BLASTX nr result
ID: Papaver32_contig00006544
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver32_contig00006544
(1191 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 754 0.0
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 732 0.0
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 729 0.0
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik... 657 0.0
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 628 0.0
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 626 0.0
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 624 0.0
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 582 0.0
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 573 0.0
AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum fla... 564 0.0
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 384 e-127
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 383 e-127
XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 378 e-124
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 376 e-124
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 374 e-123
XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 371 e-122
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 366 e-120
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 365 e-119
AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum] 364 e-119
XP_012076022.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 361 e-118
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 754 bits (1947), Expect = 0.0
Identities = 376/396 (94%), Positives = 378/396 (95%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL
Sbjct: 26 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 85
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWKKLRKVCRAELFTQKMIESQAEIRE
Sbjct: 86 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRE 145
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR
Sbjct: 146 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 205
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
MLELGNSTNPADYFPFLGRFDLFGQRKDV DCLQGIYSVWGAMLKERKIAKLHNNSKKND
Sbjct: 206 MLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKND 265
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG
Sbjct: 266 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 325
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
KRPVKESDIPNMPYLQAFVKE ETCKVLNYTIPKECQIMVNAWG
Sbjct: 326 KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 385
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPKRWTDPLKF+PERFLNSSIDFKGNDFELIPF
Sbjct: 386 IGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPF 421
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 732 bits (1890), Expect = 0.0
Identities = 362/396 (91%), Positives = 372/396 (93%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
KGLPPGPKPWPIVGNLLQLGEKPHSQFA+LA+TYGDLF+LKLGSETVVVASTPLAASEIL
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEIL 80
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWKKLRKVCR ELFTQKMIESQAE+RE
Sbjct: 81 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRE 140
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
SKAMEMVE+LK+N G+EVKI EVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR
Sbjct: 141 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 200
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
MLELGNSTNPADYFPFLG+FDLFGQ KDV DCLQGIYSVWGAMLKERKIAK HNNSKKND
Sbjct: 201 MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKND 260
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNP+VTANMR ELLSVVG
Sbjct: 261 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVG 320
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
KRPVKESDIPNMPYLQAFVKE ETCKVLNYTIPKECQIMVNAWG
Sbjct: 321 KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF
Sbjct: 381 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 416
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 729 bits (1881), Expect = 0.0
Identities = 360/396 (90%), Positives = 371/396 (93%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
KGLPPGPKPWPIVGNLLQLGEKPHSQFA+LA+TYGDLF+LKLGSETVVVASTPLAASEIL
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEIL 80
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWKKLRKVCR +LFTQKMIESQAE+RE
Sbjct: 81 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRE 140
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
