BLASTX nr result
ID: Papaver32_contig00006546
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00006546
(1312 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 647 0.0
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 644 0.0
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 643 0.0
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik... 616 0.0
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 596 0.0
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 595 0.0
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 592 0.0
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 539 0.0
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 533 0.0
AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum fla... 524 0.0
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 370 e-121
XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 363 e-118
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 361 e-117
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 361 e-117
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 360 e-117
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 357 e-115
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 356 e-115
XP_009610421.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 352 e-114
XP_016435506.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 350 e-113
AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum] 350 e-113
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 647 bits (1670), Expect = 0.0
Identities = 316/375 (84%), Positives = 347/375 (92%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPHSQFAQLA+TYGD+F+LKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 30 WPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSG 89
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCRTELF+QKMI+SQAE RE KAMEMVEY
Sbjct: 90 RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEY 149
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
LKKN GNEVKI EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 150 LKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 209
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFPFLG DL GQ K+VADCLQGIY VWG +LKER+IA KQ N KKNDFV++LLD+
Sbjct: 210 PADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIA-KQHNNSKKNDFVEILLDS 268
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D+QINALLMEIFG GTETS STIEWA +ELTKNPQV ANMR ELLSVVGKRP+KESD
Sbjct: 269 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESD 328
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
IPNMPYLQAFVKETLRLHP TPLL+PRRALETC+VLN+TIPKECQIMVNAWGIGRDPK W
Sbjct: 329 IPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRW 388
Query: 1268 TDPLKFSPERFLNSS 1312
TDPLKFSPERFLNSS
Sbjct: 389 TDPLKFSPERFLNSS 403
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 644 bits (1661), Expect = 0.0
Identities = 314/375 (83%), Positives = 346/375 (92%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPHSQFAQLA+TYGD+F+LKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 30 WPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSG 89
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCRT+LF+QKMI+SQAE RE KAMEMVEY
Sbjct: 90 RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAMEMVEY 149
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
LKKN GNEVKI EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 150 LKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 209
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFPFLG DL GQ K+VADCLQGIY VWG +LKE +IA KQ N KKNDFV++LLD+
Sbjct: 210 PADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIA-KQHNNSKKNDFVEILLDS 268
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D+QINALLMEIFG GTETS STIEWA +ELTKNPQV ANMR ELLSVVGKRP+KESD
Sbjct: 269 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESD 328
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
IPNMPYLQAFVKETLRLHP TPLL+PRRALETC+VLN+TIPKECQIMVNAWGIGRDPK W
Sbjct: 329 IPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRW 388
Query: 1268 TDPLKFSPERFLNSS 1312
TDPLKFSPERFLNSS
Sbjct: 389 TDPLKFSPERFLNSS 403
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 643 bits (1659), Expect = 0.0
Identities = 311/375 (82%), Positives = 349/375 (93%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPHSQFA+LAKTYGD+FTLKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 35 WPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSG 94
