BLASTX nr result
ID: Papaver30_contig00040965
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Papaver30_contig00040965
(803 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;... 298 2e-78
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum] 295 3e-77
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;... 293 7e-77
gb|ACO90230.1| cheilanthifoline synthase-like protein [Papaver b... 292 2e-76
sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt... 270 7e-70
gb|ACO90235.1| stylopine synthase-like protein [Papaver bracteatum] 245 3e-62
gb|ADB89214.1| stylopine synthase [Papaver somniferum] 244 4e-62
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt... 243 9e-62
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh... 241 3e-61
gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis] 240 7e-61
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta] 239 1e-60
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g... 238 3e-60
gb|ACO90219.1| stylopine synthase [Eschscholzia californica] 236 1e-59
gb|AGC29954.1| CYP719A24 [Podophyllum peltatum] 236 2e-59
gb|AHF65153.1| stylopine synthase, partial [synthetic construct] 235 2e-59
sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; ... 235 2e-59
gb|AJW76496.1| methylenedioxy bridge-forming enzyme [Dysosma ver... 234 5e-59
gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum] 233 9e-59
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum] 232 2e-58
sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; ... 229 1e-57
>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A14 [Argemone mexicana]
gi|148841094|gb|ABR14722.1| cheilanthifoline synthase
[Argemone mexicana]
Length = 494
Score = 298 bits (764), Expect = 2e-78
Identities = 152/249 (61%), Positives = 193/249 (77%), Gaps = 2/249 (0%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSYPTGT--EWPAGPKTLPLIGNLHQLWGDLFHV 586
MDE ++I STVI+VL IAKFL + S + + EWP GPK LP+IGNLHQL GD+FHV
Sbjct: 1 MDETIWLII-STVIIVLGIAKFLLGKSSSSSLSTMEWPVGPKKLPIIGNLHQLGGDVFHV 59
Query: 585 VLANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGND 406
VLANLAKV+G VFTIW+GSW P+IIVSD++ A EVL+ KSSDYS+R+ P+ ++S
Sbjct: 60 VLANLAKVYGSVFTIWVGSWRPMIIVSDIDKAWEVLVNKSSDYSARDMPDITKIISANWK 119
Query: 405 SINGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPL 226
+I+ SD GPFWHNLRKGL AL PLNV SQ HLQERDM+NLI SM ++ASR+ GILKPL
Sbjct: 120 NISCSDSGPFWHNLRKGLQGVALTPLNVASQYHLQERDMKNLINSMYKDASRKNGILKPL 179
Query: 225 DYVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHR 46
DY+K+E +RLL+RLIFGQDF DE +VG+HH + L+ I G+A+LADAFK E +P H+
Sbjct: 180 DYLKEETVRLLSRLIFGQDF-QDEKLVVGMHHALDDLVRISGYASLADAFKFCENLPSHK 238
Query: 45 RYIRGLNDV 19
+ IR +++V
Sbjct: 239 KSIREVHEV 247
>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
Length = 492
Score = 295 bits (754), Expect = 3e-77
Identities = 142/240 (59%), Positives = 185/240 (77%)
Frame = -3
Query: 738 VIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANLAKVH 559
