BLASTX nr result
ID: Papaver31_contig00030818
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver31_contig00030818
(863 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 491 e-136
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 491 e-136
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 488 e-135
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 437 e-120
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 413 e-112
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 410 e-112
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 407 e-111
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 369 2e-99
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 362 3e-97
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 350 1e-93
gb|ACM44075.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver rh... 240 9e-61
gb|ACM44072.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver br... 239 3e-60
gb|ACM44071.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver so... 238 6e-60
gb|ACM44073.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver nu... 233 2e-58
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 202 3e-49
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 201 8e-49
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 200 1e-48
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 199 2e-48
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 196 1e-47
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 196 1e-47
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 491 bits (1265), Expect = e-136
Identities = 249/269 (92%), Positives = 255/269 (94%)
Frame = -1
Query: 809 SLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 630
SLVAVVITT LYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 629 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 450
KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 449 VCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 270
VCRTELFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN
Sbjct: 121 VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 269 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVW 90
IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGIY+VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 89 GAMLKERKIAKQHNNSKKNDFVEILLDSG 3
GAMLKERKIAKQHNNSKKNDFVEILLDSG
Sbjct: 241 GAMLKERKIAKQHNNSKKNDFVEILLDSG 269
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 491 bits (1265), Expect = e-136
Identities = 251/272 (92%), Positives = 255/272 (93%)
Frame = -1
Query: 818 VTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDL 639
VTVSLVAVVITT LYLIFRDSS PIVGNLLQLGEKPHSQFAELAKTYGDL
Sbjct: 3 VTVSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDL 62
Query: 638 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 459
FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK
Sbjct: 63 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 122
Query: 458 LRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIF 279
LRKVCR ELFTQKMIESQAEIRE KAMEMVE+LK+N G+EVKI EVVFGTLVNIFGNLIF
Sbjct: 123 LRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIF 182
Query: 278 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 99
SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY
Sbjct: 183 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242
Query: 98 NVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
+VWGAMLKERKIAK HNNSKKNDFVEILLDSG
Sbjct: 243 SVWGAMLKERKIAKLHNNSKKNDFVEILLDSG 274
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 488 bits (1256), Expect = e-135
Identities = 247/269 (91%), Positives = 254/269 (94%)
Frame = -1
Query: 809 SLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTL 630
SLVAVVITT LYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSL 60
Query: 629 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 450
KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK
Sbjct: 61 KLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRK 120
Query: 449 VCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 270
VCRT+LFTQKMIESQAE+RE KAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN
Sbjct: 121 VCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 180
Query: 269 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYNVW 90
IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG+FDLFGQ KDVADCLQGIY+VW
Sbjct: 181 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240
Query: 89 GAMLKERKIAKQHNNSKKNDFVEILLDSG 3
GAMLKE KIAKQHNNSKKNDFVEILLDSG
