BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Papaver30_contig00055141
         (1122 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002318771.1| hypothetical protein POPTR_0012s10860g [Popu...    77   3e-11
ref|XP_007029557.1| Uncharacterized protein TCM_025447 [Theobrom...    75   1e-10
ref|XP_012829574.1| PREDICTED: uncharacterized protein LOC105950...    72   6e-10
gb|EYU17458.1| hypothetical protein MIMGU_mgv1a025045mg, partial...    72   6e-10
ref|XP_007020843.1| Uncharacterized protein TCM_030892 [Theobrom...    72   8e-10
ref|XP_007020841.1| Uncharacterized protein TCM_030890 [Theobrom...    71   2e-09
ref|XP_007029555.1| Uncharacterized protein TCM_025442 [Theobrom...    68   1e-08
ref|XP_012853108.1| PREDICTED: uncharacterized protein LOC105972...    67   2e-08
ref|XP_010917353.1| PREDICTED: F-box/kelch-repeat protein At1g57...    64   2e-07
dbj|BAD25853.1| hypothetical protein [Oryza sativa Japonica Grou...    64   3e-07
gb|EAZ44584.1| hypothetical protein OsJ_29204 [Oryza sativa Japo...    61   1e-06

>ref|XP_002318771.1| hypothetical protein POPTR_0012s10860g [Populus trichocarpa]
            gi|222859444|gb|EEE96991.1| hypothetical protein
            POPTR_0012s10860g [Populus trichocarpa]
          Length = 329

 Score = 77.0 bits (188), Expect = 3e-11
 Identities = 73/299 (24%), Positives = 124/299 (41%)
 Frame = -1

Query: 1092 DDCFLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVF 913
            DDCFL NP+S++ IQLP+L            F   D V            P   +C  +F
Sbjct: 78   DDCFLLNPISTKKIQLPSL---------APDFTWTDCVLSSP--------PHRPECVVMF 120

Query: 912  FIFRRMRDNGEVSRDTHILVFCRPGDKQWRIKELDCRFNPDGDFSEFIVSLLCFQGKLYA 733
              F     N         +  C+PGD +W  ++L+   +   D S+  VS+    G +Y 
Sbjct: 121  LNFGYGILN---------VKSCKPGDVEWTGQDLELH-DEWFDDSDCGVSVGVHNGDIYI 170

Query: 732  FCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWLESGN 553
              C E+ +  +   K     +VD         KV+    PL    +  SY     +E+  
Sbjct: 171  LTCYEH-LYSVKFNKSCGITLVD--------LKVDDRTSPLTR--KFHSYCPTYLVETCG 219

Query: 552  EILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCY 373
            E L+++          ++   ++KLDF+   W+ +K+L D  +F+ ++            
Sbjct: 220  EFLRVHCYILHGQLMDIS---VYKLDFNERVWIRIKNLKDQAIFIGSS------------ 264

Query: 372  STCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIM 196
                  CS  +  +Q   ++ TLPED+TLY+++++     V  PC  +   W    WI+
Sbjct: 265  GAQVLACSTKESRIQGNRIYLTLPEDRTLYVYDLDLCGLEVCLPCPNVKADWIQNDWIL 323


>ref|XP_007029557.1| Uncharacterized protein TCM_025447 [Theobroma cacao]
            gi|508718162|gb|EOY10059.1| Uncharacterized protein
            TCM_025447 [Theobroma cacao]
          Length = 314

 Score = 75.1 bits (183), Expect = 1e-10
 Identities = 71/299 (23%), Positives = 116/299 (38%)
 Frame = -1

Query: 1089 DCFLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVFF 910
            +CFL N  S ETIQLP L+              F L            N      C +  
Sbjct: 78   NCFLLNLSSMETIQLPPLN--------------FKLAIGILTAPPSDPN------CRILL 117

Query: 909  IFRRMRDNGEVSRDTHILVFCRPGDKQWRIKELDCRFNPDGDFSEFIVSLLCFQGKLYAF 730
            I              H  +FC PGD ++  ++++          +F+ S+    GK+Y  
Sbjct: 118  I-----------DGNHDFIFCSPGDSEFSKQKVE----------DFLYSMTTLGGKIYCL 156