SKAMEMVE+LK+N G+EVKI EVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR
Sbjct: 141 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 200
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
MLELGNSTNPADYFPFLG+FDLFGQ KDV DCLQGIYSVWGAMLKE KIAK HNNSKKND
Sbjct: 201 MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKND 260
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNP+VTANMR ELLSVVG
Sbjct: 261 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVG 320
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
KRPVKESDIPNMPYLQAFVKE ETCKVLNYTIPKECQIMVNAWG
Sbjct: 321 KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF
Sbjct: 381 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 416
>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
P450 [Papaver somniferum]
Length = 440
Score = 657 bits (1694), Expect = 0.0
Identities = 317/396 (80%), Positives = 353/396 (89%), Gaps = 1/396 (0%)
Frame = +3
Query: 6 GLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILK 185
GLPPGPKPWPIVGNLLQLGEKPHSQFA+LA+TYGDLFTLKLG++TVVVASTPLAASE+LK
Sbjct: 28 GLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLK 87
Query: 186 THDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRES 365
HDR L GRYVFQSFRVK HVENSIVW+ECN+TWKKLRKVCR +LFTQKMIE+QAE+RE
Sbjct: 88 AHDRTLCGRYVFQSFRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREI 147
Query: 366 KAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRM 545
K MEMV++LK+N+G EVKIVEV+FGTLVN+FGNLIFSQ+IFKLGDESSGS+EMK+H+WRM
Sbjct: 148 KTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRM 207
Query: 546 LELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLH-NNSKKND 722
LELGNS NP DY P LG DLFGQRKDV DCLQG+Y VWGAMLKER+IAK N KND
Sbjct: 208 LELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKND 267
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FV++LLDSGLDDQQIN+LL+++F AGTET+ASTIEWAL+ELTKNP+VTA++RSELLSVVG
Sbjct: 268 FVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVG 327
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
KR VKESDI N+PYLQAFVKE ETC+VLNYTIPKECQIMVNAWG
Sbjct: 328 KRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWG 387
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPK WTDPLKFSP+RFLNSSIDFKGNDFELIPF
Sbjct: 388 IGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPF 423
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 628 bits (1619), Expect = 0.0
Identities = 302/397 (76%), Positives = 344/397 (86%), Gaps = 1/397 (0%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGPKPWPIVGNLLQLGEKPH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEIL
Sbjct: 26 KNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEIL 85
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK LRKVCR ELFTQKMIESQA +RE
Sbjct: 86 KTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVRE 145
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
K EMVE+L + QG EVKIVEV+FGTLVNIFGNLIFSQNIF+LGD +SGS E KE+LWR
Sbjct: 146 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWR 205
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSK-KN 719
MLELGNSTNPADYFP LG+FDLFGQRK+V +CL+GIY++WGAML+ERK+AK + K KN
Sbjct: 206 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKN 265
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DFV++ LDSGL+D QINALLME+FGAGTETSASTIEWA++ELTKNP++TA +RSE+ +VV
Sbjct: 266 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVV 325
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+R VKESD PN+PYL+A VKE ETC +LNYTIPK+CQIMVNAW
Sbjct: 326 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 385
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
GIGRDPK WTDPL FSPERFLNSS+DF+GNDF LIPF
Sbjct: 386 GIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPF 422
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 626 bits (1615), Expect = 0.0
Identities = 301/397 (75%), Positives = 342/397 (86%), Gaps = 1/397 (0%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGPKPWPIVGNLLQLGEKPH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEIL
Sbjct: 27 KNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEIL 86
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK LRKVCR ELFTQKMIESQA +RE
Sbjct: 87 KTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVRE 146
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
K EMVE+L + QG EVKIVEV+FGTLVNIFGNLIFSQNIF+LGD +SGS E KE+LWR
Sbjct: 147 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWR 206