Sbjct: 241 GAMLKESKIAKQHNNSKKNDFVEILLDSG 269
>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX
gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
somniferum]
Length = 440
Score = 437 bits (1124), Expect = e-120
Identities = 217/276 (78%), Positives = 241/276 (87%), Gaps = 1/276 (0%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 648
MEIVTV+L+A+V TT LYLI R+SS PIVGNLLQLGEKPHSQFA+LA+TY
Sbjct: 1 MEIVTVALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETY 60
Query: 647 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 468
GDLFTLKLG++TVVVASTPLAASE+LK HDR L GRYVFQSFRVK HVENSIVW+ECNET
Sbjct: 61 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 120
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
WKKLRKVCRT+LFTQKMIE+QAE+RE K MEMV+YLKKN G EVKI EV+FGTLVN+FGN
Sbjct: 121 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 180
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
LIFSQ+IFKLGDESSGS+EMK+H+WRMLELGNS NP DY P LG DLFGQRKDVADCLQ
Sbjct: 181 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 240
Query: 107 GIYNVWGAMLKERKIAK-QHNNSKKNDFVEILLDSG 3
G+Y VWGAMLKER+IAK Q N KNDFV++LLDSG
Sbjct: 241 GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSG 276
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 413 bits (1061), Expect = e-112
Identities = 205/276 (74%), Positives = 236/276 (85%), Gaps = 1/276 (0%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 648
ME+VTV+L+AV+I+++LYL+F S PIVGNLLQLGEKPH+QFAELA+TY
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60
Query: 647 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 468
GD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ET
Sbjct: 61 GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
WK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI EV+FGTLVNIFGN
Sbjct: 121 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
LIFSQNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+
Sbjct: 181 LIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240
Query: 107 GIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSG 3
GIY +WGAML+ERK+AK+ + KNDFV++ LDSG
Sbjct: 241 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSG 276
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 410 bits (1054), Expect = e-112
Identities = 204/276 (73%), Positives = 235/276 (85%), Gaps = 1/276 (0%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 648
ME+VTV+L+AV+I+++LYL+F S PIVGNLLQLGEKPH+QFAELA+TY
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60
Query: 647 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 468
GD+FTLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ET
Sbjct: 61 GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
WK LRKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI EV+FGTLVNIFGN
Sbjct: 121 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
LIFSQNIF+LG +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+
Sbjct: 181 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240
Query: 107 GIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSG 3
GIY +WGAML+ERK+AK+ + KNDFV++ LDSG
Sbjct: 241 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSG 276
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 407 bits (1046), Expect = e-111
Identities = 203/272 (74%), Positives = 233/272 (85%), Gaps = 1/272 (0%)
Frame = -1
Query: 815 TVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLF 636
TV+L+AV+I+++LYL+F S PIVGNLLQLGEKPH+QFAELA+TYGD+F
Sbjct: 4 TVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIF 63
Query: 635 TLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKL 456
TLK+G+ETVVVAST AASEILKTHDR+LS RYVFQSFRVK HVENSIVWS+C ETWK L
Sbjct: 64 TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 123
Query: 455 RKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFS 276
RKVCRTELFTQKMIESQA +RE K EMVEYL K G EVKI EV+FGTLVNIFGNLIFS
Sbjct: 124 RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 183
Query: 275 QNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYN 96
QNIF+LGD +SGS E KE+LWRMLELGNSTNPADYFP LG+FDLFGQRK+VA+CL+GIY
Sbjct: 184 QNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 243
Query: 95 VWGAMLKERKIAKQHNNSK-KNDFVEILLDSG 3
+WGAML+ERK+AK+ + K KNDFV++ LDSG
Sbjct: 244 IWGAMLQERKLAKKVDGYKSKNDFVDVCLDSG 275
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 369 bits (946), Expect = 2e-99
Identities = 180/275 (65%), Positives = 225/275 (81%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 648
ME++++++V+ L+ I RDS PIVGNLLQLG+KPH++FA+LA+ Y
