BLASTX nr result
ID: Papaver32_contig00006545
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00006545
(1124 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 697 0.0
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 694 0.0
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 693 0.0
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik... 616 0.0
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 584 0.0
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 582 0.0
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 579 0.0
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 541 0.0
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 532 0.0
AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum fla... 528 0.0
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 351 e-114
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 343 e-111
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 343 e-111
XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 341 e-110
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 340 e-110
AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum] 338 e-109
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 336 e-108
XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 330 e-106
XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 330 e-106
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 329 e-106
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 697 bits (1798), Expect = 0.0
Identities = 347/374 (92%), Positives = 354/374 (94%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PHSQFA+LA+TYGDLF+LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV
Sbjct: 43 PHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 102
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAE
Sbjct: 103 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAE 162
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDL
Sbjct: 163 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDL 222
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQ KDVADCLQGIY+VWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI
Sbjct: 223 FGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 282
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FGAGTETSASTIEWALSELTKNP+VTANMR ELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 283 FGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKET 342
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS
Sbjct: 343 LRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 402
Query: 1081 SIDFKGNDFELIPF 1122
SIDFKGNDFELIPF
Sbjct: 403 SIDFKGNDFELIPF 416
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 694 bits (1791), Expect = 0.0
Identities = 347/374 (92%), Positives = 352/374 (94%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV
Sbjct: 48 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 107
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWSECNETWKKLRKVCR ELFTQKMIESQAEIRE KAMEMVE+LK+N G+EVKI E
Sbjct: 108 ENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVE 167
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL
Sbjct: 168 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 227
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQRKDVADCLQGIY+VWGAMLKERKIAK HNNSKKNDFVEILLDSGLDDQQINALLMEI
Sbjct: 228 FGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEI 287
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 288 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKET 347
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKF+PERFLNS
Sbjct: 348 LRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNS 407
Query: 1081 SIDFKGNDFELIPF 1122
SIDFKGNDFELIPF
Sbjct: 408 SIDFKGNDFELIPF 421
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 693 bits (1789), Expect = 0.0
Identities = 345/374 (92%), Positives = 353/374 (94%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PHSQFA+LA+TYGDLF+LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV
Sbjct: 43 PHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 102
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWSECNETWKKLRKVCRT+LFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAE
Sbjct: 103 ENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAE 162
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDL
Sbjct: 163 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDL 222
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQ KDVADCLQGIY+VWGAMLKE KIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI
Sbjct: 223 FGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 282
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FGAGTETSASTIEWALSELTKNP+VTANMR ELLSVVGKRPVKESDIPNMPYLQAFVKE
Sbjct: 283 FGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKET 342
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS
Sbjct: 343 LRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 402
Query: 1081 SIDFKGNDFELIPF 1122
SIDFKGNDFELIPF
Sbjct: 403 SIDFKGNDFELIPF 416
>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
P450 [Papaver somniferum]
Length = 440
Score = 616 bits (1589), Expect = 0.0
Identities = 301/375 (80%), Positives = 333/375 (88%), Gaps = 1/375 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PHSQFA+LA+TYGDLFTLKLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HV
Sbjct: 49 PHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHV 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVW+ECNETWKKLRKVCRT+LFTQKMIE+QAE+RE K MEMV+YLKKN G EVKI E
Sbjct: 109 ENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVE 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
V+FGTLVN+FGNLIFSQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG DL
Sbjct: 169 VIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDL 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAK-QHNNSKKNDFVEILLDSGLDDQQINALLME 717
FGQRKDVADCLQG+Y VWGAMLKER+IAK Q N KNDFV++LLDSGLDDQQIN+LL++
Sbjct: 229 FGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLD 288
Query: 718 IFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE 897
+F AGTET+ASTIEWAL+ELTKNP+VTA++RSELLSVVGKR VKESDI N+PYLQAFVKE
Sbjct: 289 MFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKE 348
Query: 898 XXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN 1077
ETC+VLNYTIPKECQIMVNAWGIGRDPK WTDPLKFSP+RFLN
Sbjct: 349 TLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLN 408
Query: 1078 SSIDFKGNDFELIPF 1122
SSIDFKGNDFELIPF
Sbjct: 409 SSIDFKGNDFELIPF 423
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 584 bits (1505), Expect = 0.0
Identities = 284/375 (75%), Positives = 323/375 (86%), Gaps = 1/375 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HV
Sbjct: 48 PHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHV 107
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWS+C ETWK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI E
Sbjct: 108 ENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVE 167
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
V+FGTLVNIFGNLIFSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDL
Sbjct: 168 VIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDL 227
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSK-KNDFVEILLDSGLDDQQINALLME 717
FGQRK+VA+CL+GIY +WGAML+ERK+AK+ + K KNDFV++ LDSGL+D QINALLME
Sbjct: 228 FGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLME 287
Query: 718 IFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE 897
+FGAGTETSASTIEWA++ELTKNP++TA +RSE+ +VVG+R VKESD PN+PYL+A VKE
Sbjct: 288 LFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKE 347
Query: 898 XXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN 1077
ETC +LNYTIPK+CQIMVNAWGIGRDPK WTDPL FSPERFLN
Sbjct: 348 TLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLN 407
Query: 1078 SSIDFKGNDFELIPF 1122
SS+DF+GNDF LIPF
Sbjct: 408 SSVDFRGNDFSLIPF 422
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 582 bits (1499), Expect = 0.0
Identities = 283/375 (75%), Positives = 321/375 (85%), Gaps = 1/375 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HV
Sbjct: 49 PHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHV 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWS+C ETWK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI E
Sbjct: 109 ENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVE 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
V+FGTLVNIFGNLIFSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDL
Sbjct: 169 VIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDL 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSGLDDQQINALLME 717
FGQRK+VA+CL+GIY +WGAML+ERK+AK+ + KNDFV++ LDSGL+D QINALLME
Sbjct: 229 FGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLME 288
Query: 718 IFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE 897
+FGAGTETSASTIEWA++ELTKNP++TA +RSEL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 289 LFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKE 348
Query: 898 XXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN 1077
ETC +LNYTIPK+CQIMVNAWGIGRDPK W DPL FSPERFLN
Sbjct: 349 TLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLN 408
Query: 1078 SSIDFKGNDFELIPF 1122
SS+DF+GNDF LIPF
Sbjct: 409 SSVDFRGNDFSLIPF 423
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 579 bits (1492), Expect = 0.0
Identities = 282/375 (75%), Positives = 320/375 (85%), Gaps = 1/375 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HV
Sbjct: 49 PHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHV 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWS+C ETWK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI E