Query: 729  CCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWLESGNE 550
               E  +  ++          +  +   TK      +  +    +  SY +E +     E
Sbjct: 157  TLPEYSLLTMEF---------EGSSLRFTKLNTIRNESNIFHIEDNRSYLIEFF----GE 203

Query: 549  ILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCYS 370
            +L +    S + F       +FK DF    WV +KS+GD+ +FL     T+D      Y 
Sbjct: 204  MLLVCKYLSLKSFEWTHDIGVFKFDFCGREWVEVKSIGDNAIFL-----TDDF-----YG 253

Query: 369  TCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIMM 193
            TC     +    + R  ++YT  ED+ LY++++ED + T   PC  +  P  L  W M+
Sbjct: 254  TCYPVVDS----ITRNSIYYTYSEDKNLYVYDLEDQSITTHLPCPIVSRPCSLHYWCML 308


>ref|XP_012829574.1| PREDICTED: uncharacterized protein LOC105950755 [Erythranthe
            guttatus]
          Length = 337

 Score = 72.4 bits (176), Expect = 6e-10
 Identities = 70/304 (23%), Positives = 126/304 (41%), Gaps = 8/304 (2%)
 Frame = -1

Query: 1089 DCFLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVFF 910
            DC L+NP+S + I LP LD         + +  F +             P   DC  +F 
Sbjct: 74   DCCLFNPISKDKIDLPKLDNG-------DSYNKFIMTK----------RPTEPDCYIIF- 115

Query: 909  IFRRMRDNGEVSRDTHILVFCRPGDKQWRIKELDCR---FNPDGDFSEFIVSLLCFQGKL 739
                       S + H L FCR GD+++    LD     F+ +G F   +++++CF+GK+
Sbjct: 116  -----------SSERHELSFCRIGDEEYVKVPLDGHDIGFDEEGGFRADLMAIVCFRGKI 164

Query: 738  YAFCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPD--FPLISGGEEDSYYMEDWL 565
            Y F         I+  K      VDK  ++     +   D  +P I     +S     WL
Sbjct: 165  YGF---------IEPNKFVTINFVDKSLEYERPVTLMNEDGTWPWIIPRVSNSRVNGIWL 215

Query: 564  ---ESGNEILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTED 394
                S +E+L +         +  +   +F +D + MT + ++++G+  +F+     T D
Sbjct: 216  IESFSTDELLLVLKTYPCLTVKDSSEFRVFSVDTNGMTCMEVENIGNRTIFV-----TRD 270

Query: 393  LTSRRCYSTCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWF 214
                        YC    +G++   ++YT   D+   +F++ED + T      ++P+P  
Sbjct: 271  ---------DGGYCCPTPIGIKPNSIYYTNIRDRNYNVFDLEDRSKT------SMPIPGG 315

Query: 213  LPTW 202
            +  W
Sbjct: 316  VAVW 319


>gb|EYU17458.1| hypothetical protein MIMGU_mgv1a025045mg, partial [Erythranthe
            guttata]
          Length = 297

 Score = 72.4 bits (176), Expect = 6e-10
 Identities = 70/304 (23%), Positives = 126/304 (41%), Gaps = 8/304 (2%)
 Frame = -1

Query: 1089 DCFLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVFF 910
            DC L+NP+S + I LP LD         + +  F +             P   DC  +F 
Sbjct: 34   DCCLFNPISKDKIDLPKLDNG-------DSYNKFIMTK----------RPTEPDCYIIF- 75

Query: 909  IFRRMRDNGEVSRDTHILVFCRPGDKQWRIKELDCR---FNPDGDFSEFIVSLLCFQGKL 739
                       S + H L FCR GD+++    LD     F+ +G F   +++++CF+GK+
Sbjct: 76   -----------SSERHELSFCRIGDEEYVKVPLDGHDIGFDEEGGFRADLMAIVCFRGKI 124

Query: 738  YAFCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPD--FPLISGGEEDSYYMEDWL 565
            Y F         I+  K      VDK  ++     +   D  +P I     +S     WL
Sbjct: 125  YGF---------IEPNKFVTINFVDKSLEYERPVTLMNEDGTWPWIIPRVSNSRVNGIWL 175

Query: 564  ---ESGNEILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTED 394
                S +E+L +         +  +   +F +D + MT + ++++G+  +F+     T D
Sbjct: 176  IESFSTDELLLVLKTYPCLTVKDSSEFRVFSVDTNGMTCMEVENIGNRTIFV-----TRD 230

