BLASTX nr result
ID: Papaver31_contig00019770
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver31_contig00019770
(1644 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246... 121 2e-24
gb|AFB74616.1| cytochrome P450 [Papaver somniferum] 108 1e-20
gb|AKO60176.1| 1,2-dehydroreticuline synthase [Papaver rhoeas] g... 72 2e-09
sp|L7X3S1.1|MSH_PAPSO RecName: Full=Methyltetrahydroprotoberberi... 68 2e-08
gb|AFB74617.1| cytochrome P450 [Papaver somniferum] 67 4e-08
gb|AKO60179.1| reticuline epimerase [Papaver bracteatum] 63 7e-07
gb|AKO60180.1| reticuline epimerase [Papaver somniferum subsp. s... 63 9e-07
gb|AKO60175.1| reticuline epimerase [Papaver somniferum] gi|8572... 63 9e-07
sp|P0DKI7.1|STORR_PAPSO RecName: Full=Bifunctional protein STORR... 63 9e-07
ref|XP_002284810.3| PREDICTED: cytochrome P450 CYP82D47 [Vitis v... 63 9e-07
emb|CBI32510.3| unnamed protein product [Vitis vinifera] 63 9e-07
emb|CAN64371.1| hypothetical protein VITISV_016844 [Vitis vinifera] 63 9e-07
ref|XP_013458973.1| cytochrome P450 family 82 protein [Medicago ... 62 2e-06
ref|XP_010028533.1| PREDICTED: cytochrome P450 CYP82D47-like [Eu... 62 2e-06
ref|XP_010451089.1| PREDICTED: cytochrome P450 82C4 [Camelina sa... 60 4e-06
ref|XP_010028535.1| PREDICTED: cytochrome P450 CYP82D47-like [Eu... 60 4e-06
ref|XP_010445016.1| PREDICTED: cytochrome P450 82C4-like [Cameli... 60 6e-06
ref|XP_010918630.1| PREDICTED: cytochrome P450 82C4-like [Elaeis... 59 1e-05
>gb|AFB74614.1| cytochrome P450 [Papaver somniferum] gi|388571246|gb|AFK73719.1|
cytochrome P450 [Papaver somniferum]
Length = 540
Score = 121 bits (303), Expect = 2e-24
Identities = 68/102 (66%), Positives = 83/102 (81%)
Frame = -3
Query: 1624 GDTASIWEKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGC 1445
GDT S E+YKKT+ EA + L S FAVSD+FP LEWVDR RGL+R++KR DELNSI GC
Sbjct: 224 GDTESKGERYKKTLEEA-LHLMSIFAVSDIFPSLEWVDRLRGLIRNMKRFGDELNSIAGC 282
Query: 1444 LIEEHRRKRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
LIEEHR+KR L ++SK +DKGV D+ QDF+DVLLS+AE+SQ
Sbjct: 283 LIEEHRQKR-LQSVSK--SDKGVGDE-QDFVDVLLSVAEKSQ 320
>gb|AFB74616.1| cytochrome P450 [Papaver somniferum]
Length = 554
Score = 108 bits (270), Expect = 1e-20
Identities = 58/97 (59%), Positives = 73/97 (75%), Gaps = 2/97 (2%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
E+YKKT+ E FVVL + FAVSD+FP LE++DR RGLV+D+K DELNSI GC IEEHR+
Sbjct: 239 ERYKKTLDEMFVVLATRFAVSDIFPSLEFIDRLRGLVKDMKILGDELNSIAGCFIEEHRQ 298
Query: 1423 KR--SLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
KR SL++L N+ D QDFIDVLLS+ +QS+
Sbjct: 299 KRRESLSSLLSLSNES--VGDEQDFIDVLLSIMDQSR 333
>gb|AKO60176.1| 1,2-dehydroreticuline synthase [Papaver rhoeas]
gi|857282524|gb|AKO60182.1| 1,2-dehydroreticuline
synthase [Papaver rhoeas]
Length = 535
Score = 71.6 bits (174), Expect = 2e-09
Identities = 33/83 (39%), Positives = 52/83 (62%)
Frame = -3
Query: 1549 AVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRKRSLATLSKHGNDKGVSD 1370