Sbjct: 109 ENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVE 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
V+FGTLVNIFGNLIFSQNIF+LG +SGS E KE+LWRMLELGNSTNPADYFP LG+FDL
Sbjct: 169 VIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDL 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSGLDDQQINALLME 717
FGQRK+VA+CL+GIY +WGAML+ERK+AK+ + KNDFV++ LDSGL+D QINALLME
Sbjct: 229 FGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLME 288
Query: 718 IFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKE 897
+FGAGTETSASTIEWA++ELTKNP++TA +RSEL +VVG+R VKESD PN+PYL+A VKE
Sbjct: 289 LFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKE 348
Query: 898 XXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN 1077
ETC +LNYTIPK+CQIMVNAWGIGRDPK W DPL FSPERFLN
Sbjct: 349 TLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLN 408
Query: 1078 SSIDFKGNDFELIPF 1122
SS+DF+GNDF LIPF
Sbjct: 409 SSVDFRGNDFSLIPF 423
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 541 bits (1395), Expect = 0.0
Identities = 261/374 (69%), Positives = 312/374 (83%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH++FA+LA+ YG+LF+LKLGS+TVVVAS+P AA+EILKTHD++LSGRYVFQSFRVKEHV
Sbjct: 49 PHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHV 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWSECN+ WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI E
Sbjct: 109 ENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVE 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVFGTLVNIFGNLIFS+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +G+ DL
Sbjct: 169 VVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDL 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQR+ VA+ LQ IY+VWG MLKER+ K + KNDFV++LL++GLDDQ+INALLME+
Sbjct: 229 FGQRRAVAEVLQQIYDVWGVMLKERRGTK--GSESKNDFVDVLLNAGLDDQKINALLMEL 286
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FGAGTETSASTIEWA++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 287 FGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKET 346
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S
Sbjct: 347 LRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSS 406
Query: 1081 SIDFKGNDFELIPF 1122
+D+KGNDFELIPF
Sbjct: 407 DVDYKGNDFELIPF 420
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 532 bits (1371), Expect = 0.0
Identities = 257/374 (68%), Positives = 308/374 (82%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
P ++FA+LA+ YG LF+LKLGS+TVVVAS+P AA+E+LKTHD++LSGRYVFQSFRVKEHV
Sbjct: 49 PRAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHV 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
ENSIVWSECN+ WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI E
Sbjct: 109 ENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVE 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVFGTLVNIFGNLIFS+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +G++DL
Sbjct: 169 VVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDL 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQR+ VAD LQ IY+VWG LKER+ K + NDFV++LL++GL+DQ+INALLME+
Sbjct: 229 FGQRRAVADVLQQIYDVWGINLKERRGMK--GSESNNDFVDVLLNAGLEDQKINALLMEL 286
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FG GTETSASTIEWA++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 287 FGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKET 346
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S
Sbjct: 347 LRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSS 406
Query: 1081 SIDFKGNDFELIPF 1122
+D+KGNDFELIPF
Sbjct: 407 DVDYKGNDFELIPF 420
>AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 528 bits (1359), Expect = 0.0
Identities = 253/374 (67%), Positives = 308/374 (82%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH++FA+LA+ YG+LFTLKLGS+TVVVAS+P AA+EILKT D++LSGRYVFQSFRV EHV
Sbjct: 50 PHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHV 109
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
NSIVWSECNE WK LRKVCRTELF+ KMIESQA IRE KA++MV +L+K EVKI E
Sbjct: 110 LNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVE 169
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
VVF TLVNIFGNLIFS+++F L D + GS E+KEHLW++L++GNSTNPADYFP +G+FDL
Sbjct: 170 VVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDL 229
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
FGQR++VA L+ IY+VWG MLKER+ H ++NDF +LL++GLDDQ+INALLME+
Sbjct: 230 FGQRREVAKVLKQIYDVWGVMLKERRSITGH---RENDFANVLLNAGLDDQKINALLMEL 286
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEX 900
FGAGTET+ASTIEWA++ELT N V + +R+EL++VVG + VKES+IP++PYLQA VKE
Sbjct: 287 FGAGTETTASTIEWAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKET 346
Query: 901 XXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNS 1080
ETCKV+NYTIPKECQIMVNAW IGRDPK W DPL F PERF+NS
Sbjct: 347 LRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNS 406
Query: 1081 SIDFKGNDFELIPF 1122
++D+KGNDFELIPF
