BLASTX nr result
ID: Papaver32_contig00010070
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00010070
(442 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine N-m... 157 5e-44
ANY58198.1 putative N-methyltransferase [synthetic construct] 154 7e-43
C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 133 7e-35
Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 129 2e-33
ANY58190.1 putative N-methyltransferase [synthetic construct] 127 1e-32
ANY58200.1 putative N-methyltransferase [synthetic construct] 126 2e-32
ANY58201.1 putative N-methyltransferase [synthetic construct] 110 5e-26
ANY58193.1 putative N-methyltransferase [synthetic construct] 109 9e-26
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 107 4e-25
ANY58195.1 TNMT [synthetic construct] 107 6e-25
ANY58191.1 putative N-methyltransferase [synthetic construct] 98 1e-21
ANY58192.1 putative N-methyltransferase [synthetic construct] 92 2e-19
ANY58196.1 putative N-methyltransferase [synthetic construct] 89 2e-18
ANY58202.1 putative N-methyltransferase [synthetic construct] 70 2e-11
AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase,... 69 5e-11
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica] 65 2e-09
AOR51553.1 putative N-methyltransferase [Papaver somniferum] 63 9e-09
Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short... 62 2e-08
ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata] 62 2e-08
AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexan... 59 1e-07
>C3SBU4.1 RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2 ACO90236.1 putative
N-methyltransferase [Papaver bracteatum] ANY58199.1
TNMT2 [synthetic construct]
Length = 358
Score = 157 bits (396), Expect = 5e-44
Identities = 73/73 (100%), Positives = 73/73 (100%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG
Sbjct: 286 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 345
Query: 261 DEWMNAQLLFKKK 223
DEWMNAQLLFKKK
Sbjct: 346 DEWMNAQLLFKKK 358
>ANY58198.1 putative N-methyltransferase [synthetic construct]
Length = 359
Score = 154 bits (388), Expect = 7e-43
Identities = 71/73 (97%), Positives = 73/73 (100%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHI+TCCVGGYVQFSL+DG
Sbjct: 286 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIKTCCVGGYVQFSLSDG 345
Query: 261 DEWMNAQLLFKKK 223
DEWMNAQLLFKKK
Sbjct: 346 DEWMNAQLLFKKK 358
>C3SBU5.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 ACO90237.1 tetrahydroprotoberberine
N-methyltransferase [Papaver bracteatum]
Length = 358
Score = 133 bits (334), Expect = 7e-35
Identities = 61/73 (83%), Positives = 66/73 (90%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+VD WRK LDKNMEA KEILLPGLGG+HEAVNGV+THIRT C+GGY QFS+NDG
Sbjct: 286 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDG 345
Query: 261 DEWMNAQLLFKKK 223
DEWM AQLLFKKK
Sbjct: 346 DEWMVAQLLFKKK 358
>Q108P1.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT AAY79177.1
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 129 bits (325), Expect = 2e-33
Identities = 59/73 (80%), Positives = 65/73 (89%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+VD WRK LDKNMEA KEILLPGLGG+HE VNGV+THIRT C+GGY QFS+N+G
Sbjct: 286 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNG 345
Query: 261 DEWMNAQLLFKKK 223
DEWM AQLLFKKK
