BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver31_contig00021446
         (1074 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   527   e-147
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   526   e-147
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   523   e-145
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   492   e-136
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   479   e-132
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   478   e-132
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   476   e-131
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   425   e-116
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   419   e-114
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   408   e-111
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   279   3e-72
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   274   8e-71
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              271   7e-70
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   271   7e-70
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   271   9e-70
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   268   6e-69
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]      266   2e-68
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   263   1e-67
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   261   5e-67
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   261   9e-67

>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  527 bits (1358), Expect = e-147
 Identities = 259/312 (83%), Positives = 290/312 (92%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPHSQFA+LAKTYGD+FTLKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 35  WPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSG 94

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCR ELF+QKMI+SQAE RE KAMEMVE+
Sbjct: 95  RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEF 154

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           LK+N+G+EVKI+EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 155 LKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 214

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFPFLG  DL GQRK+VADCLQGIY VWG +LKER+IA K   N KKNDFV++LLD+
Sbjct: 215 PADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIA-KLHNNSKKNDFVEILLDS 273

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D+QINALLMEIFG GTETS STIEWALSELTKNPEVTANMRSELLSVVGKRP+KESD
Sbjct: 274 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESD 333

Query: 38  IPNMPYLQAFVK 3
           IPNMPYLQAFVK
Sbjct: 334 IPNMPYLQAFVK 345


>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1; AltName:
           Full=Cytochrome P450 80B3
           gi|6176562|gb|AAF05621.1|AF191772_1
           (S)-N-methylcoclaurine 3'-hydroxylase [Papaver
           somniferum]
          Length = 481

 Score =  526 bits (1356), Expect = e-147
 Identities = 260/312 (83%), Positives = 288/312 (92%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPHSQFAQLA+TYGD+F+LKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 30  WPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSG 89

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCRTELF+QKMI+SQAE RE KAMEMVEY
Sbjct: 90  RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEY 149

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           LKKN GNEVKI EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 150 LKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 209

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFPFLG  DL GQ K+VADCLQGIY VWG +LKER+IA KQ  N KKNDFV++LLD+
Sbjct: 210 PADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIA-KQHNNSKKNDFVEILLDS 268

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D+QINALLMEIFG GTETS STIEWALSELTKNP+VTANMR ELLSVVGKRP+KESD
Sbjct: 269 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESD 328

Query: 38  IPNMPYLQAFVK 3
           IPNMPYLQAFVK
Sbjct: 329 IPNMPYLQAFVK 340


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  523 bits (1347), Expect = e-145
 Identities = 258/312 (82%), Positives = 287/312 (91%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPHSQFAQLA+TYGD+F+LKLG+ETVVVASTPLAASEILKTHDR+LSG
Sbjct: 30  WPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSG 89

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+ HV+ S+ WS+CNETW KLRKVCRT+LF+QKMI+SQAE RE KAMEMVEY
Sbjct: 90  RYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAMEMVEY 149

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           LKKN GNEVKI EV+FGTLVNIFGNLIFSQNIF+LGDE+SGSV+MKE +WRMLELGN+TN
Sbjct: 150 LKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTN 209

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFPFLG  DL GQ K+VADCLQGIY VWG +LKE +IA KQ  N KKNDFV++LLD+
Sbjct: 210 PADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIA-KQHNNSKKNDFVEILLDS 268

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D+QINALLMEIFG GTETS STIEWALSELTKNP+VTANMR ELLSVVGKRP+KESD
Sbjct: 269 GLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESD 328

Query: 38  IPNMPYLQAFVK 3
           IPNMPYLQAFVK
Sbjct: 329 IPNMPYLQAFVK 340


>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
           AltName: Full=Cytochrome P450 80BX
           gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
           somniferum]
          Length = 440

 Score =  492 bits (1266), Expect = e-136
 Identities = 238/312 (76%), Positives = 280/312 (89%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPHSQFAQLA+TYGD+FTLKLGT+TVVVASTPLAASE+LK HDR L G
Sbjct: 36  WPIVGNLLQLGEKPHSQFAQLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCG 95

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+ HV+ S+ W++CNETW KLRKVCRT+LF+QKMI++QAE RE K MEMV+Y
Sbjct: 96  RYVFQSFRVKNHVENSIVWNECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKY 155