+I V++ A+AK L +KS + EWP+GPKTLP+IGNLHQL G+ FHV LANLAKV+
Sbjct: 7 LITCGVVVAFALAKLLFGKKSSMSTMEWPSGPKTLPIIGNLHQLGGEAFHVCLANLAKVY 66
Query: 558 GGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGSDCGP 379
GGVFTIW+GSW P I++SDV+ A EVL+ KSSDYS+R+ P+ ++S +I+ D GP
Sbjct: 67 GGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSARDMPDITKIISANWKNISHGDSGP 126
Query: 378 FWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKKEAIR 199
FWHNLRKGL AL P NV SQ HLQERDMQNLI+SM++ AS++ GILKPLDYVK+E +R
Sbjct: 127 FWHNLRKGLQGVALTPFNVASQYHLQERDMQNLIKSMKKKASQKNGILKPLDYVKEETVR 186
Query: 198 LLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRGLNDV 19
LL+RLIFGQDF DEDF+VG+H + L+SI G+A+LADAFK E +P H++ +R ++ +
Sbjct: 187 LLSRLIFGQDFV-DEDFVVGMHQALDELVSISGYASLADAFKFCENLPSHKKTVRAVHAI 245
>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A5 [Eschscholzia californica]
gi|209571368|dbj|BAG75113.1| cheilanthifoline synthase
[Eschscholzia californica]
Length = 490
Score = 293 bits (751), Expect = 7e-77
Identities = 146/248 (58%), Positives = 187/248 (75%)
Frame = -3
Query: 753 ENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLAN 574
E SL V+ +TV++V AIAK L S T EWP GPK LP+IGNLHQL G+ FHVVLAN
Sbjct: 2 EESLWVVTATVVVVFAIAKLLKKSSSIST-MEWPKGPKKLPIIGNLHQLGGEAFHVVLAN 60
Query: 573 LAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSING 394
LAK+HG V TIW+G+W P+I++SD++ A EVL+ KSSDY+ R++PE ++S +I+
Sbjct: 61 LAKIHGTVMTIWVGAWRPMIVISDIDKAWEVLVNKSSDYAGRDFPEITKIISANWKNISC 120
Query: 393 SDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVK 214
SD GPFW NLRKGL GAL PLNV SQ LQERDM+NLI SMQE AS+ GILKPLDY+K
Sbjct: 121 SDSGPFWQNLRKGLQGGALAPLNVISQYQLQERDMKNLITSMQEKASKNNGILKPLDYLK 180
Query: 213 KEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIR 34
+E IRLL+RLIFGQ F NDE+F+ G+H + L+ I G+A+LADAFK E +P H++ IR
Sbjct: 181 EETIRLLSRLIFGQSF-NDENFVKGVHLALDDLVRISGYASLADAFKFCENLPSHKKSIR 239
Query: 33 GLNDVGEQ 10
+++V E+
Sbjct: 240 EVHEVNER 247
>gb|ACO90230.1| cheilanthifoline synthase-like protein [Papaver bracteatum]
Length = 271
Score = 292 bits (748), Expect = 2e-76
Identities = 141/240 (58%), Positives = 184/240 (76%)
Frame = -3
Query: 738 VIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANLAKVH 559
+I V++ A+AK L +KS + EWPAGPKTLP+IGNLHQL G+ FHV LANLAKV+
Sbjct: 7 LITCGVVVAFALAKLLFGKKSSLSTMEWPAGPKTLPIIGNLHQLGGEAFHVCLANLAKVY 66
Query: 558 GGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGSDCGP 379
GGVFTIW+GSW P I++SDV+ A EVL+ KSSDYS+R+ P+ ++S +I+ D P
Sbjct: 67 GGVFTIWVGSWRPFIVISDVDKAWEVLVNKSSDYSARDMPDITKIISANWKNISHGDSSP 126
Query: 378 FWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKKEAIR 199
FWHNLRKGL AL P NV +Q HLQERDMQNLI+SM+E AS++ GILKPLDYVK+E +R
Sbjct: 127 FWHNLRKGLQGVALTPFNVAAQHHLQERDMQNLIKSMKEKASQKNGILKPLDYVKEETVR 186
Query: 198 LLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRGLNDV 19
LL+RLIFGQ+F DEDF+VG+H + L+SI G+A+LADAFK E +P H++ +R ++ +