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIA-KLHNNSKKN 719
MLELGNSTNPADYFP LG+FDLFGQRK+V +CL+GIY++WGAML+ERK+A K+ KN
Sbjct: 207 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKN 266
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DFV++ LDSGL+D QINALLME+FGAGTETSASTIEWA++ELTKNP++TA +RSEL +VV
Sbjct: 267 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVV 326
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+R VKESD PN+PYL+A VKE ETC +LNYTIPK+CQIMVNAW
Sbjct: 327 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 386
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
GIGRDPK W DPL FSPERFLNSS+DF+GNDF LIPF
Sbjct: 387 GIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPF 423
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 624 bits (1608), Expect = 0.0
Identities = 300/397 (75%), Positives = 341/397 (85%), Gaps = 1/397 (0%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGPKPWPIVGNLLQLGEKPH+QFAELA+TYGD+FTLK+G+ETVVVAST AASEIL
Sbjct: 27 KNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEIL 86
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHDR+LS RYVFQSFRVK HVENSIVWS+C +TWK LRKVCR ELFTQKMIESQA +RE
Sbjct: 87 KTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVRE 146
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
K EMVE+L + QG EVKIVEV+FGTLVNIFGNLIFSQNIF+LG +SGS E KE+LWR
Sbjct: 147 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWR 206
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIA-KLHNNSKKN 719
MLELGNSTNPADYFP LG+FDLFGQRK+V +CL+GIY++WGAML+ERK+A K+ KN
Sbjct: 207 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKN 266
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DFV++ LDSGL+D QINALLME+FGAGTETSASTIEWA++ELTKNP++TA +RSEL +VV
Sbjct: 267 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVV 326
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+R VKESD PN+PYL+A VKE ETC +LNYTIPK+CQIMVNAW
Sbjct: 327 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 386
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
GIGRDPK W DPL FSPERFLNSS+DF+GNDF LIPF
Sbjct: 387 GIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPF 423
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 582 bits (1500), Expect = 0.0
Identities = 280/396 (70%), Positives = 332/396 (83%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG+LF+LKLGS+TVVVAS+P AA+EIL
Sbjct: 27 KNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEIL 86
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHD++LSGRYVFQSFRVKEHVENSIVWSECND WK LRKVCR ELFT KMIESQ+EIRE
Sbjct: 87 KTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIRE 146
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
+KA EMV+FL+ +G VKIVEVVFGTLVNIFGNLIFS+++F L D + GSVE+KEHLW+
Sbjct: 147 AKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWK 206
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
+L++GNSTNPADYFP +G+ DLFGQR+ V + LQ IY VWG MLKER+ K + KND
Sbjct: 207 LLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTK--GSESKND 264
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FV++LL++GLDDQ+INALLME+FGAGTETSASTIEWA++ELTK P V + +R EL++VVG
Sbjct: 265 FVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 324
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
VKESD+P++PYLQAFVKE ETC V+NYTIPKECQIMVNAW
Sbjct: 325 DNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWA 384
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPK W DPL F PERFL+S +D+KGNDFELIPF
Sbjct: 385 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 573 bits (1478), Expect = 0.0
Identities = 276/396 (69%), Positives = 327/396 (82%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGP+P PIVGNLLQLG+KP ++FA+LA+ YG LF+LKLGS+TVVVAS+P AA+E+L
Sbjct: 27 KNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVL 86
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KTHD++LSGRYVFQSFRVKEHVENSIVWSECND WK LRKVCR ELFT KMIESQ+EIRE
Sbjct: 87 KTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIRE 146
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
KA EMV+FL+ +G VKIVEVVFGTLVNIFGNLIFS+++F L D + GSVE+KEHLW+
Sbjct: 147 GKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWK 206
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
+LE+GNSTNPADYFP +G++DLFGQR+ V D LQ IY VWG LKER+ K + ND
Sbjct: 207 LLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMK--GSESNND 264
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