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           LKKNEG EVKI+EVIFGTLVN+FGNLIFSQ+IF+LGDE+SGS++MK+ IWRMLELGN+ N
Sbjct: 156 LKKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSAN 215

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           P DY P LGSLDL GQRK+VADCLQG+Y VWG +LKERRIA +Q     KNDFVDVLLD+
Sbjct: 216 PGDYVPLLGSLDLFGQRKDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDS 275

Query: 215 GLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D+QIN+LL+++F  GTET+ STIEWAL+ELTKNP+VTA++RSELLSVVGKR +KESD
Sbjct: 276 GLDDQQINSLLLDMFSAGTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESD 335

Query: 38  IPNMPYLQAFVK 3
           I N+PYLQAFVK
Sbjct: 336 ILNLPYLQAFVK 347


>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
           californica [Expression vector pWCD2353]
          Length = 490

 Score =  479 bits (1232), Expect = e-132
 Identities = 234/312 (75%), Positives = 269/312 (86%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST  AASEILKTHDRILS 
Sbjct: 36  WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 95

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+GHV+ S+ WSDC ETW  LRKVCRTELF+QKMI+SQA  RE K  EMVEY
Sbjct: 96  RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 155

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELGD NSGS + KE +WRMLELGN+TN
Sbjct: 156 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTN 215

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFP LG  DL GQRKEVA+CL+GIY +WG +L+ER++A K D    KNDFVDV LD+
Sbjct: 216 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDS 275

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D QINALLME+FG GTETS STIEWA++ELTKNP++TA +RSEL +VVG+R +KESD
Sbjct: 276 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESD 335

Query: 38  IPNMPYLQAFVK 3
            PN+PYL+A VK
Sbjct: 336 FPNLPYLEATVK 347


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  478 bits (1229), Expect = e-132
 Identities = 233/312 (74%), Positives = 269/312 (86%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST  AASEILKTHDRILS 
Sbjct: 35  WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 94

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+GHV+ S+ WSDC ETW  LRKVCRTELF+QKMI+SQA  RE K  EMVEY
Sbjct: 95  RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 154

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELGD NSGS + KE +WRMLELGN+TN
Sbjct: 155 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTN 214

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFP LG  DL GQRKEVA+CL+GIY +WG +L+ER++A K D    KNDFVDV LD+
Sbjct: 215 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDS 274

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D QINALLME+FG GTETS STIEWA++ELTKNP++TA +RSE+ +VVG+R +KESD
Sbjct: 275 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESD 334

Query: 38  IPNMPYLQAFVK 3
            PN+PYL+A VK
Sbjct: 335 FPNLPYLEATVK 346


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  476 bits (1225), Expect = e-131
 Identities = 233/312 (74%), Positives = 268/312 (85%), Gaps = 1/312 (0%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPIVGNLLQL +KPH+QFA+LA+TYGDIFTLK+GTETVVVAST  AASEILKTHDRILS 
Sbjct: 36  WPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSA 95

Query: 755 RYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEY 576
           RYV+QSFRV+GHV+ S+ WSDC ETW  LRKVCRTELF+QKMI+SQA  RE K  EMVEY
Sbjct: 96  RYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEY 155

Query: 575 LKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTN 396
           L K +G EVKI+EVIFGTLVNIFGNLIFSQNIFELG  NSGS + KE +WRMLELGN+TN
Sbjct: 156 LMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTN 215

Query: 395 PADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDT 216
           PADYFP LG  DL GQRKEVA+CL+GIY +WG +L+ER++A K D    KNDFVDV LD+
Sbjct: 216 PADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDS 275

Query: 215 GLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESD 39
           GL D QINALLME+FG GTETS STIEWA++ELTKNP++TA +RSEL +VVG+R +KESD
Sbjct: 276 GLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESD 335

Query: 38  IPNMPYLQAFVK 3
            PN+PYL+A VK
Sbjct: 336 FPNLPYLEATVK 347


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
           japonica] gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  425 bits (1093), Expect = e-116
 Identities = 205/311 (65%), Positives = 262/311 (84%), Gaps = 1/311 (0%)
 Frame = -2

Query: 932 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 753
           PIVGNLLQL DKPH++FA+LA+ YG++F+LKLG++TVVVAS+P AA+EILKTHD+ILSGR
Sbjct: 37  PIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGR 96