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCR ELF+QKMI+SQAE RE KAMEMVE+
Sbjct: 95 RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEF 154
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
LK+N+G+EVKI+EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 155 LKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 214
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFPFLG DL GQRK+VADCLQGIY VWG +LKER+IA K N KKNDFV++LLD+
Sbjct: 215 PADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIA-KLHNNSKKNDFVEILLDS 273
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D+QINALLMEIFG GTETS STIEWA +ELTKNP+V ANMR+ELLSVVGKRP+KESD
Sbjct: 274 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESD 333
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
IPNMPYLQAFVKETLRLHP TPLL+PRRALETC+VLN+TIPKECQIMVNAWGIGRDPK W
Sbjct: 334 IPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRW 393
Query: 1268 TDPLKFSPERFLNSS 1312
TDPLKF+PERFLNSS
Sbjct: 394 TDPLKFAPERFLNSS 408
>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
P450 [Papaver somniferum]
Length = 440
Score = 616 bits (1589), Expect = 0.0
Identities = 295/375 (78%), Positives = 339/375 (90%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPHSQFAQLA+TYGD+FTLKLGT+TVVVASTPLAASE+LK HDR L G
Sbjct: 36 WPIVGNLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCG 95
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+ HV+ S+ W++CNETW KLRKVCRT+LF+QKMI++QAE RE K MEMV+Y
Sbjct: 96 RYVFQSFRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKY 155
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
LKKNEG EVKI+EVIFGTLVN+FGNLIFSQ+IF+LGDE+SGS++MK+ IWRMLELGN+ N
Sbjct: 156 LKKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSAN 215
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
P DY P LGSLDL GQRK+VADCLQG+Y VWG +LKERRIA +Q KNDFVDVLLD+
Sbjct: 216 PGDYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDS 275
Query: 911 GLGDRQINALLMEIF-GGTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D+QIN+LL+++F GTET+ STIEWA ELTKNPQV A++R+ELLSVVGKR +KESD
Sbjct: 276 GLDDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESD 335
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
I N+PYLQAFVKETLRLHPPTPLL+PRRALETCQVLN+TIPKECQIMVNAWGIGRDPK W
Sbjct: 336 ILNLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTW 395
Query: 1268 TDPLKFSPERFLNSS 1312
TDPLKFSP+RFLNSS
Sbjct: 396 TDPLKFSPDRFLNSS 410
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 596 bits (1536), Expect = 0.0
Identities = 287/375 (76%), Positives = 326/375 (86%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST AASEILKTHDRILS
Sbjct: 35 WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 94
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+GHV+ S+ WSDC ETW LRKVCRTELF+QKMI+SQA RE K EMVEY
Sbjct: 95 RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 154
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELGD NSGS + KE +WRMLELGN+TN
Sbjct: 155 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTN 214
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFP LG DL GQRKEVA+CL+GIY +WG +L+ER++A K D KNDFVDV LD+
Sbjct: 215 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDS 274
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D QINALLME+FG GTETS STIEWA ELTKNP++ A +R+E+ +VVG+R +KESD
Sbjct: 275 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESD 334
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
PN+PYL+A VKETLRLHPPTPLL+PRRALETC +LN+TIPK+CQIMVNAWGIGRDPK W
Sbjct: 335 FPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTW 394
Query: 1268 TDPLKFSPERFLNSS 1312
TDPL FSPERFLNSS
Sbjct: 395 TDPLTFSPERFLNSS 409
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 595 bits (1533), Expect = 0.0
Identities = 287/375 (76%), Positives = 325/375 (86%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST AASEILKTHDRILS
Sbjct: 36 WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 95
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+GHV+ S+ WSDC ETW LRKVCRTELF+QKMI+SQA RE K EMVEY
Sbjct: 96 RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 155
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELGD NSGS + KE +WRMLELGN+TN
Sbjct: 156 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTN 215