Sbjct: 346 DEWMVAQLLFKKK 358
>ANY58190.1 putative N-methyltransferase [synthetic construct]
Length = 362
Score = 127 bits (319), Expect = 1e-32
Identities = 57/73 (78%), Positives = 66/73 (90%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+V+AWRKKLDKNM+A KEILLPGLGGNHEAVN ++THIRT C+GGY QFS N+G
Sbjct: 290 GMHMARSVEAWRKKLDKNMKAAKEILLPGLGGNHEAVNQIVTHIRTFCMGGYEQFSYNNG 349
Query: 261 DEWMNAQLLFKKK 223
+EWM AQ+LFKKK
Sbjct: 350 EEWMVAQMLFKKK 362
>ANY58200.1 putative N-methyltransferase [synthetic construct]
Length = 358
Score = 126 bits (317), Expect = 2e-32
Identities = 58/73 (79%), Positives = 64/73 (87%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+ DAWRKK+DKNME KEILLPGLGG+HEAVN V+THIRT C+GGY QFSLN+G
Sbjct: 286 GMHTARSFDAWRKKVDKNMEVAKEILLPGLGGSHEAVNAVVTHIRTFCMGGYEQFSLNNG 345
Query: 261 DEWMNAQLLFKKK 223
DEWM A LLFKKK
Sbjct: 346 DEWMVAHLLFKKK 358
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 110 bits (274), Expect = 5e-26
Identities = 53/73 (72%), Positives = 60/73 (82%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+V+ W KKLDKNMEA +EIL PGLG EAVN VITHIRT C+GGY QFS N+G
Sbjct: 294 GMHMARSVEEWGKKLDKNMEAAREILEPGLGSK-EAVNQVITHIRTFCIGGYEQFSFNNG 352
Query: 261 DEWMNAQLLFKKK 223
+EWM AQ+LFKKK
Sbjct: 353 EEWMVAQMLFKKK 365
>ANY58193.1 putative N-methyltransferase [synthetic construct]
Length = 360
Score = 109 bits (272), Expect = 9e-26
Identities = 52/73 (71%), Positives = 60/73 (82%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+++ WRKKLDKNMEA +EIL PGLG EAVN VITHIRT C+GGY QFS N+G
Sbjct: 289 GMHMARSMEEWRKKLDKNMEAAREILEPGLGSK-EAVNQVITHIRTFCLGGYEQFSFNNG 347
Query: 261 DEWMNAQLLFKKK 223
+EWM Q+LFKKK
Sbjct: 348 EEWMITQMLFKKK 360
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 107 bits (267), Expect = 4e-25
Identities = 51/73 (69%), Positives = 60/73 (82%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+V+AWRKKLD+ +EA +EIL PGLG EAVN VITHIRT C+GGY QFS N+G
Sbjct: 279 GMHMARSVEAWRKKLDETIEAAREILEPGLGSK-EAVNQVITHIRTFCIGGYEQFSYNNG 337
Query: 261 DEWMNAQLLFKKK 223
+EWM Q+LFKKK
Sbjct: 338 EEWMITQILFKKK 350
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 107 bits (266), Expect = 6e-25
Identities = 51/73 (69%), Positives = 59/73 (80%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
GMH AR+V+AWRKKLD +EA +EIL PGLG EAVN VITHIRT C+GGY QFS N+G
Sbjct: 279 GMHMARSVEAWRKKLDNTIEAAREILEPGLGSK-EAVNQVITHIRTFCIGGYEQFSYNNG 337
Query: 261 DEWMNAQLLFKKK 223
+EWM Q+LFKKK
Sbjct: 338 EEWMITQILFKKK 350
>ANY58191.1 putative N-methyltransferase [synthetic construct]
Length = 350
Score = 98.2 bits (243), Expect = 1e-21
Identities = 46/73 (63%), Positives = 55/73 (75%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G H AR+V W KKLD NME V+ IL P +GG+ EAV+ VITH RT C+G Y QFS N+G
Sbjct: 278 GNHMARSVQEWLKKLDDNMETVRGILEPAMGGSKEAVDQVITHSRTFCMGAYEQFSFNNG 337
Query: 261 DEWMNAQLLFKKK 223
+EWM AQ+LFKKK
Sbjct: 338 EEWMVAQILFKKK 350
>ANY58192.1 putative N-methyltransferase [synthetic construct]
Length = 353
Score = 92.4 bits (228), Expect = 2e-19
Identities = 44/73 (60%), Positives = 52/73 (71%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G H AR+ W K LD NME V+EIL P +GGN EAV+ V+T R C+G Y QFS N+G
Sbjct: 281 GKHMARSAQEWLKSLDDNMEKVREILEPSMGGNKEAVDQVLTQSRFFCMGAYEQFSNNNG 340
Query: 261 DEWMNAQLLFKKK 223
+EWM AQLLFKKK