Query: 752 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 573
           YV+QSFRV+ HV+ S+ WS+CN+ W  LRKVCRTELF+ KMI+SQ+E RE KA EMV++L
Sbjct: 97  YVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFL 156

Query: 572 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 393
           +  EG  VKI+EV+FGTLVNIFGNLIFS+++F+L D   GSV++KE +W++L++GN+TNP
Sbjct: 157 RGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNP 216

Query: 392 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 213
           ADYFP +G LDL GQR+ VA+ LQ IYDVWGV+LKERR     +    KNDFVDVLL+ G
Sbjct: 217 ADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSE---SKNDFVDVLLNAG 273

Query: 212 LGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESDI 36
           L D++INALLME+FG GTETS STIEWA++ELTK P V + +R EL++VVG   +KESD+
Sbjct: 274 LDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDL 333

Query: 35  PNMPYLQAFVK 3
           P++PYLQAFVK
Sbjct: 334 PHLPYLQAFVK 344


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  419 bits (1077), Expect = e-114
 Identities = 203/311 (65%), Positives = 258/311 (82%), Gaps = 1/311 (0%)
 Frame = -2

Query: 932 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 753
           PIVGNLLQL DKP ++FA+LA+ YG +F+LKLG++TVVVAS+P AA+E+LKTHD+ILSGR
Sbjct: 37  PIVGNLLQLGDKPRAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGR 96

Query: 752 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 573
           YV+QSFRV+ HV+ S+ WS+CN+ W  LRKVCRTELF+ KMI+SQ+E RE KA EMV++L
Sbjct: 97  YVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFL 156

Query: 572 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 393
           +  EG  VKI+EV+FGTLVNIFGNLIFS+++F+L D   GSV++KE +W++LE+GN+TNP
Sbjct: 157 RSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNP 216

Query: 392 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 213
           ADYFP +G  DL GQR+ VAD LQ IYDVWG+ LKERR     + N   NDFVDVLL+ G
Sbjct: 217 ADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESN---NDFVDVLLNAG 273

Query: 212 LGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESDI 36
           L D++INALLME+FG GTETS STIEWA++ELTK P V + +R EL++VVG   +KESD+
Sbjct: 274 LEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDL 333

Query: 35  PNMPYLQAFVK 3
           P++PYLQAFVK
Sbjct: 334 PHLPYLQAFVK 344


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
           glaucum]
          Length = 491

 Score =  408 bits (1048), Expect = e-111
 Identities = 197/311 (63%), Positives = 255/311 (81%), Gaps = 1/311 (0%)
 Frame = -2

Query: 932 PIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSGR 753
           PIVGNLLQL +KPH++FA+LA+ YG++FTLKLG++TVVVAS+P AA+EILKT D+ILSGR
Sbjct: 38  PIVGNLLQLGEKPHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGR 97

Query: 752 YVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVEYL 573
           YV+QSFRV  HV  S+ WS+CNE W  LRKVCRTELFS KMI+SQA  RE KA++MV +L
Sbjct: 98  YVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFL 157

Query: 572 KKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTTNP 393
           +K E  EVKI+EV+F TLVNIFGNLIFS+++F+L D   GS ++KE +W++L++GN+TNP
Sbjct: 158 RKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNP 217

Query: 392 ADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLDTG 213
           ADYFP +G  DL GQR+EVA  L+ IYDVWGV+LKERR         ++NDF +VLL+ G
Sbjct: 218 ADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGH----RENDFANVLLNAG 273

Query: 212 LGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIKESDI 36
           L D++INALLME+FG GTET+ STIEWA++ELT N  V + +R+EL++VVG + +KES+I
Sbjct: 274 LDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVGHKTVKESNI 333

Query: 35  PNMPYLQAFVK 3
           P++PYLQA VK
Sbjct: 334 PHLPYLQAIVK 344


>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nelumbo nucifera]
          Length = 485

 Score =  279 bits (713), Expect = 3e-72
 Identities = 144/314 (45%), Positives = 212/314 (67%), Gaps = 3/314 (0%)
 Frame = -2

Query: 935 WPIVGNLL-QLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILS 759
           WPI+G LL +L  +PH + ++LA+ +G +  LK G E VVVAS+ +AA E+LK  DR+LS
Sbjct: 35  WPIIGTLLPKLKKQPHVELSKLAQRFGPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLS 94