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFP LG DL GQRKEVA+CL+GIY +WG +L+ER++A K D KNDFVDV LD+
Sbjct: 216 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDS 275
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D QINALLME+FG GTETS STIEWA ELTKNP++ A +R+EL +VVG+R +KESD
Sbjct: 276 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESD 335
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
PN+PYL+A VKETLRLHPPTPLL+PRRALETC +LN+TIPK+CQIMVNAWGIGRDPK W
Sbjct: 336 FPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTW 395
Query: 1268 TDPLKFSPERFLNSS 1312
DPL FSPERFLNSS
Sbjct: 396 IDPLTFSPERFLNSS 410
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 592 bits (1526), Expect = 0.0
Identities = 286/375 (76%), Positives = 324/375 (86%), Gaps = 1/375 (0%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST AASEILKTHDRILS
Sbjct: 36 WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 95
Query: 371 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 550
RYV+QSFRV+GHV+ S+ WSDC ETW LRKVCRTELF+QKMI+SQA RE K EMVEY
Sbjct: 96 RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 155
Query: 551 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 730
L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELG NSGS + KE +WRMLELGN+TN
Sbjct: 156 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTN 215
Query: 731 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 910
PADYFP LG DL GQRKEVA+CL+GIY +WG +L+ER++A K D KNDFVDV LD+
Sbjct: 216 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDS 275
Query: 911 GLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESD 1087
GL D QINALLME+FG GTETS STIEWA ELTKNP++ A +R+EL +VVG+R +KESD
Sbjct: 276 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESD 335
Query: 1088 IPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNW 1267
PN+PYL+A VKETLRLHPPTPLL+PRRALETC +LN+TIPK+CQIMVNAWGIGRDPK W
Sbjct: 336 FPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTW 395
Query: 1268 TDPLKFSPERFLNSS 1312
DPL FSPERFLNSS
Sbjct: 396 IDPLTFSPERFLNSS 410
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 539 bits (1388), Expect = 0.0
Identities = 257/373 (68%), Positives = 316/373 (84%), Gaps = 1/373 (0%)
Frame = +2
Query: 194 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 373
PIVGNLLQL DKPH++FA+LA+ YG++F+LKLG++TVVVAS+P AA+EILKTHD+ILSGR
Sbjct: 37 PIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGR 96
Query: 374 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 553
YV+QSFRV+ HV+ S+ WS+CN+ W LRKVCRTELF+ KMI+SQ+E RE KA EMV++L
Sbjct: 97 YVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFL 156
Query: 554 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 733
+ EG VKI+EV+FGTLVNIFGNLIFS+++F+L D GSV++KE +W++L++GN+TNP
Sbjct: 157 RGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNP 216
Query: 734 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 913
ADYFP +G LDL GQR+ VA+ LQ IYDVWGV+LKERR + KNDFVDVLL+ G
Sbjct: 217 ADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSE---SKNDFVDVLLNAG 273
Query: 914 LGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESDI 1090
L D++INALLME+FG GTETS STIEWA ELTK P V + +R EL++VVG +KESD+
Sbjct: 274 LDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDL 333
Query: 1091 PNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNWT 1270
P++PYLQAFVKETLRLHPPTPLL+PRRALETC V+N+TIPKECQIMVNAW IGRDPK W
Sbjct: 334 PHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWD 393
Query: 1271 DPLKFSPERFLNS 1309
DPL F PERFL+S
Sbjct: 394 DPLNFKPERFLSS 406
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 533 bits (1372), Expect = 0.0
Identities = 255/373 (68%), Positives = 312/373 (83%), Gaps = 1/373 (0%)
Frame = +2
Query: 194 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 373
PIVGNLLQL DKP ++FA+LA+ YG +F+LKLG++TVVVAS+P AA+E+LKTHD+ILSGR
Sbjct: 37 PIVGNLLQLGDKPRAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGR 96
Query: 374 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 553
YV+QSFRV+ HV+ S+ WS+CN+ W LRKVCRTELF+ KMI+SQ+E RE KA EMV++L
Sbjct: 97 YVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFL 156
Query: 554 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 733
+ EG VKI+EV+FGTLVNIFGNLIFS+++F+L D GSV++KE +W++LE+GN+TNP
Sbjct: 157 RSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNP 216
