BLASTX nr result
ID: Papaver31_contig00009263
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver31_contig00009263
(1564 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AKO60155.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 420 e-114
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum] 416 e-113
gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum] 416 e-113
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 286 5e-74
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 281 1e-72
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353] 278 1e-71
sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 278 1e-71
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 276 4e-71
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 276 5e-71
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 275 1e-70
gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Pap... 274 1e-70
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 274 2e-70
sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 274 2e-70
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 273 3e-70
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 272 5e-70
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 271 2e-69
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 271 2e-69
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 266 5e-68
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 265 1e-67
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ... 263 4e-67
>gb|AKO60155.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 356
Score = 420 bits (1079), Expect = e-114
Identities = 210/359 (58%), Positives = 272/359 (75%), Gaps = 2/359 (0%)
Frame = -3
Query: 1499 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSG-KSMLTLTELSNS 1323
MEIQM D+E EMK Q +WN +CGSV++SVLKC+I LG+ D I+ S K M+TL+ELS++
Sbjct: 1 MEIQMGDQEQEMKNQGLVWNKICGSVDSSVLKCTIELGLLDTINSSSSKPMITLSELSST 60
Query: 1322 LSFSNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGI 1143
+ IKT NLYRLLRYL+HMNLI I+S + + FSLTDL+KLLL QE+SLKDW +GI
Sbjct: 61 SPSLHSIKTQNLYRLLRYLTHMNLITINSTEATESFSLTDLAKLLLKTQERSLKDWVLGI 120
Query: 1142 GDVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAM 963
D + V WHE+SS C T + +HGK +EL E PE +E + M+ DT + M
Sbjct: 121 DDEHSVNGWHELSSYCCSPDDAPTPFVKLHGKTLWELAEEVPEVNELINNGMACDTRMVM 180
Query: 962 PAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHG 783
PAF+QGC+ ++SGI LVD+GGG G A++YVVKAFP IK TV D+PHV+ TA + G
Sbjct: 181 PAFVQGCEKVLSGIRKLVDIGGGTGAAISYVVKAFPQIKGTVLDLPHVVATAPE---IPG 237
Query: 782 IEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDEN 603
+E +GGD+F IP ADA+LLKFMLHNW+DEEC +LKRCKEAIP D+GKVII+++VLD++
Sbjct: 238 VEMVGGDMFESIPHADALLLKFMLHNWKDEECIKLLKRCKEAIPTDQGKVIIIEMVLDQD 297
Query: 602 ADNDDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
D++DL QA+LS+DLDMM SSGG+ERTK++W+ LLE +GF+ E IPI AIQS+IVAYP
Sbjct: 298 EDDEDLTQARLSLDLDMMLSSGGKERTKDEWRVLLESAGFSRIEFIPIYAIQSVIVAYP 356
>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
Length = 356
Score = 416 bits (1069), Expect = e-113
Identities = 213/360 (59%), Positives = 269/360 (74%), Gaps = 3/360 (0%)
Frame = -3
Query: 1499 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1320
MEI +E +E EMKYQSQIWN +CG+V+TSVL+C+I LGIFDAIH+SGK M+TLTELS+ +
Sbjct: 1 MEIHLESQEQEMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSGKPMITLTELSSIV 60
Query: 1319 SF--SNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIG 1146
S S+ I+ NLYRL+RYLS M+LI+I SLT SKLLL NQEKSL DW +
Sbjct: 61 SSPSSSSIEPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLA 120
Query: 1145 IGDVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLA 966
I V WHE+SSS S + ++ VHGK EL E PE+++ + AM+SDT++
Sbjct: 121 ISCEMMVVVWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVT 180