Query: 758 GRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVE 579
           GR+   S R++G+++ SM W+DC + W  +RKV RTELFS KM+  QA ARE K  E+++
Sbjct: 95  GRFAPHSVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQAHAREEKVSELMK 154

Query: 578 YLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTT 399
           +L + EG +V   +VIFG+++NI G LIFS+++++  D    ++ MK  I +++ L    
Sbjct: 155 FLIRKEGEKVNFADVIFGSILNILGALIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIP 214

Query: 398 NPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLLD 219
           N AD +P LG  D  G RK  A C++ + + W  ++K+RR   K D +  KNDF+ VLLD
Sbjct: 215 NLADLYPILGGSDFQGLRKASAACVKRMNESWAAIVKQRR---KNDDH-SKNDFLQVLLD 270

Query: 218 TGLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGK-RPIKE 45
           +G  D QI+A+L+E FG G++TS STIEWA++EL +NPE    +R EL  V+ +   +KE
Sbjct: 271 SGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKE 330

Query: 44  SDIPNMPYLQAFVK 3
           SD+PN+PYL A VK
Sbjct: 331 SDLPNLPYLHACVK 344


>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  274 bits (701), Expect = 8e-71
 Identities = 145/316 (45%), Positives = 206/316 (65%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935 WPIVGNLLQL---SDKP-HSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDR 768
           WP++GNL  L   ++ P H   A +A+T+G I  L LGT+  V+AST  AA EILKTHDR
Sbjct: 35  WPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDR 94

Query: 767 ILSGRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
           I S R++  SFR++ H+K S+ WSDC + W  LRK+ RTE+FS KM+Q+Q+  RE K  E
Sbjct: 95  IFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAE 154

Query: 587 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
           ++++L+  EG  VKI + +FGTL+NI GN++FS+++F  GDE      ++  I  ML +G
Sbjct: 155 LIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKG-GIQNLIREMLMIG 213

Query: 407 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
              N A+++P L  LDL G +K+  +    +  +W   +KER    K ++N +  D +DV
Sbjct: 214 AEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKER----KANRNEESKDMLDV 269

Query: 227 LLDTGLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
           LL     D QINAL +E FG G+ETS +TIEW ++EL K+P+  A +R EL  VVG   I
Sbjct: 270 LLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTI 329

Query: 50  KESDIPNMPYLQAFVK 3
           KESD+P +PYLQA +K
Sbjct: 330 KESDLPQLPYLQACIK 345


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  271 bits (693), Expect = 7e-70
 Identities = 137/316 (43%), Positives = 199/316 (62%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935  WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
            WPI+GNLLQ+   PH++ A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 585  WPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSG 644

Query: 755  RYVYQSFRVEG----HVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
            RY+  S  V+     H+ +  A  DCN  W  LR +CR ELFS K ++SQ E RE K  E
Sbjct: 645  RYLSSSVPVKNPKLNHLSIVFA-KDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTE 703

Query: 587  MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
            +VE+L   EG  VK+++++F T+ NI  N  FS ++ +  DE      +K+ I +  E G
Sbjct: 704  LVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFG 763

Query: 407  NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
             T N +DY+P LG LD+ G  ++  +  + I   W  +LKERR   ++       DF++ 
Sbjct: 764  ATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEA 821

Query: 227  LLDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
            LL+ G  D QIN +++E+F  G ETS  T+EWA++EL +N +    +R EL  +VG+ P+
Sbjct: 822  LLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPV 881

Query: 50   KESDIPNMPYLQAFVK 3
            +ES +P +PYLQA VK
Sbjct: 882  RESHLPRLPYLQACVK 897



 Score =  261 bits (668), Expect = 5e-67
 Identities = 135/316 (42%), Positives = 200/316 (63%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935  WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
            WPI+GNLLQL   PH + A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 1090 WPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSG 1149

Query: 755  RYVYQSFRVE----GHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
            RYV  S  V+     H+ ++ A  +C   W  LR +CRTE+FS K ++S  E RE K ME
Sbjct: 1150 RYVSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVME 1208

Query: 587  MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
            +VE+L   EG  VK+++++F T+ NI  N  FS ++ +   E      +K+ I+R  ELG
Sbjct: 1209 LVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELG 1263