Sbjct: 187 LLSRLIFGQEFV-DEDFVVGMHQALDELVSISGYASLADAFKFCENLPSHKKTVRAVHAI 245
>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
[Papaver somniferum] gi|827520611|gb|AKJ85663.1|
salutaridine synthase [synthetic construct]
Length = 505
Score = 270 bits (691), Expect = 7e-70
Identities = 134/248 (54%), Positives = 184/248 (74%), Gaps = 4/248 (1%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSY---PTGTEWPAGPKTLPLIGNLHQLWGDL-F 592
++ N +IA TVI+V A+ KF+ + S+ TEWPAGPKTLP+IGNLHQL G +
Sbjct: 6 IEGNDFWMIACTVIIVFALVKFMFSKISFYQSANTTEWPAGPKTLPIIGNLHQLGGGVPL 65
Query: 591 HVVLANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEG 412
V LANLAKV+GG FTIW+GSW P+I++SD+++AREVL+ KS+DYS+R+ P+ L +++
Sbjct: 66 QVALANLAKVYGGAFTIWIGSWVPMIVISDIDNAREVLVNKSADYSARDVPDILKIITAN 125
Query: 411 NDSINGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILK 232
+I D GPFWHNL+KGL S +NP NV S S LQE+DMQNLI+SMQE AS+ GI+K
Sbjct: 126 GKNIADCDSGPFWHNLKKGLQS-CINPSNVMSLSRLQEKDMQNLIKSMQERASQHNGIIK 184
Query: 231 PLDYVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPR 52
PLD+ K+ ++RLL+R+IFG DFSN ED ++G+ + ++ I G A+LADAFKI +Y+P
Sbjct: 185 PLDHAKEASMRLLSRVIFGHDFSN-EDLVIGVKDALDEMVRISGLASLADAFKIAKYLPS 243
Query: 51 HRRYIRGL 28
R+ IR +
Sbjct: 244 QRKNIRDM 251
>gb|ACO90235.1| stylopine synthase-like protein [Papaver bracteatum]
Length = 274
Score = 245 bits (625), Expect = 3e-62
Identities = 112/250 (44%), Positives = 173/250 (69%)
Frame = -3
Query: 750 NSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANL 571
NS I+ ST++++ I + ++ + WP GPKTLP+IGN+H L G VVL NL
Sbjct: 8 NSWILTTSTLVVITIITMLMVFKRKKSSTMVWPTGPKTLPIIGNMHVLGGTALQVVLHNL 67
Query: 570 AKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGS 391
AK+HG V TIW+GSW PVI+VSD++ A EVL+ KSSDYS+R+ PE ++S +I+ S
Sbjct: 68 AKIHGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDMPEITKIISADWKTISTS 127
Query: 390 DCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKK 211
D GP W NLRKGL + AL+P N+ +QS QE+D+ +I+S+++ A+ GI+KP+D++KK
Sbjct: 128 DSGPGWSNLRKGLQNVALSPHNLAAQSQFQEKDITQMIQSLKKEAASNNGIVKPIDHLKK 187
Query: 210 EAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRG 31
+R+++RLIFGQDF +D+ ++ +HH + LI + G+A LA+AF +Y+P H++ +R
Sbjct: 188 ATLRVISRLIFGQDF-DDDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRD 246
Query: 30 LNDVGEQAAK 1
+ + ++ K
Sbjct: 247 VEEANKRVKK 256
>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
Length = 494
Score = 244 bits (624), Expect = 4e-62
Identities = 112/250 (44%), Positives = 172/250 (68%)
Frame = -3
Query: 750 NSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANL 571
N I+ ST++++ I + ++ + WP GPKTLP+IGN+H L G VVL NL
Sbjct: 8 NPWILTTSTLVVITIITMLMVFKRKKSSTMVWPTGPKTLPIIGNMHVLGGTALQVVLHNL 67
Query: 570 AKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGS 391
AK+HG V TIW+GSW PVI+VSD++ A EVL+ KSSDYS+R+ PE ++S +I+ S
Sbjct: 68 AKIHGNVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDMPEITKIISADWKTISTS 127
Query: 390 DCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKK 211
D GP W NLRKGL + AL+P N+ +QS QE+D+ +I+S+++ A+ GI+KP+D++KK
Sbjct: 128 DSGPGWSNLRKGLQNVALSPHNLAAQSQFQEKDITKMIQSLKKEAASNNGIVKPIDHLKK 187
Query: 210 EAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRG 31
+RL++RLIFGQDF +D+ ++ +HH + LI + G+A LA+AF +Y+P H++ +R
Sbjct: 188 ATLRLISRLIFGQDF-DDDKYVDDMHHAIEELIRVSGYAQLAEAFYYAKYLPSHKKAVRD 246
Query: 30 LNDVGEQAAK 1
+ + ++ K
Sbjct: 247 VEEANQRVKK 256
>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
Full=(S)-tetrahydrocolumbamine oxidase
(methylenedioxy-bridge-forming); AltName:
Full=Cytochrome P450 719A1 [Coptis japonica]
gi|15822820|dbj|BAB68769.1| methylenedioxy
bridge-forming enzyme [Coptis japonica]
Length = 491
Score = 243 bits (621), Expect = 9e-62
Identities = 119/241 (49%), Positives = 164/241 (68%), Gaps = 2/241 (0%)
Frame = -3
Query: 759 MDENSLIVIASTVIM--VLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHV 586
M+ N L+V A+ I+ I + L S P +WP+GP+ LP+IGNLHQL D+ HV
Sbjct: 1 MEMNPLLVCATVAIVFATTTIIRILFSSSSLPQ-MKWPSGPRKLPIIGNLHQLGDDVLHV 59
Query: 585 VLANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGND 406
LA LAKVHG V TIW+GSW PVI++SD+ A EVL+ KS+DY +R+ PE + S
Sbjct: 60 ALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWH 119
Query: 405 SINGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPL 226
+I+ SD G FW N+RKGL SGA+ PLNV +Q+ QERDM+ LI++M + A+ GI+KPL
Sbjct: 120 TISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPL 179
Query: 225 DYVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHR 46
D++KK +RLL RLIFGQ F +D FI +H+ + +I I G+A LA+AF +Y+P H+
Sbjct: 180 DHIKKNTVRLLTRLIFGQAF-DDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHK 238
Query: 45 R 43
+
Sbjct: 239 K 239
>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
Full=Cytochrome P450 719A13 [Argemone mexicana]
gi|148841092|gb|ABR14721.1| stylopine synthase [Argemone
mexicana]
Length = 504
Score = 241 bits (616), Expect = 3e-61
Identities = 115/234 (49%), Positives = 168/234 (71%), Gaps = 2/234 (0%)
Frame = -3
Query: 729 STVIMVLAIAKFLALRKSYPTGTE--WPAGPKTLPLIGNLHQLWGDLFHVVLANLAKVHG 556
+T+++ L F+ LR++ + ++ WP GPKTLP+IGN++ L G HVVL NLAK +G
Sbjct: 22 TTILLSLLFTIFIILRRNKSSSSKMVWPTGPKTLPIIGNMNILGGTALHVVLHNLAKTYG 81
Query: 555 GVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGSDCGPF 376
V TIW+GSW PVI+VSD++ A EVL+ KSSDYS+R+ PE + + +I+ SD GPF
Sbjct: 82 NVMTIWIGSWRPVIVVSDIDRAWEVLVNKSSDYSARDMPEITKLATADWKTISSSDSGPF 141
Query: 375 WHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKKEAIRL 196
W NLRKGL + AL+P N++SQS QERD+ I++++E A GI+KPLD++KK +RL
Sbjct: 142 WTNLRKGLQNVALSPQNLSSQSKFQERDIIKTIQNLKEEAKMNNGIVKPLDHLKKAMVRL 201
Query: 195 LNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIR 34
++RLI+GQDF NDE ++ +HHT+ LI + G+A LA+AF +Y+P H++ +R
Sbjct: 202 ISRLIYGQDFDNDE-YVEEMHHTIEELIRVSGYARLAEAFYYAKYLPSHKKAVR 254
>gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]