Query: 734 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 913
ADYFP +G DL GQR+ VAD LQ IYDVWG+ LKERR + N NDFVDVLL+ G
Sbjct: 217 ADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESN---NDFVDVLLNAG 273
Query: 914 LGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESDI 1090
L D++INALLME+FG GTETS STIEWA ELTK P V + +R EL++VVG +KESD+
Sbjct: 274 LEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDL 333
Query: 1091 PNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNWT 1270
P++PYLQAFVKETLRLHPPTPLL+PRRALETC V+N+TIPKECQIMVNAW IGRDPK W
Sbjct: 334 PHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWD 393
Query: 1271 DPLKFSPERFLNS 1309
DPL F PERFL+S
Sbjct: 394 DPLNFKPERFLSS 406
>AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 524 bits (1350), Expect = 0.0
Identities = 250/374 (66%), Positives = 312/374 (83%), Gaps = 1/374 (0%)
Frame = +2
Query: 194 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 373
PIVGNLLQL +KPH++FA+LA+ YG++FTLKLG++TVVVAS+P AA+EILKT D+ILSGR
Sbjct: 38 PIVGNLLQLGEKPHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGR 97
Query: 374 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 553
YV+QSFRV HV S+ WS+CNE W LRKVCRTELFS KMI+SQA RE KA++MV +L
Sbjct: 98 YVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFL 157
Query: 554 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 733
+K E EVKI+EV+F TLVNIFGNLIFS+++F+L D GS ++KE +W++L++GN+TNP
Sbjct: 158 RKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNP 217
Query: 734 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 913
ADYFP +G DL GQR+EVA L+ IYDVWGV+LKERR ++NDF +VLL+ G
Sbjct: 218 ADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGH----RENDFANVLLNAG 273
Query: 914 LGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIKESDI 1090
L D++INALLME+FG GTET+ STIEWA ELT N +V + +RAEL++VVG + +KES+I
Sbjct: 274 LDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVGHKTVKESNI 333
Query: 1091 PNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPKNWT 1270
P++PYLQA VKETLRLHPPTPLL+PRRALETC+V+N+TIPKECQIMVNAW IGRDPK W
Sbjct: 334 PHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWD 393
Query: 1271 DPLKFSPERFLNSS 1312
DPL F PERF+NS+
Sbjct: 394 DPLTFKPERFMNST 407
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 370 bits (951), Expect = e-121
Identities = 186/376 (49%), Positives = 260/376 (69%), Gaps = 3/376 (0%)
Frame = +2
Query: 191 WPIVGNLL-QLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILS 367
WPI+G LL +L +PH + ++LA+ +G + LK G E VVVAS+ +AA E+LK DR+LS
Sbjct: 35 WPIIGTLLPKLKKQPHVELSKLAQRFGPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLS 94
Query: 368 GRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVE 547
GR+ S R++G+++ SM W+DC + W +RKV RTELFS KM+ QA ARE K E+++
Sbjct: 95 GRFAPHSVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMK 154
Query: 548 YLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTT 727
+L + EG +V +VIFG+++NI G LIFS+++++ D ++ MK I +++ L
Sbjct: 155 FLIRKEGEKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIP 214
Query: 728 NPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLD 907
N AD +P LG D G RK A C++ + + W ++K+RR K D + KNDF+ VLLD
Sbjct: 215 NLADLYPILGGSDFQGLRKASAACVKRMNESWAAIVKQRR---KNDDH-SKNDFLQVLLD 270
Query: 908 TGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGK-RPIKE 1081
+G D QI+A+L+E FG G++TS STIEWA AEL +NP+ +R EL V+ + +KE
Sbjct: 271 SGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKE 330
Query: 1082 SDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDPK 1261
SD+PN+PYL A VKETLRLHPP L+P RA+ETCQ++N+TIPK CQ+MVN + IGRD K
Sbjct: 331 SDLPNLPYLHACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSK 390
Query: 1262 NWTDPLKFSPERFLNS 1309
W PL F PERFLNS
Sbjct: 391 TWEKPLSFLPERFLNS 406
>XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus
communis] EEF51432.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 363 bits (932), Expect = e-118
Identities = 174/378 (46%), Positives = 249/378 (65%), Gaps = 4/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GN+LQ+ K H A AK YG + +L+LG + +VVASTP +A+E+LK HDR+LS
Sbjct: 43 WPILGNILQVDKKLHICIANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSA 102
Query: 371 RYVYQSFRVEGHV--KVSMAWSD-CNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 541