Query: 407  NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
             T N +D++P L  L+L G +K+  + L  I   W   LKERR   +++      D ++ 
Sbjct: 1264 ATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEA 1321

Query: 227  LLDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
             L+    D QIN +++E+F  G +TS  TIEWA+++L +NP+V   +R EL  ++G+ P+
Sbjct: 1322 FLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPV 1381

Query: 50   KESDIPNMPYLQAFVK 3
            +ES +P++PYLQA VK
Sbjct: 1382 RESHLPHLPYLQACVK 1397



 Score =  254 bits (650), Expect = 6e-65
 Identities = 135/316 (42%), Positives = 200/316 (63%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GNLLQ+    H + A LAK +G + +L+LGT+ +VVAS+  AA E+LKTHDR LSG
Sbjct: 45  WPIIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSG 104

Query: 755 RYVYQSFRVE----GHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
           RYV  +  V      H+ ++ A   CN  W  L+ +CR ELFS K ++S+ E RE K ME
Sbjct: 105 RYVSTTIPVNSPKLNHLAMAFA-KVCNSDWRNLKAICRMELFSGKAMESRVELRERKVME 163

Query: 587 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
           +VE+L+K EG  VK++++++ T+ NI  N  FS +  +    +   V +K+ +    ELG
Sbjct: 164 LVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDLLNENAELG 223

Query: 407 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
             TN  D++P LG LD+ G RK++ +  + I   W  +LKERR   ++       DF+D 
Sbjct: 224 -ATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDFLDA 280

Query: 227 LLDTGLGDRQINALLMEI-FGGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
           LL+TG  D QIN ++ME+ F G ETS  T+EWA++EL KN +    + +EL  ++G+ P+
Sbjct: 281 LLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPV 340

Query: 50  KESDIPNMPYLQAFVK 3
           +ES +P++PYLQA VK
Sbjct: 341 RESHLPHLPYLQACVK 356


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  271 bits (693), Expect = 7e-70
 Identities = 137/316 (43%), Positives = 199/316 (62%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GNLLQ+   PH++ A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45  WPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSG 104

Query: 755 RYVYQSFRVEG----HVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
           RY+  S  V+     H+ +  A  DCN  W  LR +CR ELFS K ++SQ E RE K  E
Sbjct: 105 RYLSSSVPVKNPKLNHLSIVFA-KDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTE 163

Query: 587 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
           +VE+L   EG  VK+++++F T+ NI  N  FS ++ +  DE      +K+ I +  E G
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFG 223

Query: 407 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
            T N +DY+P LG LD+ G  ++  +  + I   W  +LKERR   ++       DF++ 
Sbjct: 224 ATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEA 281

Query: 227 LLDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
           LL+ G  D QIN +++E+F  G ETS  T+EWA++EL +N +    +R EL  +VG+ P+
Sbjct: 282 LLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPV 341

Query: 50  KESDIPNMPYLQAFVK 3
           +ES +P +PYLQA VK
Sbjct: 342 RESHLPRLPYLQACVK 357


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  271 bits (692), Expect = 9e-70
 Identities = 137/316 (43%), Positives = 199/316 (62%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GNLLQ+   PH++ A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45  WPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSG 104

Query: 755 RYVYQSFRVEG----HVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
           RY+  S  V+     H+ +  A  DCN  W  LR +CR ELFS K ++SQ E RE K  E
Sbjct: 105 RYLSXSVPVKNPKLNHLSIVFA-KDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTE 163

Query: 587 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
           +VE+L   EG  VK+++++F T+ NI  N  FS ++ +  DE      +K+ I +  E G
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFG 223

Query: 407 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
            T N +DY+P LG LD+ G  ++  +  + I   W  +LKERR   ++       DF++ 
Sbjct: 224 ATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERR--TQRSNRSSHRDFLEA 281

Query: 227 LLDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
           LL+ G  D QIN +++E+F  G ETS  T+EWA++EL +N +    +R EL  +VG+ P+
Sbjct: 282 LLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPV 341

