BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver31_contig00035129
         (708 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACO90226.1| putative berberine bridge enzyme [Papaver bractea...   393   e-107
gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver so...   385   e-104
sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName...   382   e-104
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]          304   4e-80
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]          302   1e-79
gb|ACO90228.1| berberine bridge enzyme-like protein [Papaver bra...   287   4e-75
gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica]     285   2e-74
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName...   283   6e-74
pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   282   2e-73
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ...   282   2e-73
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   282   2e-73
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp...   281   3e-73
pdb|4PZF|A Chain A, Berberine Bridge Enzyme G164a Variant, A Ret...   280   8e-73
pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a ...   278   2e-72
pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ...   278   3e-72
pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ...   278   3e-72
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]         265   2e-68
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp....   254   3e-65
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]             251   3e-64
ref|XP_010063741.1| PREDICTED: reticuline oxidase-like [Eucalypt...   228   3e-57

>gb|ACO90226.1| putative berberine bridge enzyme [Papaver bracteatum]
          Length = 259

 Score =  393 bits (1010), Expect = e-107
 Identities = 197/217 (90%), Positives = 197/217 (90%)
 Frame = -2

Query: 653 QTCVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSF 474
           QTCVRGGDVNDNLLLSCLNSH VHNFTTLSTDTNSEY KLLHASIQNPLFAKPTVSKPSF
Sbjct: 9   QTCVRGGDVNDNLLLSCLNSHDVHNFTTLSTDTNSEYLKLLHASIQNPLFAKPTVSKPSF 68

Query: 473 IVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFE 294
           IVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFE
Sbjct: 69  IVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFE 128

Query: 293 SETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADN 114
           SETAWVESGATLGELYYAIAQSTETLGFTAGWCPTV              SRKYGLAADN
Sbjct: 129 SETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADN 188

Query: 113 VVDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
           VVDAILIDSKGAILDREKMGEDVFWAIR    GVWGA
Sbjct: 189 VVDAILIDSKGAILDREKMGEDVFWAIRGGGGGVWGA 225


>gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver somniferum]
          Length = 535

 Score =  385 bits (990), Expect = e-104
 Identities = 195/234 (83%), Positives = 200/234 (85%)
 Frame = -2

Query: 704 MMCRSXXXXXXXXXXXLQTCVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHA 525
           MMCRS           LQTCVRGGDVNDNLL SCLNSHGVHNFTTLSTDTNS+YFKLLHA
Sbjct: 1   MMCRSLTLRFFLFFVLLQTCVRGGDVNDNLLSSCLNSHGVHNFTTLSTDTNSDYFKLLHA 60

Query: 524 SIQNPLFAKPTVSKPSFIVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTP 345
           SIQNPLFAKPTVSKPSFIV+PGSKEELS T+HCCTRESWTIRLRSGGHSYEGLSYTADTP
Sbjct: 61  SIQNPLFAKPTVSKPSFIVMPGSKEELSRTVHCCTRESWTIRLRSGGHSYEGLSYTADTP 120

Query: 344 FVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXX 165
           FVIVDMMNL+ ISID ESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTV       
Sbjct: 121 FVIVDMMNLNRISIDVESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHIS 180

Query: 164 XXXXXXXSRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
                  SRKYGLAADNVVDAILIDS G+ILDREKMGEDVFWAIR    GVWGA
Sbjct: 181 GGGFGMMSRKYGLAADNVVDAILIDSTGSILDREKMGEDVFWAIRGGGGGVWGA 234


>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
           bridge-forming enzyme; Short=BBE; AltName:
           Full=Tetrahydroprotoberberine synthase; Flags: Precursor
           gi|3282517|gb|AAC61839.1| berberine bridge enzyme
           [Papaver somniferum]
          Length = 535

 Score =  382 bits (982), Expect = e-104
 Identities = 193/234 (82%), Positives = 200/234 (85%)
 Frame = -2