Query: 393  LTSRRCYSTCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWF 214
                        YC    +G++   ++YT   D+   +F++ED + T      ++P+P  
Sbjct: 231  ---------DGGYCCPTPIGIKPNSIYYTNIRDRNYNVFDLEDRSKT------SMPIPGG 275

Query: 213  LPTW 202
            +  W
Sbjct: 276  VAVW 279


>ref|XP_007020843.1| Uncharacterized protein TCM_030892 [Theobroma cacao]
            gi|508720471|gb|EOY12368.1| Uncharacterized protein
            TCM_030892 [Theobroma cacao]
          Length = 741

 Score = 72.0 bits (175), Expect = 8e-10
 Identities = 88/362 (24%), Positives = 136/362 (37%), Gaps = 19/362 (5%)
 Frame = -1

Query: 1083 FLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVFFIF 904
            FLWNPVSSE I+LP LD       KP+Q                   PD      + F  
Sbjct: 85   FLWNPVSSELIKLPPLD------LKPDQ---------RITTGSLLSPPDNPGSMVLVF-- 127

Query: 903  RRMRDNGEVSRDTHILVFCRPGDKQWR---IKELDCRFN-----PDGDFSEFIVSLLCFQ 748
                            +FC+ GDK W     +E+D         P         S + ++
Sbjct: 128  ---------ENIVKSFIFCKLGDKMWTQIPAEEMDTEMQIIDDEPSASNRLLYSSPVNYK 178

Query: 747  GKLYAFCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPDFPLIS---GGEEDSYY- 580
            GK Y                    V + +Q + + + K E   F  ++        SY  
Sbjct: 179  GKCY--------------------VPMSRQIKVIDQVKPEYFMFRSLNCMLPNRLSSYSD 218

Query: 579  -MEDWL-ESGNEILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNM 406
             +E +L ES  E+  I +        +V    I +L+FS+M W  ++S      FLC   
Sbjct: 219  CLESYLVESYGELCLIEVTWGGVNVSQVLDIEISRLNFSTMEWSQVRSAKGRAFFLCRT- 277

Query: 405  DTEDLTSRRCYSTCSAYCSAADMGLQRGCLF-YTLPEDQTLYIFEVEDNATTVFFPCLTL 229
                       +  +  C   D GL+ G ++ +T+  D+ LY F +ED + +V  P   L
Sbjct: 278  -----------AVYAISCPTNDSGLEGGFVYIFTVGSDRCLYSFNIEDKSISVSLPWENL 326

Query: 228  PVPWFLPTWIMMPIT---VNRQAAGRRKRMTDLLLSQDTTETSIEEENMVWVTN-SIGES 61
            P  W  P W+M  ++    NR+  G +    ++   ++  E   EE     + N S   S
Sbjct: 327  PKSWDTPFWVMPDLSSLFSNRKPEGFQILSKEVKPEEEEEEIETEERKGKEILNFSPDNS 386

Query: 60   EA 55
            EA
Sbjct: 387  EA 388


>ref|XP_007020841.1| Uncharacterized protein TCM_030890 [Theobroma cacao]
           gi|508720469|gb|EOY12366.1| Uncharacterized protein
           TCM_030890 [Theobroma cacao]
          Length = 542

 Score = 70.9 bits (172), Expect = 2e-09
 Identities = 60/229 (26%), Positives = 97/229 (42%), Gaps = 9/229 (3%)
 Frame = -1

Query: 855 VFCRPGDKQWR---IKELDCRFNPDGDFSEFIVSLLC-----FQGKLYAFCCDENWVNEI 700
           +FC  GDK+W     +E+D +     +       LLC     ++GK Y     +  +  I
Sbjct: 149 IFCELGDKKWTEIPAEEMDVKMQIMDNEPSARNRLLCSSPVNYKGKWYVPMSRK--IKVI 206

Query: 699 DIQKLWHHVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWL-ESGNEILKIYLNCS 523
           D  K  H V+                   +IS     SY  + +L ES  E+  I +   
Sbjct: 207 DQVKPKHMVLRSLNC--------------MISNRSWYSYCEDRYLVESCGELCVIEVTWG 252