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 647 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 468
G+LF+LKLGS+TVVVAS+P AA+EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI EVVFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
LIFS+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +G+ DLFGQR+ VA+ LQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240
Query: 107 GIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
IY+VWG MLKER+ K + KNDFV++LL++G
Sbjct: 241 QIYDVWGVMLKERRGTK--GSESKNDFVDVLLNAG 273
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 362 bits (928), Expect = 3e-97
Identities = 178/275 (64%), Positives = 221/275 (80%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTY 648
ME+V++++V+ L+ I RD PIVGNLLQLG+KP ++FA+LA+ Y
Sbjct: 1 MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60
Query: 647 GDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNET 468
G LF+LKLGS+TVVVAS+P AA+E+LKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+
Sbjct: 61 GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
WK LRKVCRTELFT KMIESQ+EIRE KA EMV++L+ G VKI EVVFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
LIFS+++F L D + GSVE+KEHLW++LE+GNSTNPADYFP +G++DLFGQR+ VAD LQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240
Query: 107 GIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
IY+VWG LKER+ K + NDFV++LL++G
Sbjct: 241 QIYDVWGINLKERRGMK--GSESNNDFVDVLLNAG 273
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 350 bits (897), Expect = 1e-93
Identities = 174/276 (63%), Positives = 219/276 (79%), Gaps = 1/276 (0%)
Frame = -1
Query: 827 MEIVTVSLVAVVITTL-LYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKT 651
ME+++ ++V++ L ++ I DS PIVGNLLQLGEKPH++FA+LA+
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 650 YGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNE 471
YG+LFTLKLGS+TVVVAS+P AA+EILKT D++LSGRYVFQSFRV EHV NSIVWSECNE
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 470 TWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 291
WK LRKVCRTELF+ KMIESQA IRE KA++MV +L+K EVKI EVVF TLVNIFG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 290 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 111
NLIFS+++F L D + GS E+KEHLW++L++GNSTNPADYFP +G+FDLFGQR++VA L
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 110 QGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
+ IY+VWG MLKER+ H ++NDF +LL++G
Sbjct: 241 KQIYDVWGVMLKERRSITGH---RENDFANVLLNAG 273
>gb|ACM44075.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver rhoeas]
Length = 139
Score = 240 bits (613), Expect = 9e-61
Identities = 124/139 (89%), Positives = 126/139 (90%)
Frame = -1
Query: 794 VITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 615
VITT LYLIFRDSS PIVGNLLQLGEKPHSQFAELA+TYGDLFTLKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAQTYGDLFTLKLGSE 60
Query: 614 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 435
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 120
Query: 434 LFTQKMIESQAEIRECKAM 378
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44072.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
gi|222101959|gb|ACM44074.1| (S)-N-methylcoclaurine
3'-hydroxylase [Papaver orientale]
Length = 139
Score = 239 bits (609), Expect = 3e-60
Identities = 123/139 (88%), Positives = 125/139 (89%)
Frame = -1
Query: 794 VITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 615
VITT LYLIFRDSS PIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 60
Query: 614 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 435
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN+TWKKLRKVCR E
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNDTWKKLRKVCRAE 120
Query: 434 LFTQKMIESQAEIRECKAM 378
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44071.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 139
Score = 238 bits (606), Expect = 6e-60
Identities = 122/139 (87%), Positives = 126/139 (90%)
Frame = -1
Query: 794 VITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 615
VITT LYLIFRDSS PIVGNLLQLGEKPHSQFA+LA+TYGDLF+LKLGSE
Sbjct: 1 VITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSE 60
Query: 614 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 435
TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 120
Query: 434 LFTQKMIESQAEIRECKAM 378
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>gb|ACM44073.1| (S)-N-methylcoclaurine 3'-hydroxylase [Papaver nudicaule]
Length = 139
Score = 233 bits (593), Expect = 2e-58