Query: 704 MMCRSXXXXXXXXXXXLQTCVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHA 525
           MMCRS           LQTCVRGGDVNDNLL SCLNSHGVHNFTTLSTDTNS+YFKLLHA
Sbjct: 1   MMCRSLTLRFFLFIVLLQTCVRGGDVNDNLLSSCLNSHGVHNFTTLSTDTNSDYFKLLHA 60

Query: 524 SIQNPLFAKPTVSKPSFIVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTP 345
           S+QNPLFAKPTVSKPSFIV+PGSKEELSST+HCCTRESWTIRLRSGGHSYEGLSYTADTP
Sbjct: 61  SMQNPLFAKPTVSKPSFIVMPGSKEELSSTVHCCTRESWTIRLRSGGHSYEGLSYTADTP 120

Query: 344 FVIVDMMNLHGISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXX 165
           FVIVDMMNL+ ISID  SETAWVESGATLGELYYAIAQST+TLGFTAGWCPTV       
Sbjct: 121 FVIVDMMNLNRISIDVLSETAWVESGATLGELYYAIAQSTDTLGFTAGWCPTVGSGGHIS 180

Query: 164 XXXXXXXSRKYGLAADNVVDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
                  SRKYGLAADNVVDAILIDS GAILDREKMG+DVFWAIR    GVWGA
Sbjct: 181 GGGFGMMSRKYGLAADNVVDAILIDSNGAILDREKMGDDVFWAIRGGGGGVWGA 234


>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  304 bits (778), Expect = 4e-80
 Identities = 148/216 (68%), Positives = 172/216 (79%)
 Frame = -2

Query: 650 TCVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFI 471
           TC    D+     LSCL S+GVHN+TT S+D+NS+Y +L H SIQNPLF K T+SKPS I
Sbjct: 27  TCALSDDI-----LSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTISKPSLI 81

Query: 470 VIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFES 291
           V+PG+KEELS+T+ CCTR SWTIRLRSGGHSYEGLSYTADTPFV++D+MNL+ ISID +S
Sbjct: 82  VLPGNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISIDMDS 141

Query: 290 ETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNV 111
           ETAWVESGAT+GELYYAI++ST++ GFTAGWCPTV              SRKYGLAADNV
Sbjct: 142 ETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNV 201

Query: 110 VDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
            D ILIDS GAILDR+ MGEDVFWA+R    GVWGA
Sbjct: 202 EDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGA 237


>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  302 bits (774), Expect = 1e-79
 Identities = 149/216 (68%), Positives = 171/216 (79%)
 Frame = -2

Query: 650 TCVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFI 471
           TC    D+     LSCL S+GVHN+TT S+D+NS+Y +L H SIQNPLF K T+SKPS I
Sbjct: 32  TCALSDDI-----LSCLTSNGVHNYTTPSSDSNSDYLRLYHLSIQNPLFEKSTISKPSLI 86

Query: 470 VIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFES 291
           V+P +KEELS+T+ CCTR SWTIRLRSGGHSYEGLSYTADTPFV++D+MNL+ ISID +S
Sbjct: 87  VLPRNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISIDIDS 146

Query: 290 ETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNV 111
           ETAWVESGATLGELYYAI + T++LGFTAGWCPTV              SRKYGLAADNV
Sbjct: 147 ETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNV 206

Query: 110 VDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
            D ILIDSKGAILDR+ MGEDVFWA+R    GVWGA
Sbjct: 207 EDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGA 242


>gb|ACO90228.1| berberine bridge enzyme-like protein [Papaver bracteatum]
          Length = 245

 Score =  287 bits (735), Expect = 4e-75
 Identities = 146/217 (67%), Positives = 165/217 (76%), Gaps = 2/217 (0%)
 Frame = -2

Query: 647 CVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVS--KPSF 474
           C  GG  ND++L+SCL SHGV N+T  S+D NS+Y +L H +I NPLF K T    KPS 
Sbjct: 28  CALGG--NDDILISCLASHGVENYTKPSSDPNSDYDRLFHINILNPLFKKTTSERLKPSL 85