AVSD FP L W+DR GL++D+K ++L+ + +IE+HR+KR + +K G++ D
Sbjct: 235 AVSDTFPWLGWIDRLTGLIKDMKHHAEKLDLVVESIIEDHRQKRRFSR-TKKGDENNTED 293
Query: 1369 DPQDFIDVLLSLAEQSQKRHQGY 1301
+ DFID+ LS+ EQ + Y
Sbjct: 294 EQDDFIDICLSIMEQPELPGNNY 316
>sp|L7X3S1.1|MSH_PAPSO RecName: Full=Methyltetrahydroprotoberberine 14-monooxygenase;
AltName: Full=(S)-cis-N-methylstylopine 14-hydroxylase;
AltName:
Full=(S)-cis-N-methyltetrahydroprotoberberine-14-
hydroxylase; AltName: Full=Methyltetrahydroprotoberberine
14-hydroxylase; AltName: Full=N-methylstylopine
hydroxylase; Short=MSH [Papaver somniferum]
gi|443429262|gb|AGC92398.1| (S)-cis-N-methylstylopine
14-hydroxylase [Papaver somniferum]
Length = 526
Score = 68.2 bits (165), Expect = 2e-08
Identities = 41/94 (43%), Positives = 52/94 (55%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
++YK M EA + L FA SDV P L W+D RGLVR++K+ E++SI +EEHR
Sbjct: 223 QRYKAAMDEA-MRLMRFFAFSDVIPSLSWLDNLRGLVREMKKCASEIDSIMATWVEEHRV 281
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQS 1322
KR N G S DFIDV L + E S
Sbjct: 282 KR---------NSGGNSQLEHDFIDVCLDIMEHS 306
>gb|AFB74617.1| cytochrome P450 [Papaver somniferum]
Length = 556
Score = 67.4 bits (163), Expect = 4e-08
Identities = 37/95 (38%), Positives = 58/95 (61%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
EKYK + E ++ ++FAVSDV P L W+DR GL +K +L+++ G +E+HR+
Sbjct: 242 EKYKLAIDEVSNLM-ATFAVSDVVPRLGWIDRLTGLTGKMKNCGKKLDAVVGDAVEDHRQ 300
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
K+ +S++ + +DFIDV LS+ EQSQ
Sbjct: 301 KK--LKISRNNTGALTEHEEEDFIDVCLSIMEQSQ 333
>gb|AKO60179.1| reticuline epimerase [Papaver bracteatum]
Length = 889
Score = 63.2 bits (152), Expect = 7e-07
Identities = 35/95 (36%), Positives = 60/95 (63%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
E++K+ + EA +++S VSD P L W+D+ GL R++K +L+ + +I++HR+
Sbjct: 239 EQFKEVINEASYFMSTS-PVSDNVPMLGWIDQLTGLTRNMKHCGKKLDLVVESIIKDHRQ 297
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
KR + +K G++K D+ DFID+ LS+ EQ Q
Sbjct: 298 KRRFSR-TKGGDEK--DDEQDDFIDICLSIMEQPQ 329
>gb|AKO60180.1| reticuline epimerase [Papaver somniferum subsp. setigerum]
Length = 900
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/95 (36%), Positives = 59/95 (62%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
E++K+ + EA +++S VSD P L W+D+ GL R++K +L+ + +I +HR+
Sbjct: 250 EQFKEAINEASYFMSTS-PVSDNVPMLGWIDQLTGLTRNMKHCGKKLDLVVESIINDHRQ 308
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
KR + +K G++K D+ DFID+ LS+ EQ Q
Sbjct: 309 KRRFSR-TKGGDEK--DDEQDDFIDICLSIMEQPQ 340
>gb|AKO60175.1| reticuline epimerase [Papaver somniferum] gi|857282396|gb|AKO60181.1|
reticuline epimerase [Papaver somniferum]
Length = 900
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/95 (36%), Positives = 59/95 (62%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
E++K+ + EA +++S VSD P L W+D+ GL R++K +L+ + +I +HR+
Sbjct: 250 EQFKEAINEASYFMSTS-PVSDNVPMLGWIDQLTGLTRNMKHCGKKLDLVVESIINDHRQ 308