Sbjct: 407 TVDYKGNDFELIPF 420
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 351 bits (900), Expect = e-114
Identities = 173/375 (46%), Positives = 254/375 (67%), Gaps = 1/375 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH + ++LA+ +G L LK G E VVVAS+ +AA E+LK DR+LSGR+ S R+K ++
Sbjct: 49 PHVELSKLAQRFGPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYI 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
E+S+VW++C + WK +RKV RTELF+ KM++ QA RE K E++++L + G +V A+
Sbjct: 109 EHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFAD 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDL 540
V+FG+++NI G LIFS++++ D + ++ MK + +++ L N AD +P LG D
Sbjct: 169 VIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDF 228
Query: 541 FGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEI 720
G RK A C++ + W A++K+R +++++ KNDF+++LLDSG D QI+A+L+E
Sbjct: 229 QGLRKASAACVKRMNESWAAIVKQR---RKNDDHSKNDFLQVLLDSGFSDPQIDAMLLET 285
Query: 721 FGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGK-RPVKESDIPNMPYLQAFVKE 897
FG G++TS STIEWA++EL +NPE +R EL V+ + VKESD+PN+PYL A VKE
Sbjct: 286 FGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKE 345
Query: 898 XXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN 1077
ETC+++NYTIPK CQ+MVN + IGRD K W PL F PERFLN
Sbjct: 346 TLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLN 405
Query: 1078 SSIDFKGNDFELIPF 1122
S +D++GNDF+ IPF
Sbjct: 406 SELDYQGNDFQYIPF 420
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 343 bits (881), Expect = e-111
Identities = 174/377 (46%), Positives = 240/377 (63%), Gaps = 3/377 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY+ S VK
Sbjct: 58 PHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPK 117
Query: 181 EN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVK 351
N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE+L G VK
Sbjct: 118 LNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVK 177
Query: 352 IAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGR 531
+ ++VF T+ NI N FS ++ DE +K+ + + E G + N +DY+P LG
Sbjct: 178 VMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGG 237
Query: 532 FDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALL 711
D+ G + + + I W +LKER+ ++ N S DF+E LL+ G +D QIN ++
Sbjct: 238 LDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEIGFEDDQINQVI 296
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
+E+F AG ETS+ T+EWA++EL +N + +R EL +VG+ PV+ES +P +PYLQA V
Sbjct: 297 LELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACV 356
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
KE ETC+V+ YTIPK+ QI VN W + RDPK W DPL F PERF
Sbjct: 357 KEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERF 416
Query: 1072 LNSSIDFKGNDFELIPF 1122
L+S +DFKGNDFE IPF
Sbjct: 417 LDSKLDFKGNDFEYIPF 433
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 343 bits (880), Expect = e-111
Identities = 174/377 (46%), Positives = 240/377 (63%), Gaps = 3/377 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY+ S VK
Sbjct: 58 PHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPK 117
Query: 181 EN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVK 351
N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE+L G VK
Sbjct: 118 LNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVK 177
Query: 352 IAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGR 531
+ ++VF T+ NI N FS ++ DE +K+ + + E G + N +DY+P LG
Sbjct: 178 VMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGG 237
Query: 532 FDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALL 711
D+ G + + + I W +LKER+ ++ N S DF+E LL+ G +D QIN ++
Sbjct: 238 LDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEIGFEDDQINQVI 296
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
+E+F AG ETS+ T+EWA++EL +N + +R EL +VG+ PV+ES +P +PYLQA V
Sbjct: 297 LELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACV 356
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
KE ETC+V+ YTIPK+ QI VN W + RDPK W DPL F PERF
Sbjct: 357 KEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERF 416
Query: 1072 LNSSIDFKGNDFELIPF 1122
L+S +DFKGNDFE IPF
Sbjct: 417 LDSKLDFKGNDFEYIPF 433
>XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus
communis] EEF51432.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 341 bits (874), Expect = e-110
Identities = 169/377 (44%), Positives = 242/377 (64%), Gaps = 4/377 (1%)
Frame = +1
Query: 4 HSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVE 183
H A AK YG L +L+LG++ +VVASTP +A+E+LK HDR+LS R++ ++ + H+
Sbjct: 57 HICIANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHIL 116
Query: 184 N--SIVWSE-CNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKI 354
+IVW+ CN+ WK LR +CRTELF+ K IESQA +RE K EM+E+L G V +
Sbjct: 117 ERVAIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNV 176