FV++LL++GL+DQ+INALLME+FG GTETSASTIEWA++ELTK P V + +R EL++VVG
Sbjct: 265 FVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 324
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
VKESD+P++PYLQAFVKE ETC V+NYTIPKECQIMVNAW
Sbjct: 325 DNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWA 384
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPK W DPL F PERFL+S +D+KGNDFELIPF
Sbjct: 385 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
>AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 564 bits (1454), Expect = 0.0
Identities = 270/396 (68%), Positives = 328/396 (82%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
K LPPGP+P PIVGNLLQLGEKPH++FA+LA+ YG+LFTLKLGS+TVVVAS+P AA+EIL
Sbjct: 28 KDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEIL 87
Query: 183 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
KT D++LSGRYVFQSFRV EHV NSIVWSECN+ WK LRKVCR ELF+ KMIESQA IRE
Sbjct: 88 KTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIRE 147
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
+KA++MV FL++ + EVKIVEVVF TLVNIFGNLIFS+++F L D + GS E+KEHLW+
Sbjct: 148 AKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHLWK 207
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
+L++GNSTNPADYFP +G+FDLFGQR++V L+ IY VWG MLKER+ H ++ND
Sbjct: 208 LLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGH---REND 264
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
F +LL++GLDDQ+INALLME+FGAGTET+ASTIEWA++ELT N V + +R+EL++VVG
Sbjct: 265 FANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVG 324
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
+ VKES+IP++PYLQA VKE ETCKV+NYTIPKECQIMVNAW
Sbjct: 325 HKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWA 384
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDPK W DPL F PERF+NS++D+KGNDFELIPF
Sbjct: 385 IGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPF 420
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 384 bits (985), Expect = e-127
Identities = 190/397 (47%), Positives = 260/397 (65%), Gaps = 3/397 (0%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKT 188
LPPGP PWPI+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKT
Sbjct: 38 LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKT 97
Query: 189 HDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNDTWKKLRKVCRAELFTQKMIESQAEIR 359
HDR LSGRY+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+R
Sbjct: 98 HDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELR 157
Query: 360 ESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLW 539
E K E+VEFL +G VK++++VF T+ NI N FS ++ DE +K+ +
Sbjct: 158 ERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIH 217
Query: 540 RMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKN 719
+ E G + N +DY+P LG D+ G + + + I + W +LKER+ + N S
Sbjct: 218 KNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQR-SNRSSHR 276
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DF+E LL+ G +D QIN +++E+F AG ETS+ T+EWA++EL +N + +R EL +V
Sbjct: 277 DFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIV 336
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+ PV+ES +P +PYLQA VKE ETC+V+ YTIPK+ QI VN W
Sbjct: 337 GESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIW 396
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
+ RDPK W DPL F PERFL+S +DFKGNDFE IPF
Sbjct: 397 AMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPF 433
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 383 bits (984), Expect = e-127
Identities = 190/397 (47%), Positives = 260/397 (65%), Gaps = 3/397 (0%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKT 188
LPPGP PWPI+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKT
Sbjct: 38 LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKT 97
Query: 189 HDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNDTWKKLRKVCRAELFTQKMIESQAEIR 359
HDR LSGRY+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+R
Sbjct: 98 HDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELR 157
Query: 360 ESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLW 539
E K E+VEFL +G VK++++VF T+ NI N FS ++ DE +K+ +
Sbjct: 158 ERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIH 217
Query: 540 RMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKN 719
+ E G + N +DY+P LG D+ G + + + I + W +LKER+ + N S
Sbjct: 218 KNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQR-SNRSSHR 276