Length = 491
Score = 240 bits (613), Expect = 7e-61
Identities = 117/240 (48%), Positives = 165/240 (68%), Gaps = 3/240 (1%)
Frame = -3
Query: 753 ENSLIVIASTVIMVLA---IAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVV 583
E S +++ +TV +V A I + L S P +WP+GP+ LP+IGNLHQL D+ HV
Sbjct: 2 EMSPLLVCATVAIVFATTTIIRILFSSSSLPQ-MKWPSGPRKLPIIGNLHQLGDDVLHVA 60
Query: 582 LANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDS 403
LA LAKVHG + TIW+GSW PVI++SD+ A EVL+ KS+DY +R+ PE + S +
Sbjct: 61 LAKLAKVHGSMMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGARDMPEITKIASASWHT 120
Query: 402 INGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLD 223
I+ SD GPFW N+RKGL SGA+ P NV +Q+ QER M+ LI++M + A+ GI+KPLD
Sbjct: 121 ISTSDAGPFWQNVRKGLQSGAMGPFNVAAQNQHQERGMKRLIKAMSDEAANNNGIVKPLD 180
Query: 222 YVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRR 43
++KK +RLL RLIFGQ+F +D FI +H+ + +I I G+A LA+AF +Y+P H++
Sbjct: 181 HIKKNTVRLLTRLIFGQNF-DDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKK 239
>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 491
Score = 239 bits (611), Expect = 1e-60
Identities = 114/243 (46%), Positives = 165/243 (67%), Gaps = 1/243 (0%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSYPTGTE-WPAGPKTLPLIGNLHQLWGDLFHVV 583
M+ + L +I STV +VL I + + S + + WP+GPK LP+IGNLH L GDL HV
Sbjct: 1 MEGSFLWLIGSTVAIVLVITTIVRMSSSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVT 60
Query: 582 LANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDS 403
LA LAKVHG V TIW+G W P+I++SD+NSA EVL++KS++Y R+ P+ L+ G ++
Sbjct: 61 LAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNN 120
Query: 402 INGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLD 223
I+ SD GPFWHN+RKGL +GAL+PLNV +Q+ +E D++ LI M A + GI+KPLD
Sbjct: 121 ISMSDIGPFWHNVRKGLQNGALSPLNVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD 180
Query: 222 YVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRR 43
++KK RLL RLIFG F ND+ F+ + + + ++ + G+A L +AF +Y+P ++
Sbjct: 181 HMKKNTARLLGRLIFGPTFDNDK-FVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKK 239
Query: 42 YIR 34
R
Sbjct: 240 VAR 242
>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
Length = 492
Score = 238 bits (608), Expect = 3e-60
Identities = 111/241 (46%), Positives = 169/241 (70%), Gaps = 2/241 (0%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSYPTGTE--WPAGPKTLPLIGNLHQLWGDLFHV 586
M+ SL ++++T+ +V+AI + + S + ++ WP GP LP+IGNLHQL GD+ HV
Sbjct: 1 MELVSLWLVSATLALVVAITILIPMVTSSSSFSQMKWPTGPNKLPIIGNLHQLGGDVLHV 60
Query: 585 VLANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGND 406
LA LA+VHG V T+W+G+W P+I++SD++ A EVL+ KSSDY +R+ PE + S
Sbjct: 61 ALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVLVNKSSDYGARDMPEITKIASASWH 120
Query: 405 SINGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPL 226
+I+ SD G FW +RKGL SGA+ PLN+ +QS QERDM+ LI+++++ A++ I+KPL