Query: 355 AEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRF 534
AEVVFGT+ N +L+FS ++ ++ G +K LW M+E+ S N A+++P L
Sbjct: 177 AEVVFGTIFNTISHLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPL 233
Query: 535 DLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSK-KNDFVEILLDSGLDDQQINALL 711
D G ++ + CL+ ++ VW +KER+ H+++ K DF++I L +G DD QIN L+
Sbjct: 234 DPQGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLV 293
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
ME+ AGT+T+++++EWA++EL KN E +R EL + K P+KES + +PYL A V
Sbjct: 294 MELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACV 353
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
KE E C+V+NYTIPK+ Q++VN W IGRDP W DP F PERF
Sbjct: 354 KETLRLHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERF 413
Query: 1072 LNSSIDFKGNDFELIPF 1122
L SS+D KG+DFELIPF
Sbjct: 414 LGSSLDVKGHDFELIPF 430
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 340 bits (871), Expect = e-110
Identities = 165/373 (44%), Positives = 243/373 (65%)
Frame = +1
Query: 4 HSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVE 183
H A +A+T+G + L LG++ V+AST AA EILKTHDR+ S R++ SFR+K H++
Sbjct: 53 HITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIK 112
Query: 184 NSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEV 363
S+VWS+C + WK LRK+ RTE+F+ KM+++Q+ +RE K E++++L+ G VKI++
Sbjct: 113 YSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQF 172
Query: 364 VFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLF 543
VFGTL+NI GN++FS+++F GDE+ ++ + ML +G N A+++P L DL
Sbjct: 173 VFGTLLNILGNVVFSKDVFVYGDETDKG-GIQNLIREMLMIGAEPNVAEFYPSLEELDLQ 231
Query: 544 GQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIF 723
G +K + + +W +KER K + N + D +++LL + +D QINAL +E F
Sbjct: 232 GLKKKCDERFIRVMKMWEGTVKER---KANRNEESKDMLDVLLANDFNDAQINALFLETF 288
Query: 724 GAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXX 903
G G+ETS++TIEW ++EL K+P+ A +R EL VVG +KESD+P +PYLQA +KE
Sbjct: 289 GPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAM 348
Query: 904 XXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSS 1083
ETC+V+ YTIPK Q++VNA+ IGRDPK W DP F PERFL S
Sbjct: 349 RLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESD 408
Query: 1084 IDFKGNDFELIPF 1122
+DF G ++ IPF
Sbjct: 409 VDFHGAHYQFIPF 421
>AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 338 bits (867), Expect = e-109
Identities = 169/373 (45%), Positives = 241/373 (64%)
Frame = +1
Query: 4 HSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVE 183
H +A+T+G + L LG++ VAST AA EILKTHDR SGR V SFR+K H++
Sbjct: 51 HVALTNMARTHGPIMILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIK 110
Query: 184 NSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEV 363
S+VW++C+E WK LRK+ RTE+F+ KM++ Q +RE K E+VE+LK G VKI+++
Sbjct: 111 YSLVWADCSEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQL 170
Query: 364 VFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLF 543
VFGTL+NI GN+IFS+++F+ + +M+ + ML +G + N A+++P FDL
Sbjct: 171 VFGTLLNILGNVIFSKDVFRY--VGADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQ 228
Query: 544 GQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIF 723
G +K + +W ++ERK+ + N + D +++LL + +D QINA+ +E F
Sbjct: 229 GLKKRCNQRFSEVMKMWKGTVEERKVKR---NEEPKDMLDVLLLNDFNDAQINAVFLETF 285
Query: 724 GAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKEXX 903
G G+ETS++T EWAL+EL KNP + A +R EL VVGK VKESD+P++PYLQA +KE
Sbjct: 286 GPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETM 345
Query: 904 XXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSS 1083
ETC V+ YTIPK+ Q++VN + IGRDPK W +P F PERFL S
Sbjct: 346 RLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESD 405
Query: 1084 IDFKGNDFELIPF 1122
+D+ G FE IPF
Sbjct: 406 VDYYGKHFEFIPF 418
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 336 bits (862), Expect = e-108
Identities = 169/377 (44%), Positives = 238/377 (63%), Gaps = 3/377 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH + A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRYV S VK+
Sbjct: 58 PHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPK 117
Query: 181 ENSIVWS---ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVK 351
N + + EC WK LR +CRTE+F+ K +ES E+RE K ME+VE+L G VK
Sbjct: 118 LNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVK 177
Query: 352 IAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGR 531
+ ++VF T+ NI N FS ++ E +K+ ++R ELG + N +D++P L
Sbjct: 178 VMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATPNLSDFYPILDG 232
Query: 532 FDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALL 711
+L G +K + L I W LKER+ K +S + D +E L+ +D QIN ++
Sbjct: 233 LNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHR-DLLEAFLEIRFEDDQINQVI 291
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
+E+F AG +TS TIEWA+++L +NP+V +R EL ++G+ PV+ES +P++PYLQA V