Query: 965 MPAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCH 786
PA IQGC I++G++SL+DVGGG G M Y+V+AFPHIK V D+PHV+ A +
Sbjct: 181 KPALIQGCGKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERP--- 237
Query: 785 GIEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDE 606
G+EFI GD+F I ADA+LLK++LHNWED EC N+LKRCKEA+PADKGKVIIMD+V+D+
Sbjct: 238 GVEFISGDIFKSISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDD 297
Query: 605 NADNDDLVQAKLSMDLDMMS-SGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ DN L QAKLS+DL +M+ GGRERTKEDW+NL+EMSGF+ HEIIPI A+ SIIVAYP
Sbjct: 298 D-DNSILTQAKLSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356
>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
Length = 356
Score = 416 bits (1068), Expect = e-113
Identities = 213/360 (59%), Positives = 269/360 (74%), Gaps = 3/360 (0%)
Frame = -3
Query: 1499 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1320
MEI +E +E EMKYQSQIWN +CG+V+TSVL+C+I LGIFDAIH+SGK M+TLTELS+ +
Sbjct: 1 MEIHLESQEQEMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSGKPMITLTELSSIV 60
Query: 1319 SF--SNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIG 1146
S S+ I+ NLYRL+RYLS M+LI+I SLT SKLLL NQEKSL DW +
Sbjct: 61 SSPSSSSIEPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLA 120
Query: 1145 IGDVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLA 966
I V WHE+SSS S + ++ VHGK EL E PE+++ + AM+SDT++
Sbjct: 121 IXCEMMVVVWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVT 180
Query: 965 MPAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCH 786
PA IQGC I++G++SL+DVGGG G M Y+V+AFPHIK V D+PHV+ A +
Sbjct: 181 KPALIQGCGKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERP--- 237
Query: 785 GIEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDE 606
G+EFI GD+F I ADA+LLK++LHNWED EC N+LKRCKEA+PADKGKVIIMD+V+D+
Sbjct: 238 GVEFISGDIFKSISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVIDD 297
Query: 605 NADNDDLVQAKLSMDLDMMS-SGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ DN L QAKLS+DL +M+ GGRERTKEDW+NL+EMSGF+ HEIIPI A+ SIIVAYP
Sbjct: 298 D-DNSILTQAKLSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 286 bits (731), Expect = 5e-74
Identities = 153/354 (43%), Positives = 229/354 (64%), Gaps = 1/354 (0%)
Frame = -3
Query: 1487 MEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSN 1308
ME ++ + Q+++WN + G + VLKC++ L + + IH+SG SM TL+ELS+ L S
Sbjct: 1 MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSM-TLSELSSRLP-SQ 58
Query: 1307 MIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNF 1128
+ LYR++RYL HM L +SI G + L +K L+ +K + + I D +F
Sbjct: 59 PVNEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDF 118
Query: 1127 VATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQ 948
+A WH + S G TA+E G + E PE ++ F++AM++D+ L M A ++
Sbjct: 119 MAPWHYLKDGLSGESG--TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVK 176
Query: 947 GCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIG 768
C I +GI++LVDVGGG GTA+ + AFPHIKCTV+D+PHVI AD + +
Sbjct: 177 ECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVI---ADSPGYSEVHCVA 233
Query: 767 GDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDD 588
GD+F FIP ADAI++K +LH+W+D+EC ILKRCKEA+P GKVII+DIVL+ +++
Sbjct: 234 GDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEH-P 292
Query: 587 LVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ +L++DLDMM ++GG+ERT+E+WK L+ +G+ H+I I A+QS+I AYP
Sbjct: 293 YTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 281 bits (718), Expect = 1e-72
Identities = 156/358 (43%), Positives = 224/358 (62%), Gaps = 1/358 (0%)
Frame = -3
Query: 1499 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1320
MEIQ E + K IW V G + VL+C+I LGI D IH G+ LTL+EL +
Sbjct: 1 MEIQKEGQAAAAK----IWKFVYGFADCLVLRCAIDLGIADIIHKQGEP-LTLSELGAQI 55