Query: 522 PRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCYSTCSAYCSAA 343
                +V +  I +LDFS+M W  ++S  D   F+              +S  +  C A 
Sbjct: 253 GVNACQVLNIEISRLDFSTMEWSQVRSAKDRAFFISN------------FSVYAISCPAN 300

Query: 342 DMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIM 196
           + G++ G ++YT+  D+ LY F +ED + +V  P + LP  W  P W+M
Sbjct: 301 ESGIEGGFVYYTVGTDRCLYSFNIEDKSISVSLPWVNLPKSWSTPFWVM 349


>ref|XP_007029555.1| Uncharacterized protein TCM_025442 [Theobroma cacao]
            gi|508718160|gb|EOY10057.1| Uncharacterized protein
            TCM_025442 [Theobroma cacao]
          Length = 359

 Score = 68.2 bits (165), Expect = 1e-08
 Identities = 69/285 (24%), Positives = 121/285 (42%), Gaps = 2/285 (0%)
 Frame = -1

Query: 1089 DCFLWNPVSSETIQLP--NLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTV 916
            DCFL N  S ETIQLP  NLD      T P                    +P+    C +
Sbjct: 5    DCFLLNLASMETIQLPPLNLDMAVGILTTPPS------------------DPN----CRI 42

Query: 915  FFIFRRMRDNGEVSRDTHILVFCRPGDKQWRIKELDCRFNPDGDFSEFIVSLLCFQGKLY 736
             FI     D  +       L+ C PGD ++  ++++          + ++++  F GK Y
Sbjct: 43   LFI-----DGNDD------LIICSPGDSEYSKQKME----------DPVLTMTRFGGKTY 81

Query: 735  AFCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWLESG 556
              C      + + I+       ++  +   TK  + V +   +   E+ + Y+ D+    
Sbjct: 82   --CLTPPVYSLLTIE-------LEGSSPRFTKL-ITVGNESKLFSFEQTAPYLLDFF--- 128

Query: 555  NEILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRC 376
             E+  +    S +      +  +FK DF +  WV +KS+G++ +FL          +  C
Sbjct: 129  GEMFLVCKCSSLKSSDWATNFGVFKFDFDAREWVEVKSIGNNAIFL----------TDYC 178

Query: 375  YSTCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFP 241
            Y TC      AD  ++R  ++YT P+D+ LY++++E  + T F P
Sbjct: 179  YGTCYP---VADHSMRRNSIYYTQPDDRNLYVYDLEYQSITTFLP 220


>ref|XP_012853108.1| PREDICTED: uncharacterized protein LOC105972679 [Erythranthe
            guttatus]
          Length = 336

 Score = 67.4 bits (163), Expect = 2e-08
 Identities = 63/305 (20%), Positives = 128/305 (41%), Gaps = 1/305 (0%)
 Frame = -1

Query: 1089 DCFLWNPVSSETIQLPNLDRQSFPTTKPEQFYMFDLVXXXXXXXXXTCNPDGYDCCTVFF 910
            DC L+NPVS + I LP          K +  Y ++ +            P   DC   + 
Sbjct: 74   DCCLFNPVSKDKIDLPK---------KLDNGYFYNKLIMTKP-------PTEPDC---YI 114

Query: 909  IFRRMRDNGEVSRDTHILVFCRPGDKQW-RIKELDCRFNPDGDFSEFIVSLLCFQGKLYA 733
            IF   RD          L FCR GD+++  ++E +  F+ +  F  ++++++CF+GK+Y 
Sbjct: 115  IFSSERDG---------LSFCRIGDEEYVTVEEHEIGFDEEDGFMGYLMAIVCFRGKIYG 165

Query: 732  FCCDENWVNEIDIQKLWHHVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWLESGN 553
            F   + +V    + K   +   ++    + +   +    P    G  +S ++     S +
Sbjct: 166  FIEPDKFVTINFVDKSLEY---ERPVTLMNEDGTQPWFIPRAFRGRINSIWLIKSC-STD 221

Query: 552  EILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCY 373
            E+L +         +  +   +F +D ++MT + ++++G+  +F+  +            
Sbjct: 222  ELLLVLKTYPCLTVKDSSEFRVFSVDTNAMTCMEVENIGNRSIFVTLD------------ 269