Query: 473 IVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFE 294
           IV+P SK+ELSSTI CCTR SWTI+LRSGGHSYEGLSY +DTPFV+VDMMNL  ISID  
Sbjct: 86  IVMPNSKQELSSTIKCCTRSSWTIKLRSGGHSYEGLSYISDTPFVLVDMMNLDRISIDLN 145

Query: 293 SETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADN 114
           SETAWVESGAT+G+LYYAI +ST++ GFTAGWCP V              SRKYGLAADN
Sbjct: 146 SETAWVESGATIGQLYYAIHESTDSYGFTAGWCPNVGVGGHLSGGGLGMLSRKYGLAADN 205

Query: 113 VVDAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
           VVDAILIDS GAILDR+ MGEDVFWAIR    G WGA
Sbjct: 206 VVDAILIDSNGAILDRQSMGEDVFWAIRGGGGGCWGA 242


>gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica]
          Length = 242

 Score =  285 bits (730), Expect = 2e-74
 Identities = 143/215 (66%), Positives = 165/215 (76%)
 Frame = -2

Query: 647 CVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIV 468
           C  GG    N LLSCL  +GV N T  S D++S++ +LLH SIQNPLF    +SKPS I+
Sbjct: 20  CALGG----NDLLSCLTFNGVRNHTVFSADSDSDFNRLLHLSIQNPLFQNSLISKPSAII 75

Query: 467 IPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESE 288
           +PGSKEELS+TI C  + SWTIRLRSGGHSYEGLSYT+DTPF++VD+MNL+ +SID ESE
Sbjct: 76  LPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILVDLMNLNRVSIDLESE 135

Query: 287 TAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVV 108
           TAWVESG+TLGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVV
Sbjct: 136 TAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVV 195

Query: 107 DAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
           DAILID+ GAILDR+ MGEDVFWAIR    GVWGA
Sbjct: 196 DAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGA 230


>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
           bridge-forming enzyme; Short=BBE; AltName:
           Full=Tetrahydroprotoberberine synthase; Flags: Precursor
           gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
           oxidoreductase (methylene-bridge-forming) [Eschscholzia
           californica] gi|2897944|gb|AAC39358.1| berberine bridge
           enzyme [Eschscholzia californica]
          Length = 538

 Score =  283 bits (725), Expect = 6e-74
 Identities = 141/215 (65%), Positives = 164/215 (76%)
 Frame = -2

Query: 647 CVRGGDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIV 468
           C  GG    N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I+
Sbjct: 20  CALGG----NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAII 75

Query: 467 IPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESE 288
           +PGSKEELS+TI C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESE
Sbjct: 76  LPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESE 135

Query: 287 TAWVESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVV 108
           TAWVESG+TLGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVV
Sbjct: 136 TAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVV 195

Query: 107 DAILIDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
           DAILID+ GAILDR+ MGEDVFWAIR    GVWGA
Sbjct: 196 DAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGA 230


>pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
           (S)-Reticuline gi|210060956|pdb|3D2H|A Chain A,
           Structure Of Berberine Bridge Enzyme From Eschscholzia
           Californica, Monoclinic Crystal Form
           gi|210060957|pdb|3D2J|A Chain A, Structure Of Berberine
           Bridge Enzyme From Eschscholzia Californica, Tetragonal
           Crystal Form
          Length = 538

 Score =  282 bits (721), Expect = 2e-73
 Identities = 138/206 (66%), Positives = 161/206 (78%)
 Frame = -2

Query: 620 NLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS
Sbjct: 25  NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELS 84

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGAT 261
           +TI C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+T
Sbjct: 85  NTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGST 144

Query: 260 LGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKG 81
           LGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVVDAILID+ G
Sbjct: 145 LGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANG 204

Query: 80  AILDREKMGEDVFWAIRXXXXGVWGA 3
           AILDR+ MGEDVFWAIR    GVWGA
Sbjct: 205 AILDRQAMGEDVFWAIRGGGGGVWGA 230


>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
           Complex With (S)-Reticuline
          Length = 519