Sbjct: 292 LELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACV 351
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
KE ETC+V+ YTIPK+ Q+ VN W +GRDPK W DPL F+PERF
Sbjct: 352 KETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERF 411
Query: 1072 LNSSIDFKGNDFELIPF 1122
L+S ++FKGNDFE IPF
Sbjct: 412 LDSKLEFKGNDFEYIPF 428
>XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 330 bits (847), Expect = e-106
Identities = 163/376 (43%), Positives = 247/376 (65%), Gaps = 2/376 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
P+ + +LA+ YG L + G E VVVAS +AA E+LK DRVLSGR+ S RVK ++
Sbjct: 49 PNLELFKLAQRYGPLMLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYI 108
Query: 181 ENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAE 360
E S+VW++C + WK +RK+ RTELF+ KM++ A +RE K E++++L++ G EV +
Sbjct: 109 EYSMVWADCTDYWKMVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVD 168
Query: 361 VVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFP-FLGRFD 537
V+FG ++N+ G LI+S++++ D++ ++ MK + +++ L + N AD +P F D
Sbjct: 169 VIFGCILNMLGALIYSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSD 228
Query: 538 LFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLME 717
G RK+ A C++ + W A++ ER +++N+ KND +++LLDSG D QI+A+ +E
Sbjct: 229 FQGLRKESAACVKRMSESWAAIINER---RKNNDHTKNDLLQVLLDSGFSDPQIDAIFLE 285
Query: 718 IFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGK-RPVKESDIPNMPYLQAFVK 894
FG G++TSASTIEWAL+EL +NPE + EL V+G+ VK+SD+PN+PYL A VK
Sbjct: 286 TFGPGSDTSASTIEWALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVK 345
Query: 895 EXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFL 1074
E E+C+V+NYTIPK +++VN + IGRDP W +P F PERFL
Sbjct: 346 ETLRLHPPVPFLIPHIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTWDNPNSFLPERFL 405
Query: 1075 NSSIDFKGNDFELIPF 1122
NS +D++GN F+ IPF
Sbjct: 406 NSEVDYQGNHFQYIPF 421
>XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 330 bits (845), Expect = e-106
Identities = 163/377 (43%), Positives = 243/377 (64%), Gaps = 3/377 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH A AKT+G L +LKLG + ++V S+ AA+EILK+HDR+LS RYVF++ + HV
Sbjct: 53 PHISTANFAKTHGPLISLKLGKQLLIVGSSKRAATEILKSHDRLLSARYVFKAALFENHV 112
Query: 181 EN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVK 351
+ SIVW+ +C++ WK LR +CR ELF+ K IESQA +RE K EMVE++ + G V
Sbjct: 113 LDRISIVWATQCSDGWKSLRALCRNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVG 172
Query: 352 IAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGR 531
I EVV + N NL+FS ++ L D+ + + +K +WRM++LG + N +D++P LG
Sbjct: 173 IGEVVLAIVFNTIANLLFSVDLIGLEDDGAAT-GLKSLMWRMMKLGATPNISDFYPILGG 231
Query: 532 FDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALL 711
D G ++ +A C+ ++ +WG +KER+ K ++ ++DF+++ L +G +D QIN L
Sbjct: 232 IDPQGLKRKMAVCVNQMFGIWGKYIKERR-EKHVHDGPRSDFLDVFLANGFEDLQINWLA 290
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
+E+ AGT+T+A+T+EWA++EL KN EV + E+ +KES + +PYL A V
Sbjct: 291 LELLSAGTDTTATTVEWAIAELLKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACV 350
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
E ETCKV++YTIP++ +++VN W +GRDP W DPL F PERF
Sbjct: 351 NETLRLHPPVPFLIPRRALETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERF 410
Query: 1072 LNSSIDFKGNDFELIPF 1122
L S +DFKG+DFE +PF
Sbjct: 411 LGSDLDFKGHDFEFLPF 427
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 329 bits (843), Expect = e-106
Identities = 166/377 (44%), Positives = 243/377 (64%), Gaps = 3/377 (0%)
Frame = +1
Query: 1 PHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHV 180
PH A AK +G L +L+LG+ VVVAS+P AA+EILKTHDR+LSGRY+ + +++V
Sbjct: 57 PHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNV 116
Query: 181 ENSI--VWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVK 351
+ I VW+ C++ WK LR +CR+ELF+ K IESQA +RE K EM+++L G V
Sbjct: 117 LDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVN 176
Query: 352 IAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGR 531
I EVVF T N NL+FS+++ D+ + E+K + ++EL N AD++P L +
Sbjct: 177 IGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLISTLMELATCPNIADFYPVLTK 235
Query: 532 FDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALL 711
D G ++ + +CL+ ++ VW +KER+ + +++K DF+++ L +G DD QIN LL
Sbjct: 236 LDPQGIKRKMKNCLERMFGVWDIYIKERR-ERHVKDARKTDFLDVFLSNGFDDHQINWLL 294
Query: 712 MEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFV 891
+E+F AG +T+ +T+EWA++EL K V +R EL + +GK ++ES IP + YL A V
Sbjct: 295 LELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACV 354
Query: 892 KEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF 1071
KE E C+V+NYTIPK QI+VN W IGRDP W DPL + PERF
Sbjct: 355 KETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERF 414
Query: 1072 LNSSIDFKGNDFELIPF 1122
L+S++DFKG++FE +PF
Sbjct: 415 LDSNLDFKGHNFEFLPF 431