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DF+E LL+ G +D QIN +++E+F AG ETS+ T+EWA++EL +N + +R EL +V
Sbjct: 277 DFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIV 336
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+ PV+ES +P +PYLQA VKE ETC+V+ YTIPK+ QI VN W
Sbjct: 337 GESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIW 396
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
+ RDPK W DPL F PERFL+S +DFKGNDFE IPF
Sbjct: 397 AMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPF 433
>XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus
communis] EEF51432.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 378 bits (970), Expect = e-124
Identities = 185/398 (46%), Positives = 260/398 (65%), Gaps = 4/398 (1%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKT 188
LPPGPKPWPI+GN+LQ+ +K H A AK YG L +L+LG++ +VVASTP +A+E+LK
Sbjct: 36 LPPGPKPWPILGNILQVDKKLHICIANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKN 95
Query: 189 HDRVLSGRYVFQSFRVKEHV--ENSIVWS-ECNDTWKKLRKVCRAELFTQKMIESQAEIR 359
HDR+LS R++ ++ + H+ +IVW+ CND WK LR +CR ELF+ K IESQA +R
Sbjct: 96 HDRLLSARFILKAIPSESHILERVAIVWNPACNDHWKSLRALCRTELFSPKAIESQAILR 155
Query: 360 ESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLW 539
E K EM+EFL QG V + EVVFGT+ N +L+FS ++ ++ G +K LW
Sbjct: 156 EKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTISHLLFSTDLIGFENQIGG---VKSLLW 212
Query: 540 RMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNN-SKK 716
M+E+ S N A+++P L D G ++ + CL+ ++ VW +KER+ H++ + K
Sbjct: 213 SMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPK 272
Query: 717 NDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSV 896
DF++I L +G DD QIN L+ME+ AGT+T+++++EWA++EL KN E +R EL
Sbjct: 273 TDFLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDRE 332
Query: 897 VGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNA 1076
+ K P+KES + +PYL A VKE E C+V+NYTIPK+ Q++VN
Sbjct: 333 INKNPIKESHVSQLPYLNACVKETLRLHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNV 392
Query: 1077 WGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
W IGRDP W DP F PERFL SS+D KG+DFELIPF
Sbjct: 393 WAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELIPF 430
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 376 bits (966), Expect = e-124
Identities = 185/397 (46%), Positives = 258/397 (64%), Gaps = 3/397 (0%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKT 188
LPPGP PWPI+GNLLQLG+ PH + A LAK +G L +L+LG++ +VVAS+P AA E+LKT
Sbjct: 38 LPPGPYPWPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKT 97
Query: 189 HDRVLSGRYVFQSFRVKEHVENSIVWS---ECNDTWKKLRKVCRAELFTQKMIESQAEIR 359
HDR LSGRYV S VK+ N + + EC + WK LR +CR E+F+ K +ES E+R
Sbjct: 98 HDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELR 157
Query: 360 ESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLW 539
E K ME+VEFL +G VK++++VF T+ NI N FS ++ E +K+ ++
Sbjct: 158 ERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIY 212
Query: 540 RMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKN 719
R ELG + N +D++P L +L G +K + L I + W LKER+ K + S
Sbjct: 213 RAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQK-NPGSSHR 271
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
D +E L+ +D QIN +++E+F AG +TS TIEWA+++L +NP+V +R EL ++
Sbjct: 272 DLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKII 331
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
G+ PV+ES +P++PYLQA VKE ETC+V+ YTIPK+ Q+ VN W
Sbjct: 332 GESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIW 391
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
+GRDPK W DPL F+PERFL+S ++FKGNDFE IPF
Sbjct: 392 AMGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPF 428
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 374 bits (961), Expect = e-123
Identities = 185/396 (46%), Positives = 267/396 (67%), Gaps = 2/396 (0%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLL-QLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILK 185
LPPGP WPI+G LL +L ++PH + ++LA+ +G L LK G E VVVAS+ +AA E+LK
Sbjct: 28 LPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRFGPLMLLKFGVEPVVVASSHVAAVEVLK 87
Query: 186 THDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAEIRES 365
DR+LSGR+ S R+K ++E+S+VW++C D WK +RKV R ELF+ KM++ QA RE
Sbjct: 88 NQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREE 147
Query: 366 KAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRM 545