 Score =  282 bits (721), Expect = 2e-73
 Identities = 138/206 (66%), Positives = 161/206 (78%)
 Frame = -2

Query: 620 NLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS
Sbjct: 6   NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELS 65

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGAT 261
           +TI C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+T
Sbjct: 66  NTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGST 125

Query: 260 LGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKG 81
           LGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVVDAILID+ G
Sbjct: 126 LGELYYAITESSSKLGFTAGWCPTVGTGGAISGGGFGMMSRKYGLAADNVVDAILIDANG 185

Query: 80  AILDREKMGEDVFWAIRXXXXGVWGA 3
           AILDR+ MGEDVFWAIR    GVWGA
Sbjct: 186 AILDRQAMGEDVFWAIRGGGGGVWGA 211


>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
           Dehydroscoulerine
          Length = 519

 Score =  282 bits (721), Expect = 2e-73
 Identities = 138/206 (66%), Positives = 161/206 (78%)
 Frame = -2

Query: 620 NLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS
Sbjct: 6   NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELS 65

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGAT 261
           +TI C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+T
Sbjct: 66  NTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGST 125

Query: 260 LGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKG 81
           LGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVVDAILID+ G
Sbjct: 126 LGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANG 185

Query: 80  AILDREKMGEDVFWAIRXXXXGVWGA 3
           AILDR+ MGEDVFWAIR    GVWGA
Sbjct: 186 AILDRQAMGEDVFWAIRGGGGGVWGA 211


>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
           (S)-Scoulerine
          Length = 495

 Score =  281 bits (719), Expect = 3e-73
 Identities = 137/204 (67%), Positives = 160/204 (78%)
 Frame = -2

Query: 614 LLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 435
           LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS+T
Sbjct: 2   LLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSNT 61

Query: 434 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLG 255
           I C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLG
Sbjct: 62  IRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLG 121

Query: 254 ELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKGAI 75
           ELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVVDAILID+ GAI
Sbjct: 122 ELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAI 181

Query: 74  LDREKMGEDVFWAIRXXXXGVWGA 3
           LDR+ MGEDVFWAIR    GVWGA
Sbjct: 182 LDRQAMGEDVFWAIRGGGGGVWGA 205


>pdb|4PZF|A Chain A, Berberine Bridge Enzyme G164a Variant, A Reticuline
           Dehydrogenase gi|635576898|pdb|4PZF|B Chain B, Berberine
           Bridge Enzyme G164a Variant, A Reticuline Dehydrogenase
           gi|635576899|pdb|4PZF|C Chain C, Berberine Bridge Enzyme
           G164a Variant, A Reticuline Dehydrogenase
           gi|635576900|pdb|4PZF|D Chain D, Berberine Bridge Enzyme
           G164a Variant, A Reticuline Dehydrogenase
          Length = 538

 Score =  280 bits (715), Expect = 8e-73
 Identities = 137/206 (66%), Positives = 160/206 (77%)
 Frame = -2

Query: 620 NLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS
Sbjct: 25  NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELS 84

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGAT 261
           +TI C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+T
Sbjct: 85  NTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGST 144

Query: 260 LGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKG 81
           LGELYYAI +S+  LGFTA WCPTV              SRKYGLAADNVVDAILID+ G
Sbjct: 145 LGELYYAITESSSKLGFTAAWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANG 204

Query: 80  AILDREKMGEDVFWAIRXXXXGVWGA 3
           AILDR+ MGEDVFWAIR    GVWGA
Sbjct: 205 AILDRQAMGEDVFWAIRGGGGGVWGA 230


>pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
          Length = 498

 Score =  278 bits (711), Expect = 2e-72
 Identities = 137/206 (66%), Positives = 160/206 (77%)
 Frame = -2

Query: 620 NLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           N LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS
Sbjct: 3   NDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELS 62

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGAT 261
           +TI C  + SWTIRLRSGG SYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+T
Sbjct: 63  NTIRCIRKGSWTIRLRSGGASYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGST 122