R++ ++ E H+ +V++ W+ CN+ W LR +CRTELFS K I+SQA RE K EM
Sbjct: 103 RFILKAIPSESHILERVAIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEM 162
Query: 542 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 721
+E+L +G V + EV+FGT+ N +L+FS ++ ++ G +K +W M+E+
Sbjct: 163 LEFLITKQGQAVNVAEVVFGTIFNTISHLLFSTDLIGFENQIGG---VKSLLWSMMEMAT 219
Query: 722 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 901
+ N A+++P L LD G ++++ CL+ ++ VW + +KERR + D K DF+D+
Sbjct: 220 SPNIAEFYPILAPLDPQGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIF 279
Query: 902 LDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIK 1078
L G D QIN L+ME+ GT+T+ +++EWA AEL KN + +R EL + K PIK
Sbjct: 280 LSNGFDDDQINWLVMELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIK 339
Query: 1079 ESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDP 1258
ES + +PYL A VKETLRLHPP P L+PRRA E C+V+N+TIPK+ Q++VN W IGRDP
Sbjct: 340 ESHVSQLPYLNACVKETLRLHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDP 399
Query: 1259 KNWTDPLKFSPERFLNSS 1312
W DP F PERFL SS
Sbjct: 400 SVWEDPSSFKPERFLGSS 417
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 361 bits (927), Expect = e-117
Identities = 185/378 (48%), Positives = 253/378 (66%), Gaps = 5/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQL---SDKP-HSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDR 358
WP++GNL L ++ P H A +A+T+G I L LGT+ V+AST AA EILKTHDR
Sbjct: 35 WPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDR 94
Query: 359 ILSGRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 538
I S R++ SFR++ H+K S+ WSDC + W LRK+ RTE+FS KM+Q+Q+ RE K E
Sbjct: 95 IFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAE 154
Query: 539 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 718
++++L+ EG VKI + +FGTL+NI GN++FS+++F GDE ++ I ML +G
Sbjct: 155 LIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKG-GIQNLIREMLMIG 213
Query: 719 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 898
N A+++P L LDL G +K+ + + +W +KER K ++N + D +DV
Sbjct: 214 AEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKER----KANRNEESKDMLDV 269
Query: 899 LLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPI 1075
LL D QINAL +E FG G+ETS +TIEW AEL K+P+ A +R EL VVG I
Sbjct: 270 LLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTI 329
Query: 1076 KESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRD 1255
KESD+P +PYLQA +KE +RLHP P L+PRRA ETC+V+ +TIPK Q++VNA+ IGRD
Sbjct: 330 KESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRD 389
Query: 1256 PKNWTDPLKFSPERFLNS 1309
PK+W DP F PERFL S
Sbjct: 390 PKSWKDPSTFWPERFLES 407
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 361 bits (926), Expect = e-117
Identities = 179/378 (47%), Positives = 244/378 (64%), Gaps = 5/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GNLLQ+ PH++ A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45 WPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSG 104
Query: 371 RYVYQSFRVEG----HVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 538
RY+ S V+ H+ + A DCN W LR +CR ELFS K ++SQ E RE K E
Sbjct: 105 RYLSSSVPVKNPKLNHLSIVFA-KDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTE 163
Query: 539 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 718
+VE+L EG VK+++++F T+ NI N FS ++ + DE +K+ I + E G
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFG 223
Query: 719 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 898
T N +DY+P LG LD+ G ++ + + I W +LKERR ++ DF++
Sbjct: 224 ATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEA 281
Query: 899 LLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPI 1075
LL+ G D QIN +++E+F G ETS T+EWA AEL +N +R EL +VG+ P+
Sbjct: 282 LLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPV 341
Query: 1076 KESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRD 1255
+ES +P +PYLQA VKE LRLHPP PLL+P A ETCQV+ +TIPK+ QI VN W + RD
Sbjct: 342 RESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARD 401
Query: 1256 PKNWTDPLKFSPERFLNS 1309
PK W DPL F PERFL+S
Sbjct: 402 PKIWDDPLSFKPERFLDS 419
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 360 bits (925), Expect = e-117
Identities = 179/378 (47%), Positives = 244/378 (64%), Gaps = 5/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GNLLQ+ PH++ A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45 WPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSG 104