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
KR + +K G++K D+ DFID+ LS+ EQ Q
Sbjct: 309 KRRFSR-TKGGDEK--DDEQDDFIDICLSIMEQPQ 340
>sp|P0DKI7.1|STORR_PAPSO RecName: Full=Bifunctional protein STORR; AltName: Full=(S)- to
(R)-reticuline; Includes: RecName: Full=Cytochrome P450
82Y2; AltName: Full=CYP82Y2; Includes: RecName:
Full=Oxydoreductase gi|852381174|gb|AKN63431.1| STORR
[Papaver somniferum]
Length = 901
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/95 (36%), Positives = 59/95 (62%)
Frame = -3
Query: 1603 EKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRR 1424
E++K+ + EA +++S VSD P L W+D+ GL R++K +L+ + +I +HR+
Sbjct: 251 EQFKEAINEASYFMSTS-PVSDNVPMLGWIDQLTGLTRNMKHCGKKLDLVVESIINDHRQ 309
Query: 1423 KRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
KR + +K G++K D+ DFID+ LS+ EQ Q
Sbjct: 310 KRRFSR-TKGGDEK--DDEQDDFIDICLSIMEQPQ 341
>ref|XP_002284810.3| PREDICTED: cytochrome P450 CYP82D47 [Vitis vinifera]
Length = 610
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/94 (37%), Positives = 49/94 (52%)
Frame = -3
Query: 1600 KYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRK 1421
K K E F++L F VSDV P L W+D +G ++KR E++ + G +EEHR+
Sbjct: 300 KRAKKAFEDFIILLGLFMVSDVIPFLGWLDTMKGFTAEMKRVAKEVDYVLGSWVEEHRQN 359
Query: 1420 RSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
R A ND G QDFI +LS+ + Q
Sbjct: 360 RLSA------NDNGAE---QDFIHAMLSVIDDGQ 384
>emb|CBI32510.3| unnamed protein product [Vitis vinifera]
Length = 607
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/94 (37%), Positives = 49/94 (52%)
Frame = -3
Query: 1600 KYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRK 1421
K K E F++L F VSDV P L W+D +G ++KR E++ + G +EEHR+
Sbjct: 285 KRAKKAFEDFIILLGLFMVSDVIPFLGWLDTMKGFTAEMKRVAKEVDYVLGSWVEEHRQN 344
Query: 1420 RSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
R A ND G QDFI +LS+ + Q
Sbjct: 345 RLSA------NDNGAE---QDFIHAMLSVIDDGQ 369
>emb|CAN64371.1| hypothetical protein VITISV_016844 [Vitis vinifera]
Length = 527
Score = 62.8 bits (151), Expect = 9e-07
Identities = 35/94 (37%), Positives = 49/94 (52%)
Frame = -3
Query: 1600 KYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRK 1421
K K E F++L F VSDV P L W+D +G ++KR E++ + G +EEHR+
Sbjct: 217 KRXKKAFEDFIILLGLFMVSDVIPFLGWLDTMKGFTAEMKRVAKEVDYVLGSWVEEHRQN 276
Query: 1420 RSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
R A ND G QDFI +LS+ + Q
Sbjct: 277 RLSA------NDNGAE---QDFIHAMLSVIDDGQ 301
>ref|XP_013458973.1| cytochrome P450 family 82 protein [Medicago truncatula]
gi|657391913|gb|KEH33015.1| cytochrome P450 family 82
protein [Medicago truncatula]
Length = 532
Score = 62.0 bits (149), Expect = 2e-06
Identities = 41/103 (39%), Positives = 59/103 (57%), Gaps = 3/103 (2%)
Frame = -3
Query: 1627 FGDTASIWEKYKKTMVEAF---VVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNS 1457
FG T I ++ + V+A + L F V D P L+W D G V+ +K+A E++
Sbjct: 210 FGSTNVIDKEEVEICVKAIKEMLRLMGVFTVGDALPFLKWFDFG-GHVKAMKKATKEMDK 268