Sbjct: 341 EEWMFAQLLFKKK 353
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 89.4 bits (220), Expect = 2e-18
Identities = 44/73 (60%), Positives = 52/73 (71%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G H ARTV+ W KKLDKN++ +EIL P LG EAV VITH RT C+G QFS N+G
Sbjct: 286 GKHMARTVEEWLKKLDKNIDVAREILEPSLGSK-EAVEKVITHSRTFCIGTSEQFSYNNG 344
Query: 261 DEWMNAQLLFKKK 223
DEWM + +LFKKK
Sbjct: 345 DEWMISHVLFKKK 357
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 70.1 bits (170), Expect = 2e-11
Identities = 35/46 (76%), Positives = 39/46 (84%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRT 304
GMH AR+V+ WRKKLDKNMEA +EIL PGL G+ EAVN VITHIRT
Sbjct: 289 GMHMARSVEEWRKKLDKNMEAAREILEPGL-GSKEAVNQVITHIRT 333
>AKN62838.1 (S)-tetrahydroprotoberberine-cis-N-methyltransferase, partial
[Papaver bracteatum]
Length = 283
Score = 68.6 bits (166), Expect = 5e-11
Identities = 31/37 (83%), Positives = 33/37 (89%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAV 331
GMH AR+VD WRK LDKNMEA KEILLPGLGG+HEAV
Sbjct: 247 GMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAV 283
>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 64.7 bits (156), Expect = 2e-09
Identities = 30/73 (41%), Positives = 45/73 (61%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G HF+RT + W K+LD N++ +K + +G EAV +I + R C+ G F N+G
Sbjct: 287 GKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVK-LINYWRGFCLSGMEMFGYNNG 345
Query: 261 DEWMNAQLLFKKK 223
+EWM + +LFKKK
Sbjct: 346 EEWMASHVLFKKK 358
>AOR51553.1 putative N-methyltransferase [Papaver somniferum]
Length = 356
Score = 62.8 bits (151), Expect = 9e-09
Identities = 33/73 (45%), Positives = 44/73 (60%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G H AR+ + W K+ N+EA + L GLG + EA N V +T +G VQFS N+G
Sbjct: 285 GTHMARSQEEWFKRFKINLEAASKALTVGLG-SEEAANQVNIQYKTFFMGYVVQFSFNNG 343
Query: 261 DEWMNAQLLFKKK 223
+EWM + LFKKK
Sbjct: 344 EEWMISHYLFKKK 356
>Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
AAU20766.1 (S)-coclaurine N-methyltransferase
[Thalictrum flavum subsp. glaucum] ANY58203.1 CNMT
[synthetic construct]
Length = 361
Score = 62.0 bits (149), Expect = 2e-08
Identities = 28/73 (38%), Positives = 43/73 (58%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G HF+RT + W K+LD N+E +K + + G + +I + R C+ G F N+G
Sbjct: 289 GKHFSRTNEEWLKRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNG 348
Query: 261 DEWMNAQLLFKKK 223
+EWM + +LFKKK
Sbjct: 349 EEWMASHVLFKKK 361
>ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 61.6 bits (148), Expect = 2e-08
Identities = 33/73 (45%), Positives = 40/73 (54%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G HF RT + W K+LD N+E KEIL G+ EA H R C+ F N G
Sbjct: 286 GKHFHRTAEEWVKQLDTNLEKGKEIL-ESKYGSKEAALKAFNHWRGLCIFSSEIFGYNGG 344
Query: 261 DEWMNAQLLFKKK 223
+EWM + LLFKKK
Sbjct: 345 EEWMTSHLLFKKK 357
>AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
Length = 370
Score = 59.3 bits (142), Expect = 1e-07
Identities = 28/73 (38%), Positives = 41/73 (56%)
Frame = -2
Query: 441 GMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDG 262
G HF+RT + W K+LD N++ +K I LG AV +I + + C G F N+G
Sbjct: 299 GKHFSRTNEEWLKRLDANVDTIKAIFEASLGSEEAAVK-LINYWKGFCFSGMEMFGSNNG 357
Query: 261 DEWMNAQLLFKKK 223
+EWM + LL +K
Sbjct: 358 EEWMTSHLLLTRK 370