Query: 371 RYVYQSFRVEG----HVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 538
RY+ S V+ H+ + A DCN W LR +CR ELFS K ++SQ E RE K E
Sbjct: 105 RYLSXSVPVKNPKLNHLSIVFA-KDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTE 163
Query: 539 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 718
+VE+L EG VK+++++F T+ NI N FS ++ + DE +K+ I + E G
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFG 223
Query: 719 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 898
T N +DY+P LG LD+ G ++ + + I W +LKERR ++ DF++
Sbjct: 224 ATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEA 281
Query: 899 LLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPI 1075
LL+ G D QIN +++E+F G ETS T+EWA AEL +N +R EL +VG+ P+
Sbjct: 282 LLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPV 341
Query: 1076 KESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRD 1255
+ES +P +PYLQA VKE LRLHPP PLL+P A ETCQV+ +TIPK+ QI VN W + RD
Sbjct: 342 RESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARD 401
Query: 1256 PKNWTDPLKFSPERFLNS 1309
PK W DPL F PERFL+S
Sbjct: 402 PKIWDDPLSFKPERFLDS 419
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 357 bits (916), Expect = e-115
Identities = 178/378 (47%), Positives = 249/378 (65%), Gaps = 5/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GNLLQL PH + A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45 WPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSG 104
Query: 371 RYVYQSFRVE----GHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 538
RYV S V+ H+ ++ A +C W LR +CRTE+FS K ++S E RE K ME
Sbjct: 105 RYVSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVME 163
Query: 539 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 718
+VE+L EG VK+++++F T+ NI N FS ++ + E +K+ I+R ELG
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELG 218
Query: 719 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 898
T N +D++P L L+L G +K+ + L I W LKERR +++ D ++
Sbjct: 219 ATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEA 276
Query: 899 LLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPI 1075
L+ D QIN +++E+F G +TS TIEWA +L +NP V +R EL ++G+ P+
Sbjct: 277 FLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPV 336
Query: 1076 KESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRD 1255
+ES +P++PYLQA VKETLRLHPP PLL+P RA+ETCQV+ +TIPK+ Q+ VN W +GRD
Sbjct: 337 RESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRD 396
Query: 1256 PKNWTDPLKFSPERFLNS 1309
PK W DPL F+PERFL+S
Sbjct: 397 PKVWDDPLSFTPERFLDS 414
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 356 bits (913), Expect = e-115
Identities = 179/378 (47%), Positives = 252/378 (66%), Gaps = 4/378 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GN+LQ+ PH A AK +G + +L+LGT VVVAS+P AA+EILKTHDR+LSG
Sbjct: 44 WPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSG 103
Query: 371 RYVYQSFRVEGHV--KVSMAWS-DCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 541
RY+ + E +V ++++ W+ C++ W LR +CR+ELFS K I+SQA RE K EM
Sbjct: 104 RYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEM 163
Query: 542 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 721
+++L +G V I EV+F T N NL+FS+++ D+ + ++K I ++EL
Sbjct: 164 LDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLISTLMELAT 222
Query: 722 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 901
N AD++P L LD G ++++ +CL+ ++ VW + +KERR + K+ +K DF+DV
Sbjct: 223 CPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERR--ERHVKDARKTDFLDVF 280
Query: 902 LDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIK 1078
L G D QIN LL+E+F G +T+ +T+EWA AEL K V +R EL + +GK I+
Sbjct: 281 LSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIR 340
Query: 1079 ESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDP 1258
ES IP + YL A VKETLRLHPP P L+PRRA E C+V+N+TIPK QI+VN W IGRDP
Sbjct: 341 ESHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDP 400
Query: 1259 KNWTDPLKFSPERFLNSS 1312
W DPL + PERFL+S+
Sbjct: 401 SAWEDPLSYRPERFLDSN 418
>XP_009610421.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Nicotiana tomentosiformis]
Length = 489
Score = 352 bits (903), Expect = e-114
Identities = 184/382 (48%), Positives = 252/382 (65%), Gaps = 8/382 (2%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GN+LQL PH Q AK +G + L+LGT+ VV+AS+P +A+EILKT DR+LS