Query: 1319 SFSNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIG 1140
+ T +L+RL+RYL HM + ++ G + L +K ++ +KS+ + +
Sbjct: 56 PVQP-VNTDHLHRLMRYLVHMKIFTKETLDGEARYGLAPPAKFIVKGWDKSIVSIILVVT 114
Query: 1139 DVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMP 960
D +F+A WH + S L G+ TA+E G+ + + PE ++ F++ M+ DT L +
Sbjct: 115 DKDFMAPWHCLKDS---LCGEGTAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLIS 171
Query: 959 AFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGI 780
A +Q C + GI SLVDVGGG GTAM + KAFPH+KCT++D+PHVI AD +
Sbjct: 172 ALVQDCKDLFQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVI---ADSPDYPEV 228
Query: 779 EFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENA 600
+ I GD+F IP ADAILLK +LH+W+D EC ILKRCKE++P + GKVII+DIV+D +
Sbjct: 229 DRIAGDMFKHIPSADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDLES 288
Query: 599 DNDDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ L + +LS+DLDMM ++GG+ERT+ +WK LL +GF +I I A+QS+IVAYP
Sbjct: 289 KH-PLTKTRLSLDLDMMVTTGGKERTEAEWKKLLNAAGFPVFKITHISAVQSVIVAYP 345
>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
Length = 359
Score = 278 bits (710), Expect = 1e-71
Identities = 145/353 (41%), Positives = 229/353 (64%), Gaps = 3/353 (0%)
Frame = -3
Query: 1478 EELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIK 1299
+E+ +K Q+Q+WN + G ++ VL+C++ +GI D I ++ + +TL +L+ L +N +
Sbjct: 13 QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA-ITLAQLAAKLPITN-VS 70
Query: 1298 THNLYRLLRYLSHMNLIAISSIQGNDH--FSLTDLSKLLLGNQEKSLKDWAIGIGDVNFV 1125
+ LYR++RYL H+N+I + G +SL + LLL + E+S+ +G+ +F+
Sbjct: 71 SDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFM 130
Query: 1124 ATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQG 945
+WH M G TA+E G + ++ +P+ S+ F++ M+ +T L I+
Sbjct: 131 VSWHFMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIED 188
Query: 944 CDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGG 765
C G+ SLVD+GGG GT + + +AFPHIKCT++D+PHV+ + D IE + G
Sbjct: 189 CRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHD---LPNIEKVPG 245
Query: 764 DLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDL 585
D+F +P A AILLK +LH+W DEEC NILK+CKEAIP + GKVII+D+ L+E + N +L
Sbjct: 246 DMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEES-NHEL 304
Query: 584 VQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ +L +D+DM+ ++GGRERT +DW+NLL+ +GF SH+I PI AIQS+I A+P
Sbjct: 305 TKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 2; Short=4'-OMT2; Short=Ps4'OMT2;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413894|gb|AAP45314.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-
O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 278 bits (710), Expect = 1e-71
Identities = 145/353 (41%), Positives = 229/353 (64%), Gaps = 3/353 (0%)
Frame = -3
Query: 1478 EELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIK 1299
+E+ +K Q+Q+WN + G ++ VL+C++ +GI D I ++ + +TL +L+ L +N +
Sbjct: 13 QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA-ITLAQLAAKLPITN-VS 70
Query: 1298 THNLYRLLRYLSHMNLIAISSIQGNDH--FSLTDLSKLLLGNQEKSLKDWAIGIGDVNFV 1125
+ LYR++RYL H+N+I + G +SL + LLL + E+S+ +G+ +F+
Sbjct: 71 SDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFM 130
Query: 1124 ATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQG 945
+WH M G TA+E G + ++ +P+ S+ F++ M+ +T L I+
Sbjct: 131 VSWHFMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIED 188
Query: 944 CDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGG 765
C G+ SLVD+GGG GT + + +AFPHIKCT++D+PHV+ + D IE + G
Sbjct: 189 CRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHD---LPNIEKVPG 245
Query: 764 DLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDL 585
D+F +P A AILLK +LH+W DEEC NILK+CKEAIP + GKVII+D+ L+E + N +L
Sbjct: 246 DMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEES-NHEL 304