Sbjct: 121 TISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQERDMKRLIQALRDEAAQNNNIVKPL 180
Query: 225 DYVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHR 46
D++KK +RLL RLIFGQ F +D+ F+ +H + +I I G+A LA+AF +Y+P H+
Sbjct: 181 DHIKKNTVRLLTRLIFGQTF-DDDKFVESMHFEIDDIIRISGYARLAEAFYYAKYLPSHK 239
Query: 45 R 43
+
Sbjct: 240 K 240
>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
Length = 495
Score = 236 bits (603), Expect = 1e-59
Identities = 115/246 (46%), Positives = 167/246 (67%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVL 580
M N I+ A+ ++++I F + S WPAGPKTLP+IGN+H L G VVL
Sbjct: 8 MMNNPWILTATATTLLISIFLFFTRKSSKMV---WPAGPKTLPIIGNMHLLGGTALQVVL 64
Query: 579 ANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSI 400
NLAKVHG V TIW+GSW PVI+VSD+ A EVL+ KSSDYS+R+ P+ ++S +I
Sbjct: 65 HNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSARDMPDITKIISADWKTI 124
Query: 399 NGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDY 220
+ SD GP W NLRKGL + AL+P N+ +Q QE+DM +I+++QE A GI+KPLD+
Sbjct: 125 STSDSGPHWTNLRKGLQNVALSPHNLAAQFQFQEKDMTKMIQTLQEEARNNNGIVKPLDH 184
Query: 219 VKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRY 40
+KK +RL++RL+FGQDF+ND+ ++ +H + LI + G+A LA+AF +Y+P H++
Sbjct: 185 MKKATLRLISRLVFGQDFNNDK-YVDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKA 243
Query: 39 IRGLND 22
+R + +
Sbjct: 244 VREVEE 249
>gb|AGC29954.1| CYP719A24 [Podophyllum peltatum]
Length = 489
Score = 236 bits (601), Expect = 2e-59
Identities = 115/240 (47%), Positives = 165/240 (68%)
Frame = -3
Query: 753 ENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLAN 574
E S++ ++ST+I+ LAI L+ + +WP GPKTLP+IGNLHQL GD H+VLA
Sbjct: 4 ETSVLGLSSTLIIALAITVIFLLKAKSSSAIKWPPGPKTLPIIGNLHQLGGDELHIVLAK 63
Query: 573 LAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSING 394
LA+VHG + TIW+ PVI+VSDVNS EVL++KSSDY++R+ E ++S + SIN
Sbjct: 64 LARVHGAIMTIWMAK-KPVIVVSDVNSVWEVLVSKSSDYAARDAAEISKIVSASSHSINT 122
Query: 393 SDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVK 214
SD GP+W LR+GLT G L PLN+++Q +Q+RDMQ +IR MQ++A+ G++KPLD++K
Sbjct: 123 SDSGPYWQTLRRGLTHGPLGPLNISAQIPIQQRDMQRVIREMQQDAAANGGVIKPLDHLK 182
Query: 213 KEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIR 34
+ + RL++RLIFG F ND + +H V L GG A L AF +Y+P ++R ++
Sbjct: 183 RSSTRLVSRLIFGDTFDND-PYNDSMHEVVQDLNRFGGIALLEQAFSFAKYLPSYKRGVK 241
>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
Length = 511
Score = 235 bits (600), Expect = 2e-59
Identities = 107/219 (48%), Positives = 158/219 (72%)
Frame = -3
Query: 657 WPAGPKTLPLIGNLHQLWGDLFHVVLANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVL 478
WP GPKTLP+IGN+H L G VVL NLAK+HG V TIW+GSW PVI+VSD++ A EVL
Sbjct: 53 WPTGPKTLPIIGNMHVLGGTALQVVLHNLAKIHGNVMTIWIGSWRPVIVVSDIDRAWEVL 112
Query: 477 LTKSSDYSSRNYPEYLTVLSEGNDSINGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQE 298
+ KSSDYS+R+ PE ++S +I+ SD GP W NLRKGL + AL+P N+ +QS QE
Sbjct: 113 VNKSSDYSARDMPEITKIISADWKTISTSDSGPGWSNLRKGLQNVALSPHNLAAQSQFQE 172