K E+++FL R +G +V +V+FG+++NI G LIFS++++ D + ++ MK + ++
Sbjct: 148 KVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQL 207
Query: 546 LELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKNDF 725
+ L N AD +P LG D G RK C++ + W A++K+R + +++ KNDF
Sbjct: 208 MILAAIPNLADLYPILGGSDFQGLRKASAACVKRMNESWAAIVKQR---RKNDDHSKNDF 264
Query: 726 VEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGK 905
+++LLDSG D QI+A+L+E FG G++TS STIEWA++EL +NPE +R EL V+ +
Sbjct: 265 LQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRR 324
Query: 906 -RPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
VKESD+PN+PYL A VKE ETC+++NYTIPK CQ+MVN +
Sbjct: 325 SNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYA 384
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRD K W PL F PERFLNS +D++GNDF+ IPF
Sbjct: 385 IGRDSKTWEKPLSFLPERFLNSELDYQGNDFQYIPF 420
>XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 371 bits (953), Expect = e-122
Identities = 180/399 (45%), Positives = 262/399 (65%), Gaps = 3/399 (0%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEIL 182
+ LPPGPKPWPI+GN+L G+KPH A AKT+G L +LKLG + ++V S+ AA+EIL
Sbjct: 31 QSLPPGPKPWPIIGNILHFGKKPHISTANFAKTHGPLISLKLGKQLLIVGSSKRAATEIL 90
Query: 183 KTHDRVLSGRYVFQSFRVKEHVEN--SIVW-SECNDTWKKLRKVCRAELFTQKMIESQAE 353
K+HDR+LS RYVF++ + HV + SIVW ++C+D WK LR +CR ELF+ K IESQA
Sbjct: 91 KSHDRLLSARYVFKAALFENHVLDRISIVWATQCSDGWKSLRALCRNELFSAKAIESQAV 150
Query: 354 IRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEH 533
+RE K EMVEF+ R +G V I EVV + N NL+FS ++ L D+ + + +K
Sbjct: 151 LREKKMGEMVEFIGRRKGEVVGIGEVVLAIVFNTIANLLFSVDLIGLEDDGA-ATGLKSL 209
Query: 534 LWRMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSK 713
+WRM++LG + N +D++P LG D G ++ + C+ ++ +WG +KER+ +H +
Sbjct: 210 MWRMMKLGATPNISDFYPILGGIDPQGLKRKMAVCVNQMFGIWGKYIKERREKHVH-DGP 268
Query: 714 KNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLS 893
++DF+++ L +G +D QIN L +E+ AGT+T+A+T+EWA++EL KN EV + E+
Sbjct: 269 RSDFLDVFLANGFEDLQINWLALELLSAGTDTTATTVEWAIAELLKNKEVLKKVSEEIKR 328
Query: 894 VVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVN 1073
+KES + +PYL A V E ETCKV++YTIP++ +++VN
Sbjct: 329 ETDTNSLKESHVSQLPYLNACVNETLRLHPPVPFLIPRRALETCKVMDYTIPRDSEVIVN 388
Query: 1074 AWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
W +GRDP W DPL F PERFL S +DFKG+DFE +PF
Sbjct: 389 VWAVGRDPSLWEDPLSFKPERFLGSDLDFKGHDFEFLPF 427
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 366 bits (939), Expect = e-120
Identities = 181/397 (45%), Positives = 263/397 (66%), Gaps = 3/397 (0%)
Frame = +3
Query: 9 LPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKT 188
LPPGP+ WPI+GN+LQ+G+ PH A AK +G L +L+LG+ VVVAS+P AA+EILKT
Sbjct: 37 LPPGPRQWPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKT 96
Query: 189 HDRVLSGRYVFQSFRVKEHVEN--SIVWS-ECNDTWKKLRKVCRAELFTQKMIESQAEIR 359
HDR+LSGRY+ + +++V + ++VW+ C+D WK LR +CR+ELF+ K IESQA +R
Sbjct: 97 HDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLR 156
Query: 360 ESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLW 539
E K EM++FL QG V I EVVF T N NL+FS+++ D+ + E+K +
Sbjct: 157 EKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLIS 215
Query: 540 RMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKN 719
++EL N AD++P L + D G ++ + +CL+ ++ VW +KER+ + +++K
Sbjct: 216 TLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHV-KDARKT 274
Query: 720 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVV 899
DF+++ L +G DD QIN LL+E+F AG +T+ +T+EWA++EL K V +R EL + +
Sbjct: 275 DFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEI 334
Query: 900 GKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAW 1079
GK ++ES IP + YL A VKE E C+V+NYTIPK QI+VN W
Sbjct: 335 GKDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVW 394
Query: 1080 GIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
IGRDP W DPL + PERFL+S++DFKG++FE +PF
Sbjct: 395 AIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPF 431
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 365 bits (936), Expect = e-119
Identities = 181/400 (45%), Positives = 259/400 (64%), Gaps = 4/400 (1%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQL---GEKP-HSQFAELAKTYGDLFTLKLGSETVVVASTPLAA 170
K LPPGP PWP++GNL L E P H A +A+T+G + L LG++ V+AST AA
Sbjct: 26 KNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAA 85
Query: 171 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQA 350