Query: 584 VQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ +L +D+DM+ ++GGRERT +DW+NLL+ +GF SH+I PI AIQS+I A+P
Sbjct: 305 TKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 276 bits (706), Expect = 4e-71
Identities = 152/356 (42%), Positives = 225/356 (63%), Gaps = 3/356 (0%)
Frame = -3
Query: 1487 MEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSN 1308
ME + + Q+ IW + G + VLKC+I L I D IH+ G+ M +L+EL++ L
Sbjct: 1 MEATKSDQANQANIWKLIYGFAESLVLKCAIQLEIADTIHNHGEPM-SLSELASKLPVQP 59
Query: 1307 MIKTHNLYRLLRYLSHMNLI--AISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDV 1134
+ + LYR++RYL HM L +SI G +SL +K L+ EKS+ + I D
Sbjct: 60 -VNSDRLYRVMRYLVHMKLFNKEKTSINGEFKYSLAPPAKFLIKGWEKSMVASILAINDK 118
Query: 1133 NFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAF 954
+F+A WH + S GD A+E GK + E+PE ++ F++AM+ DT L A
Sbjct: 119 DFLAPWHHLKDGLS---GDCDAFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVTSAL 175
Query: 953 IQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEF 774
+ C + GI++LVDVGGG GTA+ + KAFPHIKC+++D+PHVI AD + +
Sbjct: 176 VNDCQSVFKGINTLVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVI---ADSPEIPNVVK 232
Query: 773 IGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADN 594
I GD+F IP ADAIL+K +LH+W D+EC ILK+CKEA+P + GKVII+D+VL+ + +
Sbjct: 233 IEGDMFKAIPSADAILMKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDLTH 292
Query: 593 DDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ +L++DLDMM ++GG+ERT E+WK L++ +GF S +I I A+QS+I A+P
Sbjct: 293 -PYSKIRLTLDLDMMLNTGGKERTVEEWKKLIDAAGFASFKITEISAVQSVIEAFP 347
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 276 bits (705), Expect = 5e-71
Identities = 147/344 (42%), Positives = 220/344 (63%), Gaps = 1/344 (0%)
Frame = -3
Query: 1457 QSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKTHNLYRL 1278
Q+++WN + G ++ VLK ++ L + + IH+ G M TL+ELS L S + LYR+
Sbjct: 12 QAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPM-TLSELSLHLP-SQPVNQDALYRV 69
Query: 1277 LRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATWHEMSSS 1098
LRYL HM L SSI G + L +K L+ +K + + I D +F+A WH +
Sbjct: 70 LRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWHYLKEG 129
Query: 1097 CSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDIIMSGIS 918
TA+E G ++ E PE ++ F++ M++DT L M A ++ C + GI+
Sbjct: 130 ILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSMFDGIT 189
Query: 917 SLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDLFSFIPPA 738
++VDVGGG GTA+ + KAFPHIKCTV+D+PHVI AD I I GD+F +IP A
Sbjct: 190 TIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVI---ADSPGYTEINSIQGDMFKYIPNA 246
Query: 737 DAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQAKLSMDL 558
DAI++K +LH+W+D+EC ILKRCK+A+P D GKVII+DI+LD +++ + +L++DL
Sbjct: 247 DAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEH-PYTKMRLTLDL 305
Query: 557 DMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
DMM ++GG+ERT+E+WK L+ +G+ ++I I A+QS+I AYP
Sbjct: 306 DMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 275 bits (702), Expect = 1e-70
Identities = 153/360 (42%), Positives = 222/360 (61%), Gaps = 3/360 (0%)
Frame = -3
Query: 1499 MEIQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSL 1320
MEI E + E+ +IW +T VL+C+I GI D IH G+ LTL EL +
Sbjct: 1 MEIPKEVQADEV----EIWKFGYDFADTLVLRCAIEFGIADIIHKQGEP-LTLFELGAQI 55
Query: 1319 SFSNMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIG 1140
+ T +L+RL+RY+ HM + ++ G + + L+ K L+ +KS+ + I
Sbjct: 56 PVQP-VNTDHLHRLMRYMVHMKIFTKETLGGEEXYGLSPHGKFLVKGWDKSMASAILAIT 114
Query: 1139 DVNFVATWHEMSSSCSK--LPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLA 966
D +F A WH C K L G+ TA+E GK + + PE ++ F++ M+ DT+
Sbjct: 115 DEDFFAPWH-----CLKDVLAGEGTAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFI 169