Identities = 119/139 (85%), Positives = 123/139 (88%)
Frame = -1
Query: 794 VITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSE 615
VITT LY IFRDS+ PIVGNLLQLGEKPHSQFA+LA+TYGDLFTLKLGSE
Sbjct: 1 VITTFLYFIFRDSTPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFTLKLGSE 60
Query: 614 TVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTE 435
TVVVASTPLAASEILK HDRVLSGRYVFQSFRVK HVENSIVWSECNETWKKLRKVCRTE
Sbjct: 61 TVVVASTPLAASEILKAHDRVLSGRYVFQSFRVKNHVENSIVWSECNETWKKLRKVCRTE 120
Query: 434 LFTQKMIESQAEIRECKAM 378
LFTQKMIESQAE+RE KAM
Sbjct: 121 LFTQKMIESQAEVRESKAM 139
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 202 bits (514), Expect = 3e-49
Identities = 106/271 (39%), Positives = 175/271 (64%), Gaps = 1/271 (0%)
Frame = -1
Query: 812 VSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLL-QLGEKPHSQFAELAKTYGDLF 636
VS + +++ L ++F S PI+G LL +L ++PH + ++LA+ +G L
Sbjct: 5 VSFIFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRFGPLM 64
Query: 635 TLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKL 456
LK G E VVVAS+ +AA E+LK DR+LSGR+ S R+K ++E+S+VW++C + WK +
Sbjct: 65 LLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMV 124
Query: 455 RKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFS 276
RKV RTELF+ KM++ QA RE K E++++L + G +V A+V+FG+++NI G LIFS
Sbjct: 125 RKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFS 184
Query: 275 QNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYN 96
++++ D + ++ MK + +++ L N AD +P LG D G RK A C++ +
Sbjct: 185 KDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMNE 244
Query: 95 VWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
W A++K+R +++++ KNDF+++LLDSG
Sbjct: 245 SWAAIVKQR---RKNDDHSKNDFLQVLLDSG 272
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 201 bits (510), Expect = 8e-49
Identities = 105/275 (38%), Positives = 176/275 (64%), Gaps = 7/275 (2%)
Frame = -1
Query: 806 LVAVVITTLLYLIFR----DSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGDL 639
L+ + + L++ IF+ S P++GN+ Q+G+ PH AKTYG L
Sbjct: 10 LLPLFLFPLIFFIFKIFKKSSKITQLPPGPTPWPVIGNIFQIGKMPHITLTNFAKTYGPL 69
Query: 638 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVK--EHVENSIVW-SECNET 468
+L+LG++ +VV S+P A EI KTHDR+LSGR+V + R K + + S+ W SEC+
Sbjct: 70 ISLRLGTQCLVVGSSPSVAIEIFKTHDRILSGRHVPNAVRSKGSDLEKTSLGWKSECDSG 129
Query: 467 WKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 288
W+ LR +CRT+LF K++ESQA +RE K ME+VE+L+ G V I E+VF T++N+ N
Sbjct: 130 WRYLRTLCRTDLFAGKVLESQACLREKKVMELVEFLRSKEGQVVNIGELVFATVLNMLSN 189
Query: 287 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQ 108
++ S+++ L +E+ S E+K + ++E+G++ N +D++P LG+ DL G RK D +
Sbjct: 190 VLISKDMIHLEEETEDS-EIKSLVRGIVEVGSAPNISDFYPILGKLDLQGLRKKSTDLVT 248
Query: 107 GIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDSG 3
++W +++ER+ ++++++S + DF+E LLD+G
Sbjct: 249 KFCSIWEPIIEERRNSRENDSSSQQDFLETLLDNG 283
>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 200 bits (508), Expect = 1e-48
Identities = 102/241 (42%), Positives = 160/241 (66%), Gaps = 3/241 (1%)
Frame = -1
Query: 716 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 537
I+GN+L G+KPH A AKT+G L +LKLG + ++V S+ AA+EILK+HDR+LS RY
Sbjct: 42 IIGNILHFGKKPHISTANFAKTHGPLISLKLGKQLLIVGSSKRAATEILKSHDRLLSARY 101
Query: 536 VFQSFRVKEHVEN--SIVW-SECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 366
VF++ + HV + SIVW ++C++ WK LR +CR ELF+ K IESQA +RE K EMVE
Sbjct: 102 VFKAALFENHVLDRISIVWATQCSDGWKSLRALCRNELFSAKAIESQAVLREKKMGEMVE 161
Query: 365 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 186
++ + G V I EVV + N NL+FS ++ L D+ + + +K +WRM++LG +
Sbjct: 162 FIGRRKGEVVGIGEVVLAIVFNTIANLLFSVDLIGLEDDGA-ATGLKSLMWRMMKLGATP 220
Query: 185 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 6
N +D++P LG D G ++ +A C+ ++ +WG +KER+ K ++ ++DF+++ L +
Sbjct: 221 NISDFYPILGGIDPQGLKRKMAVCVNQMFGIWGKYIKERR-EKHVHDGPRSDFLDVFLAN 279
Query: 5 G 3
G
Sbjct: 280 G 280
>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223549945|gb|EEF51432.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 199 bits (506), Expect = 2e-48
Identities = 105/277 (37%), Positives = 169/277 (61%), Gaps = 4/277 (1%)
Frame = -1
Query: 821 IVTVSLVAVVITTLLYLIFRDSSXXXXXXXXXXXPIVGNLLQLGEKPHSQFAELAKTYGD 642
++ + ++ V++T ++ I PI+GN+LQ+ +K H A AK YG
Sbjct: 10 LLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFAKVYGP 69
Query: 641 LFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN--SIVWSE-CNE 471
L +L+LG++ +VVASTP +A+E+LK HDR+LS R++ ++ + H+ +IVW+ CN+