Query: 1456 ITGCLIEEHRRKRSLATLSKHGNDKGVSDDPQDFIDVLLSLAE 1328
I G L+EEH+RKRSL+ + K V D QDF+DV+LSL +
Sbjct: 269 IIGDLLEEHQRKRSLS------DQKEVDGDHQDFMDVMLSLLD 305
>ref|XP_010028533.1| PREDICTED: cytochrome P450 CYP82D47-like [Eucalyptus grandis]
gi|629089035|gb|KCW55288.1| hypothetical protein
EUGRSUZ_I01215 [Eucalyptus grandis]
Length = 524
Score = 62.0 bits (149), Expect = 2e-06
Identities = 34/88 (38%), Positives = 48/88 (54%)
Frame = -3
Query: 1573 FVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRKRSLATLSKH 1394
F L F VSD P L W+D RG V ++KR EL+ + G +EEHR++R++A
Sbjct: 226 FFHLVGEFMVSDAIPFLGWLDVLRGRVAEMKRTAKELDWVLGRWVEEHRKRRAVAK---- 281
Query: 1393 GNDKGVSDDPQDFIDVLLSLAEQSQKRH 1310
V DD QDFI +LS E + ++
Sbjct: 282 -----VEDDEQDFIHFMLSATELEEGKY 304
>ref|XP_010451089.1| PREDICTED: cytochrome P450 82C4 [Camelina sativa]
Length = 524
Score = 60.5 bits (145), Expect = 4e-06
Identities = 36/97 (37%), Positives = 51/97 (52%)
Frame = -3
Query: 1591 KTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRKRSL 1412
K + F L F VSD FP L W D +G +++K+ EL+ I IE HR++R +
Sbjct: 219 KIAISKFFHLIGIFTVSDAFPALRWFDL-QGHEKEMKQTGSELDVILEKWIENHRQQRKV 277
Query: 1411 ATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQKRHQGY 1301
+ G ++ DFIDV+LSLAEQ + H Y
Sbjct: 278 S---------GTKENDSDFIDVMLSLAEQGKLSHLQY 305
>ref|XP_010028535.1| PREDICTED: cytochrome P450 CYP82D47-like [Eucalyptus grandis]
Length = 526
Score = 60.5 bits (145), Expect = 4e-06
Identities = 33/88 (37%), Positives = 48/88 (54%)
Frame = -3
Query: 1573 FVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRKRSLATLSKH 1394
F L F VSD P L W+D +G V ++KR EL+ + G +EEHR++R++A
Sbjct: 228 FSHLVGEFMVSDAIPFLGWLDIVKGRVAEMKRTAKELDWVLGRWVEEHRKRRAVAK---- 283
Query: 1393 GNDKGVSDDPQDFIDVLLSLAEQSQKRH 1310
V DD QDFI +LS E + ++
Sbjct: 284 -----VEDDEQDFIHFMLSATELEEGKY 306
>ref|XP_010445016.1| PREDICTED: cytochrome P450 82C4-like [Camelina sativa]
Length = 524
Score = 60.1 bits (144), Expect = 6e-06
Identities = 36/97 (37%), Positives = 51/97 (52%)
Frame = -3
Query: 1591 KTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELNSITGCLIEEHRRKRSL 1412
K + F L F VSD FP L W D +G +++K+ EL+ I IE HR++R +
Sbjct: 219 KRAISKFFHLIGIFTVSDAFPSLRWFDL-QGHEKEMKQTGSELDVILERWIENHRQERKV 277
Query: 1411 ATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQKRHQGY 1301
+ G ++ DFIDV+LSLAEQ + H Y
Sbjct: 278 S---------GTKENDSDFIDVMLSLAEQGKLSHLQY 305
>ref|XP_010918630.1| PREDICTED: cytochrome P450 82C4-like [Elaeis guineensis]
Length = 493
Score = 59.3 bits (142), Expect = 1e-05
Identities = 35/107 (32%), Positives = 58/107 (54%)
Frame = -3
Query: 1639 KYMNFGDTASIWEKYKKTMVEAFVVLTSSFAVSDVFPCLEWVDRSRGLVRDLKRARDELN 1460
+Y G + +++K + F +L +F SD+FPCL+W+D RG +K E++
Sbjct: 175 RYFGSGTGSDEAWRFRKAISRLFGLL-DAFVASDMFPCLKWMD-WRGHESAMKNTAQEVD 232
Query: 1459 SITGCLIEEHRRKRSLATLSKHGNDKGVSDDPQDFIDVLLSLAEQSQ 1319
SI L+EEHR++R + PQDF+DV+LS+ E ++
Sbjct: 233 SIMAGLLEEHRQRRLSGK---------ATGGPQDFMDVMLSIVEDAE 270