Query: 965 MPAFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCH 786
IQ C + GI S+VDVGGG GTAM + KAFPH+KCT++D+PHVI AD
Sbjct: 170 TSVLIQDCKDVFQGIKSVVDVGGGTGTAMRDIAKAFPHLKCTIYDLPHVI---ADSPDYP 226
Query: 785 GIEFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDE 606
++ I GD+F IP ADAILLK++LH+W+D EC ILKRCKE++P + GKVII+DIVLD
Sbjct: 227 EVDRIAGDMFKHIPSADAILLKWILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLDP 286
Query: 605 NADNDDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ D L +A+L +DLDMM +GG+ER++ +WK LL +GF ++I+ + A+QS+I+AYP
Sbjct: 287 ES-KDPLTKARLRLDLDMMVYTGGKERSEAEWKKLLNAAGFPGYKILHVAAVQSVIMAYP 345
>gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Papaver
pseudo-orientale]
Length = 343
Score = 274 bits (701), Expect = 1e-70
Identities = 152/351 (43%), Positives = 223/351 (63%), Gaps = 2/351 (0%)
Frame = -3
Query: 1475 ELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKT 1296
+++ + Q++IW + G + VLKC++ L I + +H++ K M +L+EL++ L + +
Sbjct: 4 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPM-SLSELASKLPVAQPVNE 62
Query: 1295 HNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATW 1116
L+R++RYL HM L I + +SL +K LL EKS+ D + I D +F+A W
Sbjct: 63 DRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 120
Query: 1115 HEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDI 936
H + L G+ A+E GK + E+PE ++ F+ AM+ DT L A C
Sbjct: 121 HHLGDG---LTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 177
Query: 935 IMS-GISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDL 759
I S GI +LVDVGGG GTA+ + KAFP IKCT++D+PHVI AD + I I GD+
Sbjct: 178 IFSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVI---ADSPEIPNITKISGDM 234
Query: 758 FSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQ 579
F IP ADAI +K +LH+W DEEC ILKRCKEA+P D GKVII+D+V+D ++ + +
Sbjct: 235 FKSIPSADAIFMKCILHDWSDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTH-PYAK 292
Query: 578 AKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L++DLDMM ++GG+ERTKE+WK L + +GF SH++ I A+QS+I AYP
Sbjct: 293 IRLTLDLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 343
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 274 bits (700), Expect = 2e-70
Identities = 152/351 (43%), Positives = 222/351 (63%), Gaps = 2/351 (0%)
Frame = -3
Query: 1475 ELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKT 1296
+++ + Q++IW + G + VLKC++ L I + +H++ K M +L+EL++ L +
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPM-SLSELASKLPVPQPVNE 64
Query: 1295 HNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATW 1116
L+R++RYL HM L I + +SL +K LL EKS+ D + I D +F+A W
Sbjct: 65 DRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 1115 HEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDI 936
H + L G+ A+E GK + E+PE ++ F+ AM+ DT L A C
Sbjct: 123 HHLGDG---LTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 935 IMS-GISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDL 759
I S GI +LVDVGGG GTA+ + KAFP IKCT++D+PHVI AD + I I GD+
Sbjct: 180 IFSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVI---ADSPEIPNITKISGDM 236
Query: 758 FSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQ 579
F IP ADAI +K +LH+W DEEC ILKRCKEA+P D GKVII+D+V+D ++ + +
Sbjct: 237 FKSIPSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDMDSTH-PYAK 294
Query: 578 AKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L++DLDMM ++GG+ERTKE+WK L + +GF SH++ I A+QS+I AYP
Sbjct: 295 IRLTLDLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345
>sp|Q7XB11.1|4OMT1_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 1; Short=4'-OMT1; Short=Ps4'OMT1;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413892|gb|AAP45313.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 274 bits (700), Expect = 2e-70