Query: 297 RDMQNLIRSMQENASREKGILKPLDYVKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHH 118
+D+ +I+S+++ A+ GI+KP+D++KK +RL++RLIFGQDF +D+ ++ +HH +
Sbjct: 173 KDITKMIQSLKKEAASNNGIVKPIDHLKKATLRLISRLIFGQDF-DDDKYVDDMHHAIEE 231
Query: 117 LISIGGFANLADAFKIGEYVPRHRRYIRGLNDVGEQAAK 1
LI + G+A LA+AF +Y+P H++ +R + + ++ K
Sbjct: 232 LIRVSGYAQLAEAFYYAKYLPSHKKAVRDVEEANQRVKK 270
>sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; Short=STS; AltName:
Full=Cytochrome P450 719A2; Short=EcCYP719A2;
Short=EcCYPB gi|63108256|dbj|BAD98250.1| stylopine
synthase [Eschscholzia californica]
Length = 495
Score = 235 bits (600), Expect = 2e-59
Identities = 114/246 (46%), Positives = 167/246 (67%)
Frame = -3
Query: 759 MDENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVL 580
M N I+ A+ ++++I F + S WPAGPKTLP+IGN+H L G VVL
Sbjct: 8 MMNNPWILTATATTLLISIFLFFTRKSSKMV---WPAGPKTLPIIGNMHLLGGTALQVVL 64
Query: 579 ANLAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSI 400
NLAKVHG V TIW+GSW PVI+VSD+ A EVL+ KSSDYS+R+ P+ ++S +I
Sbjct: 65 HNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVLVNKSSDYSARDMPDITKIISADWKTI 124
Query: 399 NGSDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDY 220
+ SD GP W NLRKGL + AL+P N+ +Q QE+DM +I++++E A GI+KPLD+
Sbjct: 125 STSDSGPHWTNLRKGLQNVALSPHNLAAQFQFQEKDMTKMIQTLEEEARNNNGIVKPLDH 184
Query: 219 VKKEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRY 40
+KK +RL++RL+FGQDF+ND+ ++ +H + LI + G+A LA+AF +Y+P H++
Sbjct: 185 MKKATLRLISRLVFGQDFNNDK-YVDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKA 243
Query: 39 IRGLND 22
+R + +
Sbjct: 244 VREVEE 249
>gb|AJW76496.1| methylenedioxy bridge-forming enzyme [Dysosma versipellis]
Length = 489
Score = 234 bits (597), Expect = 5e-59
Identities = 114/240 (47%), Positives = 164/240 (68%)
Frame = -3
Query: 753 ENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLAN 574
E S + ++ST+I+ LA+A L+ + +WP GPKTLP+IGNLHQL GD H+VLA
Sbjct: 4 EMSALAMSSTLILALAMAAIFLLKAKSSSAIKWPPGPKTLPIIGNLHQLGGDELHIVLAK 63
Query: 573 LAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSING 394
LA+VHG + TIW+ PVI+VSDVNS EVL++KSSDY++R+ E ++S + SIN
Sbjct: 64 LARVHGAIMTIWMAK-KPVIVVSDVNSVWEVLVSKSSDYAARDAAEISKIVSASSHSINT 122
Query: 393 SDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVK 214
SD GP+W LR+GLT G L PLN+++Q +Q+RDMQ +IR MQ+ + G++KPLD++K
Sbjct: 123 SDSGPYWQTLRRGLTHGPLGPLNISAQIPIQQRDMQRVIREMQQGGAVNGGVIKPLDHLK 182
Query: 213 KEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIR 34
+ + RL++RLIFG +F ND + +H V L GG A L AF +Y+P ++R ++
Sbjct: 183 RSSTRLVSRLIFGDNFDND-PYNDSMHEVVQDLNRFGGIALLEQAFSFAKYLPSYKRGVK 241
>gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum]
Length = 489
Score = 233 bits (595), Expect = 9e-59
Identities = 114/240 (47%), Positives = 165/240 (68%)
Frame = -3
Query: 753 ENSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLAN 574
E S++ ++ST+I+ LA+A + + +WP GPKTLP+IGNLHQL GD H+VLA