Sbjct: 70 LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129
Query: 470 TWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFG 291
WK LR +CRTELF+ K IESQA +RE K EM+E+L G V +AEVVFGT+ N
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189
Query: 290 NLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCL 111
+L+FS ++ ++ G +K LW M+E+ S N A+++P L D G ++ + CL
Sbjct: 190 HLLFSTDLIGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCL 246
Query: 110 QGIYNVWGAMLKERKIAKQHNN-SKKNDFVEILLDSG 3
+ ++ VW +KER+ H++ + K DF++I L +G
Sbjct: 247 KEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNG 283
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 196 bits (499), Expect = 1e-47
Identities = 105/241 (43%), Positives = 151/241 (62%), Gaps = 3/241 (1%)
Frame = -1
Query: 716 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 537
I+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 587 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 646
Query: 536 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 366
+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE
Sbjct: 647 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 706
Query: 365 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 186
+L G VK+ ++VF T+ NI N FS ++ DE +K+ + + E G +
Sbjct: 707 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 766
Query: 185 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 6
N +DY+P LG D+ G + + + I W +LKER+ ++ N S DF+E LL+
Sbjct: 767 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 825
Query: 5 G 3
G
Sbjct: 826 G 826
Score = 188 bits (477), Expect = 5e-45
Identities = 104/240 (43%), Positives = 145/240 (60%), Gaps = 4/240 (1%)
Frame = -1
Query: 716 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 537
I+GNLLQLG+ PH + A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 1092 IIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRY 1151
Query: 536 VFQSFRVKEHVENSIVWS---ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 366
V S VK+ N + + EC WK LR +CRTE+F+ K +ES E+RE K ME+VE
Sbjct: 1152 VSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVE 1211
Query: 365 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 186
+L G VK+ ++VF T+ NI N FS ++ E +K+ ++R ELG +
Sbjct: 1212 FLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELGATP 1266
Query: 185 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHN-NSKKNDFVEILLD 9
N +D++P L +L G +K + L I W LKER+ KQ N S D +E L+
Sbjct: 1267 NLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLE 1324
Score = 177 bits (449), Expect = 9e-42
Identities = 99/241 (41%), Positives = 146/241 (60%), Gaps = 3/241 (1%)
Frame = -1
Query: 716 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 537
I+GNLLQ+G H + A LAK +G L +L+LG++ +VVAS+ AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGRY 106
Query: 536 VFQSFRVKEHVENSIVWS---ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 366
V + V N + + CN W+ L+ +CR ELF+ K +ES+ E+RE K ME+VE
Sbjct: 107 VSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVE 166
Query: 365 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 186
+L+K G VK+ ++V+ T+ NI N FS + V +K+ L ELG +T
Sbjct: 167 FLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELG-AT 225
Query: 185 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 6
N D++P LG D+ G RK + + + I W +LKER+ + H +S DF++ LL++
Sbjct: 226 NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSS-HGDFLDALLET 284
Query: 5 G 3
G
Sbjct: 285 G 285
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 196 bits (499), Expect = 1e-47
Identities = 105/241 (43%), Positives = 151/241 (62%), Gaps = 3/241 (1%)
Frame = -1
Query: 716 IVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRY 537
I+GNLLQ+G+ PH++ A LAK +G L +L+LG++ +VVAS+P AA E+LKTHDR LSGRY
Sbjct: 47 IIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRY 106
Query: 536 VFQSFRVKEHVEN--SIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEIRECKAMEMVE 366
+ S VK N SIV++ +CN WK LR +CR ELF+ K +ESQ E+RE K E+VE
Sbjct: 107 LSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVE 166
Query: 365 YLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNST 186
+L G VK+ ++VF T+ NI N FS ++ DE +K+ + + E G +
Sbjct: 167 FLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATP 226
Query: 185 NPADYFPFLGRFDLFGQRKDVADCLQGIYNVWGAMLKERKIAKQHNNSKKNDFVEILLDS 6
N +DY+P LG D+ G + + + I W +LKER+ ++ N S DF+E LL+
Sbjct: 227 NLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR-TQRSNRSSHRDFLEALLEI 285
Query: 5 G 3
G
Sbjct: 286 G 286