Identities = 149/358 (41%), Positives = 229/358 (63%), Gaps = 3/358 (0%)
Frame = -3
Query: 1493 IQMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSF 1314
I E E+++K Q+Q+WN + G ++ VL+C++ +GI D I ++ S +TL+EL + L
Sbjct: 5 IDAETHEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGS-ITLSELVSKLPL 63
Query: 1313 SNMIKTHNLYRLLRYLSHMNLIAISSIQ-GNDH-FSLTDLSKLLLGNQEKSLKDWAIGIG 1140
SN + + NLYRLLRYL H+N++ + G D +SL + LLL + E+S+ +G+
Sbjct: 64 SN-VNSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILGLS 122
Query: 1139 DVNFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMP 960
+F+ W+ + G TA+E G + ++ +P S+ FD+ + +T L
Sbjct: 123 QKDFLFVWNFVKEGLGT--GSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLTK 180
Query: 959 AFIQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGI 780
+ C G+ SLVDVGGG GT + + +AFPHIKCT++D+PHVI + D I
Sbjct: 181 TLLVDCRDTFQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPN---I 237
Query: 779 EFIGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENA 600
+ GD+F +P A +LLK +LH+W DE C NILK+CKEAIP + GKVII+D+ L+E +
Sbjct: 238 LKVPGDMFMSVPSAQVLLLKCVLHDWTDEHCVNILKKCKEAIPKETGKVIIVDVALEEES 297
Query: 599 DNDDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
++ +L +A+L +D+DM+ ++GGRERT EDW+NLL+ +GF SH+I PI AIQS+I A+P
Sbjct: 298 EH-ELTKARLILDIDMLVNTGGRERTAEDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 354
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
somniferum]
Length = 346
Score = 273 bits (698), Expect = 3e-70
Identities = 151/351 (43%), Positives = 223/351 (63%), Gaps = 2/351 (0%)
Frame = -3
Query: 1475 ELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKT 1296
+++ + Q++IW + G + VLKC++ L I + +H++ K M +L+EL++ L + +
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPM-SLSELASKLPVAQPVNE 64
Query: 1295 HNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATW 1116
L+R++RYL HM L I + +SL +K LL EKS+ D + I D +F+A W
Sbjct: 65 DRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 1115 HEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDI 936
H + L G+ A+E GK + E+PE ++ F+ AM+ DT L A C
Sbjct: 123 HHLGDG---LTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 935 IMS-GISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDL 759
I S GIS+LVDVGGG GTA+ + KAFP IKCT++D+PHVI AD + I I GD+
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVI---ADSPEIPNITKISGDM 236
Query: 758 FSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQ 579
F IP ADAI +K +LH+W D+EC ILKRCKEA+P GKVII+D+V+D ++ + +
Sbjct: 237 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALP-KVGKVIIVDVVIDMDSTH-PYAK 294
Query: 578 AKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L++DLDMM ++GG+ERTKE+WK L + +GF SH++ I A+QS+I AYP
Sbjct: 295 IRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 272 bits (696), Expect = 5e-70
Identities = 148/356 (41%), Positives = 223/356 (62%), Gaps = 3/356 (0%)
Frame = -3
Query: 1487 MEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSN 1308
ME ++ + Q+++WN + G + VLKC++ L + IH+ GK M TL+EL++ L
Sbjct: 1 MEAQKENISSQAKLWNFIYGFAESLVLKCAVELDFANIIHNHGKPM-TLSELASQLPVMQ 59
Query: 1307 MIKTHNLYRLLRYLSHMNLIA--ISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDV 1134
+ T++LYR++RYL H+N+ + G + L +K L+ + + +GI D
Sbjct: 60 PVNTNSLYRVMRYLVHINIFTKTLDENDGETKYGLAAPAKFLVKGWDNCMVGSILGITDK 119
Query: 1133 NFVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAF 954
F+ W+ + + PG TA+E GK+ +E E+PE ++ F+ AM+ DT++ M A
Sbjct: 120 VFMEPWYYIKDELA--PGTGTAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIMSAL 177
Query: 953 IQGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEF 774
I C +GI +LVDVGGG GTA + +AFP+IKCTV+D+PHVI AD I
Sbjct: 178 ISECKDKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVI---ADSPVYPEINR 234
Query: 773 IGGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADN 594