Sbjct: 4 EMSVLAMSSTLILALAMALIFLFKAKSSSAIKWPPGPKTLPIIGNLHQLGGDELHIVLAK 63
Query: 573 LAKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSING 394
LA+VHG + TIW+ PVI+VSDVNS EVL++KSSDY++R+ E ++S + SIN
Sbjct: 64 LARVHGAIMTIWMAK-KPVIVVSDVNSVWEVLVSKSSDYAARDAAEISKIVSASSHSINT 122
Query: 393 SDCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVK 214
SD GP+W LR+GLT G L PLN+++Q +Q+RDMQ +IR MQ++A+ GI+KPLD++K
Sbjct: 123 SDSGPYWQTLRRGLTHGPLGPLNISAQIPIQQRDMQRVIREMQQDAAANGGIIKPLDHLK 182
Query: 213 KEAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIR 34
+ + RL++RLIFG F ND + +H V L GG A L AF +++P ++R ++
Sbjct: 183 RSSTRLVSRLIFGDTFDND-PYNDSMHEVVQDLNRFGGIALLEQAFSFAKHLPSYKRGVK 241
>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
Length = 488
Score = 232 bits (592), Expect = 2e-58
Identities = 113/241 (46%), Positives = 164/241 (68%)
Frame = -3
Query: 744 LIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANLAK 565
++ + ST++ V A + R+ + TEWP GPKTLP+IGNLH L G HVVL LA+
Sbjct: 8 ILTLISTILAVFAAVLIIFRRRISASTTEWPVGPKTLPIIGNLHILGGTALHVVLHKLAE 67
Query: 564 VHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGSDC 385
V+G V TIW+GSW PVIIVSD + A EVL+ KSSDYS+R PE + + +I+ SD
Sbjct: 68 VYGSVMTIWIGSWKPVIIVSDFDRAWEVLVNKSSDYSAREMPEITKIGTANWRTISSSDS 127
Query: 384 GPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKKEA 205
GPFW LRKGL S AL+P ++ SQ+ QERD+ LI+++++ A+ G++KPLD++KK
Sbjct: 128 GPFWATLRKGLQSVALSPQHLASQTAHQERDIIKLIKNLKDEAALNSGMVKPLDHLKKAT 187
Query: 204 IRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRGLN 25
+RL++RLI+GQDF +D+ ++ +H + LI I G+A LA+ F +Y+P H+R + G
Sbjct: 188 VRLISRLIYGQDF-DDDKYVEDMHDVIEFLIRISGYAQLAEVFYYAKYLPSHKRAVTGAE 246
Query: 24 D 22
+
Sbjct: 247 E 247
>sp|Q50LH4.1|C7193_ESCCA RecName: Full=(S)-stylopine synthase 2; Short=STS; AltName:
Full=Cytochrome P450 719A3; Short=EcCYP719A3;
Short=EcCYPA gi|63108254|dbj|BAD98249.1| stylopine
synthase [Eschscholzia californica]
Length = 495
Score = 229 bits (585), Expect = 1e-57
Identities = 111/244 (45%), Positives = 167/244 (68%)
Frame = -3
Query: 750 NSLIVIASTVIMVLAIAKFLALRKSYPTGTEWPAGPKTLPLIGNLHQLWGDLFHVVLANL 571
N ++ A++ ++++I FL R+ P WP GPKTLP+IGN+H L G VVL NL
Sbjct: 11 NPWVLFATSATLLISI--FLFFRRKSPN-MAWPEGPKTLPIIGNMHLLGGTALQVVLYNL 67
Query: 570 AKVHGGVFTIWLGSWNPVIIVSDVNSAREVLLTKSSDYSSRNYPEYLTVLSEGNDSINGS 391
AKVHG V TIW+GSW PVI+VSD+ A EVL+ KSSDYS+R+ P+ +++ +I+ S
Sbjct: 68 AKVHGRVMTIWIGSWRPVIVVSDIEQAWEVLVNKSSDYSARDMPDITKIVTADWRTISTS 127
Query: 390 DCGPFWHNLRKGLTSGALNPLNVTSQSHLQERDMQNLIRSMQENASREKGILKPLDYVKK 211
D GP W NLRKGL + A++P N+ +Q QE+D+ +I+ +++ A GI+KPLD++KK
Sbjct: 128 DSGPHWSNLRKGLQNIAISPNNLAAQFQFQEKDIIKMIQILEQEAKDNNGIVKPLDHLKK 187
Query: 210 EAIRLLNRLIFGQDFSNDEDFIVGIHHTVHHLISIGGFANLADAFKIGEYVPRHRRYIRG 31
IRL++RL+FG+DF D+ ++ +HH + LI I G+A LA+AF +Y+P HR+ +R
Sbjct: 188 ATIRLISRLVFGRDFEEDK-YVEDMHHAIEELIRISGYARLAEAFYYAKYLPSHRKAVRY 246
Query: 30 LNDV 19
+ ++
Sbjct: 247 VEEL 250