BLASTX nr result
ID: Papaver31_contig00040755
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver31_contig00040755
(734 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 62 5e-15
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 62 5e-15
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 58 5e-14
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 60 8e-13
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 51 2e-11
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 49 8e-11
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 48 5e-10
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 46 9e-10
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 46 9e-10
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 45 2e-09
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 61.6 bits (148), Expect(2) = 5e-15
Identities = 34/49 (69%), Positives = 39/49 (79%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN Q+T+NM LELLSVVG+R VKESDI ++ YLQAFVKETL RLH
Sbjct: 303 KNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETL-----RLH 346
Score = 47.0 bits (110), Expect(2) = 5e-15
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = -3
Query: 630 DQTERKLNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
D E L+ +D Q+INAL++E+FG GTETSA I EW L+ELTK
Sbjct: 260 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTI-----EWALSELTK 303
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 61.6 bits (148), Expect(2) = 5e-15
Identities = 34/49 (69%), Positives = 39/49 (79%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN Q+T+NM LELLSVVG+R VKESDI ++ YLQAFVKETL RLH
Sbjct: 303 KNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETL-----RLH 346
Score = 47.0 bits (110), Expect(2) = 5e-15
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = -3
Query: 630 DQTERKLNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
D E L+ +D Q+INAL++E+FG GTETSA I EW L+ELTK
Sbjct: 260 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTI-----EWALSELTK 303
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 58.2 bits (139), Expect(2) = 5e-14
Identities = 32/49 (65%), Positives = 38/49 (77%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN ++T+NM ELLSVVG+R VKESDI ++ YLQAFVKETL RLH
Sbjct: 308 KNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETL-----RLH 351
Score = 47.0 bits (110), Expect(2) = 5e-14
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = -3
Query: 630 DQTERKLNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
D E L+ +D Q+INAL++E+FG GTETSA I EW L+ELTK
Sbjct: 265 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTI-----EWALSELTK 308
>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX
gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
somniferum]
Length = 440
Score = 59.7 bits (143), Expect(2) = 8e-13
Identities = 33/49 (67%), Positives = 39/49 (79%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN Q+T+++ ELLSVVG+R VKESDIL+L YLQAFVKETL RLH
Sbjct: 310 KNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETL-----RLH 353
Score = 41.6 bits (96), Expect(2) = 8e-13
Identities = 21/43 (48%), Positives = 30/43 (69%)
Frame = -3
Query: 612 LNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
L+ +D Q+IN+L++++F GTET+A I EW LTELTK
Sbjct: 273 LDSGLDDQQINSLLLDMFSAGTETTASTI-----EWALTELTK 310
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 50.8 bits (120), Expect(2) = 2e-11
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = -3
Query: 612 LNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
LN +D QKINAL++ELFG GTETSA I EW +TELTK
Sbjct: 270 LNAGLDDQKINALLMELFGAGTETSASTI-----EWAITELTK 307
Score = 45.8 bits (107), Expect(2) = 2e-11
Identities = 27/49 (55%), Positives = 32/49 (65%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
K + S + LEL++VVG VKESD+ L YLQAFVKETL RLH
Sbjct: 307 KKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETL-----RLH 350
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 48.5 bits (114), Expect(2) = 8e-11
Identities = 26/43 (60%), Positives = 31/43 (72%)
Frame = -3
Query: 612 LNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
LN ++ QKINAL++ELFG GTETSA I EW +TELTK
Sbjct: 270 LNAGLEDQKINALLMELFGTGTETSASTI-----EWAITELTK 307
Score = 45.8 bits (107), Expect(2) = 8e-11
Identities = 27/49 (55%), Positives = 32/49 (65%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
K + S + LEL++VVG VKESD+ L YLQAFVKETL RLH
Sbjct: 307 KKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETL-----RLH 350
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 48.1 bits (113), Expect(2) = 5e-10
Identities = 26/45 (57%), Positives = 31/45 (68%)
Frame = -3
Query: 612 LNFHIDYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTKIL 478
LN +D QKINAL++ELFG GTET+A I EW +TELT L
Sbjct: 270 LNAGLDDQKINALLMELFGAGTETTASTI-----EWAITELTNNL 309
Score = 43.5 bits (101), Expect(2) = 5e-10
Identities = 25/48 (52%), Positives = 32/48 (66%)
Frame = -2
Query: 484 NSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
N ++ S + EL++VVG + VKES+I L YLQA VKETL RLH
Sbjct: 308 NLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETL-----RLH 350
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 45.8 bits (107), Expect(2) = 9e-10
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN ++T+ + EL +VVG R VKESD +L YL+A VKETL RLH
Sbjct: 310 KNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETL-----RLH 353
Score = 45.1 bits (105), Expect(2) = 9e-10
Identities = 25/38 (65%), Positives = 28/38 (73%)
Frame = -3
Query: 597 DYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
DYQ INAL++ELFG GTETSA I EW +TELTK
Sbjct: 279 DYQ-INALLMELFGAGTETSASTI-----EWAMTELTK 310
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 45.8 bits (107), Expect(2) = 9e-10
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN ++T+ + EL +VVG R VKESD +L YL+A VKETL RLH
Sbjct: 310 KNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETL-----RLH 353
Score = 45.1 bits (105), Expect(2) = 9e-10
Identities = 25/38 (65%), Positives = 28/38 (73%)
Frame = -3
Query: 597 DYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
DYQ INAL++ELFG GTETSA I EW +TELTK
Sbjct: 279 DYQ-INALLMELFGAGTETSASTI-----EWAMTELTK 310
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 45.1 bits (105), Expect(2) = 2e-09
Identities = 25/38 (65%), Positives = 28/38 (73%)
Frame = -3
Query: 597 DYQKINALIVELFGGGTETSAPIICN*NCEWVLTELTK 484
DYQ INAL++ELFG GTETSA I EW +TELTK
Sbjct: 278 DYQ-INALLMELFGAGTETSASTI-----EWAMTELTK 309
Score = 44.7 bits (104), Expect(2) = 2e-09
Identities = 25/49 (51%), Positives = 33/49 (67%)
Frame = -2
Query: 487 KNSQLTSNMGLELLSVVGRRMVKESDILSLSYLQAFVKETLHSFIHRLH 341
KN ++T+ + E+ +VVG R VKESD +L YL+A VKETL RLH
Sbjct: 309 KNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETL-----RLH 352