+ GD+F IP ADAIL+K +LH+WED+EC ILKRCKEA+P D GKVII+D+VLD +++
Sbjct: 235 VSGDMFKCIPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLDGESEH 294
Query: 593 DDLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+ +L+ DLDMM ++ G+ERTKE WK L + +G+ + I I A+QS+I A+P
Sbjct: 295 -PYTKVRLNSDLDMMLNTEGKERTKEGWKKLFKAAGYRDYNITQISALQSVIEAFP 349
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 271 bits (692), Expect = 2e-69
Identities = 150/351 (42%), Positives = 222/351 (63%), Gaps = 2/351 (0%)
Frame = -3
Query: 1475 ELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKT 1296
+++ + Q++IW + G + VLKC++ L I + +H++ K M +L+EL++ L + +
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPM-SLSELASKLPVAQPVNE 64
Query: 1295 HNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATW 1116
L+R++RYL HM L I + +SL +K LL EKS+ D + I D +F+A W
Sbjct: 65 DRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 1115 HEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDI 936
H + L G+ A+E GK + +PE ++ F+ AM+ DT L A C
Sbjct: 123 HHLGDG---LTGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 935 IMS-GISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDL 759
I S GIS+LVDVGGG GTA+ + KAFP IKCT++D+PHVI AD + I I GD+
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVI---ADSPEIPNITKISGDM 236
Query: 758 FSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQ 579
F IP ADAI +K +LH+W D+EC ILKRCKEA+P GKVII+D+V+D ++ + +
Sbjct: 237 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTH-PYAK 294
Query: 578 AKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L++DLDMM ++GG+ERTKE+WK L + +GF SH++ I A+QS+I AYP
Sbjct: 295 IRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=Ps6OMT
gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
6-O-methyltransferase [Papaver somniferum]
gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
6-O-methyltransferase [synthetic construct]
Length = 346
Score = 271 bits (692), Expect = 2e-69
Identities = 150/351 (42%), Positives = 222/351 (63%), Gaps = 2/351 (0%)
Frame = -3
Query: 1475 ELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKT 1296
+++ + Q++IW + G + VLKC++ L I + +H++ K M +L+EL++ L + +
Sbjct: 6 KIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPM-SLSELASKLPVAQPVNE 64
Query: 1295 HNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATW 1116
L+R++RYL HM L I + +SL +K LL EKS+ D + I D +F+A W
Sbjct: 65 DRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 122
Query: 1115 HEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDI 936
H + L G+ A+E GK + +PE ++ F+ AM+ DT L A C
Sbjct: 123 HHLGDG---LTGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKS 179
Query: 935 IMS-GISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDL 759
I S GIS+LVDVGGG GTA+ + KAFP IKCT++D+PHVI AD + I I GD+
Sbjct: 180 IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVI---ADSPEIPNITKISGDM 236
Query: 758 FSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQ 579
F IP ADAI +K +LH+W D+EC ILKRCKEA+P GKVII+D+V+D ++ + +
Sbjct: 237 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTH-PYAK 294
Query: 578 AKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L++DLDMM ++GG+ERTKE+WK L + +GF SH++ I A+QS+I AYP
Sbjct: 295 IRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
flavum subsp. glaucum]
Length = 348
Score = 266 bits (679), Expect = 5e-68
Identities = 142/355 (40%), Positives = 227/355 (63%), Gaps = 1/355 (0%)
Frame = -3
Query: 1490 QMEDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFS 1311
Q +D +++K Q+ +W + G ++ VL+C++ LGIFD I D+ + L +L++ L S
Sbjct: 5 QGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDII-DNNNQPIPLADLASKLPIS 63
Query: 1310 NMIKTHNLYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVN 1131
N + NL+R+LRYL M L++ ++ +D ++L ++KL+L N+++S+ +G+ +
Sbjct: 64 N-VNLDNLFRILRYLVKMELLSYAA---DDKYALEPIAKLVLRNEKRSMVPMVLGMTQKD 119