Query: 372  STCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIMM 193
                 YC    +G++   ++YT   D+   +F++ED + T   P L     W    W+  
Sbjct: 270  --DGGYCCPTPIGIKPNSIYYTNIRDRNYNVFDLEDRSKT-SMPILGGVAVWSKAVWVQP 326

Query: 192  PITVN 178
             +  N
Sbjct: 327  QMLKN 331


>ref|XP_010917353.1| PREDICTED: F-box/kelch-repeat protein At1g57790-like [Elaeis
           guineensis]
          Length = 311

 Score = 64.3 bits (155), Expect = 2e-07
 Identities = 54/223 (24%), Positives = 90/223 (40%), Gaps = 2/223 (0%)
 Frame = -1

Query: 858 LVFCRPGDKQWRIKELDCRFNPDGDFSEFIVSLLCFQGKLYAFCCDENWVNEIDIQKLWH 679
           L FC  GDK+W    L   F+ D     F+ S++  QGKLYA   +EN +          
Sbjct: 121 LFFCHVGDKEWT--RLTVNFSHDF----FVYSIVNCQGKLYALTFEENIM---------- 164

Query: 678 HVVVDKQTQFLTKFKVEVPDFPLISGGEEDSYYMEDWLESGNEILKIYLNCSPRGFRKVA 499
             ++D  +  +    +EV  F  +        Y  + +E+  +I  +       G   V 
Sbjct: 165 --LIDTTSSSMNVEIMEVESFTSLHSYPS---YFPNLVEACGDIFLVLRYSRSWGGGHVV 219

Query: 498 STHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCYSTCSAYCSAADMGLQRGC 319
           +  + KLDFS + W  ++S+GD   FL                      SA + G+Q   
Sbjct: 220 TVDVHKLDFSKLRWERVESIGDRAFFLS--------------GAFGISLSATESGVQPNH 265

Query: 318 LFYTLP--EDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIM 196
           +++  P  + + LYIF +++   +   PC  +   W    W M
Sbjct: 266 IYFIQPCNDAERLYIFSLDERVISFDMPCPNIRRTWDYLWWTM 308


>dbj|BAD25853.1| hypothetical protein [Oryza sativa Japonica Group]
           gi|49388758|dbj|BAD25956.1| hypothetical protein [Oryza
           sativa Japonica Group]
          Length = 340

 Score = 63.5 bits (153), Expect = 3e-07
 Identities = 40/135 (29%), Positives = 69/135 (51%)
 Frame = -1

Query: 588 SYYMEDWLESGNEILKIYLNCSPRGFRKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTN 409
           S Y   +LES  ++  +         +KV+   + KLD S   WV + ++G+   F+   
Sbjct: 218 SIYTSYFLESCGDLFMLSYKHPVLCAQKVSQIEVHKLDLSRRIWVKVSTIGNMAFFV--- 274

Query: 408 MDTEDLTSRRCYSTCSAYCSAADMGLQRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTL 229
            D+ D       S  SA  +A D+GL+R C++Y  P+D+ LYI+++E   T+V  P + L
Sbjct: 275 -DSTD-------SGVSASLNAEDVGLKRNCIYYVRPKDKGLYIYDIERGTTSVHNPGVDL 326

Query: 228 PVPWFLPTWIMMPIT 184
              +  P  +M P++
Sbjct: 327 -ADYLTPDIMMTPLS 340


>gb|EAZ44584.1| hypothetical protein OsJ_29204 [Oryza sativa Japonica Group]
          Length = 269

 Score = 61.2 bits (147), Expect = 1e-06
 Identities = 35/109 (32%), Positives = 60/109 (55%)
 Frame = -1

Query: 510 RKVASTHIFKLDFSSMTWVLLKSLGDHVLFLCTNMDTEDLTSRRCYSTCSAYCSAADMGL 331
           +KV+   + KLD S   WV + ++G+   F+    D+ D       S  SA  +A D+GL
Sbjct: 173 QKVSQIEVHKLDLSRRIWVKVSTIGNMAFFV----DSTD-------SGVSASLNAEDVGL 221

Query: 330 QRGCLFYTLPEDQTLYIFEVEDNATTVFFPCLTLPVPWFLPTWIMMPIT 184
           +R C++Y  P+D+ LYI+++E   T+V  P + L   +  P  +M P++
Sbjct: 222 KRNCIYYVRPKDKGLYIYDIERGTTSVHNPGVDL-ADYLTPDIMMTPLS 269