Sbjct: 37 WPILGNILQLGKNPHISMTQFAKIHGPLICLRLGTQLVVIASSPDSAAEILKTQDRLLSA 96
Query: 371 RYV-----YQSFRVEGHVKVSMAWS-DCNETWHKLRKVCRTELFSQKMIQSQAEARECKA 532
R V Y+ ++ H S+ WS D W LR CRT LFS K ++SQA+ RE K
Sbjct: 97 RSVPRVAPYKLSVIDQH---SIVWSSDLTNHWKSLRAFCRTHLFSAKAVESQADLREKKV 153
Query: 533 MEMVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLE 712
EM+E+L+ N+G VKI E++F T++N GNL F++N+ +L E + S +K I R +E
Sbjct: 154 FEMIEFLRSNKGKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNTS-GIKHVIRRFVE 212
Query: 713 LGNTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFV 892
LG N ++++PFL +LDL G RK + +W V+KE+R A + K DF+
Sbjct: 213 LGAMPNISEFYPFLDALDLQGLRKLTNIYQDQLISIWAEVVKEKRQAISCGSSNKNQDFL 272
Query: 893 DVLLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKR 1069
D+++D+G D QIN +LME+ GT+T+ STIEWA AEL +N +V ++AE+ S +G
Sbjct: 273 DIMIDSGFSDLQINIILMELIAAGTDTTTSTIEWAMAELLRNKKVMHKLQAEIRSKIGSN 332
Query: 1070 P-IKESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGI 1246
I+ES+I +PYL A VKETLR+HPPTP LVPRRA ETC+++NFTIPK ++ VN W I
Sbjct: 333 ANIRESNISELPYLAACVKETLRIHPPTPFLVPRRAPETCKLMNFTIPKNSRLFVNVWAI 392
Query: 1247 GRDPKNWTDPLKFSPERFLNSS 1312
GRD K W D L F PERFL+SS
Sbjct: 393 GRDSKTWEDALSFRPERFLDSS 414
>XP_016435506.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Nicotiana tabacum]
Length = 446
Score = 350 bits (897), Expect = e-113
Identities = 182/382 (47%), Positives = 252/382 (65%), Gaps = 8/382 (2%)
Frame = +2
Query: 191 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 370
WPI+GN+LQL PH Q AK +G + L+LGT+ VV+AS+P +A+EILKT DR+LS
Sbjct: 37 WPILGNILQLGKNPHISMTQFAKIHGPLICLRLGTQLVVIASSPDSAAEILKTQDRLLSA 96
Query: 371 RYV-----YQSFRVEGHVKVSMAWS-DCNETWHKLRKVCRTELFSQKMIQSQAEARECKA 532
R V Y+ ++ H S+ WS D W LR CRT LFS K ++SQA+ RE K
Sbjct: 97 RSVPRVAPYKLSVIDQH---SIVWSSDLTNHWKSLRAFCRTHLFSAKAVESQADLREKKV 153
Query: 533 MEMVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLE 712
EM+E+L+ N+G VKI E++F T++N GNL F++N+ +L E + S +K I + +E
Sbjct: 154 FEMIEFLRSNKGKTVKISEILFATILNTLGNLFFTKNLCDLDSEGNTS-GIKHVIRKFVE 212
Query: 713 LGNTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFV 892
LG N ++++PFL +LDL G RK + +W V+KE+R A + K DF+
Sbjct: 213 LGAMPNISEFYPFLDALDLQGLRKLTNIYQDQLISIWAEVVKEKRQAISCGSSNKNQDFL 272
Query: 893 DVLLDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKR 1069
D+++D+G D QIN +LME+ GT+T+ STIEWA AEL ++ +V ++AE+ S +G
Sbjct: 273 DIMIDSGFSDLQINIILMELIAAGTDTTTSTIEWAMAELLRSKKVMHKLQAEIRSTIGSN 332
Query: 1070 P-IKESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGI 1246
I+ES+I +PYL A VKETLR+HPPTP LVPRRA ETC+++NFTIPK ++ VN W I
Sbjct: 333 ANIRESNISELPYLAACVKETLRIHPPTPFLVPRRAPETCKLMNFTIPKNSRLFVNVWAI 392
Query: 1247 GRDPKNWTDPLKFSPERFLNSS 1312
GRD K W D L F PERFL+SS
Sbjct: 393 GRDSKTWEDALSFRPERFLDSS 414
>AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 350 bits (899), Expect = e-113
Identities = 180/377 (47%), Positives = 242/377 (64%), Gaps = 4/377 (1%)
Frame = +2
Query: 191 WPIVGNLLQLSDKP---HSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRI 361
WPI+GN+ L H +A+T+G I L LGT+ VAST AA EILKTHDR
Sbjct: 34 WPIIGNIPALFSSAYPMHVALTNMARTHGPIMILWLGTQPTAVASTTEAAMEILKTHDRS 93
Query: 362 LSGRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 541
SGR V SFR++ H+K S+ W+DC+E W LRK+ RTE+FS KM+Q Q RE K E+
Sbjct: 94 FSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAEL 153
Query: 542 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 721
VE+LK EG VKI +++FGTL+NI GN+IFS+++F + M+ I ML +G
Sbjct: 154 VEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGDK--MQTLIKEMLMIGA 211
Query: 722 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 901
N A+++P DL G +K + +W ++ER++ +N + D +DVL
Sbjct: 212 APNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVK----RNEEPKDMLDVL 267
Query: 902 LDTGLGDRQINALLMEIFG-GTETSGSTIEWAFAELTKNPQVAANMRAELLSVVGKRPIK 1078
L D QINA+ +E FG G+ETS +T EWA AEL KNP + A +R EL VVGK +K
Sbjct: 268 LLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVK 327
Query: 1079 ESDIPNMPYLQAFVKETLRLHPPTPLLVPRRALETCQVLNFTIPKECQIMVNAWGIGRDP 1258
ESD+P++PYLQA +KET+RLHP P L+P RA+ETC V+ +TIPK+ Q++VN + IGRDP
Sbjct: 328 ESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDP 387
Query: 1259 KNWTDPLKFSPERFLNS 1309
K W +P F PERFL S
Sbjct: 388 KTWKEPTTFKPERFLES 404