Query: 1130 FVATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFI 951
F+ WH M + +TA+E G +E P+ S+ F++ M+ +T L + I
Sbjct: 120 FMTPWHSMKDGLTD--NGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLI 177
Query: 950 QGCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFI 771
G + I SLVDVGGG GT + + AFPHIKCT+FD+PHVI + D IE I
Sbjct: 178 SGSKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPN---IERI 234
Query: 770 GGDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADND 591
GGD+F +P A AILLK +LH+W DE+ ILK+C++A+P D GKVII+D+ LDE++D+
Sbjct: 235 GGDMFKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDH- 293
Query: 590 DLVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
+L +L +D+DM+ ++GG+ERTKEDW+ L++ +GF+ +I I AIQS+I +P
Sbjct: 294 ELSSTRLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348
>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 353
Score = 265 bits (676), Expect = 1e-67
Identities = 143/354 (40%), Positives = 226/354 (63%), Gaps = 2/354 (0%)
Frame = -3
Query: 1484 EDEELEMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNM 1305
E +E+++K Q+ +WN + G ++ VL+C++ +GI D I + S +++TEL++ L +N
Sbjct: 9 ESQEVDIKAQAHLWNIIYGFADSLVLRCAVEIGIADIIKSNNGS-ISVTELASKLPITN- 66
Query: 1304 IKTHNLYRLLRYLSHMNLIA-ISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNF 1128
+ + NLYR+LRYL HM ++ +S +SL ++ LLL + E+S+ +G+ +F
Sbjct: 67 VNSDNLYRVLRYLVHMGILKEVSDSNEVKLYSLQPVATLLLRDAERSMVPIILGMTQKDF 126
Query: 1127 VATWHEMSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQ 948
+ WH M L D TA+E G ++ PE S F++ M+ +T L + I
Sbjct: 127 MIPWHFMKEG---LGNDTTAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRLLTKSLID 183
Query: 947 GCDIIMSGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIG 768
GC G++SL DVGGG GT + + AFP IKC+V+D+PHVI ++ + IE I
Sbjct: 184 GCRDTFEGLTSLCDVGGGNGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPN---IERIP 240
Query: 767 GDLFSFIPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDD 588
GD+F +P A AILLK +LH+W DEEC NIL +C+EA+P D GKVII+D+ L+E + + +
Sbjct: 241 GDMFKSVPSAQAILLKLILHDWTDEECVNILIKCREAVPKDTGKVIIVDVALEEESQH-E 299
Query: 587 LVQAKLSMDLDMM-SSGGRERTKEDWKNLLEMSGFTSHEIIPILAIQSIIVAYP 429
L + +L +D+DM+ ++GGRER+++DW+ LL+ +GF H+I I AIQS+I A+P
Sbjct: 300 LTKTRLILDIDMLVNTGGRERSEDDWEKLLKRAGFRGHKIRHIAAIQSVIEAFP 353
>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum flavum]
Length = 346
Score = 263 bits (671), Expect = 4e-67
Identities = 140/349 (40%), Positives = 222/349 (63%), Gaps = 2/349 (0%)
Frame = -3
Query: 1469 EMKYQSQIWNHVCGSVNTSVLKCSISLGIFDAIHDSGKSMLTLTELSNSLSFSNMIKTHN 1290
++ Q+++WN++ G ++ VLKC++ L + + IHD G M TL+ELS+ L S +
Sbjct: 3 DLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPM-TLSELSSRLP-SQPVNQDA 60
Query: 1289 LYRLLRYLSHMNLIAISSIQGNDHFSLTDLSKLLLGNQEKSLKDWAIGIGDVNFVATWHE 1110
LYR++RYL H+ L SSI G + L +K L+ + + + + D +F+A +H
Sbjct: 61 LYRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHY 120
Query: 1109 MSSSCSKLPGDLTAWETVHGKEGYELNVEDPEFSERFDKAMSSDTTLAMPAFIQGCDIIM 930
+ +TA+E G +E E PE ++ F++ M++++ L M ++ C
Sbjct: 121 IKDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDCSSTF 180
Query: 929 SGISSLVDVGGGIGTAMTYVVKAFPHIKCTVFDVPHVIHTAADPAKCHGIEFIGGDLFSF 750
G+++LVDVGGG GT + Y+ KAFP+IK TV+D+PHVI AD I F+ GD+F +
Sbjct: 181 EGLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVI---ADAPGDTEINFVEGDMFKY 237
Query: 749 IPPADAILLKFMLHNWEDEECKNILKRCKEAIPADKGKVIIMDIVLDENADNDDLVQAKL 570
IP ADAIL+K +LH+W+D EC ILKRCK+A+P D GKVII+D+VL+ +++ + +L
Sbjct: 238 IPNADAILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEH-PYTKMRL 296
Query: 569 SMDLDMM-SSGGRERTKEDWKNLL-EMSGFTSHEIIPILAIQSIIVAYP 429
++DLDMM ++ G+ERT+EDWK L+ + +G+ ++I I A+QS+I AYP
Sbjct: 297 TLDLDMMLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYP 345