G
Sbjct: 279 GMHMARSVEAWRKKLDNTIEAAREILEPGLG-SKEAVNQVITHIRTFCIGGYEQFSYNNG 337
Query: 278 DEWMNAHLLFKKK 240
+EWM +LFKKK
Sbjct: 338 EEWMITQILFKKK 350
>ANY58191.1 putative N-methyltransferase [synthetic construct]
Length = 350
Score = 97.8 bits (242), Expect = 2e-21
Identities = 45/73 (61%), Positives = 55/73 (75%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G H AR++ W KKLD NME V+ IL P +GG+ EAV+ VITH RT C+G Y QFSFN+G
Sbjct: 278 GNHMARSVQEWLKKLDDNMETVRGILEPAMGGSKEAVDQVITHSRTFCMGAYEQFSFNNG 337
Query: 278 DEWMNAHLLFKKK 240
+EWM A +LFKKK
Sbjct: 338 EEWMVAQILFKKK 350
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 93.2 bits (230), Expect = 1e-19
Identities = 44/73 (60%), Positives = 55/73 (75%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G H ART++ W KKLDKN++ +EIL P LG + EAV VITH RT C+G QFS+N+G
Sbjct: 286 GKHMARTVEEWLKKLDKNIDVAREILEPSLG-SKEAVEKVITHSRTFCIGTSEQFSYNNG 344
Query: 278 DEWMNAHLLFKKK 240
DEWM +H+LFKKK
Sbjct: 345 DEWMISHVLFKKK 357
>ANY58192.1 putative N-methyltransferase [synthetic construct]
Length = 353
Score = 90.5 bits (223), Expect = 1e-18
Identities = 43/73 (58%), Positives = 51/73 (69%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G H AR+ W K LD NME V+EIL P +GGN EAV+ V+T R C+G Y QFS N+G
Sbjct: 281 GKHMARSAQEWLKSLDDNMEKVREILEPSMGGNKEAVDQVLTQSRFFCMGAYEQFSNNNG 340
Query: 278 DEWMNAHLLFKKK 240
+EWM A LLFKKK
Sbjct: 341 EEWMFAQLLFKKK 353
>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 69.7 bits (169), Expect = 4e-11
Identities = 31/73 (42%), Positives = 47/73 (64%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G HF+RT + W K+LD N++ +K + +G EAV +I + R C+ G F +N+G
Sbjct: 287 GKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVK-LINYWRGFCLSGMEMFGYNNG 345
Query: 278 DEWMNAHLLFKKK 240
+EWM +H+LFKKK
Sbjct: 346 EEWMASHVLFKKK 358
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 69.3 bits (168), Expect = 4e-11
Identities = 34/46 (73%), Positives = 39/46 (84%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRT 321
GMH AR+++ WRKKLDKNMEA +EIL PGL G+ EAVN VITHIRT
Sbjct: 289 GMHMARSVEEWRKKLDKNMEAAREILEPGL-GSKEAVNQVITHIRT 333
>AOR51553.1 putative N-methyltransferase [Papaver somniferum]
Length = 356
Score = 68.9 bits (167), Expect = 7e-11
Identities = 35/73 (47%), Positives = 46/73 (63%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G H AR+ + W K+ N+EA + L GLG + EA N V +T +G VQFSFN+G
Sbjct: 285 GTHMARSQEEWFKRFKINLEAASKALTVGLG-SEEAANQVNIQYKTFFMGYVVQFSFNNG 343
Query: 278 DEWMNAHLLFKKK 240
+EWM +H LFKKK
Sbjct: 344 EEWMISHYLFKKK 356
>Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
AAU20766.1 (S)-coclaurine N-methyltransferase
[Thalictrum flavum subsp. glaucum] ANY58203.1 CNMT
[synthetic construct]
Length = 361
Score = 67.0 bits (162), Expect = 3e-10
Identities = 29/73 (39%), Positives = 45/73 (61%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G HF+RT + W K+LD N+E +K + + G + +I + R C+ G F +N+G
Sbjct: 289 GKHFSRTNEEWLKRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNG 348
Query: 278 DEWMNAHLLFKKK 240
+EWM +H+LFKKK
Sbjct: 349 EEWMASHVLFKKK 361
>ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 66.2 bits (160), Expect = 6e-10
Identities = 34/73 (46%), Positives = 42/73 (57%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G HF RT + W K+LD N+E KEIL G+ EA H R C+ F +N G
Sbjct: 286 GKHFHRTAEEWVKQLDTNLEKGKEIL-ESKYGSKEAALKAFNHWRGLCIFSSEIFGYNGG 344
Query: 278 DEWMNAHLLFKKK 240
+EWM +HLLFKKK
Sbjct: 345 EEWMTSHLLFKKK 357
>AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase, partial
[Papaver bracteatum]
Length = 283
Score = 64.7 bits (156), Expect = 2e-09
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAV 348
GMH AR++D WRK LDKNMEA KEILLPGLGG++EAV
Sbjct: 247 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAV 283
>XP_002870227.1 predicted protein [Arabidopsis lyrata subsp. lyrata] EFH46486.1
predicted protein [Arabidopsis lyrata subsp. lyrata]
Length = 88
Score = 60.5 bits (145), Expect = 3e-09
Identities = 31/73 (42%), Positives = 46/73 (63%)
Frame = -2
Query: 458 GMHFARTMDAWRKKLDKNMEAVKEILLPGLGGNNEAVNGVITHIRTCCVGGYVQFSFNDG 279
G H+A+T + W K++DK + A+KEI+ G EAV ++ + RT + F +N+G
Sbjct: 18 GTHYAKTSEEWLKRMDKEIVAIKEIMEVTYG-KEEAVKWMV-YWRTFFIAVAELFGYNNG 75
Query: 278 DEWMNAHLLFKKK 240
+EWM AH LFKKK
Sbjct: 76 EEWMVAHFLFKKK 88