Query: 50  KESDIPNMPYLQAFVK 3
           +ES +P +PYLQA VK
Sbjct: 342 RESHLPRLPYLQACVK 357


>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223549945|gb|EEF51432.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  268 bits (685), Expect = 6e-69
 Identities = 132/315 (41%), Positives = 201/315 (63%), Gaps = 4/315 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GN+LQ+  K H   A  AK YG + +L+LG + +VVASTP +A+E+LK HDR+LS 
Sbjct: 43  WPILGNILQVDKKLHICIANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSA 102

Query: 755 RYVYQSFRVEGHV--KVSMAWSD-CNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 585
           R++ ++   E H+  +V++ W+  CN+ W  LR +CRTELFS K I+SQA  RE K  EM
Sbjct: 103 RFILKAIPSESHILERVAIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEM 162

Query: 584 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 405
           +E+L   +G  V + EV+FGT+ N   +L+FS ++    ++  G   +K  +W M+E+  
Sbjct: 163 LEFLITKQGQAVNVAEVVFGTIFNTISHLLFSTDLIGFENQIGG---VKSLLWSMMEMAT 219

Query: 404 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 225
           + N A+++P L  LD  G ++++  CL+ ++ VW + +KERR  +  D    K DF+D+ 
Sbjct: 220 SPNIAEFYPILAPLDPQGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIF 279

Query: 224 LDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIK 48
           L  G  D QIN L+ME+   GT+T+ +++EWA++EL KN E    +R EL   + K PIK
Sbjct: 280 LSNGFDDDQINWLVMELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIK 339

Query: 47  ESDIPNMPYLQAFVK 3
           ES +  +PYL A VK
Sbjct: 340 ESHVSQLPYLNACVK 354


>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
          Length = 483

 Score =  266 bits (680), Expect = 2e-68
 Identities = 143/315 (45%), Positives = 196/315 (62%), Gaps = 4/315 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKP---HSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRI 765
           WPI+GN+  L       H     +A+T+G I  L LGT+   VAST  AA EILKTHDR 
Sbjct: 34  WPIIGNIPALFSSAYPMHVALTNMARTHGPIMILWLGTQPTAVASTTEAAMEILKTHDRS 93

Query: 764 LSGRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 585
            SGR V  SFR++ H+K S+ W+DC+E W  LRK+ RTE+FS KM+Q Q   RE K  E+
Sbjct: 94  FSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAEL 153

Query: 584 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 405
           VE+LK  EG  VKI +++FGTL+NI GN+IFS+++F     +     M+  I  ML +G 
Sbjct: 154 VEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGDK--MQTLIKEMLMIGA 211

Query: 404 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 225
             N A+++P     DL G +K        +  +W   ++ER++     +N +  D +DVL
Sbjct: 212 APNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKV----KRNEEPKDMLDVL 267

Query: 224 LDTGLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIK 48
           L     D QINA+ +E FG G+ETS +T EWAL+EL KNP + A +R EL  VVGK  +K
Sbjct: 268 LLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVK 327

Query: 47  ESDIPNMPYLQAFVK 3
           ESD+P++PYLQA +K
Sbjct: 328 ESDLPHLPYLQACIK 342


>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Nelumbo nucifera]
          Length = 486

 Score =  263 bits (673), Expect = 1e-67
 Identities = 135/315 (42%), Positives = 204/315 (64%), Gaps = 4/315 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSD-KPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILS 759
           WPI+G  L     KP+ +  +LA+ YG +   + G E VVVAS  +AA E+LK  DR+LS
Sbjct: 35  WPIIGTQLPSPTMKPNLELFKLAQRYGPLMLFRFGFENVVVASNHVAAMEVLKNQDRVLS 94

Query: 758 GRYVYQSFRVEGHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEMVE 579
           GR+   S RV+G+++ SM W+DC + W  +RK+ RTELFS KM+   A  RE K  E+++
Sbjct: 95  GRFKANSVRVKGYIEYSMVWADCTDYWKMVRKILRTELFSTKMLDVHAHVREEKVSELMK 154

Query: 578 YLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGNTT 399
           +L++ EG EV  ++VIFG ++N+ G LI+S+++++  D+   ++ MK  I +++ L  T 
Sbjct: 155 FLRRKEGEEVNFVDVIFGCILNMLGALIYSKDVYDFEDKTDINLGMKGMIRQLMILAATP 214