Query: 260 LGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKG 81
           LGELYYAI +S+  LGFTAGWCPTV              SRKYGLAADNVVDAILID+ G
Sbjct: 123 LGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANG 182

Query: 80  AILDREKMGEDVFWAIRXXXXGVWGA 3
           AILDR+ MGEDVFWAIR    GVWGA
Sbjct: 183 AILDRQAMGEDVFWAIRGGGGGVWGA 208


>pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
           Complex With (S)-Reticuline
          Length = 497

 Score =  278 bits (710), Expect = 3e-72
 Identities = 136/204 (66%), Positives = 159/204 (77%)
 Frame = -2

Query: 614 LLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 435
           LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS+T
Sbjct: 2   LLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSNT 61

Query: 434 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLG 255
           I C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLG
Sbjct: 62  IRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLG 121

Query: 254 ELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKGAI 75
           ELYYAI +S+  LGFTAGW PTV              SRKYGLAADNVVDAILID+ GAI
Sbjct: 122 ELYYAITESSSKLGFTAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAI 181

Query: 74  LDREKMGEDVFWAIRXXXXGVWGA 3
           LDR+ MGEDVFWAIR    GVWGA
Sbjct: 182 LDRQAMGEDVFWAIRGGGGGVWGA 205


>pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
          Length = 495

 Score =  278 bits (710), Expect = 3e-72
 Identities = 136/204 (66%), Positives = 159/204 (77%)
 Frame = -2

Query: 614 LLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 435
           LLSCL  +GV N T  S D++S++ + LH SIQNPLF    +SKPS I++PGSKEELS+T
Sbjct: 2   LLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSNT 61

Query: 434 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLG 255
           I C  + SWTIRLRSGGHSYEGLSYT+DTPF+++D+MNL+ +SID ESETAWVESG+TLG
Sbjct: 62  IRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLG 121

Query: 254 ELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKGAI 75
           ELYYAI +S+  LGFTAGW PTV              SRKYGLAADNVVDAILID+ GAI
Sbjct: 122 ELYYAITESSSKLGFTAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAI 181

Query: 74  LDREKMGEDVFWAIRXXXXGVWGA 3
           LDR+ MGEDVFWAIR    GVWGA
Sbjct: 182 LDRQAMGEDVFWAIRGGGGGVWGA 205


>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
          Length = 536

 Score =  265 bits (678), Expect = 2e-68
 Identities = 128/211 (60%), Positives = 163/211 (77%)
 Frame = -2

Query: 635 GDVNDNLLLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGS 456
           GDVN   L SCL S+GV NFT LST ++S+Y +LL+ S+QN +F +P   +PS I++P S
Sbjct: 19  GDVN---LSSCLTSNGVSNFTALSTSSDSDYHRLLYVSMQNQIFTRPKYPRPSMIILPQS 75

Query: 455 KEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWV 276
           KEEL++++ C  R  WTIRLRSGGHSYEGLSY ADTPFV++D+MNL+ ISID ES+TAWV
Sbjct: 76  KEELAASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLNRISIDLESKTAWV 135

Query: 275 ESGATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAIL 96
           ESGATLGE+Y AI+++++TLGF+ G+CPTV              SRKYGLAADNV+DA++
Sbjct: 136 ESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHISGGGFGMMSRKYGLAADNVIDALI 195

Query: 95  IDSKGAILDREKMGEDVFWAIRXXXXGVWGA 3
           +D+ GA+LDR  MGEDVFWAIR    GVWGA
Sbjct: 196 VDANGAVLDRSSMGEDVFWAIRGGGGGVWGA 226


>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
          Length = 535

 Score =  254 bits (650), Expect = 3e-65
 Identities = 123/208 (59%), Positives = 158/208 (75%), Gaps = 1/208 (0%)
 Frame = -2

Query: 623 DNLLLSCLNSHGVHNFTTLSTDTNSE-YFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEE 447
           D+ L SCL  HGVHN+TT  + +NS+ Y +LL+ S+QN +F + T  +P  I++P S ++
Sbjct: 20  DDDLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRSTFPQPRVIILPESMDQ 79

Query: 446 LSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESG 267
           L++ I CCTR SWTIRLRSGGHSYEGLS+ AD PFVI+D+MNL+GISID +++TAWVESG
Sbjct: 80  LANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLNGISIDLDTQTAWVESG 139

Query: 266 ATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDS 87
           ATLGE+Y+AI +S+ T+ F+AG+CPT               SRKYGLAADNVVDA+L+D+
Sbjct: 140 ATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAPGGFGMMSRKYGLAADNVVDALLVDA 199

Query: 86  KGAILDREKMGEDVFWAIRXXXXGVWGA 3
            G +LDRE MGEDVFWAIR    GVWGA
Sbjct: 200 NGVVLDRESMGEDVFWAIRGGGGGVWGA 227


>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
          Length = 533

 Score =  251 bits (641), Expect = 3e-64
 Identities = 124/203 (61%), Positives = 155/203 (76%)
 Frame = -2

Query: 614 LLSCLNSHGVHNFTTLSTDTNSEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 435
           L SCL  HGV N+TT    +NS+Y +LL+ S+QN +F + T  +PS I++P SK++L +T
Sbjct: 28  LPSCLILHGVSNYTTPFI-SNSDYDRLLYVSMQNQIFTRSTFPRPSVIILPESKDQLVNT 86

Query: 434 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLG 255
           I CC R SWTIRLRSGGHSYEGLS+TAD+PFVI+DMMNL+G+SID +++TAW ESGATLG
Sbjct: 87  ISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSIDLDTQTAWAESGATLG 146

Query: 254 ELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDSKGAI 75
           E+Y+AI  S++ LGF+AG+CPTV              SRKYGLAADNVVDAILI + GA+
Sbjct: 147 EIYHAIGVSSDVLGFSAGYCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILISANGAL 206

Query: 74  LDREKMGEDVFWAIRXXXXGVWG 6
            DR+ MGEDVFWAIR    GVWG
Sbjct: 207 YDRKSMGEDVFWAIRGGGGGVWG 229


>ref|XP_010063741.1| PREDICTED: reticuline oxidase-like [Eucalyptus grandis]
          Length = 551

 Score =  228 bits (581), Expect = 3e-57
 Identities = 114/207 (55%), Positives = 145/207 (70%), Gaps = 4/207 (1%)
 Frame = -2

Query: 614 LLSCLNSHGVHNFTTLSTDTN--SEYFKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELS 441
           + SCL  H V+NFTT     N  + Y++LL++SI N  F+ P V KP  I+ PGS E+L 
Sbjct: 33  IASCLTQHNVNNFTTFPATENESARYYELLNSSIHNLRFSGPAVPKPVAIIFPGSVEQLK 92

Query: 440 STIHCCTRESWTIRLRSGGHSYEGLSYTAD--TPFVIVDMMNLHGISIDFESETAWVESG 267
           S+I CC   +W IR+RSGGHSYEG S+ A    PFV+VDMMNL+G+ +D ++ TAWVE G
Sbjct: 93  SSIVCCREGAWEIRVRSGGHSYEGTSWVATDGAPFVVVDMMNLNGVRVDLDTMTAWVEGG 152

Query: 266 ATLGELYYAIAQSTETLGFTAGWCPTVXXXXXXXXXXXXXXSRKYGLAADNVVDAILIDS 87
           ATLGE YYAIA++++  GF+AG CPTV              SRKYGLAADNVVDA+L+DS
Sbjct: 153 ATLGETYYAIAEASDVHGFSAGSCPTVGVGGHISGGGFGLLSRKYGLAADNVVDALLVDS 212

Query: 86  KGAILDREKMGEDVFWAIRXXXXGVWG 6
           +G +LDR+ MGEDVFWAIR    G+WG
Sbjct: 213 RGRLLDRKAMGEDVFWAIRGGGGGLWG 239