EILKTHDR+ S R++ SFR+K H++ S+VWS+C D WK LRK+ R E+F+ KM+++Q+
Sbjct: 86 MEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQS 145
Query: 351 EIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKE 530
+RE K E+++FL+ +G VKI + VFGTL+NI GN++FS+++F GDE+ ++
Sbjct: 146 HVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKG-GIQN 204
Query: 531 HLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNS 710
+ ML +G N A+++P L DL G +K + + +W +KER K + N
Sbjct: 205 LIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKER---KANRNE 261
Query: 711 KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELL 890
+ D +++LL + +D QINAL +E FG G+ETS++TIEW ++EL K+P+ A +R EL
Sbjct: 262 ESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELN 321
Query: 891 SVVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMV 1070
VVG +KESD+P +PYLQA +KE ETC+V+ YTIPK Q++V
Sbjct: 322 EVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLV 381
Query: 1071 NAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
NA+ IGRDPK W DP F PERFL S +DF G ++ IPF
Sbjct: 382 NAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIPF 421
>AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 364 bits (934), Expect = e-119
Identities = 182/399 (45%), Positives = 256/399 (64%), Gaps = 3/399 (0%)
Frame = +3
Query: 3 KGLPPGPKPWPIVGNLLQLGEKP---HSQFAELAKTYGDLFTLKLGSETVVVASTPLAAS 173
K LPPGPKPWPI+GN+ L H +A+T+G + L LG++ VAST AA
Sbjct: 25 KNLPPGPKPWPIIGNIPALFSSAYPMHVALTNMARTHGPIMILWLGTQPTAVASTTEAAM 84
Query: 174 EILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAELFTQKMIESQAE 353
EILKTHDR SGR V SFR+K H++ S+VW++C++ WK LRK+ R E+F+ KM++ Q
Sbjct: 85 EILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLRKIVRTEIFSPKMLQVQEH 144
Query: 354 IRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEH 533
+RE K E+VEFLK +G VKI ++VFGTL+NI GN+IFS+++F+ + +M+
Sbjct: 145 VREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRY--VGADGDKMQTL 202
Query: 534 LWRMLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSK 713
+ ML +G + N A+++P FDL G +K + +W ++ERK+ + N +
Sbjct: 203 IKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKR---NEE 259
Query: 714 KNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLS 893
D +++LL + +D QINA+ +E FG G+ETS++T EWAL+EL KNP + A +R EL
Sbjct: 260 PKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQ 319
Query: 894 VVGKRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVN 1073
VVGK VKESD+P++PYLQA +KE ETC V+ YTIPK+ Q++VN
Sbjct: 320 VVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVN 379
Query: 1074 AWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
+ IGRDPK W +P F PERFL S +D+ G FE IPF
Sbjct: 380 IYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPF 418
>XP_012076022.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Jatropha curcas] XP_012092884.1 PREDICTED: probable
(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Jatropha
curcas]
Length = 502
Score = 361 bits (927), Expect = e-118
Identities = 180/396 (45%), Positives = 252/396 (63%), Gaps = 3/396 (0%)
Frame = +3
Query: 12 PPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTH 191
PPGP+PWPI+GN+ + EKPH A+LAK +G +F+L+LG + VVV S+P A+EIL+
Sbjct: 44 PPGPRPWPIIGNIPHVREKPHISMAKLAKLHGPIFSLRLGYQLVVVGSSPAVAAEILRKK 103
Query: 192 DRVLSGRYVFQSFRVKEHVENSI--VWS-ECNDTWKKLRKVCRAELFTQKMIESQAEIRE 362
DR LSGR+V ++ H + I VW+ C+D WK LR + R ELF+ K IES+A +RE
Sbjct: 104 DRFLSGRFVPKAIPRPSHELDRIALVWNPSCSDQWKSLRALSRTELFSAKAIESKAALRE 163
Query: 363 SKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 542
K EM+EFL QG V + EVVF T+ N NL+FS++ DE S S +K +W+
Sbjct: 164 QKVSEMIEFLGIKQGQVVNVGEVVFATVFNSISNLLFSKDFISFEDEESVS-GLKGLMWK 222
Query: 543 MLELGNSTNPADYFPFLGRFDLFGQRKDVVDCLQGIYSVWGAMLKERKIAKLHNNSKKND 722
++ELG++ N AD++P DL G RK + CL+ ++ +W +KER+ N K D
Sbjct: 223 LMELGSAPNLADFYPIFSGLDLQGLRKKMSKCLKEMFDIWEIFIKERRERHARNGVVKED 282
Query: 723 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVG 902
F+++ + +G DD+QIN L+ E+F AGT+T+ +T+EWA++EL KN E + EL +
Sbjct: 283 FLDVFISNGFDDEQINWLIHELFSAGTDTTTTTVEWAMAELLKNKEAMNKVCQELEREIN 342
Query: 903 KRPVKESDIPNMPYLQAFVKEXXXXXXXXXXXXXXXXXETCKVLNYTIPKECQIMVNAWG 1082
K + ES + PYL A +KE ETC+V+NYTIPK+ QI+VN W
Sbjct: 343 KNSINESQVSQFPYLYACIKE-TFRLHPPAPFLGHQPLETCEVMNYTIPKDSQIIVNIWA 401
Query: 1083 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 1190
+GRDP W DPL F PERFL SS+D KG+DFEL+PF
Sbjct: 402 VGRDPLAWEDPLLFKPERFLESSLDLKGHDFELLPF 437