Query: 398 NPADYFP-FLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVLL 222
           N AD +P F    D  G RKE A C++ + + W  ++ ERR    ++ +  KND + VLL
Sbjct: 215 NIADLYPIFFDGSDFQGLRKESAACVKRMSESWAAIINERR----KNNDHTKNDLLQVLL 270

Query: 221 DTGLGDRQINALLMEIFG-GTETSGSTIEWALSELTKNPEVTANMRSELLSVVGK-RPIK 48
           D+G  D QI+A+ +E FG G++TS STIEWAL+EL +NPE    +  EL  V+G+   +K
Sbjct: 271 DSGFSDPQIDAIFLETFGPGSDTSASTIEWALAELLRNPEKLVKLHEELDRVIGRNNTVK 330

Query: 47  ESDIPNMPYLQAFVK 3
           +SD+PN+PYL A VK
Sbjct: 331 DSDLPNLPYLHACVK 345


>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 493

 Score =  261 bits (668), Expect = 5e-67
 Identities = 135/316 (42%), Positives = 200/316 (63%), Gaps = 5/316 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GNLLQL   PH + A LAK +G + +L+LGT+ +VVAS+P AA E+LKTHDR LSG
Sbjct: 45  WPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSG 104

Query: 755 RYVYQSFRVE----GHVKVSMAWSDCNETWHKLRKVCRTELFSQKMIQSQAEARECKAME 588
           RYV  S  V+     H+ ++ A  +C   W  LR +CRTE+FS K ++S  E RE K ME
Sbjct: 105 RYVSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSGKAMESHVELRERKVME 163

Query: 587 MVEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELG 408
           +VE+L   EG  VK+++++F T+ NI  N  FS ++ +   E      +K+ I+R  ELG
Sbjct: 164 LVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFEREG-----LKDFIYRAAELG 218

Query: 407 NTTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDV 228
            T N +D++P L  L+L G +K+  + L  I   W   LKERR   +++      D ++ 
Sbjct: 219 ATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEA 276

Query: 227 LLDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPI 51
            L+    D QIN +++E+F  G +TS  TIEWA+++L +NP+V   +R EL  ++G+ P+
Sbjct: 277 FLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPV 336

Query: 50  KESDIPNMPYLQAFVK 3
           +ES +P++PYLQA VK
Sbjct: 337 RESHLPHLPYLQACVK 352


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547255|gb|EEF48750.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  261 bits (666), Expect = 9e-67
 Identities = 136/315 (43%), Positives = 203/315 (64%), Gaps = 4/315 (1%)
 Frame = -2

Query: 935 WPIVGNLLQLSDKPHSQFAQLAKTYGDIFTLKLGTETVVVASTPLAASEILKTHDRILSG 756
           WPI+GN+LQ+   PH   A  AK +G + +L+LGT  VVVAS+P AA+EILKTHDR+LSG
Sbjct: 44  WPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSG 103

Query: 755 RYVYQSFRVEGHV--KVSMAWS-DCNETWHKLRKVCRTELFSQKMIQSQAEARECKAMEM 585
           RY+  +   E +V  ++++ W+  C++ W  LR +CR+ELFS K I+SQA  RE K  EM
Sbjct: 104 RYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEM 163

Query: 584 VEYLKKNEGNEVKIIEVIFGTLVNIFGNLIFSQNIFELGDENSGSVDMKERIWRMLELGN 405
           +++L   +G  V I EV+F T  N   NL+FS+++    D+ +   ++K  I  ++EL  
Sbjct: 164 LDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAG-ELKTLISTLMELAT 222

Query: 404 TTNPADYFPFLGSLDLLGQRKEVADCLQGIYDVWGVVLKERRIANKQDKNIKKNDFVDVL 225
             N AD++P L  LD  G ++++ +CL+ ++ VW + +KERR   +  K+ +K DF+DV 
Sbjct: 223 CPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERR--ERHVKDARKTDFLDVF 280

Query: 224 LDTGLGDRQINALLMEIF-GGTETSGSTIEWALSELTKNPEVTANMRSELLSVVGKRPIK 48
           L  G  D QIN LL+E+F  G +T+ +T+EWA++EL K   V   +R EL + +GK  I+
Sbjct: 281 LSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIR 340

Query: 47  ESDIPNMPYLQAFVK 3
           ES IP + YL A VK
Sbjct: 341 ESHIPQLKYLNACVK 355